LOCUS       NZ_CP030009             4096 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Enterobacter hormaechei subsp. xiangfangensis strain Pb204 plasmid
            pPb204002, complete sequence.
ACCESSION   NZ_CP030009
VERSION     NZ_CP030009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09389154
            Assembly: GCF_003254805.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. xiangfangensis (Enterobacter
            xiangfangensis)
  ORGANISM  Enterobacter hormaechei subsp. xiangfangensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ho,N.R., Kondiah,K. and De Maayer,P.
  TITLE     Complete genome of Enterobacter xiangfangensis Pb204: a South
            African strain capable of synthesising gold nanoparticles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Ho,N.R., Kondiah,K. and De Maayer,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2018) School of Molecular and Cell Biology,
            University of the Witwatersrand, Private Bag 3, University of the
            Witwatersrand, Johannesburg 2050, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-DEC-2017
            Assembly Method                   :: SPAdes v. 3.9.0
            Assembly Name                     :: FINAL-Pb204N1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 389.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003254805.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 03:02:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,647
            Genes (coding)                    :: 4,575
            CDSs (with protein)               :: 4,575
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 49 of 72
            Pseudo Genes (internal stop)      :: 9 of 72
            Pseudo Genes (multiple problems)  :: 14 of 72
            Pseudo Genes (short protein)      :: 1 of 72
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030009.1.
            Bacteria and source DNA available from Kondiah Kulsum, Department
            of Biotechnology and Food Technology, University of Johannesburg,
            South Africa.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei subsp. xiangfangensis"
                     /mol_type="genomic DNA"
                     /strain="Pb204"
                     /isolation_source="acid mine decant and tailings from
                     uranium mine"
                     /sub_species="xiangfangensis"
                     /db_xref="taxon:1296536"
                     /plasmid="pPb204002"
                     /geo_loc_name="South Africa: West Rand, Gauteng"
                     /lat_lon="26.06268 S 27.43202 E"
                     /altitude="1580 m"
                     /collection_date="2014-01"
                     /collected_by="Kulsum Kondiah"
     gene            complement(join(2978..4096,1..381))
                     /locus_tag="DN066_RS24865"
                     /old_locus_tag="DN066_24375"
     CDS             complement(join(2978..4096,1..381))
                     /locus_tag="DN066_RS24865"
                     /old_locus_tag="DN066_24375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_024145502.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGASVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEQDLPPGQRETLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGSLGLHDPNAPENRRALVTPSAL
                     PETKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPDAVLSHEPAYERDAAHGQPDVADHRPGLRAADSLKCGHSMVAGAADTRQLHDHP
                     GAEEHAGHAVREEQRRAAHDLRRGTTPLREGEPGRGTVRRGFELDDTGGEIAHDGTGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYATGERSAERARHGLEHAGAEFKRAAA
