LOCUS       NZ_CP030346             4858 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain AR_038 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP030346
VERSION     NZ_CP030346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014879
            Assembly: GCF_003288475.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_003288475.1
            Current Name                      :: Enterobacter hormaechei
            Previous Name                     :: Enterobacter cloacae
            Date Updated                      :: 2019-03-07
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001729725.1
            A1 Name                           :: Enterobacter hormaechei
            A1 ANI                            :: 99.04115%
            A1 Query Coverage                 :: 81.64%
            A1 Subject Coverage               :: 90.24%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000025565.1
            A2 Name                           :: Enterobacter cloacae
            A2 ANI                            :: 87.62986%
            A2 Query Coverage                 :: 75%
            A2 Subject Coverage               :: 70.81%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 13.96x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003288475.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 02:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,217
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 46 of 125
            Pseudo Genes (incomplete)         :: 74 of 125
            Pseudo Genes (internal stop)      :: 16 of 125
            Pseudo Genes (multiple problems)  :: 10 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030346.1.
            The organism was changed from 'Enterobacter cloacae' to
            'Enterobacter hormaechei' in March 2019.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="AR_038"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
     gene            339..830
                     /locus_tag="CSB67_RS00955"
     CDS             339..830
                     /locus_tag="CSB67_RS00955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(887..1315)
                     /locus_tag="CSB67_RS00960"
     CDS             complement(887..1315)
                     /locus_tag="CSB67_RS00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            1600..2028
                     /locus_tag="CSB67_RS00965"
     CDS             1600..2028
                     /locus_tag="CSB67_RS00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            3011..3334
                     /locus_tag="CSB67_RS00990"
                     /old_locus_tag="CSB67_0005"
     CDS             3011..3334
                     /locus_tag="CSB67_RS00990"
                     /old_locus_tag="CSB67_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3543..>3977
                     /locus_tag="CSB67_RS27060"
                     /pseudo=""
     CDS             3543..>3977
                     /locus_tag="CSB67_RS27060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4012..4503
                     /locus_tag="CSB67_RS26880"
     CDS             4012..4503
                     /locus_tag="CSB67_RS26880"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4510..4725
                     /locus_tag="CSB67_RS26885"
     CDS             4510..4725
                     /locus_tag="CSB67_RS26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2725..2898
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(2256..2801)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          3325..4817
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatttctc ccgctaaata ggttcgtgat caaaaaggtc acgacttatt caggagcggg
       61 aaatgaagct gttaaatctt attgaatcat tgcttaactg cctgtatctg gcggcggagc
      121 tgtgggactg gctggagaag catttgtcgg tttttaaggc attggcgcgg attttagtgt
      181 gactttaaca tccatgcatc tggctgcatg actatgcacc agtgtaatat tcagcggctg
      241 cgtttctggt gttttttcgt tatgtctgtc gccactttct cgcgtctggc accggaaacg
      301 cagccaaccc cgtttcagcg gtgcgggcaa cagatgttat ggtaaatcaa ccgtccgttg
      361 ataaaggttc tgataatacc catgtaggtg atgttgtttg ctttttatct acacactgcc
      421 tgatggagct atttatgaat gctgatgaag ttacacgaat tcttgcgcaa tcgagtcaaa
      481 atgattggat tgtggatgat gagacagggg cgtttactta taaaaatgat ttagcactgc
      541 acattaagag agctgattac gacgactaca actcgtttaa tgagccttgg gctgttcgtc
      601 atcctgatag taatgcgtgt tcagtcgagt acaccgttca ctatggttct tcgtttgttg
      661 atagaaaaac cttggtttcc gtcgatggtc atcgtgcaac cttgcctatg cctgcatccg
      721 caacggatct aaacgtgtca tcggctgatg tgaatttcgc caaaattgta aacattggcg
      781 atcgcgttga tgaatacatt caacgttctg gtcttacgat taagccctaa tttaacgata
      841 agcccggcct ccaaatgcgg gctgttcctg catgtaacca gcataactaa tcggcttttt
      901 tccttactgt atatattggc tttttatgtc gcggctttct tacttgctcg gacacagaag
