LOCUS       NZ_CP030344             8667 bp    DNA     linear   CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain AR_362 plasmid unnamed3.
ACCESSION   NZ_CP030344
VERSION     NZ_CP030344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291505
            Assembly: GCF_003288375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0; HGAP v. 3.0
            Genome Coverage                   :: 11.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:48:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,711
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,472
            CDSs (with protein)               :: 5,472
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 53 of 115
            Pseudo Genes (incomplete)         :: 68 of 115
            Pseudo Genes (internal stop)      :: 15 of 115
            Pseudo Genes (multiple problems)  :: 18 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AR_362"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
     gene            87..323
                     /locus_tag="CSB99_RS29285"
     CDS             87..323
                     /locus_tag="CSB99_RS29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000792381.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRGL
                     NAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
                     /name="hypothetical protein"
     gene            383..934
                     /locus_tag="CSB99_RS29290"
     CDS             383..934
                     /locus_tag="CSB99_RS29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000662998.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYRIKLTSPDF
                     KNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALDNV
                     DMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDGYEM
                     VIRGLSVDQLIKVAGFIKQL"
                     /name="hypothetical protein"
     gene            943..1212
                     /locus_tag="CSB99_RS29295"
     CDS             943..1212
                     /locus_tag="CSB99_RS29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001006717.1"
                     /translation="MNEVLSDRKNKGNVTYRIVVSEDATRLFIELEKLMKVRSKEADKK
                     TTKKIVFQKSFYYEEFSNVKDEIDDPILLKFYTDTIVKVQNHEF"
                     /name="hypothetical protein"
     gene            1202..1444
                     /locus_tag="CSB99_RS29300"
     CDS             1202..1444
                     /locus_tag="CSB99_RS29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000008707.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKTS
                     RGLAAGTYELLLLLADEHPDYQLVKREKNQDKVTK"
                     /name="hypothetical protein"
     gene            1538..1876
                     /locus_tag="CSB99_RS29305"
     CDS             1538..1876
                     /locus_tag="CSB99_RS29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001099725.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRGY
                     VITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEAKN
                     EAIRELRS"
                     /name="hypothetical protein"
     gene            1951..2115
                     /locus_tag="CSB99_RS30895"
     CDS             1951..2115
                     /locus_tag="CSB99_RS30895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000004083.1"