                     PVVVRLNDIEAHREQERAAQHQKALELERSQRQQEYDGPSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(371..694)
                     /locus_tag="DN066_RS24375"
                     /old_locus_tag="DN066_24380"
     CDS             complement(371..694)
                     /locus_tag="DN066_RS24375"
                     /old_locus_tag="DN066_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1333..1491
                     /locus_tag="DN066_RS24920"
                     /old_locus_tag="DN066_24390"
     CDS             1333..1491
                     /locus_tag="DN066_RS24920"
                     /old_locus_tag="DN066_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(1728..1997)
                     /locus_tag="DN066_RS24525"
                     /old_locus_tag="DN066_24395"
     CDS             complement(1728..1997)
                     /locus_tag="DN066_RS24525"
                     /old_locus_tag="DN066_24395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            2079..2312
                     /locus_tag="DN066_RS24395"
                     /old_locus_tag="DN066_24400"
     CDS             2079..2312
                     /locus_tag="DN066_RS24395"
                     /old_locus_tag="DN066_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2334..2561
                     /locus_tag="DN066_RS24400"
                     /old_locus_tag="DN066_24405"
     CDS             2334..2561
                     /locus_tag="DN066_RS24400"
                     /old_locus_tag="DN066_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(803..881)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          957..1445
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(2..381)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.638"
                     /coverage="100.0"
                     /evalue="6.57e-74"
     Typing          complement(2982..4096)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.172"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgcggtca tagtatggct ggagccgttt gccggtcagc agctccgtgt tcgggatcag
       61 gaagttcagc tccagccgcc ccttgtcccg gtgctcaacc cacagcacgc tgtactggtc
      121 tttatcgagt ccgggcatca gaacccgctc gaagctcgcc atcagcgttt cacgctgccc
      181 gggcggtaag tcctgttcgg caaaggacag caccccggag gtgtactttt tggcgtaggg
      241 cgaggcgtcg ataagctccc ggacttcctc cggcttcccc tgaagaacgc tggcaccgtc
      301 gcgctggcgg tctttcccca gcagataatc aaccgggccg gcaccgcctc cgcgtccccg
      361 gggatgaaac ttaacgatca tcgtcagccc ccttttccag tacggcatgc cgcagccgct
      421 cgagtccggc atcgatggcc atcagcgcgg cgacgacctg cacgcggtcg agaccgctac
      481 tgccaccggc gttaacctgg cgggcaatct ggttgaggtt gttccccatg ccggcaagct
      541 gacgaagcag cgccggcgag atcgagggaa gtttgccggc ccgggcgggc ttctcgtcca
      601 ggcacgtctg ccgcatccag gccgcgagct gtttaccgtc gcagcgttcc agcagacggc
      661 ggtgctcatc ttctgttacc cacatcgtga gcattttgct gcgtttgtct gccaatccgg
      721 cctcctgata acgccgggac gggcgggaaa tcttttcccg gtcggcgcgg tgttgggcaa
      781 gccgcaggcg cgtcagtggc atttagcggg tttcggggcg cagccctgaa ccagtcacgt
      841 agcgctagcg gagtgtatac tggcttacta tggtggcacg acgggaatcg ggatgaaata
      901 gtcagtcatg aaaactgtga gatgaggctc aggtgcgtaa gaggctgatg cctgtcccgg
      961 tggatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
     1021 ggtacggact cacggataaa gacgtggcgg ggaaatacgt gaatgcagga ggggaagaaa
     1081 ggatgcgccg ctgccggttt tccataggct ccgcccccct gacaagcatc acagaatctg
     1141 acgctcaaat cagtggtggc gaaacccgat gcggcttaaa gataccaggc gtttccccct
     1201 gatggctccc tcgtgctccc ccgcattcct gcctttcggt ttaccggtgt cattccgctg
