LOCUS       NZ_CP029592             5251 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain DA33144 plasmid pDA33144-5, complete
            sequence.
ACCESSION   NZ_CP029592
VERSION     NZ_CP029592.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09245753
            Assembly: GCF_003181135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     High prevalence of unstable heteroresistance caused by gene
            amplification in clinical bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2018) Department of Medical Biochemistry and
            Microbiology, Uppsala University, Husargatan 3, Uppsala 75442,
            Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics workbench v. 11.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,442
            CDSs (total)                      :: 5,313
            Genes (coding)                    :: 5,184
            CDSs (with protein)               :: 5,184
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 61 of 129
            Pseudo Genes (internal stop)      :: 13 of 129
            Pseudo Genes (multiple problems)  :: 10 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029592.1.
            Bacteria and source DNA available from Dan I Andersson.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DA33144"
                     /serotype="ST16"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pDA33144-5"
                     /geo_loc_name="Sweden"
     gene            complement(join(5076..5251,1..145))
                     /locus_tag="DLJ67_RS27955"
                     /old_locus_tag="DLJ67_27955"
     CDS             complement(join(5076..5251,1..145))
                     /locus_tag="DLJ67_RS27955"
                     /old_locus_tag="DLJ67_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            782..943
                     /locus_tag="DLJ67_RS27965"
                     /old_locus_tag="DLJ67_27965"
     CDS             782..943
                     /locus_tag="DLJ67_RS27965"
                     /old_locus_tag="DLJ67_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(1096..1716)
                     /locus_tag="DLJ67_RS27970"
                     /old_locus_tag="DLJ67_27970"
     CDS             complement(1096..1716)
                     /locus_tag="DLJ67_RS27970"
                     /old_locus_tag="DLJ67_27970"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(1709..3064)
                     /locus_tag="DLJ67_RS27975"
                     /old_locus_tag="DLJ67_27975"
     CDS             complement(1709..3064)
                     /locus_tag="DLJ67_RS27975"
                     /old_locus_tag="DLJ67_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(3697..3909)
                     /locus_tag="DLJ67_RS29115"
     CDS             complement(3697..3909)
                     /locus_tag="DLJ67_RS29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3913..4398)
                     /locus_tag="DLJ67_RS29120"
     CDS             complement(3913..4398)
                     /locus_tag="DLJ67_RS29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4436..5113)
                     /locus_tag="DLJ67_RS29590"
                     /pseudo=""
     CDS             complement(<4436..5113)
                     /locus_tag="DLJ67_RS29590"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(260..432)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          379..892
