LOCUS       NZ_CP030130            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 160111 plasmid p10K_L111, complete
            sequence.
ACCESSION   NZ_CP030130
VERSION     NZ_CP030130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09279554
            Assembly: GCF_003429545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Lai,Y.-C., Tang,H.-L., Chiou,C.-S. and Lu,M.-C.
  TITLE     Genomic analysis of Klebsiella pneumoniae sequence type 11 carrying
            both blaKPC-2 and blaOXA-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Lai,Y.-C., Tang,H.-L., Chiou,C.-S. and Lu,M.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2018) Microbiology and Immunology, Chung Shan
            Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung 414,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 10.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:17:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,850
            CDSs (total)                      :: 5,729
            Genes (coding)                    :: 5,550
            CDSs (with protein)               :: 5,550
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 83 of 179
            Pseudo Genes (incomplete)         :: 94 of 179
            Pseudo Genes (internal stop)      :: 14 of 179
            Pseudo Genes (multiple problems)  :: 12 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030130.1.
            Bacteria and source DNA available from Yi-Chyi Lai,
            No.110,Sec.1,Jianguo N.Rd.,Taichung City 40201,Taiwan.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="160111"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p10K_L111"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.08 N 120.37 E"
                     /collection_date="2014-12-02"
                     /collected_by="Chung Shan Medical University Hospital"
     gene            complement(join(9916..10060,1..113))
                     /locus_tag="DL890_RS27580"
                     /old_locus_tag="DL890_27610"
     CDS             complement(join(9916..10060,1..113))
                     /locus_tag="DL890_RS27580"
                     /old_locus_tag="DL890_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(123..1808)
                     /locus_tag="DL890_RS27585"
                     /old_locus_tag="DL890_27615"
     CDS             complement(123..1808)
                     /locus_tag="DL890_RS27585"
                     /old_locus_tag="DL890_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2135..2380
                     /locus_tag="DL890_RS27590"
                     /old_locus_tag="DL890_27620"
     CDS             2135..2380
                     /locus_tag="DL890_RS27590"
                     /old_locus_tag="DL890_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2654..3025
                     /gene="tolA"
                     /locus_tag="DL890_RS27595"
                     /old_locus_tag="DL890_27625"
     CDS             2654..3025
                     /gene="tolA"
                     /locus_tag="DL890_RS27595"
                     /old_locus_tag="DL890_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3022..3387
                     /locus_tag="DL890_RS31975"
     CDS             3022..3387
                     /locus_tag="DL890_RS31975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3562..3840
                     /locus_tag="DL890_RS27605"
                     /old_locus_tag="DL890_27635"
     CDS             3562..3840
                     /locus_tag="DL890_RS27605"
                     /old_locus_tag="DL890_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            3828..4142
                     /locus_tag="DL890_RS27610"
                     /old_locus_tag="DL890_27640"
     CDS             3828..4142
                     /locus_tag="DL890_RS27610"
                     /old_locus_tag="DL890_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4306..4734
                     /locus_tag="DL890_RS27615"
                     /old_locus_tag="DL890_27645"
     CDS             4306..4734
                     /locus_tag="DL890_RS27615"
                     /old_locus_tag="DL890_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4760..4939
                     /locus_tag="DL890_RS27620"
                     /old_locus_tag="DL890_27650"
     CDS             4760..4939
                     /locus_tag="DL890_RS27620"
                     /old_locus_tag="DL890_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4966..5496)
                     /locus_tag="DL890_RS27625"
                     /old_locus_tag="DL890_27655"
     CDS             complement(4966..5496)
                     /locus_tag="DL890_RS27625"
                     /old_locus_tag="DL890_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5503..6234)
                     /gene="mobC"
                     /locus_tag="DL890_RS27630"
                     /old_locus_tag="DL890_27660"
     CDS             complement(5503..6234)
                     /gene="mobC"
                     /locus_tag="DL890_RS27630"
                     /old_locus_tag="DL890_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6234..8198)
                     /locus_tag="DL890_RS27635"
                     /old_locus_tag="DL890_27665"
     CDS             complement(6234..8198)
                     /locus_tag="DL890_RS27635"
                     /old_locus_tag="DL890_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9682..9831)
                     /locus_tag="DL890_RS27645"
                     /old_locus_tag="DL890_27675"
     CDS             complement(9682..9831)
                     /locus_tag="DL890_RS27645"
                     /old_locus_tag="DL890_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6808
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9001..9579
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5507..6234)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 attcccaagc tttcaatgac agaaccatcg tcaccaaagt ctttagaata ctcttcccct
       61 ttaaactctt cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc
      121 tctcaaagat attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt
      181 ttgggatcaa atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg
      241 tgctgggaat cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta
      301 ccaccaccac cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt
      361 atgtcttctg ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga
      421 ggcttgttct tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt
      481 ttccggctct ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt
      541 gcgtttaaag atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca
      601 agatagcgaa cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca
      661 aaatcatctt tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc
      721 cttgcaccgg ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt
      781 ttatcttcgg catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc
      841 ggatgagcgg cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc
      901 tgtgccgggg taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc
      961 gggaaacgaa taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa
     1021 tcaccttttt cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt
     1081 acgctcacct gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt
     1141 gtcggtttcg catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc
     1201 tgccgctcat ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc
     1261 agtggtaaag tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt
     1321 tttgacatca ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca
     1381 atcccccaca gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg
     1441 gccgacaacg catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg
     1501 gcaggcagac cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta
     1561 tgattgccgc tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc
     1621 ggattatagt tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg
     1681 tttggatccg taccaccacc tgatgaaggg ccacctttac cagacgtacc gttcacattc
     1741 ccactggcct gaccaccatt atgtcccaga cctgaattac ccggacctcg accgtctcca
     1801 ccactcatac cgttttcctc tttgacattt aaaacaataa attaaagcca acatactgta
     1861 catatacaca gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat
     1921 tagttctcat cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt
     1981 ttcccgaaaa tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat
     2041 ttcagatggt atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa
     2101 ctgttcggga aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc
     2161 tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg
     2221 gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat
     2281 tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac
     2341 ggaaaccacc ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc
     2401 gcattgcccg caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag
     2461 gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg
     2521 cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt
     2581 tatgtaaatg aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca
     2641 ggtcatcaac ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact
     2701 cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc
     2761 atctaaaatc agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga
     2821 accagaggcc tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac
     2881 tctgaacagc gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc
     2941 cgttacccac gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa
     3001 cgcccctctg gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc
     3061 ctcactccgg tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg
     3121 gtacaggccg cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac
     3181 gatggacgag tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg
     3241 acggcaatga ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat
     3301 tttgaattca gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag
     3361 ctcaatgatg atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct
     3421 ggatgatgag tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag
     3481 caaaagccgt atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca
     3541 tatgactaca aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt
     3601 aaggaccggc tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg
     3661 gaggcaatca ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga
     3721 caggccgcat ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa
     3781 gcctggataa gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa
     3841 cgatttccgc gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa
     3901 atcggctggc agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa
     3961 cattgccaga cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga
     4021 tcgggtttgg cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga
     4081 ttgtgatcgt caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct
     4141 aataaccgat gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc
     4201 gcgtcagaaa accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt
     4261 gagaaattac acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg
     4321 ctcaaccttt cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat
     4381 gtaaaacagg gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg
     4441 tcagaactga tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt
     4501 tcacaccgtg aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag
     4561 gaactggccg atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa
     4621 gaagaacgcc gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg
     4681 acaacagaac tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt
     4741 accgaaggga atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt
     4801 cattaaggat cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc
     4861 ggccgacatg tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga
     4921 taagctcggg gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa
     4981 cggccaccac cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata
     5041 gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc
     5101 gtgccgctcg ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc
     5161 atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc
     5221 acacagcttc attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa
     5281 gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat
     5341 cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag
     5401 caccaccatg tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc
     5461 ttccacgtcc tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa
     5521 tttaaagcac gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt
     5581 ctgtggattc acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg
     5641 ccggtcaggg cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata
     5701 cccggacacc acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc
     5761 caccgttctg ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat
     5821 acggtgcagg taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg
     5881 cacggccagc cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac
     5941 gtccgtcagc ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca
     6001 cacgctgacc tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac
     6061 cagactgtcc aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac
     6121 cagctccgcc gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc
     6181 cgcgttccgc gcctgccgct tcgcgtggct gacgttgtac tgctccagtg tcatcagtaa
     6241 agccccgcaa aatcatcatc cgcttttggt ccgccgccgt cggccggagg caggtactcc
     6301 gccagcgggt ccgttttcgc cagcaccggg aaggttttca gcgaaatttc ccggcgctca
     6361 acccgcagcg ggctgctgaa cgccagccgc gccacgccca gcccggtcat caccgcgaac
     6421 ccgtcaggca ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg
     6481 aagctgtcgc cgctgatgtg ctccacgttc cccgcctccc ggctggcccg gcggcgctcc
     6541 acgtccaccc ggatctcccc ggtctggccc gccgcccact gcgcggactc ctggctggcc
     6601 gcgcgataga accagcggat cggcgtgtta tccagtaccg tggttttcac aaaatcagcg
     6661 ggcagatcct gcccgcactg accgaagtcg cccagcgact ggtgcgcgag cagcaggttg
     6721 cagtcccggc tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag cagatattta
     6781 atctcatcga gcatgatgct cgcgtgcggc cacctccgga actcatcccg cgcaatgatg
     6841 atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc atcgtccatc
     6901 gagccgatga cgtacaggca gccgccgccg ttgattatcc ccgccacatc gataccgctg
     6961 tccgtctgca ggacgctcag gtcggccacg ttctccagct tggtgatcag ccccttcacg
     7021 tcctttttca gggcctccgg caacgcgtac gccgccgcca gcacctgctg aatgtttgcc
     7081 ccctgcggga actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg
     7141 tccgccacgt cgccggtctc actcaggtta aacccggcaa ccagcagatt cttcagggcg
     7201 tactggtcga gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc
     7261 agggtgaagt ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc ccctttgggg
     7321 tcgaagtaca ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc
     7381 atcgcgctct tgccgctccc gggcaggccg aggatctgaa tattggtctt gcgccaggta
     7441 tcacgaggga ccagcaccgg ctggccctgc tcatccttac ccagaaacac cgcctgctgt
     7501 ttccgggcct gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg ctccccgaaa
     7561 tgcaggtggc ggacgtctgt catttctccg gtccgctggc tgctgcgccg gctctcctga
     7621 ttcagtttcg cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag cagcaggaac
     7681 gccagcagcc gcgccagaag gacgccggcg acaagccctc cggcacacca ggctatcccg
     7741 aacaggttag ccggctgcag caggtaataa tctgcgttcg catagaggtc cgggcgcagg
     7801 acggtttctc cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga
     7861 ccggcagcgc cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc cctgaccatc
     7921 cgcccgggtg aatccagcga ggccagcacc gaaaatggca cagtccggtt attatcgcgc
     7981 ttatagcgat cggcatactc ccgccgcgcc ttatggctca gccccacaaa acgcattcgg
     8041 ccggcccaga gttcagccag accgaaaaac aggccgataa ccagaaaaaa accggccaga
     8101 ctgtaatact cccggtacag ccagctgacc cacgggtaaa tctgccgggc cagctccggg
     8161 atatgccact gccaggcaag cgaatccgta ttaaacatgc aatctctctc cttcagacga
     8221 cgggtttacc ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg ccccaaggca
     8281 tggcggcggc ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga ggccagcctc
     8341 tttccgcccc atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt
     8401 gagaggaaaa ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt
     8461 cgccatagcg agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgtcaggt
     8521 gcgtccagca ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga
     8581 atttctggcg tggccggcgt ccagccggac agagccagaa agaggggaaa agaccgtgcg
     8641 tcggtggcgt ccagccagcg taagcaaggt cttttcccct gaagtcagcc acgccccagc
     8701 ggggatgact tgcatttctc cgcaaggaga aaaaagcaac agagataggt taacgtaaaa
     8761 tgttcagcga aaaaccgaca tggttcacct atcctgataa ttgatcgtca gggaatagaa
     8821 agacttaatc aggggacaat atcccacatc agcgcgtccg gtgaccgtct tgcaggtgcc
     8881 ggggaaattg cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag actgcggcgg
     8941 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
     9001 tgaggcaaaa cggcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca
     9061 gagttcacat aaacagacgc tttttcggtg catctgtggg agccgtgagg ctcaaccatg
     9121 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggtaggt
     9181 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccgtt
     9241 ctcccttgcg ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg
     9301 taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc
     9361 gccttatccg gtaactgttc acttgagtcc aacccgggaa agcacgcaaa acgccactgg
     9421 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
     9481 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
     9541 cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
     9601 ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc
     9661 aagaagatcc tttgatcttt tctactgaac cgcgatcccc gtcagttcag aggaggacga
     9721 tggtgccacc gttccgccct gaacatcccg gatataattt gcctgacatg cggccagaag
     9781 aaagccagca acaatcaata taaaaagaaa aatagtcttt acctttttca tatactttcc
     9841 ctgtatttaa agactccggc attcatccct gaacaccgga agtcagtatt tcaaatcatc
     9901 cttgataatt ttttgttacc agttaccgtc ccgataatca aaagctatat aacaattatc
     9961 cacatcaggt tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc attctttctt
    10021 caaatcaaac tcaccattat taatattatc ctttaaaggc
//
