LOCUS       NZ_CP031446            10879 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain MDR-UNC plasmid pAB120, complete
            sequence.
ACCESSION   NZ_CP031446
VERSION     NZ_CP031446.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09769497
            Assembly: GCF_004136355.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Matthews,L., Goodrich,J.S., Weber,D.J., Bergman,N.H. and Miller,M.B.
  TITLE     A fatal case of necrotizing fasciitis due to multidrug resistant
            Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Matthews,L., Goodrich,J.S., Weber,D.J., Bergman,N.H. and Miller,M.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2018) NBFAC/Genomics, National Biodefense Analysis
            and Countermeasures Center (NBACC), 8300 Research Plaza, Frederick,
            MD 21702, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-JAN-2015
            Assembly Method                   :: PBcR v. JAN-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 380.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:20:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,876
            Genes (coding)                    :: 3,768
            CDSs (with protein)               :: 3,768
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 22 of 108
            Pseudo Genes (incomplete)         :: 84 of 108
            Pseudo Genes (internal stop)      :: 12 of 108
            Pseudo Genes (multiple problems)  :: 10 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031446.1.
            Bacteria and source DNA available from Organism available from
            Melissa B. Miller, Department of Pathology and Laboratory Medicine,
            University of North Carolina, Chapel Hill, NC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="MDR-UNC"
                     /isolation_source="right leg muscle, right leg fascia,
                     central venous catheter, dialysis catheter, surface of the
                     left leg ulcer, and left lower leg"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pAB120"
                     /geo_loc_name="USA: North Carolina, Chapel Hill"
                     /lat_lon="35.9132 N 79.0558 W"
                     /collection_date="2012-08-22"
                     /collected_by="UNC"
     gene            363..581
                     /locus_tag="DYB08_RS20450"
                     /old_locus_tag="DYB08_20050"
     CDS             363..581
                     /locus_tag="DYB08_RS20450"
                     /old_locus_tag="DYB08_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062952.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLKMMTFKALFHKVFGCFYRF"
                     /name="hypothetical protein"
     gene            815..1006
                     /locus_tag="DYB08_RS20045"
                     /old_locus_tag="DYB08_20055"
     CDS             815..1006
                     /locus_tag="DYB08_RS20045"
                     /old_locus_tag="DYB08_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     gene            complement(1050..1877)
                     /locus_tag="DYB08_RS20050"
                     /old_locus_tag="DYB08_20060"
     CDS             complement(1050..1877)
                     /locus_tag="DYB08_RS20050"
                     /old_locus_tag="DYB08_20060"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            2186..2473
                     /locus_tag="DYB08_RS20055"
                     /old_locus_tag="DYB08_20065"
