LOCUS       NZ_CP029573             8963 bp    DNA     circular CON 04-JAN-2024
DEFINITION  Acinetobacter baumannii strain DA33098 plasmid pDA33098-9, complete
            sequence.
ACCESSION   NZ_CP029573
VERSION     NZ_CP029573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09241862
            Assembly: GCF_003181015.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     High prevalence of unstable heteroresistance caused by gene
            amplification in clinical bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Nicoloff,H., Hjort,K. and Andersson,D.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2018) Department of Medical Biochemistry and
            Microbiology, Uppsala University, Husargatan 3, Uppsala 75442,
            Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics workbench v. 11.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 17:52:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,109
            CDSs (total)                      :: 4,014
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 29 of 92
            Pseudo Genes (incomplete)         :: 55 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 7 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029573.1.
            Bacteria and source DNA available from Dan I. Andersson.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="DA33098"
                     /serotype="ST350"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pDA33098-9"
                     /geo_loc_name="Spain"
     gene            complement(join(8959..8963,1..823))
                     /locus_tag="DLD53_RS20660"
                     /old_locus_tag="DLD53_20655"
     CDS             complement(join(8959..8963,1..823))
                     /locus_tag="DLD53_RS20660"
                     /old_locus_tag="DLD53_20655"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /protein_id="WP_012754353.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MGVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
     gene            1132..1419
                     /locus_tag="DLD53_RS20665"
                     /old_locus_tag="DLD53_20660"
     CDS             1132..1419
                     /locus_tag="DLD53_RS20665"
                     /old_locus_tag="DLD53_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            1406..1720
                     /locus_tag="DLD53_RS20670"
                     /old_locus_tag="DLD53_20665"
     CDS             1406..1720
                     /locus_tag="DLD53_RS20670"
                     /old_locus_tag="DLD53_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            1848..4259
                     /gene="znuD2"
                     /locus_tag="DLD53_RS20675"
                     /old_locus_tag="DLD53_20670"
     CDS             1848..4259
                     /gene="znuD2"
                     /locus_tag="DLD53_RS20675"
                     /old_locus_tag="DLD53_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_012754356.1"
                     /translation="MLNKSKLFLALITLGASKIVLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            4564..5025
                     /locus_tag="DLD53_RS20680"
                     /old_locus_tag="DLD53_20675"
     CDS             4564..5025
                     /locus_tag="DLD53_RS20680"
                     /old_locus_tag="DLD53_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(5348..5521)
                     /locus_tag="DLD53_RS20935"
     CDS             complement(5348..5521)
                     /locus_tag="DLD53_RS20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(5521..5892)
                     /locus_tag="DLD53_RS20685"
                     /old_locus_tag="DLD53_20680"
     CDS             complement(5521..5892)
                     /locus_tag="DLD53_RS20685"
                     /old_locus_tag="DLD53_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(6163..6417)
                     /locus_tag="DLD53_RS21245"
                     /old_locus_tag="DLD53_20685"
     CDS             complement(6163..6417)
                     /locus_tag="DLD53_RS21245"
                     /old_locus_tag="DLD53_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            7103..8053
                     /gene="repM"
                     /locus_tag="DLD53_RS20700"
                     /old_locus_tag="DLD53_20695"
