LOCUS       NZ_CP030774            10866 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Shigella flexneri 1c strain Y394 plasmid pNV-Y394, complete
            sequence.
ACCESSION   NZ_CP030774
VERSION     NZ_CP030774.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06706581
            Assembly: GCF_002442995.2
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Parajuli,P., Rajput,M. and Verma,N.K.
  TITLE     Plasmids of Shigella flexneri 1c strain Y394 are enhance bacterial
            fitness in host environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Parajuli,P., Rajput,M. and Verma,N.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2018) Research School of Biology, Australian
            National University, 46 Sullivans Creek Road, Action, ACT 2601,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2018
            Assembly Method :: HGAP v. HGAP3.3 SMRT Pipeline v. 2.3.0, Pilon
            Assembly Name                     :: ANU Verma Lab
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002442995.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:04:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,785
            Genes (coding)                    :: 4,012
            CDSs (with protein)               :: 4,012
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 773
            CDSs (without protein)            :: 773
            Pseudo Genes (ambiguous residues) :: 0 of 773
            Pseudo Genes (frameshifted)       :: 277 of 773
            Pseudo Genes (incomplete)         :: 441 of 773
            Pseudo Genes (internal stop)      :: 181 of 773
            Pseudo Genes (multiple problems)  :: 120 of 773
            Pseudo Genes (short protein)      :: 1 of 773
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030774.1.
            Bacteria and source DNA available from Verma Lab from ANU.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /strain="Y394"
                     /serotype="1c"
                     /isolation_source="Human stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /plasmid="pNV-Y394"
                     /geo_loc_name="Bangladesh: Matlab"
                     /lat_lon="23.3480 N 90.7082 E"
                     /collection_date="1988"
                     /collected_by="Nils I. A. Carlin"
                     /note="multidrug resistance plasmid"
     gene            complement(285..569)
                     /locus_tag="B7485_RS24860"
                     /old_locus_tag="B7485_26895"
     CDS             complement(285..569)
                     /locus_tag="B7485_RS24860"
                     /old_locus_tag="B7485_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            768..>1409
                     /locus_tag="B7485_RS25915"
                     /pseudo=""
     CDS             768..>1409
                     /locus_tag="B7485_RS25915"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1516..1929
                     /locus_tag="B7485_RS25920"
     CDS             1516..1929
                     /locus_tag="B7485_RS25920"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            2028..2897
                     /locus_tag="B7485_RS25925"
     CDS             2028..2897
                     /locus_tag="B7485_RS25925"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            2958..3170
                     /locus_tag="B7485_RS24870"
                     /old_locus_tag="B7485_26905"
     CDS             2958..3170
                     /locus_tag="B7485_RS24870"
                     /old_locus_tag="B7485_26905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            3172..3378
                     /gene="urfF"
                     /locus_tag="B7485_RS24875"
                     /old_locus_tag="B7485_26910"
     CDS             3172..3378
                     /gene="urfF"
                     /locus_tag="B7485_RS24875"
                     /old_locus_tag="B7485_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            3408..4247
                     /locus_tag="B7485_RS24880"
                     /old_locus_tag="B7485_26915"
     CDS             3408..4247
                     /locus_tag="B7485_RS24880"
                     /old_locus_tag="B7485_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            4234..5085
                     /gene="repC"
                     /locus_tag="B7485_RS24885"
                     /old_locus_tag="B7485_26920"
     CDS             4234..5085
                     /gene="repC"
                     /locus_tag="B7485_RS24885"
                     /old_locus_tag="B7485_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            5393..6208
                     /gene="sul2"
                     /locus_tag="B7485_RS24895"
                     /old_locus_tag="B7485_26930"
     ARG             5394..6205
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6654..7072
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             6270..7069
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7072..7907
                     /locus_tag="B7485_RS24905"
                     /old_locus_tag="B7485_26940"
                     /pseudo=""
     CDS             7072..7907
                     /locus_tag="B7485_RS24905"
                     /old_locus_tag="B7485_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            8213..8455
                     /locus_tag="B7485_RS24915"
                     /old_locus_tag="B7485_26950"
     CDS             8213..8455
                     /locus_tag="B7485_RS24915"
                     /old_locus_tag="B7485_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(8487..9137)
                     /gene="tetR(A)"
                     /locus_tag="B7485_RS24920"
                     /old_locus_tag="B7485_26955"
     CDS             complement(8487..9137)
                     /gene="tetR(A)"
                     /locus_tag="B7485_RS24920"
                     /old_locus_tag="B7485_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            9243..10442
                     /gene="tet(A)"
                     /locus_tag="B7485_RS24925"
                     /old_locus_tag="B7485_26960"
     ARG             9244..10439
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<10409..10597)
                     /locus_tag="B7485_RS24930"
                     /old_locus_tag="B7485_26965"
                     /pseudo=""
     CDS             complement(<10409..10597)
                     /locus_tag="B7485_RS24930"
                     /old_locus_tag="B7485_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     ARG             7114..7904
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.62"
                     /coverage="94.96"