     CDS             2186..2473
                     /locus_tag="DYB08_RS20055"
                     /old_locus_tag="DYB08_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2460..2774
                     /locus_tag="DYB08_RS20060"
                     /old_locus_tag="DYB08_20070"
     CDS             2460..2774
                     /locus_tag="DYB08_RS20060"
                     /old_locus_tag="DYB08_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            2902..5313
                     /gene="znuD2"
                     /locus_tag="DYB08_RS20065"
                     /old_locus_tag="DYB08_20075"
     CDS             2902..5313
                     /gene="znuD2"
                     /locus_tag="DYB08_RS20065"
                     /old_locus_tag="DYB08_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5618..6079
                     /locus_tag="DYB08_RS20070"
                     /old_locus_tag="DYB08_20080"
     CDS             5618..6079
                     /locus_tag="DYB08_RS20070"
                     /old_locus_tag="DYB08_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6405..6614)
                     /locus_tag="DYB08_RS20075"
                     /old_locus_tag="DYB08_20085"
     CDS             complement(6405..6614)
                     /locus_tag="DYB08_RS20075"
                     /old_locus_tag="DYB08_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(6607..6909)
                     /locus_tag="DYB08_RS20080"
                     /old_locus_tag="DYB08_20090"
     CDS             complement(6607..6909)
                     /locus_tag="DYB08_RS20080"
                     /old_locus_tag="DYB08_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6902..7258)
                     /locus_tag="DYB08_RS20085"
                     /old_locus_tag="DYB08_20095"
     CDS             complement(6902..7258)
                     /locus_tag="DYB08_RS20085"
                     /old_locus_tag="DYB08_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269902.1"
                     /translation="MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKGGKKRFYKEMLDIADQQ
                     YELHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7867..8694
                     /locus_tag="DYB08_RS20090"
                     /old_locus_tag="DYB08_20100"
     CDS             7867..8694
                     /locus_tag="DYB08_RS20090"
                     /old_locus_tag="DYB08_20100"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(8877..9020)
                     /locus_tag="DYB08_RS20195"
     CDS             complement(8877..9020)
                     /locus_tag="DYB08_RS20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(9040..9615)
                     /locus_tag="DYB08_RS20095"
                     /old_locus_tag="DYB08_20105"
     CDS             complement(9040..9615)
                     /locus_tag="DYB08_RS20095"
                     /old_locus_tag="DYB08_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(9608..10558)
                     /gene="repM"
                     /locus_tag="DYB08_RS20100"
                     /old_locus_tag="DYB08_20110"
     CDS             complement(9608..10558)
                     /gene="repM"
                     /locus_tag="DYB08_RS20100"
                     /old_locus_tag="DYB08_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     ARG             complement(1054..1877)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7868..8691
