LOCUS       NZ_CP029391             5017 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Acinetobacter defluvii strain WCHA30 plasmid p3_010030, complete
            sequence.
ACCESSION   NZ_CP029391
VERSION     NZ_CP029391.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356262
            Assembly: GCF_001704615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter defluvii
  ORGANISM  Acinetobacter defluvii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Hu,Y., Qin,J., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter defluvii strain WCHAD010030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Hu,Y., Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 5017)
  AUTHORS   Hu,Y., Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 5017)
  AUTHORS   Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:24:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,531
            CDSs (with protein)               :: 3,531
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 34 of 85
            Pseudo Genes (incomplete)         :: 54 of 85
            Pseudo Genes (internal stop)      :: 27 of 85
            Pseudo Genes (multiple problems)  :: 24 of 85
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029391.2.
            On Aug 26, 2019 this sequence version replaced NZ_CP029391.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Acinetobacter defluvii"
                     /mol_type="genomic DNA"
                     /strain="WCHA30"
                     /isolation_source="sewage"
                     /type_material="type strain of Acinetobacter defluvii"
                     /db_xref="taxon:1871111"
                     /plasmid="p3_010030"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2015-10-31"
     gene            join(4469..5017,1..9)
                     /locus_tag="DJ533_RS00520"
                     /old_locus_tag="DJ533_00545"
     CDS             join(4469..5017,1..9)
                     /locus_tag="DJ533_RS00520"
                     /old_locus_tag="DJ533_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_005146114.1"
                     /translation="MKEIRQQKKQPEVIRKRILEQATILVSQKGISGVSIQNIATAVGI
                     TKGGVFHHFPNKKNLIEEMFNQIILDLDQTVEYLIKRDPTEYGKFTRAYIHSSFIHQID
                     GVVSAWSALSMTMITEPTFNKIWMEWLKNRLSQHAETDSHPDLELLRLAADGLWLQSIT
                     GVIDDEQCSKLEDDLLNRTYLT"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            complement(137..634)
                     /locus_tag="DJ533_RS00525"
                     /old_locus_tag="DJ533_00540"
     CDS             complement(137..634)
                     /locus_tag="DJ533_RS00525"
                     /old_locus_tag="DJ533_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856450.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDKKRLEQFYNQVASGVLD
                     KAEKSLENTNKKHTGALNNSIQALNEATNRLNLKLIFIFASTFVAVMMVFILAIFLYIP
                     SKDEIDERRADMAVLKKYPLQIRESDGETLVRIMRKNCYSFEPSSVKEKTYDWCQIDPK
                     KY"
                     /name="hypothetical protein"
     gene            complement(631..1602)
                     /gene="mobQ"
                     /locus_tag="DJ533_RS00530"
                     /old_locus_tag="DJ533_00535"
     CDS             complement(631..1602)
                     /gene="mobQ"
                     /locus_tag="DJ533_RS00530"
