LOCUS       NZ_CP029125             4096 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain AR432 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP029125
VERSION     NZ_CP029125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291525
            Assembly: GCF_003073995.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 16.36x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 02:01:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,689
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,498
            CDSs (with protein)               :: 4,498
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 42 of 76
            Pseudo Genes (internal stop)      :: 7 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            Pseudo Genes (short protein)      :: 1 of 76
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="AR432"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
     gene            25..258
                     /locus_tag="CSC19_RS00020"
                     /old_locus_tag="CSC19_0001"
     CDS             25..258
                     /locus_tag="CSC19_RS00020"
                     /old_locus_tag="CSC19_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            280..507
                     /locus_tag="CSC19_RS00025"
                     /old_locus_tag="CSC19_0002"
     CDS             280..507
                     /locus_tag="CSC19_RS00025"
                     /old_locus_tag="CSC19_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1037..1246)
                     /locus_tag="CSC19_RS23950"
     CDS             complement(1037..1246)
                     /locus_tag="CSC19_RS23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1250..1735)
                     /locus_tag="CSC19_RS23955"
     CDS             complement(1250..1735)
                     /locus_tag="CSC19_RS23955"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1770..2474)
                     /locus_tag="CSC19_RS24365"
                     /pseudo=""
     CDS             complement(<1770..2474)
                     /locus_tag="CSC19_RS24365"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2413..2736)
                     /locus_tag="CSC19_RS00035"
                     /old_locus_tag="CSC19_0004"
     CDS             complement(2413..2736)
                     /locus_tag="CSC19_RS00035"
                     /old_locus_tag="CSC19_0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3375..3533
                     /locus_tag="CSC19_RS24370"
     CDS             3375..3533
                     /locus_tag="CSC19_RS24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(3770..4039)
                     /locus_tag="CSC19_RS23965"
     CDS             complement(3770..4039)
                     /locus_tag="CSC19_RS23965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     Typing          complement(2845..2923)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          2999..3487
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(928..2423)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttacctttaa gcagggaacc tattatgaat aaaaaaacgc tgttggcggc catgatggtc
       61 gcgttggtga cctttgccgc tgctggatgt tcttcaaacc aagaaataaa aaccacagat
      121 gatcgcacta tcgttacaga cggcaaaccc aaagttgaca gtgatacggg attagtatcc
      181 tacaaagatg cccatacagg ccgaaccgaa cagatcaacc gtgacaaaat taccaacatg
      241 agtgaactag acaactgata cggataaaag gagctggctt tgaaaaagat ttttgtttca
      301 gtgttcgcag cggcagttgc gctttcagcc ctaaccggct gcacgcgcac aagctatgcc
      361 atccacacca acgacgggcg tacaattgtc agcgacggca agcctacaga atcagactca
      421 ggcctgctgg gctataaaga cgcaaatggt gtcaaacagc agatcaataa agccgacgtt
      481 aaagaagtct ctgaaattcc gcattgaggc atcgctctct ccgaaaaggg ccgctaatgc
      541 ggcccttttc tttatctgcc ccaacaacag gcaaaaaaag cccccgctgg gggccaaata
      601 cctgcagtgg ctggatagta ctgcattcct gatgaaggag acgaataagt atgggccgga
      661 tttttgcgtc tgcaggctcg gtcgccacaa tttttccgta cccgccaggc atcatgcttt
      721 taccataaca tcccttgccc gcacctctcc ggacgggttc agcgcgaatg cggcgtcagt
      781 cctgcaaaac cgacagtaaa ccaccccgca aaccgtgcat tctgcgcctg ataaatctca
