LOCUS       NZ_CP029379            11970 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain SCKP020079 plasmid
            p1_020079, complete sequence.
ACCESSION   NZ_CP029379
VERSION     NZ_CP029379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07609117
            Assembly: GCF_002852975.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain SCKP020079
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 11970)
  AUTHORS   Liu,L., Lu,Y., Lin,J., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:55:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,943
            CDSs (total)                      :: 5,822
            Genes (coding)                    :: 5,638
            CDSs (with protein)               :: 5,638
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 66 of 184
            Pseudo Genes (incomplete)         :: 126 of 184
            Pseudo Genes (internal stop)      :: 23 of 184
            Pseudo Genes (multiple problems)  :: 31 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029379.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCKP020079"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p1_020079"
                     /geo_loc_name="China: Dazhou, Sichuan"
                     /collection_date="2016-12-06"
     gene            complement(join(10379..11970,1..94))
                     /locus_tag="CLQ72_RS00005"
                     /old_locus_tag="CLQ72_00005"
     CDS             complement(join(10379..11970,1..94))
                     /locus_tag="CLQ72_RS00005"
                     /old_locus_tag="CLQ72_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(199..1686)
                     /gene="ltrA"
                     /locus_tag="CLQ72_RS00010"
                     /old_locus_tag="CLQ72_00010"
     CDS             complement(199..1686)
                     /gene="ltrA"
                     /locus_tag="CLQ72_RS00010"
                     /old_locus_tag="CLQ72_00010"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2331..2576
                     /locus_tag="CLQ72_RS00015"
                     /old_locus_tag="CLQ72_00020"
     CDS             2331..2576
                     /locus_tag="CLQ72_RS00015"
                     /old_locus_tag="CLQ72_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2850..3221
                     /gene="tolA"
                     /locus_tag="CLQ72_RS00020"
                     /old_locus_tag="CLQ72_00025"
     CDS             2850..3221
                     /gene="tolA"
                     /locus_tag="CLQ72_RS00020"
                     /old_locus_tag="CLQ72_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3218..3583
                     /locus_tag="CLQ72_RS29530"
     CDS             3218..3583
                     /locus_tag="CLQ72_RS29530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3758..4036
                     /locus_tag="CLQ72_RS00030"
                     /old_locus_tag="CLQ72_00035"
     CDS             3758..4036
                     /locus_tag="CLQ72_RS00030"
                     /old_locus_tag="CLQ72_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4024..4338
                     /locus_tag="CLQ72_RS00035"
                     /old_locus_tag="CLQ72_00040"
     CDS             4024..4338
                     /locus_tag="CLQ72_RS00035"
                     /old_locus_tag="CLQ72_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4502..4930
                     /locus_tag="CLQ72_RS00040"
                     /old_locus_tag="CLQ72_00045"
     CDS             4502..4930
                     /locus_tag="CLQ72_RS00040"
                     /old_locus_tag="CLQ72_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4956..5135
                     /locus_tag="CLQ72_RS00045"
                     /old_locus_tag="CLQ72_00050"
     CDS             4956..5135
                     /locus_tag="CLQ72_RS00045"
                     /old_locus_tag="CLQ72_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5162..5692)
                     /locus_tag="CLQ72_RS00050"
                     /old_locus_tag="CLQ72_00055"
     CDS             complement(5162..5692)
