LOCUS       NZ_CP030769            27128 bp    DNA     linear   CON 17-JUN-2024
DEFINITION  Escherichia coli strain 2017C-4173W12 plasmid pMCR-1_2017C-4173W12.
ACCESSION   NZ_CP030769
VERSION     NZ_CP030769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09534372
            Assembly: GCF_003308975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27128)
  AUTHORS   Lindsey,R.L., Batra,D., Smith,P.A., Patel,P.N., Tagg,K.A.,
            Garcia-Toledo,L., Loparev,V.N., Juieng,P., Sheth,M., Joung,Y.J. and
            Rowe,L.A.
  TITLE     High Quality PacBio Genome Sequences of an Escherichia coli O157:H7,
            Diffusely Adherent E. coli and a Salmonella enterica, all carrying
            plasmids with a mcr-1 Resistance Gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27128)
  AUTHORS   Lindsey,R.L., Batra,D., Smith,P.A., Patel,P.N., Tagg,K.A.,
            Garcia-Toledo,L., Loparev,V.N., Juieng,P., Sheth,M., Joung,Y.J. and
            Rowe,L.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2018) EDLB, CDC, 1600 Clifton Road, Atlanta, GA
            30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003308975.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 04:08:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,443
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 5,117
            CDSs (with protein)               :: 5,117
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 62 of 205
            Pseudo Genes (incomplete)         :: 152 of 205
            Pseudo Genes (internal stop)      :: 39 of 205
            Pseudo Genes (multiple problems)  :: 43 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..27128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017C-4173W12"
                     /serotype="O153:H2"
                     /db_xref="taxon:562"
                     /plasmid="pMCR-1_2017C-4173W12"
                     /geo_loc_name="USA"
     gene            complement(194..460)
                     /locus_tag="DSB71_RS27785"
     CDS             complement(194..460)
                     /locus_tag="DSB71_RS27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228299791.1"
                     /translation="MLIQDLKKAFIYIGHTKMSAFIKLASVLALFISPVVVAIAGEQSH
                     CTKINEYPFIVTQCDDGTVTVVNVINNRVAVCRKGESCKEINL"
                     /name="hypothetical protein"
     gene            complement(681..1334)
                     /locus_tag="DSB71_RS27795"
     CDS             complement(681..1334)
                     /locus_tag="DSB71_RS27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053899054.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGVS
                     GSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKFLVFGGLFGLSARLFTFLSDTGSDFAE
                     PVTTTTATGKIQSILGGMAEMVSILGSVVPFLVIIIAMIWFLFFSSKREKEELTRVERE
                     AAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALADESNNVS"
                     /name="hypothetical protein"
     gene            complement(1345..1515)
                     /locus_tag="DSB71_RS29435"
     CDS             complement(1345..1515)
                     /locus_tag="DSB71_RS29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000550721.1"
                     /translation="MHLSLLIPLTGAAMNALCIFIIRLLDKDFIEVYKKTLKKITALHI
                     LLMVLSVTGIV"
                     /name="hypothetical protein"
     gene            complement(1519..1962)
                     /locus_tag="DSB71_RS27800"
     CDS             complement(1519..1962)
                     /locus_tag="DSB71_RS27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="WP_000964333.1"
                     /translation="MLWFTLFLLCIALEIITGTGWLLLISLGALSSAIVGFFLPISQEA
                     NICFFASISILASIIKFLYDKKHKKLDTLLVNTGHSRFKGKEFTLSDDIVNGKGQLLIG
                     DTFWPVETLHNENYPAGTRVIVTDMQGITLKVMTIEDKSENKE"
                     /name="NfeD family protein"
     gene            complement(2348..3301)
                     /locus_tag="DSB71_RS27810"
     CDS             complement(2348..3301)
                     /locus_tag="DSB71_RS27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="WP_072097371.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLSP
                     GLHFLIPFMDRIGQRINMMETVLDVPKQEVISKDNANVTIDAVCFIQVIDAAKAAYEVD
                     NLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIEIRD
                     VKPPEELTKAMNAQMKAERTKRAQILEAEGIRQSQILKAEGEKQSQILKAEGERQSAFL
                     QSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKLVMMPL
                     DSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
                     /name="SPFH domain-containing protein"
     gene            complement(3328..3504)
                     /locus_tag="DSB71_RS29440"
     CDS             complement(3328..3504)
                     /locus_tag="DSB71_RS29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000753050.1"
                     /translation="MADFGSTKQTVTFEEWHELLMDYAELRGGNAADAEAWRGDYEAGK
                     TPVEAYCDEWGED"
                     /name="hypothetical protein"
     gene            complement(3497..3712)
                     /locus_tag="DSB71_RS27815"
     CDS             complement(3497..3712)
                     /locus_tag="DSB71_RS27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="WP_001127357.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAGK
                     CFSVDVHISDFICKEIPPQNAGNTYG"
                     /name="DUF1187 family protein"
     gene            complement(3705..4157)
                     /locus_tag="DSB71_RS27820"
     CDS             complement(3705..4157)
                     /locus_tag="DSB71_RS27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="WP_223666486.1"
                     /translation="MARKTKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVSR
                     NFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKKEI
                     AYASYDNIPSVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
                     /name="CaiF/GrlA family transcriptional regulator"
     gene            5297..6325
                     /gene="repA"
                     /locus_tag="DSB71_RS27825"
     CDS             5297..6325
                     /gene="repA"
                     /locus_tag="DSB71_RS27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_001175213.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGTT
                     KRRRGEHSTKPKCENPAWFRPDNYKALGGQLGHAYNRLVKKDPVTGQNTLRMHMSLHPF
                     YVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDSKGD
                     VIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKMLGVDL
                     VKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRRKKAAE
                     RKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLELALTASP
                     PH"
                     /name="repA"