                     /translation="MSERMLSAIQTVEKGGRPVFPLMPFSAFPEYMALLRKALEKKETK
                     ALIEKQEVL"
                     /name="hypothetical protein"
     gene            2112..2369
                     /locus_tag="CSB99_RS30655"
     CDS             2112..2369
                     /locus_tag="CSB99_RS30655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004199355.1"
                     /translation="MKKQEFLDFISAEQRRGAVRFSLGFNSKGEIVLHWTNEAGLRVWS
                     ILSGNRGKSPSRANRERMSNLRRWLHDARQGMEGDTPEAE"
                     /name="hypothetical protein"
     gene            2710..3255
                     /gene="ddp1"
                     /locus_tag="CSB99_RS29315"
                     /old_locus_tag="CSB99_5794"
     CDS             2710..3255
                     /gene="ddp1"
                     /locus_tag="CSB99_RS29315"
                     /old_locus_tag="CSB99_5794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_001348698.1"
                     /translation="MKKVQFRIEESQHDDLLDCLKTLYPDEPGLTVAKGMKLLANALLK
                     SKVKNEDVNVSKDNDDFIKTTMYLTGKQRTLIEKAAHRHGWNLSRECRYRIQTTLENEL
                     DFFDQELLVMNRCRNAIDKIGRNFHYIIVNDNARVLDKDGFYQDAERLSSEIMSLKSEF
                     ENYIMLCKGRTVSNKVEV"
                     /name="ddp1"
     gene            3258..4418
                     /gene="mobP1"
                     /locus_tag="CSB99_RS29320"
                     /old_locus_tag="CSB99_5795"
     CDS             3258..4418
                     /gene="mobP1"
                     /locus_tag="CSB99_RS29320"
                     /old_locus_tag="CSB99_5795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP1 family relaxase"
                     /protein_id="WP_004199101.1"
                     /translation="MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINR
                     KGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWKGDEIQEAKEHMIDR
                     ANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPEDLLESVRKTMNKKYPNHRFVLGYH
                     NDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYDVKATHKQQHGLN
                     QSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYWGADFG
                     ELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGVKGIDRI
                     SSSSKELVLNSAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG"
                     /name="mobP1"
     gene            4771..5280
                     /locus_tag="CSB99_RS29325"
                     /old_locus_tag="CSB99_5796"
     CDS             4771..5280
                     /locus_tag="CSB99_RS29325"
                     /old_locus_tag="CSB99_5796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /GO_process="GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0140673 -
                     transcription elongation-coupled chromatin remodeling
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_000455437.1"
                     /translation="MEWYVLQFTTTRFAAVFAHLERLNFSYFCPMETERYRRPDKIISY
                     RERRLPLFPGYLFIQADFEEVHSTTITAIPYVQRFISFGGEPLPVPEDVMAELLYRQSH
                     TTAQANLLRKSIPHDFAEILLMDNPQQRSMAFIHYITERSLTHKMKRKKNDCYPKKNRN
                     ETQAPT"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            5513..6157
                     /locus_tag="CSB99_RS29335"
                     /old_locus_tag="CSB99_5797"
     CDS             5513..6157
