LOCUS       NZ_CP029366             2101 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain WCHEC035148 plasmid p2_035148, complete
            sequence.
ACCESSION   NZ_CP029366
VERSION     NZ_CP029366.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09202772
            Assembly: GCF_003268695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,K., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC035148
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Ma,K., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268695.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:45:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,870
            CDSs (total)                      :: 4,745
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 71 of 236
            Pseudo Genes (incomplete)         :: 160 of 236
            Pseudo Genes (internal stop)      :: 44 of 236
            Pseudo Genes (multiple problems)  :: 35 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029366.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC035148"
                     /host="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="p2_035148"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            complement(join(1153..2101,1..17))
                     /locus_tag="DI496_RS00555"
                     /old_locus_tag="DI496_00555"
     CDS             complement(join(1153..2101,1..17))
                     /locus_tag="DI496_RS00555"
                     /old_locus_tag="DI496_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(883..1149)
                     /locus_tag="DI496_RS00565"
                     /old_locus_tag="DI496_00565"
     CDS             complement(883..1149)
                     /locus_tag="DI496_RS00565"
                     /old_locus_tag="DI496_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(1494..1725)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aatttatctt ctgacattaa gcctgctccg atctgggcag gctgtgattt gatgtggtat
       61 attcatcacg tacctgtgca ttgcgagcca gtacatgtca ccagagtttg ccgctgaggt
      121 gacacattcg aaatgttggg gagtgttgac gcactcccca acgccgatta aaaacgcctc
      181 aattctgctc cattttcttt gacctgtcaa aacccactca aagcctcgtc attactgggc
      241 taaaccacta tttcatatcc gccatttatc ccttaagtat aaatacaagg atcacgcgcg
      301 agaattgcgc aaaaaataac cacaatttta accccccaaa acaagcaccc ccgaacggct
      361 taccccaacc ttttccacac acccgatcac caaaacaggg aataaaaaca aaaccctgtc
      421 ttacaggtgg atggcctcac ttcgtgaaac catccacccc gcataagttt tttaatcggt
      481 ggtgtcgtca atcactaaat cggaacccca aaggggaccc ccacgattta gcatttgacg
      541 agattaactc tcggagatgg catcatgctt ttaacggact ttaaaaaacg gtgaacggat
      601 gcggatttta atccgtgtct gtgaggcgtt gagcaacgag caaagcgagg cggtagaaag
      661 agagccagcg gcaggctcag gacgataatc cggggaaaat gtaccactcg cagagtgtta
      721 acatttgaca tcggattgtc tatcctgcat tggtgtcgag ttggcaaggc tgaggataat
      781 gctgaggata atgctgagga taatgcagga tagacagagc actagagatt gcatgcaatc
      841 tcgtacctat cacctcagcc tgccgctggc tcttttccag aactaagaat gcgttcttaa
      901 aaatccaaac ttgataccgt ttaaatctat tttcacaaaa agagttttta ccctctcgtt
      961 tgttttctga atatataaaa ttaattcatc ctcaaaacct tcgtgcggat gaaaaccaaa
     1021 tgtgccaaaa cttaatccat caataagcaa aataaggttt ttaaacctgt caggaatcac
     1081 cgcttcactt cttaaaaaaa ataaaccaat agtcttattt ccattcaggt attcaatatt
     1141 aatattcata cgttaatccg atttatcctt ctcaggggcg cgtttgtaac gccgtttacc
     1201 tgaatcccag acaaacgccg tccgcttccc gtcatcagtg ccatccccta catcgtcggc
     1261 aatgacaaga tcctcattgg tttctcgatc caactggagg acgtttttta gcgccccacc
     1321 ggtcgagata aaacggcgct tgtgaagctg ccgcgtcagc tcaagaaacc actcaggatc
     1381 gtttgccata tcttccggtt taacggagta tttcagcgtt tcagcaaccg cgctttgcag
     1441 ttgctcggca acgttggcca caacctcacc tgtcttagtt tttactgccc gaatatcgat
     1501 attcggctca tagttcaccc gcaagcaatc gcgccagagt tctacccaac gttcgtgctt
     1561 aacgtagttc ttccctttaa accaagaagg ttgcaccatc agcagacagt gaaaatgcgg
     1621 atgtgcgctg ccatccttac ctcgcgtcac ctccgtagcc ctgatccacc cctgaacagg
     1681 tgatagctcc tttcgctttt ccattcgctt aaacgccgca ttcattgctg taaggactgt
     1741 tccaagttca cctatctcgc agttcctgac agttaacgtc agaaacaacc atcgggaaga
     1801 cgggtaatcc acaacgattt tcggtagagc ctgataaaaa cgggcttgcc acatgagggt
     1861 tcttctccac tggcagacag ggcaatgacg gacacggcaa aattgcgcac tacgaagccg
     1921 taggcgcgtt tctccggttt ccatgatggt agaccaacca aaacgtaaca aaccgctaca
     1981 ggaggccata cggagtgcat agcgctcgaa ttcagcagca gtctggtaga tacccccgac
     2041 agaatcggta agcgtgcgct gggtatccca aactgcatca cgggggctgt agtccgaaag
     2101 g
//