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9609..10558)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaaaaaa agcaaaagaa acttggaatt cgcttcgctc ataaaacttt tttactcgct
       61 acgctcgatc caggagcaaa tttctgcacg aaacttacgc tttcttaggc taaagaggac
      121 aaaaaaatgt ttttcagagg gtctaggcgc aaattttgat agttcgctcg tagacactcg
      181 ctctatgcat ctatctaaag tcaaaatacc tctttatttc gctctcattt gcgttttttg
      241 agctgttttg tgtttttcac tatgagatct atgttttcta ggtaaaggct cttaaatcgc
      301 atttgaacgc gttctatgaa gcttttcata attaaagttt tgggttgaaa tttttaacct
      361 ggatgaatgg acaaaaaatt gattttttac gtttttctaa acaaggaaat aaatactttt
      421 ttccatgcaa aaccaagcac cggccctacg tcactttagc caattttcca atccaagatc
      481 cggcacatgc cgggattttt ttgtcacaca ccacgcatct aaagatgatg accttcaaag
      541 ctttatttca taaggttttt ggttgttttt ataggttctg aattgtgtac aaaataattg
      601 atctttttaa tactaatttt ttgatttata tagtttttag tatgtacata gtgttgtaca
      661 taaattaatg gttttttagg cttaaatata tgaaatttat aaaaaagagt ttgtacacat
      721 ggattataca agaatgtaca aagtttaacg tgaaattagg tataacatac tgaaaaatat
      781 tgaaattaat atgtacaagt tgaggataaa cattatgtca aaagaattag tcaacttagc
      841 aaatatcgtt ccttttccta aatcatgtgg attctgtgaa tcaaaggaaa atgtacaact
      901 ttttgcaggg ttaatgcttt gtagtagctg tcaggaaaac atccgaacca ctaatcctgg
      961 aatgtttaca gctaataagc aaattgaaaa aaaagcccag gattaatctg ggcttcgcat
     1021 aaggtgtatt atgttaattt tagaaattat taaatgattc caagattttc tagcgactta
     1081 taagtaattt cattacgaat agaaccagac attccttctt tcatttctaa gttgagcgaa
     1141 aaggggattt tttttccatt agcttgctcc acccaaccag tcaaccaacc tacctgtgga
     1201 gtaacatcca ttccccatcc actttttgca taaatcttac taccatttac ttctttaatt
     1261 agaagcattt ttttaacttc ttcttgagtt tctaatttaa aaggtaatcg gttatgtgca
     1321 aggtcatcgg caaaattaac ttcttgtact ggtgtaattt taagggggcc aactaaccaa
     1381 aaattatcga cctgtgttcc aatatttgta tttccaaaat taacccgctt tacttctttc
     1441 tgcattagct ctaggccagt ccgtcttgca agctcttgat atactggaac tgctgacaat
     1501 gccattgcct cacctaaagt catatctttc tcccacatag gataagttct ttttttacca
     1561 tcccatttga aaatctcatt tgttgttgct ttatgatttt ctagcccgat taaagcattt
     1621 agcatcttaa atgttgatgc agggacatat tctttatttg ctcgtgcaag agcattacca
     1681 taggtgctaa gatttttacc ctctttaata ataattacac cctgtgtttg agcttcatca
     1741 aaatagcttt taatagcttt ttcatgttgc tgagaagaaa tatgaaaatt atcttcagat
     1801 ttagttttaa tagatgaaca tgcactgaga gaaactagaa tagaaatgct gaatatagga
     1861 agtataaatt ttttcattac aaattcatgt taggggaaat tttggggctt agagtataat
     1921 atttttatta aaaaagttaa aactctttta atttaacata atggcgatta tacgaagtac
     1981 acttgtatat taatataaat atcaactact taataaaaat agaaaaatcc caaaatgccc
     2041 aaaaagccga ctttgaaaca agtcggcttt tttatgagca gttttgatac ttcttgccaa
     2101 taattttgac taaaaattac acgaatctgg ttgagtgata caattttgtg atacattatt
     2161 gtatatacaa atttgaatgt tttttatgga acagtatttt gaatgggatg aggctaagaa
     2221 tcgaaagaat cagaaaaagc atgatatctc tttcgaaact gcaagccttg tttttgaaga
     2281 tccattacgg atctcaatcc aagacagaca taccaatggt gaagaacgtt ggcaaaccat
     2341 tggacgagtt aaaggcgtac taatgttatt ggtggctcac accatttttg atgaagatga
     2401 ctgtgaaatc atccgaatca ttagtgcaag gcaagtcact aaagcggagc gaaacaaata
     2461 tgagcatggt tagatactca cacaaagagc tgaatgagaa atttggtgag aagcaagatg
     2521 ctgaaattca acgcttgctt gctaaaggca cggttcctga tgatcagcta gatctatcag
     2581 atattcctga aattacagac tggagcaatg ctgtacgcca aaatcaattt taccgcccag
