LOCUS       NZ_CP031853             4685 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 121 plasmid pKP121-4, complete
            sequence.
ACCESSION   NZ_CP031853
VERSION     NZ_CP031853.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09912217
            Assembly: GCF_004801235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ruan,Z., Sun,Q., Jia,H., Huang,C., Zhou,W., Xie,X. and Zhang,J.
  TITLE     Emergence of a ST2570 Klebsiella pneumoniae isolate carrying mcr-1
            and blaCTX-M-14 recovered from a bloodstream infection in China
  JOURNAL   Clin Microbiol Infect 25 (7), 916-918 (2019)
   PUBMED   30771524
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Ruan,Z. and Sun,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2018) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3; SPAdes v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio RSII; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 03:22:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,600
            CDSs (total)                      :: 5,475
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 63 of 159
            Pseudo Genes (incomplete)         :: 91 of 159
            Pseudo Genes (internal stop)      :: 22 of 159
            Pseudo Genes (multiple problems)  :: 16 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031853.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="121"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP121-4"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.2859343 N 120.1944756 E"
                     /collection_date="2018-01-30"
                     /collected_by="Qingyang Sun"
     gene            complement(205..351)
                     /locus_tag="D1Y71_RS30420"
                     /old_locus_tag="D1Y71_28850"
     CDS             complement(205..351)
                     /locus_tag="D1Y71_RS30420"
                     /old_locus_tag="D1Y71_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071786219.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERRAKAPLAAATGKGVYKRLLV
                     QSS"
                     /name="replication initiation protein"
     gene            768..959
                     /locus_tag="D1Y71_RS28580"
                     /old_locus_tag="D1Y71_28860"
     CDS             768..959
                     /locus_tag="D1Y71_RS28580"
                     /old_locus_tag="D1Y71_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            959..1360
                     /locus_tag="D1Y71_RS28585"
                     /old_locus_tag="D1Y71_28865"
     CDS             959..1360
                     /locus_tag="D1Y71_RS28585"
                     /old_locus_tag="D1Y71_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_080843463.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2166..2438
                     /locus_tag="D1Y71_RS28595"
                     /old_locus_tag="D1Y71_28875"
     CDS             2166..2438
                     /locus_tag="D1Y71_RS28595"
                     /old_locus_tag="D1Y71_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(2563..2638)
                     /locus_tag="D1Y71_RS28600"
                     /old_locus_tag="D1Y71_28880"
     tRNA            complement(2563..2638)
                     /locus_tag="D1Y71_RS28600"
                     /old_locus_tag="D1Y71_28880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2603..2605),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(2809..2895)
                     /locus_tag="D1Y71_RS28605"
                     /old_locus_tag="D1Y71_28885"
     tRNA            complement(2809..2895)
                     /locus_tag="D1Y71_RS28605"
