LOCUS       NZ_CP031822             3302 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF235 isolate INF235 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP031822
VERSION     NZ_CP031822.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357198
            Assembly: GCF_003590665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Distinct evolutionary dynamics of horizontal gene transfer in drug
            resistant and virulent clones of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Department of Biochemistry and Molecular
            Biology; Bio21 Molecular Science and Biotechnology Institute,
            University of Melbourne, University of Melbourne, Parkville, VIC
            3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 119x
            Sequencing Technology :: Nanopore minION; Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:32:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,453
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 77 of 193
            Pseudo Genes (incomplete)         :: 119 of 193
            Pseudo Genes (internal stop)      :: 48 of 193
            Pseudo Genes (multiple problems)  :: 41 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031822.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF235"
                     /isolate="INF235"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-11"
     gene            1104..2636
                     /locus_tag="D0898_RS28045"
                     /old_locus_tag="D0898_28110"
     CDS             1104..2636
                     /locus_tag="D0898_RS28045"
                     /old_locus_tag="D0898_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_064161748.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRSVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPTINSYYKINNLEKKNIHHYLWVTGNVYNKDLKRLDSFKS
                     NNKNIDVDYLFGEHLDDFFYKKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     Typing          complement(2685..3165)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.687"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagtct
       61 ggttttacat tgagcactga cgctccgagg ccccttatga ctaaacaaca gaagaccgcg
      121 ctgaatatgg caaaatatat ccagagccag tccctgtttt cctgtccggc catcccgggg
      181 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca cgaaaaatac
      241 ccacacacta cgcaaaatta aaattttgca gatttttatt taatatcagt ggtttacctt
      301 cctgaaattt cgttttttaa attttattta tatagatccg actctatgac gactcgtcat
      361 agagtccccg gcgagtcgtt atagagtcgg taacgactca gaactataaa aacaataagt
      421 taataacgac ccgactctag acccgactcc ccgtttggtt gttttttaga cgcggcgact
      481 ctgggtacga ctctagtgaa gagtcgccgg tgaatcgtgt ggtgagtcgt ccggcgactt
      541 gccataacgt gattgccggc cgtcgacgaa ccggattcct gaacgtgctg aaaaccggtt
      601 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
      661 ttcggagtag aagaacgccg gaaacaggac ttaccttggc tgaatggtgt caatttccgc
      721 gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgtg cgtttctggc
      781 gggttttcgg gtggtttgtt gcctgttttg ccggtttccc gtcagaaacg ctctgagggc
      841 ctctcaggcg gtgctgataa cggatgttat ggtaaatgga gtagctgata gttcgataaa
      901 aaaatgtcac aaaactatct ttatgattta aaaggattaa ataattataa agacgatatt
      961 tctttgtgct taatttaaag taagtagtta accatatgat tttaaattgt tttttatagt
     1021 cacttttttt taaaaagact gcgcgccatt acacgtagtg taatgttata aatagcgtag
     1081 tgtgacagaa ataaggtgag gctatgaaaa aaagtgttgt tagacaagtg cttgaaatga
     1141 gcgggccatg catctcttca gatcttgcag agagaattca gtggcaacat ccctcaatgt
     1201 cccctgaagc gatacgtaaa atgatctcgc gaagcacgga cataggtaag ctgccatttt
     1261 tgaaattcag ccataacagg agattcattt atctaaaaga tgattttggc agtttcaaat
     1321 tttggcgagc tctcgaaaag tgcatgtacg aagcaaactc aacttactca cacgcgatac
     1381 ttgccgtaat aaataatggc ggttatttga aggtgaaaga ttttgggatt gtgtctggat
     1441 ctcctataaa acaagctaaa catttaagtt atgaaacagt tctaagaaat ctactttcgg
     1501 caaaaatact tagatctgtg tatattgatg gagttggtga ttgcgtactg ataaataaca
     1561 acatagccaa cgatgttaat attagaacaa tggcaaattg cgaatccttt tttgacaaac
     1621 caatattgga gcttgttaaa gcttggttga ggaatttagg cctggtcgct tttaatcaga
     1681 taaaaacaaa atacgatggg gagggaaatc cagttgtagg ctcttttgaa tgggatatga
     1741 ccgctccaag ttacgtttcc cctctcgctg aatatgtcgg cggaaaactg atgcctggtt
     1801 tcgttgcctg tgatttttct ctcggcttta atcgtgatga aattactacc gctgcagcag
     1861 aaacattcat aagaaaagtc cagatgacta agtcttctcg agcgagtcag aggataatgt
     1921 ttgttatttt cgcaaggcgt ttcggcaaaa tagccttcaa taagttgaga agtgaggggg
     1981 ttctggctgt aactatagca aatgcgtttg gtaataaagt tgatgaaagc ttaaccaggt
     2041 tgtcgagggt ggttcagggt tctttgtcca ttgaaaagca tccagatgag ttgcttcaga
     2101 tggttaaaga tctggagtct gtttctggag agaatggcaa tttgagaggg tatatttttg
     2161 agctttttgt atcctcgcaa atttctaatt tttatggtct tggtaatatt tacattaata
     2221 gagagtataa gataaatggt aagcatgcag aagctgatgt tgtattagag agtagtgacg
     2281 atatttatat aatagagtgc aaaaatgtta aggtcttacc ttcaactgaa cttacgcgtt
     2341 ggatgaaaga aagggttcca actataaact cttactataa aataaataat ttagaaaaaa
     2401 agaatatcca ccattatttg tgggttacag gtaatgtgta caacaaagat ttaaaaaggt
     2461 tagactcttt taagagtaat aataagaata tcgatgttga ttatttgttt ggtgagcatt
     2521 tggatgattt cttttacaag aaaaagcggg tgttcaactt atatagaaaa gtcatttcac
     2581 ctgattataa aaaaggcgta atgcctgatt ttgagttaga ggacgatttt ttctagctac
     2641 tgcgtttctt gacgatggcg ctttgaaagg cgtatcttcc atctctgaaa cgaaaaaacc
     2701 acccggcaag gtggtttttc gaaggttcag gaaaccggca agtttctgaa ccgtggtaac
     2761 tggcttaaac ggagcgcagt gaccaaatct gtccttccag tgtagccgta gtcaggtcaa
     2821 cacttcaaga actctgtgta tgcttgtaca cgcctctccg ctccgtaaac cagataccaa
     2881 tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt ggactcaagt cgatagttac
     2941 cggaacaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     3001 gaactgcctt cccggaactg agtgtcaggc gtggaatgag ataaacgcgg ccataacagc
     3061 ggagtgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgcctatc
     3121 agaaatgata ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagtgtcag
     3181 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggccgctg tgcggccttt
     3241 ccttttccac atcacgcgac cgctcggttt tctcgagcgt ttctacgcac gctcctgccg
     3301 ct
//
