LOCUS       NZ_CP031807             9294 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain MSB1_8A isolate MSB1_8A plasmid
            unnamed7, complete sequence.
ACCESSION   NZ_CP031807
VERSION     NZ_CP031807.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357466
            Assembly: GCF_003432165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Distinct evolutionary dynamics of horizontal gene transfer in drug
            resistant and virulent clones of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Department of Biochemistry and Molecular
            Biology; Bio21 Molecular Science and Biotechnology Institute,
            University of Melbourne, University of Melbourne, Parkville, VIC
            3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 119x
            Sequencing Technology             :: PacBio; Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:48:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,112
            CDSs (total)                      :: 5,983
            Genes (coding)                    :: 5,830
            CDSs (with protein)               :: 5,830
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 74 of 153
            Pseudo Genes (incomplete)         :: 82 of 153
            Pseudo Genes (internal stop)      :: 27 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031807.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MSB1_8A"
                     /isolate="MSB1_8A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            complement(join(9213..9294,1..182))
                     /locus_tag="D0887_RS31410"
                     /old_locus_tag="D0887_31410"
     CDS             complement(join(9213..9294,1..182))
                     /locus_tag="D0887_RS31410"
                     /old_locus_tag="D0887_31410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(488..907)
                     /locus_tag="D0887_RS31415"
                     /old_locus_tag="D0887_31415"
     CDS             complement(488..907)
                     /locus_tag="D0887_RS31415"
                     /old_locus_tag="D0887_31415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1310..2995
                     /locus_tag="D0887_RS31425"
                     /old_locus_tag="D0887_31425"
     CDS             1310..2995
                     /locus_tag="D0887_RS31425"
                     /old_locus_tag="D0887_31425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3005..3262
                     /locus_tag="D0887_RS31430"
                     /old_locus_tag="D0887_31430"
     CDS             3005..3262
                     /locus_tag="D0887_RS31430"
                     /old_locus_tag="D0887_31430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            3347..3496
                     /locus_tag="D0887_RS31435"
                     /old_locus_tag="D0887_31435"
     CDS             3347..3496
                     /locus_tag="D0887_RS31435"
                     /old_locus_tag="D0887_31435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4977..6941
                     /locus_tag="D0887_RS31445"
                     /old_locus_tag="D0887_31445"
     CDS             4977..6941
                     /locus_tag="D0887_RS31445"
                     /old_locus_tag="D0887_31445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            6941..7672