     gene            7026..7216
                     /locus_tag="DSB71_RS29955"
                     /pseudo=""
     CDS             7026..7216
                     /locus_tag="DSB71_RS29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            7866..>8018
                     /locus_tag="DSB71_RS29960"
                     /pseudo=""
     CDS             7866..>8018
                     /locus_tag="DSB71_RS29960"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
                     /name="DUF5431 family protein"
     gene            7963..8085
                     /locus_tag="DSB71_RS27845"
     CDS             7963..8085
                     /locus_tag="DSB71_RS27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="WP_223170659.1"
                     /translation="MVCCTLLIFTLLTRNRLCEIRLKDGYREVTASMAYESGGK"
                     /name="type I toxin-antitoxin system Hok family toxin"
     gene            8235..8831
                     /locus_tag="DSB71_RS27850"
     CDS             8235..8831
                     /locus_tag="DSB71_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_000125165.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSAV
                     NENKKVQKKDAVAEKTRKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWTDE
                     KKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRYDLY
                     GKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
                     /name="ProQ/FINO family protein"
     gene            complement(8849..9133)
                     /locus_tag="DSB71_RS29535"
     CDS             complement(8849..9133)
                     /locus_tag="DSB71_RS29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001162371.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRLG
                     DVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
                     /name="hypothetical protein"
     gene            complement(9207..9872)
                     /locus_tag="DSB71_RS27860"
     CDS             complement(9207..9872)
                     /locus_tag="DSB71_RS27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000532148.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNNDI
                     LSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVADI
                     LLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDLDKL
                     LRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELGINP"
                     /name="division plane positioning ATPase MipZ"
     gene            10460..10789
                     /locus_tag="DSB71_RS27870"
     CDS             10460..10789
                     /locus_tag="DSB71_RS27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000855119.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTVQ
                     EIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIEEFETAYKGSPEEKHE
                     FTSYY"
                     /name="hypothetical protein"
     gene            11193..11708
                     /locus_tag="DSB71_RS27875"
     CDS             11193..11708
                     /locus_tag="DSB71_RS27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025393.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSADEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            11812..12354
                     /locus_tag="DSB71_RS27880"
     CDS             11812..12354
                     /locus_tag="DSB71_RS27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083904.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCPSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWSSPYHVCTLSRKRNPVDIAANIERKILLNASQEVLQA
                     IEYEKRQAAKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGERYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            12351..12605
                     /locus_tag="DSB71_RS27885"
     CDS             12351..12605
                     /locus_tag="DSB71_RS27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230709.1"
                     /translation="MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLS
                     ELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYKESDA"
                     /name="hypothetical protein"
     gene            12602..12844
                     /locus_tag="DSB71_RS27890"
     CDS             12602..12844
                     /locus_tag="DSB71_RS27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000151456.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQ
                     LNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP"
                     /name="hypothetical protein"
     gene            13415..13708
                     /locus_tag="DSB71_RS27900"
     CDS             13415..13708
                     /locus_tag="DSB71_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000673428.1"
                     /translation="MKFTSTTNHVFTFERVTLCTIVLIHKDTGQQYVVIFTDNNKIRDY
                     KTGIVSQFGELKQSDIDLILFYRDEYEKYFDSLNNGEEYLSFKEYIGCIRGK"
                     /name="hypothetical protein"
     gene            complement(14125..14568)
                     /locus_tag="DSB71_RS29965"
     CDS             complement(14125..14568)
                     /locus_tag="DSB71_RS29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192625.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAYS
                     FISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKRIV
                     INRLLSGSILTGKDEDYIYSQLKKAEQSNEREKRLQAIKEYTS"
                     /name="hypothetical protein"
     gene            14865..15047
                     /locus_tag="DSB71_RS27910"
     CDS             14865..15047
                     /locus_tag="DSB71_RS27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000833470.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKD
                     LPLGTVRSIKKMAGI"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            15072..15509
                     /locus_tag="DSB71_RS27915"
     CDS             15072..15509
                     /locus_tag="DSB71_RS27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000466318.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAMA
                     REAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQRI
                     NISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(15640..15966)
                     /locus_tag="DSB71_RS27920"
     CDS             complement(15640..15966)
                     /locus_tag="DSB71_RS27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032345720.1"
                     /translation="MTTFTDDDKGLIKEIRERIGSLDVRDNIERRAYEIALASLEAEAP
                     NLPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
                     /name="hypothetical protein"
     gene            complement(15968..16291)
                     /locus_tag="DSB71_RS27925"
     CDS             complement(15968..16291)