                     /old_locus_tag="D1Y71_28885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2859..2861),aa:Ile,seq:aat)
                     /name="tRNA-Ile"
     gene            complement(3016..4527)
                     /locus_tag="D1Y71_RS28610"
                     /old_locus_tag="D1Y71_28890"
     CDS             complement(3016..4527)
                     /locus_tag="D1Y71_RS28610"
                     /old_locus_tag="D1Y71_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023325450.1"
                     /translation="MTRINLDNILNEQGPCLTSELAETLVKNFGFTPAAARKRISRGTN
                     KIRKLSYIIFPHRARFIYLKKDYASEKYWNALYSSLRKENSSYYMAIRCIKSRGGMIKR
                     DEFGILCGSPFRQKNHIPYESIISSLIKSEIILEISSASGDRYLYLKEFEGSEHFLLEG
                     QNKKELISGIMIEQSRTWLKQLGLVSFGKVKAMGDDNNHPRVGTFEWHITGPSYTHPLT
                     KKYDNKTKPGFVVCDLNTQPITTLDDISIFIKKMDMTISMKNIGNCIFVYISNGYTEEA
                     LYLAKSKGVMAITYNNIFGKRNITAIDKISEILGNKWHDENLSGELARLTKELNERNGI
                     TQNLKGRLFEFICSDIKRRIEPTSRLILGREYITTQNERAEADITSIRDEVSISFIECK
                     GIKRESNLDDKQVDQWLDVRIPRLINYCKSHPDYKKLKKSFELWITGGLTEQSEIRIEH
                     FKKTHPKVDLIIKKPEDLIRHVKEINDRSIKKMLFEYFLKMERAQ"
                     /name="hypothetical protein"
     Typing          complement(184..723)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.087"
                     /coverage="90.0"
                     /evalue="3.17e-180"
ORIGIN
        1 aatgagcttg ctgaggcagg tttgttttct tgacgcgaat tgatgtatga gattaaccta
       61 gcttcagata cgaaaaaacc gccctgcaag gcggcttttt cgaaggatca gagacctgca
      121 agtttctgaa ccgtggtaac agggtgtaca agaccgctgc aaccaaatct gtcctttcag
      181 tgtagccgta gtcaggcttc aacttcaaga actctgcacc agtaatctct tgtacacccc
      241 tttaccagtg gctgctgcca gtggcgcttt tgcgcgtctt tccggattgg actcaagatg
      301 atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
      361 cttggagcga actgcctacc cggagctgag tgtcaggcgt ggaatgagat aaacgcggcc
      421 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
      481 catcaggggg aaacgcctga tatctttata gtcctgtcgg gtttcgccac cactgatttg
      541 agcgtcagat tttgtgatgc ttgtcagggg ggcggagcct atggaaaaaa cggccgtcgc
      601 tcggccttac actttctctc ttacgttacc gctcggtttt ctcgagttct tacacgcatg
      661 cccttgccgc tcgccgcagc cgaacggcag gagcgtagcg actgagtgag cgaggaagcg
      721 gaaaagagaa catccctaca ttgagcactg acgctctgag gcctcttatg acgaaacaac
      781 agaaaacagc gctgaacatg gctaaattca tccagaatca gtctctgctc ctgctggaga
      841 agcttaacga gcttgatctc gatgtcgagg ccgacctttg tgagaaactc cacgatgatg
      901 cggagcatct tttccgtaca ctgtcctcga gactggatgc attacaggat ggtaactgat
      961 ggccagagtg tctattagtg aggctgcccg actgacgggt aagagtagaa caacactgca
     1021 caggttaatt aaagcaggtg acctgtccac ctgtagcggt gaacgaaatg caaaaatggt
     1081 agatacttca gagcttttac gtgtctttgg tccctttgaa caaccgaagg gtgaacacca
     1141 ttctggacag gtctctgaac agcgtgatac agcctctgct gaccagagtg aacaggtgat
     1201 tcgtcatctg aaacaggaga ttgaacacct gaatacctta cttttgtcca aggattcgca
     1261 cattgacagc ctgaaacagg ccatgctcct tcttgaaaat caccaggcaa aattaactga
     1321 atccccgtcg ccatggtgga aattctggaa aaaatcctga ccccgtcatg cttcagtttt
     1381 cctctccggc catcccgggg agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg
     1441 ctcctcacca ctaaaaatac ccacacccca cgcaaaatta aaattttgaa gataactcat
     1501 taatatcagt tagttgtggg ttattacatt acgcagtatt actttctgta aggtgaatcc
     1561 aagtcgtcgg cgattcgtgt acgacttgcc gacgactcgt catagagtca tcgacgactc
     1621 acttaagact atcagtaact tatgacgact tgactctaga gtcgaatcgt cattttggtg
     1681 tttttttgac cttgacgact ctagagtcaa gtcacgagtc gaatcgccgg tgaatcgtgt
     1741 ggtgagtcgt ccggcgactt gccataacgt gattgccggc cgtcgacgaa cccgattcct