                     /locus_tag="CSB99_RS29335"
                     /old_locus_tag="CSB99_5797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000953538.1"
                     /translation="MLSTTAFAALALQCAASVHPDTAHEVARVESGFNPYAIAEIIPKV
                     ERKPGDKGVVSYFPKTKEAALQIVNQIESRNHRYSVGLMQITSTNFANFNTTAEKMFDP
                     CENLKVSEQILVDCYKRGGDILRGLSCYYSGNPETGTKPESDFNNTSYIQRIGFNPPDN
                     KKNWVVPSVKDAIRKENVTQSIKPKEVTVYPQYAMRGTVLDEKETNDVKTQ"
                     /name="lytic transglycosylase domain-containing protein"
     gene            6141..6431
                     /locus_tag="CSB99_RS29340"
     CDS             6141..6431
                     /locus_tag="CSB99_RS29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000916156.1"
                     /translation="MLKLNKRYLTLSVFMAALMLCVAEPAFADDVSTKTTGFLQKIIDF
                     LTDIRKPAITIIALVIGYIAIFSRQHAAWITPLIIGIIIFIVAPYLPDWLA"
                     /name="TrbC/VirB2 family protein"
     gene            6456..>8667
                     /locus_tag="CSB99_RS29345"
                     /old_locus_tag="CSB99_5799"
     CDS             6456..>8667
                     /locus_tag="CSB99_RS29345"
                     /old_locus_tag="CSB99_5799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_112295953.1"
                     /translation="MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYAL
                     ILPGWYAIKRVTKIDERFFDLLYLRMQIKGNPLANKRFNAVHYAGSSYDAVDISKVDNF
                     MKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQIDGAYFECVDEADLALLTDQLNT
                     LIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSAAEYFENKLYLTV
                     CYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLYEENNI
                     VYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARYFRCIEI
                     KDYFQETDAGIFDALMYLPVEYVQTSSFTPIDKQSAIKALDDQIDKLEMTDDAAKSLLA
                     DLKVGLDMVSSGYISFGKSHQTLIVYADSPERLVKDTNIVTTTLEDLGLIVTYSTLSLG
                     AAYFAQLPGNYTLRPRLSSISSLNFAEMESFHNFFTGKEKGNTWGNNLITLGGSGNDIY
                     NLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFAAQQFGTPESFPPERKL
                     KKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNISFMKDLVRLLC
                     TLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTETRRHGLKARLNAW
                     VQGGEFGWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAAS"
                     /name="VirB3 family type IV secretion system protein"
     Typing          3259..4415
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggacacgt gtagaagcaa gcgcataacc ggaatggggg cgaaagcccc ctagaacatg
       61 gcaaagattg attcggagac acgttgatga aaaacaagac cataacggaa gcggagctga
      121 taaacatttt tgaaagttac ggagcctaca tttgcccgga tgagatcgaa gttacagcca
      181 aagaatgcaa tgaaaatggt tcagtcttgc accgtggctt aaacgcagaa ggatgggcgc
      241 atctttttgc gaaggaagaa gcatatcagc aggaatgcga agcccaggaa gctgcaagtg
      301 atgatggaca ctttgatgaa tagtcttaaa actgccgtaa ttcgatttac ggctttttta
      361 ttgagttcaa aaggctgaat cgatgaaaga acagaaaaat ttctttgaac ggtataaacc
      421 ggtgtttgaa atcgtttgtc gaatcctggg gaacggctgg cgtgtaaatc tgctcgatga
      481 ttgccagtac aggattaagc taacctcacc tgattttaaa aattactcta tacatattcg
      541 aatggagaaa ggaaggttag ttatcattgg aagcgtagat agtcgcagct ggagaagtcc