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..145)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.833"
                     /coverage="99.0"
                     /evalue="1.88e-24"
     Typing          complement(3582..5086)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgccggcga tatcgacggc agtctgccgg cacgcgccgg cttctcatcg aggcaggtct
       61 gacgcatcca cgccgccagc tgcttgccgt cgcaccgctc gagcagccgc cggtgttcgt
      121 cttccgtcac ccacatcgtg agcatcttgt tgcgtttgtc tgccagagta gcctcctgat
      181 aacaccggga cgggcgggaa atgtttcccg gtcggcacgg tgttggacaa gccgcaggcg
      241 cgtcagtcgc ttttagcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
      301 gagtgtatac tggcttatta tgttggcatg agtgcggtaa tcaggaaagt gcttcatgtg
      361 acagaggaaa atgcagctgc gggcgtaagg cagaaaatgg aagttcagag gatatgacgc
      421 ctcctcgctc actcagtcgc tgccgctcct gccgttcggc tgcggcgggc gttacctgca
      481 cacggagaag agcgaaggca tatggcatga caaacggggc agaaaaagga tgcgccgctg
      541 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
      601 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg tggctccctc
      661 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
      721 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
      781 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttc tccggtaact atcatcttga
      841 gtccaacccg gaaagacacg gttcatcgcc actggcagca gccattggta acggattaga
      901 ggaagtgagt cttgaagttc tgcgccagtt aaggctaaac tgaaaggaca atttttggtg
      961 actgcgctcc ctctaagcca gttacccggt acataagttg gtagctcatg taccttccaa
     1021 aaaccaccgt tggtagcggt ggtttttatt tttcatagat tacagcctgt aataagccat
     1081 ggtcaatgga ttacttcaaa agtcgtcaat cgccttaagt agctcatgga atgatgtata
     1141 acatggaaat agataaggtt cgtgcgtgtt tttataatta gttttagcgt aaatatctag
     1201 ctttcttgcg ttatctttcg cagtaccaat cctctctctt atagcaaggt aagttagttt
     1261 actaccttta tcatagttag ttattaattt ttttagcggg gagctagaca tcaaattatc
     1321 atacgacgaa aaatctttgg ttgtgaagcc gaaatgcaac aataaccata attcaaaaca
     1381 aacacaggat attgctatat ttatcccatg cctattagct ttattccatg ccttttgatg
     1441 acatgaaata gacgaaggcg tcagatgctc ttgatcataa acaacccaaa agacgtcacc
     1501 ttctgcggaa gatgatgaat tctttttctt tatcgcctca tccaccaact gctcaggtgt
     1561 atttttcctt gttttaggta ttttaaatac acttaaagtt cttttagatc tgtctgaaat
     1621 ataagcctca aggtataatg gttcggtttt ctcaccctca cagtatatat acattacttg
     1681 tgggactttc ttattatttc catgtttttt actcatcaga aagatccccg tcaaactctt
     1741 caaaaaaacc atcagacaat tctttgacgt caatatccgg cattaccaag gatatatcac
     1801 cagatataaa tgaggctacc tttaagtagt ttatgtcggg aaccccacca aacctaccct
     1861 catcatacca agctgcgtaa ggtgttgttg tttttatctt tttcttatca aaatcatcta
     1921 agctataaag agacgacgca cctttatttt tttcacaaaa ccatatctga tctcttctca
     1981 ttttctctgg agccattaac tgcatattat gggttgagaa tacaagttgt gagccttttt
     2041 tattgacatc aggatcatta aataacttaa caatcaagtc agcaatatgc gggtgcaagc
     2101 tattatctaa ctcatcaaca attacaacca tactatcatt aaacgcagat ctaattagag
     2161 gtatgatttc aaataatttt tgggtgcctt cagactctct ccccataggg aaagaaacac
     2221 tccccccttc atcagtagga tgagagaaca tataacgata tttataatct tcttcaatta
     2281 tctcctttat ttcttgaggg atttcctcat ccatttttat tggtatttcc gcctcaacct
     2341 cccgtacaga tataccggaa acaccagtgt caaccatgca taaaaccttg gccgtttcat
     2401 ttacgacgtc ctccctctcc ccaaaagaag atattcttat cttttcattt aaacctatat
     2461 gtctaattcc ctttctaaaa aagttataag cttctttaat tatgtctggt gatgcggcat