                     /locus_tag="DSB71_RS27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236922528.1"
                     /translation="MEAGVVAWRYRYVKKDVTDSQGKTWVGDWKYVPTKEDCNDRPNYE
                     IQELYNLPPAIAAPTRGLVNAVRFYDQVKHTNPPVETGAWKDAIDWVLKEAYQAVNIDA
                     KGE"
                     /name="hypothetical protein"
     gene            complement(<16268..16408)
                     /locus_tag="DSB71_RS29970"
                     /pseudo=""
     CDS             complement(<16268..16408)
                     /locus_tag="DSB71_RS29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(16428..17135)
                     /locus_tag="DSB71_RS27930"
     CDS             complement(16428..17135)
                     /locus_tag="DSB71_RS27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000108794.1"
                     /translation="MSTVNQPELKQPEKAVSSEDIDNFIVDVFKETGHKISKDDPVISL
                     IFLNQKIQEKFSNELQANFTALSEGFRQVVSSVENDYIQRFKNIVETCGDLDNEIKEKV
                     EEGKNDLKETSVEVKEKLTDDIIELISGIKRNQEKNNKLYEEKLKSLSKAVKPFSTRTA
                     IAICALCTLCLSAAFSGATWYVAQHEKEASLRFYARAFLDMKKITEESMRKLPKSDQQN
                     IKLKLDEIDSRKP"
                     /name="hypothetical protein"
     gene            complement(17137..17889)
                     /locus_tag="DSB71_RS27935"
     CDS             complement(17137..17889)
                     /locus_tag="DSB71_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="WP_000797966.1"
                     /translation="MKNSINFILQGKGGVGKSFATAILAQYFIDENHMDNIVVGDTDPV
                     NTTTVKVKRLNADLIQITENSKVIQSKFDPMFESMLTNSQNTFVIDNGASTFLPLIQYF
                     NDNCVMDMFEDVEQDVYIHTVIVGGQALADTLQGFEELKELVKGSKVKLIVWINEFQGI
                     PALENIPLIETKFIEKNKDVIAGVVVIQDRKSDAFASDIKELTEKSLTLKEALESDHFG
                     LMAKSRLKRVFNDIYKQLDSIYDDEHGE"
                     /name="conjugal transfer protein TraL"
     gene            complement(17907..18308)
                     /locus_tag="DSB71_RS27940"
     CDS             complement(17907..18308)
                     /locus_tag="DSB71_RS27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021575837.1"
                     /translation="MKKIESLLSPICEAINETKSKSKQAVFNMYLQNILEAKKYYTWEQ
                     IADYINNNTKTELAQKTYINMTERAKKRQKCTEPKEQAKTIDVIDQNEAKGDEPRGGVK
                     GFFSNISENKKEIEHDPSATNEKFINKYL"
                     /name="hypothetical protein"
     gene            18676..19017
                     /gene="mobC"
                     /locus_tag="DSB71_RS27945"
     CDS             18676..19017
                     /gene="mobC"
                     /locus_tag="DSB71_RS27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000730722.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIGK
                     INTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLIGI
                     RDRLQEIAK"
                     /name="mobC"
     gene            19014..23327
                     /locus_tag="DSB71_RS27950"
     CDS             19014..23327
                     /locus_tag="DSB71_RS27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="WP_034169409.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTD
                     KIIDSIENKSQERYLHITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHL
                     PKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQEFIN
                     NKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSVFKDI
                     VAEYGELRIRNAGKTNEYLAVKLPGDKKYINLKSPLFRQNYIETRTLTLEKPTHKEIEK
                     RLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQ
                     TSGRAGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDL
                     AEQLSQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKNEIATMAEI
                     RRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLTKHINLAWKD
                     AKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSEKTLRNTIRFERGKLYN
                     DLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQYHADWHKDKDE
                     MKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSKLKDLVMDKQDSR
                     IVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCA
                     EVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQESESRIEKGDALKEQA
                     TEQEQATEQAQATEQAQATEQAQATEQAQATEQEQATEQEQVTEQEQATEQEQATEQAQ
                     ATEQAQATEQAQVAAQATSSYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIW
                     EVLGHVPGDSDDIFDVASFDNENDAKEFCKIVNELGIDRTQALIQEQLTTQHDQATAQV
                     HNKQEIYCINFSRFHDLNEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVL
                     MSKTVSTDELSSYPEGALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESL
                     HDASEYKNKMLQEHGLNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESP
                     EEILASISANEHMISGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYHLAVAGKHGL
                     ERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQ
                     KDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDA
                     VMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHG
                     FTREMAEDALGKQFVQAREEHLELQQQRDNSQDMH"
                     /name="LPD7 domain-containing protein"
     gene            23548..25173
                     /locus_tag="DSB71_RS27955"
     CDS             23548..25173
                     /locus_tag="DSB71_RS27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            25245..26018
                     /locus_tag="DSB71_RS27960"
     CDS             25245..26018
                     /locus_tag="DSB71_RS27960"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_052234578.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQFKEKKSTPNRNNSYKNGQ"
                     /name="PAP2 family protein"
     gene            complement(25977..26417)
                     /locus_tag="DSB71_RS29310"
     CDS             complement(25977..26417)
                     /locus_tag="DSB71_RS29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000666084.1"
                     /translation="MKEYEKAALIYSLKESNTLVLRIILISILVVGVISGAVYLYNLAI
                     SLFDSNPTSVQFTVCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAIII
                     ISFITMALTASLVLKDSSNIWLNITFIALLLSIFVAVISVWG"
                     /name="hypothetical protein"
     gene            complement(26763..26969)
                     /gene="hha"
                     /locus_tag="DSB71_RS27970"
     CDS             complement(26763..26969)
                     /gene="hha"
                     /locus_tag="DSB71_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850854.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNSLPPSELESFNAAADH
                     RLAELTMNKLYDKIPPSVWKYVY"
                     /name="hha"