                     /locus_tag="D0887_RS31450"
                     /old_locus_tag="D0887_31450"
     CDS             6941..7672
                     /locus_tag="D0887_RS31450"
                     /old_locus_tag="D0887_31450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            7679..8209
                     /locus_tag="D0887_RS31455"
                     /old_locus_tag="D0887_31455"
     CDS             7679..8209
                     /locus_tag="D0887_RS31455"
                     /old_locus_tag="D0887_31455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8237..8416)
                     /locus_tag="D0887_RS31460"
                     /old_locus_tag="D0887_31460"
     CDS             complement(8237..8416)
                     /locus_tag="D0887_RS31460"
                     /old_locus_tag="D0887_31460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8485..8862
                     /locus_tag="D0887_RS31465"
                     /old_locus_tag="D0887_31465"
     CDS             8485..8862
                     /locus_tag="D0887_RS31465"
                     /old_locus_tag="D0887_31465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(8924..9223)
                     /locus_tag="D0887_RS31470"
                     /old_locus_tag="D0887_31470"
     CDS             complement(8924..9223)
                     /locus_tag="D0887_RS31470"
                     /old_locus_tag="D0887_31470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             1..7995
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3600..4178)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6942..7669
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gcacgggcgg cggtcacttt ggcgcgaacc tgatcttcat actctgcctg ggccagttct
       61 tcgttagcca gtcgctgcaa cagcgcagct ttgtccgggc caccttccgg cagtatgcgg
      121 ggcaggcttt cagccagttc ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc
      181 attgcggagc ctcccgggtt aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg
      241 aaagaatatg gtcgtgtcag tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac
      301 ggcatccatt ttacgtcaaa acatatgcca tgcagttgcg tgcataggca tgcattagga
      361 taaaatttat cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct gttttatccc
      421 gtagccgccg gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg cgttatggta
      481 aatccactta ctgtctgcct tcatagccat cgagataaac agctgaaatg gtgtcagcca
      541 gtagccttga ttttttcggt gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg
      601 ttgtgccttg gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca
      661 catcactccg tgattttccg gtcgctttat cctttgcgcc catatcccag aactgccgga
      721 aaaaaggcac catcattcgt gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac
      781 cgtcatctga agcatatacg taatgccggg tatgctgttc cacgctgccc tttggagcac
      841 aggcagtgag agttgctgtc gctgttatca tggcgataag acacagtttg ctgccggaac
      901 gtctcatgag cgtctcctta cctttaattc ttttttctca ttgagttata tatccgcagg
      961 ctatcttatc atcagtttct gaccgtgttc agtgaccgaa caacccgaac cagacccccc
     1021 gtttcttttc ctgtcccgga gggggctttc tggtatctca tataatgatt tcagtaaaac
     1081 atactgaggc cggagaacgg cgccgtttgt gctggttttt gttgcatctg gaggggattg
     1141 cgcatggtgt gagtggctgt ttttttgatc attttcacaa aaacgcaaat ttacagactt
     1201 gacatgaaaa acaaggcagc gtaattttta tactgtatat aaaaccagtg gttatatgta
     1261 cagtatttta ttttagttta ttgttttaaa agtcaaagag gaaaagtata tgagtggcgg
