LOCUS       NZ_CP029116            25348 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR435 plasmid unnamed3.
ACCESSION   NZ_CP029116
VERSION     NZ_CP029116.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291528
            Assembly: GCF_003073835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25348)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 14.36x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003073835.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:07:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 4,858
            CDSs (with protein)               :: 4,858
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 107 of 294
            Pseudo Genes (incomplete)         :: 192 of 294
            Pseudo Genes (internal stop)      :: 63 of 294
            Pseudo Genes (multiple problems)  :: 61 of 294
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029116.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR435"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
     gene            complement(<1..1273)
                     /locus_tag="CSC22_RS25625"
                     /old_locus_tag="CSC22_5370"
     CDS             complement(<1..1273)
                     /locus_tag="CSC22_RS25625"
                     /old_locus_tag="CSC22_5370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_249933983.1"
                     /translation="MGNLMKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGWQ
                     AEARLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQ
                     RLQAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALR
                     SWSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITRN
                     SKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERTAV
                     AGASCSPNGLVGRDNTGAILSCQSGTWRKSNSGSTVITGRIANGQQIPLPTGFSASQCS
                     WSVSNAENPQGWKPNYFAGSVATYD"
                     /name="shufflon system plasmid conjugative transfer pilus
                     tip adhesin PilV"
     gene            complement(1261..1917)
                     /locus_tag="CSC22_RS25630"
     CDS             complement(1261..1917)
                     /locus_tag="CSC22_RS25630"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="WP_001401940.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWYK
                     THTAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFMLQMAVTDALTGLLPGTFTRR
                     FLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITAWSG
                     LYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI"
                     /name="A24 family peptidase"
     gene            complement(1902..2462)
                     /locus_tag="CSC22_RS25635"
     CDS             complement(1902..2462)
                     /locus_tag="CSC22_RS25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000014005.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAAA
                     GARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIKMG
                     VIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYANKV
                     WRIYRDMKGICLPGQGGRQCRQS"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(2472..3086)
                     /locus_tag="CSC22_RS25640"
                     /old_locus_tag="CSC22_5371"
     CDS             complement(2472..3086)
                     /locus_tag="CSC22_RS25640"
                     /old_locus_tag="CSC22_5371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="WP_000908227.1"
                     /translation="MLIQNIDVNALKKNEKETSHDKGWGIMEQGAIALVVIIVLALVFG
                     GLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGTKMTGALIQMGGVPKTMTV
                     RGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTGLTN
                     GITLNSTTHNDGKVTTEEASAQCTADNGSTGTNKLIFTING"
                     /name="type 4 pilus major pilin"
     gene            complement(3103..4188)
                     /locus_tag="CSC22_RS25645"
                     /old_locus_tag="CSC22_5372"
     CDS             complement(3103..4188)
                     /locus_tag="CSC22_RS25645"
                     /old_locus_tag="CSC22_5372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="WP_001208802.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTAL
                     EQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGIRS
                     GSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKISSP
                     DSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWSIYKD
                     IQGAAFLLNMAALLKAKMTTLNSLNVLQDFASPWLSTRLDSIIYRVRQGDHLGLALRQC
                     GYQFPSREAANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLIFLVMSL
                     LLLVMAVVDIQSIGDNSMGNL"
