LOCUS       NZ_CP030333             4082 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_451 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP030333
VERSION     NZ_CP030333.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291544
            Assembly: GCF_003288455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 10.03x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003288455.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 02:15:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,980
            Genes (coding)                    :: 4,764
            CDSs (with protein)               :: 4,764
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 78 of 216
            Pseudo Genes (incomplete)         :: 153 of 216
            Pseudo Genes (internal stop)      :: 39 of 216
            Pseudo Genes (multiple problems)  :: 46 of 216
            Pseudo Genes (short protein)      :: 1 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030333.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_451"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
     gene            complement(251..952)
                     /locus_tag="CSC38_RS00005"
                     /old_locus_tag="CSC38_0012"
     CDS             complement(251..952)
                     /locus_tag="CSC38_RS00005"
                     /old_locus_tag="CSC38_0012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1485..1739)
                     /gene="mobC"
                     /locus_tag="CSC38_RS00015"
                     /old_locus_tag="CSC38_0013"
     CDS             complement(1485..1739)
                     /gene="mobC"
                     /locus_tag="CSC38_RS00015"
                     /old_locus_tag="CSC38_0013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1918..4077
                     /gene="mobQ"
                     /locus_tag="CSC38_RS00020"
                     /old_locus_tag="CSC38_0014"
     CDS             1918..4077
                     /gene="mobQ"
                     /locus_tag="CSC38_RS00020"
                     /old_locus_tag="CSC38_0014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1741..1917)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1918..2276
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(252..952)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1832..4074
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacaatattt tgttttgttt aacatcgtgt tatgttcgtc gatgttcttc gatgttcatc
       61 gacagggatg aatgcaagaa gcacacacat aatgcaagca tagttttgtt tttccgcccc
      121 caaaaaatgg gggttctttt ttcgctgcat tagagcgctt ttgacgcaat ttttcacttc
      181 aatagtacct tcgcactact aacacaaaaa aaacgcggca gggagcgttt taggtgcctt
      241 aaaatcgatt ttagatcgtc atcttgattt gtttgtcttc cttgaagcga aaatgcaggg
      301 ctacaaccgt gcgccccttt ttgatcgtct caaccgttac agccaggtcg ctttttttgt
      361 tcagttcgtc aatgcagggt cttaataatt gctggttaag tgatttatat gtttgatatt
      421 tatgctctat atcaagcatt gagcgaaaat catcgagagc gataatacgt tcgccagtac
      481 tgcggaactg ctggaggagt tcgtatatcc ttatactgta acttcttgat aatctagata
      541 tattcttgat tacaactcta gtaaattgcc cttgtagttg tgtcaaataa ggcattactg
      601 cgtcagaaaa tgttatttgt gctctggcct cgcctttagc gtactgtgcc cgatactgga
      661 tccagcggaa ctcctcacgt ttctcgtcat ccttgagaat gattgacctg tcccacaaac
      721 gatcaattgc ttcctgtaac tgaacctcgg catttttccg ccccatgtca gggaatgaat
      781 cgaaatattc cgcagctgta atggtcattg tcttttgcaa ttttggagat tctgcatcag
      841 ccccagactt caatcgaccg atgcaaagta acaaaaaacg ctgttcctgt agggtcaatt
      901 tataactggc ttcaaccagc gcattggatt tataggcaat tagatccgac atagagcatc
      961 cctccccaaa aggtaatgaa taaaagataa acataccctc aacgaaaggc aatgcctttt
     1021 tattttaata tccctttttc tgtgaatatt accttaaatc atattgcctt taccttaaat
     1081 tcctccccct aaacacctta aatcatatta cctttacctt aaatcatatt acctatagcg
