LOCUS       NZ_CP031718            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCKP020003 plasmid p2_020003, complete
            sequence.
ACCESSION   NZ_CP031718
VERSION     NZ_CP031718.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909161
            Assembly: GCF_002164855.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain SCKP020003
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:03:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,484
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 48 of 127
            Pseudo Genes (incomplete)         :: 80 of 127
            Pseudo Genes (internal stop)      :: 15 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031718.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCKP020003"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_020003"
                     /geo_loc_name="China: Yibin, Sichuan"
                     /collection_date="2016-09"
     gene            complement(join(10020..10060,1..139))
                     /locus_tag="CAY64_RS00135"
                     /old_locus_tag="CAY64_00135"
     CDS             complement(join(10020..10060,1..139))
                     /locus_tag="CAY64_RS00135"
                     /old_locus_tag="CAY64_00135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(165..593)
                     /locus_tag="CAY64_RS00140"
                     /old_locus_tag="CAY64_00140"
     CDS             complement(165..593)
                     /locus_tag="CAY64_RS00140"
                     /old_locus_tag="CAY64_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(757..1071)
                     /locus_tag="CAY64_RS00145"
                     /old_locus_tag="CAY64_00145"
     CDS             complement(757..1071)
                     /locus_tag="CAY64_RS00145"
                     /old_locus_tag="CAY64_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1059..1337)
                     /locus_tag="CAY64_RS00150"
                     /old_locus_tag="CAY64_00150"
     CDS             complement(1059..1337)
                     /locus_tag="CAY64_RS00150"
                     /old_locus_tag="CAY64_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1512..1877)
                     /locus_tag="CAY64_RS30510"
                     /old_locus_tag="CAY64_00155"
     CDS             complement(1512..1877)
                     /locus_tag="CAY64_RS30510"
                     /old_locus_tag="CAY64_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(1874..2245)
                     /gene="tolA"
                     /locus_tag="CAY64_RS00160"
                     /old_locus_tag="CAY64_00160"
     CDS             complement(1874..2245)
                     /gene="tolA"
                     /locus_tag="CAY64_RS00160"
                     /old_locus_tag="CAY64_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2519..2764)
                     /locus_tag="CAY64_RS00165"
                     /old_locus_tag="CAY64_00165"
     CDS             complement(2519..2764)
                     /locus_tag="CAY64_RS00165"
                     /old_locus_tag="CAY64_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3091..4776
                     /locus_tag="CAY64_RS00170"
                     /old_locus_tag="CAY64_00170"
     CDS             3091..4776
                     /locus_tag="CAY64_RS00170"
                     /old_locus_tag="CAY64_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4786..5043
                     /locus_tag="CAY64_RS00175"
                     /old_locus_tag="CAY64_00175"
     CDS             4786..5043
                     /locus_tag="CAY64_RS00175"
                     /old_locus_tag="CAY64_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5128..5277
                     /locus_tag="CAY64_RS00180"
                     /old_locus_tag="CAY64_00180"
     CDS             5128..5277
                     /locus_tag="CAY64_RS00180"
                     /old_locus_tag="CAY64_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6761..8725
                     /locus_tag="CAY64_RS00190"
                     /old_locus_tag="CAY64_00190"
     CDS             6761..8725
                     /locus_tag="CAY64_RS00190"
                     /old_locus_tag="CAY64_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8725..9456
                     /gene="mobC"
                     /locus_tag="CAY64_RS00195"
                     /old_locus_tag="CAY64_00195"
     CDS             8725..9456
                     /gene="mobC"
                     /locus_tag="CAY64_RS00195"
                     /old_locus_tag="CAY64_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9463..9993
                     /locus_tag="CAY64_RS00200"
                     /old_locus_tag="CAY64_00200"
     CDS             9463..9993
                     /locus_tag="CAY64_RS00200"
                     /old_locus_tag="CAY64_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9776
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5381..5959)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8726..9453
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg tccagctcgt
       61 tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata ttgagggcag
      121 ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct aaaccagccg
      181 gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac ttcggcctgg
      241 agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt tttatcttcc
      301 agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc cagaagctgt
      361 tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat ttgtttctca
      421 gggatattca catttccgta tacacggatc agttctgaca catccacgac agggttgttt
      481 ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag cgttcttctg
      541 gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc cactgtatac
      601 ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta tcgctgaatt
      661 ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc aaaccggcga