     CDS             7103..8053
                     /gene="repM"
                     /locus_tag="DLD53_RS20700"
                     /old_locus_tag="DLD53_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208776.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            8046..8621
                     /locus_tag="DLD53_RS20705"
                     /old_locus_tag="DLD53_20700"
     CDS             8046..8621
                     /locus_tag="DLD53_RS20705"
                     /old_locus_tag="DLD53_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012754352.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAMPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            8633..8776
                     /locus_tag="DLD53_RS20940"
     CDS             8633..8776
                     /locus_tag="DLD53_RS20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     ARG             complement(3..823)
                     /name="blaOXA"
                     /gene="blaOXA"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="99.64"
     ARG             complement(3..823)
                     /name="OXA-24"
                     /gene="OXA-24"
                     /gene_name="OXA beta-lactamase; OXA-24-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; carbapenem; penam"
                     /identity="100.0"
                     /coverage="99.64"
     Typing          7104..8053
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.264"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattccaag attttctagc gacttataag taatttcatt acgaatagaa ccagacattc
       61 cttctttcat ttctaagttg agcgaaaagg ggattttttt tccattagct tgctccaccc
      121 aaccagtcaa ccaacctacc tgtggagtaa cacccattcc ccatccactt tttgcataaa
      181 tcttactacc atttacttct ttaattagaa gcattttttt aacttcttct tgagtttcta
      241 atttaaaagg taatcggtta tgtgcaaggt catcggcaaa attaacttct tgtactggtg
      301 taattttaag ggggccaact aaccaaaaat tatcgacctg tgttccaata tttgtatttc
      361 caaaattaac ccgctttact tctttctgca ttagctctag gccagtccgt cttgcaagct
      421 cttgatatac tggaactgct gacaatgcca ttgcctcacc taaagtcata tctttctccc
      481 acataggata agttcttttt ttaccatccc atttgaaaat ctcatttgtt gttgctttat
      541 gattttctag cccgattaaa gcatttagca tcttaaatgt tgatgcaggg acatattctt
      601 tatttgctcg tgcaagagca ttaccatagg tgctaagatt tttaccctct ttaataataa
      661 ttacaccctg tgtttgagct tcatcaaaat agcttttaat agctttttca tgttgctgag
      721 aagaaatatg aaaattatct tcagatttag ttttaataga tgaacatgca ctgagagaaa
      781 ctagaataga aatgctgaat ataggaagta taaatttttt cattacaaat tcatgttagg
      841 ggaaattttg gggcttagag tataatattt ttattaaaaa agttaaaact cttttaattt
      901 aacataatgg cgattatacg aagtacattt gtatattaat ttaaatatca actacttaat
      961 aaaaatagaa aaatccaaaa atgcccaaaa agccgacttt gaaacaagtc ggctttttta
     1021 tgagcagttt tgatacttct tgccaataaa tttgactaaa aattacgctt ttcagcttga
     1081 gtgatacaaa tatgtgatac attattgtat atacaaattt gaatgttttt tatggaacag
     1141 tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga tatctctttc
     1201 gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga cagacatacc
     1261 aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat gttattggtg
     1321 gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag tgcaaggcaa
     1381 gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca aagagctgaa
     1441 tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta aaggcacggt
     1501 tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga gcaatgctgt
     1561 acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt tagatgctga
     1621 tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga atgaaatttt
     1681 acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc ttaggctccc
     1741 atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt agattttata
     1801 aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg ctaaataaaa
     1861 gtaaactatt tttagcttta attactttag gggcctcgaa aattgtattg gcagctgaag
     1921 ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag ttagggtctg
     1981 agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt aaacttaaac
     2041 agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat tctaatcaat
     2101 atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt attaaagttt
