LOCUS       NZ_CP029668             3125 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Staphylococcus aureus strain AR_0223 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP029668
VERSION     NZ_CP029668.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04901613
            Assembly: GCF_003193685.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 28.37x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003193685.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 03:23:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,815
            Genes (coding)                    :: 2,731
            CDSs (with protein)               :: 2,731
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 47 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 16 of 84
            Pseudo Genes (multiple problems)  :: 18 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029668.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="AR_0223"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
     gene            complement(242..430)
                     /locus_tag="CSB79_RS00005"
                     /old_locus_tag="CSB79_0002"
     CDS             complement(242..430)
                     /locus_tag="CSB79_RS00005"
                     /old_locus_tag="CSB79_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1013..2008)
                     /locus_tag="CSB79_RS00015"
                     /old_locus_tag="CSB79_0003"
     CDS             complement(1013..2008)
                     /locus_tag="CSB79_RS00015"
                     /old_locus_tag="CSB79_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2460..3041
                     /locus_tag="CSB79_RS00020"
                     /old_locus_tag="CSB79_0005"
     CDS             2460..3041
                     /locus_tag="CSB79_RS00020"
                     /old_locus_tag="CSB79_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          complement(779..943)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1014..2008)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata
       61 aattttctcg gcataaatgc gtcgtctatt ttttattatc ttcatgataa tcatattttt
      121 cttccatctc gtcaaaagca tcatctttat agaaaaacaa aaagtaagca acaacaccaa
      181 taggaataaa aaaacagtgt gaaaacaaga agaaactcgt ttcaaaatcc cctcaaatat
      241 tctaattatt ctttttgaaa ttcataacct taacaaccaa ataaatagca tttattccca
      301 tcaaaataac atacggaatt aatatatata aattttccaa atgagatata aaaattaatc
      361 caagtaaaaa gaaaacattt aaaatagttg ctataacgta accttcatct aacactgtgt
      421 ttttattcaa aataaacact ccttttttaa aaaactttat cacaagactt actcaaatta
      481 taaatacctt tattttgagt tttaaggggg tattttaaag gtttaggggg tatcatatag
      541 gtttttaact taaaaatata tacaagctct taaaacgcaa atatgagcca aataaatata
      601 tggtaatttc acaaacaaaa atttcagcaa aaccagtgac taatttttta acactgccca
      661 tttacatgca aattaaaaat tagcataaaa tcctttaaaa cttccacatg tttttctaaa
      721 ttcctgcagg gcgtttttta tcaaaaatcg agaattttta atttttctct aaaaactccg
      781 taagagcata aggagaattt gctatcattc aaaaatgata ggtctggcaa agccagaaat
      841 tccaggggtt ttaaggtatt ttaaagtgat atcatacaaa gctgtactgt cttatttttg
      901 tgacacaata tattgcgttt gtcttatttt tgtgacacta catacagcat ttgtcacaaa
      961 aataagacag tactttttta taaaaaatca acaaaaaaga cattagtcaa cactaatgtc
     1021 ttaaataata attttgcttt tcgaaattcc aaattgcggt aattgaatgt gctttttctt
     1081 cttcgtctgt tgttttatct tcatcacttg cattaatcaa attaccatct tcgacatcgt
     1141 ctaagtttaa aattttatgt ttttgtttaa gcaatccacc ataacttaac atacgttttc
     1201 gatacaaacc tttttctaaa tcacttacaa tttcttgatt tttttcatca tcatcagtta
     1261 aaaaatcaga tgatttaacc gaatattttg aggtctcttt gattgccgat tcaatgtctt
     1321 tatcgccttt tctattcggt ttgatcgctt taacattagc aacaggtcga tagtcaactt
     1381 gtaatgctct ttgccataaa ttaacccatt cttcttgagt tatataattc tctttttttc
     1441 taaaatatgc attttcaaca cataacaaaa catgcatgtg ctgattataa ctaccgtcat
     1501 ttttattaac ggtaacttct gttgaacgca taaatccaac aagattttgt ttaacctttt
     1561 tatatctact caacctatca aatgctttag ttagatgctt caaactttgt tctaaagtat
     1621 ctccatctat cgcatttttt gttgaaagtg ttaaaaacaa ccaacgtgct tttggctttt
     1681 ccttaattac tttttcaatc actttttgag cttgataact atttttcata gcacgtctcc
     1741 aattacaaac cggacataat tttgatttac aaaaccatgt cttatgtaac ttcaaatagc
     1801 cttcatcagt tggcttaaat tctaaaatat taccacattg ttttacatta aaagcctttt
     1861 tgatttttaa aatttctagt atatctgcat agcttacatt atctattttc ttttctctcc
     1921 atgggcgttg tttcccactt ttcgtcatat cttttaacgt tttattatct tgattttcgg
     1981 ttatacttct agtagtatta tattgcatat aaaaagaccc ccagtattat ttaagattcg
     2041 acaccttaat aatataactg gggtttttta ttgtcaaatt ttcctttcac gcacaaaaac
     2101 acgtttaaac actgtaatat caacagttac agctatatat ataaaatcga gttatttcta
     2161 tatagtatca agacaagaag aaactcgttc acactcgttt caaaaaacaa cctattcctt
     2221 cttcttttta tgaccctcta aacctaaata aattaatgtg aaaaccaatg aataaataaa
     2281 actttctaaa aagtcaaaag gattatcaaa taaaatcgaa ttaacaaaat taaaaattaa
     2341 aaatacagat aataaaataa tcaaaaagta aaaaaactta ttcatattaa actcctttgt
     2401 ttttatatgt taatattata ttgaattcat ttaaataaca aaacaagggg gcatttacaa
     2461 tgaatgcaat tcaaaacagt atatcactaa gaaagatttt agtagtatct ttaagtattt
     2521 taaccatact ttcaatggtc ttggatttta atttcaaaga agctcaagcc caaaacaaaa
     2581 ataacattgc agataaaaac gttgaaactt taaacgaaaa agaaattgaa aaagaactaa
     2641 aatacatata tggcaaaatt attattttag ataaagacgg aacagctaaa gatgttaatc
     2701 tagaaaatgc caaatctcga tatggatatg ttcctgaagg attccaaaaa ctaaaaaacg
     2761 acatagagaa taaaaagaaa actagttcaa tatcacctag agcagttggt gggaactaca
     2821 aaaatagcgg tgactgtttc tattctgaag ttttaaactc atatggagaa cttttaactg
     2881 gaaacataat cacagcagtt tttgaagatg ttaaagccaa aaacttaaag ggcgtagcaa
     2941 aaaaattagc taaaattggg ataaaaggaa acctagctgg aatcgcagct actatggttt
     3001 cgaaggatgt acaatgtaat cttaaatacg ggttacttta gatactattt attccaattg
     3061 ctttattgac gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct
     3121 taata
//
