LOCUS       NZ_CP029119            35284 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR435 plasmid unnamed6.
ACCESSION   NZ_CP029119
VERSION     NZ_CP029119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291528
            Assembly: GCF_003073835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35284)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35284)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 14.36x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003073835.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:07:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 4,858
            CDSs (with protein)               :: 4,858
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 107 of 294
            Pseudo Genes (incomplete)         :: 192 of 294
            Pseudo Genes (internal stop)      :: 63 of 294
            Pseudo Genes (multiple problems)  :: 61 of 294
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..35284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR435"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
     gene            complement(252..752)
                     /locus_tag="CSC22_RS27705"
                     /old_locus_tag="CSC22_5395"
     CDS             complement(252..752)
                     /locus_tag="CSC22_RS27705"
                     /old_locus_tag="CSC22_5395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="WP_000117626.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRALH
                     QDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETDFD
                     TFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFIDGHV
                     FRR"
                     /name="antirestriction protein ArdA"
     gene            complement(1214..1810)
                     /locus_tag="CSC22_RS27715"
                     /old_locus_tag="CSC22_5396"
     CDS             complement(1214..1810)
                     /locus_tag="CSC22_RS27715"
                     /old_locus_tag="CSC22_5396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000977995.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANVV
                     RYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGERMA
                     ARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVMLIKR
                     LASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
                     /name="hypothetical protein"
     gene            complement(1807..2526)
                     /locus_tag="CSC22_RS27720"
                     /old_locus_tag="CSC22_5397"
     CDS             complement(1807..2526)
                     /locus_tag="CSC22_RS27720"
                     /old_locus_tag="CSC22_5397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="WP_047149313.1"
                     /translation="MSARSRALIPLNAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAFF
                     RCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAGHR
                     LFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPATVG
                     SWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRGAPVQ
                     VRALEQWMIPNKLENVI"
                     /name="plasmid SOS inhibition protein A"
     gene            complement(2523..2957)
                     /gene="psiB"
                     /locus_tag="CSC22_RS27725"
                     /old_locus_tag="CSC22_5398"
     CDS             complement(2523..2957)
                     /gene="psiB"
                     /locus_tag="CSC22_RS27725"
                     /old_locus_tag="CSC22_5398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="WP_001402001.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPAN
                     WMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVVQV
                     QHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
                     /name="psiB"
     gene            complement(3010..>4299)
                     /locus_tag="CSC22_RS27730"
                     /old_locus_tag="CSC22_5399"
                     /pseudo=""
     CDS             complement(3010..>4299)
                     /locus_tag="CSC22_RS27730"
                     /old_locus_tag="CSC22_5399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(<4300..4374)
                     /locus_tag="CSC22_RS27735"
                     /pseudo=""
     CDS             complement(<4300..4374)
                     /locus_tag="CSC22_RS27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /name="single-stranded DNA-binding protein"
     gene            complement(4758..5072)
                     /locus_tag="CSC22_RS27745"
     CDS             complement(4758..5072)
                     /locus_tag="CSC22_RS27745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001434357.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRPL
                     RLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPVAP
                     "
                     /name="hypothetical protein"
     gene            complement(5143..5334)
                     /locus_tag="CSC22_RS27755"
                     /old_locus_tag="CSC22_5401"
     CDS             complement(5143..5334)
                     /locus_tag="CSC22_RS27755"
                     /old_locus_tag="CSC22_5401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001027495.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVAN
                     QQAVFLGGHYILQGSRNR"
                     /name="hypothetical protein"
     gene            complement(5331..5753)
                     /locus_tag="CSC22_RS27760"
                     /old_locus_tag="CSC22_5402"
     CDS             complement(5331..5753)
                     /locus_tag="CSC22_RS27760"
                     /old_locus_tag="CSC22_5402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_000271762.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTDD
                     ELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEMKR
                     LYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
                     /name="DUF1380 family protein"
     gene            complement(5800..6225)
                     /locus_tag="CSC22_RS27765"
                     /old_locus_tag="CSC22_5403"
     CDS             complement(5800..6225)
                     /locus_tag="CSC22_RS27765"
                     /old_locus_tag="CSC22_5403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_001198928.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMPE
                     YQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLWLY
                     HDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
                     /name="antirestriction protein"
     gene            complement(6643..7413)
                     /locus_tag="CSC22_RS28475"
                     /old_locus_tag="CSC22_5404"
     CDS             complement(6643..7413)
                     /locus_tag="CSC22_RS28475"
                     /old_locus_tag="CSC22_5404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011264064.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVRL
                     DAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSFPY
                     AGEWLAEDEIRAVLAAVRDAVRCVSYQVADDARRIRAALTTTGQTLLTRQTRRFRLVVK
                     ESDHPCWLDEDDENLPVVLDAIVNRGARFSAVEMYLVSECVEHILSSGLACDVLRIPDE
                     PPRRWFDRDVLREVVREARAEIRSMADALAKIRK"
                     /name="hypothetical protein"
     gene            complement(7458..7976)
                     /locus_tag="CSC22_RS27780"
                     /old_locus_tag="CSC22_5405"
     CDS             complement(7458..7976)
                     /locus_tag="CSC22_RS27780"
                     /old_locus_tag="CSC22_5405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_202864534.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQYP
                     GNTPLMLVVWSPEEIQALADGMDIALTDHEIRTVLARLEDIPEDQRIESGISSAAVMEI
                     IRNESENRLVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARAL
                     LKNNTREND"
                     /name="DUF1380 family protein"
     gene            complement(7906..8127)
                     /locus_tag="CSC22_RS27785"
                     /old_locus_tag="CSC22_5406"
     CDS             complement(7906..8127)
                     /locus_tag="CSC22_RS27785"
                     /old_locus_tag="CSC22_5406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001104873.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERL
                     TRQTLYRANRLFMEAYTEILELDSCFKD"
                     /name="hypothetical protein"
     gene            complement(8128..8811)
                     /locus_tag="CSC22_RS27790"
                     /old_locus_tag="CSC22_5407"
     CDS             complement(8128..8811)
                     /locus_tag="CSC22_RS27790"
                     /old_locus_tag="CSC22_5407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="WP_000086127.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAGD
                     KTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYTSK
                     AAYVGYRHECAYILAKGRPALPQKPLPDVQGWKYSGNRHHPTEKPVTSLQPLIESFTHP
                     NAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAANDNWF
