LOCUS       NZ_CP030737             4119 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B2 plasmid pKpnB2_4, complete sequence.
ACCESSION   NZ_CP030737
VERSION     NZ_CP030737.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09519600
            Assembly: GCF_024585225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Johnning,A., Karami,N., Tang-Hallback,E., Muller,V., Westerlund,F.,
            Adlerberth,I. and Kristiansson,E.
  TITLE     Genome dynamics of NDM-producing Klebsiella pneumoniae isolated from
            a long-term colonised patient
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Johnning,A., Karami,N., Tang-Hallback,E., Muller,V., Westerlund,F.,
            Adlerberth,I. and Kristiansson,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2018) Department of Mathematical Sciences,
            Chalmers University of Technology, Department of Mathematical
            Sciences, Gothenburg SE-412 96, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JAN-2018
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Illumina MiSeq; Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:14:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,794
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,538
            CDSs (with protein)               :: 5,538
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 53 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 16 of 136
            Pseudo Genes (multiple problems)  :: 13 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030737.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B2"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKpnB2_4"
                     /geo_loc_name="Sweden: Gothenburg"
                     /lat_lon="57.6823 N 11.9613 E"
                     /collection_date="2013-04-17"
                     /collected_by="Clinical Microbiology Laboratory,
                     Sahlgrenska University Hospital"
     gene            complement(join(3874..4119,1..870))
                     /locus_tag="DR189_RS28835"
                     /old_locus_tag="DR189_29790"
     CDS             complement(join(3874..4119,1..870))
                     /locus_tag="DR189_RS28835"
                     /old_locus_tag="DR189_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_086540617.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LREDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(1215..1328)
                     /locus_tag="DR189_RS28985"
                     /pseudo=""
     CDS             complement(1215..1328)
                     /locus_tag="DR189_RS28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1745..1936
                     /locus_tag="DR189_RS28840"
                     /old_locus_tag="DR189_29800"
     CDS             1745..1936
                     /locus_tag="DR189_RS28840"
                     /old_locus_tag="DR189_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1936..2337
                     /locus_tag="DR189_RS28845"
                     /old_locus_tag="DR189_29805"
     CDS             1936..2337
                     /locus_tag="DR189_RS28845"
                     /old_locus_tag="DR189_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_117027411.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3242..3874)
                     /locus_tag="DR189_RS28850"
                     /old_locus_tag="DR189_29815"
     CDS             complement(3242..3874)
                     /locus_tag="DR189_RS28850"
                     /old_locus_tag="DR189_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_117027410.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKINNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFNEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(1216..1624)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 tattccgttc tcgatttcat caataagaat aattgagttt tcctgtacca gcactgagct
       61 gataaaagat aatgctcgac tgatgccctc gccaagcatt gatatttcat ttagctcact
      121 aaatccaatg tctaaatata tttccttatt attacctatc gcagatattg ctatatctac
      181 aattctgtca tctatttttt ttaagttttc tagtatgtct tttttccttt tttgtgttag
      241 taggctagat acatttgtaa ttgttgcctc tttgtttatc tttctggatg ttgttattat
      301 tgttgctgtc ttaaatttat aattaatccc atccctttcg ttttttattt cggattttat
      361 ttctacgcca tcttctctca attgcataga gaaaagctta ttggaaaccc tgtttttcgc
      421 attgggtgtc tcggtcacat ttatttttaa agagtttact gtggcctgag aaccgatagt
      481 ttgacgaggt atgttttggt tagatagtac ggtgtcaacg tttagagata aacttgattc