     2641 taaagcaaca aacttcaatt cggttagatg ctgatgtact tgcttggttc aaagcacaag
     2701 gcaaaggtta tcaaacacgt atgaatgaaa ttttacgaga tgctatgcta aaagaattaa
     2761 aaaatcatca ataagaatgg gagcttaggc tcccattttt gacttcattt ttagggggga
     2821 ttaccattca aaaatttaaa tagtagattt tataaatttt taagtgttat attataatat
     2881 aacattaaat ttggttgttt tatgctaaat aaaagtaaac tatttttagc tttaattact
     2941 ttaggggcct cgaaaatttt attggcagct gaagggcctg ttactacctt aaatacaata
     3001 gtattaactg cacaatctga tgagttaggg tctgagctgt taggcaaaag tctaaatgtt
     3061 agtaatcagt ttattgatac tagtaaactt aaacagcgtt cgacaactct aggggatgcc
     3121 ttgggtacgg aattaggaat tcattctaat caatatggag gtggagcatc tactcctatt
     3181 attcgtgggc aggaaggaaa acgtattaaa gttttacaga ataatgctga cgtgttggat
     3241 atgtctaata tgtccccaga tcacgctgtc acagttgaac caagtttagc taagtctatt
     3301 gaaattatac gtggtgcaag tacccttttg tatagttcaa atagtgcggc aggggtagtc
     3361 aatgtcattg actataaaat tccaacccaa atgcctcaag acggattaga gggtaatact
     3421 acattacgtt ttaatactgg cagtaatgag aagttgacca cagcaggtgt aacagttggc
     3481 ttaagcccac atgtagcttt acgggcagaa gggttatatc gtaatgctgg taactataaa
     3541 actcctcatt accagtctag ctcttataat tctttagaag atcttgaaaa ccaaaacagt
     3601 atttataaaa atttaaaata tttaccagaa agctgggcag agtcgagagt aggtacgctt
     3661 ggattatcct ggatagacga taatacttat ttaggagttt cttatacaca tcgtcatgat
     3721 gaatatggtt taccagccca tagccattta tatgaaggtt gtggtgcctc tgcaatcggt
     3781 atcgattcac gaattagtgg attaaaaaat tatcttttat attatcctca attgatggat
     3841 gagcaagata ttaattacat aaatccacgt cctgattgtc atcagcataa tcatattcat
     3901 gaaactaatt tctctcataa tgctccttat attgatttaa atactcgacg ttatgatgtg
     3961 cgtggagaat tcactcaacc atttaccggt attgacaaaa ttcgtacctc attgagttat
     4021 atagattatt ttcacaatga actcgaggga gataagatta ctaacttttt taaaaataca
     4081 ggaaaagtag gccgcataga gttatcacat cagccattag gggaattaac cggtatcttg
     4141 gggctacaat atttggaaca ggataatagt gctttatcac cagtccattc tcaagaaggc
     4201 cataccacct atctagataa tcagcagcta ttaaatcgaa atgtcactaa aaactttagt
     4261 gtatttggcc tagaaaaata taactggaac gactttactt tcgaactagg tgctcgtata
     4321 gaaaagcaga aagttagtat ggattatgat atagaaaaaa ttaaagattc aatgaaacct
     4381 tggcctaata aatataacag tccttatgta gaaaaaaata ataaaatcag ggctcaaaat
     4441 ttaaaatcta ttttagaagc tgttcaacct aacaaagaaa cagctttttc ttatgcaggt
     4501 acagtccact ggagatttgc tccaaattat atcttatcat taacaggtac acatcaagaa
     4561 agattaccta atgcacaaga aatgtatact catggtatgc atctcgctac taattctttt
     4621 gaaataggta accgctttct aagaaaagag aaatccaata atttagaaat cagtctggcc
     4681 tataaggatg atttacttga ttatcaaatc agtacttact attatgattt tgataactat
     4741 atatatttac agacactcaa tgaagtttta ggaactacaa aagttagaga tcagcataca
     4801 ctacgtatta atcattatag ccagtctgct gctaattttt atggattaga gggcaacata
     4861 gggtatcaat ttaattctgt ttatcacggc tctctttttg gagattatgt gaaaggccgt
     4921 ttaacaaatt tacctgatgc tgtaattgca tacgatatct ggaatagaga accgacttta
     4981 gctccccaaa aggatcgtta cactcctaga ttacctcctg ctcgtttggg tacacgacta
     5041 aaagctgatt ttgatgaatc gcttaaaggt gaaatagaat attatcgagt ctttaaacaa
     5101 gataatattt ctaaatttga gcaggttaca tcaggttata atatgcttaa tatgacttta
     5161 gcatataaaa ataagctatc tcacacagaa tatgatttat tctttaaagc gaataattta
     5221 cttgatcaga aagtctatgc ccatgaaaca tttttgccat atattcccca aattggaaga
     5281 aattttagtt taggcttaaa tcttaatttc tagataagat actgtaatct tctaagaaaa
     5341 tttcttagaa gatttttctt ataaaatgat tagattaata attaaggtaa gcgtccgctg
