LOCUS       NZ_CP029589             5251 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain DA33141 plasmid pDA33141-5, complete
            sequence.
ACCESSION   NZ_CP029589
VERSION     NZ_CP029589.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09245752
            Assembly: GCF_003181095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     High prevalence of unstable heteroresistance caused by gene
            amplification in clinical bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2018) Department of Medical Biochemistry and
            Microbiology, Uppsala University, Husargatan 3, Uppsala 75442,
            Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics workbench v. 11.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,308
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 66 of 134
            Pseudo Genes (incomplete)         :: 64 of 134
            Pseudo Genes (internal stop)      :: 14 of 134
            Pseudo Genes (multiple problems)  :: 10 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029589.1.
            Bacteria and source DNA available from Dan I Andersson.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DA33141"
                     /serotype="ST16"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pDA33141-5"
                     /geo_loc_name="Sweden"
     gene            complement(576..896)
                     /locus_tag="DLJ66_RS27920"
                     /old_locus_tag="DLJ66_27920"
     CDS             complement(576..896)
                     /locus_tag="DLJ66_RS27920"
                     /old_locus_tag="DLJ66_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1533..1694
                     /locus_tag="DLJ66_RS27930"
                     /old_locus_tag="DLJ66_27930"
     CDS             1533..1694
                     /locus_tag="DLJ66_RS27930"
                     /old_locus_tag="DLJ66_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(1847..2467)
                     /locus_tag="DLJ66_RS27935"
                     /old_locus_tag="DLJ66_27935"
     CDS             complement(1847..2467)
                     /locus_tag="DLJ66_RS27935"
                     /old_locus_tag="DLJ66_27935"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(2460..3815)
                     /locus_tag="DLJ66_RS27940"
                     /old_locus_tag="DLJ66_27940"
     CDS             complement(2460..3815)
                     /locus_tag="DLJ66_RS27940"
                     /old_locus_tag="DLJ66_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(4448..4660)
                     /locus_tag="DLJ66_RS29080"
     CDS             complement(4448..4660)
                     /locus_tag="DLJ66_RS29080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4664..5149)
                     /locus_tag="DLJ66_RS29085"
     CDS             complement(4664..5149)
                     /locus_tag="DLJ66_RS29085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     Typing          complement(1011..1183)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          1130..1643
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6..613)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.626"
                     /coverage="94.0"
                     /evalue="7.4e-115"