                     /name="type II secretion system F family protein"
     gene            complement(4201..5754)
                     /locus_tag="CSC22_RS25650"
                     /old_locus_tag="CSC22_5373"
     CDS             complement(4201..5754)
                     /locus_tag="CSC22_RS25650"
                     /old_locus_tag="CSC22_5373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_023518854.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLNLYP
                     DIIPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGASDI
                     HFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQDARL
                     SPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLNVMLR
                     RPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTPIICDK
                     SDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTLHTNSAL
                     GIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSEDDRAYLER
                     HCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEVIEPDNRLF
                     QILKTRGKVAARKYWLENMHGISRVEHLLRRINDGLVDPLEADRIIPLDEDERLSIDDA
                     "
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(5765..6217)
                     /gene="pilP"
                     /locus_tag="CSC22_RS25655"
                     /old_locus_tag="CSC22_5374"
     CDS             complement(5765..6217)
                     /gene="pilP"
                     /locus_tag="CSC22_RS25655"
                     /old_locus_tag="CSC22_5374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="WP_001247337.1"
                     /translation="MRPGKSLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQAK
                     VQGAQLQKQLEESDVNLSSETVSGFSGMTASLPSVSEQPTSKNRKELPVIMEINGKDKR
                     LHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
                     /name="pilP"
     gene            complement(6204..7499)
                     /gene="pilO2"
                     /locus_tag="CSC22_RS25660"
                     /pseudo=""
     CDS             complement(6204..7499)
                     /gene="pilO2"
                     /locus_tag="CSC22_RS25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /name="pilO2"
     gene            complement(7492..9174)
                     /locus_tag="CSC22_RS25665"
                     /old_locus_tag="CSC22_5377"
     CDS             complement(7492..9174)
                     /locus_tag="CSC22_RS25665"
                     /old_locus_tag="CSC22_5377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA];
                     GO:0009306 - protein secretion [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="WP_000748143.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSAH
                     AREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQELG
                     QRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTSTQP
                     LTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSSSSSV
                     SSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGRYWLSA
                     SSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDWNLVYKS
                     LHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDVSVVTSQE
                     STVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLLPLIQKTGN
                     LQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLVVTGFDQNNT
                     TTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
                     /name="PilN family type IVB pilus formation outer membrane
                     protein"
     gene            complement(9188..9625)
                     /gene="pilM"
                     /locus_tag="CSC22_RS25670"
                     /old_locus_tag="CSC22_5378"
     CDS             complement(9188..9625)
                     /gene="pilM"
                     /locus_tag="CSC22_RS25670"
                     /old_locus_tag="CSC22_5378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="WP_000539806.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQILM
                     IANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETLRL
                     KSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
                     /name="pilM"
     gene            complement(9625..10692)
                     /gene="pilL"
                     /locus_tag="CSC22_RS25675"
                     /old_locus_tag="CSC22_5379"
     CDS             complement(9625..10692)
                     /gene="pilL"
                     /locus_tag="CSC22_RS25675"
                     /old_locus_tag="CSC22_5379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="WP_001360287.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISESA
                     GVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKPGS
                     TPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLNRLL
                     EEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESSTTANSQNKPRN
                     PFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLRENIIKWA
                     EETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKPLYAQAS
                     RMQCIITVSDTRDGR"
                     /name="pilL"
     gene            complement(11021..11614)
                     /locus_tag="CSC22_RS29495"
     CDS             complement(11021..11614)
                     /locus_tag="CSC22_RS29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001330804.1"
                     /translation="MKNNNAPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYFC
                     VVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMRKH
                     WDVLLSLISKAINNDEIIVMHSHLFTPARINKLIKRLESYDLSFSVITYPRKTSLYEKL
                     SIPFIYLLFQWSFPHLHSDGMTVVIRPLQTHHTN"
                     /name="hypothetical protein"
     gene            complement(11739..11993)
                     /locus_tag="CSC22_RS25690"
                     /old_locus_tag="CSC22_5380"
     CDS             complement(11739..11993)
                     /locus_tag="CSC22_RS25690"
                     /old_locus_tag="CSC22_5380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001136187.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNCL
                     VVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(12165..12848)
                     /locus_tag="CSC22_RS25695"
                     /old_locus_tag="CSC22_5381"
     CDS             complement(12165..12848)
                     /locus_tag="CSC22_RS25695"
                     /old_locus_tag="CSC22_5381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="WP_023518856.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSGA
                     SRHPEFSSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKLQK
                     AGWRIYVCPWPEMVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITPLHR
                     KTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGATQEQT
                     TITLH"
                     /name="conjugal transfer protein TraC"
     gene            complement(13102..>13341)
                     /locus_tag="CSC22_RS29085"
                     /old_locus_tag="CSC22_5382"
                     /pseudo=""
     CDS             complement(13102..>13341)
                     /locus_tag="CSC22_RS29085"
                     /old_locus_tag="CSC22_5382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /name="transcription termination factor NusG"
     gene            13396..14093
                     /locus_tag="CSC22_RS25715"
                     /old_locus_tag="CSC22_5384"
     CDS             join(13396..13645,13645..14093)
                     /locus_tag="CSC22_RS25715"
                     /old_locus_tag="CSC22_5384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            <14109..14222
                     /locus_tag="CSC22_RS29090"
                     /pseudo=""
     CDS             <14109..14222
                     /locus_tag="CSC22_RS29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            14546..15691
                     /locus_tag="CSC22_RS25720"
                     /old_locus_tag="CSC22_5386"
     CDS             14546..15691
                     /locus_tag="CSC22_RS25720"
                     /old_locus_tag="CSC22_5386"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class C beta-lactamase CMY-42"
                     /protein_id="WP_039023136.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQAI
                     PGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGEIK
                     LSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQPQWT
                     PGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKDYAWG
                     YREGKPVHSSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQSRYWR
                     IGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVHKTGSTG
                     GFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
                     /name="extended-spectrum class C beta-lactamase CMY-42"
     gene            15785..16318
                     /locus_tag="CSC22_RS25725"
                     /old_locus_tag="CSC22_5387"
     CDS             15785..16318
                     /locus_tag="CSC22_RS25725"
                     /old_locus_tag="CSC22_5387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="WP_001221666.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWYE
                     IARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRAAL
                     KVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIATREG
                     FEVNKLIWVKQPGA"
                     /name="lipocalin family protein"
     gene            complement(16315..16632)
                     /gene="sugE"
                     /locus_tag="CSC22_RS25730"
                     /old_locus_tag="CSC22_5388"
     CDS             complement(16315..16632)
                     /gene="sugE"
                     /locus_tag="CSC22_RS25730"
                     /old_locus_tag="CSC22_5388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_000118520.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIAM
                     LSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKLST
                     H"
                     /name="sugE"
     gene            16828..17610
                     /gene="finQ"
                     /locus_tag="CSC22_RS25735"
                     /old_locus_tag="CSC22_5389"
     CDS             16828..17610
                     /gene="finQ"
                     /locus_tag="CSC22_RS25735"
                     /old_locus_tag="CSC22_5389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023063753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FinQ"
                     /protein_id="WP_023063753.1"
                     /translation="MMKGFLKPESRDNIPAQILKELPSWSIYVSAENLHTILPTSYPIH
                     GFFFYPFLDNNGNIIRLFIIDDLKQSQGTTGLKEKNVDVVNNIIRIKDSREGLLDSRKM
                     ECIDGEVVVTVNEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHV
                     HTHHELPAQNIREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGS
                     KKPELAHERKPRLIWLPPVPVNLEDVNKLPVVITPIDK"
                     /name="finQ"
     gene            17917..19125
                     /gene="trbA"
                     /locus_tag="CSC22_RS25740"
                     /old_locus_tag="CSC22_5390"
     CDS             17917..19125
                     /gene="trbA"
                     /locus_tag="CSC22_RS25740"
                     /old_locus_tag="CSC22_5390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="WP_011264049.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLIL
                     HTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVTLW
                     CLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLEDGDPEKLFHGGKRPERRVA
                     LTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQYFLD
                     DRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFKRVIKSDGAQQWLKQHRYVR
                     SGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARAEAEAMR
                     FGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDILPRTPATD
                     NEF"
                     /name="trbA"
     gene            19144..20214
                     /gene="trbB"
                     /locus_tag="CSC22_RS25745"
                     /old_locus_tag="CSC22_5391"
     CDS             19144..20214
                     /gene="trbB"
                     /locus_tag="CSC22_RS25745"
                     /old_locus_tag="CSC22_5391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="WP_011264050.1"
                     /translation="MTIEYFATRIAKHISVQAIWPGGRTEIIAVLPQDALSGLVTRNNQ
                     LIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRAIIAS
                     VVLFVMAMIAVSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLPVPP
                     KKDVQVPEKTLTSKNPEAAAARHNLAAVLKRNADRGMFTVNLSSGHERTLYAFLDPACP
                     NCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRLYSADN
                     GMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTGILQETG
                     TLNLFLKTTDSESGHE"
                     /name="trbB"
     gene            20207..22498
                     /gene="trbC"
                     /locus_tag="CSC22_RS25750"
                     /old_locus_tag="CSC22_5392"
     CDS             20207..22498
                     /gene="trbC"
                     /locus_tag="CSC22_RS25750"
                     /old_locus_tag="CSC22_5392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="WP_011264051.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWP
                     LALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEV
                     ILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCW
                     ARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSN
                     TWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQ
                     MLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQ
                     HAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSAL
                     GRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEAT
                     QVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGE
                     EARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFSIFRGETVPSA
                     SFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTA
                     KPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTT
                     TREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKK
                     NNRPDWDGMY"
                     /name="trbC"
     gene            complement(22536..25235)
                     /gene="nikB"
                     /locus_tag="CSC22_RS25755"
                     /old_locus_tag="CSC22_5393"
     CDS             complement(22536..25235)
                     /gene="nikB"
                     /locus_tag="CSC22_RS25755"
                     /old_locus_tag="CSC22_5393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="WP_011264053.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQP
                     HRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIA
                     QQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDN
                     LHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKT
                     AVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKF
                     LVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAAD
                     VRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVI
                     CDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPA
                     TDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELF
                     AQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRV
                     QPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRKPDLRY
                     GERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIA
                     DGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTP
                     VYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVL
                     FGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHHREGSERYYR
                     DYIAANSNDDASLPPPEQDKRWEPPSPG"
                     /name="nikB"
     gene            complement(25246..>25348)
                     /gene="nikA"
                     /locus_tag="CSC22_RS28370"
                     /pseudo=""
     CDS             complement(25246..>25348)
                     /gene="nikA"
                     /locus_tag="CSC22_RS28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /name="nikA"
     ARG             14547..15688
                     /name="blaCMY-42"
                     /gene="blaCMY-42"
                     /gene_name="extended-spectrum class C beta-lactamase
                     CMY-42"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(22543..25235)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.321"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          20208..22465
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.805"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 catcataagt tgcaactgat cccgcaaagt aattcggctt ccagccttga gggttttctg
       61 cattgctcac actccatgag cactgactgg cagaaaatcc tgttggcagc ggtatttgct
      121 gaccatttgc tatacgacca gttatcaccg tactaccaga attgctttta cgccaagtac
      181 cggattggca cgaaagtatt gcccctgtat tatcgcggcc tacaaggccg ttaggcgaac
      241 atgatgcgcc agcaacggca gttctttcca gttgtaagta ttcaccagta taaaggcgac
      301 catcagcccg aacagtaccg cctttcactt gaccgccggt atagatgccc ttgttgttta
      361 cacttcgaac ccatgatcca tcggacatat aaaatccgcc accgtgagtt tcattgagcc
      421 agcctttgct gttacgagta attaaccaac cattattact tctgatatca ccgccagcgg
      481 tgacatttac gtcaaagttt ccgctattgc cttttacctg accgctgaaa gtgccattta
      541 caccattcac attgccgctg aaattaccag tctgggcatt tactgcccca acgttattca
      601 ggttatttga tcccatatca atggccgtgt gcattttgtt taagtcaggg cgaccattga
      661 cctggaatct gtaaagacga tcggtgtctt cagctgcgcc actaagttca tctgtcgata
      721 acaatacggc aatatgtcca ttaccacttt tggcaccata attacttaaa gctactgacc
      781 aggaacgtaa tgcacctgtg gctgttttgc cgtcctggat gtatccacca agaccagtgg
      841 taatatcctt agccatctgt ataagtgctt tcactggata aggtgtgcca ccactggata
      901 cgaccattgc ctgtagtaat tccgggtttt gggtgtttcg aactacatat gcctgtaacc
      961 gctgcccctc gctgtttgtc tcagtaaagc cgctggacaa aaagccggta tttttcagca
     1021 tggttgtcgt gataacggca ggggttgttg tggtactact ggcctggaga gttgtgtagt
     1081 ttttccctat ataagagcgg gctgcactgg cccagttgct gacaagacga gcttcagcct
     1141 gccaaccttt ggtttttaaa tagtcctgcc atatgcctgc accccaggca actatgatca
     1201 taacaatcaa taaagccgcg ccaatttcca gcgatgccca gcctcggtca tattttttca
     1261 tcagattacc catagtggtt gataaagatt atccagtagc aaaaggacat ggccaagaca
     1321 cagccatgga cccagtgggc cttctttatg tccttttatg caccatgttg aatgccataa
     1381 gacaaatcct cctgcgccca gcaacacact ccatatggcg ttatatagcc ctgaccatgc
     1441 ggtaatacct gcaataagcc acaggtctcc tgtgccaata ttaagacgat ggcggttaac