      961 gaccaattat gtctaaataa tctacaagct ttgtaaggtc gtatatgttg cggccaccat
     1021 atcggttgtc gctttttgat gtcggttttt tatctaagag attggctttt tctaggtcat
     1081 taatagccct ttgcaccgtc cttactgaca cccccatccg tatagccagg ctctccattg
     1141 aggggtgcgg ccactcctgt agattccacc agtgtactat gagattcagc aaggtattga
     1201 atgctacagg gctcaatttc ttctcatttt gtataaaaaa caacacagta ggtactgcag
     1261 tccatcctaa tgacagacct ccagccttcc attttttttc tattgcgtaa ctcattgatt
     1321 atcctgtaaa tatttttttt gagatatata taatataagt attcatatga atagggtgac
     1381 accagcgcca ttattacaca actataaaag gctaatctga gaacagaaaa acatttctat
     1441 ctgcattaaa tacggagtta gtgatatgaa aaccaaacaa catcatgatc ttgtaaaaga
     1501 tactgaaata tttggtgtat ccttgtctct gttgatttcc agagcgaatg agagatctgt
     1561 tacaatgcca tctggtctaa gtagggagca gagaagagca tgggcagcgg atcaagcacg
     1621 caaaatccac aattgaatgt tgtctctttc tctgagacat tcaatcttta ttacacatcc
     1681 cgcttttctc aaaaaacact cgataaaatc gatgacttca tcgaacattt cgagaagtac
     1741 aatctttttg gcgatgcctt aaaaggccat ccggcttgga ttggcaaggt atcgccatct
     1801 tggaacgtgc ctgacagtta cgaaaacaaa gaagctattg aagaacatgc cagggctaat
     1861 aaattgtggc atgcccatat aggcgatccc gttttcatcg atacatttca cgggaagtac
     1921 aaagtctctg attgggtcat ccacttccaa cgtctagcgc cgaaccatat aaaactgtta
     1981 gagcttgggt atcatgaccc tatgcaactc ccccaaaatt tcgaatgatc tcttgttcgg
     2041 atgaatgatc ccttgagatt cgttgaccct cgtcgtaatc tcttgcggtg aaaacgaaaa
     2101 aaccacccgg caaggtggct ttttcgaagg ttcgcaagag ttgacgctct tttgaaccgc
     2161 ggtaactggc ttggaggagc acagtcacca aaactgtcct ttcagtgtag ccttatcgca
     2221 cccgaaactt caagactctc ctcctctaaa cctgttacca gtggctgctg ccagtggcgc
     2281 tttgtcgtgt ttttccggat tggactcaag ttgatagtta ccggataagg cgcagcggtc
     2341 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaact
     2401 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     2461 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
     2521 aagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg
     2581 ggggcggagc ctatggaaaa acggcttcgc cgcggcctta tcgtttccct gttaagtttc
     2641 ttcctggcat ctaccaggaa tttccgcccc attcgtaagc ccacaccgct cgccgcagcc
     2701 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcgcatat
     2761 tctgctgacg cgccggtgcc gccttttttc tcctgccaca tgaagcactt cactgattcc
     2821 cgcacccgtg ccaatataat aagccagtat acactccgct agcgctacgt gactggttca
     2881 gggctgcgcc ccgaaacccg ccaaaagcct gacgcgcctg cggcttgtcc aaccccgcgc
     2941 cgaccgggaa aggctttccc gcccgtcccg gcgttatcag gaggccggtt tgtcagagaa
     3001 acggagcaag atgctcacca tgtgggtgac ggaggacgag caccggcggc tgcttgagcg
     3061 ctgtgacggc aggcagctgg cggcgtggat gcggcagatc tgcctggacg aaaagccggc
     3121 acgttccgga aagctcccct cgatctcgcc ggcgctgctg cgccagcttg ccggcatggg
     3181 caataacctg aaccagatag cccgccgtgt taacgccggc ggcggcaccg gccatgacag
     3241 ggtacagatt gtggcggcgc tgatggccat cgatgccggc cttgagcggc tgcggcacgc
     3301 cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccgcgcggc cgcggcggtg
     3361 gcgccgggcc ggtggactat ctgctgggta aagaccggca gcgtgaaggc gccaccgtcc
     3421 tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac gttaagaaat
     3481 acacctccgg cgttctgtcg tttgcggagg ccgatctgcc tcccggccag cgggaaaaac
     3541 tgatggccag cttcgagcgg gttctgatgc ccggactcga taaagatcag tacagcattc
     3601 tgtgggtgga gcacgcggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg
     3661 agctgctgac cggcaggcgg ctccagcctt actacgaccg cgccgaccgg ccgcgcatcg
     3721 atgcctggca gactatcgtg aacggcagac tggggctgca cgacccgaac gcgccggaaa
     3781 accgccgggc gctggtcacg ccgtccgggc tgccgaaaac gaagcaggag gccgcagaag
     3841 ccattacccg gggactgctg acccttgctt cgtccgggga gctgaaaagc cgtcaggatg
     3901 tcactgacac tcttgaaggt gcaggttttg aggtggtgcg caccacgaaa agcagtatca
     3961 gcattgccga cacggacggg gggcgaaaca tccgactgaa gggagccatc tatgagcagt
     4021 cttttaacgc tggcgaagga cttagagcag caatcgaaag cgcagcagca gagtaccggc
     4081 gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgagcgca
     4141 agcgcgagga gaatcagcga cgccatcctc gcccacgagc agagtatgag cgaggcgatg
     4201 gagaaaaacc ggcggagcgt actgcggact gccgggcgga catggctcac catcctgatg
     4261 gtgtcagcgc tgctgatcgg cacgagcggg agcattctgt ggtggcaggg gcagcagata
     4321 accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg
     4381 acgtggggag tgcgctatca ggagagcagc gacggccgga ggtttcttat cctgccgccg
     4441 gggatgcaaa ccgaagccat cccctacgac gggacgacat ggatccgcct gaaacaggag
     4501 tagcggaaaa tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag
     4561 gactactcgc aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt
     4621 ggtcttatgc aacgggagaa cgcgatgctg aacgagcgcg tgtcggactt gagcacgcag
     4681 gtgctgagtt taagcgagca gctgcgccgc ttgtccggga actgaatgcc atcgagctcc
     4741 ggcagaggga gaatgagtat cagaaaacac tggagctgga gcgccagccg cagaaaacct
     4801 accgcgggcc gacactttaa accatccagg cagctgacaa cccgcacgaa gaggggct
//