      601 ttaccatact tgcacagtat cgccagagcg taaccctgta gaaatcgccg ccgatattga
      661 aaagaaaatc ctgtcggatg ctttagataa tgtagatatg gcgcgagagt atgaacaaca
      721 gttacagcaa aaacgcgaga aaaaactgat cctgaaagga atgctatctc gtttagtgca
      781 tcttgaaagc tggcatggga cattgaccgg gtttaaagtt gagaatgggt tagatggtaa
      841 cgtatcagaa cggggcgacg gatatgaaat ggtaattcgt ggcctgagtg tagatcagct
      901 cataaaggta gcggggttta ttaaacagct atgaggcaaa aaatgaatga agtcctgagc
      961 gacaggaaaa acaaaggtaa tgtaacatac aggattgttg tgtctgaaga tgcaacacgt
     1021 ttgtttattg aacttgaaaa actgatgaag gtacgctcta aagaagcgga taaaaaaaca
     1081 actaaaaaga ttgttttcca aaaatcattt tattacgaag aattctcgaa cgttaaagat
     1141 gaaattgatg atccaatttt attgaaattc tatacagata ctattgtgaa ggtacaaaat
     1201 catgagtttt aagccaacac ctgaagaggt gaaacaagca cgtattaaag ctggctttac
     1261 gcagcaggaa gccgctgaac ggtttggttt tacgttatca gcctggcaag ccaaagaaac
     1321 atccggtaaa acgtctcgtg gtttagccgc tggtacgtat gaattacttt tgctcctggc
     1381 tgatgaacat ccagattacc agctcgttaa gagagaaaaa aatcaggata aagtgactaa
     1441 ataaattgca ttaccatcat atgatggtat aatttatttg taggtcggga atagttcctt
     1501 accatgatcc agtagcccaa acgggcggga gactaaaatg aacacgtatg ttatcacttt
     1561 ccaggcttta aacactaaga aagaaaaaat cacctgctct gcttttgtcc attcagaaac
     1621 tctcttcctt gccgttcatt ctttcgaaga taaaaatcgc ggtcgtggtt atgttatcac
     1681 gtctgttagt gaattgttgt ccgaggaact gacggcgaaa aattccctca agaaaaatat
     1741 taacttttgg tttaatgagt gtggtttaag taaatctgag gttataaacg aagtaattaa
     1801 ctggaagaat ttcgcatata cccttaaaga actggaggaa gccaaaaatg aagcgataag
     1861 agaactgcgg agttaatcaa ttaggggtgg tatcaggttg tggaggcaac ctgataccgt
     1921 gacactcaat tccctcgcga ggaatataaa atgtcggaac gcatgttatc agcaatacag
     1981 actgttgaaa agggtgggcg tccggttttt cccttgatgc cattctctgc ttttcctgag
     2041 tacatggcat tactcagaaa agccctggaa aagaaagaaa caaaagcact gatagaaaaa
     2101 caggaggtgc tatgaaaaaa caggagtttt tagattttat cagtgcagaa cagaggcggg
     2161 gagctgtccg gttttcgctg ggatttaaca gcaaagggga aattgtattg cactggacta
     2221 acgaggccgg gttaagagtc tggagtatac taagcggtaa caggggaaaa agtcccagcc
     2281 gggcaaaccg ggaaagaatg agtaacctcc gccgctggct ccatgatgcc cggcagggca
     2341 tggaaggcga cacaccagag gcagaataaa attcccgttc ctgaatcgcc gaagccctcc
     2401 cgtttacggg aggtgctttt tttgtatatt ccgtaaaaca gcgctcgcgc cccctgccag
     2461 gccattcgcg tagcgaattt atttccgccc aagttagccg cgaatccgtt gtctccggtc
     2521 gcagacgcac cagatcacag ccacgatagc ctttcatttt ttgcatttcg atggacgctg
     2581 aagcacaatt tatgtgtgga ggtagttcac tgcgatgcag gatagggcaa acgccgcaaa
     2641 atgacgtctc tgacgccatt ccgcaacgtt tgcccggtga cctacagtcg gttcttgttg
     2701 gagagtttta tgaaaaaagt tcaattcagg attgaagaaa gccaacatga tgatctactt
     2761 gattgtctta aaacacttta tccagatgaa ccgggtttaa ccgtggcaaa aggaatgaag
     2821 ctgcttgcca atgctttact gaaaagcaag gttaaaaatg aagatgttaa tgtgtcaaaa
     2881 gacaatgatg attttatcaa aaccacaatg tatttgacag ggaaacaaag aactttaata
     2941 gagaaagcgg ctcatcgtca tggctggaat ttatccagag aatgtcgtta tcgaatccag
     3001 acgacacttg aaaatgaact tgattttttc gatcaggaac ttctggtgat gaatcgttgc
     3061 cgtaatgcaa tagataaaat agggcgaaac tttcattata ttatcgttaa tgataatgcc
     3121 agagtacttg ataaagatgg gttctatcag gacgctgagc ggttgagttc agagataatg
     3181 agtctcaaaa gtgagtttga aaactacatt atgttatgca aagggcgaac tgtttctaac
     3241 aaggttgagg tgtaatcatg ggcgtttatg ttgacaagga attcagggtt aagcgtaagt