     1321 agacggacga ggtccgggta attcaggtct gggacacaat ggtggtcagg ccagtgggaa
     1381 tgtgaacggt acatcaggta aaggtggccc ttcatcaggt ggtggtacgg atccaaacag
     1441 cgggccgggc tggggtacga cgcatacacc taacggggat attcataact ataacccggg
     1501 ggagtttggt aacggcggga gtaaacccgg tggtaatggc ggtaacagcg gcaatcatag
     1561 cgggagctct ggtggcggac agtcttcggc caccgcgatg gccttcggtc tgcctgcctt
     1621 ggccactccg ggggctgaag gactggcttt atccgtttcc ggcgatgcgt tgtcggccgc
     1681 tgttgctgat gtgctggctg ccctgaaagg tccgtttaag tttggtctgt gggggattgc
     1741 gatttacgga gtgctgcctt ctgagatagc aaaagatgat ccgaaaatga tgtcaaaaat
     1801 cgtgacgtca ttgccggcag acacggtaac ggagacgccg gtcagctccc tgccgctgga
     1861 ccaggcgacg gtcagcgtca ctaaacgtgt ggcggatatt gtgaaggacg agcggcagca
     1921 tattgcggtt gtcaccggcc ggccaatgag tgttcctgtg gtggatgcga aaccgacaaa
     1981 acgtccgggg gtattcagtg tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc
     2041 taaaggtgtt ccggccgcga aagccccgcc aaaaggcatt attgctgaaa aaggtgattc
     2101 acgtccggct ggttttacgg ccggtggtaa ctcccgtgaa gccgttattc gtttcccgaa
     2161 agagagcgga cagaaaccgg tctatgtgtc ggtgacggat gttcttactc cggcacaggt
     2221 aaaacagcgt ctggaggaag aaaagcgtcg ccagcaggca tgggacgctg ctcacccgga
     2281 agaggggctg aaaagagagt atgataaagc gaaagctgag ctggatgctg aagataaaaa
     2341 tattgcgacc ttaaacagtc gcattgcatc gacagagaag gcgatccccg gtgcaagggc
     2401 tgctgttcag gaagccgata aaaaggtaaa agaggcagag gcaaataaag atgattttgt
     2461 gacttataac ccgcctcatg aatatggctc cgggtggcag gatcaggttc gctatcttga
     2521 taaggatatt cagaatcaga atgagaaatt aaaagctgcc cagacatctt taaacgaaat
     2581 gaatgaatcc ttatccaggg ataaggctgc gctttccggg gcgatggaga gccggaaaca
     2641 aaaggagaaa aaagcgaagg atgcagaaaa taaattaaat gaggaaaaga aaaaacctcg
     2701 taagggaact aaagattacg gccatgatta ttttcctgat cccaagactg aagatattaa
     2761 ggggttggga gagttgaaag agggtaaacc taaaacccct aaacaaggtg gtggtggtaa
     2821 gcgggcccga tggtatggtg ataaaaagcg taaaatttat gaatgggatt cccagcacgg
     2881 tgagcttgaa gggtaccgcg ccagtgatgg cgaacacctc ggggcattcg atccaaaaac
     2941 gggtaagcag gttaaagggc cggatccaaa acgaaacatt aaaaaatatc tttaagaggt
     3001 aaatatgggg cttaaattac atattcattg gtttgataag aaaaccgaag agtttaaagg
     3061 tggtgaatac tcaaaagact tcggtgatga tggttctgtc attgaaagtc tggggatgcc
     3121 tttaaaggat aatattaata atggttggtt tgatgttgaa aaatcatggg tttcgatatt
     3181 acagccacac tttaaaaatg taatcgatat tagtaaattt gattactttg tatcctttgt
     3241 ttaccgggat ggtaactggt aagcaaaagt tatcaaggat gatttgaaat acagacttcc
     3301 ggtgttcagg gatgaatacc ggggttttta aatacaggga aggtatatga aaaaggtaaa
     3361 ggttgttttt ctttttatat tgattttttc aggctttctt ctggtcgcat gtcaggcaaa
     3421 ctatatccgg gatgttcagg gtggaacggt ggcaccatcg tcctcctctg aactgacggg
     3481 gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3541 atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc
     3601 agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt
     3661 ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac
     3721 aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttt
     3781 ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacggggg
     3841 gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagagtg
     3901 tgagctatga gaaagcgcca cacttcccgc aagggagaaa ggcggaacag gtatccggga
     3961 aacggcaggg ttggaacagg agagcgcaag agggagcgat tcatcggaaa cggtgatgat
