LOCUS       NZ_CP029648             2473 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Staphylococcus aureus strain AR_0472 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP029648
VERSION     NZ_CP029648.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291565
            Assembly: GCF_003193665.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 31.41x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003193665.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 03:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,835
            CDSs (total)                      :: 2,754
            Genes (coding)                    :: 2,685
            CDSs (with protein)               :: 2,685
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 35 of 69
            Pseudo Genes (incomplete)         :: 35 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 15 of 69
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029648.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="AR_0472"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
     gene            complement(738..1214)
                     /locus_tag="CSC57_RS00005"
                     /old_locus_tag="CSC57_0001"
     CDS             complement(738..1214)
                     /locus_tag="CSC57_RS00005"
                     /old_locus_tag="CSC57_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(1662..2396)
                     /gene="erm(C)"
                     /locus_tag="CSC57_RS00010"
                     /old_locus_tag="CSC57_0002"
     ARG             complement(1666..2396)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1663..2396)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(729..1214)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gataatgaac tgtgctgatt acaaaaatac taaaaatgcc catatttttt cctccttata
       61 aaattagtat aattatagca cgagctctga taaatatgaa catgatgagt gatcgttaaa
      121 tttatactgc aatctgatgc gattattgaa taaaagatat gagagattta tctagtttct
      181 ttttttacaa gaaaaaagaa agttcttaaa ggttttatac ttttggtcgt agagcacacg
      241 gtttaacgac ttaattacga agtaaataag tctagtgtgt tagactttaa tgttttttta
      301 aggcattagt gcatttaagc gtcagagcat ggctttatgc cgagaaaact attggttgga
      361 atggcgtgtg tgttagccaa agctttggcg agttggttgg gggtttcatg ggattaatcc
      421 catgaaagta ccaactcaac aacacactaa cgcctgttgg ttccaaccaa taggaaattg
      481 gaataagcaa ttagtataat gagagtataa tgttggtata acgttagtat aatgatgctt
      541 tttttcatta tattttttat gtactttaaa cctgcacgct tatgtgaatt agaaaaagct
      601 taatcgcatt tcatagattg acctcccaat aactacgtgg tgttattggg aggtcaatct
      661 atttcatttg cgttaagctt ttgatctaca ttaagcgtgc aggttttttg tctatttgta
      721 gacgatttta tgttatacta gtccttgaaa gaataataat cagataatgc attttcttgt
      781 ttttcatttg cctcttgctc aaagttccca aattcgagta agaggtattt ttgtttttgg
      841 tcgtcgcctc tcattagtag ttcagggttt aacattaata ctccagtttt tctttttata
      901 atatttcctt cttctaagat tttaagtgtt gttattactg tttgtagact tgttcctgta
      961 gcttttgcta tttctcttgt tgtagctatc attgtattgt tacttaagtg gacattatct
     1021 aggatatagt taacgatttt aagttttttt ccgccaatca tatctaacat acttattaat
     1081 tgcactatat atgcctttac gaagttacca gacgtttgtt tacggtataa cttgtctacc
     1141 tctatgactt ctccactttc ttcgtctatg agcctctgag agcctttata gactgttcca
     1201 tatctttctt tcatcttttt ctcactcctt attttaaact attctaacta tatcataact
     1261 gttctaaaaa aaaaagaaca tttgttaaaa gaaattagaa caaaatgagt gaaaaattag
     1321 aacaaacaaa ttccttataa accttatcat ctcaacctat attaagattt tacctagttg
     1381 aatcttcttt tctatataaa gcgtcggagc atatcagggg gttatctaac gtaaatgcta
     1441 cccttcggct cgctttcgct cggcattgac gtcagatact gcaccccctg aacccccatg
     1501 ctccaacagc aaaaaggaaa ctttttgctg cttttccgac gcttattcgc ttcgctcata
     1561 tttatataga aaagaagtga atgcgcaaaa gacataatcg attcacaaaa aataggtaca
     1621 cgaaaaacaa gttaagggat gcagtttatg catcccttaa cttacttatt aaataattta
     1681 tagctattga aaagagataa gaattgttca aagctaatat tgtttaaatc gtcaattcct
     1741 gcatgtttta aggaattgtt aaattgattt tttgtaaata ttttcttgta ttctttgtta
     1801 acccatttca taacgaaata attatacttc tgtttatctt tgtgtgatat tcttgatttt
     1861 tttctattta atctgataag tgagctattc actttaggtt taggatgaaa atattctctt
     1921 ggaaccatac ttaatataga aatatcaact tctgccatta aaaataatgc caatgagcgt
     1981 tttgtattta ataatctttt agcaaacccg tattccacga ttaaataaat ctcatcagct
     2041 atactatcaa aaacaatttt gcgtattata tccgtactta tgttataagg tatattacca
     2101 aatattttat aggattggtt tttaggaaat ttaaactgca atatatcctt gtttaaaact
     2161 tggaaattat cgtgatcaac aagtttattt tctgtagttt tgcataattt atggtctatt
     2221 tcaatggcag ttacgaaatt acacctctgt actaattcaa gggtaaaatg cccttttcct
     2281 gagccgattt caaagatatt atcatgttca tttaatctta tatttgtcat tattttatct
     2341 atattatgtt ttgaagtaat aaagttttga ctgtgtttta tatttttctc gttcattata
     2401 accctcttta atttggttat aatgaatttt gcttattaac gattcattat aaccacttat
     2461 tttttgtttg gtt
//