                     /locus_tag="CLQ72_RS00050"
                     /old_locus_tag="CLQ72_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5699..6430)
                     /gene="mobC"
                     /locus_tag="CLQ72_RS00055"
                     /old_locus_tag="CLQ72_00060"
     CDS             complement(5699..6430)
                     /gene="mobC"
                     /locus_tag="CLQ72_RS00055"
                     /old_locus_tag="CLQ72_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6430..8394)
                     /locus_tag="CLQ72_RS00060"
                     /old_locus_tag="CLQ72_00065"
     CDS             complement(6430..8394)
                     /locus_tag="CLQ72_RS00060"
                     /old_locus_tag="CLQ72_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9878..10027)
                     /locus_tag="CLQ72_RS00065"
                     /old_locus_tag="CLQ72_00075"
     CDS             complement(9878..10027)
                     /locus_tag="CLQ72_RS00065"
                     /old_locus_tag="CLQ72_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10112..10369)
                     /locus_tag="CLQ72_RS00070"
                     /old_locus_tag="CLQ72_00080"
     CDS             complement(10112..10369)
                     /locus_tag="CLQ72_RS00070"
                     /old_locus_tag="CLQ72_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             5379..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9197..9775
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5703..6430)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ctttaccaga cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg
       61 aattacccgg acctcgaccg tctccaccac tcataccgtt ttcctctttg acatttaaaa
      121 caataaatta aagccaacat gtcgcggtag ggatacccgt taccggatac cccccgcaca
      181 gatcccggcg tgcgcgattt acgcaccggg ctcctgcctc gggtgtctgg cggtgaaccg
      241 ctccacaggc catggatgaa gaacccgaac ccttggtagc catgcggctg ccagtttgtt
      301 tgctttcgtc caggtcgtat catccttctg gctcctgcgc ctgagcgccc ggcgccagag
      361 gtttgttacg tgtgtcctga acttctgcat ggtggggaag ttgcccggta ccgagtgata
      421 gttcaggtat ccctgaacca ctctcctgag ccattttccc tgttcgggga ttgagtaatg
      481 ccagcgcctt cgcagaccgt ctttgatggc tttcagagtt gccgtcatcc gatcccggcg
      541 ggtctttcgt atcagcatga acctgccgtt gcgatctttc ccgctgatgt gcgtgaaccc
      601 gaggaagttg aacgtttctg gtttgccttt tcccctgatg gcacggtttt cggcagcgaa
      661 gcggccgaac tccatcagac gggttttctc cgggtgaacc gtgagtccga actccctcag
      721 tctgcgctgc atggctatac ggaagcgccg ggcatcgtat cgtttgtcga acccgatgac
      781 gatgtcatcg gcgtatctga ccattaccac attgcctgtg gcatagcgac gtcgccactg
      841 atgcgcccac agatcgaaga cgtagtggag gtatatgttt gccagcagcg gtgagatgac
      901 cgcaccctgt ggggtgcctt cctccgttgc tcgccattga ccctcctccg acgtcccggc
      961 tgtgagccac ttacgtatga gcctgattac cctccggtcg ccgatccgat gctctgtgaa
     1021 cctgatcagc cattcgtggc tcaccctgtc gaagaactga ctgatgtcgg catccagtac
     1081 ccagtttacg ttagtgcgta ccagccctgt ggccagtgcg tccagtgcat cgtgctggct
     1141 tcgcccgggt ctgaacccgt atgagaaccc cataaagtcg ttttcataga ctgcgttcag
     1201 gattttcacc agcgcatact ggacgatctt gtcctccagc gaggcgatgc cgagcgggcg
     1261 ttgttttcca tccgcttttg ggatgtagtg acgcctgccg ggctgcgccc tgtagctgcc
     1321 ctgatgtagc ctccggtgca gatctgttat gttgttcttc atgtttccgg cgtagtccat
     1381 ccacctgatg ccatccactc cggcggccgc tttcctgctc agggagagga atgcggcttc
     1441 cagtgcttcg actgtcagca ggtggaacaa tgctgtaaac cgttctttct tccgctgctt
     1501 cgcagcttcc cgcacgcgtg acagcctctg tgacatgctt tcccggctct gtgtccggcg
     1561 catgtgtggc tgttccgcgt tccccttggc cccgctcctt cgctccactg actccgctcc
     1621 tttcgggttg ttcgcctgct tcgccgctac tatgagcgag tccgacttct cctctccgta
     1681 catcaccggc tatgactcct cgtcttcccg gtgcgggcca tctccgacac tggcagatgg
     1741 tcagagggga gatctcccgg ttcccgcgta gagatcgtat tgacatgcca gggtctcaga
     1801 ccccgccggg tccatgtggc actcgcagtg tcgcacccta tgatgttgcc ttccgttaac
     1861 agtacaacgt cggcacccgg taatttaata tacatttcgt ggctcaatgg ctggcctgtc
     1921 aacacccctg tcaacgcttc gccccatacc tcgcggtatg caacgcatga ctcggggacc
     1981 ttgtggattg ctggtccttc aatggtcggg gactttcacc ccttgatctc taaccggtct