     2161 tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac gctgtcacag
     2221 ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc cttttgtata
     2281 gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca acccaaatgc
     2341 ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt aatgagaagt
     2401 tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg gcagaagggt
     2461 tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct tataattctt
     2521 tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta ccagaaagct
     2581 gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat acttatttag
     2641 gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc catttatatg
     2701 aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta aaaaattatc
     2761 ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat ccacgtcctg
     2821 attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct ccttatattg
     2881 atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt accggtattg
     2941 acaaaattcg tacctcattg agttatatag attattttca caatgaactc gagggagata
     3001 agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta tcacatcagc
     3061 cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat aatagtgctt
     3121 tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag cagctattaa
     3181 atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac tggaacgact
     3241 ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat tatgatatag
     3301 aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct tatgtagaaa
     3361 aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt caacctaaca
     3421 aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca aattatatct
     3481 tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg tatactcatg
     3541 gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga aaagagaaat
     3601 ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat caaatcagta
     3661 cttactatta tgattttgat aactatatat atttacagac actcaatgaa gttttaggaa
     3721 ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag tctgctgcta
     3781 atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat cacggctctc
     3841 tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta attgcatacg
     3901 atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact cctagattac
     3961 ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt aaaggtgaaa
     4021 tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag gttacatcag
     4081 gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac acagaatatg
     4141 atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat gaaacatttt
     4201 tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt aatttctaga
     4261 taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa aatgattaga
     4321 ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc gatttaggtt
     4381 tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc agccttttca
     4441 gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc aatttttttg
     4501 atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt taataggtga
     4561 aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacaaaaaaa aattatttca
     4621 ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc atctgaagat
     4681 ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa attaagttat
     4741 gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct acttgcttta
     4801 ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc aataagtaat
     4861 acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa agttattcca
     4921 gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga tttaaacgtg
     4981 ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc ctttagatat
     5041 aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca taggattgta
     5101 aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag tacaaatcaa
     5161 tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta cttataacta
     5221 ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgag caagcatcag
     5281 ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct cttatgcgaa
     5341 gcccaggtta atcctgggct ttttgttcaa tctcatcatt ggccgagaac atacttggat
     5401 tcgtaagcct gatattttcc tgacaacttc cacacagcat caatcctgca aaaagttgta
     5461 cattttgtct attttcacaa agcgtacatg gctttgaaag ctgaataata ttatctgaca