                     EPEAA"
                     /name="DNA methylase"
     gene            complement(8887..9197)
                     /locus_tag="CSC22_RS27795"
                     /pseudo=""
     CDS             complement(8887..9197)
                     /locus_tag="CSC22_RS27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(9201..9623)
                     /locus_tag="CSC22_RS27800"
                     /old_locus_tag="CSC22_5409"
     CDS             complement(9201..9623)
                     /locus_tag="CSC22_RS27800"
                     /old_locus_tag="CSC22_5409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_000774832.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLCG
                     AALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNNAK
                     AILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(9626..10606)
                     /locus_tag="CSC22_RS27805"
                     /old_locus_tag="CSC22_5410"
     CDS             complement(9626..10606)
                     /locus_tag="CSC22_RS27805"
                     /old_locus_tag="CSC22_5410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="WP_012783921.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTDA
                     TVPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVDIV
                     VTLPLGEYLDENDQPNLANIERKKNNVRRTVTVQGRENFTIRKVSVLPESVPAGFDVLK
                     DLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVNVSTR
                     TSSYFADRLIQGRDDNEFLTRYIPNAEQRERILTVLRDREKKLHYRVADSVGRFAGFTH
                     VMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
                     /name="plasmid segregation protein ParM"
     gene            10944..11279
                     /locus_tag="CSC22_RS27810"
     CDS             10944..11279
                     /locus_tag="CSC22_RS27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001340323.1"
                     /translation="MRTITTREQLLVNGKVRERIATHIVTGAHGYETLCTSGYNLQYNK
                     ERVLIENCEKVADGELPVTCHTCFSIWQDVHRFKPGDFDTESGKGNFTDTELTKITIGQ
                     EKTPNAC"
                     /name="hypothetical protein"
     gene            complement(<11284..11370)
                     /locus_tag="CSC22_RS29235"
                     /old_locus_tag="CSC22_5412"
                     /pseudo=""
     CDS             complement(<11284..11370)
                     /locus_tag="CSC22_RS29235"
                     /old_locus_tag="CSC22_5412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(11374..12276)
                     /locus_tag="CSC22_RS27815"
                     /old_locus_tag="CSC22_5413"
     CDS             complement(11374..12276)
                     /locus_tag="CSC22_RS27815"
                     /old_locus_tag="CSC22_5413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="WP_001739825.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLLQ
                     ITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTG
                     QRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDLLTV
                     LPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDFDTPW
                     CQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSPTDDDE
                     LPEVTGPAWIVDKVAHYGG"
                     /name="DUF1281 domain-containing protein"
     gene            12694..12942
                     /locus_tag="CSC22_RS27825"
                     /old_locus_tag="CSC22_5414"
     CDS             12694..12942
                     /locus_tag="CSC22_RS27825"
                     /old_locus_tag="CSC22_5414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="WP_000618110.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRIDQ
                     GSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
                     /name="DinI-like family protein"
     gene            12939..13376
                     /gene="umuD"
                     /locus_tag="CSC22_RS27830"
                     /old_locus_tag="CSC22_5415"
     CDS             12939..13376
                     /gene="umuD"
                     /locus_tag="CSC22_RS27830"
                     /old_locus_tag="CSC22_5415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_000109071.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDLN
                     SYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLL
                     LRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
                     /name="umuD"
     gene            13376..14647
                     /locus_tag="CSC22_RS27835"
                     /old_locus_tag="CSC22_5416"
     CDS             13376..14647
                     /locus_tag="CSC22_RS27835"
                     /old_locus_tag="CSC22_5416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_001703910.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIKMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPDFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGTVWFAGRGTAPE
                     WQMKREMLSPAYTTRWTDLPVAQL"
                     /name="Y-family DNA polymerase"
     gene            14835..15461
                     /locus_tag="CSC22_RS27840"
                     /old_locus_tag="CSC22_5417"
     CDS             14835..15461
                     /locus_tag="CSC22_RS27840"
                     /old_locus_tag="CSC22_5417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="WP_000587689.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVLT
                     WYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVFIS
                     PLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKDYPE
                     MHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
                     /name="ParA family plasmid-partitioning AAA ATPase"
     gene            15458..15760
                     /locus_tag="CSC22_RS28725"
                     /old_locus_tag="CSC22_5418"
     CDS             15458..15760
                     /locus_tag="CSC22_RS28725"
                     /old_locus_tag="CSC22_5418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001245884.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSIS
                     FFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
                     /name="hypothetical protein"
     gene            complement(16251..17597)
                     /gene="yagA"
                     /locus_tag="CSC22_RS27855"
                     /old_locus_tag="CSC22_5419"
     CDS             complement(16251..17597)
                     /gene="yagA"
                     /locus_tag="CSC22_RS27855"
                     /old_locus_tag="CSC22_5419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="WP_039052405.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMAQHNLAN
                     DLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLSGN
                     EEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAYHNGERSSVRKKRPRLGRLL
                     LSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPGMSER
                     SQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVFRPVRGISIN
                     GEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTLTALLNV
                     ASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTAMAPDGKTARVVIETMGYE
                     DSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
                     /name="yagA"
     gene            17951..18553
                     /locus_tag="CSC22_RS27865"
                     /old_locus_tag="CSC22_5420"
     CDS             17951..18553
                     /locus_tag="CSC22_RS27865"
                     /old_locus_tag="CSC22_5420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_001703912.1"
                     /translation="MEKNQEERKIPVIVVKKRRTFSLPSLSEKTDIIESAFTEQAAESA
                     PVGINSSAVETHIPEAPARKKKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEGGG
                     FIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDGH
                     PKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
                     /name="ProQ/FINO family protein"
     gene            18609..19118
                     /locus_tag="CSC22_RS27870"
                     /old_locus_tag="CSC22_5421"
     CDS             18609..19118
                     /locus_tag="CSC22_RS27870"
                     /old_locus_tag="CSC22_5421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012783913.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIYL
                     SGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFSILDRQSLLNLMEATKDSPDVKQELLN
                     RLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTVPNE
                     DKKGIR"
                     /name="hypothetical protein"
     gene            complement(19351..19491)
                     /locus_tag="CSC22_RS28480"
                     /old_locus_tag="CSC22_5422"
     CDS             complement(19351..19491)
                     /locus_tag="CSC22_RS28480"
                     /old_locus_tag="CSC22_5422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032072206.1"
                     /translation="MAENGYGLAGLGMGKVKSISPYRLTPGFGGFTPVSYATTECRLPC
                     R"
                     /name="hypothetical protein"
