LOCUS       NZ_CP030332             4593 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_452 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP030332
VERSION     NZ_CP030332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291545
            Assembly: GCF_003288415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 19.79x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003288415.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:54:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,824
            CDSs (total)                      :: 4,706
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 74 of 171
            Pseudo Genes (incomplete)         :: 102 of 171
            Pseudo Genes (internal stop)      :: 46 of 171
            Pseudo Genes (multiple problems)  :: 44 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_452"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
     gene            complement(554..1393)
                     /locus_tag="CSC39_RS25670"
                     /old_locus_tag="CSC39_0005"
     CDS             complement(554..1393)
                     /locus_tag="CSC39_RS25670"
                     /old_locus_tag="CSC39_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(1585..1734)
                     /locus_tag="CSC39_RS26700"
     CDS             complement(1585..1734)
                     /locus_tag="CSC39_RS26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            complement(2404..2595)
                     /locus_tag="CSC39_RS25685"
                     /old_locus_tag="CSC39_0006"
     CDS             complement(2404..2595)
                     /locus_tag="CSC39_RS25685"
                     /old_locus_tag="CSC39_0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2658..2981
                     /locus_tag="CSC39_RS25690"
     CDS             2658..2981
                     /locus_tag="CSC39_RS25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3190..>3624
                     /locus_tag="CSC39_RS26495"
                     /pseudo=""
     CDS             3190..>3624
                     /locus_tag="CSC39_RS26495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3659..4219
                     /locus_tag="CSC39_RS25965"
     CDS             3659..4219
                     /locus_tag="CSC39_RS25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            4226..4459
                     /locus_tag="CSC39_RS25970"
     CDS             4226..4459
                     /locus_tag="CSC39_RS25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2232..2316
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(1663..2221)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2972..4557
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcacgcccta cccggatggc gccaatgtta atgtacgtac attagcatta acaatatcca
       61 aacctcccca tttttccccg ttcgacggct ggaacgtgcg aaactgatcg tcattcagtt
      121 tcgcgcataa ctatgcatga ggttaaaatt taccaggcgc gatcgcggca gtttttcggg
      181 tggtttgttg ctgtttttac ctgtctgctg ccgtgatcgc gctgaacgcg tttaagcggt
      241 acgcgcaatg cgacgttatg gtaaatacgg agtattttaa ttgcgggcct ttgttgtata
      301 tctctgtcgt ttgtccccgg ggggaagcat tactgctaac aagtcccgtg agaaaactgc
      361 gcaatcgtat tgcgcaccat gaacctattc tcaaccgcaa tctggaggat gattttgcta
      421 cgataaagag aattatcgcg tatcggtgtc agcattcatt ggagtggatg ctgaaaaacc
      481 aggtgttact ccctctactt acacttaaac ctctctaaaa atataagtaa gtactatcga
      541 gggctatcat gaactatggt gccctctgag catagctacc cattacttct tctatgtcat
      601 tgaataattc atttcttaac tctaagtatt ctagtaactc catttctaat gactctgtcg
      661 tgaacttaaa tctagaagga tctttgattg agtttactag tcttgataac gctctatatg
      721 ttttgagtga aggttgcatt atagatggaa agtatttgaa aacctcttca tctctgttga
      781 aattaactaa aaacttgtag tcatcattta taaatctcaa gcatacagcg tcaagcttat
      841 cgccttttct tattattatt tctttatagg aaagatattt ttcagaagat aagtcccaca
      901 tcaagcgaaa atctctcttt gcgaacgagt tcgcgtcatc attgaaaaga gatgatgtga
      961 tggctttgga tagaccttct gtgtttatct ctttgttgga tgggtagtaa attttattaa
     1021 ttgctttcat tctctcaaat gatgaatata ggtcattatg ccaccttgtt gaaatcactc
     1081 ctttagttat attctccttg ctaaaattaa gccctgtatc aaaaataaat gcagccatca
     1141 gtgaatatga tatgtttttt attaactcag acttaaatgg ttccgctatg gagtagtaca