     ARG             7165..7907
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.19"
                     /coverage="88.85"
     Typing          524..679
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          3426..4247
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          769..2894
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct gcccttgccc
       61 aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct acacaccgcc
      121 ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac accaaacccc
      181 gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta cccgaagggt
      241 gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc cggctcatcc
      301 ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag gtacgcatcc
      361 attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc caaaatcatg
      421 gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg ttcccttgcc
      481 cgcacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt ggccagccga
      541 tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg
      601 taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg ggagggcgca
      661 cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc
      721 gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc
      781 accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc aaggccgact
      841 acatccagcg cgaaggcaag tatgcccgcg acatggatga agtcttgcac gccgaatccg
      901 ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgtatg
      961 aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc
     1021 tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgagcgcc
     1081 tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga
     1141 tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca
     1201 acggcaagac cccggagaag ggcggggcac agaagaccga agcgctcaag cccaaggcat
     1261 ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg
     1321 gccacgacgc ccgcattgac cacagaacac ttgaggcgca gggcatcgag cgcctgcccg
     1381 gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatccgc accgaccggg
     1441 cagacgtggc cctgaacatc gacaccgcca acgcccagat catcgactta caggaatacc
     1501 gggaggcaat agaccatgaa cgcaatcgac agagtgaaga aatccagagg catcaacgag
     1561 ttagcggagc agatcgaacc gctggcccag agcatggcga cactggccga cgaagcccgg
     1621 caggtcatga gccagaccca gcaggccagc gaggcgcagg cggcggagtg gctgaaagcc
     1681 cagcgccaga caggggcggc atgggtggag ctggccaaag agttgcggga ggtagccgcc
     1741 gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca ctggaagcta
     1801 tggctaaccg tgatgctggc ttccatgatg cctacggtgg tgctgctgat cgcatcgttg
     1861 ctcttgctcg acctgacgcc actgacaacc gaggacggct cgatctggct gcgcttggtg
     1921 gcccgatgaa gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct
     1981 gtgagcgctt cgatatcggc gtcagggacg ccaccaccgg ccagatgatg aaccgggaat
     2041 ggtcagccgc cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca
     2101 atgacgtgta tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc
     2161 tcagcgagtt tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg
     2221 aaaccagccc gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac
     2281 ttcgggggca gattgcccgg acgctggcca gcgagtacga cgccgacccg gccagcgccg
     2341 acagccgcca ctatggccgc ttggcgggct tcaccaaccg caaggacaag cacaccaccc
     2401 gcgccggtta tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc gccaccgctg
     2461 gcccggcgct ggtgcagcag gctggccagc agatcgagca ggcccagcgg cagcaggaga
     2521 aggcccgcag gctggccagc ctcgaactgc ccgagcggca gcttagccgc caccggcgca
     2581 cggcgctgga cgagtaccgc agcgagatgg ccgggctggt caagcgcttc ggtgatgacc
     2641 tcagcaagtg cgactttatc gccgcgcaga agctggccag ccggggccgc agtgccgagg
     2701 aaatcggcaa ggccatggcc gaggccagcc cagcgctggc agagcgcaag cccggccacg
     2761 aagcggatta catcgagcgc accgtcagca aggtcatggg tctgcccagc gtccagcttg
     2821 cgcgggccga gctggcacgg gcaccggcac cccgccagcg aggcatggac aggggcgggc
     2881 cagatttcag catgtagtgc ttgcgttggt actcacgcct gttatactat gagtactcac
     2941 gcacagaagg gggttttatg gaatacgaaa aaagcgcttc agggtcggtc tacctgatca
     3001 aaagtgacaa gggctattgg ttgcccggtg gctttggtta tacgtcaaac aaggccgagg
     3061 ctggccgctt ttcagtcgct gatatggcca gccttaacct tgacggctgc accttgtcct
     3121 tgttccgcga agacaagcct ttcggccccg gcaagtttct cggtgactga tatgaaagac
     3181 caaaaggaca agcagaccgg cgacctgctg gccagccctg acgctgtacg ccaagcgcga
     3241 tatgccgagc gcatgaaggc caaagggatg cgtcagcgca agttctggct gaccgacgac
     3301 gaatacgagg cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg cgggggtagt
     3361 gaccccgcca gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg gctacccata
     3421 agcctatcaa tattctggag gcgttcgcag cagcgccgcc accgctggac tacgttttgc
     3481 ccaacatggt ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc ggtaaatcca
     3541 tgctggccct gcaactggcc gcacagattg caggcgggcc ggatctgctg gaggtgggcg
     3601 aactgcccac cggcccggtg atctacctgc ccgccgaaga cccgcccacc gccattcatc
     3661 accgcctgca cgcccttggg gcgcacctca gcgccgagga acggcaagcc gtggctgacg
     3721 gcctgctgat ccagccgctg atcggcagcc tgcccaacat catggccccg gagtggttcg
     3781 acggcctcaa gcgcgccgcc gagggccgcc gcctgatggt gctggacacg ctgcgccggt
     3841 tccacatcga ggaagaaaac gccagcggcc ccatggccca ggtcatcggt cgcatggagg
     3901 ccatcgccgc cgataccggg tgctctatcg tgttcctgca ccatgccagc aagggcgcgg
     3961 ccatgatggg cgcaggcgac cagcagcagg ccagccgggg cagctcggta ctggtcgata
     4021 acatccgctg gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag gaatggggtg
     4081 tggacgacga ccagcgccgg ttcttcgtcc gcttcggtgt gagcaaggcc aactatggcg
     4141 caccgttcgc tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag cccgccgtgc
     4201 tggagaggca gcgcaagagc aagggggtgc cccgtggtga agcctaagaa caagcacagc
     4261 ctcagccacg tccggcacga cccggcgcac tgtctggccc ccggcctgtt ccgtgccctc
     4321 aagcggggcg agcgcaagcg cagcaagctg gacgtgacgt atgactacgg cgacggcaag
     4381 cggatcgagt tcagcggccc ggagccgctg ggcgctgatg atctgcgcat cctgcaaggg
     4441 ctggtggcca tggctgggcc taatggccta gtgcttggcc cggaacccaa gaccgaaggc
     4501 ggacggcagc tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc tgatgccatg
     4561 gtggtcaaag gtagctatcg ggcgctggca aaggaaatcg gggcagaggt cgatagtggt
     4621 ggggcgctca agcacataca ggactgcatc gagcgccttt ggaaggtatc catcatcgcc
     4681 cagaatggcc gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag cgacgaggcg
     4741 gacgggcgcc tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat gggtggcggc
     4801 cagcatgtgc gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac cgcccgcctg
     4861 ctgcaccagc ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc ttccatagat
     4921 accttgtgcg gctatgtctg gccgtcagag gccagtggtt cgaccatgcg caagcgccgc
     4981 cagcgggtgc gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt aaccgagttc
