LOCUS       NZ_CP029708             4440 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Pseudomonas aeruginosa strain K34-7 plasmid pK34-7-1, complete
            sequence.
ACCESSION   NZ_CP029708
VERSION     NZ_CP029708.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08954392
            Assembly: GCF_003206535.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Taiaroa,G., Samuelsen,O., Kristensen,T., Okstad,O.A. and Heikal,A.
  TITLE     Complete Genome Sequence of Pseudomonas aeruginosa K34-7: a
            Carbapenem-Resistant Isolate of the High-Risk Sequence Type 233
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Taiaroa,G., Samuelsen,O., Kristensen,T., Okstad,O.A. and Heikal,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2018) School of Pharmacy, University of Oslo, Sem
            Saelands vei 3, Oslo 0371, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2018
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003206535.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 09:05:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,635
            CDSs (total)                      :: 6,554
            Genes (coding)                    :: 6,494
            CDSs (with protein)               :: 6,494
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 18 of 60
            Pseudo Genes (incomplete)         :: 41 of 60
            Pseudo Genes (internal stop)      :: 5 of 60
            Pseudo Genes (multiple problems)  :: 4 of 60
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029708.1.
            Bacteria and source DNA available from Orjan Samuelsen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="K34-7"
                     /isolation_source="tracheal secretions"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pK34-7-1"
                     /geo_loc_name="Norway: Olso"
                     /lat_lon="59.93 N 10.74 E"
                     /collection_date="2006"
                     /collected_by="Ullevaal University Hospital"
     gene            154..1098
                     /locus_tag="DCS61_RS33915"
                     /old_locus_tag="DCS61_33915"
     CDS             154..1098
                     /locus_tag="DCS61_RS33915"
                     /old_locus_tag="DCS61_33915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1260..2639
                     /gene="tet(K)"
                     /locus_tag="DCS61_RS33920"
                     /old_locus_tag="DCS61_33920"
     ARG             1261..2636
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2826..4067
                     /gene="mobV"
                     /locus_tag="DCS61_RS33925"
                     /old_locus_tag="DCS61_33925"
     CDS             2826..4067
                     /gene="mobV"
                     /locus_tag="DCS61_RS33925"
                     /old_locus_tag="DCS61_33925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     Typing          155..1098
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2827..4064
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtttgtgaa cgacatcatt caaagaaaaa aacactgagt tgtttttata atcttgtata
       61 tttagatatt aaacgatatt taaatataca tcaagatata tatttgggtg agcgattcct
      121 taaacgaaat tgagattaag gagtcgattt tttatgtata aaaacaatca tgcaaatcat
      181 tcaaatcatt tggaaaatca cgatttagac aatttttcta aaaccggcta ctctaatagc
      241 cggttggacg cacatactgt gtgcatatct gatccaaaat taagttttga tgcaatgacg
      301 atcgttggaa atctcaaccg agacaacgct caagcccttt ctaaatttat gagtgtagag
      361 ccccaaataa gactttggga tattcttcaa acaaagttta aagctaaagc acttcaagaa
      421 aaagtttata ttgaatatga caaagtgaaa gcagatagtt gggatagacg taatatgcgt
      481 attgaattta atccaaacaa acttacacga gatgaaatga tttggttaaa acaaaatata
      541 ataagctaca tggaagatga cggttttaca agattagatt tagcctttga ttttgaagat
      601 gatttgagtg actactatgc aatgtctgat aaagcagtta agaaaactat tttttatggt
      661 cgtaatggta agccagaaac aaaatatttt ggcgtgagag atagtaatag atttattaga
      721 atttataata aaaagcaaga acgtaaagat aatgcagatg ctgaagttat gtctgaacat
      781 ttatggcgtg tagaaatcga acttaaaaga gatatggtgg attactggaa tgattgcttt
      841 agtgatttac atatcttgca accagattgg aaaactatcc aacgcactgc ggatagagca
      901 atagttttta tgttattgag tgatgaagaa gaatggggaa agcttcacag aaattctaga
      961 acaaaatata agaatttgat aaaagaaatt tcgccagtcg atttaacgga cttaatgaaa
