LOCUS       NZ_CP030338             1549 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_451 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP030338
VERSION     NZ_CP030338.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291544
            Assembly: GCF_003288455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 10.03x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003288455.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 02:15:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,980
            Genes (coding)                    :: 4,764
            CDSs (with protein)               :: 4,764
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 78 of 216
            Pseudo Genes (incomplete)         :: 153 of 216
            Pseudo Genes (internal stop)      :: 39 of 216
            Pseudo Genes (multiple problems)  :: 46 of 216
            Pseudo Genes (short protein)      :: 1 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030338.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_451"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
     gene            438..914
                     /locus_tag="CSC38_RS26585"
                     /old_locus_tag="CSC38_0015"
     CDS             438..914
                     /locus_tag="CSC38_RS26585"
                     /old_locus_tag="CSC38_0015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1359..1484)
                     /locus_tag="CSC38_RS26595"
                     /old_locus_tag="CSC38_0017"
     CDS             complement(1359..1484)
                     /locus_tag="CSC38_RS26595"
                     /old_locus_tag="CSC38_0017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     Typing          complement(251..359)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          complement(1146..1406)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 gtgcatgcgc ggttatacga ttacacatcc tgtcccgatt tctgaggcgt tttaacggtg
       61 aacggacggc aaaaacacga tggagctgtt gaacgtgaaa accacgaaat ctggcaaaaa
      121 tcacggctgg cgaggcttgc ataggagtaa gccagtatac actccgctat cgctacgtga
      181 ctgggtcagg gcttcgcccc gacaccccca aagggcgttg cgttgcacgc aacacccttg
      241 cctagaatag atcttttacg ccgatttgta agtggttgtt tttttagtgt gttttttatt
      301 tacctgtcgc atccaggatg acacaacgtt gtcgcatcca ggatgcgaca acacgcaaca
      361 aggtgttgca cagatgtgtc atgatggctt agatttaaac caacgttagt gcatgggatt
      421 ttcagaggga aaaaatcatg tttattgatt cagaaaaacg actgaaacaa ctttcagatg
      481 aggcaaagaa aaacaccgag gatctcgaag aagcaaagaa aaattcaagg tttacacagg
      541 tatccccaaa aggttgggaa cgtgttcgag agctgctgaa ggatagccaa ggcatatcag
      601 cactgaagct gtactcattt ttagcggagc atatcgatcc tacgtgtggc gctgtcgttg
      661 cggatcagca attcctagct gaaaaacttg gagttagcag aagcacaatt attcggtggc
      721 tcaattactt agaatcaaaa aatgcattag ttagaatccc cgttgctggt aaggtttgtg
      781 cgtatgccct cgatccacat gaagtctgga agggatacaa cactacgaaa aaccatgcag
      841 cgtttgtcac taaaacactg gtcaacaaag acggtgatat tcagcgccga atcatggcca
      901 tgttttcaaa ttgagctagc ggcaggcgga caatcagggg ctacgtgtta acgttctgac
      961 catgattgtc tatcctgcat tgctcttttg ccgcctcaaa atcctttgcg tgtttttgct
     1021 ccccgttctc cagaaaaaac cgagccgcca cggttccggc agcgccttga gcgctaccgg
     1081 aaaccgtctg gggagaaccc cagaccccca tcgctgcaac accgacggtc tacgaccacc
     1141 taccaaccgc atggctgcct gttccgtgca aatgggcttt gcagggggct ggcgcggttc
     1201 ctggcgacct gaaagcccct ggttaggggt atcggcgcgc ttgacctcct gagagcctct
     1261 gtaagcgttt ttcgcgcctt ccatgccctg gcgggcatct aaaacccgtt tcgtgcgtct
     1321 gagagctttt gagcgcgttt gaggggctat ctggcgactc atccccaaaa gaacaacacc
     1381 gggatcaccg acaacatgcg agaggtgatc ccgctcgccg caagtcgatg accgagcgta
     1441 gcgagcgaat cgacgaggaa gcggaagaga accggaagcc acattgagca cttacgcact
     1501 gatgcggggt gtcggggtga agccctgacc aagtggtaat cgtatcggc
//