     ARG             23549..25170
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5310..6325
                     /name="IncI2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19015..23324
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaattttcct tgtttcgatg agattgattt ttacttgtgg tatatcacgg aagacaccac
       61 agccggtaat tgcttttgaa acgacacgtt ctgtattcgc tatgcttttc tttactgttc
      121 gaggggggac ataacgcgca cggcgaagtt ttctcagatt agcttttgct tgagtcatgt
      181 aatatgtcca gagttataag tttatttctt tgcatgattc ccctttacgg cacacagcta
      241 cacggttatt tatcacattc accactgtca cagttccgtc gtcgcattga gtgacgatga
      301 aagggtattc gttgattttt gtacagtgag attgttctcc tgctattgca accacaaccg
      361 ggctaataaa taacgccaga acagatgcca gcttaataaa agcactcatt tttgtatgcc
      421 ctatatagat aaatgctttc ttcaaatcct gaataagcat aaaccaggaa catggcggct
      481 aaaggcaaag aaaagatgaa atgaataacg gcattaagaa ctgatccgat taatgtataa
      541 aagacaaacg gtgccgagag tgtgaaagca ccaagtagaa aagaaaaaat attgaacgcc
      601 atttttaata aaaacatttt cattaggtga tgtttttttc tgtacatttc atcgtatcct
      661 tcagggggct gtcaccttct tcatgagacg ttgttgcttt catcagcaag tgcctcaata
      721 agtttattaa tacacgcccg ttctgccgga acctcccggc ctgtaatcgg gtcaaaaacg
      781 acatgatcgc tttcaacgta acgtaaacgg tgatagacat ctatacggag ttttgtttta
      841 gcagcttcac gttcaactcg tgttaactct tctttttctc gtttgctgct gaaaaacaga
      901 aaccagatca ttgctatgat aattactaaa aaagggacaa cgctgcctaa aatgcttacc
      961 atttctgcca tgccacccag aatagattga atctttcctg tggctgtggt ggttgttact
     1021 ggttcagcaa aatcagaacc agtgtctgat aaaaatgtaa ataaacgggc agataaacca
     1081 aatagcccgc caaaaacgag aaattttatt atcgaaccgt atacatttaa tagactgtat
     1141 tcttcctctt taggcgattc aaagtttttt gccctttgtg tctgctcttg tttagaacca
     1201 gaaacacctc cgatgaataa gccaatacca ccagtaccta aaccaatacc cccaccagta
     1261 ccattccatt tacctgttgt tgtttcttta gctacaatag ctgcaagaga aattccttca
     1321 tttgcgtttc gcatagaatt gtcctcatac tattcctgtg acacttaaca ccatcaataa
     1381 aatatgtaat gcagtaattt tttttagcgt ttttttatag acttcaataa aatccttatc
     1441 cagaagtctg ataataaaaa tacatagtgc attcatcgct gctcctgtaa gcggaattaa
     1501 caacgaaaga tgcatgattt attccttgtt ttcactcttg tcttcgatgg tcattacttt
     1561 aagagttatt ccttgcatgt ctgtaacaat aactcttgtt cccgcagggt agttttcatt
     1621 gtgtaacgtc tcaacaggcc agaatgtatc accaataagt aattgccctt ttccgttcac
     1681 aatatcgtca ctgagagtaa attcttttcc tttaaaacgt gaatgccctg tgttcaccag
     1741 gagagtatct aatttcttat gttttttgtc atagaggaac ttaattatcg acgccagtat
     1801 gctgattgat gcaaagaagc atatattggc ttcttgtgat atcggaagaa aaaagcccac
     1861 aatggcgctt gataatgcac caagactgat taagagtaac catcctgttc cggttataat
     1921 ttcaagtgca atacacagaa gaaacagtgt aaaccataac atggctaatc tccatgataa
     1981 aagggagaaa ttgaataaag aagatgaagg tagttataaa cgccaaatgc ataaattagc
     2041 agaaagaagc cgaagaatat gccattaaaa ctccatatta acaggggcag cagagccatc
     2101 gctatcatca cccctccgaa gttcagccat aatggaaata ttgatgcgat gtggtttagt
     2161 attttttctc ctttgttctc atcttttgta ataaaaagaa gaaagctcca tgcccccata
     2221 gggattatat gtaaaataag aaggatgaac ccataagttt tcatttaaat gatttgctca
     2281 ttcgtgaata tgaatatgaa tatgaggacg gtgattaaaa caatattgtc accgtccgct
     2341 atctgtttta agacaaatat tgttgagcct gtttcatatc aactttggga gtatcctgtg
     2401 atacgttttt tagcagttca ctgatacctg caaccgaact caccaggcta cttgaatcaa
     2461 gcggcatcat caccagttta ctgtttgatg ctgtgccaat tgcctggagt gcttcagtgt
     2521 atttttgcgc aataaagtaa ttaacagact gaacgtcacc ttcagcgatt gcatccgaca
     2581 ccagttttgt tgcgcgagct tccgcttctg cctgacgttc gcgtgcttca gactgaagaa
     2641 acgctgattg ccgttcccct tctgctttca gtatttgaga ctgtttttcc ccttccgcct
     2701 tcagaatttg tgactggcgt ataccttccg cttccagaat ctgtgcacgc ttagtacgtt
     2761 ccgctttcat ttgcgcgttc atcgccttag tcagttcttc tggtggttta acatcccgaa
     2821 tttcaatacg tgtgacctta attccccacg gatcagttgc ataatcgaca accgttaaca
     2881 gcttagaatt gatgctgtca cgttgagaaa gcatatcgtc gagattcatt ccaccaacga
     2941 cagttcggat attggtcatg acaagatttg aaatagctga tgcaagatta tcgacttcat
     3001 aagctgcctt cgctgcatca atgacctgga taaaacaaac agcatcgata gttacgtttg
     3061 cattgtcttt cgaaataact tcctgtttag gaacatccag gacggtttcc atcatgttga
     3121 tccgttgacc tatgcggtcc ataaacggga tcaggaagtg aagaccgggg gaaagcgtat
     3181 gggtatattt accaaagcgt tccactgtcc aggcattacc ttgcggcacg attttgacgg
     3241 cagatttaac gaatatcagc gcgactaaaa ttaataagag taacggaata gatgtaacta
     3301 ttggcgtaat tatagcgtca atcatgatta atcctctccc cattcatcac aatatgcttc
     3361 aaccggagtt tttcctgctt cataatcacc acgccatgct tccgcatcgg cggcatttcc
     3421 accgcgtaac tctgcgtaat ccattaatag ctcgtgccat tcttcaaatg ttacggtttg
     3481 tttagtggaa ccaaaatcag ccatatgtat ttcctgcatt ttgtggaggt atctctttgc
     3541 aaatgaaatc tgaaatatga acatcaacag aaaaacactt tcctgcttct ttttcttttg
     3601 agaagttttt tctgcattct tcagcggtca tagggaagtc gctgtaatga gtccatttca
     3661 cggggccacc acctggtttt ttgatggttg cgtttatttg gtacttaggc attcaaaaat
     3721 acctccactc gatcacctgt ctttcttgaa cacatccagc ggcgcagttc tgtaaaacta
     3781 tcctggatct cacaacgggt acttttcatt gtgacactcg gtatattgtc gtagctggca
     3841 tatgcaatct ctttttttgg ggtgcgcaat atgcctttgt caatatcaag aatcctgaac
     3901 gcactaattc ttcttttgtg ctcgttaaat agcgttcgct tagttgctgt tatctgcttc
     3961 tgtgcttcct ggtaaatgta ggtaagcaca tcataagatt gttgctgtgt aataaggaaa
     4021 ttacggctaa catccgttgt tgtgagcgtt ttattatttc ttaatcccca ataagccacg
     4081 atgatataga gtggatatcc tttcaaatct gccacacatt caggaatgac aggattaatt
     4141 tttgtcttac gcgccatatt ttctcccggt tgtttgtctg aaatagaaac atccccggag
     4201 tacagcgtca taagctaatt atgaaaagtt gcagccactc actacaggct ctttatctga
     4261 ttgttcatca tcatgcatag ctaaaccccc ttacctggag tggagactgg caaatctggt
     4321 gtacttctgc gttgcagcct gtacgacttg ctcagggaga gagtgagtga gattaactca
     4381 acgcgagttc accaaataag ggggtttaag tatgcagaat gatatttgcg gataaagagt
     4441 cgttacctgt gtagatggcg cggcacgtca ctctgttacg tagcccgttg ctgacagatg
     4501 ccagcggtgc ttgaaatgca cgaataataa cctccttatg ccagggcttt aggcaatgac
     4561 cgctgtccgg ggttgttaaa gagcatgaga tgacaagcgt tctaacgggc gtcaaagagt
     4621 gctcattttc caggcaatga gcacaaaaaa cgcgatgaac gagcttttta caatcatctg
     4681 gatagtattg tgtgtgcgac atttaaaacc tgtcaatgcc acgagagcga tctaatgatc
     4741 tggataacac ctaaaaaata gaataatatt ctatttgtta aatgatgtac gatctaaaaa
     4801 tctagataat actgtttgtg tggtttattc gatcgtgtga atgggtgtat gcagtcaaaa
     4861 aaactttttt gaatatacaa aattctgaat ttctgatttt gtaaggtttt gtatggggaa
     4921 cgtcagtaaa gaaggggctt cagtagctgt tctgaaaacg attttgaagc agtcacagat
     4981 gtgtgctgaa tcaaaaagga aacgagccag gcttgaacat cgttgatcga ttgcgcccat
     5041 gctgctaaat ttgcaggcaa gttttccgcg atttgtaaat gcagaaaacg aggtgctgaa
     5101 attttgttcg agaacgtaaa tttcagataa agaaaacccc cgtaatctcg tcttttccag
     5161 ggagcgcaga ttagcggggg ttgacctcaa ggcagagcct tgagctgata acattttatt
     5221 tcaggtaatc gcttactacc tgaagtggag tttaattcca tgagaaggaa atatcaactc
