LOCUS       NZ_CP030008             3458 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Enterobacter hormaechei subsp. xiangfangensis strain Pb204 plasmid
            pPb204001, complete sequence.
ACCESSION   NZ_CP030008
VERSION     NZ_CP030008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09389154
            Assembly: GCF_003254805.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. xiangfangensis (Enterobacter
            xiangfangensis)
  ORGANISM  Enterobacter hormaechei subsp. xiangfangensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ho,N.R., Kondiah,K. and De Maayer,P.
  TITLE     Complete genome of Enterobacter xiangfangensis Pb204: a South
            African strain capable of synthesising gold nanoparticles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Ho,N.R., Kondiah,K. and De Maayer,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2018) School of Molecular and Cell Biology,
            University of the Witwatersrand, Private Bag 3, University of the
            Witwatersrand, Johannesburg 2050, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-DEC-2017
            Assembly Method                   :: SPAdes v. 3.9.0
            Assembly Name                     :: FINAL-Pb204N1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 389.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003254805.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 03:02:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,647
            Genes (coding)                    :: 4,575
            CDSs (with protein)               :: 4,575
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 49 of 72
            Pseudo Genes (internal stop)      :: 9 of 72
            Pseudo Genes (multiple problems)  :: 14 of 72
            Pseudo Genes (short protein)      :: 1 of 72
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030008.1.
            Bacteria and source DNA available from Kondiah Kulsum, Department
            of Biotechnology and Food Technology, University of Johannesburg,
            South Africa.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterobacter hormaechei subsp. xiangfangensis"
                     /mol_type="genomic DNA"
                     /strain="Pb204"
                     /isolation_source="acid mine decant and tailings from
                     uranium mine"
                     /sub_species="xiangfangensis"
                     /db_xref="taxon:1296536"
                     /plasmid="pPb204001"
                     /geo_loc_name="South Africa: West Rand, Gauteng"
                     /lat_lon="26.06268 S 27.43202 E"
                     /altitude="1580 m"
                     /collection_date="2014-01"
                     /collected_by="Kulsum Kondiah"
     gene            complement(248..1408)
                     /gene="mobQ"
                     /locus_tag="DN066_RS24355"
                     /old_locus_tag="DN066_24360"
     CDS             complement(248..1408)
                     /gene="mobQ"
                     /locus_tag="DN066_RS24355"
                     /old_locus_tag="DN066_24360"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057520085.1"
                     /translation="MAIYHLSFKLCKRSDGKSSVYLAAYQNRNKIKDNRTGTVWNYSKK
                     EGFDGSLILAPPQAPGWLVKSSEALWNEVERTERQKNGQTARYFDVALPVELDDKQKKD
                     LLIKYCQDNFVDEGMIADIAFHDLNSKNPHAHVMLTMKNVGPEGFLTKNREWNDKKCLE
                     AWRQNWEVITNEALKKYGHKSRIDSRSLEEQKEEADRKAKVAKTPQAKAAWIAKSIRLD
                     RTPMTRIHRHNWKAGQKLRKLEQEERKILYKKASLTYHQITKPIEPEQSMKIEKSQIAQ
                     KIKSIFETAKNKLNSKIADFFKPKPKKETPEKFVSSYEIDELGNHILKKEAENWDQKNN
                     DKIKKQVREKKEKEESERLRLKEEDEKLKNSSKPIQQNNNHRKLKR"
                     /name="mobQ"
     gene            1646..1897
                     /locus_tag="DN066_RS24470"
     CDS             1646..1897
                     /locus_tag="DN066_RS24470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123912605.1"
                     /translation="MEYKNIYKKCCKCRKETEHEREEAYVMEKSLSKDDWEYSPSELAS
                     YMNGEFYDDYEEVLRLFDTCCVCGDKSYGSWSSEELTR"
                     /name="hypothetical protein"
     gene            1913..2236
                     /locus_tag="DN066_RS24360"
                     /old_locus_tag="DN066_24365"
     CDS             1913..2236
                     /locus_tag="DN066_RS24360"
                     /old_locus_tag="DN066_24365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057520086.1"
                     /translation="MENIFTVHIEEGFPISSIPALIDLFLRHYHCNNFDIDKALEATRE
                     NIADNLGALTKMKKPKVDIEKIMVEDIMSGLKTMVEEMGVLELYKIHFEINEMGMSWGV
                     DNE"
                     /name="hypothetical protein"
     gene            2229..2498
                     /locus_tag="DN066_RS24365"
                     /old_locus_tag="DN066_24370"
