LOCUS       NZ_CP030334             7588 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_451 plasmid unnamed7.
ACCESSION   NZ_CP030334
VERSION     NZ_CP030334.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291544
            Assembly: GCF_003288455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 10.03x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003288455.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 02:15:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,980
            Genes (coding)                    :: 4,764
            CDSs (with protein)               :: 4,764
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 78 of 216
            Pseudo Genes (incomplete)         :: 153 of 216
            Pseudo Genes (internal stop)      :: 39 of 216
            Pseudo Genes (multiple problems)  :: 46 of 216
            Pseudo Genes (short protein)      :: 1 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030334.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_451"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
     gene            1633..1842
                     /locus_tag="CSC38_RS00025"
                     /old_locus_tag="CSC38_0001"
     CDS             1633..1842
                     /locus_tag="CSC38_RS00025"
                     /old_locus_tag="CSC38_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(1908..2441)
                     /locus_tag="CSC38_RS00030"
                     /old_locus_tag="CSC38_0002"
     CDS             complement(1908..2441)
                     /locus_tag="CSC38_RS00030"
                     /old_locus_tag="CSC38_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2444..3370)
                     /locus_tag="CSC38_RS00035"
                     /old_locus_tag="CSC38_0003"
     CDS             complement(2444..3370)
                     /locus_tag="CSC38_RS00035"
                     /old_locus_tag="CSC38_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3547..3945
                     /locus_tag="CSC38_RS00040"
     CDS             3547..3945
                     /locus_tag="CSC38_RS00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4149..5063)
                     /locus_tag="CSC38_RS00045"
                     /old_locus_tag="CSC38_0006"
     CDS             complement(4149..5063)
                     /locus_tag="CSC38_RS00045"
                     /old_locus_tag="CSC38_0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(6583..6918)
                     /locus_tag="CSC38_RS00065"
                     /old_locus_tag="CSC38_0008"
     CDS             complement(6583..6918)
                     /locus_tag="CSC38_RS00065"
                     /old_locus_tag="CSC38_0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_001080732.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMVL
                     YPVAKWYIEDTALKFTRPDFWNSGFFTDTPGKMGLLAVYTGTVFILSLPLSMIYILSVI
                     IKRLSVR"
                     /name="colicin E1 family microcin immunity protein"
     gene            7047..7394
                     /locus_tag="CSC38_RS00075"
                     /old_locus_tag="CSC38_0010"
     CDS             7047..7394
                     /locus_tag="CSC38_RS00075"
                     /old_locus_tag="CSC38_0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="WP_000142452.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPFM
                     PFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWWRR
                     KVNRLPPWDDV"
                     /name="DUF6404 family protein"
     Typing          complement(4289..5063)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(2448..3370)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga tccatttacc
       61 ataactcccg ttgtacgccc cccttaaaac gccctcagtc gctttctggc gcaaaaaacg
      121 caaaaagaca agaaaacacc aggccaaacc agaaaaacgc gtcagaatcg ttttcagggc
      181 gttgcataac tatgcacgaa agcgatgtgc gatcggtttc gtgcgttctg aacaccgaac
      241 ggctcaaaac gacaagtttt ctcccctggt gcgtggcgct ctgacggggt ttcctgtttc
      301 caaacttcac atctcgccag gacaaaaaaa aaccgacctg gggtcggtgg caagaatcag
      361 agtctatggg aaatatttta gttatcggat gctcaatatt cacaaacgca aaaaatgcct
      421 cagtgatgcc ctaaaacaac ctttccagaa ggagtccgcg ctttacgatg cacggaagag
      481 ataaaaaacc ccacaaaagt ggggaaccaa gagaaacaac aattacaggt tgttggttgt
      541 gttcgaacaa cgatgaagta gctacgctaa ataataatta ccgtacaggc cctacatatt
      601 caacactaaa tttaagatcc tgacgaagga tttataaatg cacgaaaaca catagacaga
      661 caaattcact aaattcatac acaaaaacaa gtgtagtttt ctaagtaaat tagattcgac
      721 aaccaattag tttatccaca tcgcattcca ctcttgctac accaatcaca tttcttgata
      781 tgtaatagca gaaaaaataa aaccagacac caatagacca aaaaacaaac attcaacaag
      841 aaacattttg aacaatatca caaaactgtc acaaccatca tcagactatt gaaaaaaact
      901 ggttgctgtt ttataaaagc gactgtaaca acctccactc tatagttcca ttagtttaaa
      961 gtgtcacaca aactgagcat ccatttgttt tccttacttt tgcccctgta ttccaagggg
     1021 tttttttata acttaaagat tagcacacca cacaaaaaca ataaaaaaaa acgacaagtt
     1081 atacaataaa accagtacta tccatacagc taattacata taaatactat taaaccatct
     1141 ggacattaaa aataaactct tataacataa aattaatgcg gttccggtac catatgtcag
     1201 ggataatgtg caaatggagg tatcggaact gcatttcagt tcattattaa ttaatgacaa
     1261 aaaaaacctc tgcgagcgca gagggggtaa ggaatgttcc cggtcataat atgtcgatag
     1321 tgtttatata catacagaac aagaacaaca accagccaac taataaatat tttatttttt
     1381 agagctgaaa ccttaaagct cacttccatt ccatacattg ttataaacct tatgttgaaa
     1441 actgaaagta agtacggcag acttattaag ttgcgtgcag tcttttcacg ttatacaaac
     1501 aagaacacct gcgaagttat ttctccttac atatccccct atgccaacgg gggatttttt
     1561 ctggttcact aacgcataaa taatagtgac accctatcat tcacaaaccg tgataatata
     1621 aggattatca tcatggcaaa attaggtgaa aacaccccac atattatcga tgctactgta
     1681 gattttatgg catcaagcca ggcatttcgt gagtatctga aaaaacttcc ccctgataac
     1741 acaataccgc cagctcttac tgatgagcac gtaccgttat atatacgaag gctggaatac
     1801 tatcgtctgc tttatcgacc aaagcagaca gaatcatatt gaaaaaaacg gtgaacggtt
     1861 cgcgaaattc tttcgcgggt cgtgaggcgt tggacaagcg aagcgcgtca gtttttccgg
     1921 ctcttccccg tgacaggttt ctccaccagt ttatcttcca gtgtcacaac tttctgatac
     1981 accgccgctg tggcgttgta gttcctgtcg agtattgttt tcattcccca tccgagcaca
     2041 cagaacataa ccagaaaagc cactgacacc ccacccagcc atttccagct atacccttta
     2101 agggtgttgt attcctgcac gatattgctg tgaattgtcg caatatccct gttcagggca
     2161 tccgcgtttg ccttcatgtt tgcgactgac tcttccagcc gctgaaaatc ctggctgatg
     2221 cgctcaccta tcagtcctgc cacgtccagc ttctgcacct catttctgac ttcatccctg
     2281 acctgttttg ccacaccgtc agaaatatcc cgatatccgc gaagagtttc ccttgccaca
     2341 gccagagaat cagcaacagc tttctgttgc tccattgcgg tatgttgcag atgctccacc
