LOCUS       NZ_CP029676             3127 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Staphylococcus aureus strain AR_0216 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP029676
VERSION     NZ_CP029676.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04901606
            Assembly: GCF_003193725.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0; HGAP v. 3.0
            Genome Coverage                   :: 12.77x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003193725.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 03:33:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,910
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 47 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 18 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029676.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="AR_0216"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
     gene            7..315
                     /locus_tag="CSB72_RS15655"
                     /old_locus_tag="CSB72_2872"
     CDS             7..315
                     /locus_tag="CSB72_RS15655"
                     /old_locus_tag="CSB72_2872"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162564141.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKKTT"
                     /name="hypothetical protein"
     gene            complement(916..1104)
                     /locus_tag="CSB72_RS00010"
                     /old_locus_tag="CSB72_2875"
     CDS             complement(916..1104)
                     /locus_tag="CSB72_RS00010"
                     /old_locus_tag="CSB72_2875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1687..2682)
                     /locus_tag="CSB72_RS00020"
                     /old_locus_tag="CSB72_2876"
     CDS             complement(1687..2682)
                     /locus_tag="CSB72_RS00020"
                     /old_locus_tag="CSB72_2876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1453..1617)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1688..2682)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttacaatga atgcaattca aaacagtata tcactaagaa agattttagt agtatcttta
       61 agtattttaa ccatactttc aatggtcttg gattttaatt tcaaagaagc tcaagcccaa
      121 aacaaaaata acattgcaga taaaaacgtt gaaactttaa acgaaaaaga aattgaaaaa
      181 gaactaaaat acatatatgg caaaattatt attttagata aagacggaac agctaaagat
      241 gttaatctag aaaatgccaa atctcgatat ggatatgttc ctgaaggatt ccaaaaacta
      301 aaaaaaacga catagagaat aaaaagaaaa ctagttcaat atcacctaga gcagttggtg
      361 ggaactacaa aaatagcggt gactgtttct attctgaagt tttaaactca tatggagaac
      421 ttttaactgg aaacataatc acagcagttt ttgaagatgt taaagccaaa aacttaaagg
      481 gcgtagcaaa aaaattagct aaaattggga taaaaggaaa cctagctgga atcgcagcta
      541 ctatggtttc gaaggatgta caatgtaatc ttaaatacgg gttactttag atactattta
      601 ttccaattgc tttattgacg ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa
      661 tggtcggctt aatagctcac gctatgccga cattcgtctg caagtttagt taagggttct
      721 tctcaacatc aataaatttt ctcggcataa atgcgtcgtc tattttttat tatcttcatg
      781 ataatcatat ttttcttcca tctcgtcaaa agcatcatct ttatagaaaa acaaaaagta
      841 agcaacaaca ccaataggaa taaaaaaaca gtgtgaaaac aagaagaaac tcgtttcaaa
      901 atcccctcaa atattctaat tattcttttt gaaattcata accttaacaa ccaaataaat
      961 agcatttatt cccatcaaaa taacatacgg aattaatata tataaatttt ccaaatgaga
     1021 tataaaaatt aatccaagta aaaagaaaac atttaaaata gttgctataa cgtaaccttc
     1081 atctaacact gtgtttttat tcaaaataaa cactcctttt ttaaaaaact ttatcacaag
     1141 acttactcaa attataaata cctttatttt gagttttaag ggggtatttt aaaggtttag
     1201 ggggtatcat ataggttttt aacttaaaaa tatatacaag ctcttaaaac gcaaatatga
     1261 gccaaataaa tatatggtaa tttcacaaac aaaaatttca gcaaaaccag tgactaattt
     1321 tttaacactg cccatttaca tgcaaattaa aaattagcat aaaatccttt aaaacttcca
     1381 catgtttttc taaattcctg cagggcgttt tttatcaaaa atcgagaatt tttaattttt
     1441 ctctaaaaac tccgtaagag cataaggaga atttgctatc attcaaaaat gataggtctg
     1501 gcaaagccag aaattccagg ggttttaagg tattttaaag tgatatcata caaagctgta
     1561 ctgtcttatt tttgtgacac aatatattgc gtttgtctta tttttgtgac actacataca
     1621 gcatttgtca caaaaataag acagtacttt tttataaaaa atcaacaaaa aagacattag
     1681 tcaacactaa tgtcttaaat aataattttg cttttcgaaa ttccaaattg cggtaattga
     1741 atgtgctttt tcttcttcgt ctgttgtttt atcttcatca cttgcattaa tcaaattacc
     1801 atcttcgaca tcgtctaagt ttaaaatttt atgtttttgt ttaagcaatc caccataact
     1861 taacatacgt tttcgataca aacctttttc taaatcactt acaatttctt gatttttttc
     1921 atcatcatca gttaaaaaat cagatgattt aaccgaatat tttgaggtct ctttgattgc
     1981 cgattcaatg tctttatcgc cttttctatt cggtttgatc gctttaacat tagcaacagg
     2041 tcgatagtca acttgtaatg ctctttgcca taaattaacc cattcttctt gagttatata
     2101 attctctttt tttctaaaat atgcattttc aacacataac aaaacatgca tgtgctgatt
     2161 ataactaccg tcatttttat taacggtaac ttctgttgaa cgcataaatc caacaagatt
     2221 ttgtttaacc tttttatatc tactcaacct atcaaatgct ttagttagat gcttcaaact
     2281 ttgttctaaa gtatctccat ctatcgcatt ttttgttgaa agtgttaaaa acaaccaacg
     2341 tgcttttggc ttttccttaa ttactttttc aatcactttt tgagcttgat aactattttt
     2401 catagcacgt ctccaattac aaaccggaca taattttgat ttacaaaacc atgtcttatg
     2461 taacttcaaa tagccttcat cagttggctt aaattctaaa atattaccac attgttttac
     2521 attaaaagcc tttttgattt ttaaaatttc tagtatatct gcatagctta cattatctat
     2581 tttcttttct ctccatgggc gttgtttccc acttttcgtc atatctttta acgttttatt
     2641 atcttgattt tcggttatac ttctagtagt attatattgc atataaaaag acccccagta
     2701 ttatttaaga ttcgacacct taataatata actggggttt tttattgtca aattttcctt
     2761 tcacgcacaa aaacacgttt aaacactgta atatcaacag ttacagctat atatataaaa
     2821 tcgagttatt tctatatagt atcaagacaa gaagaaactc gttcacactc gtttcaaaaa
     2881 acaacctatt ccttcttctt tttatgaccc tctaaaccta aataaattaa tgtgaaaacc
     2941 aatgaataaa taaaactttc taaaaagtca aaaggattat caaataaaat cgaattaaca
     3001 aaattaaaaa ttaaaaatac agataataaa ataatcaaaa agtaaaaaaa cttattcata
     3061 ttaaactcct ttgtttttat atgttaatat tatattgaat tcatttaaat aacaaaacaa
     3121 gggggca
//