     Typing          complement(4333..5249)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cccctgtgac gtcctcgcgc gttttgagct ccccggatga ggctaaggcc agcaacccac
       61 gtgtaatcgc ctgagcggct tccagcttcg tttccggcag tgcggacggc gtgaccagta
      121 accgtcggtt ctccggggcg tttggatcat gcagccccag cctgccgttc accacggtct
      181 gccaggcatc gatgcgcggc cggtcggcgc gatcgtagta cggctgcagg cgtttcccgg
      241 tcagcagttc agtgttgggg atcaggaaat tcagctccag ccgccccttg tcggtgtgct
      301 ccacccacag aatgctgtac tggtctttat cgagcccggg catcagaacc cgctcaaagc
      361 tggccatgat ctgctcccgc tgcccgggcg gcagttcagc ctccgcgaac gacagcacgc
      421 cggaggtgta ctttttcgca tacggcgagg catcgataag ctcccggact tcctccggtt
      481 ttccctgcag cacggtcgcc ccctcgcgct gccggtcttt tcccagcagg taatccaccg
      541 gaccggcacc accaccgcgc ccccgggggt gaaatttaac gatcatcatc cccccctttt
      601 tccagcaccg catgccgcag ccgctccagt cccgcgtcga ttgccatcag tgcggccaca
      661 atctgcaccc ggtcatgacc ggcaccaccg gcattcactt tccgggcgat ctggttcagg
      721 ttgttcccca taccggcaag ctggcgcagc agcgccggcg atatcgacgg cagtctgccg
      781 gcacgcgccg gcttctcatc gaggcaggtc tgacgcatcc acgccgccag ctgcttgccg
      841 tcgcaccgct cgagcagccg ccggtgttcg tcttccgtca cccacatcgt gagcatcttg
      901 ttgcgtttgt ctgccagagt agcctcctga taacaccggg acgggcggga aatgtttccc
      961 ggtcggcacg gtgttggaca agccgcaggc gcgtcagtcg cttttagcgg gtttcggggc
     1021 gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttatt atgttggcat
     1081 gagtgcggta atcaggaaag tgcttcatgt gacagaggaa aatgcagctg cgggcgtaag
     1141 gcagaaaatg gaagttcaga ggatatgacg cctcctcgct cactcagtcg ctgccgctcc
     1201 tgccgttcgg ctgcggcggg cgttacctgc acacggagaa gagcgaaggc atatggcatg
     1261 acaaacgggg cagaaaaagg atgcgccgct gccgtttttc cataggctcc gcccccctga
     1321 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     1381 ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt
     1441 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1501 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     1561 gctgcgcctt ctccggtaac tatcatcttg agtccaaccc ggaaagacac ggttcatcgc
     1621 cactggcagc agccattggt aacggattag aggaagtgag tcttgaagtt ctgcgccagt
     1681 taaggctaaa ctgaaaggac aatttttggt gactgcgctc cctctaagcc agttacccgg
     1741 tacataagtt ggtagctcat gtaccttcca aaaaccaccg ttggtagcgg tggtttttat
     1801 ttttcataga ttacagcctg taataagcca tggtcaatgg attacttcaa aagtcgtcaa
     1861 tcgccttaag tagctcatgg aatgatgtat aacatggaaa tagataaggt tcgtgcgtgt
     1921 ttttataatt agttttagcg taaatatcta gctttcttgc gttatctttc gcagtaccaa
     1981 tcctctctct tatagcaagg taagttagtt tactaccttt atcatagtta gttattaatt
     2041 tttttagcgg ggagctagac atcaaattat catacgacga aaaatctttg gttgtgaagc
     2101 cgaaatgcaa caataaccat aattcaaaac aaacacagga tattgctata tttatcccat
     2161 gcctattagc tttattccat gccttttgat gacatgaaat agacgaaggc gtcagatgct
     2221 cttgatcata aacaacccaa aagacgtcac cttctgcgga agatgatgaa ttctttttct
     2281 ttatcgcctc atccaccaac tgctcaggtg tatttttcct tgttttaggt attttaaata
     2341 cacttaaagt tcttttagat ctgtctgaaa tataagcctc aaggtataat ggttcggttt
     2401 tctcaccctc acagtatata tacattactt gtgggacttt cttattattt ccatgttttt
     2461 tactcatcag aaagatcccc gtcaaactct tcaaaaaaac catcagacaa ttctttgacg
     2521 tcaatatccg gcattaccaa ggatatatca ccagatataa atgaggctac ctttaagtag
     2581 tttatgtcgg gaaccccacc aaacctaccc tcatcatacc aagctgcgta aggtgttgtt