     1501 cagtttatgc agacagaata agacaattgc ggcagagaca atttctgttg cacggagcca
     1561 ccatatatca gttgtgattt gtgataatat cccagcgatt aaaaaacgcc tggtgaatgt
     1621 acccggtaag aggcctgtta gtgcatccgt tactgccatt tgcagcatga acgaaagaat
     1681 aagaacagca tgaatacggt agagaagtgg ggatgacgat aaaataatta atgccacagc
     1741 tatactatat agccatattc ctgacagaac tgcagtgtgt gtcttatacc agagatgttc
     1801 tgtatcagat aaaaaataac gaactctgaa taacagagtc cgtccaataa tggaaaatat
     1861 gaacatcaat gggaggatat aaacagggga gagacttatg cctacgattg tctgcattgt
     1921 ctgcctccct gcccgggaag gcatatccct ttcatgtcac gataaattct ccatacttta
     1981 ttagcatatt gctctcttgt ttcatgtctg tccttacgga atccggcatt gtatgaccca
     2041 aggcaattcc agctgacgcc acatatctga aaatgtcttg ccagtatcca gcttcctata
     2101 tggatattaa gacatggttt ggtaattaaa tcctcagact ttttgattac gcccatcttt
     2161 ataagctttg gaatatgggt tgagtttatt tgcatcaacc cataatccgt actactggct
     2221 ttgcctgttt ttctgtcctt atttatgtta attgcgcccg gcttcaatga agattccccg
     2281 gcagaaatgg ccttcaacaa taatggttca atttgatagc gtgcgccggc ggcggcaaag
     2341 caattgtccc acttgttagt ccagatttgt ttgggggcga tgtatgaagc tcgggcggga
     2401 taaataagag aacaaagcag gattaatgaa ataagtctga ggggcacttt acccccggac
     2461 attaaaactc cttagccatt gatggtgaag attagtttgt ttgtaccggt gctgccatta
     2521 tcagctgtgc attgcgcact ggcttcttca gtagtgactt ttccatcatt gtgagtcgta
     2581 ctgttcaggg taataccgtt agttaaacca gtacgactga tttgtgtggc aatctggata
     2641 caggcatcct gtgggacttt ttcataggtg acggtaaaac cattgttgaa acctgatgtt
     2701 gaagccggag caacagttac ggcaccgccc catgagttat agagcgttgc tgtacctgat
     2761 gatggagtac cacgcactgt catagtcttg ggtacgccac ccatctgaat gagagcaccc
     2821 gtcattttgg ttccactggt aaacgtatat ccatcgctac cttttagcag cccctgagta
     2881 cttgtgatga ttgtctgtat gttcgaactt tcatttgcca caccggttcg tgaacgcaac
     2941 atatacaatc caccaaaaac aagggcaagt acgataatta caacaagtgc tattgcaccc
     3001 tgttccataa taccccagcc tttgtcatga gacgtttctt tctcattttt cttcagagcg
     3061 ttaacatcaa tattttggat taacataaat acctcgtttt atttaaagat tacccatact
     3121 gttgtcgcca atagactgaa tgtctacaac ggccatgacc agtaacagga gtgacatgac
     3181 aagaaatatc agcataatta aacgcacaac agctgctcgt ttttttactc gctccagcgt
     3241 ttgtacaagc caccgttggc cataattgcc gattagctcc gttgcgccat ctccctgtaa
     3301 taaagacagg aagttggctg cttccctgga tgggaactga taaccgcact gacgcaaagc
     3361 cagcccaagg tgatccccct ggcgtacgcg gtaaataatg ctgtcaaggc gtgtgcttaa
     3421 ccatggtgat gcaaagtcct gaagaacgtt cagtgagttg agcgttgtca ttttggcttt
     3481 cagaagtgca gccatattca ggaggaatgc tgccccctgt atatccttat aaatactcca
     3541 tggcattata ttgtcagcca aagagcggct tttccagttt ttcagagacc atgatattac
     3601 acctgtgacc actgcaaaaa gaacggcaca tatagcgccg taattatcga caaacaaaga
     3661 taatccatag agaaaaccaa gagcaccact ccatgaatca ggggagctta ttttgctcag
     3721 tacaggtatt agctctgtat taagaacata taatgtcccc gtcatcatga ttaacaaccc
     3781 caccgggtaa atagccattt gccatatggc ctgatggagt tgctttttgg catctgtaag
     3841 tgtggtggca aattgtaatg catccacaat ggaaccactt cttattccgg cactgattac
     3901 tgcagcctct tcctggggaa cccataaacc aagagtatat tccagagagt tttcgccact
     3961 gttttcacga agagactcaa tacagtctgt ggccagttca gcaaagggat gccattttcg
     4021 tccaaagtct gtccaggcat cccgcatctg ttccagtgcg gtctttaatg gctgtttatt
     4081 ttccagcaga aaacgcagag cttcgtaaaa ctgcaccctg tagggggcgc tgaacgtttt
     4141 tctgacaatg aaacgtctca gccgttgact gaaattcatt tcacgcatgt tgttctcctg
     4201 ttaggcatca tcgatgctga ggcgttcgtc ttcgtccaga gggatgattc tgtcggcttc
     4261 aagagggtcc accaggccgt cattgatacg gcgaagaaga tgctctacac gactgatgcc
     4321 atgcatgttt tcaagccagt atttacgtgc tgcaactttg ccgcgtgttt tcagaatctg
     4381 aaataagcga ttatcaggtt ctatcacttc agcaattacg gtcctgccgg tgaggccttt
     4441 acctatttca ccgcgctttc tgccgttaat aattacgtcg tgattacagt cggagcatcc
     4501 gtgaggattt cggaaccaga tattatctgt actgcagagt gaatctttat tgcagtgccg
     4561 ctcaaggtaa gcgcggtcgt cttcgctaag ttcaggtact cgcttttccc atggaatacg
     4621 gcaggaggga cacagagttg gtacaagacg ctggctgatc atcccaatca gtaattgcgc
     4681 atcgcaaata agatcggcat tcatacccat cgtaatcatc cgctccggta tacccagagc
     4741 tgagtttgta tgtaatgtcg tcagaacgat gtgaccggtc tgagccgcgt aggttgttga
     4801 catcatcgag acaagatcgc gcatttcccc ttccatgatg gcatcggggt caagtcgcat
     4861 tgccgatgaa atagcacgac tccatgccag tttgacggcc tcttcgtcgg atttgtcaca
     4921 gataatcggg gtttgtgttg cccccagaat ctgtccttcc agcggatctt cgatcgtcag
     4981 caggtgtcgc ccctgattat cgtcaagata tacacggcag gcactgcgta acgttgttga
     5041 tttacctgaa cctgttggac cagacagaac tattttccct tccgggcggc gcagcatgac
     5101 gttcagcaac cggatttgct ccggtataaa gcccagttgc ttaaacgtgg gaactttgtc
     5161 tccgtcatcg ggtatcaggc gcataactgc aatcagacca tctcccgtag gtctgtgact
     5221 gtatcgtgca ccaaatattc ctatttttcg cattaattgt ggggacaggc gcgcatcctg
     5281 ttccctttga gggaagaaac tggtttcagt cacatcagcc attgacagaa tggctgttgc