     2401 gataacagga gcgtaagtaa tttttcttct gtgctggcca tgttcagaat cccatagaaa
     2461 atccacgtga cggtgattta acccgttcag gtgcagttcg gttctgtctg ctcactctgt
     2521 ctctcatgtc aaagccatgc tcccgcgcaa catcatgttg cagccttctg accagttcat
     2581 gttgcttaac cggagtgtcc agccattccc tgatgtcatg cccgaccgtt cgcgcctgtt
     2641 caagatggcg ggaatagtcc ggttcagacc gtttttgcat gatgtcatga cgctgggcat
     2701 catcaagccg tccggcagct tcaaaaccca gatgcacttc gggacgtcgt tcaatgccct
     2761 gttctttcag cgtccggcag tcaatgaagc ccctttcaaa gccatgcttt ctcatatgtt
     2821 cgttgtgctt cacctcccag cgttttcgca attcccgcaa ctgctctttt tgctgagtga
     2881 gagtcaggct tccggaatcc tttttcgcac ctccccgctc gggatacctg gcgttatagc
     2941 gccggaaata ctgttccggt gtacgttcaa tgccatcatt cacacgctga ctcatcatga
     3001 tatgcgcatg aggctgttcg ccgccgtcaa tgctcgcttt cggattgtga atggcaaaac
     3061 tccaggcgtg ccgttcgcca gcttcctgac gtacaaaatc ctgcaccagt tcaaggcgct
     3121 gttccggtgt cagttcacgg ggtaatgcaa tttcaagttc cctataggtc gaaccgttcg
     3181 cacgttcaaa ctggtcagcc gcctgccaga aatgggaagg attatccctg gcccattccg
     3241 gcatgtttcc gtgtgcggaa tattccaaat cctgacggtc gcggtatttc tctttgcgct
     3301 cgatgtaatc ggcgtggttt gcagcctgcc ccttgccacc aaatttaacg ctgaggtgat
     3361 aggttgccac ggtttcagcc ctctgcagga gcgcactgtt tctcgacgaa ggagagaaac
     3421 atataagtgc gcttccttct aattttacga attagcacgg aagatacccc ctgcacccgc
     3481 cgaccgcaag cgatatactg cacgaccaca acacaccgca aaaaaacacc agggagcatc
     3541 acgaacatgc gcatcagcaa catcgaatgg ctgaagaaac ggatcggatt catcaggaag
     3601 ctcggagaac agaccgcacg acagagacag attattgacc tgatcgacaa cgaagccgga
     3661 ctcacagaac aggaacgcaa acttctccac gtactggcaa cagcggaaaa aaacgactta
     3721 caggcacagg agagcgaacg aaaacaggcg gtccaaaaac gaatcgaggg aaaaaaacaa
     3781 cgacgggaac gcaatcaccg gctctttctc gcggctgggc tactgatcga agcaggactg
     3841 gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca agcacttaag
     3901 gaacttaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc tccatccgtg
     3961 ttccgattcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat gagactgcgg
     4021 cgagcactac caccacaatc acgcggaaag ccgcgccatt gcagggctga acggggaaaa
     4081 acggcaaaaa acctgctgct caaaaagacg ccagaaaggc gctgttatct gatataggct
     4141 tatttggtct actctacgag acctcgtttt ttcagttggt aataccaggc gcgactgatc
     4201 cctaatgcct cccacggttt cagcgtgcgt gcagatgtag ctactgtgct gcgcttagac
     4261 ttagaaccgc ccttgcgacc gcgtgcagcc tgaatttctg gcgtgtgcgt atcagccaca
     4321 tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt atttcgcaat gctcttgcca
     4381 atagcccgac attcagccag tgaaagcgga acgggaagcg atgcattgta catttcgaca
     4441 cgctggatca ccgcttcaag ccactgtgag aacgctggcc agccctgacg aatcgccctg
     4501 taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc gccccatccc gtaatgttta
     4561 tcgatgctac gacgctctga ggcgctcaaa tcgagataat cagccagttc atcgagggta
     4621 taggcttcct cgcgccactc catcaccagc cattccaggt ggaacggatt tttgcaaatc
     4681 aggccgctgt agttcacatc cgcacaaagt ttctcacaca gcgaacgttc aaccgctgcg
     4741 gcatatttca gcgccttaac cgaggcatca ggcgcggttc tcacggcgat gttgagtgcg
     4801 tagagcaggt gagcgtgtcc gttcacagga tttttaacgg tgatgttcgg cgcgggagcg
     4861 ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa cgtcaaaaac cagccagaac
     4921 tgcgcatgag gctggttctg ctgtatgtac cgcgcaagaa gcgcacgccc tttgccggag