     1801 gatcgtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa
     1861 ttgcgtcccc gttcaccgtt ttcggatagg aagaacgccg gaaacaggac ttaccctggc
     1921 tgaatggcgt gaatttttgc gctatgcact tgcgtgcata ctcatgcatg ccgtaaaaac
     1981 agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc
     2041 gtcggaaacg ccctgaggcc gttttagcgg tgcgtacaat gggacgttat ggtaaatcaa
     2101 cggcggatgc ctgagtctgt gttagagtca gcggattgtg ataatcagtg actcagggag
     2161 atgttttggc tactgacgct caggtaaaag aaattaatgc actcatcaaa aaatacccgg
     2221 actcatgctc catctgtcat gaagtctatg atgaggatga tgtgacctac accgtttttg
     2281 ggtatgaccg gaaaggtaaa atacaggtca ccacgggatg ctgtgccgga atgctgacag
     2341 agccggttct gctgggtgtc tgtgggtgct ttgacccgga agaacgtgac gagatcatgc
     2401 agaatcaccc catggcaaag cagtttttca cagagtgatt ccgtatctgt tgagtctgaa
     2461 gcccgccttg tgcgggcttt ttcatatctg cgtgaaatta ggcgttatgg taaatgctgt
     2521 gaacagaata gttgcctccc cctttttagg gggggaggct tttggtggag acaatgggat
     2581 ttgaacccac aaccatcaga ttagaagtcc gctgctctac cgttaaagct atatccccat
     2641 ttggacccaa caaagtcggg accgtctttg atttaacatc attgcattat ttttgtgaag
     2701 ttttttatgt ggttatcggt ggtggctgaa cagggtatgt gatgagacgt tattataact
     2761 ttagaaaaca gacagatgca aaaaggcgcc ctaaagggcg ccttttttta atagggagcg
     2821 ggactcgaac ccgcgaaacg caaagcgttt ggtcgcttat taggcgactg ccttcaccaa
     2881 ctcggccatc cctccactgt atgggggatg accattattg attgtcggaa agatgatgtc
     2941 catgaaaaaa agtaacaatc atgcattctt gttgacgttt ttaccaacct acatctatga
     3001 ggcaaggctt taattttact gagccctttc catttttaag aagtattcaa aaagcatttt
     3061 ctttatagag cgatcattta tttctttaac atgacggatc aagtcttctg gttttttaat
     3121 aattaaatcc acttttgggt gcgttttttt aaaatgctct attctgattt cagattgctc
     3181 tgttaaaccc cctgtaatcc agagttcgaa ggattttttt agttttttgt aatcaggatg
     3241 tgatttacaa taatttataa gtctaggtat ccttacatct agccattgat ctacttgttt
     3301 atcatcaaga ttactttctc tttttattcc tttgcattca atgaaggata tcgatacttc
     3361 atcccttatc gatgtgatat ctgcctctgc cctttcattc tgtgtggtta tgtactccct
     3421 tcctaatatc aatctggatg ttggctctat gcgccttttg atatctgagc aaatgaactc
     3481 aaagagtcta ccttttagat tttgagttat gccgtttctt tcatttagct ctttggttaa
     3541 tctagctaac tctcctgata agttttcatc atgccattta tttcccaata tctcgcttat
     3601 tttatcaatt gcggttatat ttctttttcc aaaaatatta ttatatgtta tagccatcac
     3661 accctttgat tttgcaaggt acaaagcttc ttcagtgtaa ccatttgata tgtatacaaa
     3721 tatacagttt ccgatgttct tcattgatat cgtcatatcc atttttttta taaaaatgga
     3781 tatatcgtca agggttgtga ttggctgtgt gtttaagtcg caaacaacga atcctggttt
     3841 cgttttgtta tcgtattttt ttgttagagg gtgagtatag gatggtccag ttatatgcca
     3901 ttcaaatgtg ccaacgcgag gatggttgtt atcatcaccc attgccttta cttttccaaa
     3961 actaactaaa ccaagctgct ttaaccaggt cctggattgc tctatcatta tcccagaaat
     4021 caattctttt ttgttttgcc cttctagtag aaaatgttca cttccctcaa actcttttaa
     4081 gtataaatat ctgtctccgc tagcgcttga tatttctaat attatttctg attttattag
     4141 tgaggatatt attgattcgt aaggtatatg atttttctgc cgaaaagggg agccgcataa
     4201 aataccaaac tcatctcttt ttatcattcc tcctctgctt tttatgcatc tgatagccat
     4261 ataataagag gagttttctt tcctcaatga ggagtataga gcattccagt atttttctga
     4321 cgcatagtcc tttttcagat aaataaatct agccctgtgg gggaatatta tgtaagagag
     4381 ttttctaatt ttattagtcc ccctagatat ccttttcctc gctgctgccg gtgtaaaacc
     4441 aaaatttttt accaatgttt cggcaagttc ggaggttagg caagggccct gttcattcaa
     4501 aatattatca aggtttattc tggtcatttt ttgctcacgt cacactacgc tatttataat
     4561 ctgcactatc gtgcaggcta gcagaagcga aaaaaaattc tagcgatggc gcatagagac
     4621 tgggttttag aggggggatt ctggactttc tacaaaaaac gtcacaatag gatttttttc
     4681 ctgta
//