     gene            complement(20187..21218)
                     /gene="repA"
                     /locus_tag="CSC22_RS27890"
                     /old_locus_tag="CSC22_5423"
     CDS             complement(20187..21218)
                     /gene="repA"
                     /locus_tag="CSC22_RS27890"
                     /old_locus_tag="CSC22_5423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_023518831.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERKR
                     TKRRRGEHSTKPKCENPSWYRPELYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSLHP
                     FYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKDSKG
                     KVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQMLGVD
                     MVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHRRAKAA
                     ASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLELTLAAP
                     PPH"
                     /name="repA"
     gene            22175..22453
                     /locus_tag="CSC22_RS29420"
     CDS             22175..22453
                     /locus_tag="CSC22_RS29420"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="WP_219334566.1"
                     /translation="MWRRFLPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWA
                     ITNERRNSVIAFFVVIGDLCYVTSNDTETPGHHSVGRHVSRAINKPA"
                     /name="ash family protein"
     gene            22371..22658
                     /locus_tag="CSC22_RS27910"
                     /old_locus_tag="CSC22_5424"
     CDS             22371..22658
                     /locus_tag="CSC22_RS27910"
                     /old_locus_tag="CSC22_5424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="WP_000074855.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQN
                     CRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
                     /name="conjugal transfer protein TraA"
     gene            23100..>23405
                     /locus_tag="CSC22_RS27920"
                     /pseudo=""
     CDS             23100..>23405
                     /locus_tag="CSC22_RS27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            complement(23419..24116)
                     /locus_tag="CSC22_RS27925"
                     /old_locus_tag="CSC22_5425"
     CDS             complement(join(23419..23867,23867..24116))
                     /locus_tag="CSC22_RS27925"
                     /old_locus_tag="CSC22_5425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<24172..25329)
                     /locus_tag="CSC22_RS27935"
                     /old_locus_tag="CSC22_5427"
                     /pseudo=""
     CDS             complement(<24172..25329)
                     /locus_tag="CSC22_RS27935"
                     /old_locus_tag="CSC22_5427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            complement(25482..25856)
                     /locus_tag="CSC22_RS27940"
                     /old_locus_tag="CSC22_5428"
     CDS             complement(25482..25856)
                     /locus_tag="CSC22_RS27940"
                     /old_locus_tag="CSC22_5428"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223349261.1"
                     /translation="MSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLFIDR
                     KDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLSEDII
                     SDVANTYVDNLDSTSVLQKA"
                     /name="hypothetical protein"
     gene            26177..26329
                     /locus_tag="CSC22_RS27945"
     CDS             26177..26329
                     /locus_tag="CSC22_RS27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001387489.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLA
                     YEVKR"
                     /name="Hok/Gef family protein"
     gene            complement(26401..26652)
                     /locus_tag="CSC22_RS27950"
                     /old_locus_tag="CSC22_5429"
     CDS             complement(26401..26652)
                     /locus_tag="CSC22_RS27950"
                     /old_locus_tag="CSC22_5429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001291964.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAFT
                     LMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
                     /name="hypothetical protein"
     gene            27153..27248
                     /gene="dqlB"
                     /locus_tag="CSC22_RS29425"
     CDS             27153..27248
                     /gene="dqlB"
                     /locus_tag="CSC22_RS29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609148.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELID"
                     /name="dqlB"
     gene            complement(27313..27489)
                     /locus_tag="CSC22_RS28730"
                     /old_locus_tag="CSC22_5431"
     CDS             complement(27313..27489)
                     /locus_tag="CSC22_RS28730"
                     /old_locus_tag="CSC22_5431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001054897.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESKE
                     QALEVKEGYKFLT"
                     /name="hypothetical protein"
     gene            27881..28090
                     /locus_tag="CSC22_RS27960"
                     /old_locus_tag="CSC22_5432"
     CDS             27881..28090
                     /locus_tag="CSC22_RS27960"
                     /old_locus_tag="CSC22_5432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_000062603.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITREL
                     HRLSQSDDMEIKIAAIKALGRIYR"
                     /name="HEAT repeat domain-containing protein"
     gene            complement(28162..28824)
                     /gene="excA"
                     /locus_tag="CSC22_RS27965"
                     /old_locus_tag="CSC22_5433"
     CDS             complement(28162..28824)
                     /gene="excA"
                     /locus_tag="CSC22_RS27965"
                     /old_locus_tag="CSC22_5433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="WP_000644796.1"
                     /translation="MKAKKETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEMLC
                     SDRYFGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEKGY
                     EGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTKYIN
                     LPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGIPVAEVF"
                     /name="excA"
     gene            complement(28895..>30649)
                     /locus_tag="CSC22_RS27970"
                     /old_locus_tag="CSC22_5434"
                     /pseudo=""
     CDS             complement(28895..>30649)
                     /locus_tag="CSC22_RS27970"
                     /old_locus_tag="CSC22_5434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /name="DotA/TraY family protein"
     gene            complement(<30648..30764)
                     /gene="traH"
                     /locus_tag="CSC22_RS28485"
                     /pseudo=""
     CDS             complement(<30648..30764)
                     /gene="traH"
                     /locus_tag="CSC22_RS28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /name="traH"
     gene            complement(31159..31743)
                     /locus_tag="CSC22_RS27975"
                     /old_locus_tag="CSC22_5435"
     CDS             complement(31159..31743)
                     /locus_tag="CSC22_RS27975"
                     /old_locus_tag="CSC22_5435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_108703259.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEINA
                     DNTVIFLFRHGERCDRSDMPCYSDKSGVTITGTEKAQQEGIKFATIFSEYDIYSSNAVR
                     TIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLARDRL
                     GKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
                     /name="histidine phosphatase family protein"
     gene            complement(31803..33005)
                     /locus_tag="CSC22_RS27980"
                     /old_locus_tag="CSC22_5436"
     CDS             complement(31803..33005)
                     /locus_tag="CSC22_RS27980"
                     /old_locus_tag="CSC22_5436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="WP_000976351.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASSH
                     YGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVDNQ
                     HGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGKVND
                     ADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGVTPKY
                     QRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQNIIPRS
                     IDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFASVGAEF
                     NAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGAMAQLQFT
                     F"
                     /name="conjugal transfer protein TraF"
     gene            complement(33091..33915)
                     /gene="traE"
                     /locus_tag="CSC22_RS27985"
                     /old_locus_tag="CSC22_5437"
     CDS             complement(33091..33915)
                     /gene="traE"