     5521 ctactttacc ctcttatagt tatcatctgc atatgaataa cctttagctt gcatcattcg
     5581 aacctcatct ttagaagccc catttttcat taatgtccat aacatcatga aatagagtcc
     5641 agaaaaatct tcatagtatt tttcagaaaa attattatct tcctcaaaat catctattcg
     5701 cttttctaat cgattcacca gatcttcaat acgatctgcc tgctgcttaa cagcaataga
     5761 ttgaaaatca ttagtcaaaa ttttactgaa caatttactg gttctgattc cttctgaatt
     5821 agccaattga tcgtagaatt ctgctacttg tggatctagt ctgactgcat aagttttgac
     5881 tttttcgccc ataaattaca tctcttataa aaattagagt ttgtacaaga ttaaatcaag
     5941 ataattcaat aatttaaatt aaaataataa gtaaattacg tacaaatcta tttgtacaaa
     6001 attaatctat tctaaaacaa aaacttattt aaatataatg ctttttttat gtacaactat
     6061 gagtacatga cttttattaa atttatcaat actttatcta aaaaaagtat ggttttcatg
     6121 tacaagcatg tgcacagcat aaaaaacata taattcaact acttattcta aaaaaagacc
     6181 atttttctgt acacgtttct gagaccctaa aaaatcgctg aaatccttat cctgtaaggc
     6241 tttgaggggt ttcatcgtta gatgcgtggt gtgtgacaaa aaaatcccgg catgtgccgg
     6301 atcttggatt ggaaaattgg ctaaagtgac gtagggccgg tgcttggttt tgcatggaaa
     6361 aaagtattta tttccttgtt tagaaaaacg taaaaaatca attttttgtc cattcatcca
     6421 ggttaaaaat ttcaacccaa aactttaatt atgaaaagct tcacagaaag cgttcaaatg
     6481 cgatttaaga gcctttactt aaaaaacata gatctcatag tgaaaaacag aaaacagccc
     6541 aaaaaacgca aatgagaacg aaataaagag agattttaac tttagataga tgcatagagc
     6601 gagtgtctac gagcgaacta tcaaaatttg cgcctagacc ctctgaaaaa catttttttg
     6661 tcctctttag cctaagaaag cgtaagtttc atacagaaat ttgctcctgg atcgagcgta
     6721 gcgagtaaaa aagttttatg agcgaagcga attccgagtt gcttttgctt tttcttaaag
     6781 tcacgcaagt attaaccaaa aaattgcccc aacgaactga gcgaaagcga agttcaatag
     6841 agtttgagcg aagcgaaaac caagggcaat ttttcattcc ctgggctttt aattattttt
     6901 aatcttttaa atgcttttag acatgctgaa agccttaatt tacaacactt tcaaaggcta
     6961 tatgacacca tttaccttgc aatatgacac cgtttacctt gcaatatgac accgtttacc
     7021 ttgcaatatg acaccgttta ccttgcttat agcactaaaa ttaattattg tgtcatagca
     7081 taataaataa tactggtgct tcatgagaga attagttgta aaagacaatg ccttaattaa
     7141 tgcaagctat aacttagatt tagtagaaca acgtttaatt ttattggcca ttgttgaggc
     7201 aagagaaagc gggaaaggta ttaatgctaa tgatcccctt gaagtacatg cagaaggcta
     7261 tatcaatcaa tttggcgtac atcgcaatac ggcttatcaa gcattaaaag atgcctgtaa
     7321 tgatttattt gcaagacaat ttagctatca aaaaataaat gaacgaggga atattgagaa
     7381 ctatagatcc cgttgggtta gtgaaattgg atatgtagat aatgaagcag tggttaaact
     7441 tatctttgcc ccagccatag ttcccttaat tacacgctta gaagagcatt tcactaaata
     7501 cgaattgcag caagttagta atctcagcag tgcttatgct gttcgcttat atgaattatt
     7561 aattgcttgg agaagtactg gctctactcc tattatagag gtaagtgatt tccgtcaaag
     7621 aattggcgta ctcgatacag agtacaagcg tatggaacgc tttaaaacta gtgtacttga
     7681 gcttgctatt aaacaaatta acgaacatac agatatcact gtgaagtatg agcaacacaa
     7741 aagaggtcga tcaatttcag gattctcttt tacttttaaa cagaaaaaga aggacaaccc
     7801 accgatagaa agagatccga atacgttaga tctctttaca aagatgactg atgcacaacg
     7861 ccatctgttt gcaaataaac tttctgaact tcctgaaatg ggtcgttatt cccagggaac
     7921 tgaaagctat ccgcaatttg ctattcgtat tgctgagatg ctgcaagacc ctgaccgaat
     7981 aaaagaacta tacccatacc taaaaaaagt gggatatatg ccatcaaata aaaaggacac
     8041 cgtaaatggc taagttatca ctaagtgaag tatctaaaaa attccatgtg gatagatcaa
     8101 ccatttacag agctgtacgt aatggacgtt tatcacgctc cagtgatgga caattcgatc
     8161 tagcagaggt catccgatgc tttggagaac ctgagcaaac accccaaaaa attgaatcat
     8221 ctaagcaaga aggtgatgaa tctacaaaaa aacttattgc ccatttagaa aatgaagtca
     8281 aaaaatacca agaacgtgaa gaacggttaa tgcaacaaat tgaccgtatg caaacactca
     8341 ttgagctgaa aagtgttgca cctgccacag catcaccaca acaagatgct acggcatgcg
     8401 acactgacat gcaacagcat gcgacaccac aacaagacaa tgataataaa aagaatcatg
     8461 aattaaatat tgcagaaaat gtggcaatgc cacagcaaga aaatacggca taccacaccc
     8521 aaacgctaca gcatgccacg ttgcaaaatg tggcagtgcc acaacacaaa aaacgtggct
     8581 tatttggccg tgtgctgaat gctgtttttg ataatgacta agggagggga tcatgccgaa
     8641 actgaaagat attgccctgg gaattattgt agctccgctg ctgatcccga tcatgctgat
     8701 tgcatcgtac caggataaaa aggcgctgaa gaaagagctg gatgaatgcc aaaaagaaaa
     8761 agaccaggca tcctgattat tcaattgagc taatcaaaag tgtggaacat tgatgaaata
     8821 atagcgtgga acgtgaaaag tcataaaaaa agcccgaaca gggatgctcg ggctataaac
     8881 tagaaactac agcgttgaat tataaggata attatacagc atttcgtata acgtgtatta
     8941 tgttaatttt agaaattatt aaa
//