     5401 aatcccatac ctatttttta attcgattta ggtttccagc agtgtggcag taactcttca
     5461 atctgggtca ctttatgtgt cggcagcctt ttcagcactg aatagccacc gattttgtag
     5521 acacctttta gctagataaa aagcaatttt tttgatcttt aaagaatgag gatataagct
     5581 gtttaaaact taattctaat tggttaatag gtgaaaaatg aaattagcag aagctctctt
     5641 acttcgaagt gatcaacaaa aaaaaattat ttcactaaaa caacgtatca atgcaaatgt
     5701 attggtacaa gatggtgatc aaccatctga agatccaaat gaactactaa aacaagtatt
     5761 ttctttaatt caagaatttc aaaaattaag ttatgcaatt catgaaacta atgctttaac
     5821 aaaattaaat gatggccgtt ctctacttgc tttattgact ttgcgtgatg aatttgtaga
     5881 acagcacaaa accctaactg cagcaataag taatacatct cgtgaatctg atcgttatag
     5941 tacgcgggaa ataaagtggc ataaagttat tccagtttca tcattacaaa aacaagctga
     6001 tgatattagt cttaaattac gtgatttaaa cgtgttaatt cagtcgacta attggaaaat
     6061 tgatttgatt gaagcctaat agtcctttag atataatcaa gttgaacata ctgggcgagt
     6121 acaagatccc ttacttccac aacataggat tgtaaataaa gtgtagtggc ttgcaaagca
     6181 agtggataag cttaaaattt taagtacaaa tcaatccatc acttggcata actcaaatct
     6241 ttgtgcaaac aaagttacac gttacttata actaccatgt actgacagta tgttcacctc
     6301 aattactaaa aataaagaaa tgaacaagca tcagctcaac tacatttcta tttttaattc
     6361 agttcggcat ttaacataaa atctcttatg cgaatctgga aggattaagc tatttcgtaa
     6421 tattttatcc aataagcatc gtcaaaaata tcaatgtaat agcttgacct cttacaattt
     6481 gaaaaatagt cacagcataa atctctaata atttcaattt cttctaggct atcggcagca
     6541 ttgactaaaa atatgaactt gtccaagtta acaagtaatt tagtactatt tttcaaaggt
     6601 ttattattag acattaaatc ctatgtgttt tcctgttgga agctctacat caatacgaag
     6661 ttttccgccc attgcttcaa catacttttt catggttgaa attttaagat catgaccacg
     6721 attttcgatt gctgacaatg acggctgttt gattccaagg gttgcagcca gttctttttg
     6781 agaaatctct aactcttcac gaataaggtg aagctgagtt tccaacagta attcatcagc
     6841 catttgttga atacgagctt gactctctgg agagcgacta gccaataatt cttgtagggt
     6901 cttagccatt agattgatct cctaaagttg agagatgaag ttcgtattgc tgatcagcaa
     6961 tatctaacat ctctttataa aaacgctttt taccgccttt atcaccgata cacagcacaa
     7021 tagcttgtcg taaaggatca aatgcgaaaa aggctctcag tggtttacct cgatgctgaa
     7081 cacgtagctc tttcatattg gtaaatttgg agtcatagac ggtatctact aaaggacgac
     7141 ctaaacttgg tccctgttgc tgtagaacga ccaacgctgc gagtactttc tcttgtgtag
     7201 attcatcttg ctgtgcaagc cattcgttaa acagatccgt tgtaatgact gtccacatat
     7261 tacaaccata atatagatta cagtctatat tttatgctta attgaattat tattcaaatg
     7321 acaataaaaa agcccgacat cctgtctggc tttttcgttt ttgcatctca agtgtaactt
     7381 ctactttgct tcacgatcct agtgcgctca cttatgatag ttgttttgtg tgctggagca
     7441 tcctgctctc tatgtgtcca tgatagaaaa ttaagctgag tctaactatt ggttaatacc
     7501 ctcaatcggt gtgcgcttag gcgtacataa ttggcgtata tttatcccaa gatttatccg
     7561 caaattttgt ggattatttc aaaatgctga ctgttgagaa atcaaagaaa actgaatttt
     7621 tgaatatttt ttaatctatt ttattggcta aaagtgacaa agaagcttaa aaaataccca
     7681 acttgtttcc gagtcggcat tctggtattt tttggcgtta tgtagaatac ataaatattt
     7741 gatttaatta aaataattta aattgatttc gcataacgcc cattatgtta aattaaaaga
     7801 gttttaactt ttttaataaa aatattatac tctaagcccc aaaatttccc ctaacatgaa
     7861 tttgtaatga aaaaatttat acttcctata ttcagcattt ctattctagt ttctctcagt
     7921 gcatgttcat ctattaaaac taaatctgaa gataattttc atatttcttc tcagcaacat
     7981 gaaaaagcta ttaaaagcta ttttgatgaa gctcaaacac agggtgtaat tattattaaa
     8041 gagggtaaaa atcttagcac ctatggtaat gctcttgcac gagcaaataa agaatatgtc
     8101 cctgcatcaa catttaagat gctaaatgct ttaatcgggc tagaaaatca taaagcaaca