     5341 gcaaagtgaa taacccaacg caggttgatc ttcatctaca gtttgcagtt caccgaatat
     5401 tctcatgcga actttgaaaa tagactctga aattaaaaaa tgaatatctg aagcaccaag
     5461 ttttctggcc gtttcaaaat aggagagaac tttcttctca ctttcactga catctgcaag
     5521 atctttcaga tgaataatcc gttctttcgg acctttattt tcatcatcct gaccggcttc
     5581 ctgtaactca gacagcgaga ctacttttgg gatgatgtca gggtataagt ttagcagttc
     5641 ctggagatta ctctgtacgt caggtcttgt tcgctgattt gtttcgatga gaatttcatg
     5701 ggtgtctccc cgggatataa aaaggagagc atccttcaaa ttatatttgt ccatatcaca
     5761 tcactcagaa agtaagggtg gtgccatcac ttaacgtgac gccagacaat gagatcgact
     5821 tcaccgtgac ggatgtaccc ggaagctggc taccagtggt gacgcttgtc tgacgtccat
     5881 cagccatgcg aagaacggca tgaagacgtt tatctttgcc gtttatttcc ataataacgg
     5941 gcaattcctt tctgttttta gatgtcggct gctctgaaac tgatggcaaa gaggcagtca
     6001 taccagagaa acctgaaaca gtttcagatg acagattaac atctgattct tcgagttgtt
     6061 tctgtagctg tgcgccctga acttttgcct gtaaaagaat attccggttt tgttgtgctt
     6121 caagttctcc tattgtgaca agaggttgtg ttgttgcaaa tgaaaaaccg gagaagaaaa
     6181 atatggatgg aataatgagt gatttacctg gacgcataaa tatgaccttc tgaggaataa
     6241 ctgaaagtac cgccgtttaa ttcaaaatgg atattgctca gacgaacgcc ggtatcctga
     6301 aataaaggga acaactcatc aggatttaca ggcgttgata aactgaactg atattctttc
     6361 catttctgaa caggggctgg ttcaccattg ttacctggta atggttccgg tatggctatt
     6421 tcattaatgg ccggcgttaa ctgttttttc tgaaaccacg tgaacacccg catcagttgt
     6481 tctgaggggg cagggactgc ctcatccctg cgaggcaggg aaggcagtag ccgggttaca
     6541 gaagccaggc gtgcaccatc tttaaggtta aagtcaggaa taacattaaa gatttctttg
     6601 cttcttgcca gaaagccttc aatagttcct cctggctgtc gttcgtaaat cagtgtgaat
     6661 gtctcgggag tacatgttcc accggtaagt ttccagcctt ctagcgcgac cggtgatggt
     6721 tttcgaaggt ccgcgcaggc tttcagaaaa tcactgatta caggttgtga cgcccatgga
     6781 tgcgggagct acggtggtgg ttcgggcttt ttaaactgta atcttgcccg tgcagctatc
     6841 tcctcggcgg tcggtccgac atcaggctct ggctgagtca tccagaatac tgtgcctgcg
     6901 ccggctgcta ctaaaaacag tacggcaggc agtgtgaatt tgctgcgatt gccgacagta
     6961 agtttgcaat ggcggaggtc tgctgatgat aaccgggtaa taatactttc ccagttatca
     7021 ggatgttcca gagacgatac aacctgccat ttttcggggg gctcttcgtt catcgccaga
     7081 aaaaggctga ccttttgggc aacatcggca tcatttcctg ataagtcagc cataacggca
     7141 ggctggccat taatggaagc aaggaataag agctccttat cgctgtaccg gaagacaccg
     7201 tatccatttc tgacccatga gcagaaagcc agtgccaggg aaaaataatg attgcgttct
     7261 ggcctggtca gtattcctgt gccggtaagg cggctgatct ttccacggga atttctgctg
     7321 cctacagtca ctttcaggga ggtaagccgt ctgggtttta catgatactt gtgtttatga
     7381 tgtgagggga cgcgctgttt ttttacgaca ggagtccatt taagacaggc agcccacacg
     7441 cgatgattta ctttaggatc tgcgagaagc actaccgggt tgatatcttc atcagccatt
     7501 attgcctcct gacgggaaag tcggggtaat gatgatcaca agcgtgctgc gttcattttt
     7561 cccggtttgt gaaccaccaa ataatggatt tgctggcgta aatgtaccgg ctttacttgt
     7621 cgtcgtattg ttctgatcga aaccagtaac gacaagtgat tgcccttctt tcagattgac
     7681 cttctggctc agtgaacgca gtttggtata tggcatttcg atgtaactgt ttccgtcttt
     7741 tgacgtaaag ctacggatag ttgggggatc agacagatta aagttcatct gcaactggag
     7801 attgcctgtt ttctgaatta aaggcagcag ggtcatattg aatccagtgg tgatcatgcc
     7861 cggtgttaat gttgttgttg ctcctacatc cgtagtcgta gttgttgctg actgggcgac
     7921 gtaaactgtc tgatctgcca tctggatagg taccggcgtc aggtttgtga cagtgctttc
     7981 ttgtgaagtt acaacgctga catcgccttg ttcactcagt gctttaatca gaaggctgga
     8041 accgctgaat ttggcggcat tccctgttgc cgtatccaga attgatacgc ctgcagatgt
     8101 agcgcctgta aaatctccgc ttgcattgtt caacgttgct ccggcggaat gtagcgattt
     8161 ataaacaagg ttccagtcca gaccgaactg ttcgtttctg gtattgctga cgctcagaac
     8221 ctgtacgttc agggctacct ggcggttcat aatactgttt tgttcgtcta catatcgtgc
     8281 gacggcttcc tggacagctg gagtatcagt gactgtcagc gttgagctcg atgcagataa
     8341 ccagtaacgg cctttttctg gtgtcagcat tgcttcaata gttttccgga tatcttcata
     8401 cagatcgtat tcctgaccaa cggttgtgct ctgagaggac gttgcatcac cggatgctga
     8461 gttatcctgg cctcctgttg cccccattgt gcttgttgag ccagagctga cactggaact
     8521 gctgctggtt ttggtgttca gcatatgaag tggatacgtt ctggtttcag tcagatagaa
     8581 tacaatccga ccattatcca tgcgccagta cagaccactc cggctggcca tcagatccag
     8641 aagaccattg atatctccct gccacatgag gttattcagc gttaatggct gagtggaggt
     8701 agtcattgtt gtgctgccca ggctgcttaa cggtagacgt ccattttcat ctggtgctgg
     8761 tagtgttcct gtcatctggc gggtagcgcc tccttcaaga gttgaattgg ctgcgtcagg
     8821 cgtgatgatc acagggatgc cgcatacggc agtaatacgt tgccccagtt cctgcagagt
     8881 gatctctcct tttcttgcct gcgtgatgta gcaggctgga gctgtttgtt ttttctctct
     8941 tgatacctga gcgaccggaa cagggtttat ccattgatta tcgagccagg taagagcctg
     9001 cgatttacgg gccgaaaggg ctgatacctt ttcccgtgca tgtgctgagt cttcctgtgc
     9061 ttttttctgc attttgttga tttcgctgaa agtacatccg gagatggaaa gagcgacggc