     2521 tattacctgc tttcgctaag tatgaattat tagggaagaa tggtattttt ttaatacctc
     2581 ctttataatg cccaccaaac ttgattgttt cccaggtgtc gctctcatct ctagtaaata
     2641 aatttgcttt aaccctagat ggataatagt ctaatgattc aaataaaatc cgatttttaa
     2701 taaaagcaac ttcataactg aaccgattcc cctcaacaga ataaaattca gcctcaaact
     2761 ttacaggagc ctcattcgta gccgaagaaa gagcatatgg ctcatagcag ggaatccgtt
     2821 taccttcttt caaatcaccg ctttctgttg cgataaattt tatagcttca aaagccatga
     2881 gaacgttaga tttaccagac gcattagcac cataaatccc agcactcatg agcgttccaa
     2941 cattcattcc atcattttta tagatatgag ttgttaaatg gttactagaa ccggatgcgc
     3001 tcaagtttaa tgtcgatttt tccttaaccg acctaaaatt ttcaatagtc aaataaacaa
     3061 acatgcatac ctcctaacgg ggatttttcg ccaaaaaagc gaaaaatcaa tcataggaaa
     3121 gatatcctat atgttttaat tttggaacaa catcttcctt aaaaaacgtt gtgctattta
     3181 taattacacg ctatttacca taacatctgt tgcccgcacc gctccggaca ggttcgccac
     3241 aaacggggcg gcagtcaggt aaaaccggca acaaaccacc cgatacaccg ccccgttcgt
     3301 gacagataaa ttttacccgg ctgcatggtc atgcagccag atgcatacgg tctgatttca
     3361 ggccagaatc cggatgaacg ccttaaaaac aggaaaatgc ttctccagcc agtcccagat
     3421 ctccagcgcc atgtacaggc aattcagcac cgtctcaatc acgttaaaca gtctcataat
     3481 ccctccgttg tatacgctgt gatggaaaca tcacgaccgt atttagcggg aaatttatga
     3541 gcccttcttc ttggggtttg tcactggatg cgcgcagtca gcccagcgtc ggaccgtcat
     3601 agctccgctg ccgggaacgg gtttccgcca gctctttttc atgctgtttc tgctcgtatg
     3661 cccgttcggc ggccaccgcc atttcatgcc gctgcactac tggctcaaac gctgtaactg
     3721 cctgctcaag ttcgccacct gctgactcaa gcgcgtgacg cgctcgttca gcgccgcgtt
     3781 ctcccgttgc ataagaccag acatcttctg ccattccgcg aaggcgctct cccactcgtc
     3841 cagcctttgc gagtagtcct gatgtagctg ctctaatgcg gtcagcaact gtttttccag
     3901 ttccgtcatg ggctatttcc tctccagtac catccagttc ccttcctccc cgtaagctcc
     3961 cgctttcggg ttcactttga cgcaggacag tttcttctcc ccgcagagcg acagctgcac
     4021 gccgtggttc ttctccgaca gcatggcctg cgtccgctcc tgcgcccgga tagtctggta
     4081 attcccggtg atctgctgcc cctgccacca cagaatgctc ccgctcgtgg cgatcagcag
     4141 taccgagacc agggtgatgg tcagccacgt ccggccgacc atacgcagca cgctcagccg
     4201 gttcgactgc atcgccgcgg tcataccctg ctcgtgggcg ctgatggcgt cgctgattct
     4261 cttcgcgctt gcgctcagtt ccgcttttac agactgctcg tgctcgctga atgcggcttt
     4321 cagcatctcg ccggtactct gctgctgcgc tttcgattgc tgctccaagt ccttcgccag
     4381 cgctaaaaga ctgctcataa atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     4441 cggcaatgct gatactgttt tttgtggtgc ggaccacctc aaaacctgcc gctgtgagcg
     4501 cccctgtgac gtcctcgcgc gttttgagct ccccggatga ggctaaggcc agcaacccac
     4561 gtgtaatcgc ctgagcggct tccagcttcg tttccggcag tgcggacggc gtgaccagta
     4621 accgtcggtt ctccggggcg tttggatcat gcagccccag cctgccgttc accacggtct
     4681 gccaggcatc gatgcgcggc cggtcggcgc gatcgtagta cggctgcagg cgtttcccgg
     4741 tcagcagttc agtgttgggg atcaggaaat tcagctccag ccgccccttg tcggtgtgct
     4801 ccacccacag aatgctgtac tggtctttat cgagcccggg catcagaacc cgctcaaagc
     4861 tggccatgat ctgctcccgc tgcccgggcg gcagttcagc ctccgcgaac gacagcacgc
     4921 cggaggtgta ctttttcgca tacggcgagg catcgataag ctcccggact tcctccggtt
     4981 ttccctgcag cacggtcgcc ccctcgcgct gccggtcttt tcccagcagg taatccaccg
     5041 gaccggcacc accaccgcgc ccccgggggt gaaatttaac gatcatcatc cccccctttt
     5101 tccagcaccg catgccgcag ccgctccagt cccgcgtcga ttgccatcag tgcggccaca
     5161 atctgcaccc ggtcatgacc ggcaccaccg gcattcactt tccgggcgat ctggttcagg
     5221 ttgttcccca taccggcaag ctggcgcagc a
//