     CDS             2229..2498
                     /locus_tag="DN066_RS24365"
                     /old_locus_tag="DN066_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_057520087.1"
                     /translation="MSKSTAQQIAELQNRLQALRTKKSRQDKREETRQKIILGAEVAKV
                     LNSKPEEINKALVLGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     Typing          complement(252..1408)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tactaaagaa taaaagaaat atagttcgaa gaaaaaaatt ggcgacttgg attttttggt
       61 gaaattatga aaaaaaagaa attgcagaag aaaaaaagaa gaaataaaaa taagcatagt
      121 aaatttttta aaatgcttat gaagtcagtt aatgaagctg tgaaaattca caaaggtgag
      181 ttagagccag ccagagtaac aattttgaaa atgtagtata gtatattgta gtatatagca
      241 atatatacta tcgctttaat ttcctatgat tgttattttg ctgaattggc ttggagctat
      301 tttttaattt ctcatcctct tctttaagcc tcaacctttc ggattcttct ttttcttttt
      361 tctcacgaac ctgcttttta attttatcat tgtttttctg atcccaattt tcagcctctt
      421 ttttcaatat gtgatttcct aactcgtcaa tttcataact actgacaaat ttttcaggag
      481 tttctttttt gggtttaggc ttgaaaaagt cagcgatttt cgaatttaat ttatttttgg
      541 cagtttcgaa tatagatttt attttttgag ctatttgtga tttttctatt ttcatagatt
      601 gctcaggctc aattggttta gttatttggt gatatgttaa cgaagccttt ttatataaaa
      661 tcttcctttc ttcttgctcc aattttctta atttctgccc agctttccaa ttatgcctgt
      721 gaatgcgggt catcggcgtt ctgtccagac ggatagattt agcgatccaa gcagcttttg
      781 cctgtggagt ttttgcaact tttgcttttc tatcagcctc ttctttttgt tcttccaatg
      841 aacgggaatc aatacgggat ttatgaccat atttttttaa tgcctcattg gtaataacct
      901 cccaattttg cctccaggct tctaaacatt tcttatcatt ccattctctg ttttttgtta
      961 aaaaaccttc tggcccaaca tttttcattg ttaacattac atgggcatgg ggatttttac
     1021 tatttagatc gtggaatgca atatcggcaa tcattccctc atctacgaaa ttatcctgac
     1081 agtattttat taaaaggtct tttttttgtt tgtcgtccag ctccaccggc aaggcgacat
     1141 cgaagtacct ggcagtctgt ccgttcttct gtcgttctgt cctctcaacc tcattccata
     1201 gggcctcaga cgacttcaca agccatcctg gtgcttgtgg tggtgccagg attaacgaac
     1261 cgtcaaagcc ttcttttttg ctgtaattcc atactgtacc ggttctatta tctttgattt
     1321 tatttctatt ctgatatgca gccaaatata cggaagattt accatctgat cttttgcata
     1381 atttaaaact taaatgatat atagccatag gctaccccct tacttttttt atcttttgaa
     1441 ttttgggttt tagccccgca gggcccacac actcggaacg agtgtataag tgggcattac
     1501 taatattatt ttttcgattg catctcaaaa taataaagta ataaacccag gcttcgccca
     1561 atttttatat aaatattatg taatatattt gactaaagta aataatggag gtagataata
     1621 ataaaataaa taatcaggag gttgtatgga atataaaaat atatataaaa agtgttgtaa
     1681 gtgtaggaaa gaaacagaac acgaaagaga agaggcttat gtaatggaaa aatctttaag
     1741 caaagacgat tgggaatatt cgccgtctga attggcttct tatatgaatg gtgagtttta
     1801 tgacgattat gaagaggttt taaggctatt tgatacctgt tgtgtatgtg gtgataaatc
     1861 ttatggtagt tggtcaagtg aagaattaac aagataaaat aatggaggtt gtatggaaaa
     1921 tatatttaca gttcacattg aggaaggttt cccaatttca tctattccgg ctttaatcga
     1981 tttgttttta cgtcattatc attgtaataa ctttgatata gataaggctc ttgaggctac
     2041 aagggaaaac atagccgata atttaggggc attaactaaa atgaaaaagc caaaagtcga
     2101 tattgaaaag attatggttg aagatattat gtcagggtta aaaacaatgg ttgaagaaat
     2161 gggagtgtta gaattatata aaattcattt tgaaataaac gaaatgggaa tgagttgggg
     2221 ggttgataat gagtaaatca acagcacagc agatcgcaga attacaaaat agactacaag
     2281 cattaagaac aaaaaaatcc agacaagata aaagagaaga aacaagacaa aaaataatac
     2341 tcggtgcaga agtggctaag gttttaaata gtaaaccaga agaaataaat aaagctcttg
     2401 tattaggttt gttaagtaaa atcaatgaat tagacgaaga agagaaaaga gattttacac
     2461 ggatcggaat ggatatattg cagcaatgga aatcgtaaac cgtgaacagt gaagaaccag
     2521 ccgcttttgg ctggtttttt actgtgaagg gttgcagccg taggctgaag tctttaaaac
     2581 gctctcagag ggcctctggc gattcctggg tgggtttgtt gtttgttcgt gtcagagtgt
     2641 ccctcagcct cggatatgaa gcccggaacg ggaaaagggg aggatcttcc gtcgaggttt
     2701 ttggatcaaa ttttttgaac aactattgac agaacaagaa gataagttat aacaggtaca
     2761 gatacaacaa aaccaccgtt gaggtggttt tgagggactc gaaaaggctg gaaccttttt
     2821 aagtcttgta atccagttgg ttcagacaac aagattacga tttgtaagta agaagttaac
     2881 tcacaattcg caaaaagtca aatacccctc gattgcaatt cccaaaaaaa atccatccgt
     2941 ttcaagtcct taagcataca tagtttgttt aaaccgattg tgtccggtgt ttggtgtgga
     3001 gccattgatc cgagtggtca atatgtgatt gttcgccaaa cagtgtatgt aggcagaaac
     3061 ggggagtgct acgaactgcc aaaccgggaa cgaagtttct aaggtgcttt ataagtgatc
     3121 aggtaggcta tgagaaagcc cagccaaacc ctgtaagggg ttgaaatggg gttagatcat
     3181 tatattagtg ctatgaaacg accagggggg tgtagcaggg tgccaatggc tggaaacagt
     3241 cataaagctc taagtcctgg cttctgtggg tcaagcaaaa aaccgctgat accataggat
     3301 cgtcaacctg attatttcgt tgaaaaataa cgaaaaatcg gggaggcgaa actatgggtc
     3361 aagcccttca ggggttgacc catacaggcg aagcccccag acaagcaata cctttcctgt
     3421 tatattgtgc gaagcattaa agaaatattg taaaaccc
//