     3301 cctctgaagc tggccgcaag tctgctttcg cccataaagt taaaaatggt ggtaaaaact
     3361 atcaacgcaa cgttcaggaa cgtatcaacc gcaagggtgc cagtaaggag gttgttgtca
     3421 aaatctcagg aggtgcgatt acccggcagg gagttcgaaa cagtattgac tatatgagcc
     3481 gggagtcaga gctgccagtg atgagtgaaa gcggtcaggt atggaaaggt gatgagattc
     3541 aggaggctaa agagcacatg atagatcgtg ctaatgaccc tcaaaatgtt ttcgatgata
     3601 aaggtaagga aaataaaaaa gtaacccaaa atattgtgtt ttcaccgcct gtatcagcaa
     3661 aggtaaaacc tgaagatctg cttgagtctg tcaggaaaac gatgaacaag aagtacccaa
     3721 atcatcgttt tgtgcttggc tatcataacg acaaaaaaga acatccacac gtgcatgtcg
     3781 tttttcgtat tcgtgataat gacggtaaac gagctgatat caggaaaaaa gatttacggg
     3841 aaattcgtac aggtttttgt gaagagctga agatgaaggg ctatgacgtt aaagccactc
     3901 ataagcagca gcatggactt aaccagtcta ttaaagatgc gcacaaaaca gcgccaaaac
     3961 gacagaaggg cgtttatgag gttgttgatg ttggctacga ccattatcag aacgataaaa
     4021 ccaaaccaaa gcagcatttc ataaagctta agacactgaa caagggcgtg gagaaaacat
     4081 attggggggc tgattttggt gagttaacca ctcgcgagaa cgttaagaaa ggtgatcttg
     4141 ttaagctgaa gaagttagga caaaaggagg tgaaaatccc tgcacttgat aagaacggtg
     4201 tacagcatgg ctggaagact gcacacagga atgagtggca gctagagaac ctgggggtta
     4261 agggcataga cagaatctct tcatccagta aagagctggt gctgaacagc gcggagatga
     4321 ttaagaaaca gcaactccag atgagaaatt tctcgcaaat aaaacagtcg atgattcaaa
     4381 gcgaacagaa agtgaaaatc ggaattcgtt taggataatt catactctcc ggtaacttat
     4441 tgattaagat cgatttattt atcttgttag cagagttttt gtaaaaagta actagcggta
     4501 aaaaatttag ttaacttgac tataaaataa gcgcatggtg atcaatcgat ctgctttttt
     4561 gttatcaaat agcatatgta tctagatata aataaaatac aagcacaaac aaaatgaaat
     4621 gtgtcgtaaa tgcgatctaa agatctgctt gtgtggttga acgcagcaca tgtagctgat
     4681 aaagttttta tgagatgtaa atttgtcctt ttgttttttt tctctgattc ggggtcatcc
     4741 gagtcgagag agcgcctgaa aggaaaaact atggaatggt atgttttgca attcaccact
     4801 acgagatttg cagcagtttt tgctcatctc gaacgcctga acttctctta cttctgtcct
     4861 atggagactg aaaggtatcg tcgcccggat aagataattt catatcgaga aagacgtttg
     4921 ccgctttttc ccggttatct ttttatccag gcagattttg aagaggttca ttccactaca
     4981 ataactgcta tcccttacgt acaacgcttt atctcatttg gcggtgagcc tttacctgta
     5041 ccagaagatg tgatggcgga gcttttgtac agacaatcac atacaacagc tcaggcaaat
     5101 cttttaagga aatcaattcc acacgatttt gctgaaattc tactcatgga taatccgcag
     5161 caaagaagta tggctttcat ccactacatc acagagagaa gcttaaccca caaaatgaaa
     5221 cggaagaaaa atgactgcta cccgaaaaaa aaccgcaacg agacgcaagc cccaacgtaa
     5281 tacactctac ctgccctcag cttctcgtga ggaactggat aaaattgctc tggagattgg
     5341 ctatcgcagg gggcgacgta tttcaagctc tggtgttgtc cagtacctca taaaaaaata
     5401 ttccagccag gctattgagg agctgataaa cgaagacgat gaagacgatg acgacttcga
     5461 tgactaacca ttcagtctaa actttccgcg ctgttctggc cgggagaaaa tcatgttatc
     5521 tactactgct tttgcggcgc ttgctttgca atgcgcggcc agcgttcatc ccgatactgc
     5581 gcatgaagtt gcaagggttg aatctggttt taacccatat gctattgccg agataatacc
     5641 gaaggttgaa cgtaagcccg gtgacaaagg cgtagtgtcc tactttccca aaactaagga
     5701 ggctgcactc cagatcgtaa atcaaattga atcacgaaat catcgttact cagtcggtct
     5761 tatgcaaata acgagtacga attttgcaaa cttcaataca accgctgaga aaatgtttga
     5821 cccctgcgaa aatctcaagg tttcagagca aattttggtg gattgttata aacgtggtgg
     5881 cgatatttta cgtggcctga gttgctatta ttccggcaat ccagaaacag gaactaaacc
     5941 tgaatcagat tttaataaca ccagttatat acagcgcata gggtttaatc cgcctgataa