     4021 ctttaagtcc tgtcgggttt cgccactcct gactgattca tggttgagcc accggctccc
     4081 acagatgcac cgaaaaagcg tctgtttatg tgaactcagt caggagggcg gagcctatgg
     4141 aaaaacgcca cctgcgtggc cttgccgttt tgcctcacat gttagtcccc tgcttattca
     4201 cggaatctgt gggtaacttt gcatgtgtcc gcagcgcccg ccgcagtctc acggccggag
     4261 cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctacaaga cggacaccgg
     4321 acgcgctgat gtggcatatt gtcccctgat taagtcttta tattccctga taataaatta
     4381 ccaggatagg tgaatcatat cggtttttcg ctgaacattt tacgttaacc tatctctgtt
     4441 gcttttttct ccttacggag aaatacaggt catccccgcg agggcgtggc tgacctcagg
     4501 gggatatccc ctgcttacgc tggctggacg ccaccgacgc acggtctctc cccctctttc
     4561 tggctccgtc cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca
     4621 cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tggcgaagca gcaaagctgc
     4681 tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac
     4741 cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt
     4801 tcctccgggg ctgcgccttt tccgcatggg gcggaaagag gctggcctcc gtggtgaaag
     4861 ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc cttggggcat gtccgcggct
     4921 tgtgcagcgg gggctgcaca tcacgccggc aaaccatcgt ctgaaggaga gagaacatgt
     4981 ttaatacgga ttcgcttgcc tggaagtggc acattccgga gctggcccgg cagatttatc
     5041 cgtgggtcag ctggctgtac caggagtatt acggtctggc cggggctttc ctagttatcg
     5101 gcctgttttt cggtctggct gaactttggg ccggacggat gcgttttgtg gggctgagtc
     5161 ataaggagcg acgggagtat gccgatcgct ataagcgcga taataaacgg ccagcgccat
     5221 tttcggtgct ggcctcgctg gattcgcccg ggcggatggt cagggcacgt cgacatgtct
     5281 ggaactacgc gtactggctg accggcgcgg ccggtctggc ccttctgggg gggctggttg
     5341 tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg tccggacctc tacgcaaacg
     5401 cagattatta cctgctgcag ccggctaacc tgttcgggat tgcctggtgc gcgggcgggc
     5461 tggctgtcgg gattgtgttg gcgcggctgc tggcgttcct gctgcttgac ggtcattttg
     5521 cggctgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc
     5581 ggacaggaga gatgaccgac gtgcgccacc tgcatttcgg ggagccggta ccggttaatg
     5641 cgctggccga tttttcctcg gagcaggccc ggcaggagca ggcggtgttt ctgggtaagg
     5701 atgagcaggg caagccggtg ctggttccgc gtgatacctg gcgcaagacc aacattcaga
     5761 tcctcggcct gcccgggagc ggcaagagcg tgatggccac caacgcgctc attcgctgcg
     5821 tacgcgattt cggggacgcg gtggtgtact tcgaccccaa aggggacgca tgggcgccgc
     5881 acgtcttccg ggcgcactgt ccggacttca ccctgctgga tctgcggccg gggaaaccgg
     5941 cgcagctgaa tctgttccgc gatctcgacc agtacgcgct gaaaaacctg ctgattgccg
     6001 ggcttaacct gggcgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag
     6061 cggcgaagct gattgcggag cagtttccgc agggggcaaa cattcagcag gtgctggcgg
     6121 cggcgtacgc gctgccggag gccctgaaaa aggacgtgaa ggggttgatc actaggctgg
     6181 aaaacgtcgc tgacctgagc gtcctgcaga cggacagtgg catcgatgtg gcggggatta
     6241 tcaacggcgg cggctgcctg tacgttatcg gctcgatgga cgacgaggcg gtgatccgtg
     6301 tgcaaaaaat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggac gagttccggg
     6361 agtggccaca cgtcagcgtg atgctcgatg aaattaaata tctgctgtcg aagtatgtac
     6421 tgaacgcact ggggacgctg cgcagccggg actgcaatct gttgctcgcg caccagtcgc
     6481 tgggcgattt cgggcagtgc gggcaggatc tgcccgctga atttgtgaaa accacggtac
     6541 tggataacac gccgatccgc tggttttacc gggcggccag cctggagtcc gcgcagtggg
     6601 cggcggggca gaccggggaa atcagggtgg acgtggagcg ccgccgtgcc agccgggagg