     1201 gcgataaaat aactaaaaat aaaaggtatt gtcttacctt tcccgatgag tatatgtaaa
     1261 ggcgatattt atttattgat agttttattt tgtatggaat gatctcattt ctgtcatatc
     1321 ctcttcgtgg tggagcaaaa caaaagaaac atgcagctat taataaaaca aaaaagatga
     1381 acagtgtaag caagttcggc tcctgtgtat gctcactatg catgattgtc ttgagatcta
     1441 acgatcctga aggtagtctt gtccgtaaac gaaaaaaccg cccagcaagg cggcttttcg
     1501 aaggttctca gagcagcaac tctttgaacc aaggtaactg gcttggagga gcacagccac
     1561 caaatctgtc ctttcagttt agccttaacc ggcgcataac ttcaagacta actcctctaa
     1621 acggttacca atggctgctg ccagtggcgt ttttacgtgt ctttccgggt tggactcaag
     1681 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1741 cagcttggag cgaacgacct acaccgggcc gagataccaa cagcgtgagc tatgagaaag
     1801 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1861 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1921 gtttcgccac ctctgacttg agcatctgtt tttgtgatgc tcgtcagggg ggcggagcct
     1981 atggaaaaac gccagcggcg cggccttctt cctgttcctg gccttttgct cacatgtttc
     2041 ttttggcgtt atcccctgat tctgtggata accgcatcac cgtctttgag tgagctgata
     2101 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
     2161 gcctgatgcg gcattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     2221 cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc cgctatcgct
     2281 acgtgactgg gtcagggctg cgccccgaca cccgctaaaa cccgcagacg cgccctgacg
     2341 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg aacgtctccg ggagctgcat
     2401 gtttcagttg ctttcaccgt catcgccgaa gcgtgcgagg caactacggt agagttcgtc
     2461 ggcgtggtcg tgaagtgatt cgcagatgtc tgcctgttcg tccgcgtcca gctcgttgag
     2521 tttttccagc agggtcagcg tctggcttct gataaagcgg gccatgttaa gggctgtttt
     2581 ttcctgtttg gtcatggtct taaggctcct taatagggga ttttttaggg ggaatgatac
     2641 cgatgaaacg cgagaagatg ctcacgatac gggttagcga tgaggagcac gcccggttac
     2701 tggaacgctg cgagggtaaa cgactggcag aatggatgcg gcgggtctgt ctgggggagc
     2761 cggttgcgcg cacaggaaaa ctgccgacac tatcgccgcc attgctgcgg catctggctg
     2821 ccatcgggaa caatctgaac cagacagccc ggaaggtgaa cagcggccac tggtcttcga
     2881 ttgaccgggt tcatgtggtg gccgctttga tggccatcga gggtgaattg cgccagttgc
     2941 ggcaggctgt cagggaacag gggggacggg atgatagtta aatttcatgc cagggggaaa
     3001 ggcggtggaa gtggcccggt cgattacctg ctggggcggg agcgtaaccg cgaaggcgca
     3061 acggtgctcc ggggtaaccc ggaagaaatc cgggaactca ttgatgccac gccattttcg
     3121 aagaaataca cgtcaggcgt tctgtcgttc gcggagaagg agttaccgcc gggtgaacgc
     3181 gaaagagtga tgacgagctt tgagcgtgtt cttatgcccg gtctcgaaaa agaccagtac
     3241 agcattctct gggtggaaca ccaggataag gggcggctcg aactgaattt tgtcattccg
     3301 aacatggaac tacagagcgg taagcgcctc cagccgtact acgaccgtgc ggacaggccg
     3361 aggataaatg cctggcagac gctggttaat catcattacg ggctgcgtga cccgaacgca
     3421 ccggagaaca gacggacgct tgtcacaccg aataatctgc caaaagcgaa acaggaggcc
     3481 gcagaagcga ttacacgggg tcttgaggcg ctttaccgtg ccggagcaat aaaaacgcgt
     3541 caggatgtca ctgaggcgct cacagcggca ggttttgagg tggtgaggac aaccagaagc
     3601 agtatcagta ttgccgaccc ggaggggggg cggaatctca ggctaaaggg ggcgctgtat
     3661 gagcaatctt ttacaaatgg gaacggactt cgagaagaaa ctgaaagagc gagccgcttc
     3721 taccgagaac atgctgaaca gcgagttcag gaagctagaa gagtctgtag acaaggcact
     3781 gagcttaaac aggcagaaaa tcagagacgc tatcagcgaa catacaacat ccgtgaaaca
     3841 gcaactggat acgctgagca ccaccgtgag cacgcagctc agcaccacag aggccgaact
     3901 gtcccggcag cagaaaaacc tgctctggca ggtgatcaaa gggcgggtac tgtttccggc
     3961 gctgaccgcg ctcagcgtca cgggcggcat tttcctgggg tgctgggggc tgattcagtg
     4021 gcaggaaagc aggattgcga aaaatatctt gaccatcaga gagcaggaaa acacgctggc
     4081 gaaactcgag gcaaaaacct ggggggtgac gttcgtcaat ggcgagaacg ggaaatttct
     4141 ggtgctgccg gacggggtga aaggggaaaa cacctggacg gtgggcgaca agaacgccgt
     4201 gaggctggtc agggagtaaa cgagtatgac ggaactggaa gggcatttgc tgaacgcctt
     4261 agagcatttg caacaggact atatgcggcg gctgaacgag tgggagagcg ccttcgcaga
     4321 attgcagaag atgcacgcgg ttacgcaaca gaacaacgcg atactgaacg agcgtgtcgt
     4381 gaacttgagt cagcaggtgc agctattagc cggacaagtc gaccgcttga gtcggttatt
     4441 tatcacgaac aacagataag ggatgaacag gagcgtcagg cgcaggagct tgcccggcgc
     4501 tggcatgaag agcaggaacc cgaagcgcgg atctggcgtg gcccgtcaat ggggatgtga
     4561 ccggggtacc ccctgaaatc gagccagttt gtg
//