     2041 cccggcgcac actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg
     2101 tgtcaatata tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt
     2161 aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca
     2221 ttcatcacac ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag
     2281 aggacctgcg gttaaactgt tcgggaaacc tgagacgccc ggaggccact atgacgcacc
     2341 tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg
     2401 ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa
     2461 gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa atgcgtcggg
     2521 agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc cggtaagggg
     2581 tatttaccat aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg tttccggcgg
     2641 ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg cccggtaaat
     2701 tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga
     2761 tacgataagc cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa tatacatgta
     2821 acatttttta ctgacaggtc atcaacccga tgcgtttaat accctttatc ctgctggcat
     2881 tgtgcagccc cttactcctg acggcctgta agcattccac cacccagccg ccgaaaattt
     2941 atactctgga taagtcatct aaaatcagta gctacgctgc ggccatcaag accgctgttg
     3001 aaatgcagat gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca
     3061 tacaacgtga tggcactctg aacagcgcca ttgctgaagg aggagatccg aaactctgta
     3121 aagcagcaat atcagccgtt acccacgcca aaattccgcc ggcaccggat gaaaatacct
     3181 ggcaaatatt caaaaacgcc cctctggatt tcagaccatg aaagctttta ctctgacagt
     3241 gctggcggcc atgttcctca ctccggtaca tgcgatacca ctaaaacccg taaccacact
     3301 gcagccaacc tacccggtac aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac
     3361 gtttgatatt ctggacgatg gacgagtgga caacgtgtcg cttaccggct gtcacctttt
     3421 ttttcgggaa acgctgacgg caatgaggca ctggctatat cccgcagggc acccggcaat
     3481 gcgacaggtt ataaattttg aattcagtct gaaaaatgac ggcttacagg atccgcgacc
     3541 agtcaccacc caaaagctca atgatgatgg ttgccgccag taacaggtcc tttaacccgc
     3601 aggctgcaat agtcctggat gatgagtgag cctgtagcag ccctgattag tggaagcgaa
     3661 gctgaaaaat gggcagcaaa agccgtattt ctggcaaata gcgccgccac ggattaaagt
     3721 agtcatgtgt aatacatatg actacaaagg ggcttttatg tcatcagcaa catccatcag
     3781 gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc accgctcagc
     3841 gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga agcgcagcca
     3901 gtacctgcgg gacggacagg ccgcatggca gcactatcag gaaacgggtc tgcaccttac
     3961 tgcggaagaa gcggaagcct ggataagtac ctggggaact gagaacgagc aggatgctcc
     4021 gccatgccac aggtaacgat ttccgcgctg gcacagcgcg atttacaacg ccttcaggac
     4081 tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat cgtccgggct
     4141 atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccgtttct gccgctggaa
     4201 tataaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta ccgccacgac
     4261 cgggaaatgg accagattgt gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg
     4321 ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg
     4381 gtttgacaag cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg gtaaaattca
     4441 gcgatacatt taccgtgaga aattacacac ctttcacacc cgataaaatc aggggtatac
     4501 agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca ttaccagaag
     4561 aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa
     4621 ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata tccctgagaa
     4681 acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac aggaacagct
     4741 tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga tgctggaaga
     4801 taaaacatcc cgggaagaag aacgccgcca gcacgatgat gagcgccgga aactccaggc
     4861 cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct ggtccggctg
     4921 gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa aaaactgccc
     4981 tcaatatggc taaattcatt aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc
     5041 tggacatgga taccccggcc gacatgtgcg aacggcttca cgaggatgcg gaaacactct
     5101 ggcaggtgat gcgggataag ctcggggaag aataacagca ccggccatgc cggtgctggc
     5161 gttatttttt gccgaacggc caccaccggc gggcgggttt gtcctccggc agttcaggca
     5221 ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt
     5281 tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat catcgcgtta atctgctccg
     5341 gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct
     5401 ttcgatagag gtaatcacac agcttcattc ctgtgggttt gtcccgttcc cattgcggat
     5461 tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc
     5521 cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc
     5581 cggtctgcca ggcgagcacc accatgtctt ccagatgctc gctggttggc ataatgcagc
     5641 gctcgccgtc tttgtcttcc acgtcctcaa taaataccat gtacagcgcc ataatccctc
     5701 actccgttgt cataaattta aagcacgcga acagcctctc ccggtccagc tccaccgttt
     5761 tgcgggcctc gccggtctgt ggattcacga tttcatccac cgccagaatc gcccgctgta
     5821 cccggacaag cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc
     5881 ggcggttgaa cagatacccg gacaccactt cctgataccg cctgcggctc ttgacggtgc
     5941 gctctacctc aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc ggcactttca
     6001 tccctttcag ggccatacgg tgcaggtacc gccagccggt ggccccccgg ccctccatgg
     6061 agaggcgcag ggactgcacg gccagccggt gcggcagctg cgccgccgac acccttgacg
     6121 gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt
     6181 gcggcgtcag cccccacacg ctgacctgac ggccgtcaac cgggtatttc gcccggacca
     6241 gcagcccttt cgccaccaga ctgtccagga agcggtaaac cggctgtacc gccgcaatcc
     6301 ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg
     6361 tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct
     6421 ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc
     6481 cggaggcagg tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcga
     6541 aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc
     6601 ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg
     6661 cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct
     6721 ggcccggcgg cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc
     6781 ggactcctgg ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt
     6841 tttcacaaaa tcagcgggca gatcctgccc gcactgaccg aagtcgccca gcgactggtg
     6901 cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtactt
     6961 cgacagcaga tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc
     7021 atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac
     7081 cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc
     7141 cacatcgata ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt
     7201 gatcagcccc ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg ccgccagcac
     7261 ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc
     7321 gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag
     7381 cagattcttc agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc
     7441 cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca
     7501 cgcgtcccct ttggggtcga agtacaccac agcgtccccg aaatcgcgta cgcagcgaat
     7561 gagcgcgttg gtggccatcg cgctcttgcc gctcccgggc aggccgagga tctgaatatt
     7621 ggtcttgcgc caggtatcac gagggaccag caccggctgg ccctgctcat ccttacccag
     7681 aaacaccgcc tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg
     7741 taccggctcc ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc gctggctgct
     7801 gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc
     7861 atcaagcagc aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa gccctccggc
     7921 acaccaggct atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcata
     7981 gaggtccggg cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc
     8041 ccccagaagg gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgtcg
     8101 ccgtgccctg accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt
     8161 ccggttatta tcgcgcttat agcgatcggc atactcccgc cgcgccttat ggctcagccc
     8221 cacaaaacgc attcggccgg cccagagttc agccagaccg aaaaacaggc cgataaccag
     8281 aaaaaaaccg gccagactgt aatactcccg gtacagccag ctgacccacg ggtaaatctg
     8341 ccgggccagc tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc
     8401 tctctccttc agacgacggg tttaccggcg tgatgcgcag cccccgctgc acaagccgcg
     8461 gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac
     8521 cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga
     8581 gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc
     8641 ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt
     8701 tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct
     8761 gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacagag ccagaaagag
     8821 gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt tcccctgaag
     8881 tcagccacgc cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag
     8941 ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc tgataattga
     9001 tcgtcaggga atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga
     9061 ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg
     9121 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     9181 gcaggggact aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg
     9241 ctccgccctc ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc
     9301 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     9361 accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     9421 atacctgtcc gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg
     9481 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt
     9541 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cgggaaagca
     9601 cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
     9661 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
     9721 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa
     9781 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc
     9841 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca
     9901 gttcagagga ggacgatggt gccaccgttc cgccctgaac atcccggata taatttgcct
     9961 gacatgcggc cagaagaaag ccagcaacaa tcaatataaa aagaaaaata gtctttacct
    10021 ttttcatata ctttccctgt atttaaagac tccggcattc atccctgaac accggaagtc
    10081 agtatttcaa atcatccttg ataatttttt gttaccagtt accgtcccga taatcaaaag
    10141 ctatataaca attatccaca tcaggtttta tttgattttt aaaaaatggc tgtagtaaag
    10201 gaacccattc tttcttcaaa tcaaactcac cattattaat attatccttt aaaggcattc
    10261 ccaagctttc aatgacagaa ccatcgtcac caaagtcttt agaatactct tcccctttaa
    10321 actcttcagt tttcttatca aaccatgtca aacgtaattt aagccccata tttacctctc
    10381 aaagatattt tttaatgttc cgttttggat ctggcccttt aacctgctta cccgttttgg
    10441 gatcaaatgc gcctaggtgt tcgccatcac tggcacgata cccttcaagc tcaccgtgct
    10501 gggaatccca ttcataaatt ttacgttttt tatcgccata ccatcgggcc cgcttaccac
    10561 caccaccttg tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt ccctttatgt
    10621 cttctgtttt aggtactgga tgataatcat ggccgtagtc tttagtcccc ttgcgaggct
    10681 tgttcttttc ctcatttaac ttattttctg catccttcgc ttttttctcc ttttgtttcc
    10741 ggctctccat cgccccggta agtgcagcct tatctctgga taaggattca ttcattgcgt
    10801 ttaaagatgc ctgagccgct tttaatttct cattctgatt ctgaatatcc ttatcaagat
    10861 agcgaacctg atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat
    10921 catctttatt tgcctctgcc tcttttacct ttttatcggc ttcctgaaca gcagcccttg
    10981 caccggggag cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca atatttttat
    11041 cttcggcatc cagctcagct ttcgctttat catagtctct tttcagcccc tcttccggat
    11101 gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt tttacctgtg
    11161 ccggggtaag aacatctgtc accgacacat aaaccggctt ctgtccggtc tctttcggga
    11221 aacgaataac ggcctcacgg gagttaccac cggctgtaaa accagccgga cgtgaatcac
    11281 ctttttcagc aacaatgcct tttggcgggg ctttcgctgt cggaacacct ttcggtacgc
    11341 tcacctgcag agacgggaga cccggaatcg acacactgaa tacccccgga cgttttgtcg
    11401 gtttcgcatc caccacaggg acactcattg gccgacctgc gacaaccgca atatgctgcc
    11461 gctcatcctt caccacatcc acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg
    11521 gtaaagtact tgccggagtc tccgtcaccg catcggccgg taatgacgtc ataatttttg
    11581 acatcatttt cggatcatct tttgcaatct cagaaggcag cacaccgtag atcgcaatcc
    11641 cccacagacc aaacttaaac ggccccttca gggcagccag cacatcagca acggcggccg
    11701 acaacgcatc gccggaaact gataaagcca gtccttcagc ccccggagta gccagggcag
    11761 gcagaccgaa ggccatcgca gtggccgaag actgtccgcc accagagcta ccgctatgat
    11821 tgccgctgtt accaccatta ccaccgggtt tactcccgcc gttaccaaac tcccccggat
    11881 tatagttatg aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg
    11941 gatccgtacc accacctgat gaagggccac
//