     5041 gcggcgggca agtacgacat cacccggccc aaggcggcag gctgaccccc cccactctat
     5101 tgtaaacaag acatttttat cttttatatt caatggctta ttttcctgct aattggtaat
     5161 accatgaaaa ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga
     5221 gcgctgccgc acagctccat aggccgcttt cctggctttg cttccagatg tatgctcttc
     5281 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     5341 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     5401 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     5461 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     5521 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     5581 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     5641 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     5701 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     5761 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc
     5821 tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg
     5881 tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc
     5941 tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga
     6001 tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg
     6061 tccgggggat gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc
     6121 tgacttcatc cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc
     6181 gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg
     6241 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
     6301 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
     6361 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
     6421 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg
     6481 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
     6541 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
     6601 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
     6661 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
     6721 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
     6781 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
     6841 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
     6901 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
     6961 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
     7021 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
     7081 ccgcctgttt ttcctgctca ttggcacgtt tcgaacctgt tctcattgcg gacacctttt
     7141 ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc aagggattga
     7201 aacctataga agacattgct gatgaactgc gcggggccga ctatctggta tggcgcaatg
     7261 ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc gaatatgccg
     7321 gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg accgaaattg
     7381 cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct tctgcccttc
     7441 tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag
     7501 gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg agcaatgcct
     7561 cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg
     7621 gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc gccgccaata
     7681 tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc attgcacaga
     7741 tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt
     7801 acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag caacgcgatc
     7861 tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata tcaagcgact
     7921 tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc aaagccttag
     7981 cgtaggattc cgccccttcc cgcaaacgac ccctggaagc cccgtagcga cgcggagagg
     8041 ggcgagacaa gccaagggcg caggctcgat gcgcagcacg acatagccgg ttctcgcaag
     8101 gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac gcgagccatt
     8161 cgcgagagcc ttgagtccac gctagatcta tctcatctgc gcaaggcaga acgtgaagac
     8221 ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc
     8281 cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag aggccgagcg
     8341 cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg gctgctacgg
     8401 gcgtctgacg cggtggaaag ggggagggga tgttgtctac atggctctgc tgtagtgagt
     8461 gggttgcgct ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc
     8521 ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac
     8581 gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc
     8641 ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca
     8701 agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc
     8761 gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg
     8821 cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg
     8881 aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa
     8941 tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc
     9001 ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc
     9061 gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc
     9121 ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact
     9181 tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag
     9241 acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg
     9301 gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg
     9361 acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg
     9421 cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc
     9481 tggccggcgc tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct
     9541 atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata
     9601 ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt
     9661 tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc
     9721 acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc
     9781 ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc
     9841 tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct
     9901 tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc
     9961 gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt
    10021 cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac
    10081 tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg
    10141 gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc
    10201 tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac
    10261 tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg
    10321 cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc
    10381 tctgcctgcc ggcgctgcgt cgcgggcttt ggagaaattc ttcaaattcc cgttgcacat
    10441 agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt
    10501 caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc
    10561 gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt
    10621 gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc
    10681 gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac gaacccctgc
    10741 aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac tgtcacgccc
    10801 ccaaacctgc aaacccagca ggggcggggg ctggcggggt gttggaaaaa tccatccatg
    10861 attatc
//