      841 cccgactgca tagccatgca gccgggtgaa tggcaccggg tttcggcgca gatcctcaat
      901 gaaagccccg ttttatccgg ttatcacagc gacggaccgt catactcctg ctgacgctgc
      961 gatcgttcca gctccagcgc tttctggtgc tgcgctgcgc gctcctgctc ccggtgtgcc
     1021 tcgatgtcgt tcagccttac gacaaccggc gcagctgctc gcttaaactc agcacctgcg
     1081 tgctcaagtc cgtgacgcgc tcgctcagcg ctgcgttctc ccgttgcata agaccacaca
     1141 tcgtccgcca ttccgcgaag gcgctctccc actcgtccag ccttttcgag tagtcctgct
     1201 gtagctgctc taatgcgctc agcaactgtt tttccagttc cgtcatgtgc tatttccccg
     1261 ccagtatcat ccagctcgaa tcctctccga accgtcccgc gtccgggttc accctcacgc
     1321 agcggcgtcg ttcctctccg caggtcgtga gctgcacgcc gctgttcctc tcggacagca
     1381 tggcctgcgt gctcttctgc tcccggatgg tcgtgtaatt gtcgagtatc tgctgcccct
     1441 gccaccatag aatgcccgca cttgaggcta tcagcagcac ggagaccagg acgatggtca
     1501 gccacgtctg gctgaccatg cgcagcatcc ctttcgtacg ctggctcatg gctgaggaca
     1561 gcatccggtc gtggtcgtgg atggcggcgc tgattctctt ctcgctttcg ttcagttccg
     1621 ctctgacaga cttctcgtgc tcgctgaatg cggctttcag catctcgccg gtagtctgct
     1681 gctgcacttt cgatttctgc tctaagtcct tcgccagcgt taaaagactg ttcatagatg
     1741 gctcccttca gtcggatgtt tcgccccccg tccgggtcgg caatgctgat gctgcttttt
     1801 gtggtgcgaa ccacctcaaa acctgcgctt tccagcgcct cagtgacgtc ctgacgcgtt
     1861 ttcagctccc cggacgaggc taaggccagt aaaccccgcg taatcgcctg agcggcttcc
     1921 tgcttcgttt ccggcagcgc ggacggcgtg accagtgccc gccggttttc cggcgcgttc
     1981 gggtcgtgca gccccagact gccgttcacg atggtctgcc aggcatcaat gcgaggacgg
     2041 tcggcgcggt catagtatgg ctggagccgt ttgccggtca gcagctccgt gttcgggatc
     2101 aggaagttca gctccagccg ccccttgtcc cggtgctcaa cccacagcac gctgtactgg
     2161 tctttatcga gtccgggcat cagaacccgc tcgaagctcg ccatcagcgt ttcacgctgc
     2221 ccgggcggta agtcctgttc ggcaaaggac agcaccccgg aggtgtactt tttggcgtag
     2281 ggcgaggcgt cgataagctc ccggacttcc tccggcttcc cctgaagaac gctggcaccg
     2341 tcgcgctggc ggtctttccc cagcagataa tcaaccgggc cggcaccgcc tccgcgtccc
     2401 cggggatgaa acttaacgat catcgtcagc ccccttttcc agtacggcat gccgcagccg
     2461 ctcgagtccg gcatcgatgg ccatcagcgc ggcgacgacc tgcacgcggt cgagaccgct
     2521 actgccaccg gcgttaacct ggcgggcaat ctggttgagg ttgttcccca tgccggcaag
     2581 ctgacgaagc agcgccggcg agatcgaggg aagtttgccg gcccgggcgg gcttctcgtc
     2641 caggcacgtc tgccgcatcc aggccgcgag ctgtttaccg tcgcagcgtt ccagcagacg
     2701 gcggtgctca tcttctgtta cccacatcgt gagcattttg ctgcgtttgt ctgccaatcc
     2761 ggcctcctga taacgccggg acgggcggga aatcttttcc cggtcggcgc ggtgttgggc
     2821 aagccgcagg cgcgtcagtg gcatttagcg ggtttcgggg cgcagccctg aaccagtcac
     2881 gtagcgctag cggagtgtat actggcttac tatggtggca cgacgggaat cgggatgaaa
     2941 tagtcagtca tgaaaactgt gagatgaggc tcaggtgcgt aagaggctga tgcctgtccc
     3001 ggtggatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga
     3061 gcggtacgga ctcacggata aagacgtggc ggggaaatac gtgaatgcag gaggggaaga
     3121 aaggatgcgc cgctgccggt tttccatagg ctccgccccc ctgacaagca tcacagaatc
     3181 tgacgctcaa atcagtggtg gcgaaacccg atgcggctta aagataccag gcgtttcccc
     3241 ctgatggctc cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt gtcattccgc
     3301 tgttatggcc gcggtttctc attccacgcc tgacactcag ttccgcgaag gcagttcgct
     3361 ccaggattga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta
     3421 actatcgtct tgagtccaac ccggaaagac acgaaaaatc gctactggca gcagccatta
     3481 gtaactggag gtatacgaga gacaaattaa gaggtcttga agtatgggcc tgagagaggc
     3541 tacactgaaa gaacagtttt ggtttctgcg gtctcgtata cccagttacc tggttaagca
     3601 gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgttttca
     3661 gagcaagaga ttacgactgt aacgacagga tcacaagaag atcatttact cagagagact
     3721 aactaccact atgtttgcct atcaacatac ttaggaaaca ttacttgagt taacgactcc
     3781 accttacttt tagtatctcg caatgaatct tccgaccgcc gttgtgttga agttctattg
     3841 cgccaccggg gtataaatca ctttcgttta actcaattaa aagctcgttc ttgtgagcat
     3901 cctctaaata aaggtaataa cacccaccat aaaggttctg cgtatctggc acggcaacaa
     3961 aagatttctt tccgaacagt aaaacagcct ctttttctcc atgctctgtc agcaataatc
     4021 gaaattcttg tccattcaat cctcttttcg catgaaaaaa cgacctatac ttagatggtt
     4081 agtgaccata tccact
//