                     /locus_tag="CSC22_RS27985"
                     /old_locus_tag="CSC22_5437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="WP_023518851.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGVR
                     EVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPLLA
                     TLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEWPDT
                     ADNFRMFINDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLSPTLL
                     NDEKRKWLTEYTTRQNTTMSLRRITLRPGLAGNLITGQILPVSLFNTSLLPW"
                     /name="traE"
     gene            complement(34066..35220)
                     /locus_tag="CSC22_RS27990"
                     /old_locus_tag="CSC22_5438"
     CDS             complement(34066..35220)
                     /locus_tag="CSC22_RS27990"
                     /old_locus_tag="CSC22_5438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_001139955.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPIA
                     LRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTCRT
                     NPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEILALR
                     WEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKNAWRI
                     ATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRAWQLVS
                     KLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKEEAVHRA
                     SEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
                     /name="site-specific integrase"
     Typing          complement(20188..21218)
                     /name="IncI-gamma/K1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgcgccag cagctgcctg tcggagtcgg gcctgacgac cggatgccgc caggcagccg
       61 ggagccgaag gtgtccccgc ctccggaaat cagaacaaca gaaacgacat aacagaaaaa
      121 tgcctcacac catgacgggc aggaagggca gcgccgcagg cgcggccctc ctgcatttca
      181 ggcaggcgta agcctgccag ggttttccgg cggcctggca ggccataccc ccgccggagc
      241 gggggagtaa atcaccgacg gaacacatga ccgtcaataa aggtgaagga gtccaggaat
      301 aaatcccgcg catacgcctc atagtcaaaa tagagcgcca ccgtctccgg cacgtcagcc
      361 agcaggccgg tatcactggc gaactcaacc gcaaaagcct cctcactgtc agcctcgccc
      421 caccaggcat cgcggaaggt gtcaaaatcc gtctcaccgg tatcatccac ccagagacga
      481 taagcctctt cgcagccttc atcccgtgcc cggcggaagc cttcaaccca ggcccagttg
      541 atatgacatt cagaggccat attccccggg aatccctcat aatcctgaaa catcagttca
      601 ggatcggctt catcctggtg aagagcacgg caggcggcga aaaagtcgcg ctcatcatca
      661 aacgtggtca ggtcaaacca gcgtccggcg atgcttccac agttgtattt gtgccaggtt
      721 ccaacgtata cagcaggtgc aacaacagac attcccgaat cctccatagt cacatgttct
      781 gcgacaccgg gcggtgccgt gcctccataa aagccgggcg ataaggcggg gatgcagggg
      841 cgcaagggaa gccgcagcct gacgcacacg gctgacggga gttgcagggt gaggccgcga
      901 aacctgagcg gacgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc
      961 gcagcggcga agcgtaccct tgcatccctg ccagcccggc taagatggag gcacggcaca
     1021 gcccgcacac gacgctgagg ccacagagct gaaaacccac aaggccatgc cccgcatggc
     1081 cgggtaaaag cggagccgcc ggcgacttcc ttattccggc gagtgcagag gacacgccgc
     1141 atgacctcca gaccacccct gatatgaaac gatcatgatc ggcgtgtccc tgcgtaaaat
     1201 atacatgcct ggttcaccgg agaatatctg tgatatccac aagttttctg tcgcgcagcc
     1261 agtcctgcgc ttcacgcacc acacaactgt ccggtttcac cgctttcagc gatgatgcca
     1321 gtcgcttgat caacatcacc agttcccgtg tctgtatgtc cggcattgtc tccggcggag
     1381 cagggggact ctcatttccg acgctcatcc catgcgcttc cagtgtggca cggacaaacc
     1441 aggcggcatc gaccagttgc tgtccaatcc aggccagatc ggcatttctg tcacgggctg
     1501 ccattcgctc gcccgacagc gccttgctga tatggctgcg caccagatta tccaggagtg
     1561 cctgcgcttc ttccggtgaa aagccacctg gtatctctgt gcgcaacagc cctttcctgt
     1621 acagaagctg cagcagatca acctgcgacc gcacagcaac tatcagtaca taacggacaa
     1681 cattcgcact ggcagtggtt tctgtatcat tcagcagttc ttccggatcg ttccccccgg
     1741 tgttgcagag caacggcaac agaggtcgca tccacgcggg aacctggtat ttttcgtctg
     1801 atttcatcat attacgttct ccagcttgtt cgggatcatc cactgctcaa gtgcacgtac
     1861 ctgtaccggt gcaccacgac cggcctcacc ggcttcaaaa atcagccgcc agagtggttc
     1921 ctcctgccag aaaaaccggt caaatgatga cagtgatgga aaacgcccgc accaccccca
     1981 gaaaatcgtt tccagatcat gctcctcaac aacaccagac cagcgggaca accagcttcc
     2041 gaccgtggcg ggagtatgaa acgcaaggtc aatctgcgcc tgagcaaaac atgtctggta
     2101 ttccagttcc cgtcgttctg cctcacgttc ccgctggcgg ctgtatttct gctctgtcag
     2161 cgtggttttc tgacgacgac gctcaccttc acgaaaaggc acggacggga acagacggtg
     2221 ccccgcatcg gatggcaggg gaagaacaca gacttcccca aaactttcaa tcagtttatc
     2281 cacggcagcc agccacagga gacggttgcc atgaaattcc tccttcgtca gcgcaggggc
     2341 aaaaaagcgg agatcggtca gcgaaatccg gcgactgcca ttcaggcagc gaaagaaagc
     2401 ggtggcataa ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt ctgtgacagc
     2461 caccgcctgc atggcggcct gttgttctgc gttgaggggg atcagtgccc gtgaacgtgc
     2521 gctcatgcct gacctccttc taccatcagg atatggatga tgtcgttaac actgtatccc
     2581 tgcgcatcca gtgtcgcggc aagcgccagc gtgtgactga tcgcgacagg cgtgaagata
     2641 tgttgcacct gaacgacggc gaaaggctgt ccaccaccat tcaccagaac cagcatccag
     2701 agctgagaga cgtctcccgg cgaacataat gccaggtgga aacgttcgtg ggctggcgtg
     2761 aaacgaacct ggacggggaa aaagcccccg aactcgctgc catactctcc gttcatcatc
     2821 cagttagccg gtgcgtccac ttcacgcatc acctcgtgag tgagattacg gcgcatatcg
     2881 cttcccgcag cacggatttc ttcatattct tgggcattca tggactgcag ggcattcagt
     2941 gtcagttcag ttttcatcac ggtatctccc tctgccgccc cgtctccggc ggggcggtgt
     3001 ggttgttcat caggcggcat cagccaggtt atcggcgcgt tcagtggcat ccgtctgtgg
     3061 acgtggtgaa cacatccagc caggcaccca gcggttgtct ttcatatgga actctgcatg
     3121 ttcagcggca tcgcctttct tcatcttctc tgcgtcccgt gcggcaccgg acagtccggc
     3181 atcattcagg gctgcgataa tctgcggctt tttcaggtgt ccgaagaagt ttgctttcgt
     3241 cggctgccac cagtcgcgca tgtgaaaacc gatggcgcta tccagtgagt caagcggact
     3301 gcgtgacgtg cgaccacact cgcgggtctg gacaccgtta aggctgcatg cggtgcagaa
     3361 actgagcagg gataacagca cctcctgact gagtgacagg aacgtcgtca tgtcccgtga
     3421 ccatccctcc ggtaacaggg ccgcaagacg ggctttttct gccatcagcg ccaggaatgc
     3481 ggcaccttcc ttccctgacg gcgcatcgct ggtcagcgaa taatgtttac attccaggct
     3541 gatttgtgct ggtttactgt acgctccgct gtcaaacaca ttcaggcaga gcgtccatgc
     3601 caacagtgca agagatttgt ccggttgctg catcagtgct gcctggactg ccagcgtgcg
     3661 ttcggaagac attttcgtca gcaacggcag actgattgcc tccactgacg ctttctcctg
     3721 cacctgttcc gtgcggtcag cgtcatcagt cgcgtcatct tcactgcgca actgcacacc
     3781 acgctggaca cacacgttgc cataacgcca ggacaccacc actccacctc cggcacgcat
     3841 ctccggcgtc cacgctctga ccttcgccat gcagcgcatc agcttcattt ctgcttccag
     3901 caggtcagat tcctcacact ggttttccag cgcgtcgtaa cgtgccatca gttcgttcag
     3961 acgttcttct tccgcctcgg tcagcaccgc ttccggctcc ggcagacagc ggtatgttcc
     4021 ggcatcctca cggcactcac cgacaggctc catgcgtccg gcgcaccatt cccagccttc
     4081 ggcttcccga aggaactctg cgacagcctg gagtttttcc agcagggcgg catcgagcgc
     4141 cacgcagtcc acatagccac cctcgtcgtc gctgaacaaa tcggtgcgca gttcgtctgg
     4201 cgagaaggca tcagccccca cgaagcggaa tttactgttc cccgccaccg ccacttcact
     4261 ttccgtaatc agattgcgga taacccgcac atccggtttg gggatgtaac ggacttccgg
     4321 atcattaccc agacgcccga cgaggatgac cttgttgata ccacgtgcac tcataagaac
     4381 ctcctgactg aaagatgtga gataaaccgg ataccggcga cgggacgttc atgtgcccct
     4441 tcagaggagc gaaagctcct cctgccgtcc acttcacagg ggtttctccc cgcagtggac
     4501 ctttccgatg ggggcccgtt taccgcctca cgggacagct gtccgggggc ggacgcaaaa
     4561 tttttagccg ggttcatggc taaaaatttt gtggccggtg catctcaccc tggacagatg
     4621 gaacgggagg gggttacgtc tgccccccgg aaagtccaca gcgggaaaca ggccgcaggg
     4681 gtggcggcag gagggaagac tgctgcgcca tcaggcgcag tttcctcaca caccgtcagg
     4741 tgtgcaggga gcgcaggtca cggcgcgaca ggggcggcgc gaccggaacg gtcgctgcaa
     4801 gccgggtttt cacgcatcgg accgtagcgg cagcccgaaa ccgcgccagc gggttcggcg
     4861 gagactggcg ggtgcgccag cagctgcctg tcggagtcgg gcctgacgac cggatgccgc
     4921 caggcagccg ggagccgaag tgggcgcaat aaaagacagc cagaaacggt gacagtgtca
     4981 cggaatatca cacctgttcc ctgcatacag ccatacacta ccttctgacc ggaaagccgg
     5041 gccggcagcg ccgcaggcgc tgcaccccgc atttccggca ggcgtcagcc tgccaggggt
     5101 tttcgcgcgg gctgacaggc ggcctccacc gcctgccctg tatcacctgt ttctgctgcc
     5161 ctgtaagatg tagtgaccgc ccaggaaaac cgcctgctga ttcgccacaa aatcgcagat
     5221 ttcatcaaca atctgcgcag tggtgagcgg tttctgcccc atttcacgac gccgcgcatt
     5281 aatcacggct ttgtaattgc gcagaatttc gcgggtttct gtgctgatat tcatgacatg
     5341 tgctccccgt ccagcgcctg taacacaacg tccattgctt cgcgttcttc cctgacgaat
     5401 gcgtcaccgt cgcccccgtt ctcgctcccg acggcataca ggcgcttcat ttcactgcct
     5461 gccagcgcca tcactttttc cagcatcacc gccggaactg tcacctgacg gctggcgcgc
     5521 ttttcttcca tcagctcacg aacaacgtcg tgaacaatgc tgacatccgc accatgcgca
     5581 aaggcatcgt ccagtcgctg catgacggtt tccagctcat cgtctgtcag actccagtcc
     5641 tgggctatgt gacgcacatc cccacgggtc agcaccacgg caaaaacgca ttcactgtcc
     5701 ggcacgtctg tgttcagcac ttcgcggata atttctttaa cagtacagta catgttctcg
     5761 tctcctttac aggggcggca ttgccgcccc gtccgttaat cagtccagtg ccgcgtaaat
     5821 cgcgttgcat tcggggtgaa attcgatgtg acgccacaac tgcgcatcgc gcatccggta
     5881 aagctgggtc agtcctgcat ccccgctgtc gtggtacagc cacaactggc ggttaatcac
     5941 aagggaagtc agaatgatgc ctgcggcatc agcactcacg gtacggtcaa accagtttgc