     1141 ttgtaacact ttgttttcta taaggaattt tggcgctaaa acaagtatta aaaacaagat
     1201 cttaaaaaca agatctaaaa acttaacgac gagcctgtgg ataacttgga gtgctgaaaa
     1261 caaaaaacca ataaaaacaa tgcgttgcaa aaaagcagaa ttgcaagttc cgaatacccc
     1321 ccctccaacc ctgtggaaat ccagtattca tgcgggttca gcgcatatca cctcccgaaa
     1381 aaccgtgcgt atgtgctcaa tcaggcgacg gattcacttc cgcttcctcg cccactcgct
     1441 cgctaacgct cggtcgctgg gactgcggcg agcggacgat acaatcactg tcctggcagc
     1501 gcttccagct cgttaatcag cggtagtatg cgcgtcacat cagcctgtcg tgtcacctct
     1561 gccttcagga actggagtaa ccaggcacgc aactgcacct cctccggctt tctgaccttt
     1621 gccagcaatg ctgcacccac aatgaccttc tgcgctgtgt ccagactcct gctcttcgcc
     1681 ttcaggcgct gtaatctggc ctcagcatca gcaatcttct gttcgagtgt tctgctcatg
     1741 atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg tcactggtag tttaaaaact
     1801 gagctggcat aatgcacgga aaatcacgaa gtgcgcactt atacaatctc cactccgttt
     1861 cgattgtgtg cgccctgcgg ggctgaaaga aaaccacaaa aaggcattac ggcagaaatg
     1921 gcgatttatc atctcagcat gaaaatcatc tccagaaaca gcggctacag cgccgttgct
     1981 tctgctgcct atcgttccgg ttcactgatg cttgatgagc gtaccggact gactcacgac
     2041 tacacccgca aaagcggtgt ggctgaagct gtgattctga ctcccgcgac agcccctgcc
     2101 tggtgtacga accgtgccga actctggaat gccgttgaga aggccgaacg ccggaagaac
     2161 tcacaacttg cgcgggaaat tgaactcgcc atcccccgcg aactaccgca ggatgccgca
     2221 cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca gtcagggcat gattgctgat
     2281 gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct gaccacgaga
     2341 gctgtcggac ctgctggatt cggtggaaag gtcagggact ggaacgaccg gacacatgcc
     2401 gagacgtggc gtgcatcatg ggctgaccat gcgaacaggg cactggcgaa cgccggttat
     2461 caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gcctggtatt
     2521 cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa
     2581 cagaatcgtc tgattaacag acttaacctg gaaatacaga tttcccgtac cgaactcaga
     2641 aacagcgggc tgacagagcc tgcccgccgt gtggctgacc ttctgaatat cgtgcttcct
     2701 gacaacgcaa cttcgtacac actacgtgac atcatcgaag cgttgccgaa ggacagtaat
     2761 gcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca ggcgacgcgc
     2821 agacgctggg aagagctgaa caccgaacga aaggaagcga tgagccatgc tgccagcctg
     2881 aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ccctgatgca atgggcggca
     2941 ccggaatacc gtagcgaaca gggaaaaatc cagtcgctga gccagcaaat ggaaaaattg
     3001 cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagac ggcatttgaa
     3061 aaacagtggc agcagtgggg ggcatccggc cttgcagctc tcagagtcca gttgcttaaa
     3121 cgtgaagagg aattgcgccg gaaggagcag gaggatacag aacgtagtcg cgcggagcga
     3181 cgagcggaac agctcaggaa aaatgacaat gccgttatgg attcaggggt actgagcgca
     3241 gtcgttacgg ggtatggcga ggcccccatg cctggtaacg gggaaatgac ctgttacctt
     3301 ctgcttcaca accggaacgg agaatacacg ctctggggga acgagctgga gaaataccgc
     3361 accagaatct ttgagcgtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga
     3421 agcgaaatcg gtcaacatcc accactgcaa cgtgtttatt cctctgcgac ctttgagcaa
     3481 cttctgacgc aggtttgcca gacatggccg caatataccc gcaatctccg ccagccgaag
     3541 acatggccgg agtctttctg tcttggagaa gaccgccagc cagccatgcc atcactggca
     3601 gcaaggaagg tggattttac gcagggacgt ctgctaccga cgctgatgcc ggtaatgagt
     3661 tctgtcgacc gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct
     3721 ctgggcggtg ttgtccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc
     3781 gataacagcc agctggtcat cagtgcactg accgataagg ggttacggta tgcagggtat
     3841 ggtgttgccg taaatcacga tgcagacagc cacatatcac ctgcgccgga acttatggag
     3901 tttcatctga aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag
     3961 ccggatcatc tgttccgctc gctgggtttt aatcgtacct gggacgaatg gcggcgcgag
     4021 gaagatgcac gaacacacgc caccgagagg agacatgatc gcggttggtc acagtgaatg
     4081 tg
//