     2641 gtttttatct ttttcttatc aaaatcatct aagctataaa gagacgacgc acctttattt
     2701 ttttcacaaa accatatctg atctcttctc attttctctg gagccattaa ctgcatatta
     2761 tgggttgaga atacaagttg tgagcctttt ttattgacat caggatcatt aaataactta
     2821 acaatcaagt cagcaatatg cgggtgcaag ctattatcta actcatcaac aattacaacc
     2881 atactatcat taaacgcaga tctaattaga ggtatgattt caaataattt ttgggtgcct
     2941 tcagactctc tccccatagg gaaagaaaca ctcccccctt catcagtagg atgagagaac
     3001 atataacgat atttataatc ttcttcaatt atctccttta tttcttgagg gatttcctca
     3061 tccattttta ttggtatttc cgcctcaacc tcccgtacag atataccgga aacaccagtg
     3121 tcaaccatgc ataaaacctt ggccgtttca tttacgacgt cctccctctc cccaaaagaa
     3181 gatattctta tcttttcatt taaacctata tgtctaattc cctttctaaa aaagttataa
     3241 gcttctttaa ttatgtctgg tgatgcggca ttattacctg ctttcgctaa gtatgaatta
     3301 ttagggaaga atggtatttt tttaatacct cctttataat gcccaccaaa cttgattgtt
     3361 tcccaggtgt cgctctcatc tctagtaaat aaatttgctt taaccctaga tggataatag
     3421 tctaatgatt caaataaaat ccgattttta ataaaagcaa cttcataact gaaccgattc
     3481 ccctcaacag aataaaattc agcctcaaac tttacaggag cctcattcgt agccgaagaa
     3541 agagcatatg gctcatagca gggaatccgt ttaccttctt tcaaatcacc gctttctgtt
     3601 gcgataaatt ttatagcttc aaaagccatg agaacgttag atttaccaga cgcattagca
     3661 ccataaatcc cagcactcat gagcgttcca acattcattc catcattttt atagatatga
     3721 gttgttaaat ggttactaga accggatgcg ctcaagttta atgtcgattt ttccttaacc
     3781 gacctaaaat tttcaatagt caaataaaca aacatgcata cctcctaacg gggatttttc
     3841 gccaaaaaag cgaaaaatca atcataggaa agatatccta tatgttttaa ttttggaaca
     3901 acatcttcct taaaaaacgt tgtgctattt ataattacac gctatttacc ataacatctg
     3961 ttgcccgcac cgctccggac aggttcgcca caaacggggc ggcagtcagg taaaaccggc
     4021 aacaaaccac ccgatacacc gccccgttcg tgacagataa attttacccg gctgcatggt
     4081 catgcagcca gatgcatacg gtctgatttc aggccagaat ccggatgaac gccttaaaaa
     4141 caggaaaatg cttctccagc cagtcccaga tctccagcgc catgtacagg caattcagca
     4201 ccgtctcaat cacgttaaac agtctcataa tccctccgtt gtatacgctg tgatggaaac
     4261 atcacgaccg tatttagcgg gaaatttatg agcccttctt cttggggttt gtcactggat
     4321 gcgcgcagtc agcccagcgt cggaccgtca tagctccgct gccgggaacg ggtttccgcc
     4381 agctcttttt catgctgttt ctgctcgtat gcccgttcgg cggccaccgc catttcatgc
     4441 cgctgcacta ctggctcaaa cgctgtaact gcctgctcaa gttcgccacc tgctgactca
     4501 agcgcgtgac gcgctcgttc agcgccgcgt tctcccgttg cataagacca gacatcttct
     4561 gccattccgc gaaggcgctc tcccactcgt ccagcctttg cgagtagtcc tgatgtagct
     4621 gctctaatgc ggtcagcaac tgtttttcca gttccgtcat gggctatttc ctctccagta
     4681 ccatccagtt cccttcctcc ccgtaagctc ccgctttcgg gttcactttg acgcaggaca
     4741 gtttcttctc cccgcagagc gacagctgca cgccgtggtt cttctccgac agcatggcct
     4801 gcgtccgctc ctgcgcccgg atagtctggt aattcccggt gatctgctgc ccctgccacc
     4861 acagaatgct cccgctcgtg gcgatcagca gtaccgagac cagggtgatg gtcagccacg
     4921 tccggccgac catacgcagc acgctcagcc ggttcgactg catcgccgcg gtcataccct
     4981 gctcgtgggc gctgatggcg tcgctgattc tcttcgcgct tgcgctcagt tccgctttta
     5041 cagactgctc gtgctcgctg aatgcggctt tcagcatctc gccggtactc tgctgctgcg
     5101 ctttcgattg ctgctccaag tccttcgcca gcgctaaaag actgctcata aatggctccc
     5161 ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc tgatactgtt ttttgtggtg
     5221 cggaccacct caaaacctgc cgctgtgagc g
//