     6001 accgttcacc atgtggaaac ggtcgccatc cggcatcatg taaccgccac cgtccggcag
     6061 gcggatgaag tgccagaagc ctccctgata ttccggcatc attttttgcg ccagcgcgta
     6121 cacatacgcc tcggcataca gaaaatcgtt gccaaacaga tacggtagaa aggagagacg
     6181 gtcgcactct tcaacgttca gagtgacagg tttcgcatat tgcatgatgt tttcctccgt
     6241 tgttcacata ttccgcgaca ccgggcggtg ccgtgcctcc ataaaagccg ggcgataagg
     6301 cggggatgca cagggcgcaa gggaagccgc agcctgacgc acacggctgg cgggagttgg
     6361 agggtgagac cgcgaaatct gagcgggtga acgaccggaa aatcacggca gacgggaagg
     6421 agggaccggg tgaccgcagc ggcgaagcgt acccttgcat ccctgccagc ccggctaaga
     6481 tggaggcacg gcacagcccg cacacgacgc tgaggccaca taactgaaaa accacaaggc
     6541 catgccctgc atggccgggt aaagcggagc cggcggcgac ttcctttgtg ctctgtccgg
     6601 ggaccggcag gaaaaaggcg gggatttacc ccgccaacgt catcatttcc ggatttttgc
     6661 cagggcatcc gccatgctgc ggatttcggc ccgggcttcc cggaccacct cccgcagaac
     6721 atcacggtca aaccagcggc ggggcggttc atccggtata cgcagcacat cgcaggccag
     6781 cccactggac aggatatgct ccacacactc actgaccagg tacatttcca ctgccgaaaa
     6841 acgtgctccc cggttcacga tggcatcgag caccacgggc agattttcgt cgtcttcatc
     6901 gagccagcag gggtgatcgc tttccttcac aaccagacga aagcggcgcg tctggcgggt
     6961 cagcaacgtc tgaccggtgg tggtcagcgc cgcccggata cgccgcgcat cgtctgccac
     7021 ctggtaactg acacagcgta ctgcatcgcg caccgccgca agtacagccc tgatttcgtc
     7081 ttctgccagc cactcaccgg catacgggaa agacacctgc gttccgtctg ccggaagtac
     7141 cggcagcgac tgccactgac tgccggacag aacaagttcc gccccggttc cggtcagttt
     7201 ctgaatggca gaggccaggt gcggcggcag tacgccgccg tgacggttga taagtaacgt
     7261 cgccagccgg ggcgcatcca gccgcacgta aatgctgatc cagcccggat ggttcggatt
     7321 tcgctggtca acatccgtct cttccggcca gccctgcgcc agcagcaggt tacgggcgtg
     7381 gcgacaaatc agtgcattca gcgtttcgtt catgattcat ggtctccctg aatcgccgcc
     7441 cgcaggcggc gataacatca gtcgttttcg cgtgtgttgt ttttcagcag ggcgcgggcc
     7501 tgattaacca ccgcctgtcg gcgggtgacg cattccggga cggcgaggcc gttatcacgg
     7561 gcggcccact cacgtttcca cagtgcctgt tcagcggtct gaatcaggga cgccagcagt
     7621 tcagcaggga cggtcaccag gcggttttcg ctctcgttgc ggataatctc catcaccgca
     7681 gcggaagaaa taccggactc aatccgctgg tcttccggga tgtcctccag gcgcgccagg
     7741 acggttctga tttcatgatc ggtaagggca atatccattc cgtcagcgag ggcctgaatc
     7801 tcttccgggg accagacaac cagcatcagc ggtgtgtttc ccggatactg cgctgccagc
     7861 tcacggcaga gcgtttcgca tgttccgtac attctcgtct ccttctcaat ctttgaagca
     7921 ggaatccagt tccagaattt cggtatacgc ctccataaac aggcgattgg cgcgatacag
     7981 cgtctggcgt gtcaggcgtt caacaagcac atcggaatca aagcgacagc gatgctccat
     8041 atcgttcagc ctggcccgaa ggtcacacat ggtgttggcc acctccgcca cgtcggcgcg
     8101 aagtttttcg tgtccggcat agttcattta cgcagcctcc ggttcaaacc agttatcatt
     8161 cgcggccccc tgctgcatgg cccgttgcac ggcggccagt cgttgctgtc cggcacggtg
     8221 atactgttca agcagctcga taccgatata ccggcgtccg gactggaggg cggccacaca
     8281 ggttgagccg ctgcctgcaa acgggtccag cacaatcgcg ttcgggtgtg tgaagctctc
     8341 aatgagcggt tgcaggctgg taacgggctt ttcagtcggg tgatgacgat tgcctgaata
     8401 tttccagccc tgcacgtcgg gcaatggctt ctgaggcagc gccggacgtc cttttgccag
     8461 gatgtaggcg cattcgtggc gatagcccac atatgcggcc ttcgatgtgt agtttttggt
     8521 gaagaccagg tgaccaacaa cgctgaatcc cgcatttttc caggcagaca taaagcgatc
     8581 gacgcgattc cagccataga agctcaccat taacgcgtct tttttgagta cgcggtacat
     8641 ctcattacag gccggttgca accattcgtc ggttttatcg ccggcaatgg tacgcccctg
     8701 acggtcacga aaaccgacga ggtacggcgg gtcggtgagg atgaaatcaa tggcgttatc
     8761 agggatacgg gccataacat cgatgcagtt accgaggaca aaacgggaca tacaaaactc
     8821 cttctcttac agagggcacc gggagggcac ccctgtggtg cccgttcccc gctgcctgtg
     8881 ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg gaataccgca gccgtcgtct
     8941 ttttgacggc aaggctattc gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac
     9001 gggctggcgt acactgccac cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc
     9061 cggccggacg cgggatactg accacaggca gcgttcctgc cgcgccgcgt ctgccggcgc
     9121 agtaaaaaga ggagcccggc gacatccttt gtcggaaaag aaaaaaggac gcaacagcgt
     9181 cctttaacag agttcagttc ttattcatca tccggaaaca agttcttaat gttgtttctt
     9241 gtttccagga gattctgatc gactggagca gatatttctt gcgtagcaga atcaaccgca
     9301 ggcaggatcg cttttgcatt attaccagca gactgcagaa atgcctccag caaacgaacg
     9361 acttcaccat tttcctgttg tggtagcgta ctactgataa ttcgttgaat atcctgcata
     9421 gacgtgctgt ctgtcaggaa ttcagctatc aggaacggca atcgttcatc ctgcttcatc
     9481 aatgcagcac cacacaacat catggcccgc attgcccgac cacgttcttt ctgaggcaat
     9541 ttttcaagaa acctgtcagc cacccgatca gctgcatttc gttccggata aatataaaaa
     9601 gagcacttac gattatcagc catactcatc ccttcattgc catcattccc aacaccagat
     9661 cgaactgagg attgtcactg acaaagaaac ggttatcacc cactcctgtc gcttttttta
     9721 ctgccccagc cacaaggctg gcaccaccgc caacaaccat aacatgagta aaaccagcaa
     9781 agcgtcctac tgaatccgcc acacgataat gcagtttttt ttcccggtca cgtaatactg
     9841 tcaggatccg ctcacgctgt tctgcattcg ggatataacg ggtcaggaat tcattgtcat
     9901 cccggccctg gattagacga tctgcaaaat aagaactggt tcgtgtactc acattcacat
     9961 ccatcgcatg cttaactgct tcagtaatga tggagacgcc agtcttgggg tcacaatagg
    10021 tttttgtgaa acctgacatt ttgctgcgga catgtgcaat atcaagggtc gttccaccga
    10081 tatcaacaat caacagcgac tccagatcat tcagatcttt cagcacgtca aaaccggcag
    10141 gcacggactc aggcaagaca ctgactttac gaatggtaaa gttttcacgc ccctgaactg
    10201 ttacagttcg ccggacatta tttttcttac gttcgatatt agccagattt ggctgatcgt
    10261 tctcatccag atactcacct aaaggtaaag tcacaacaat atcaacaggt tgtggagcaa
    10321 tacctgactg catcaacgca tgatgaatag caaccacatt aacgtcgctg tactgataac
    10381 gcgtttctgt ggtgcgcact gcatccggac tcaattgatc aaagctgtat ttttctccat
    10441 caatctcata gtttgcggaa gcgggtacag tggcatcagt cagtccctgc agcgtcatag
    10501 accattccgg cttaaaacta ttcggactaa ttaacgtttt cactgcaccg tcttcaaccc
    10561 aggccagttt gatgttggta gagccgtcgt ctacaaagat acgcatctag aacctcacat
    10621 aaataaaatg gattttttta gaaaaatacg agatcaaaaa cgattatcac ctaaaacaac
    10681 gtatcggtaa agacctgata tcggccacaa atggaaaaat aaagaaaaac acagacaaaa
    10741 accgacacca acagcacaaa agagaacaaa caagaacaaa taaagataaa cactgatgaa
    10801 atccagaaaa aactcaaagc ttcatataca atgtcagtaa ttcatcctct gacgccacca
    10861 ctacctccag ccaaaatgag cgggtaagat atccggagcc ttatagtcag gaggcttcta
    10921 caggaaagct gatgaggaag agaatgcgaa cgatcaccac acgtgaacag ttattagtga
    10981 acggaaaagt cagggaacgg attgccacac atatcgtgac aggcgctcac gggtatgaaa
    11041 cgctttgcac aagtggttat aacttacaat acaataagga acgggtactg attgaaaact
    11101 gcgaaaaggt ggctgatgga gaactgcctg tcacctgcca cacttgtttc tctatatggc
    11161 aggatgtaca ccggttcaaa cctggtgact ttgacacaga atcaggaaag ggaaatttca
    11221 ctgatacgga gctcacgaaa atcacaattg gacaagaaaa aacgccgaat gcgtgttgat
    11281 gtgccgcgtc agccggcgca gtaaaaagag gagcatggcg acatccttta ccggaaaaga
    11341 aacgggcggt tgcccgcccg tctccgtcat acttcatcca ccgtaatgcg ccaccttgtc
    11401 gactatccac gcaggtccgg tgacttccgg cagctcatca tcatctgtcg gggaagacca
    11461 ttccagctcc ccccacagca catcaacaag ctctccgcgc tcatacaact gccagccaca
    11521 gaaatcacag ccctgttcgg cataccagtg ctccagcgtg cagctgaaac ggcggcttaa
    11581 ttctgcaata acgtcgcttt ccggctgaca ccacggcgtg tcgaaatcaa cctgaatgaa
    11641 gttgtctccc tgactactga tattcaggtc atacccacaa ggccacttca cgccataccg
    11701 ttctgtatac cagtgatatg cagaaggaac accgttcaga acaccgccgt taaagccatt
    11761 cacttcaata tccaggcggg tcggcaaaac cgtcagcagg tcaaacggca tggttttttc
    11821 cggcagtacg ttgtcacaca gacggttcca ccacaccgat acatcctcgt taccccagat
    11881 gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg atagtttccc tgacatcatc
    11941 cggtaactct tcccagcgac gctgtccagt accgctctgc agccagagtt catgcagcat
    12001 caggcagttt tgctcatcca gcagtacacc gttctgcagg tgtgtcagcc agcgggtgaa
    12061 cgcgatattt tccggtgata cagcaccacg tccggcagcc gtcactccgg ggcactgctc
    12121 agagcggata ttttcagtga tctgcagaag tccggcactt ccggccagaa acagctgaat
    12181 accttcattt gtggcgcggc gataaagcgg tgtgactgca ccgctggcca gtcgtttaat
    12241 ctcagcgatc tgtgctggtt caccagaaaa atacatacga ttcgagcacc agtttggcat
    12301 gggaattctc ctctcacatg cggcctccat caggccgtgc cgcacctgta accgggcgac
    12361 cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt ggaggaggaa cgacgaaacg
    12421 tgaccgggtg aggaggccgg cgacagcgac acacttgcat cggatgcagc ccggttaacg
    12481 tgccggcacg gcctgggtaa ccaggtattt tattcacaga accgtgcgca aaatgctgtg
    12541 gataagctgg acacagcagt ccacagcagg catacagcca cactcccaga gaattgagcg
    12601 ttttttagtc acaataatga catttgactg tgtgtggatt cagttattag aatactgtat
    12661 atgcatacag caatagaaaa gggagatgag aacatgatcc gcattgaaat tcttttcgac
    12721 cgccagagca caaaaaacct caaatcaggc acacttcagg cgctacagaa tgaaatcgaa
    12781 caacgtctga aaccacacta cccggaaatc tggctgcgta tagaccaggg cagcgccccg
    12841 tctgtatctg tcacaggagc ccgcaacgac aaggataaag aacgtatcct gtctctgctg
    12901 gaagaaatct ggcaggacga cagctggctg cctgcagcat gagtaccgtt tatcaccgtc
    12961 cggctgaccc ttcaggcgat gacagttacg tgcgaccgtt gtttgccgat cgttgccagg
    13021 ccggttttcc ttcgcctgcc actgattatg ctgagcagga actggatctg aacagctatt
    13081 gcatcagcag acctgcagcc accttctttc tgcgcgccag cggtgaatcg atgaaccagg
    13141 ctggcgtgca gaatggtgat ctgctggtag tggacagggc cgaaaaacca caacacgggg
    13201 acatcgttat cgctgagatc gacggtgagt tcaccgtcaa acgactgctg ttgcgcccac