                     /old_locus_tag="DJ533_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_065995591.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRAGEKLEDERTGLTHDFTRK
                     DGVAHAEILSNLDIEIDRGELWNLAEKTENRKDARTAREWVIALPDELDADQRKDLAKD
                     FARSLVDRYDVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDADNKLTLATKTDIELS
                     NAKRKSLGMGTTQDEIKQIRETWANLANHALERAGYREKIDHRSYADQNNGLQATIHEG
                     TKVTQLRRQGIDTEISRFNDNVKQQNAQQLDQQKQQKESVLQRGLNRVDQGFDQWQKNQ
                     ETKRLELERQAEMKRQQELDKQRAEQALRKAYQKLSRGGMSL"
                     /name="mobQ"
     gene            1811..2095
                     /locus_tag="DJ533_RS00535"
                     /old_locus_tag="DJ533_00530"
     CDS             1811..2095
                     /locus_tag="DJ533_RS00535"
                     /old_locus_tag="DJ533_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004871868.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIEARERTKKKEQERKDD
                     TRRKILLGSYLIKKMQANEANKEKILAELNEYLTENRDRQLFDLPDIEA"
                     /name="hypothetical protein"
     gene            complement(2092..2388)
                     /locus_tag="DJ533_RS00540"
                     /old_locus_tag="DJ533_00525"
     CDS             complement(2092..2388)
                     /locus_tag="DJ533_RS00540"
                     /old_locus_tag="DJ533_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005265043.1"
                     /translation="MIKLNNLSTDLKHVTVEYLDIVNYEIARENICGYIFLLSRLSKNA
                     KPTEKMQMESKIQDLIYYRDHLQIEDKDNIQKVLNTLIPEYQAEQNNQTAKKN"
                     /name="hypothetical protein"
     gene            complement(2385..3314)
                     /gene="repM"
                     /locus_tag="DJ533_RS00545"
                     /old_locus_tag="DJ533_00520"
     CDS             complement(2385..3314)
                     /gene="repM"
                     /locus_tag="DJ533_RS00545"
                     /old_locus_tag="DJ533_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871874.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004871874.1"
                     /translation="MKNSLVVKDNALINASYNLELTEQRLIMLAIINARESGQGITADS
                     KLEIHASDYAKLFNVSADASYKALREAVNNLFNRQFSYTAEYKKTGKVGIVRSRWVSRI
                     FYVDDLALLEITFAPDVVPLITRLEEHFTKYEAKQVAHLTSKYATRLYELLIAWREVGK
                     VPQLELSEFRNRLGLVDSEYTAMSDFKKRVLEPSIKQINEHTDITVTYEQHKKGRIISG
                     FSFKFKQKQQPKVEAKRDPNTPDFFIKMTDAQRHLFANKMSEMPEMGKYSQGTESYQQF
                     AIRIADMLLEPEKFRELYPILEKSGFQP"
                     /name="repM"
     gene            4124..4468
                     /locus_tag="DJ533_RS00550"
                     /old_locus_tag="DJ533_00550"
     CDS             4124..4468
                     /locus_tag="DJ533_RS00550"
                     /old_locus_tag="DJ533_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146112.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="WP_005199344.1"
                     /translation="MNSVLLAYVLLGFSIVSEVIGSSFLVKSEGFTKLFPSVMVFIFFS
                     TAFYLLSQVIKTIPLGIAYAIWAGVGIVLTAVVSYFVFKQTLDNPALIGIGFIVSGVII
                     INLFSQSAGH"
                     /name="SMR family transporter"
     ARG             4125..4468
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="48.11"