     6001 caaaaaaaac tgggtggttc cttcagttaa agatgcaatc agaaaggaga atgtaacaca
     6061 gagtatcaaa cctaaagaag ttaccgtata tccccaatat gccatgcgtg gcactgtttt
     6121 agatgaaaag gaaacaaacg atgttaaaac tcaataaacg ttatttaact ctttctgtat
     6181 ttatggctgc attgatgctt tgtgtagcag aacccgcctt tgctgatgat gtatccacta
     6241 aaacaactgg cttcttgcag aagattatcg attttctgac ggatatccgt aagccagcaa
     6301 tcaccatcat tgcgctggta attggttata tcgctatctt ctcacgccag catgcagcgt
     6361 ggattacgcc tttaattatc gggattatca tctttattgt tgcgccatat ctgcctgact
     6421 ggttagcgta atatgtaata aaagggggcg taaaaatgag taccgttttt aaagggctga
     6481 cacgccccgc tttgattagg gggctgggcg ttccgctcta cccctttctt ggaatgtgcg
     6541 ttatctgcgt tttgcttggt gtctggattc atgaggctat gtatgccctc atcctccctg
     6601 gctggtatgc catcaagcga gttacgaaga tagatgaacg cttttttgac ctgctttatc
     6661 tgcgaatgca aatcaaaggt aatcctctgg caaacaagcg cttcaatgcc gtccattatg
     6721 cggggagttc gtacgacgca gtcgatatat cgaaagtgga caattttatg aagcttaaag
     6781 accagtcttc tcttgaagag ttaatcccgt attcatcaca catcactgat aacctcattg
     6841 ttaccagaaa ccatgatcta ctggcgacct ggcagattga cggagcttat tttgaatgtg
     6901 tagatgaagc ggatctagcc ttgcttactg accagctcaa tacgctgata cgtagttttg
     6961 atgggaaacc cgttaccttt tatacgcatc gtattcgggt tagaaaagaa gtgcgacctg
     7021 tatttgacag caagatccca ttcgtcaaca gagtgatgaa cgattattac gaatcgcttt
     7081 cggcggctga gtattttgaa aacaaattat acttaaccgt atgctataaa ccgttcagcg
     7141 ctgaagataa agtgacgcat tttctttcaa ggaaaaaggg taataaaaat atcttcgaag
     7201 aacccattaa tgatatgaat gaaatatgtg gcagattgag cacctatctc tcccggtttc
     7261 attcccgtcg tcttgggtta tatgaagaaa ataatatagt ttattcagaa cagcttacgc
     7321 tgttccagaa actgctatcc gggcgctggc agaaagtcag agtcaccaat agtccgtttt
     7381 atacatacct gggtggtaaa gacctgttct ttggtaacga tgctggacaa atcactgcct
     7441 ctgaccatgc ccggtacttt cgttgcatcg agatcaagga ctactttcag gaaacggatg
     7501 ccggaatttt tgatgcactg atgtatctcc cggttgagta tgtacagact tcctctttta
     7561 ctccgataga caagcagtct gcaatcaagg cgctggatga tcagatcgac aaactcgaaa
     7621 tgaccgatga tgccgctaaa tccttgctcg ctgatttgaa agttggtctc gatatggtct
     7681 ccagcggcta tatttctttc gggaaaagtc accagacact gattgtttac gcagattcgc
     7741 cggagcgtct ggtgaaggac accaatattg taacgacgac cctggaggat ttagggctga
     7801 tcgtaaccta ttcaaccttg agtcttggcg cagcgtattt tgctcagtta cctggtaact
     7861 acacgttacg gccacgcctg agttctatca gcagtctgaa ctttgccgaa atggaaagct
     7921 tccataattt ctttacgggc aaggagaaag gaaatacctg gggtaacaac cttatcaccc
     7981 tgggcggctc aggaaatgat atttataacc tgaactatca catgactacc gagcatcaaa
     8041 attacttcgg taaaaacccc acgctggggc ataccgaaat tcttggtacg tcaaacgtgg
     8101 gtaaaacggt agtgatgatg actaaggctt tcgccgccca gcagttcgga acgccggagt
     8161 cttttccacc tgagaggaaa ttaaagaagt taactacagt atttttcgac aaagatcggg
     8221 cagcggaacc cggtatacgt tcaatgggag gtgcttactt tcgggtgaaa gaaggagagc
     8281 cgactggctg gaaccctgcg gctttgccac caaccaaacg aaatatttcc tttatgaaag
     8341 acctggtgag gctactttgt acgcttaaca gtgagccgct tgatgattac cagaaccgcc
     8401 tgatttctga tgccgttgag cgcctgatgc agaggtcaaa ccgctcttat ccgatcagta
     8461 agttgcgacc actcattctg gagcctgatg atactgaaac ccggcggcat gggcttaaag
     8521 cacgcctgaa tgcctgggtg cagggagggg aatttggctg ggtgtttgat aacccggacg
     8581 atacgtttga tgtggacaac ctggacgttt tcggtataga cggaacggag tttctggata
     8641 acaaagtact ttccagcgcc gcttcat
//
