LOCUS       NZ_CP030108             2359 bp    DNA     circular CON 04-JAN-2024
DEFINITION  Acinetobacter baumannii strain DA33382 plasmid pDA33382-2, complete
            sequence.
ACCESSION   NZ_CP030108
VERSION     NZ_CP030108.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09460321
            Assembly: GCF_003296225.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     High prevalence of unstable heteroresistance caused by gene
            amplification in clinical bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2018) Department of Medical Biochemistry and
            Microbiology, Uppsala University, Husargatan 3, Uppsala 75442,
            Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics workbench v. 11.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 18:00:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,935
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,766
            CDSs (with protein)               :: 3,766
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 36 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030108.1.
            Bacteria and source DNA available from Dan I Andersson.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="DA33382"
                     /serotype="ST1567"
                     /isolation_source="Tracheal secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pDA33382-2"
                     /geo_loc_name="Germany"
     gene            53..253
                     /locus_tag="DPV67_RS19225"
                     /old_locus_tag="DPV67_19225"
     CDS             53..253
                     /locus_tag="DPV67_RS19225"
                     /old_locus_tag="DPV67_19225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064847544.1"
                     /translation="MNIWAQYQRGGGRWDVRSASCRPRDADMRADIYFQPENEKTERVS
                     TRELTQNDLKPSLSDYKLPLA"
                     /name="hypothetical protein"
     gene            790..1974
                     /locus_tag="DPV67_RS19235"
                     /old_locus_tag="DPV67_19235"
     CDS             790..1974
                     /locus_tag="DPV67_RS19235"
                     /old_locus_tag="DPV67_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115284.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_064847543.1"
                     /translation="MNIFYGENPSSASLKSLENSQSLGIKTKSHVMLTPQGFQRVHDYL
                     LQDQSRKLLPKERVSKCRRLRIDKTKTRTVMYNEHREKAHYGNVQICGSIWSCPVCAKQ
                     ITQKRRNELGKGIESWKTVHNGSVYLLTLTFSHSPDQSLKSNLEGLKRAMKRFYETTRV
                     QAIFKKLSVFHKIKGLEVTYGQNGWHPHHHVLLLAEHHDLRFKDYTSELTELWIKACIK
                     SGLNAPSMRHGLDLRNGSYADQYVSKWGLEDELSKGHVKKGRNGGFTPFDLLNFSIEDN
                     EIYGKKPSKLFQEFAISMKGARQLVWSRGLKKLLGIEEKSDEELAVETDKASITLNRVE
                     DLVFELLCRYQLRHQYLEAIKHDYETGSFGSGLADQLIEQVVNYEIKQMQQVFS"
                     /name="protein rep"
     gene            2040..2195
                     /locus_tag="DPV67_RS19240"
     CDS             2040..2195
                     /locus_tag="DPV67_RS19240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153565175.1"
                     /translation="MATEMRQKFNCDVIFNVAEAEDTTMRMDGDLTFDEMFEPQEIFSK
                     YMEPMH"
                     /name="hypothetical protein"
     Typing          785..1974
                     /name="rep_cluster_586"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.58"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacaacatg aaaacatgac aacaacttag gctatctatg caccactggt gcatgaatat
       61 ttgggcgcaa tatcagcgtg gcgggggtcg gtgggatgtg cggagcgcgt cctgccgccc
      121 gcgcgatgct gatatgagag cagatattta ttttcagcct gaaaatgaaa aaacagagcg
      181 ggtgtctacc cgcgaactga cacaaaacga tttaaaacct tccctctctg attacaagct
      241 ccctttagcc taagaacggg agttaccgag ctttgctcgc aggatgcttt tgtttctcga
      301 atgagaaacg cgtaagcgtg cattcctgaa aaaacaccct aatttgctag gtggaaaaca
      361 cacaaagaac ctgtactaaa actagggaga ataaatataa gaaaggagat tcacttatgc
      421 ttgattatga ttttattaat ttgattcttt tcgttttatc gaccttgtac acaattttgg
      481 ggtattacaa aaaatgatat tccaatctat taaaaacaag gcgggacaat tacgaggagt
      541 gaggaggagg gcacgcacga ccgacacgac cgaggaattg gcagagcctt gtatttactg
      601 cgtgtatctc tcgtatatac ggggtgttgt catgaaaact tgttgtcttg cttttaggaa
      661 tttttcagta gtaatatcaa cttgcactaa agtcctgtgc gaaagaccta gtaaaccgta
      721 caggcataaa aatgggctga cagtgctgga acacttcaac ccagtgatca acataattaa
      781 ggtatgtcga tgaatatatt ttatggggaa aatccgtcct cagcaagctt aaagagcctc
      841 gaaaatagcc agtcccttgg tataaagacg aaatctcatg taatgcttac gccacaaggc
      901 tttcagcgtg tgcatgatta tctattacaa gaccaatcta gaaaacttct tcctaaagaa
      961 cgtgtatcta aatgtagacg tctccggatc gataaaacta agactagaac tgttatgtac
     1021 aacgagcatc gagagaaggc tcattatggc aatgttcaaa tctgcggttc tatttggtca
     1081 tgtcctgttt gtgccaagca aatcacacag aaaagacgta atgaattagg taagggcata
     1141 gagtcgtgga aaacggttca taatggctct gtatatctcc ttacgcttac ttttagccat
     1201 tcacctgacc aatccctcaa aagtaattta gagggcctta aacgcgcaat gaagcgtttt
     1261 tatgagacaa ctcgagttca ggctattttt aaaaaactat ctgtttttca caaaataaaa
     1321 ggcctagaag ttacatacgg tcagaatggt tggcatcccc atcatcatgt acttctttta
     1381 gctgaacatc atgatttacg ttttaaagat tacacttctg aattaacgga gttatggatt
     1441 aaagcctgta ttaaatcagg attaaatgct ccatcgatgc gccacggttt agatcttcga
     1501 aatggctctt atgctgacca atatgtgtct aagtggggcc ttgaagatga actttcgaaa
     1561 gggcatgtga aaaaaggtcg caatggtggt tttacccctt ttgatctttt aaatttttct
     1621 attgaagata atgaaattta tggaaaaaaa ccttctaaac ttttccaaga atttgccatt
     1681 tctatgaagg gtgctcgcca actagtttgg tctcgaggtc ttaaaaaact tttaggtatt
     1741 gaagaaaaaa gtgacgagga acttgcagta gaaactgaca aagcttccat tactttgaat
     1801 cgtgttgaag atctcgtttt tgaactttta tgtcgttatc aattgcgtca tcaatatctt
     1861 gaagcaatta aacatgatta tgagactggc tcttttggtt ctggattagc agatcaactt
     1921 attgagcaag tagtgaatta tgaaattaaa caaatgcagc aggtattttc gtgattgaag
     1981 caatttatat ttccataggt aagattcatc gaaatgtacc taaactaatt tgggataaca
     2041 tggccacaga aatgcgccaa aaatttaatt gtgacgtgat cttcaatgtt gctgaagctg
     2101 aagatacgac tatgcgtatg gatggtgatt taacttttga tgaaatgttt gaacctcaag
     2161 aaatatttag taagtacatg gaaccaatgc attaattatt aaatcactta atggcgtttg
     2221 gcgtgcgatg cacgcacata acgccattaa gagagttctt attagcctgg aatattccag
     2281 gcttttttgc gtgtacgcat aaatcaaaaa gaaccaaatt gacaacatga caacatgaca
     2341 acatgacaac aagacaatt
//