                     /coverage="106.54"
     Typing          complement(2382..3314)
                     /name="rep_cluster_1219"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.906"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(635..1602)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.666"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaacataaa atctcttatg cgaaatttca gctcgatgct ttgaaagatg gctaaagcca
       61 tgtttcaaag tcgggcaatt ttgcgcttgc tgtaatttga tttgtaatga gaaaaaccaa
      121 gcaaagtacc gatcaactag tattttttag ggtcgatctg acaccagtca tacgtttttt
      181 ctttgacact actcggttca aacgagtagc aatttttcct cattattctc accaatgttt
      241 caccatcact ttctcgaatt tgtagcggat atttttttag tacagccata tctgctctac
      301 gttcatcaat ttcgtctttg cttggtatgt ataaaaagat agccaaaatg aacaccatca
      361 tcacagcaac aaacgtgctg gcaaaaataa agataagttt taagttgagc ctgtttgtag
      421 cttcgtttaa tgcctgaata ctgttgttta atgcacctgt atgcttctta tttgtgtttt
      481 ctagcgattt ttcagccttg tctagcactc cactagcgac ttggttgtaa aactgctcta
      541 atcgcttctt atcgtctgct atggcttctc gatattcctt catggatgcc aataaaatat
      601 ataactggtc atctaaatca ttttgattac tcataatgac attccgcctc tacttagttt
      661 ttgatatgcc ttgcggagtg cttgctcggc tcgttgttta tctaattctt gctgtcgttt
      721 catttccgct tggcgttcca gttctagacg tttggtttct tggttcttct gccattgatc
      781 aaaaccttga tcgacacggt ttaaaccacg ctgtaaaacg ctctctttct gctgtttctg
      841 ttggtcaagc tgttgggcgt tctgctgttt cacattgtca ttaaaacggc tgatttcagt
      901 gtctatgcct tgtctgcgta attgggtgac tttagtgcct tcatggatgg tggcttgtag
      961 tccgttattc tgatcggcat agctgcggtg atctattttt tctcggtagc ctgctcgttc
     1021 taatgcgtga ttggctaaat ttgcccacgt ttcccgaatc tgtttaattt cgtcttgggt
     1081 tgtacccata ccaaggcttt ttcgtttggc gttgctgagt tcaatatcgg ttttggtggc
     1141 cagagtgagt ttattgtctg cgtccaattc ggcttttcgg gttgtgagca tgatatgggc
     1201 gtgatggttt ttatcattac cgcctttact cggttcatgg atggctaaat ctgcgatcac
     1261 gtcatagcga tcaactaagg acctggcaaa gtcttttgcc aagtccttac gttgatcagc
     1321 gtctaattca tcaggcaggg caattaccca ttctctagcg gttcgggcat ctttgcggtt
     1381 ctcggttttt tctgccaagt tccaaagttc gccacggtca atttcaatat ctaaattaga
     1441 cagaatttca gcatgggcga caccatcttt tctcgtaaag tcatgggtca gccctgtgcg
     1501 ttcatcttct agtttttcac ctgcacgata ggcacttgat gcaaccgcag ttcgtcctga
     1561 acttcggtta actgttttag ttgaacaatg atagattgcc atgcagtcgc cttgagatgc
     1621 ttttgctttt aaaaaattgc gtagcagttt tttggggtta aggggtgtcc ccttgcgcaa
     1681 ctttgatttg aacgaagttc aagtcgtaag tgcgcatttc atgaaaaaca cgatagcata
     1741 ttacccgctt gaatttcaat tcatagggtc ttatgcttga gtggttttct atattccgaa
     1801 attaaccgtt atgactaaat ctgctgaaaa tatcgaaaag aaaattgaag cccagttaga
     1861 gaagctgaaa cagttaaaag cacagaaaca ggctattgaa gcaagagaac gtaccaaaaa
     1921 gaaagaacag gagcgcaagg atgatactag gcgtaaaatt ttgctcggtt cttacttgat
     1981 taagaaaatg caggcaaatg aagccaataa agaaaaaata ctcgctgaac tcaacgaata
     2041 tttaacagaa aatagagatc gacagctttt tgatttgccc gacattgaag cctaattttt
     2101 cttagctgtt tggttattct gttcggcttg atactcggga atgagcgtgt ttaaaacctt
     2161 ttgaatattg tctttgtcct ctatctgcaa atgatcccga tagtaaatta agtcctgtat
     2221 tttgctttcc atctgcattt tttcggtggg tttggcattt ttggatagtc gggataatag
     2281 gaagatataa ccgcatatat tttccctcgc aatttcataa tttacgatgt cgaggtattc