      541 taatctctga tttgtttctg tttcatatgt ttgtgttact tcagagcgtt catctcgaat
      601 gactatggac atcttttgtg atgtgtccat atcatgaaag taactaaccc aaaatggctt
      661 gtttttgttt atttctgcca tttcttttct gaatgcttga atgtttaaca aaacagccgg
      721 attttttaca tcataaaaac aaaatatagc atccagtata gatgttttcc cgtagttgtt
      781 tttcccagct atgatattta aagtctttaa ttgctttaga tatagctcat tgaagcattt
      841 gaagttcttt aaacttattt cctttatcat gatagcctct gttcgtgttt ttgttatatg
      901 ccatacgatt tgttagtacc ttaataaaat gtacagcttt cattgtgatt agctagactt
      961 tatgtcaaag attaactttg attgcttgtg ttttaatcga ttttttttac tttcttcttg
     1021 agattatctg gcagcagccg taagcttccg gctgtgaacg aaaaaaaccg ccttggcggg
     1081 cggttttttc gaaaggttca gtagagttgg cgctctatga accgaggtaa ctggcttaaa
     1141 gggagcgcag tcaccaaaac tgttcgttta gtttagccct aactggcgta taacttcaag
     1201 actaactccc taaatcagtt accagtggct gctgccagtg gcgctttgtc gtgtctttcc
     1261 gggttggact caagacgata gttaccggat taggcgcagc agtcggactg aacggggggt
     1321 tcgtgcatac agtccagctt ggagcgaact gccttcccgg aactgagtgt caggcgtgga
     1381 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1441 agagcgcacg agggagccat cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1501 tcgccaccac tgatttgagc gtcagattct gtgatgcttg tcaggggggc ggagcctatg
     1561 gaaaaaaggg ccgccttgcg gcctttcctt ttccccgtta cgcgaccgct cggttttctc
     1621 gagcttttat acgcatgctc ctgccgctcg ccgcagccga acggcaggag catagcgact
     1681 gagtgagcga ggaagcggaa aagagaacat ccctacactg agcactgacg ctctgaggcc
     1741 tcttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc
     1801 tctgctcctg ctggagaagc ttaacgagct tgatctcgat gtcgaagccg acctttgtga
     1861 gaaactccac gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt
     1921 acaggatggt aactgatggc cagagtgtct attagtgagg ctgcccgact gacgggtaag
     1981 agtagaacaa cactgcacag gttaattaaa gcaggtgacc tgtccacctg tagcggtgaa
     2041 cgcaatgcaa aaatggtaga tacttcagag cttttacgtg tctttggtcc ctttgaacaa
     2101 ccgaagggtg aacaccattc tggacaggtc tctgaacagc gtgatacagc ctctgctgac
     2161 cagagtgaac aggtgattcg tcatctgaaa caggagattg aacacctgaa caccttactt
     2221 ttgtccaagg attcgcacat tgacagcctg aaacaggcca tgctccttct tgaaaatcac
     2281 caggcaaaat taactgaatc cccgacgcca tggtggaaat tctggaaaaa atcctgaccc
     2341 cgtcatgctt cagttttcct ctccggccat cccggggagt tcctgaacct tcccaaaaat
     2401 cgtcagatct ctgaacgctc ctcaccacta aaaataccca caccccacgc aaaattaaaa
     2461 ttttgcagat tccttattta caacaatggg ttaatgaaaa ataaatgtgc ggtgatgtgt
     2521 tacaaagtgc agatccaagt cgtggacaac tctatggcga atcgctgacg aattgcccac
     2581 gattcatatg tgaatcacgc tttattatca tattgttatg atgaatcgac tcttgattca
     2641 attcatcact ctgggtgttt tttgttcatg gtgactcttg attcaattca cgattcgaat
     2701 cgccggtgaa ttgtctggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc
     2761 gacgaacccg attcctgatc gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc
     2821 acggccacgc aataaattgc gtccccgttc accgttttcg gataggaaga acgccggaaa
     2881 caggacttac cctggctgaa tggcgtgaat tttcgcgcta tgcacttgcg tgcatactca
     2941 tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct
     3001 gttttaccgg tttcccgtca gaaacgccct gaggccgttt tagcggtgcg tacaattatg
     3061 attttatgtt aaattgtttt aagtgaatag agagaatata acttaaggga gcatctggtg
     3121 atgtctttag gacgtttgag attgctgata actaaagtgt atgatgcttt ctatatgtta
     3181 tccggcgttt aataacaccc tggtgataag ccagcctgtg aagggctggc ttgatttgga
     3241 tttatatttt tgaaataaaa tcttttattt cattaaaaca attgtctgct gtgtttatta
     3301 ttccattttt catggatatt cctattcttg agtcggattc tctattgcag ctaagataga
     3361 cttgtacctt agcttttttt tcgtttgttg gataagtgta ctctttgtca ctcgctgaga
     3421 tgtcatcttt attattatta attttttcga aaaattctat tatgtattca tataacgctc
     3481 gagtgcttgc gtgttcaatt atcagatctt caattgcacc ttgcttgtct tttcctggca
     3541 ttataaagaa agaaattttt gtattgccat tcatagctat ctcgttatgc gagctggggg
     3601 aggagatttg tagttctcgc aatgtggcaa ttattgatct ttctgtgtca gaaaatgatg
     3661 tgtcagcatc taatataata aaaatacttt ctttttcatc gaaagaatca atgtttttga
     3721 atgctctaag tgcttgtttt aattttgatt ttccatttat tttttcgaca aaaacatcgg
     3781 tgatgccttg tatttcaagt aggttgcata gaaaatgaaa atcatcatca ccctcaacga
     3841 aaatgatctt tttgctatta tttatgtctt tcattatctt acctcccagt cgttctcaac
     3901 tgagtaggag aaatcatcca tattgaattg tacggctgtt ggttttaggg agttcttttc
     3961 tcttcctagt ctaatataag cgatatcttc atttttgcta tctatttcgt ttattgctcg
     4021 tatcacatct tgactatgag ttgttgcaaa tatttgattg ttgttttgtt ttgcagatga
     4081 aattaatgat tttattatgt ctttaattac tgagtaatg
//