     5281 ttacagtgca agctaagtgc agcttgctgt gattagcaaa cgtcactggt ctgaactatc
     5341 accggaagag caggtccgtt tctggcagga ctacgaagcg ggtattgaaa gctcattcct
     5401 ggtcccccag gaaaacaaag gcggaacaac aaaacgtcgc cggggtgaac attccactaa
     5461 gccaaagtgt gagaatccag cctggttccg tcccgacaac tataaggcgc tgggcggaca
     5521 gttgggccac gcttacaatc gtctcgttaa aaaagacccg gttactggtc agaacacact
     5581 cagaatgcac atgtcgttgc atccatttta tgtactgaaa cgtcagagtg tcggtcgcaa
     5641 atataaattc cgaccggaga aacagcggct gcttgacgca atatgggttg tacttgtgag
     5701 tttttgtgat cgtggattgc acactgtcgg catgtctgtc tcccgcctgg cagaagaaat
     5761 aagcccaaaa gacagtaagg gagacgtgat tcctgaaacc gcagtcacag tatcgcgcct
     5821 ttcccgtctg ctggcagagc aggtttcctt tggcacgttg ggaacgtcag aaaagacgat
     5881 atgggaccgt gaatcccgcc agcgactgcc gaaatacgtc tggatcaccg aaactggctg
     5941 gaaaatgctg ggagttgatc tggtgaaact tcaggaacag caacgtaaac gccttgcaga
     6001 aagtgaaatc cgtctgcaat tgattaaaga aggcgttatc cgcgagggcg aagaaatctc
     6061 tgttcactcg gctcgtaaac gctggtatgc gcagcgttct ctcgatgcca tcaagtcccg
     6121 acgtaagaaa gcagcggagc gcaaacgtgc taatcgcctg gcaaaactgc cgtatgatga
     6181 acagcgaaac gaaattgcgc ggtttattct gaagcgtatg ccgccggacg aagcgtactg
     6241 gtgcactaaa gaaagattag agcaactggt agccagagat ctacgtcagc ttgaactggc
     6301 cctgacagct tcgccacccc actagtttag ctgcctgata caatcggcac tctcttcagc
     6361 cccttcgggg cttttcgtgt tgcctgcatt tcatgaaatt cctcatattt gacgaccgtt
     6421 tttcattttg tcccgctctg acagtttttt gatggccgca atagggtatc ggagtaaaaa
     6481 cgccttcggt tatccacaaa aacccgcaaa ggaatagctt cagaaagcag caaacataga
     6541 acatcaaacc cgcaatatat tcttaacccc tgttctttaa tcccctgcgt tgcttcgccg
     6601 cagggaaaat atttatcttt gaaacaactg tggataatta caaaaatgcc ttcgcttgca
     6661 gcggctaacg ccgcgccgct cagaatataa aagtacctcc caccgcttcg cggcgggcat
     6721 atggacttct gcctaatgaa aaacaaagcc cctccaaccc tcttacaaaa aaacgctgca
     6781 aatactcagc cacagcgccc aacccatcac tgaaaagcgc cgccgccccc gcccgaaggg
     6841 cgggaacaac atcgctttca ataatggatg ttgtaactaa gaaattacat ggctgtcagt
     6901 cttctggctg gaagtaccaa gtacacgctc gtaagcggtc ctggcggccc gctaacgcgg
     6961 agatacgccc cgactgcggg taaacccttg tcgggaccac tccgaccgcg cacaaaagct
     7021 atttcatggc tgaaagcggg tatggcttag caggatgggg atgggtaagg tgaaacctat
     7081 caatcagtac cggcttacgc cgggcttcgg cggttttact ccagtatcat atgtaacaac
     7141 ggagtgccgc cttccatgcc gctggcgcgg catcaaaaaa gaaagccccc caatgacggg
     7201 ggaaatagcg ttttgatcaa aataataatg ctagtgtgct tcttgcgtgc cattccaggc
     7261 acaaactaaa tttaacgttt cgttaactac aaagaaatcc gatgagttaa tcacttgcga
     7321 aataaaaatc atatagtaaa ttactatatg attttgcgtt gtttcggaga attaaaaaat
     7381 gcctttgatt gaatacatca acaagttcta tcgggggaat caggcatcgt tcgccaggct
     7441 gaccggagtt caacctgcgc aggtaacaca gtggatcaac aaaggattca tcgtggtgaa
     7501 tcacacactg tatagtccac gccgaaagct gggaatttaa ttcctggtca atatttgaca
     7561 tcctccacgc cctgaaggac ggggatataa ggcgcactgt atcctgttgt ctggcagaaa
     7621 aaagaaagcc ccgtagttaa tttttcatta acccacgagg cgatcccaat gcttcaacaa
     7681 caccaggata gacttttacc gccctttacg caataccatg tattttcaag tttctggtcg
     7741 atatataatg ttgttggcgc ttgaggcttt ctgcctcatg gcgaaaaggt ggtttgtatc
     7801 ttgttgtgcg gcagaaagaa gaaagccccg tagttaattt ttcattaacc cacgaggcga
     7861 tcccaatgct tgaacaacat caggatagcc tcttaccgcg cgttacgcaa ggagaagaag
     7921 gccatgaaac taccgggaaa cgcccttatc tggtgcgtat taatcgtgtg ctgcacgttg
     7981 ttaatattca cactcctgac ccggaatcgc ctgtgcgaaa tccggctgaa ggacggatac
     8041 agggaggtta cggcaagcat ggcttacgaa tccggcggta agtagcaacc cggaggcggg
     8101 cgaaagcccg cctttttggg gatgatgtgg ttctggcatt aagcgccttt acaaaggggg
     8161 tattctgtac ggataccccc tttttccatg tattttcagg atattaactg gtgaatatgg
     8221 tataattagt caatatgaca gcaattattg aggcgagtat gcagcccaaa aaaacacccg
     8281 tgattgtggt caaaaaacgc cgcgttctgg tcatgccaga aaatcctgtg gtgaatgaaa
     8341 agccgcagga agtacaaaaa tcagcagtta atgaaaacaa gaaagtacag aagaaagatg
     8401 ctgtagcaga gaaaacacgt aaaaagcagc ctcagccctg gtatttgaaa aaacaaatca
     8461 cttttccccc aaaatatccg aaagaatatt ttgaaaagtg tttcaataaa gttcgtgctg
     8521 tttttcctga actatggaca gacgaaaaaa agaacttgcc cctgaaaagc gggattctcc
     8581 aggacgttga gaaatacctg gcggataacc cggatgtgga tctgacgatt gaagagtgga
     8641 attgtgcggt tcaggtgatg acgttccggt ggcaatatct tcagaattgt actgtaccag
     8701 gcgcaacacg ttacgacctt tacgggaaac ctgctggtac agtaaaaaaa gcacacgcaa
     8761 cttatgcgca actggttctc gatgcccgta agaaagccag cgagaagaaa caattaaaac
     8821 gtaagggata aagccccgaa gggctttatc atttcagcct ggctgactgt agaatccagt
     8881 gattttttcc gttttcgtcc aggccgtgtt cgtaagctgc aagagccgct ttcattattg
     8941 ctgtttttgt ctggcctgtt cgcagtgctt cccgttccag aatatcttcg taaacgtccc
     9001 caagccggac attggtcact ttagttgctc ccgatttttt gcgggtagct tcattgatga
     9061 atcgggctgt gtctgcattg gtttgttctg ttttttgtcc tggtattacc ggagttttca
     9121 agtcaagagc cattatatca cctttcgtgt gttacattag ctttactgtg taaagttaat
     9181 gatgtaaagc tgtcgtatta catccattag ggattgatgc ctaactcttg cgccaaaagc
     9241 tctatttgtg ctttagctgt agaaaggctg gatgcgcgac gtacatcatg aacgccagcg
     9301 ccttcattac aggccgcttc aaaaatatct aaatctgaaa tacgtgtacg aagtggctgt
     9361 atccacaccg ggttctcccg gagtaacttg tcgagatcaa tcgctgcttt tctgtggcgt
     9421 ggttttgtcg tgttaattct cgtgaaaagt acccagggtt gcaggtctgg attgatctgt
     9481 tgagctgtgc gaactttttc tgttacatga gtcagcgttt ctgcttcgaa atctgaagac
     9541 ggcttaacga gagtaagcag aatgtcagct acagttaagg cactgcgaaa ttccttgctg
     9601 tcatgccctg gacagtcaat gatcagcact tcacatattt ttttcaggcg cttaatgact
     9661 tctgatacat cgccatactc ttcataaaca ggaaccggta ataagccatt ctgctttctt
     9721 ttttcgttcc agctaagaat atcattgttt ttatcggtct tcagaatgat gaccgttcgt
     9781 gaattattta ccagataaga agccaggttt gttgctgtgg tgctttttcc tacaccgcct
     9841 ttatctgatg cgacaagtaa tgctctcccc atagaaacct cggatataca gtgtaaaggt
     9901 ttatttgtct ttacattgta ttcattatag ttacgttgta aagatgtttg tgtctttaca
     9961 atgtaattgt ttttgtgctt tacattgtgt gtatgttaca gggtaacttt tcaggatgcg
    10021 attttgcctg gtgtgttgtt gcgcccgtag ctcgctgacg ctcacacgcg cagtacagcg
    10081 ccccgccctg ctgcaatgca ccaggacggc gtaaactcgc tggcgctcgc gctaactgcg
    10141 gcattctgcg gcgcaaatca gcatgatcat ggcctgtcag tccgttgggc tttgcagggt
    10201 gaagggatgg ccctgtgcat cctggtatgt tcgtcagcgg ctgcgccgcg cttcggggcg
    10261 atgtggcccc gctgctccct ggtatttcct ttcgcccctt ctatccgctg acgcggatta
    10321 agaaatgagc aaatcatcaa gtaaattgct gttttcaggc aataaatctt gataatacat
    10381 ctaaatagat gtattattat ttcatcgaaa ggggattggc tcctttcctt cttaagtcca
    10441 aacggacagg agatttaaaa tgaaaacatt gacttttaat aacggcactg tttctgttgg
    10501 cgatgtgttt gtatcttcct ggggatatga gcaaacgaac gttaatttct accaggttat
    10561 ttctgttcat ggcaaaaaaa ccgtaactgt tcaggagatt cgcgcttcag ttcatcggac
    10621 tcattcaatg agcggatata agaccccatt acttaatgat ttctgtggtg agccattaaa
    10681 acggcgagtg cgtgattatt acagtacgcc agcaattgaa attgaggaat tcgaaacagc
