LOCUS       NZ_CP031758             2938 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella aerogenes strain FDAARGOS_327 plasmid unnamed2.
ACCESSION   NZ_CP031758
VERSION     NZ_CP031758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173340
            Assembly: GCF_003546885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.,
            Aluvathingal,J., Geyer,C., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.,
            Aluvathingal,J., Geyer,C., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3.0
            Genome Coverage                   :: 722.453x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:49:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,197
            CDSs (total)                      :: 5,074
            Genes (coding)                    :: 5,006
            CDSs (with protein)               :: 5,006
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 33 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_327"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_327"
                     /db_xref="taxon:548"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: DC"
                     /collection_date="2015-01-11"
                     /collected_by="Children's National Hospital"
     gene            <1..752
                     /locus_tag="CEQ05_RS27010"
                     /old_locus_tag="CEQ05_27010"
     CDS             <1..752
                     /locus_tag="CEQ05_RS27010"
                     /old_locus_tag="CEQ05_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_192574433.1"
                     /translation="RKVQMTKSSRTNQRIMFVLFARRFEKNAFNKLRSEGVLAITIANA
                     FGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESISGENGNLRGYIFELFVSSQI
                     CNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVECKNVKTLPSMEVGLWMKTRVP
                     IINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDEFKCNNKKIDVDYLFGQSLDDFFY
                     QKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(957..1115)
                     /locus_tag="CEQ05_RS27495"
                     /old_locus_tag="CEQ05_27015"
     CDS             complement(957..1115)
                     /locus_tag="CEQ05_RS27495"
                     /old_locus_tag="CEQ05_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(1611..1805)
                     /locus_tag="CEQ05_RS27025"
                     /old_locus_tag="CEQ05_27025"
     CDS             complement(1611..1805)
                     /locus_tag="CEQ05_RS27025"
                     /old_locus_tag="CEQ05_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2089..2412
                     /gene="mobA"
                     /locus_tag="CEQ05_RS27030"
                     /old_locus_tag="CEQ05_27030"
     CDS             2089..2412
                     /gene="mobA"
                     /locus_tag="CEQ05_RS27030"
                     /old_locus_tag="CEQ05_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001699352.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          1841..2080
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(1048..1568)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.905"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2090..2409
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="2.4899999999999998e-62"
ORIGIN
        1 taagaaaagt tcagatgacc aagtcctcta ggacaaacca gaggattatg tttgttcttt
       61 ttgctagacg ttttgagaag aatgccttca acaagctaag gagtgaaggg gttctggcta
      121 taactatagc taatgcgttt ggtaataagg tcgatgagag cttggccaga ctatcgaagg
      181 tgattcaggg aactttatct attgaaagac atcctgatga gttacttcaa atggtaaaag
      241 atctggagtc tatctctgga gagaatggaa atttaagggg gtatattttt gagctttttg
      301 tttcctctca aatttgtaat ttctatggtt atggaaatgt tcgtattaat aaagaatata
      361 aaataaacgg taaacatgcc gaagctgatg ttgtattgga aactaacgat gatatttata
      421 tcgttgagtg caaaaatgtt aagactttac cttcgatgga ggttggtctt tggatgaaaa
      481 caagagtgcc aattataaat tcctactaca aatcaaataa ccctgataac aaaaatatac
      541 atcatcgttt gtgggttacg gggaatatat atcctaaaga tataaaccga ttggacgagt
      601 ttaaatgtaa taataagaag attgatgttg attatttatt tggtcagtct ttggatgatt
      661 ttttctacca aaaaaaacaa gtgtttaatt tatataggaa agtcatttcg ccagattata
      721 aaaaagggca aatacccgat tttgacttat agaatgacgt tttctagtct ctgcatttct
      781 tgacgatgac ggcttgaaaa gcgtatcttc ctccatgaaa cgaaaaaacc acccggcaag
      841 gtggtttttc gaaggttagt taatcctggc agattaccta acctaggtaa ctggcttcag
      901 cagagcgcag ataccaaatc ctgtccttta agtgtagccg tagttggggc atcacttcaa
      961 gaactctgta gtacggaccg cattatcatc gctctgctat tccagttacc agtggctgct
     1021 gccagtggcg ataagtcgtg tcttttcggg ttggactcaa gacgatagtt accggataag
     1081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1141 tacaccgggc cgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1201 agaaaggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg cacgagggag
     1261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctggctt
     1321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccaccggc
     1381 gcggcctttt tcgggttctt gttttttgct cacatgtttc ttttggcttt atcccctgat
     1441 tctgtggata accgcattac cgtctttgag tgagctgata ccgctcgccg cagccgaacg
     1501 accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gccttatgtg gcattttctc
     1561 cttacgcacc ggtataccgt tctgcatcct gtctggagca ggatacactg ttataccccg
     1621 gtgccacttt ccgcttcaaa gcgtgtctgt atgctgttct ggagttcttc cgcgagttcg
     1681 tgcagcttct cacacatggc ggcctgttcg tcggcatcga gtgcgtccag cttttcgagc
     1741 agcgtcaggc tctggctttt tatgaatccc gccatgttga gtacggtttg ctgctgcttg
     1801 ttcatctttt cgttttctcc gttctgtctg tcatctgcgt tatgtgatta tatcgcgtac
     1861 cacttttcga ctgttttgct gccgctattc tgccgcctgg ctttttgacg ggcatttctg
     1921 tcagacaaca ctgtcactgc caaaaaactg ccgtgccttt gtcggtaatt cgagcttgct
     1981 gacaggacag gatgtgcaat tgttataccg cgcatacatg cacgctatta caattaccct
     2041 ggtcagggct ttgccccgac acccatgtca gatacggagc catgttttat gacaaaacga
     2101 agtggaagta atacgcgcag gcgggctatc agtcgccctg ttcgtctgac ggcagaagaa
     2161 gaccaggaaa tcagaaaaag ggctgctgaa tgcggcaaga ccgtttccgg ttttttacgg
     2221 gcggcagctc tcggtaagaa agttaactca ctgactgatg atcgggtact gaaagaagtt
     2281 atgagactgg gggcgttaca gaaaaaactc tttatcgacg gcaagcgtat cggggacagg
     2341 gagtatgcgg aggtgctgat cgctattacg gagtatcacc gtgccctgtt atccaggctt
     2401 atggcagatt agcttcccgg ggagaaaact gttgaaaaca gacggtatga acaccgtaag
     2461 ctcccaaagt gatcaccatt cgctttcatg cataactatg cagcgagatg aaaacgatcc
     2521 tgacgcatcc ttcctatttt tccggggtaa aacatctctt tttgcggtgt ctgcgtcaga
     2581 atcgcgttca gcgcgtttca gtggtgcgta caattaaggg attatggtaa atagggcggt
     2641 tgatatatga tgatgaaatg tcacaaacgt aacatcttga tttttaagga taaatctcgc
     2701 aggtgtgttt tttgtgatat gttgcttttg ttaaatgttt gatttttatg atttttttct
     2761 ttgtgttttt ttttgggatc tatagatatt acacgtagtg taattttata aatagcgtag
     2821 tgtgacaaaa ataagatgaa tttatgaaaa aaaacattgt tagacaagtg cttgagagtt
     2881 atggtccgtg catatcttct gaacttgctg agaggattaa gcgtcagaat ccttctat
//