     6661 cggggaacgt ggagcatatc agcggggaca gcttcatcca gaaggaggcg cggccgctgt
     6721 ttgacgtgaa caccctgcaa cacctgccgg acgggttcgc ggtcatgacc gggctgggtg
     6781 tggcccggat ggcgttcagc agtccgctgc gggttgagcg ccgggaaatc ccgttgcagc
     6841 gtttcccggt gctggcgaaa accgatcccc tggcggagtt ccggcccccg gccgacggcg
     6901 gcgggccgaa agcggatgat gattttgcgg ggatttactg atggcgctgg agctgtacaa
     6961 cgtcagccac gcgaaacgac aggcgcggaa cgcggagaaa acccggctga ccctgcgctg
     7021 gttgcgggaa gagctgtgtt ccacggcgga gctggttgcg cggcggctgg ggattgcggc
     7081 ggtgcagccg gtctaccgct tcctcgacag cctggtggcg aaagggctgc tggtccggac
     7141 caaatatccg gtggacggcc gtcaggtcag cgtgtggggg ctgacgccgc atggcgtggc
     7201 gttctcgttc gacgaggacg aaccgctggc ggatgttatt ccgtttcagc cgtcaagggt
     7261 gtcggcagca catctgccgc accggctggc cgtgcaatcc ctgcgccttg atatggaagc
     7321 cagaggggcc accggctggc gcattctgca ccgtctgagt cttaaaggca tgaaagtgcc
     7381 ggacgccctg gcggagctgg acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa
     7441 gagccgtcgc cggtatcagg aagtggtgtc cgggtatctg ttcaaccgca aagctaacgg
     7501 catcgatgaa atctggtaca tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc
     7561 aatcctgtcg gtggatgaaa tcgtgaaccc acagaccggc gaggcccgca aaacggcaga
     7621 gctggatcgt gaacggttgt ttgcgtgctt taaatttatg acaacggagt gagggattat
     7681 ggcgctgtac atggtattta ttgaagacgc ggaggaccgg aacggcgagc gctgcatcat
     7741 gccgaccagc gagcatctgg aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt
     7801 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     7861 cgagcggctc aacagcgacg aaaaagccac gtactttttc actggtaaaa atccgcaatg
     7921 ggaacggaac aaacccacag gaatgaagct gtgtgattac ctctatcgca aggtggagcg
     7981 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     8041 taacgcgatg attgacgtgg tgagtgagcg gcacggggaa agcgcggtga acgagaagct
     8101 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tacctgaact
     8161 gccggaggac aaacctgccc gccggtggtg gccgttcggc aaaaaataaa tgccagcacc
     8221 ggccatgccg gtgctgttat tcctccccga gcttatcccg catcacctgc cagagtgttt
     8281 ccgcatcctc gtgtagccgc tcgcacatgt cggccggggt atccatatcc agctcattca
     8341 gtttatcgag caatagcagc gactgctcct taataaattt agccatattg agggctgttt
     8401 tttcctgttt tgtcatgatt ctttccttcc gggcatggtg tgcgcctgtc aggtatactg
     8461 tctcaaaaat agcggggtga gacaatggcg tgggtgacgg tgagacaggc gacggaactg
     8521 acgggtaaag cccgaagttc cctgtatcgg gatatggcga aaggccgcgt atcataccgt
     8581 acggaagctg atggcggccg ggtggtggat accagcgagc ttatccgggt gtacggtgag
     8641 ttaagacagg atgagactca cgagcgggac gagttgagac tgtcgggtga gactgaaaaa
     8701 atggcctccg acgcgttgat cgcggagata aaagcactca gggaggaggt ggccgggcta
     8761 cggcaggaaa tgcagtccat gagactgctg gaacacaaac cggagacagc agagaccccg
     8821 gccggaacac cgcgccggtg gtggccgtgg ggaaaacgct gacgcgctgt gcttgtccaa
     8881 ccggcgacgg ccacgcaatg aattgcgtcc ccgtcaccgg ttattagaga acaatatggg
     8941 gattctggcg ctggccgacc acggccagaa cccggacttt tctggtctgg tcatcaacat
     9001 catagaggac atgctgtccc atcagactga tccggcgaac gtcctcgcca tagcgtgagt
     9061 ccacttcata gagtcgcggg aagtcggcca gtttgtccgc cgcagcgaga acccggtcgg
     9121 taacgttctg cgcatcccac agggatgaat tttccgcgat atagtcaaac agcgtcgtta
     9181 catcctcgat ggctccctgc ttccattcca cgtcatacgg catcgcgcag ttcctgataa
     9241 cggccatgca ggcgctggcg gagttgttcc gtggtcatac ccggacgcgt gtca
//