    13261 gcccggcact ggagccggtt tcagacagcc cggagttccg cacactgtat ccggaaaaca
    13321 tctgtatttt tggtgttgtc actcacgtga tacacaggac gcgggagtta cgctgatgtt
    13381 tgcactggct gatatcaaca gtttctacgc ctcatgtgaa aaagttttcc gcccggacct
    13441 tcgcaacgaa ccggtcatcg tactcagcaa taacgatggc tgtgtgatcg cgcgcagccc
    13501 ggaggcaaaa gcccttggca tcaaaatggg gcagccctgg tttcaggtga gacagatgcg
    13561 cctggagaag aaaatacatg tattttccag caattatgcg ctgtaccaca gcatgagcca
    13621 acgggtcatg gctgttctgg agtcgctttc tcccgcagtt gagccctact caattgatga
    13681 aatgtttatt gatttgcggg ggataaatca ttgcatctct ccggattttt ttggtcatca
    13741 gctcagggaa caggtaaaga gctggacagg actcaccatg ggggtgggca ttgcgcctac
    13801 aaaaacgctg gctaaaagtg cacagtgggc aacaaagcaa tggccacagt tttccggagt
    13861 ggtcgcgctg acggcagaaa accgtaatcg gaccttgaag ctactggggc tgcagccagt
    13921 tggtgaggtc tggggagtag gacgcagact gacggaaaag ctgaatgcgc tgggtattaa
    13981 cacagcactg cagctggcgc aggctaacac ggcattcatc cggaaaaact tcagcgtcat
    14041 tcttgagcgt acggtacgcg aactcaacgg cgagtcctgc atatccctgg aagaagcacc
    14101 accggcaaaa cagcagattg tctgtagtcg cagttttggt gaacgaatca cagacaaaga
    14161 tgccatgcac caggctgttg ttcagtatgc agagcgggcc gcagagaaac tacgtgggga
    14221 gcgtcagtat tgccggcagg tgacgacatt tgtacggaca tccccctttg cagtaaaaga
    14281 accctgttac agcaatgccg ctgtggaaaa gcttccattg cccacacagg acagccggga
    14341 cattattgcc gccgcatgca gagccttaaa ccatgtctgg cgtgaagggt accgctatat
    14401 gaaggcagga gtcatgctgg ctgatttcac accatcgggt atagcacagc cgggattatt
    14461 tgatgaaatc cagccccgta aaaacagtga aaagttaatg aaaacactcg atgaactgaa
    14521 ccagtcggga aaagggacag tgtggtttgc ggggcgagga accgcccctg agtggcaaat
    14581 gaaacgggaa atgctttctc ctgcgtatac caccagatgg acagatttgc cagtagccca
    14641 gttatgaata tgtaatccac ccataatatt gttatcttgt tatcttgtta tcttgttatc
    14701 ttgttatctt aattaatcta taaatcaaat acttaacagc ttgttaagtt gttaacataa
    14761 caaaaataat tgcatggttc gcgttcagat ggcaatatta agttgttaac ttaacagctt
    14821 aatatgaggc tataatgata atagtattag gtagtcaaaa gggtggggta ggtaaaagta
    14881 cactggcggt atctatagct gcgtatctga tgtcactagg caatagagtg attattgtcg
    14941 acgcagatga ccagaaatca gtattaacct ggtacaacaa tcgccctgaa gatctccctc
    15001 atattccggt tacaggcgcg acaggtaaca tcaaagccat gttaaaggaa cacgaaaaat
    15061 cctatgattt tgttattgca gactgtgctg gccgtgatag cgcagaaatg cgtagtggat
    15121 tgatggccgc agatgtattc atttctcctt tgcgcccatc tcagatggac ctggatgttg
    15181 tgcctcatac atgctccgtt tttacagcag caaaagattt taatgaggat gttcggggat
    15241 atctcgtttt aaacatgacg cccacaaata tgtttgttaa tgaagccaat gaggcagctg
    15301 aagtacttaa ggattaccct gaaatgcatc tggcaaactc ccgggtatgt gatcgtaaag
    15361 cacatcgaga tgcttgggcc gaatcaatga caatttttga aacgcaaaat gataaagccc
    15421 agcaagaaat cgaagcgtta gtgaaagagg ttattctgtg agaaatagag caaacacttc
    15481 acctcgtgac gaggcagcat ttattaacgg tggcacggct ggccttgata ataccaaaaa
    15541 gtcatcagat aaatcatcgg ttgtaagcaa agcaaaacca gtgagtattt ctttttttga
    15601 agaaaaccta aaagacattg atgcccttat aacggctgag atggtttcag gtaaaggcag
    15661 agtgaatcgt tctgatgttg tcagggcggc ggtagaagca cttaaaacac tatcaaaaac
    15721 agaggtatca cgattgatag cagaagtaaa acaaagataa caccttacta tcttgttatc
    15781 ttgttatctt gttatcttgt tatcttcaat gtattttagg atgacctatg ttgaatagaa
    15841 cctaaaacaa aacaaatatt aataggggtc cgcttggaaa agcggaaatt ttcccacgct
    15901 aagagaattg agttgaacga gtggttacat ccaggccagc taactcactg aaccccgtac
    15961 aggatcagac attcggtatc tccttcatcc tctgaggaaa acaaatgaat taactgctta
    16021 cgtatatgga tacatataca gtattaattt attaaggaga aaatggcatg tcaagattta
    16081 atgatagtga ttatcattaa catgaagtga tgttacaccg ttcagaaagc ctgctgacgc
    16141 tgtggcagca tcacagtctg agtattctct ggtgcaatac aaaaaccgga ctttcccgta
    16201 acaacagaca atgttacggg aaaagttaca gaactgagcc acaaaatatc tcagtacctg
    16261 agatgcataa aaacaccgta catatgtttc tcaaaagggg gatgatcgtt atccagccat
    16321 ttcggtggat cggtatgcag aacaccaatc tgcttcatgc cggtatgctg cctggatttt
    16381 ctcgcgcagt aatcactgtc ttcatatccc atcgtttcga tgaccactct ggccgtcttt
    16441 ccgtcaggag ccattgctgt gacaatgaaa tccggcagta catatttctt ttcaccatta
    16501 acataaactt ctgtatcaaa caggggcctt tccagactta attccggcgt acctggcttt
    16561 cttttaagcc agctggccac attcagaaga gcagtgagcg tgtttcgttc cagtttgctg
    16621 tccacaggaa ccggacatgt cagttgataa agagcatgag catacccttc actgcagatg
    16681 attctgccat cggcactccg gcaaaaagca agaataaccc agtaaggggg acgtattccg
    16741 ccctgcgctt caccattaat actgatcccc cgcacaggtc tgaagacctg aatattaccg
    16801 ttcgcccagt gaatttccac agcatcacga cagatgtgct gcgccataaa aatctgaaag
    16861 aacatatgtt ttcttctggc aggccagtgt cgttctgaat tctccagcgt ttccattaac
    16921 cgctcctgag acctttcaga cattcctggc cggaaatgga taatttcaga taaggatctt
    16981 ccccgaatat actcctgctg tagtgtaacc tgccgtatgg catcaagaca ctcacgataa
    17041 ttagtccgga tacggggatt agccagggaa tccagtttat tcagccctgc atcttcaata
    17101 agcgacagga gtaaacggcc aaggcgcgga cgtttcttgc gaacggagga gcgctcacca
    17161 ttatggtaag cagagcgggt ctgcagtctg ataacagaat tactgtcatc aggtggtaaa
    17221 aagtttctgt aatttatacg ggtgctaacg ggatgccggc ttgctccgaa ggatgtttcc
    17281 tcttcatttc ctgataactg acgatacatg gggcattcag gtgcatgctc atgattaaaa
    17341 ctggacagat aaagagtacc cgtattgttt ttaagagcag caaaattcat ggccggcgaa
    17401 tctcccggaa tacagtcaca ctgtaaccat gcatcatact cacgcatttc ccggagaaga
    17461 tcattggcca ggttatgttg tgccatggtt gctggctttt tcagtccaca gaccgtcttt
    17521 actgcagtct cttcctcact gctaagctcc cgaatcactt tgccccgggg attcttcttt
    17581 acaagtatca ttggcataat gaaagcactc cttatgtaca tgcttaaaca gtaagtaaaa
    17641 tatacaaaga acacaccatg aaacaaaggc agagatgaac tgtgcgcccc gatatcttct
    17701 tctgacatcc tttcaaatga atttttttta tttatatcat gacaacatta acgttagaga
    17761 atatatcaac catgtttcgg tcagggttca gtcgaatatc aattcgactg tcacgccata
    17821 actgttaccg cctcgagtat aaatattcag agaacggact tataaaccat gaaaaatcac
    17881 ctgacggaaa atttactaat ttatggattg tgatattatt aaagagtaaa accagccagc
    17941 cgaggtattt atggaaaaaa atcaggaaga aaggaaaatc cccgtcattg tagtgaaaaa
    18001 acgtcgtacc ttttcgttac cttccttatc cgaaaaaaca gatattatcg aatctgcatt
    18061 tactgaacag gcagcagaat cagcccccgt cggaataaat tcatctgccg tcgaaacaca
    18121 tatccccgaa gcaccagcca ggaagaagaa aaagaaaaaa cgccgtttcc cacgtccctc
    18181 ccactggacc agagagtata ctcacgagtg cgtggaaaaa ataaagatcc tgtttcctca
    18241 tcttcgtgct gaaggtggtg gatttattcc cctgaaaatt ggcataaaca atgatatttc
    18301 agcatttctg gcagaacacc cggagacaga actgaccatg gacgaatggc tctgtgcagt
    18361 gtcctgcatc acctcacgcc gtgtatatct gcagcggaca gctgtcgccg gcgtcccacg
    18421 ttatggtctg gatggtcacc ccaaagggca ggtctcagaa tctgaagcac agagtgccgg
    18481 tcgcagactg gtgacattag aacaaaagtt gctcaggatg caggcacagc aggagaatat
    18541 ttctgggcaa taaacactcc tgataacagc tggacattac gtatcagaaa aatgggaaga
    18601 gaattattat gagtaaatca ggtgcagaaa aaattcaatt catcctgaat aatcatgaac
    18661 aggcatacag acgcctgaag cctgtatata cttttttaga actggcatct tggatttcgg
    18721 ttgttctgct gattatatac ctatcaggca agaatcacgt gcttacagca gcttttctct
    18781 gctttgcatg gacagcattc tgggttcttt tccttttaca ttatttccca aaagttttct
    18841 ctattctcga tcgtcagagt ctgctcaacc tcatggaggc caccaaagac tcaccagatg
    18901 taaagcagga gctgcttaac cgcctttttt ccggaaaaag aatgaccggg cgggatgaaa
    18961 tggatatctg ccgtcttctg agaaaaaatg aagaagaaaa aatccgtcag agtgaactga
    19021 atatcatcag gaattttatt gatgaacagc agttaacgaa tagtgatatt ccgaagaaaa
    19081 gtacggtccc aaatgaagat aaaaaaggca taagatgata tgaaattaat aaccaggcaa
    19141 gcagaggatg gcgtggtttc atcctaagat tgataggggt taacctgtaa gataaaagga
    19201 ccgtgtttac acgacgtctc tgaagggctt cgtttgggaa acggacaata tctaactgac
    19261 gaggaaatta aataagggtt taaaaattaa cgcaccatat attatacaca gtatataacg
    19321 attcagatat cgtcaccagg atatgccgcg tcagcggcat ggaaggcggc actctgttgt
    19381 tgcatatgac acaggagtaa aaccgccgaa gcccggcgta agccggtacg gactgataga
    19441 tttcacctta cccatcccca gccctgccag accatacccg ttttcagcca tgagagagct
    19501 tctgtgcgcg gtcggagtgg tcccgacgag ggtttacccg aagtcggggc gtatctccgc
    19561 gttagcgggc cgtcagggcc gcttacgagc gtgtacggag gacttccagc cagaagactg
    19621 gcagcgatga taatgtagtt acaacattca taattaaaag cgatgttgtt cccgcccttt
    19681 gggcgggggc ggcctcgctt ttcagttatg aggggtaggc acggtgctgc gtttgtgcgg
    19741 tgtttttatt cctgatgtct ttttgttgtg gtttcagaac ggtgaggttc ggggcccgcg
    19801 cgtcagcgtg ggaggtgctt ttagattctg agcggcgcgg ccttggccgc tgcaagcgaa
    19861 ggcctctctg tatttgttca cagtggtttc agagataaag actttccctg cggcgaagca
    19921 acgcagggga ttaaataatg ggggtaggac tatattgcag gtttgctgtt tcaggtttgc
    19981 agctttttaa agttctttgt ttgcggattt atgtggataa ctgaaacaac gtggccatgt
    20041 tctgatgact gatacgtggt tttatggaca gatatgcccc tctgagggga tttaaggtga
    20101 ttactgcaga ttttgggctg agcggaacca gcgccgaaag ccccggaggg gctggcgatg
    20161 attcagtgct gaaggaatgg tgctgtctag tgcggcggtg gcgcagccag cgtcagttca
    20221 agctgacgca actccctgat ggccagtcgc ttcagatggt catcggaaca aaaataggct
    20281 tcatccggcg gcagacgctt cctgagatac tctgccatct catgaatctg ctggtcggca
    20341 ggcagtttct tcagacgtct ggcgcgctta ctggctgccg cttttgcacg acggtgtttc
    20401 agtgcatcct ggctgcgctg cagataccag cgttttctgg ccgcatgtac ggagatatct
    20461 tcatcctcac gcagtacgcc ttcccggatg agttgctggc ggatttcact ttcacgcaac
    20521 cgcttctgct gctgttcgtg aagtttcacc atgtcgacgc ccagcatctg ccagcctgcc