     4981 atgcgaagac caaaagcgag atcatccgtg tgatagggac gatggggtaa attttcgtcg
     5041 aagtattgaa gcgcggcgct catgtgttac gattctctcc gggttatgcg acctacacgc
     5101 cctctgtatt tcatgttgaa tcttgccgga tctggacatg atttcagggg cgtttgtttt
     5161 tcagtcgctt acactacacc caacggcaaa tcctgtcgaa ccttcccgaa aacccgatcc
     5221 atttaccata actcccgttg tacgcccccc ttaaaacgcc ctcagtcgct ttctggcgca
     5281 aaaaacgcaa aaagacaaga aaacaccagg ctaaaccaga aaaacgcgcc agaatcgttt
     5341 ttagatggct gcataactat gcacgaaagc gacccgcgat cggttttgtg tgttctgaat
     5401 accgaacggg tcaaaatgac acgttttctc tccctcaatg gagcgttcta tacagagatt
     5461 tttgttcccc tatccattat tctgattacc gggtttccaa gctgaaaaca ggacttttct
     5521 gcgaccagtt tttgcgaata tagtcacata aaccaccgat tgagtgacaa atacaggaga
     5581 atttattttg aaatatgatt ttatcaatgg cagttctatt ttaattatag tctggatgac
     5641 tatagctgta ataatcagag tcacaaactg gaaattcaac aaaaaaacag ccccagctat
     5701 aagcttattc ttagctttcc ctgcatctat agttattctg ttagtttata tttttttctt
     5761 cgataagtga tgtgttttca tgattaatca agtttctgtt caaatgctcc tgctggccca
     5821 gcatggaaaa agaaattacg aataaatgca cccagtgcca ttaacatcta tctacacaaa
     5881 aatagaggaa aacaattttg atatatcaaa taattacaag cagctccatt ctttttattg
     5941 tttgggggat tacatctata ataattagaa caaagaatta tttttctaag aagaaaacag
     6001 accttaatat aagctttttt atagccttcc ctatttctat aacaatcctt ttattttgcc
     6061 gaggtatatt ataaatataa cataatacat ctatatgggg tgtacatatc cccacataaa
     6121 aacacatact ctcattaaca cgggaagaaa ctatgttttc caattctcac tacatgtaca
     6181 taatcattct tgtactatgg tttagtattc tcaagtactt acagagaaag aatagcatat
     6241 tacatccttt attttcacta atcctgacac cactaaccat tttaatcatt agacttatga
     6301 tagatttaac atacaaccaa taaattataa acaaaataaa aatagggaac attatatgct
     6361 ttataaaatt ttcacattaa acacaaagat aaaaacaact atcattacaa tatgtgcatt
     6421 atcatataat cttacagcgc aagcttcccc gtcatacaga cctcgtgatc gagttaataa
     6481 gactatgata gaactacaaa ctaacggagt agaacggcaa cttttagata attcttgtgg
     6541 cataagttct ggggtattta aataaaatat aagacaggct gtctatctta cagacagcct
     6601 ttttataata acagaaagaa tatatatcat acttaacgga agagataata tgaaaacagt
     6661 acccgtataa acagcaagca atcccatttt tccaggtgta tcagtaaaaa aaccgctgtt
     6721 ccagaaatca ggccgggtaa atttcagagc tgtatcttca atataccatt ttgcaaccgg
     6781 atacaaaacc attccgcaga gagaaataca ccagaacagt aatctgtatt taaaatcata
     6841 atcccatgac atatacagca tatatccccc cgtcacccat ccccaccaca tattattaaa
     6901 ataatatttt ctgttcatct gccagttttc ctttcttttg cacttttgtc agtgtactga
     6961 tgcatgacaa cataaccaca gcatgataat aataatcaat aacaataagc tgtgtcacgt
     7021 ttacatcatg gaacggaggt gctcctgtga cttttgagca gaaaaaagcc cgggccattg
     7081 ccctgatgga cagcaaaaag atgtggcgga gcaattatgc ccctcctctt ctgcgtattc
     7141 tctggcggct gggtatccgg cttccgccat taccctttat gccgttctgg caggtcacgg
     7201 tgctgacggg gggcttatgg ggcatttcct ggggatgtgc aatgtggttt atctactggg
     7261 ggccgtcagg aatggtggca ggtgaggcca ttattataag tatcacgggg gggtttttgt
     7321 ccggactgct catggcctct ttccactggt ggcgcaggaa agttaaccgg ctgccaccat
     7381 gggatgatgt gtaaaccgtg cacaggcgtg ttcttacgga gcggcacttt catttatctg
     7441 tacgcgaacc tccattcctg catcgtgaca ggcctgcagc cactgcgcca cttccagcgg
     7501 atcgccctcc cggcgtacca ccctgccttc tttattccat aactgcagac aggtgctgcc
     7561 gtcgagacgc accacaaaat ccccacgg
//