     1021 tcgactttaa aagcgaacga aaaacaattg caaaaacaaa tcgatttttg gcaacatgaa
     1081 tttaaatttt ggaaatagtg tacatattaa tattactgaa caaaaatgat atatttaaac
     1141 tattctaatt taggaggatt tttttatgaa gtgtctattt aaaaatttgg ggaatttata
     1201 tgaggtgaaa gaataattta cccctataaa ctttagtcac ctcaagtaaa gaggtaaaat
     1261 tgtttagttt atataaaaaa tttaaaggtt tgttttatag cgttttattt tggctttgta
     1321 ttctttcatt ttttagtgta ttaaatgaaa tggttttaaa tgtttcttta cctgatattg
     1381 caaatcattt taatactact cctggaatta caaactgggt aaacactgca tatatgttaa
     1441 ctttttcgat aggaacagca gtatatggaa aattatctga ttatataaat ataaaaaaat
     1501 tgttaattat tggtattagt ttgagctgtc ttggttcatt gattgctttt attggtcaca
     1561 atcacttttt tattttgatt tttggtaggt tagtacaagg agtaggatct gctgcattcc
     1621 cttcactgat tatggtggtt gtagctagaa atattacaag aaaaaaacaa ggcaaagcct
     1681 ttggttttat aggatcaatt gtagctttag gtgaagggtt aggtccttca atagggggaa
     1741 taatagcaca ttatattcat tggtcttacc tacttatact tcctatgatt acaatagtaa
     1801 ctataccttt tcttattaaa gtaatggtac ctggtaaatc aacaaaaaat acattagata
     1861 tcgtaggtat tgttttaatg tctataagta ttatatgttt tatgttattt acgacaaatt
     1921 ataattggac ttttttaata ctcttcacaa tcttttttgt gatttttatt aaacatattt
     1981 caagagtttc taaccctttt attaatccta aactagggaa aaacattccg tttatgcttg
     2041 gtttgttttc tggtgggcta atattttcta tagtagctgg ttttatatca atggtgcctt
     2101 atatgatgaa aactatttat catgtaaatg tagcgacaat aggtaatagt gttatttttc
     2161 ctggaaccat gagtgttatt gtttttggtt attttggtgg ttttttagtg gatagaaaag
     2221 gatcattatt tgtttttatt ttaggatcat tgtctatctc tataagtttt ttaactattg
     2281 cattttttgt tgagtttagt atgtggttga ctacttttat gtttatattt gttatgggcg
     2341 gattatcttt tactaaaaca gttatatcaa aaatagtatc aagtagtctt tctgaagaag
     2401 aagttgcttc tggaatgagt ttgctaaatt tcacaagttt tttatcagag ggaacaggta
     2461 tagcaattgt aggaggttta ttgtcactac aattgattaa tcgtaaacta gttctggaat
     2521 ttataaatta ttcttctgga gtgtatagta atattcttgt agccatggct atccttatta
     2581 ttttatgttg tcttttgacg attattgtat ttaaacgttc tgaaaagcag tttgaatagt
     2641 tatattatat tttggtttag aactatgagt ggctagcatt ttgccactca ttttttgcgt
     2701 tagcaaaaac aggtttaagc ctcgcagagc acacgtatta acgacttatt gtaaaataag
     2761 tctagtgtgt tagacttaaa ctattaaata cacatgaaac ctttgtgctt aggagtgatt
     2821 tttatatgtc ttattccatt gttagagttt caaaagttaa atctggaaca aatacaacgg
     2881 gcatacaaaa acatgttcaa agagaaaata ataattatga aaatgaagat atagaccata
     2941 gtaaaactta cttaaattat gatttggtaa atgctaataa acagaatttt aataacttga
     3001 ttgatgaaaa aatcgaacag aattatacag gcaaaagaaa aattagaaca gacgcgatta
     3061 aacacattga tggtttaatt acatcagaca atgatttctt tgataatcaa acgccagaag
     3121 atacaaagca gttttttgaa tatgctaaag agtttttaga acaagaatac ggtaaagata
     3181 atttattata tgcaacagtt cacatggacg aaaaaacacc acatatgcat tatggcgttg
     3241 ttccaataac tgatgatggt cgtttaagtg ctaaagaagt tgtaggtaat aaaaaagctt
     3301 taacagcgtt tcaagataga tttaatgagc atgttaaaca acgaggatat gatttagaac
     3361 gtgggcaatc aagacaagta acaaatgcta aacatgagca aataagtcag tataaacaaa
     3421 aaacagaata tcataagcaa gaatatgaac gtgagagcca aaaaacagac catataaagc
     3481 aaaagaacga taaattaatg caagagtacc aaaaatcgtt aaatacgctt aaaaagccta
     3541 taaatgttcc gtatgagcaa gaaactgaaa aagtaggtgg tttatttagc aaagaaatac
     3601 aagaaactgg aaatgttgta ataagccaaa aagatttcaa tgaatttcag aaacagataa
     3661 aagctgctca agatatttcg gaagattacg agtatataaa gtctggtaga gccttagatg
     3721 ataaagataa ggaaatacga gagaaagatg atttattaaa taaagcagtt gagcgtattg
     3781 aaaacgcaga cgataatttt aaccaacttt acgaaaatgc aaagccactt aaagagaata
     3841 tagaaatagc gttaaagctt ttaaaaatct tactaaaaga gttagaacga gttttaggaa
     3901 gaaatacctt tgcggaaaga gttaataagt taacagaaga tgaaccaaaa ctaaatggtt
     3961 tagcaggaaa cttagataaa aaaatgaatc cagaattata ttcagaacag gaacagcaac
     4021 aagaacaaca aaagaatcaa aaacgagata gaggtatgca cttatagaac atgcatttat
     4081 gccgagaaaa cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt
     4141 tggacttgaa ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt
     4201 aggttccgac caataaggaa attggaataa agcaataaaa ggagttgaag aaatgaaatt
     4261 cagagaagcc tttgagaatt ttataacaag taagtatgta cttggtgttt tagtagtttt
     4321 aactgtttac cagataatac aaatgcttaa ataaaaaaag acttgatctg attagaccaa
     4381 gtcttttgat agtgttatat taataacaaa ataaaaagga gtcgctcacg tcctgaccaa
//