     8161 acaaatgaga ttttcaaatg ggatggtaaa aaaagaactt atcctatgtg ggagaaagat
     8221 atgactttag gtgaggcaat ggcattgtca gcagttccag tatatcaaga gcttgcaaga
     8281 cggactggcc tagagctaat gcagaaagaa gtaaagcggg ttaattttgg aaatacaaat
     8341 attggaacac aggtcgataa tttttggtta gttggccccc ttaaaattac accagtacaa
     8401 gaagttaatt ttgccgatga ccttgcacat aaccgattac cttttaaatt agaaactcaa
     8461 gaagaagtta aaaaaatgct tctaattaaa gaagtaaatg gtagtaagat ttatgcaaaa
     8521 agtggatggg gaatggatgt tactccacag gtaggttggt tgactggttg ggtggagcaa
     8581 gctaatggaa aaaaaatccc cttttcgctc aacttagaaa tgaaagaagg aatgtctggt
     8641 tctattcgta atgaaattac ttataagtcg ctagaaaatc ttggaatcat ttaataattt
     8701 ctaaaattaa cataatacac cttatacgaa atgctgtata attatcctta taattcaacg
     8761 ctgtagtttc tagtttatag cccgagcatc cctgttcggg ctttttttat gacttttcac
     8821 gttccacgct attatttcat caatgttcca cacttttgat tagctcaatt gaataatcag
     8881 gatgcctggt ctttttcttt ttggcattca tccagctctt tcttcagcgc ctttttatcc
     8941 tggtacgatg caatcagcat gatcgggatc agcagcggag ctacaataat tcccagggca
     9001 atgtctttca gtttcggcat gatctttcac ccttattgct cagtcattat caaagacggc
     9061 attcagcaca cggccaaata aaccacgttt tttgtgttgt ggcactgcca cactttgcaa
     9121 cgtggcatgc tgtagcgttt gggtgtggta tgccgtagtt tcttgctgtg gcactgctac
     9181 attttctgca atatttaatt cattattctt tttgttatca gtgtcttgtt gtgttgtcgc
     9241 atgctgtggc atcttggtgt cgcatgccgt agcatcttgg tgtggtgctg ctgtggcagg
     9301 tgcaacactt ttcagctcaa tgagtgtttg catacggtca atttgttgca ttaaccgttc
     9361 ttcacgttct tggtattttt tgacttcatt ttctaaatgg gcaataagtt tttttgtaga
     9421 ttcatcacct tcttgcttag atgattcaat tttttgagat gtttgctcag gttctccaaa
     9481 gcatcgtatg acctctgcta gatcgaattg tccatcactg gagcgtgata aacgtccatt
     9541 acgtacagct ctgtaaatgg ttgatctatc cacatgaaat tttttagata cttcacttag
     9601 tgataactta gccatttacg gtgtcctttt tatttgatgg catgtatccc acttttttta
     9661 ggtatgggta tagctctttg aattgagcgg gatcttgtag catctcagca atacgtacag
     9721 caaactgttg gtagctttct gttccttgtg aatagcgacc catttcaggg agttctgaaa
     9781 gtttatttgc aaacatatgc cgttgagcat cggtcatctt tgaaaaaagc tccaaagtgt
     9841 tcggatctct ttctattgat gggctatcct tcttcttctg tttaaaggta aaagaaaatc
     9901 ctgaaataga acgtcctctt ttatgctgtt catattttac agttatatcc gtatgctcat
     9961 ttatttgttt aattgaaagc tctaaaacac gctcttttaa atgagccatc cgtaaatatt
    10021 cattatcaag aacgcctaac ttctttctaa attcttgtag ttctatggtt ggggttttac
    10081 cagtgcttcg ccactgaatt agaagctcat ataagcgaat ggcataagcg ctactaagac
    10141 tactaacttg ctgtaattca tatttagtaa attgttcttc cagtcgggtt atgaagggaa
    10201 caacagcagg tgcaaatatt aagtctacag ttgcttcatt gtcgttataa gcaatttgag
    10261 atacccaacg actcataaca tttcggatat tcccattagc ttttttctct tgatagctaa
    10321 attgacgcgc gaataaatcc ttacaagcat cttttaaggc ttgataagct gtattacggt
    10381 gtacaccaaa ttgattgata taactatctg catgaacttc taatggatca tttgcattaa
    10441 tccctttccc actttccctt gcttcaacaa tagccaataa aattaaacgt tgttctacta
    10501 agtctaagtt atagcttgcg ttgattaagg cattgtcctt tacaactaaa tctctcatag
    10561 ccactatttt ttattgtgta taagtacacg ttaatattat ttgtgtacta atgcaaggta
    10621 aacgtggaca tattgcaagg taaacgtgga catattgcaa ggtaaacgtg gacatattgc
    10681 aaggtaaacg tggacatata agagctaaaa ccctttattc ataaggcttt caagccattt
    10741 aaaagtattt aaaaacttta aaataattaa aagcccaggg aatgaaaaat tgcccttggt
    10801 tttcgcttcg ctcaaactct attgaacttc gctttcgctc agttcgttgg ggcaattttt
    10861 tggttaatac tggcgtgac
//