      721 cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg cccccggata
      781 cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt cccggtcgtg
      841 gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct tatattccag
      901 cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct ggatagcccg
      961 gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga gaaagtcctg
     1021 aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca tggcggagca
     1081 tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc cgcagtaagg
     1141 tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag gtactggctg
     1201 cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc gtgcgctgag
     1261 cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc gagcctgatg
     1321 gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat gactacttta
     1381 atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt cagcttcgct
     1441 tccactaatc agggctgcta caggctcact catcatccag gactattgca gcctgcgggt
     1501 taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt gactggtcgc
     1561 ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc
     1621 gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagg
     1681 tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc aaacgtggcg
     1741 gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg ctgcagtgtg
     1801 gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc cagcactgtc
     1861 agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt tgccaggtat
     1921 tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct gctttacaga
     1981 gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt tgtatggata
     2041 tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc atttcaacag
     2101 cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga gtataaattt
     2161 tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg cacaatgcca
     2221 gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa atgttacatg
     2281 tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat cgtatcgttt
     2341 ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt tacaatttac
     2401 cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg
     2461 gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta aatacccctt
     2521 accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac
     2581 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     2641 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     2701 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     2761 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     2821 atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt
     2881 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt
     2941 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     3001 aacgggagga gtaaggtaat aattatactg tgtatatgta cagtatgttg gctttaattt
     3061 attgttttaa atgtcaaaga ggaaaacggt atgagtggtg gagacggtcg aggtccgggt
     3121 aattcaggtc tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt
     3181 aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg
     3241 acgcatacgc cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg
     3301 agtaaacccg gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggcgga
     3361 cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc tggctactcc gggggctgaa
     3421 ggactggctt tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga tgtgctggct
     3481 gccctgaagg ggccgtttaa gtttggtctg tgggggattg cgatctacgg tgtgctgcct
     3541 tctgagattg caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc
     3601 gatgcggtga cggagactcc ggcaagtact ttaccactgg accaggcgac ggttcgtgtc
     3661 agacaacggg ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcaggt
     3721 cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt
     3781 gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt tccgacagcg
     3841 aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttaca
     3901 gccggtggta actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg
     3961 gtttatgtgt cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa
     4021 gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagac
     4081 tatgataaag cgaaagctga gctggatgcc gaagataaaa atattgcgac cttaaacagc
     4141 cgcattgcat cgacagagaa ggcgctcccc ggtgcaaggg ctgctgttca ggaagccgat
     4201 aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat
     4261 gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag
     4321 aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc cttatccaga
     4381 gataaggctg cacttaccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag
     4441 gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc gcaaggggac taaagactac
     4501 ggccatgatt atcatccagt acctaaaaca gaagacataa agggacttgg agagttaaag
     4561 cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta agcgggcccg atggtatggc
     4621 gataaaaaac gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtatcgt
     4681 gccagtgatg gcgaacacct aggcgcattt gatcccaaaa cgggtaagca ggttaaaggg
     4741 ccagatccaa aacggaacat taaaaaatat ctttgagagg taaatatggg gcttaaatta
     4801 cgtttgacat ggtttgataa gaaaactgaa gagtttaaag gggaagagta ttctaaagac
     4861 tttggtgacg atggttctgt cattgaaagc ttgggaatgc ctttaaagga taatattaat
     4921 aatggtgagt ttgatttgaa gaaagaatgg gttcctttac tacagccatt ttttaaaaat
     4981 caaataaaac ctgatgtgga taattgttat atagcttttg attatcggga cggtaactgg
     5041 taacaaaaaa ttatcaagga tgatttgaaa tactgacttc cggtgttcag ggatgaatgc
     5101 cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa agactatttt tctttttata
     5161 ttgattgttg ctggctttct tctggccgca tgtcaggcaa attatatccg ggatgttcag
     5221 ggcggaacgg tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa
     5281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg
     5341 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     5401 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     5461 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     5521 agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt cccgggttgg actcaagtga