    10741 atataaagga tcaccggaag aaaaacacga gttcacatca tactactaag aaaatagttg
    10801 cagggggtat cccctgcaac catgttaaaa atgttaatag tagtagtatt tacccagggg
    10861 gttttagtat gaatgatggg gtaaaactcc gtccttcagg gcgtggagaa tgccgggcta
    10921 atgcagcaat gtaatgttgc gcccgtagct cgctgacgct cacacacgca gtacagcgcc
    10981 gcgcttcggg gcgatgtggc cccgctgctc cctggtattt tctttcgccc cttctatccg
    11041 ctgacgcgga ttaagaaacg agcaaatcat caagtaaatt gctgttttca ggaaataaat
    11101 cttgataata catctaaata gatgtattat tgtttcatcg aaaggggatt ggctcctttc
    11161 cttcttaagt ccaaacggac aggagattta aaatgaatat ccaggaagca ttaaacgttt
    11221 ttggattatc cggcgaatta actgaaaaag atatcaaagc agcatacaga aaagccgctt
    11281 taaaatatca tccagatcgt aacccgttag gggctgaact gatgaaagcg gtaaatgcag
    11341 cttttgatgt cttgatggca aatattgata aaataaatca gttccagagc gctgatgaac
    11401 atgcacgata taattacggt gatgacctgg aaaaagtatt aaacgttctt tctggtttat
    11461 ctggtctggt atttgaagtg ataggtaact gggtatggat tagtggagaa actattacac
    11521 ataaggaaac tttaaaagaa atcgggtgta aatgggcggc aaagaaaaaa caatggtttt
    11581 atcgtccaga cgaacataaa agttactgga atcgtgaaga acacacgata gaagaaatcc
    11641 gcgcaaaata cggtacaacc ggacagcgca gggcgacagg gtggcaacgc gtggaaacca
    11701 gagcgtaacc agaacggggg cgagaagccc ccgatatcat accagcccgt cttttggggc
    11761 atcgaaccca aaaagacggg aacagcagcc acgtagcaaa aagggaaaca gatgaactct
    11821 ttttttgaac aatatcaccc tgtctttgaa gttgtctgcc gtatcctggg gaacggctgg
    11881 cgcgtgaaca aacttgatga ttgtccgtct cgtataaaac tgacgtcacc gcagtttaaa
    11941 aattactctg tgcatattcg gatggaaaaa gaccgatttt ctgttgtggg gagtgtggat
    12001 agtcgttcct ggagtagtcc gtatcatgtt tgtacgttat ccaggaagcg gaatcccgtt
    12061 gatatagcag ccaatatcga gcgaaaaatt ttgctaaatg cttcgcagga ggtgttacag
    12121 gcgattgagt atgagaaacg ccaggcggca aaaaaggacg aaattctgat cctgaaaggt
    12181 atgttatcgc aacttgtcca gcttgaaagc tggtatgggg cattgacagg ctttaaagct
    12241 gaaaatggat tgaacggtaa agtcaccgag cagggcgagc gttatgattt gcagattagg
    12301 ggtttgagta tagatcaact tgttaaaatt acaggatatt tgaaacagtt atgagaaaaa
    12361 acaaagggcg attgacttat tatcttgaag tgattgataa aaaataccat tttgtaaaaa
    12421 aaataagcag ttattcaaag gagttcactg acggaaaaac aaaaagaaca aagagaacgt
    12481 taagtgagct ggtttttaat gaaagtgagg tcgaggcaat agactttaca aaaaatggtt
    12541 taagacctgt tgataagaat attctcttaa ctatggtgaa agaatataag gagagtgatg
    12601 catgactatt gaaaatacac cggaaaacat aaagaaatta cgtaaaaaaa ttggccttac
    12661 ccagacagag tgcggtgaga tttttggcgt aggcttaagt acatggcaga aaaaagaagc
    12721 taaaacccac aaccagctta atttatcgaa aggtgaattt gagtacctgt tattacttgc
    12781 aggggagcac cctgaatacg tcctgcataa aaaagctgaa tcccgctcac cggaggaacc
    12841 gtgagcgaga agaaaaacac cacgacgcag aaatctaaga tatacagtgt aaaggtttat
    12901 ttgtctttac attgtaattg ttctttgtct ttacacagtg tgtgtttgta acagggcgac
    12961 tttacataat tgcgattttg cctggtgtgt tgttgcgccc gtagctcgct gacgctcaca
    13021 cgcgcagtac agcgccccgc cctgctgcaa tgcaccagga cggcgtgaac tcgctggcgc
    13081 tcgcgctaac tgcggcattc tgcggcgcaa atcagcatga tcatagcctg ttggtctgtt
    13141 ctggctttgc agggcgaagg gatggccctg tgcatcctgg tatgttcgtc agcggctgcg
    13201 ccgcgcttcg gggcgatgtg gccccgctgc tccctggtat tttctttcgc cccttctatc
    13261 cgctggcgcg gataaaaaaa tgagcaaacc atcaaataac ttgctgtttt caggcaacaa
    13321 atcttgataa tacatctaaa tagatgtatt attgtttcat cgaaagggga ttggctcctt
    13381 tccttcttaa gtccaaacgg acaggagatt taaaatgaaa ttcacttcaa ccacgaatca
    13441 tgttttcaca ttcgaacgag taacgctttg cacgattgtt cttattcata aggatacggg
    13501 acaacagtat gttgtcatat ttacggataa caataaaatt cgtgactata agaccggaat
    13561 tgtttctcag tttggtgaac ttaagcagag tgatattgat ttaattcttt tctatcggga
    13621 cgaatatgaa aaatattttg actcgttaaa taatggcgag gagtatttga gttttaaaga
    13681 atatatcgga tgtataaggg gaaaataaat tatgaaaggt gtgatcaggt tgctggaaca
    13741 acctgatcac gttatcgaca atcaaaactg aatgaggttt ttaaatgtca acgcggcaga
    13801 tcgtacagct tgctgactcc cttgtcaaag gctgggaaac cagaattccg gcaatgacgt
    13861 gcacggagtg ggatcaattc cggtggtggt taaactacct gcaagggtat gaaatgtttt
    13921 aaataaccgg gataaattca tatatgggga gagtgtcatg ctctcccttt cttattgtgt
    13981 aagcgagttg tattacatat ttagctttac gttgtaaaga ttttattcgt tgtctttaca
    14041 gtgtggtgcg ttttccggcg gttgcgctgc gcttaggggt atgtggcttc gatgatccag
    14101 ggtatttcga cgtaatgcgt tgtattacga tgtatactct ttaattgctt gcaatctctt
    14161 ttcacgttcg ttgctttgtt cagccttttt cagttgactg tatatgtagt cttcatcttt
    14221 acccgtgagt attgagccgg agagcagacg attaataact atacgtttaa tttctgggta
    14281 atctttggtt ttatcgagta tggacaatat gtggtcactg ctaagaatta tcccactcca
    14341 tgagtctaaa tagctcttaa aaatcacaat cataaacaca ccgaaaagca ttatgatgat
    14401 tgttaccaca ttaatgctaa acgttgatat aaaggaatac gcggccacac caattattgc
    14461 taaacagaga cataaataaa tgaaaccgat tttgttgcgt tgcttttttt ctgcacgatc
    14521 atgatcatct atgatttcca gtatcttttc tgggattagt tgagtcattt tctttcctta
    14581 tcagggacac ggagtgttta attatatagc tttacattgt aaagctatgt ttgtctttac
    14641 gatgtaaatg atgagatgtg cagttaaaag tgtcaatcag gtatcatata gtaaaacact
    14701 atatgatttt ctatgtatca catgcatagc gataaaactg gataaatgat ttacactgta
    14761 aagattgaac tgtctttaca gtgtgtttca taagtcatac gaatgaaata atttgccttt
    14821 gtgtgtatgt gtgtgtataa ttgttttcag gttagggagg acatatgaaa tctgctgacc
    14881 tgttgaaaga actgatagct gctggttgcg agttgaaacg gcataaggca agcagtcacc
    14941 agatatggtg gtcgccaata acaggaaaaa cttttcctgt tcctcatcct aaaaaggact
    15001 tgccgctagg tactgtcaga tcgatcaaaa aaatggcggg gatttaatcc ccgccgactt
    15061 tggaggtagc catgttcttt tctgttggtg tcgaaacacc gaaagatgat catacagcat
    15121 acggtatcac cgttccggtt tttgattgct ttgattttgg atgtgtttct gctgctgatt
    15181 ctcaggcaga aatacccgca atggcgcgtg aagcgatatt ggcaatcgtg gaagagatgg
    15241 taataagcgg tgctcattct gttgatgata ttcatgatga agggtgtttg acttattccg
    15301 ccaacccaaa ttataaccac tgtgatagct ggtttgtaat tgatgttgat ttgtcagaaa
    15361 ttgagggcaa acaacaacgt attaatattt cattgcctga tgtattgatt cgtcgtattg
    15421 atggatatgt gagagaaagt ggtggcgtat acaaagatcg tagccatttt ctggctcagg
    15481 cggcacgtca tgaacttgca tataaataaa actatccccg cgttgtgcgg ggattttttt
    15541 tgatagctaa ccgctcgccg cagtccagcg accgagcata gcgagcgagc gacgaggaag
    15601 cggaagatcc accgttgcca ggagaaaagc aggtacgcac tatgcgttct gccctagtga
    15661 tgctttcaca aaccacgcag cctgaactat tacgccatga atccagcgca aatcggcatc
    15721 acgattttct tttttcattt tttcgccact taaagccttg cttacgtgac tttttaccag
    15781 attttcgtgt aattcttttg cttcttcaat tgtgaaacca ccaggcaaat ttggcgcttc
    15841 tgcttccagt gatgccagag caatttcata agcccggcgc tcaatattgt ctcgcacgtc
    15901 caggctgccg attcgctccc tgatttcttt aatcagtccc ttgtcatcgt cggtaaatgt
    15961 tgtcatctca ctctcctttg gcgtcaatgt ttacagcctg gtaagcctct ttgagcaccc
    16021 agtcaatagc gtctttccat gcccctgttt cgacgggagg attcgtgtgc tttacctggt
    16081 cgtagaagcg tactgcgttt accagtcctc ttgttggtgc tgctatcgca ggtggtaagt
    16141 tgtagagttc ctgaatttca tagttcggcc tgtcgttgca gtcttctttt gtcggtacgt