     1261 ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgcgaaggc agttcgctcc
     1321 aggattgact gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggaaagacac gaaaaatcgc tactggcagc agccattagt
     1441 aactggaggt atacgagaga caaattaaga ggtcttgaag tatgggcctg agagaggcta
     1501 cactgaaaga acagttttgg tttctgcggt ctcgtatacc cagttacctg gttaagcagt
     1561 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgttttcaga
     1621 gcaagagatt acgactgtaa cgacaggatc acaagaagat catttactca gagagactaa
     1681 ctaccactat gtttgcctat caacatactt aggaaacatt acttgagtta acgactccac
     1741 cttactttta gtatctcgca atgaatcttc cgaccgccgt tgtgttgaag ttctattgcg
     1801 ccaccggggt ataaatcact ttcgtttaac tcaattaaaa gctcgttctt gtgagcatcc
     1861 tctaaataaa ggtaataaca cccaccataa aggttctgcg tatctggcac ggcaacaaaa
     1921 gatttctttc cgaacagtaa aacagcctct ttttctccat gctctgtcag caataatcga
     1981 aattcttgtc cattcaatcc tcttttcgca tgaaaaaacg acctatactt agatggttag
     2041 tgaccatatc cactttacct ttaagcaggg aacctattat gaataaaaaa acgctgttgg
     2101 cggccatgat ggtcgcgttg gtgacctttg ccgctgctgg atgttcttca aaccaagaaa
     2161 taaaaaccac agatgatcgc actatcgtta cagacggcaa acccaaagtt gacagtgata
     2221 cgggattagt atcctacaaa gatgcccata caggccgaac cgaacagatc aaccgtgaca
     2281 aaattaccaa catgagtgaa ctagacaact gatacggata aaaggagctg gctttgaaaa
     2341 agatttttgt ttcagtgttc gcagcggcag ttgcgctttc agccctaacc ggctgcacgc
     2401 gcacaagcta tgccatccac accaacgacg ggcgtacaat tgtcagcgac ggcaagccta
     2461 cagaatcaga ctcaggcctg ctgggctata aagacgcaaa tggtgtcaaa cagcagatca
     2521 ataaagccga cgttaaagaa gtctctgaaa ttccgcattg aggcatcgct ctctccgaaa
     2581 agggccgcta atgcggccct tttctttatc tgccccaaca acaggcaaaa aaagcccccg
     2641 ctgggggcca aatacctgca gtggctggat agtactgcat tcctgatgaa ggagacgaat
     2701 aagtatgggc cggatttttg cgtctgcagg ctcggtcgcc acaatttttc cgtacccgcc
     2761 aggcatcatg cttttaccat aacatccctt gcccgcacct ctccggacgg gttcagcgcg
     2821 aatgcggcgt cagtcctgca aaaccgacag taaaccaccc cgcaaaccgt gcattctgcg
     2881 cctgataaat ctcacccgac tgcatagcca tgcagccggg tgaatggcac cgggtttcgg
     2941 cgcagatcct caatgaaagc cccgttttat ccggttatca cagcgacgga ccgtcatact
     3001 cctgctgacg ctgcgatcgt tccagctcca gcgctttctg gtgctgcgct gcgcgctcct
     3061 gctcccggtg tgcctcgatg tcgttcagcc ttacgacaac cggcgcagct gctcgcttaa
     3121 actcagcacc tgcgtgctca agtccgtgac gcgctcgctc agcgctgcgt tctcccgttg
     3181 cataagacca cacatcgtcc gccattccgc gaaggcgctc tcccactcgt ccagcctttt
     3241 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca gttccgtcat
     3301 gtgctatttc cccgccagta tcatccagct cgaatcctct ccgaaccgtc ccgcgtccgg
     3361 gttcaccctc acgcagcggc gtcgttcctc tccgcaggtc gtgagctgca cgccgctgtt
     3421 cctctcggac agcatggcct gcgtgctctt ctgctcccgg atggtcgtgt aattgtcgag
     3481 tatctgctgc ccctgccacc atagaatgcc cgcacttgag gctatcagca gcacggagac
     3541 caggacgatg gtcagccacg tctggctgac catgcgcagc atccctttcg tacgctggct
     3601 catggctgag gacagcatcc ggtcgtggtc gtggatggcg gcgctgattc tcttctcgct
     3661 ttcgttcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt tcagcatctc
     3721 gccggtagtc tgctgctgca ctttcgattt ctgctctaag tccttcgcca gcgttaaaag
     3781 actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc
     3841 tgatgctgct ttttgtggtg cgaaccacct caaaacctgc gctttccagc gcctcagtga
     3901 cgtcctgacg cgttttcagc tccccggacg aggctaaggc cagtaaaccc cgcgtaatcg
     3961 cctgagcggc ttcctgcttc gtttccggca gcgcggacgg cgtgaccagt gcccgccggt
     4021 tttccggcgc gttcgggtcg tgcagcccca gactgccgtt cacgatggtc tgccaggcat
     4081 caatgcgagg acggtc
//