     5581 acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct tacagcccag
     5641 cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg agaaagcgcc
     5701 acacttcccg caagggagaa cggcggacag gtatccggta aacggcaggg tcggaacagg
     5761 agagcgcaag agggagcgac ctaccggaaa cggtggggat ctttaagtcc tgtcgggttt
     5821 cgcccgtact gtcagattca tggttgagcc tcacggctcc cacagatgca ccgaaaaagc
     5881 gtctgtttat gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg
     5941 ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt
     6001 tgtatgtgtc cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagcg
     6061 aggaggcgca atttccccgg cacctgcaag acggtcaccg gacgcgctga tgtgggatat
     6121 tgtcccctga ttaagtcttt ctattccctg acgatcaatt atcaggatag gtgaaccatg
     6181 tcggtttttc gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga
     6241 gaaatgcaag tcatccccgc tggggcgtgg ctgacttcag gggaaaagac cttgcttacg
     6301 ctggctggac gccaccgacg cacggtcttt tcccctcttt ctggctctgt ccggctggac
     6361 gccggccacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa
     6421 atccacggtg ctggacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt
     6481 cagccgctcg ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg
     6541 gtgccgcctt ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt
     6601 ttccgcatgg ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca atcaccacgg
     6661 acgtgaccgc cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcgc
     6721 atcacgccgg taaacccgtc gtctgaagga gagagattgc atgtttaata cggattcgct
     6781 tgcctggcag tggcatatcc cggagctggc ccggcagatt tacccgtggg tcagctggct
     6841 gtaccgggag tattacagtc tggccggttt ttttctggtt atcggcctgt ttttcggtct
     6901 ggctgaactc tgggccggcc gaatgcgttt tgtggggctg agccataagg cgcggcggga
     6961 gtatgccgat cgctataagc gcgataataa ccggactgtg ccattttcgg tgctggcctc
     7021 gctggattca cccgggcgga tggtcagggc acggcgacat gtctggaact acgcgtactg
     7081 gctgaccggc gctgccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga
     7141 gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg aacgcagatt attacctgct
     7201 gcagccggct aacctgttcg ggatagcctg gtgtgccgga gggcttgtcg ccggcgtcct
     7261 tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat tttgcggccg ctgacgaggc
     7321 ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc cagcggaccg gagaaatgac
     7381 agacgtccgc cacctgcatt tcggggagcc ggtaccggtt aacgcgctgg ccgatttttc
     7441 cacggagcag gcccggaaac agcaggcggt gtttctgggt aaggatgagc agggccagcc
     7501 ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt cagatcctcg gcctgcccgg
     7561 gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga
     7621 cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg ccgcacgtct tccgggcgca
     7681 ctgcccggac ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt
     7741 ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt gccgggttta acctgagtga
     7801 gaccggcgac gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc
     7861 ggagcagttc ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgttgcc
     7921 ggaggccctg aaaaaggacg tgaaggggct gatcaccaag ctggagaacg tggccgacct
     7981 gagcgtcctg cagacggaca gcggtatcga tgtggcgggg ataatcaacg gcggcggctg
     8041 cctgtacgtc atcggctcga tggacgatga ggcggtgatc cgggtgcaga agatgctgtt
     8101 tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc cggaggtggc cgcacgcgag
     8161 catcatgctc gatgagatta aatatctgct gtcgaagtac gtgctgaacg cgctggggac
     8221 gctgcgcagc cgggactgca acctgctgct cgcgcaccag tcgctgggcg acttcggtca
     8281 gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg gtactggata acacgccgat
     8341 ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag tgggcggcgg gccagaccgg
     8401 ggagatccgg gtggacgtgg agcgccgccg ggccagccgg gaggcgggga acgtggagca
     8461 catcagcggc gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct
     8521 gcaacacctg cctgacgggt tcgcggtgat gaccgggctg ggcgtggcgc ggctggcgtt
     8581 cagcagcccg ctgcgggttg agcgccggga aatttcgctg aaaaccttcc cggtgctggc
     8641 gaaaacggac ccgctggcgg agtacctgcc tccggccgac ggcggcggac caaaagcgga
     8701 tgatgatttt gcggggcttt actgatgaca ctggagcagt acaacgtcag ccacgcgaag
     8761 cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg ggaagagctg
     8821 tgttccacgg cggagctggt tgcgcgacgg ctggggattg cggcggtaca gccggtttac
     8881 cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc gggcgaaata cccggttgac
     8941 ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg tggcgttctc gttcgacgag
     9001 gacgagccgc tgacggacgt tattccgttt cagccgtcaa gggtgtcggc ggcgcagctg
     9061 ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg agggccgggg ggccaccggc
     9121 tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag tgccggacgc gctggcggag
     9181 ctggacggca gaacggtggc atttgaggta gagcgcaccg tcaagagccg caggcggtat
     9241 caggaagtgg tgtccgggta tctgttcaac cgccgggcga acggcatcga tgaaatctgg
     9301 tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc gggcgattct ggcggtggat
     9361 gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg tggagctgga ccgggagagg
     9421 ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta
     9481 tttattgagg acgtggaaga caaagacggc gagcgctgca ttatgccaac cagcgagcat
     9541 ctggaagaca tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac
     9601 cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc
     9661 gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc aatgggaacg ggacaaaccc
     9721 acaggaatga agctgtgtga ttacctctat cgaaaggtgg agcggttcgc catcgagaac
     9781 ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgag
     9841 gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg
     9901 cgggaatata acagccgcta ttttaacgtg ccggtgcctg aactgccgga ggacaaaccc
     9961 gcccgccggt ggtggccgtt cggcaaaaaa taacgccagc accggcatgg ccggtgctgt
    10021 tattcttccc cgagcttatc ccgcatcacc tgccagagtg
//