    20581 ggtgttatcc agacgtaacg tggcagacgc tggcgggttt cacggtccca cagggtttcc
    20641 tctgaaacac ccagcacacc aaaacgtacc tgttcggcca gcaaacggga aaggcgggag
    20701 accgtcactt ccagttccgg aataaccttc cccttgctgt ctttcgggct gatttctctg
    20761 gccagacggg aaacactcat gcctacggta tgtgtgcccg catcactgaa gctgaccaga
    20821 accggccaga tggcatcgag gaggcgttgt ttttccggac ggaaagcata tttgcgaccg
    20881 gcatacgttc gtttctgcac gtaaaaagga tgcagagaca tgtgcatgcg caggctctgc
    20941 tcgccggtca ctgggtcctt tttcaccaga cggttgtagg cgtgcccgag ctgcccgctc
    21001 agcgccttat agagctcagg acgataccag gacggatttt cgcatttggg tttggtggag
    21061 tgctcaccgc gacggcgctt cgtgcgcttc ctttccggtt caaccaggaa agtggtcgcc
    21121 cttcccgctt catagtcttc ccagaaacgg atctgctctt cgggtgctaa ctgactccag
    21181 tgtactgcgt tatatggagt attcttcaga cctgccacaa gctgtcgctt atggcggagc
    21241 gattgtgccg tgtatttata cactgcacag ggttgaatct ctggtacggt ttcattatac
    21301 tttacccgta ttctgtgagg cccccataat ctttctccgt cccgccaagt tcgtcgaaag
    21361 attgtggggg ttttcctttt atggcttccg gaacatcttc ggtcttctga cttactgccc
    21421 gtaaatgcca tctctcctga atgccttatg gcgcattctg ccgtccggct ttcgccgctt
    21481 tgctccacag agtgccaccc tctgcgcagc taataacaac cgaaatatac ccgctttacg
    21541 ttaatttgaa aagcattaca acgatctctt ctcttccagt gcctcctcac cgatacctct
    21601 aatgagttaa tcttgacctg cgtcaaaaaa ataaaatcta tttttaaaag cagatttttc
    21661 tgctttagat ccttcttttg gattaaaaga tcgacttttg ttctactcac gatcatttat
    21721 cttacaaccg atcaacctaa cagaataata ttctgtttta agacaattaa acgatctaaa
    21781 aaacctttaa aaaatcattt tgtagatcat tcgatcgtaa atagaatgat tcttagataa
    21841 taaaaccatt tgttacacag cccgccaatt gtgtaacaac agcctctaca agagcatatt
    21901 tgttcatatg ttcgtcagaa ctgcggattg tctggacgag ggtcagacac cgtttcgtgc
    21961 atatggacca gcatgttcga acgccacaaa gcagaagcat catgcctgga tcgcttcggc
    22021 gcggagcgtt aagttttgac gcttttttgt atgtttttta agcggttagt tgactcggta
    22081 cgttatacta agtacccttt actggtcaca ggcaaaactg tgatcgggcg taggaacccg
    22141 cgtagtcatc gtcggcgcag aagttaattt cttcatttgg cgtcgttttt tgccatacca
    22201 taaagacagg aacttgtatt tcttgtcgtc atctatggta gctcaggcgg ggcaggcatt
    22261 agcctggccg gtttccgatg acgccggtat tcctaccccc gtctgggcta tcaccaatga
    22321 gcgtaggaac tcggtgatag cgttttttgt tgtcatcgga gacctttgct atgtcacctc
    22381 aaatgataca gaaactcccg gccatcactc tgttggaagg catgtttccc gagctatcaa
    22441 caaaccagct taaagtctgc gttttttacg caatgggtgt tccttacgac gccattgctc
    22501 agaactgtcg gctttctccc gaaacggtac gaacctacct gaagcgaagc ctgaagaact
    22561 taaatctgga aggatatgat gcacttcgct ctgctgtgct gatgcgaacg ttcgttttca
    22621 tgattagcaa tacagctaaa gaaaacgaaa aaatgtgaac aggtgtcccc attaaagggg
    22681 acactttcat ggaaattgca ttttgcactt tacaggcgct cccaaattga taaacataaa
    22741 caccaagcac aagttacttt aataaagcaa atcacttcag acggtgtccc aattatttgt
    22801 atctggctca tgcccgatat atggcgacct tactctgaac tgacgtgctg atgttttatt
    22861 tcgcgtatca cgatactgtg ccactgtatt gctgatatta gtgcttagcc gttctttatc
    22921 tgtgagttcg ccaaatcacc gatacagaac gtactagagt ctgctggtta tatgcctgtc
    22981 agaactgcag tttatccaga tatgggctgg acactgcttc agacatatga tttagcatat
    23041 tcgcctgaca tcgcaaaatc gtccatcatg gatcggtttt gcgcggagcg ttgaacttcg
    23101 tgaatataga acacctgaat aaccggaact ggtatcttgc gcaatataat acagccggca
    23161 aaaacagaga atctttattc tcatggctga atgagcaaaa cgtcgtacca tggactccat
    23221 taataacaag aaagataaga cgggcagact ccaggtgctg tcaccgtgaa agaatattcg
    23281 ctatttttcc agggtacttt tttattctgg ctaattttga cattcaacca gtatcggcat
    23341 tacgtcgcca tagcgccttc attgatttcg tgaagtttgg cggtgaaata aaacccgtca
    23401 ataggtaatg actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt
    23461 gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg
    23521 ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg
    23581 cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac
    23641 gtcaaagggt gacagcaggc tcataagacg ccccagtgtc gccatagtgc gttcaccgaa
    23701 tacgtgcgca acaaccgtct tccggagcct gtcatacgcg taaaacagcc agcgctggcg
    23761 cgatttagcc ccgacgtatc cccactgttc gtccatttcc gcgcagacga tgacgtcact
    23821 gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaaatg gcggaaaatc
    23881 gtgttgaggc caacgcccat aatgcgggcg gttgcccggc atccaacgcc attcatggcc
    23941 atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc
    24001 catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct tttgccgtta
    24061 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
    24121 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccttcaaacg
    24181 aaaggtcttt atttgctgtc tgtattctga agagtccaag gaatcaaact tgaacaacaa
    24241 aaataggtta tatgaaagca tcatcatttg aaacacggct tcattcgccc aaaatgactt
    24301 tagcaagaga tgacccaccg ccatgtcgta tttggcttct ttgatatagt tttcagcatt
    24361 accacgcttt tcatagtata taactacttt ttcagaaagc aaggtagtat ttgttacaaa
    24421 gaaaaagtag tcgtattcgg aaccttctaa aagtgataat tgtgctcttt ctttttctgg
    24481 tttcagtacg cgagatacga caaatcttct gtctttttcc catttaacta attttgtata
    24541 cagttctgta gtttctctac cttcttctcc tttaacgaat acaattgatg aattcgttgc
    24601 ttgtgaggtg agtgtagaat aacttttggc tttaattaaa tatttgcatc caagagattc
    24661 tatcgtttcg ataatttttt catcaaagta gccactatcc attcgaaata aaatttctaa
    24721 atcgtctgat ttgatgttag caacaatttc tttgatcatt tccgcagcac cgtttgcagt
    24781 gtaagtattg ccacttctta caaatccggt aacatatgct tttaattcgt cgcaaaatgc
    24841 aaattggata ttgtagcatc ggtttcccag tttcttagga ttatatcctt ttgacgcacc
    24901 ttcttgatga ccttctacgt taattacact actatcaata tcaatcgtaa tggatgtcaa
    24961 tttactttta gtgagcagtt ttttaaagac tttaaaatta atgtctctaa acatttgggt
    25021 tgtcttgaag ttgaagtttc ctagaaaccg tgacactgtt tcaggttctt ttacggaaat
    25081 atcaaactcg ttgacgaggg gatcattttg aagtagcttt agacgttcta acttatcaat
    25141 gccaatgaag tgaccgcaga gcatggtctt tatatgattc atcttgattt tatttgttga
    25201 gtcattatca aatacgaggt cattttcaat aaaatcaaaa atcccattgc tttttgcatt
    25261 ctcaaggagc agaaaaagac ctgcatttga tgttagattc ttagctttga aatcaatttt
    25321 attaatcata attagaaccc ctttttacta cttttcttac tattatttta ccatatatcg
    25381 agtcataaaa gctgataatt taacatattt ttgagcactt ttctttcacc caatgggtga
    25441 aagctgaatt tcgaaggaat gcatatttat caaggctttg attatgcttt ttgaagtact
    25501 gacgtagaat ctaggttatc aacataggtg ttagccacat cactgatgat gtcctcgctt
    25561 aggcgataaa ttttctttgt atagcgccag tatataaaac acaaaatact gtgtggtgac
    25621 gataattcgt ttccatgaaa taaagattct agtttagaag ccagttcttc atttttttgt
    25681 tggctctgga gacgactcat tagtatgcgt tcttcatctt ttctgtcgat aaataataat
    25741 ggattgtcgc tgcgtttttt atttcggcat gcaaagattt gctcttcaag cccagggatt
    25801 gtgttgatga ctttttttat ctttttcagg aaaataggtt gttttaactg cgacatttcc
    25861 atctcctaag gggattcttt tattaactaa aatcagcata acatgcgttg ttgtgaagta
    25921 gagaaccatg tattgggttg gttgttaact tcaaaaacaa cgctgaatca ttcaaataat
    25981 aaaaggattg ttacagatac gaaagtcgtt agaatagctg cgggcgcttg aggctgtatg
    26041 ccgaaagcgt tttgtggacg gtatacagca gaaagcccct ggagattttt ttatcaatca
    26101 accaagggct ctactgtaat gcctcaacaa cattatagta gcccgataac cgccggaagg
    26161 caatggaggg gctatgatgc cacagcgaac gtttttaatg atgttaatcg tcgtctgtgt
    26221 gacgattctg tgttttgtct ggatggtgag ggattcgctt tgcggactcc ggctccagca
    26281 gggaaacaca gtgcttgtgg caacgttagc ctacgaagtt aaacgttaac gggcaacacg
    26341 gcggcaggtt ttctgccgcc gctttgttgc gaaggctgac ctcattgcgc cctttcttct
    26401 tcaatgaagc gtatctggta ttgtttcatt ttccctgaag ccgattagct ccagccgaat
    26461 gcctttgcca tcgggggcca cgttctgcat ttcgcagata ccacgtcggg tcatgagtgt
    26521 gaaggctgtc attgccagca tggctaacgc gttttcctcg cttccgctgc gtttctcgag
    26581 agcatcggca atttcttcta tgctccccac ctgaaagcaa gagtcgaatt ctttactcca
    26641 cttttctgac atcggatttt ctctctgtat ttatgggtag ttatcgaact gaatgtgcca
    26701 gttttttcag gagacggcaa agttgagatg attggttctg attcaaagca tgagtttccc
    26761 cggtgtgacg ggcatgtcgg gcggattttg agaaacaaca gatgtaaaaa aaccgactgg
    26821 tgaggtcggt ttttttactg ttccgttaca gagtgggtgg ctctggtcgg aaaagaactg
    26881 tctataactg acttgtagat agtacgtctg gaattatatt tctgcaagta ctggataagt
    26941 gtatactcgg catgtcagct ctgaggcatg ttctgtccga catattgttc agcgggaaga
    27001 acaggttgca agtcctggcc aaaacatcag gtggtgctgg ttttggtgct ctggtcggag
    27061 aagaactgtc tataactgac actatagaaa ccgttgtgac aggcagtgta agagcgaaca
    27121 acggggttct ttctccagtg atggagaagc ggatgattaa tctgttaatc atcgttctca
    27181 gggcagtagt tgccgttgca aacgcgctga ttgctgttct ggaactgatc cgggaactta
    27241 tcgactgata agcctggaaa cgtaactaag ggcagggagt taccgcttcc tgccctttaa
    27301 caaaaagaga tctcacgtta aaaatttgta cccttcttta acttccagtg cctgctcctt
    27361 tgactcaaac atcagtcttg ttttcccggg aacaccccat gaaatgtatt caacatctat
    27421 ccaccatttt ccatattctt catatggctc ggatattact tttttgacaa aggcgtctac
    27481 cagattcatt gttttttcct ttttgtgttg aaaataatta atgccagagc acagcgaaaa
    27541 taatgaatgc gctggctgtg ataattgcga ttttctccca tatgatattt ttctcatggg
    27601 ggtttctacg gagattattt cttcgcattg agataccggt attcatgttt tatcttggat
    27661 ttttgttttg ttgtctgtct ggcaggctgt tctgcgcccg tttgtgacaa ggttagctta
    27721 tggggttaaa tggtcacgga catcatgcgg acggttccct gtcacatgcc atctggtttt
    27781 ttcattcaaa ttgagagata ttgctaagtt gagtcgcttg cgatagtgga ctcctgtttg
    27841 caagtgagtg ccattgttta tttcatgaag aggattgatt atgagcatgc ctcctgcgat
    27901 agctaatact ttcttgtttg aaatgatgaa gagtaaatca aaagacgtta cacttgctgc