    16201 atttccagtc accaacccac gttttaccct gagagtccgt aacgtctttt ttcacgtaac
    16261 gatacctcca tgcaacaacc ccagcttcca gcgatgccag tgcgatttca taagcccgac
    16321 gctcaatatt gtctcggacg tccaggctgc cgattcgctc tctgatttct ttaatcagtc
    16381 ccttgtcgtc gtcggtaaaa gtggtcataa gtgactcctt ttcatatcta tggttttctt
    16441 gaatcaattt cgtctagttt gagttttata ttttgctggt ctgattttgg taattttctc
    16501 atgctctctt cagttatttt tttcatatca agaaatgctc ttgcatagaa gcgcagactt
    16561 gcctcttttt catgttgtgc aacataccag gttgcgccag aaaatgccgc agacaggcat
    16621 aatgtgcaca gggcacaaat tgcaatggct gttcttgtac tgaagggttt tacagcctta
    16681 gataatgact tcaacttttc ttcatataat ttgttgtttt tttcctggtt gcgtttaatt
    16741 ccagatatga gttcaataat atcatctgtt aatttttctt ttacctcaac acttgtttct
    16801 tttagatcgt tttttccctc ttctactttt tcttttattt cattatccag atcgccgcac
    16861 gtctcaacaa tattcttgaa tctttgaata tagtcatttt ctacagatga aacgacctgt
    16921 ctgaagccct cagacagggc tgtgaagtta gcttgtaact cattgctgaa tttttcctgt
    16981 atcttttggt tcagaaaaat taatgaaata actggatcat cttttgatat tttatgccct
    17041 gtctccttga atacatcaac aataaaatta tcaatatctt cactggaaac agccttttca
    17101 ggctgtttta attctggctg attaactgta ctcatattat tcaccgtgtt catcgtcata
    17161 gatgctatct aattgtttgt aaatgtcatt gaagactcgt tttaatcgtg atttagccat
    17221 taatccaaaa tggtcggact caagagcttc tttcagtgtt agtgattttt ctgttagctc
    17281 tttgatgtca gaggcaaagg catcactttt tctgtcctga ataaccacaa cgccagcgat
    17341 tacatccttg tttttttcaa tgaatttagt ttctattaat gggatatttt caagtgctgg
    17401 aataccctga aattcattta tccagacaat aagttttacc ttacttcctt taaccagttc
    17461 tttcagttct tcaaatccct gcaaggtatc cgcaagagct tgtccaccaa cgataacagt
    17521 atggatataa acgtcttgtt ctacatcttc aaacatgtcc ataacacagt tatcattgaa
    17581 atactgaatt aacggtaaaa atgtagatgc gccattgtca ataacaaaag tattctgact
    17641 attggtcagc attgattcga acattggatc gaatttggat tgtattactt tgctgttttc
    17701 agtgatttgt atcaaatctg catttaatct ttttacttta accgtagttg tattaaccgg
    17761 gtctgtgtca ccgactacga tattatccat gtgattttca tcaataaagt attgtgccag
    17821 tattgcggtc gcgaatgatt ttccaacacc acctttaccc tgaaggatga aattgattga
    17881 gtttttcatt tataatgctc ctttacttaa agatatttat ttatgaattt ttcattcgta
    17941 gcacttggat cgtgctctat ttcttttttg ttctcactaa tgtttgaaaa aaaacctttt
    18001 accccccctc tcggttcatc gcctttagct tcattctgat caattacatc tatggttttt
    18061 gcctgttctt ttggttcagt gcatttttgt ctttttttag ccctctctgt catatttata
    18121 taagtctttt gggctagttc tgtcttggtg ttgttgttta tgtaatcagc tatttgctcc
    18181 catgtataat atttctttgc ttcaagtata ttttgcaaat acatattgaa gacagcctgc
    18241 tttgatttcg attttgtttc gtttatagcc tcgcatattg gagataataa actctcgatc
    18301 tttttcattt gttacatttc ctctctctct ctacctccta cattggcata ataccttatt
    18361 taaagcaatt tgctcgccgt ttgtgtgggt gattatgtgg gttgttttgt gggttgtcaa
    18421 tgggggtgtg gtcttttttg tgggttaaag tgtgggtgat tgtgtgggtt gtgtgttttt
    18481 ggttgtgggt tattatgtga gtcattgtgt gggctgtatg cacggcattt tttgttcttc
    18541 taaaacacag gcacgatacc gattgtacac aagaccgttc cggtcatgtt acaatcgtgt
    18601 gccaaagggg gacgcccctt tgaaaccccg aacgatgcag ccgttcttat gcggttctac
    18661 gaaccttgag gacatatgaa gaaaaaaacc aacaaaaacg ttcatgtaac attcagactt
    18721 accgaagaag aatatgctcc gttcgatagg gctataaaag agcttaatat tagtaagtct
    18781 gaatttttca ggctgcttac tattggtaaa ataaacacat atgcatctga taaacgtaac
    18841 ataccagaat acaaacgttg tctttctcag ttgagttggg caggaaacaa cataaatcaa
    18901 atagcgcacc gattaaattc agatcattta aaaggtatta tatcagaatc gctttataaa
    18961 aaggttttaa atggactaat cggtattcgt gatcgccttc aggagatagc taaatgattg
    19021 tcagatacgg tggtggtaat gatggtatcg ttgattatct gataaatggt cgcaaagcag
    19081 aacgccagta cacgcgtgat gaacttgatc atcgtgttgt tcttgatggt gatttacaga
    19141 ctacagataa gattattgat tctattgaaa acaaaagtca ggaacgttat ttgcacatta
    19201 ctctgtcttt tcatgaaagt catgtgtcga atgaagtgtt aaaggctgtt gttgatgatt
    19261 ataaaaaatt attgatgaat gcttatcatc ctgatgaata ctctttttat gcggaagctc
    19321 atttgcctaa aattcgtcat atccaggata atagcactgg tgaacttgtt gaaagaaaac
    19381 cacatattca tattgtaatt cctaaagtaa atttgattac tgaaaagttc ctgaatccag
    19441 tgggagatgt taccaaaggg catacaattg aacagcttga tgctatacag gaatttatta
    19501 ataataaata taatcttgat agccctaaag actatccgcg taaagatgcg gattacggaa
    19561 aaattataag tagagttaag ggggatcttt acaaagagca tcactctgaa ttgaaaggtg
    19621 agttgctttc gcgtatagag aacgagaaaa ttgaaaatta ctctgtattc aaagatattg
    19681 ttgcagagta tggtgaactt cgtataagaa atgctggtaa aaccaatgag tatctggctg
    19741 taaaattgcc tggagataaa aaatacataa acttaaaaag tcctcttttt cgtcagaatt
    19801 acattgagac aaggacatta acacttgaga aaccaacaca caaagagata gaaaagagac
    19861 ttaatacctg gttaaataaa acaagccaag agattaagca tatttttaac caggcggaga
    19921 aaacaaggga actttacaag acattaagcc cttcacaaca aattgatttc ctgcaagaga
    19981 ggataaaaga atatgactca agagaaaaac ttaacgaaag aaattctcag caaacgtcag
    20041 gacgagcggg aggttacaag tcgtgtccta aaaagtttgc cagaatccgt caatctgaag
    20101 caacagtcgg attgtcacgt atgccccaac gcggtatggt ttacggaatc aatggattca
    20161 caagacccga ctctgtcagt gtattgtcag atatttcgca gcgtgatctg gcagagcaat
    20221 tatcgcaaag agaacatcct ggtcaggatg tgcgacggga ttatgataga caattcacag
    20281 agtcaggaat aaaaagtctt gaacgctctt cttttttgtg tgaaacgatg tttcagactc
    20341 tgaatgaggc ggcagagaaa aacgaaattg caacgatggc agaaattcgc agaaatattg
    20401 atcctgtcag gtttctgtct tctgccgccg agcgttttaa tattattcca gcgcagcata
    20461 aaatcagaac agcaaaggac ggttcccccc ggttttctgt ggggaatcgt aatatgaacg
    20521 catctgattt tctgacaaag catattaatc ttgcctggaa agatgcgaaa tcctttctgc
    20581 ttgaagttta ttcacagcag ttagaaaaca cgccatatac gcgctatcca acctacagac
    20641 gccttacgca tcatgaagcc cgtgagcgtc ttaactcact gaatttatcg gaaaaaacat
    20701 tacgaaatac tatcaggttt gagcgtggca agctgtataa tgatctgcga gagatgagac
    20761 gcgagttgaa gttaatacca cgtgaacagc gtgatattgc cgtgggtgtg attgtttaca
    20821 aaaaactaac cacacttgag cgtctttctg aactcgacac agaggggcga cacataattc
    20881 gtcaatatca tgctgactgg cataaggata aagatgaaat gaaagccctt gaacgtctca
    20941 aaagctatct caacttcgat gaaatcaacg ccatttctgc tgacgaaccc gaactttcgc
    21001 ttcagaaagc ggtggattcc cagcgccgct tagaagaggc gaaaaaggtt aactccaaac
    21061 taaaagatct tgtgatggat aagcaggatt ccaggattgt ttatcgcgat caggaatcgg
    21121 aaaagcctgt ctttaccgac aaagggaact ttgtcgttgc gggtaaaaat ccttcaaaag
    21181 aagagatcgg aataatgctt gagtattcca gggaaaagtt tggtggtgta ctcaaactta
    21241 ccggctctga agatttcaaa aaaatgtgcg ctgaagttgc cgcagagcag gacatgaaga
    21301 ttattttacg cccggagcag tatcaacaga tgatgctgga attaaaagca gaacttcagg
    21361 gtaataagtt tgagcaggtg gaaacacagg aaaatagcca ggagtccgaa tccagaatag
    21421 agaaaggtga tgcattgaag gaacaggcca ccgaacagga acaggctaca gagcaggcac
    21481 aggctacaga gcaggcacag gctacagaac aggcacaggc tacagagcag gcacaggcta
    21541 cagagcagga acaggccacc gaacaggaac aggtcaccga acaggaacag gctaccgaac
    21601 aggaacaggc taccgaacag gcacaggcca cagagcaggc acaggccaca gagcaagcac