     2341 aacggtcaca tgctttagat cggttgatag gttatttaat ttaatcatgg ctgaaatcct
     2401 gatttttcta aaattggata aagctctctg aatttttcag gctctaaaag catatcagcg
     2461 atacgaatag caaattgttg gtagctttct gtaccttgtg aatatttccc catttcaggc
     2521 atttctgaca ttttattcgc aaataaatgg cgttgtgcat cggtcatttt tataaaaaaa
     2581 tcaggcgtgt ttggatctct ttttgcttca acttttggct gttgtttttg cttaaatttg
     2641 aaagaaaaac ctgaaatgat ccgtcctttt ttatgctgtt catacgttac agtaatatca
     2701 gtatgctcat taatttgttt aattgatggt tcaagcacac gctttttaaa gtcactcatg
     2761 gcggtgtatt cactatcaac aagacctaat ctgtttctaa actcactaag ttctagttgt
     2821 ggaacttttc caacttcacg ccaagcaatc agcaattcat aaagcctagt ggcgtattta
     2881 ctggttaggt gagcaacttg tttagcttca tattttgtaa agtgttcttc caatcgtgtg
     2941 attaaaggca ctacatcagg agcaaaagta atttctaaca atgctaggtc atctacataa
     3001 aatatacggc ttacccagcg agatcgaaca atgccgactt tgcctgtttt tttatattcg
     3061 gctgtataac taaattgacg attaaacaga ttatttacag cttctctcaa agctttatat
     3121 gacgcatcgg cagatacatt gaatagcttg gcgtagtcac tagcatgtat ttccagttta
     3181 ctatccgcag tgattccttg tcccgattct ctagcattaa tgatagctag catgatcaaa
     3241 cgttgttccg ttagctctag gttgtaactg gcatttatta gagcattatc tttcacgact
     3301 aaactatttt tcataagaag aacttattat tatctaatta aaggacaatg taatctaata
     3361 tctttttaag gtcaatattt ataatgtctt ttaattaaga gcaaatgtcc gtttatacaa
     3421 gagcaaatgt ccgtttatac aagagcaaat gtccgtttat acaagagcaa atgtccgttt
     3481 atatagtctg taacacttac tacacaaggg tttcaacctg tctaaaagca ttaaaagctt
     3541 taaaaataat taaaagcctt tcgagagggg agtgcttaaa actgccctat gtactcgcta
     3601 aagctcgtac tctattgaag ctcgttcctc gcttcgtggg gcagtttttg gttaatcctg
     3661 tttgtgaaat ctaaaagcaa aagcattggt aatgtattcg cttcgctcat actttttgaa
     3721 aagcaaaagc tccaaagttc gcacccattc gggatgctct gtatctttcg ttatgaagca
     3781 aaagggagct tgtaatcagg gaaaaggcag gggcaaaatt ttaatgccag gtggtcgctc
     3841 gtagacactc gctcggctct tactggaatg attaaaatat ctaatcaaaa acgtggaaca
     3901 ttgtttaaat attaagcgtg gaacgtgaat aattataaaa aaaccccata cgggctagaa
     3961 tggggtatta agattagaat ataacattga tatttaagga aaataaatat cagtttcgta
     4021 taagttgtat tatgttaatt ttagaaaatc taaaataatt attgataatt tttttttgaa
     4081 aatttaacat accttccgat gggtatttta ggtatgatta cttatgaaca gtgtgttact
     4141 cgcttatgta ttacttggat tttcaatagt ttccgaagtg attggaagta gttttttagt
     4201 taaatcggaa gggtttacaa aactttttcc aagcgtgatg gtctttattt tttttagcac
     4261 ggcattttat ttattatcac aagttattaa aacaatccct ttaggtatcg catacgctat
     4321 atgggctggt gttgggattg ttctaactgc tgtagttagc tactttgttt ttaaacagac
     4381 actagataat cctgctttaa ttggaattgg ttttatcgta tctggcgtaa taattattaa
     4441 tttattttcg caatcagcag gtcattaagt gaaagaaatt cggcaacaaa agaagcaacc
     4501 agaggttatt cgaaagcgca tccttgaaca ggcaactatt cttgtttctc aaaagggtat
     4561 ttctggtgtt tctattcaaa atattgctac agcagtaggt ataacaaaag ggggggtatt
     4621 tcaccatttc ccgaataaaa aaaatttgat agaagaaatg ttcaatcaaa taattcttga
     4681 tttagatcaa acagttgaat acttgattaa acgcgatcca acagaatatg gtaaattcac
     4741 acgagcttat attcatagtt cttttatcca tcaaatagat ggggtggttt ctgcttggtc
     4801 agcgttatca atgactatga tcacagagcc tacctttaat aaaatttgga tggaatggct
     4861 aaaaaataga ttatctcaac atgcagaaac ggatagtcat cctgatttag agttacttcg
     4921 tttagcagca gatgggttgt ggctacaaag tattaccgga gtcattgatg atgagcaatg
     4981 ttcaaaatta gaagacgatc tgcttaatcg aacctat
//