    27961 gatctatgca ttaggtgaag gccggtgtca ggcggagaac attactcgtg aattacatag
    28021 attgagtcaa agtgatgata tggaaataaa aattgctgcg attaaagcac ttggtcgaat
    28081 ttatcggtaa cggcataagt tatctgttgt aatgaacaaa agccggctca tttgaaccgg
    28141 ctgttatgga gggagcttat cttaaaatac ttcggctaca ggtatgcctg caatctgctc
    28201 ccactcctta cgcttttcct gaatcaggcg aggaaagtcg acagggtaat catatcctcg
    28261 acttgccatc gcgtgcatgg tgtttgatgc ggtttctgga tgagtacacc atgatggaat
    28321 aggaaccatt ggcaggttga tgtatttcgt atgaatttcc agtgttttgt cgtctgttac
    28381 agtacgagtg aaattatgaa tgtcaaaacc gataacatcc ataatgttgc ctggataaat
    28441 acggacatac aacattgtgc catttttaag gtcaataccg aaataagctc cctgagttaa
    28501 agataatcct tcatacccct tctctggaga gtagaagcct gactctttta ttgtttgtac
    28561 aacttttctt attttttctc tccttgtttt tgcacgtgag tacatccata tgcctggcgc
    28621 aagtaataag aaccaactac caaaagtgac aacataatct tcgaccttat ttccgaaata
    28681 acgatcagaa catagcattt cggtaaagcc taatgcgcag aacagaaaaa aaggtattcc
    28741 aagaaagaaa taagcttttc ttaatacata ataaaatagc caccaggttg gaaatctgtc
    28801 tgttgtttct tttttagctt tcatataatg cctcccctcg tactatctat tattttaatg
    28861 ccgatgggga ggttgtgcaa tatttatatt gatattatcc ttttattccg tccttgtcgt
    28921 cacttccttt tgtattatct ttaagacgat ctgttttcat tccaggagta tgtcggatat
    28981 tgcgacttcc tccaataaat atttctttaa cagaatttgc catgctgcca agcgtgttgg
    29041 ctccatcacg tccccctagg aagttaataa catgatcagg taggtaggcc tgaagggcaa
    29101 atatggcagc tacagtggtt gtacaaattc tggcgtatat cagcaagaaa ccagcaagac
    29161 tgaaaatacc ggtcagcgaa ttgaactgta cgttaaccag ggcagcgcca aataatgcat
    29221 tcagtattgt gcctacggcg atgatggcta cagaagcaaa gaaaaaacca aagaccatga
    29281 tgggcggcct tatcatacta tcaattaagt atatataacc gtaggctgcc cgacttcctc
    29341 tgtcttctga tgtcccgaga tgtgttgcag cccacagcgg cccggcggta catcctatta
    29401 atacgctgac tatccagttc ccgatgcctg tcatccagaa aataaacgga atgaatggca
    29461 ggtaaattga taaggaaaat ccagcacaga acatcaggaa caaaagaaaa taaacaggtg
    29521 gtgcaagtgc ttcaaacagc gcggtaagca tcgaagcggc acctgttagt gagttaaaga
    29581 attttacaag aattgaatct ttgccaccgg caacgacaac tctggccgtt gtatatatag
    29641 tccagattcc ttctgcactt accattgtta tatctccgat atttttcatt tttatcagag
    29701 gattaatttg agcactggtt gtgcctgtgc cacttgtttc cgttgcaatc ctgttagtga
    29761 gactaacaag ggggcccata atttcagaca aggcagattt cggatcttgt gcatttgcca
    29821 tgcgttgttc actggatgat actactgtgc ccagcggtgg cgtataggag gaattctgca
    29881 gttgcgtcct ataggcactc atgaccgcac tgaataagtc agtatcacca acttctccca
    29941 gtgatgacat tgaagttaca gctggagctc tgtcagccag ctcggccaga cgctggttag
    30001 cagtggcaaa ggtctgatac catgcgccaa gcgttaccca cccgtaggtt gtgagataag
    30061 atttaagagc ctcttttctg gattgctctc cgttatcagt gggtagcgat ttttgtacag
    30121 cacgttcata ctcgtcggct gctctctgaa tccgtgtctc gatatccggc aatgttccgt
    30181 tgccagaatt tcttttttcc agaaatgttg tcacaaactc actggcagcg ttgtccatgt
    30241 cgctaatcat attatccatg gctgcacgtt gtgctgagat aacaccgctg tattcgtttt
    30301 tactgaaggg attaaagaat tttccaatcg tcgattgatc tgttgttccg tttccttcta
    30361 ctgacaggct ggctgaacca cagataccgc tgccattgga taccgtgaca gtgtaattac
    30421 cactggcggt tttagctgat tctgtcatca gtgaagtcga ggatttggcc gtctgattaa
    30481 aatcatttag tcctgcgtta accgcatact tacacagttc catttcaaaa atgccacgcg
    30541 ctgctgttcg tgtggatgcc tgaactggct gtacagtcat tgaataacca tttgcgatat
    30601 tatcagcggc aagttgcacc atcacattgg cggacccaac ccccattaac cgccacatca
    30661 gaggcagcac cgtagttacg cggaacagga gtatgctgag aaggggagtt gcagccagcc
    30721 agaaaaaggg cggcaagaca ccccgcaggt ataatacggt tcataataat tcctgagcag
    30781 ttaaatcaga atgggatata ccggtgctcc ggtacagaca ggatgatgtt tgtttccggc
    30841 tatacttgcg ttaccggaaa taaatgccag tgtggccagg taaatagcct catagtttac
    30901 tgaaggtgat tattcctgat actggctggt tgatgtgtcg tcagacataa aacgaatatg
    30961 agcgtaatct ttaacgctcc gcgccgaatc gatccatgat ggatgactcg acgatttatg
    31021 aatcgaacgt accggcgctt gtccctgaaa cagttcccgg ccctcgtccg gataacctgc
    31081 aattcagaca ggcacatgtg cccgtacact cttgtatctt actgagtata tcagaattta
    31141 tgtaaattca aatatcctct atgcctcttt gttgtatttt ccgtcaagta tgagccgggt
    31201 accatcgtaa tgcatgatca gggcatcaag gtacgccggt ttgaatttct tgccgagcct
    31261 atctctggcc agaaatgata aacaatgatt atgtgtcatt attactatgt tccttttatg
    31321 agattctctt gcgattgatt caagcgtctt ataaagatca ttattgcagt ctgataaact
    31381 gtccataacg acgggttctt taccggagaa gaattttgca gtttgtatgg ttcttaccgc
    31441 attgctggag taaatatcat attcactgaa gattgttgcg aacttaatac cttcctgctg
    31501 tgctttctct gtaccggtaa tagtgactcc gcttttatcc gaatagcatg gcatatctga
    31561 acggtcgcaa cgttctccat ggcggaaaag gaaaataacg gtgttatcag cattaatttc
    31621 ttttgcctgt tccagggtga tatcgggaag cgagcttttt gcttttgtca ctgccgctac
    31681 caggaaaaca gaaaataaaa gaatgaatgc tgtttttgaa taagaagaac gtggtttcat
    31741 catgttattt aaactgaata taaaaatacc cccgggttta gtgccggggg cggggacagt
    31801 aatcagaaag tgaactgaag ttgtgccatt gcaccgtaat cgtggtcagt tcctaccagg
    31861 ccggaaacat cgatatgtac gacgtcaaat ggagagatac caacgccagc ggtgaaggcg
    31921 cttccggagt ttgatgccat gttctgacga taaccggcac gcaattgcgc ccatttccat
    31981 gcgttaaatt cagcaccaac acttgcaaac tgacgtttgc tgtcagaagt gaagccactg
    32041 gcaggagtca gatcaatgtc aagagcagtg gtaaacaggt cattatgcca ggatacaccg
    32101 gcggttgcct gcggacgaac tttgaaggtt tctttaaagc cattgacgac tttagtatca
    32161 atactgcgtg gaatgatgtt ctgggcaaca agaccgacag tccagttgtc attcaggtct
    32221 gtataagcac caatatcggc gttgaatccg tttttcgtgt tgtggtactt gtcaccgtca
    32281 aaatcgtctt tgtcgtaatc acgaaccgtt acgttgtagt taaacaggtc cacacgctga
    32341 tattttggtg tgacgccaag agaccatttt tgtccggccg tttccagttc tttggcaaat
    32401 gaaattccga cgtcggtaat tacagctgca cgcccgaatg cgcgtgaggt cagggcattc
    32461 ttatcgacgt cggtaattgt tccattggca actttgtcca gataatccag gtctgcatcg
    32521 tttacttttc cgtttacgct gacagttccg taagacttga ccataagggc aaacggcaga
    32581 gtgtcatttg ccatggctgc tacagctgat acacctacct gagcatctgc attgatattg
    32641 cggaattcct gcagacgatg tttaagattt gctgctgcct gttgtacgcc atgctggtta
    32701 tctacagcac tgtcaaacaa atcccagtca tctttaacat catcagcttt atttgataca
    32761 tcatcaggat cagcaacttg tgccccaaca gacggcagaa gaaggctgaa atcatcattg
    32821 ctgttgtgct ttgtcagtaa agccgggttt gccagaggtg ccacaccata atgggaagat
    32881 gctaccccgg tgccacccat tgcatcgtta cgtgcttcaa aatatgacgt cgctgctgca
    32941 gcgttaaagg aaaataatac agcagatgca atgatggtgc gggtaatatg atttttcttc
    33001 atcatttaat ttcctgttta tcagaattta gacaaaacaa cgctatgaca cggatgtgcc
    33061 gtaaattttt actttggtac tatctggtat ttaccagggt aataaagacg tattaaataa
    33121 gctaacaggt agtatttgcc ctgtaataag gttcccggca aggccaggtc tcaacgtaat
    33181 gcgtcgcaaa gacattgttg tattctgcct ggttgtatat tcagttaacc attttctttt
    33241 ttcatcgttc agaagtgttg gtgataatga tagtgccgga tatattacag agccatttat
    33301 ttcttttttc tgtctcaata ttaccccgca atattctctt ttcgtgtttc ctgcgaaggg
    33361 gaaactatgc cagaaaataa cttcagtgtt gctgctatca ttaataaaca ttctgaaatt
    33421 atcagcagta tctggccatt caccggacaa cgctatcgga tagttttcat ggccattcag
    33481 ccagtccgga ttgagattaa aacattcagc aagatgttcg agagcgtcag ctgaaagata
    33541 gtctgcaagt ctggccgggt cctgtataac cgtggagcgg atattccatg gcgacagcaa
    33601 tgttgccagt aatggcgcat ttatatcatg cgacagcatg atgtattcaa ttctgcttat
    33661 tatgtttcct gtggtgttat ccgcaggcat aaacatatta tgaagagcat cctcaagcag
    33721 gattcgggtt aaatcagcgg tggatatggc taattgttcg ctcattatcc ccagaacttc
    33781 tcttacccct tgggggtaac ggacctgcag tggtgtcggg tttatctgac gaccgttgat
    33841 gagtttttcg ccgattgctt ttttgtccag cgagagcatc attcgggccg caataccggt
    33901 cgtattcagt ctcattgttc ctccgactga aaaagtcttt tcagctcatg tggttattgt
    33961 atcttcggtg ttttttctgg tgaaacgaat aatttcgatc atgcagatcg aatgtgtttg
    34021 gcgagttgtt gcaactcgcc tttctgtatg tgttttgaat gatgtttaca gcggggtgct
    34081 gcccgggttc agagggcaaa tcataatgta gtcagggtcg ttaacaggta acgctccggg
    34141 agatgggaca cgttcgcctt tctgtgctgc aatggccagt gtacgcaaca aaacctcact
    34201 ggcgcggtga actgcttcct ctttcgttgt ggcagtgacg tgaaggttat caaaatcacc
    34261 gatctcaata cgatgcgctt tctgcccatt ttcttcatcg atggtcgtga tatgggcagg
    34321 atacggcaca aaccatgctg ccactttttg tgtctgccgc cggcgggcat caagcttact
    34381 gaccagttgc catgcacgaa gatgagtata ccgtttcagc atattcatag aacgatgtcc
    34441 tgatattgca gcaatctcca ttacattcag gctacccagt tcgaagaagc ggcttattgc
    34501 ttcatgccgt agatcgtgaa aatgcaggtc ctcgatgcga agtcgttgtg tggctattct
    34561 ccaggcattt ttaaagccgg atgcggtgta atcaaaaaca ttgccgtgga gattaacggg
    34621 catcatctga agaaagttac gggcacgtct ggacagagga acatcccgtg agtgaccgtt
    34681 tttggtttca ggtaaatgag ccacaccgtg gcgcaaatca atgtgctccc aacgtaaggc
    34741 cagtatttcg ccctgccgca tggctgtttc aagggcaaga tggaaaatga catacaacat
    34801 cagatttttt tcgcggaaat agcgagaaag gcgacgttct tctgaagacg ttagccggcg
    34861 atctcgtccg gaggatactt tcggcttgcg aaccagttca accgggttag tacgacaggt
    34921 tccccattca acacgagcaa tattgaacag agatgacaga agggcgagtt caagacgtac
    34981 agtattacct gtaatggctt tacccgttcg ggggttgatt tctgctaaac gaacgtctct
    35041 gtatgtagca atatcaacgg ttgttatttc gtccatattc cgaagggcaa tgggatatcg
    35101 cttgataaca ttgctccggt aaaactcctg ttgatgccct ttcttgtgaa cagaaactgt
    35161 tttcaggtac ttatccagtg cgcgtgacag ggacatttta cggatgcgtg gagaaggcat
    35221 gctaacctca aaagaggcaa aaaataatgc caacaatcat tctctgcaag cgtgccaatc
    35281 cggt
//