    21661 aggtcgcagc gcaggccaca tcttcatatg atcctggtgt tattaccagg gcaaacaccc
    21721 tggattcaca gatgcttagt aaaggcacaa atggtgaatt tggctatttg aaatctctgg
    21781 atagcgatga aaatgagatt tgggaagtgc tgggacatgt acctggagac agcgatgata
    21841 tttttgacgt tgccagtttt gataatgaaa acgacgcaaa agaattttgc aaaattgtga
    21901 atgagttagg tattgacaga acacaggctc tgattcagga gcagcttact actcaacacg
    21961 atcaggctac tgcgcaggtg cataataaac aggaaattta ttgtataaac tttagtcgct
    22021 tccatgattt gaatgaaggt atcgtctttc attctaaaga tgcggctatt cagtgttatg
    22081 aggaaagcaa atcttctgca atagaaaaat atgaaagcaa ttatttaaat ggtgatggtt
    22141 ttgatactgt tgtgcttatg tctaaaactg tctcaaccga tgaattatcc tcttatccag
    22201 agggggcatt agtaacgttt gataggccat ttgaaataat tgccaattca tatgaagaat
    22261 accgtactcc agtgtatgcg gtttcttttt cgaaggatga gtttagtgaa gatattaaga
    22321 cttttgagtc attgcacgac gcatctgaat ataaaaataa gatgcttcag gagcacgggc
    22381 ttaaccagga tgatatctta atcacacctg taacaagaga agaaatcgcc tttaaaggaa
    22441 ttaaggacgc tgttaatgac gctaatatgg cagtgatgga acaggcgggg gattcgtcca
    22501 gagaatcacc agaagagata ctcgccagta tttcagctaa cgagcatatg atctccgggc
    22561 ttgaaaactt cctggttaaa gaccgttctc agttcagttc ctgcaatggg gatatcgttg
    22621 tggaggcgga aattaccagg ggggaaggtg gtctttatca ccttgcggtc gctggtaagc
    22681 atggcttaga gcgtggtgat gcagttgcca gagtggatgt tacggagcag caatttgccg
    22741 cgatcactgg aaaaacacct tctgaagttt taacggggga ccaaacatcg gctcgtgttc
    22801 cggtaattac aggtatccat ttcagtacaa gggctattga aaatgtgaat aaactggaac
    22861 aacagaagga ttatgtctat ttctcaacac atgaaggcct gaatgctgaa ataaaagatt
    22921 tcagctctct caaggatgct attgagtggg gccgggttga atgcgagcta catgatctca
    22981 ataagcgcga tactgtcatt tatcgcgtcg agtctgaaca tatttctcag gggattgacg
    23041 ctgtaatgaa aaatgctgaa cgcgtggagc gtcatgaaat tgagaaagca cagggacggg
    23101 attgcacgcc ggaagacggc aagattcttg aggcaattga tcgctttgag gacaagttca
    23161 gaggggaagg actgaagttc gaaagggaga aggccgaatc cgatttgctt aatcacggat
    23221 tcactcgtga aatggcagaa gatgctcttg gaaaacaatt tgtccaggcc agagaagaac
    23281 atctggaatt gcagcaacag cgtgacaatt cacaggacat gcactaatta agaaaaaagc
    23341 ccggttttcg ggctttttaa gatacaaatt ataaatactc tcaagtgtat attcagtatg
    23401 ggattgcgca atgattgcct aataaaattt ctgaaatatt tctgtatcgc ataatttttt
    23461 atatcagata aattgtactg gatttcttaa aaaattgcag tatagttgcc gcaattatcc
    23521 caccgtttat tttttgagta gtttctcatg atgcagcata cttctgtgtg gtaccgacgc
    23581 tcggtcagtc cgtttgttct tgtggcgagt gttgccgttt tcttgaccgc gaccgccaat
    23641 cttacctttt ttgataaaat cagccaaacc tatcccatcg cggacaatct cggctttgtg
    23701 ctgacgatcg ctgtcgtgct ctttggcgcg atgctactga tcaccacgct gttatcatcg
    23761 tatcgctatg tgctaaagcc tgtgttgatt ttgctattaa tcatgggcgc ggtgaccagt
    23821 tattttactg acacttatgg cacggtctat gatacgacca tgctccaaaa tgccctacag
    23881 accgaccaag ccgagaccaa ggatctatta aacgcagcgt ttatcatgcg tatcattggt
    23941 ttgggtgtgc taccaagttt gcttgtggct tttgttaagg tggattatcc gacttggggc
    24001 aagggtttga tgcgccgatt gggcttgatc gtggcaagtc ttgcgctgat tttactgcct
    24061 gtggtggcgt tcagcagtca ttatgccagt ttctttcgcg tgcataagcc gctgcgtagc
    24121 tatgtcaatc cgatcatgcc aatctactcg gtgggtaagc ttgccagtat tgagtataaa
    24181 aaagccagtg cgccaaaaga taccatttat cacgccaaag acgcggtaca agcaaccaag
    24241 cctgatatgc gtaagccacg cctagtggtg ttcgtcgtcg gtgagacggc acgcgccgat
    24301 catgtcagct tcaatggcta tgagcgcgat actttcccac agcttgccaa gatcgatggc
    24361 gtgaccaatt ttagcaatgt cacatcgtgc ggcacatcga cggcgtattc tgtgccgtgt
    24421 atgttcagct atctgggcgc ggatgagtat gatgtcgata ccgccaaata ccaagaaaat
    24481 gtgctggata cgctggatcg cttgggcgta agtatcttgt ggcgtgataa taattcggac
    24541 tcaaaaggcg tgatggataa gctgccaaaa gcgcaatttg ccgattataa atccgcgacc
    24601 aacaacgcca tctgcaacac caatccttat aacgaatgcc gcgatgtcgg tatgctcgtt
    24661 ggcttagatg actttgtcgc tgccaataac ggcaaagata tgctgatcat gctgcaccaa
    24721 atgggcaatc acgggcctgc gtattttaag cgatatgatg aaaagtttgc caaattcacg
    24781 ccagtgtgtg aaggtaatga gcttgccaag tgcgaacatc agtccttgat caatgcttat
    24841 gacaatgcct tgcttgccac cgatgatttc atcgctcaaa gtatccagtg gctgcagacg
    24901 cacagcaatg cctatgatgt ctcaatgctg tatgtcagcg atcatggcga aagtctgggt
    24961 gagaacggtg tctatctaca tggtatgcca aatgcctttg caccaaaaga acagcgcagt
    25021 gtgcctgcat ttttctggac ggataagcaa actggcatca cgccaatggc aaccgatacc
    25081 gtcctgaccc atgacgcgat cacgccgaca ttattaaagc tgtttgatgt caccgcggac
    25141 aaagtcaaag accgcaccgc attcatccgc tgatttctcc ctgtattttt tccaaaccca
    25201 ccgcacactc cattcgtatt atgggcggtg gggtggggtt tgttatgccg tatttatcaa
    25261 ataaacgcct acttgctgag atgagtatcg ctcttgtcat ggcgatcgtt gccacgctga
    25321 cccttgagca cagtcagatt gatctgatgg tcgctgattg gttttatctg ggtatggggc
    25381 attggatggt tgccaagcaa gcttttttgc cagatttgct actgtattct ggactaaaaa
    25441 agctgctgat ggcgatgctg atctacttgc tggttgcgac catttgccgt gcttatcatg
    25501 agaaaaaggg caatgctatc actgccaagt ggcttgtccc agtgacaaaa tttcgcgtgc
    25561 gtgagcttgc gtatctggtg ctgactttga tcctagtgcc gacagttgtc gcgtcattga
    25621 aggcatatac tcatgtggtc tgccctgtgc atttgacgat ttttgatggt acgctgccgt
    25681 atttgccgat gcttgatagt atgcgtaaca ccattcctga taagtgcttt cctgcggcgc
    25741 atgccagtag cggatttgcg ctgtttgcct ttgcgtttgc gccaagtttg cgccgccgtc
    25801 gtggtgcgat catcatcgtg gtgatggcat tgggctgggc gatgggctgc tataagatga
    25861 ttattggcga tcattttttg agccatacgg tggtgtcgat gatgcttgcg tgggcgatgt
    25921 cggcagggct tgcgtgggtg ttttttaaga agggtgaaca attcaaggaa aaaaaatcaa
    25981 ccccaaacag aaataacagc tacaaaaatg gacagtaata atgcaataaa agtaatgttg
    26041 agccatatgt tggacgagtc ttttaagact aatgaggctg ttaatgccat tgtaatgaaa
    26101 ctaatgataa taatggctgc aaataaatat tgtacaatgt tttttattgt acttggttgc
    26161 tccccaattc tttctctgta atttgcatca acattagata tccattgaag taatagagca
    26221 ataaaacagc ctactccgca aacggtgaat tgcacagacg tcgggtttga atcaaacaac
    26281 gagatcgcta agttatataa gtataccgcg cctgaaatca ccccgacaac taaaatagag
    26341 atgagtataa tccgaagaac taatgtattg ctttccttta aggaatatat taaagccgct
    26401 ttttcgtatt ctttcatgat ttaatccagt ctataagtat aagaaccaac aaaactgtcc
    26461 aggattcatt ttctgtgagc atatgaaatc ccatccagag tgatgatact ggcagaaaca
    26521 cgcccacagc cggaattatt agtctggcgg agaatatcct tatcaggagt atcaatagtg
    26581 ataagaacag aaaaagagtg atcatgtgga aaaacattca catcacctat aacactatta
    26641 agatagcctg ccagatatcc atttcagaca ggctatatac agggttcaat tcattctatg
    26701 aaaggtgtat agggattgct gatgtttgtg tcgatcaaaa atctaatatt aatagatgag
    26761 aactaatata catatttcca gacggatggg gggattttat cgtagagttt attcattgtg
    26821 agttctgcaa gcctgtgatc ggcggcggca ttaaacgact caagctcaga aggagggagg
    26881 ctgttttggt ttttttcaat aatcttttca agtgtgtcga gtgttgagca acgtcttaac
    26941 tgaaagagcc agtcttgttt tgttttcatt gtacaattcc tgaagaacat ccttgtccta
    27001 tagtagctac aacgatgttt gatttctact gctggattta tttcttactt ttttgtgtaa
    27061 actcaaatcc cagtttcccg gttgctttgt caaccagttt tacttttgct gaaaattctt
    27121 tgtttgtt
//