     9121 gatcatgcag gggagcaccg ccagcttcat tgaacgctgg tgtgattttt tcatgaatat
     9181 atccagatta gttaacgtag acgacagaac cagcagtaat gcctgccggg ggtgtcagac
     9241 cggttgcagt tcctgatagc caggtgagtt gcccgttttg gaagatccct attagtgcag
     9301 aaccacgact ttttaaacgc aaagtttcaa caagtcctgg ttgttcaggc atccataccc
     9361 acagacgccc ctgttgtaac tgatgtttaa ttcgggagtc cggagtgaca gggagagcca
     9421 gtttatctga ggaaattact ccgtcctgct ggcctgatac gtatctgata tcattgattc
     9481 tgttagcaat cattagtatc tgacttgccc atacagacgc acttgcatat tgctgggcgt
     9541 ttgtggtttc agacatcttc tgattctggt agctaccagt gataatcaga atggtcaggc
     9601 cgacggccat gaccagccat cccattagcg accatccctt gtatcagaaa ctgtaatgat
     9661 gcactgcatg cgagaagcct gtgcgtataa aggtttttgc gcagtccgat ataactcaaa
     9721 tacctgctcc atggctgatt caaatgatcc ccggaacatc aaaggcgcat caagacggta
     9781 atcggttaca gaaagtggcc atatgaccat ccagtgcgtt gatgccatgc tttcgcattt
     9841 tgtttcttct gcccacttaa taatgttttc ccttaatgta gtgcctgccg gcgcacgcca
     9901 ctctttaccc tgagataccg gtttgacagg tgtcccggtc atgagtggga tagacttgac
     9961 tgtagagccg gtcggagtcg ggattgctgc gggcgtagac ggagatacgc tgtttcccct
    10021 gaatgggttt cgtggtttgt tctggctatt tgctgtcgtt gaagactccg gggaagtgga
    10081 tgtggttacc atggctgctg gcgagatagt ggttgccgtg tcctgtgccg gtgcagcagc
    10141 cgatgttgtg acggtcaacg ttttattatc ccagtccaga tggccccaga ggtgttgttc
    10201 ttccagcaac cggttcagag aacgggtcca ctgatcattg gcagaccatg aaaccagttt
    10261 ggtattcagt ttgggcgtgg ctgcattctc ttgtctgaat ttccatcctt ccggtagtaa
    10321 tgtttttatc cactgactga cggtccggtt acgggccgtc ggcgatagtg ttacaggggt
    10381 gctaccaggc ttgcctgtaa tgattaaccg ggagaaattc tgacttattg ttggtgaagg
    10441 taacggtcgg gattcgctta ctggctgtgc tgtttttact gtctctggca cgctgactct
    10501 cgggggcgga gttattttag gtggagaaag tcgttgttgc gtaagtgcca ggacgccagc
    10561 actctcactg atttgtccgt caacaaaaac cagagcaggc gtatctgatg gtgccacgtt
    10621 attgccgccg gaaattccgg cacagccgga caataatgac ggcagtataa gtgtcagata
    10681 gttttttttc ataacggaga ctccgtgttc gtatatacgt gtttgttagt taatgcggac
    10741 tttaagtaag ggttcattat tttatgtgca attggatggg taatgtatac cggtattgag
    10801 agaattactc ctgaatgtcc cgttttagtc gcattcagaa gtctgcttgg gtctttaata
    10861 ttgttgatta gtattttcat tattctattt tttgaatttt aatgttgcat gttattttaa
    10921 aggtgaggtt gtggatagcc aattaagcca gtgttttggg attttttgtg aaaataaaaa
    10981 tcctgcttat gagcattctt tttatatcta aatgtatttt ttaattggtg tggtgggtct
    11041 ggagaggacg aatgacgact gtcattccgt ccgaatgcaa atgcgggaaa gaccactgaa
    11101 acaagagata tataaaagga attgataatt tttcgtacag acttgttttc cgtgggtatg
    11161 taataactga aaaactcaaa tcataagatt ccagtctctt tataagttta tttattcttg
    11221 ctggagtaaa gagatgtgaa tgcatgacaa tgatttcatc attattaatt gcttttgata
    11281 tcagagaaag caatacatcc cagtgtttcc tcattgactt cgggcaacgt gtgtcagcat
    11341 aggggctaag atgtatagtt gatttcttgc ttcccttttt cttcttcaca aagaatgtga
    11401 agtcgtattt agatgcagac cttacaataa gaagtacaca tctgaaaagt agtgggaaaa
    11461 tgcaaatcac aaccaccaca cagaaatata acatataagg caatgataag ttgtcagtgt
    11521 atggataacg taagtattgc catggggtag tgatgtacag acaaagcgta caccaaagca
    11581 accagagata taatggtgcg ttattatttt tcatgttggc tgaggcaggc gactaaaata
    11641 acagactaag gaggattaac tgtttaattc tttggaacaa aaaaatgcag ttcctgatca
    11701 tcacacacca ttgaataaac attttgcttc ggttgcgttc atgatagcca gtcctgtaga
    11761 tgctgatggc gggagctgct gcccttgtgc cagcggcagt ggctgccggg agcacgctcc
    11821 agtcggaaca gaaatcggtt gtcgcgtagt accaccacga gacagttatc cggcgtgcag
    11881 aaacgtcggg caagctcatc ggggtctgtc ggtgttttcg ttgaaaacag cctgcgtttg
    11941 cagtgtgcgt cgacgaccag tatttgcagt cgtccgggat gttgctgtgg catcaaagac
    12001 tccttccgga acgggaaaag aagcagctgt accgtcataa ctgcacgtac cggttgcgaa
    12061 aaaacggcac aagcgagtag tgacagaaag aatgtcggaa acggcaggaa gggcggtaca
    12121 ggctgcgaag actggaaacg gggtatgccg gggattacac atcctcagtg taatgtgata
    12181 gtcgtctgct cctgtgttgc accccagaca tcttctggca agctgggcag cccgataaat
    12241 agggtgtgaa agagggtttc tctgcgttcg atcccgaatt gatgttcgta tcctctcacc
    12301 acctgaaatc cttcgtcgga gaatattaac tctgccgttt ccagaagctg catagttttg
    12361 cggtgtagtg gagtgatcag ctgcaacgta actggtgccc tcatatcgcc tgtcggtaga
    12421 aaacactctt cggtgcgact tagttgtagc agatggcgtt tcccggaaca ggcccgttca
    12481 atggattcat tgaacaccat ctcaggccat gggcagacat aaatgcgcca gcctgctttc
    12541 tgtaatttac ccagcgcagc ccgaaaacga aacaacggac attccccgac agtggcagag
    12601 ctgagaaaga gcgtattcag cgttccgatg atgggaatat tgcgatcttt ttcggtcaat
    12661 gcttttaacg ccatgatttc gccagggaga ctactgaact caggatgtct gctggcacca
    12721 gaaagccatt caagcaggta atgccgtaat ccggtttcac tggttatatt gtggttttcc
    12781 tgtgccgttt tcagggcatt cagtgcacac cagagcaact ggcgcacggg atgttctgtc
    12841 gtggtcatgc gaatgtccta ctgtgagatg aaaagaagac cggtatccgt tctctccgcc
    12901 acacgtgatg gcacgggccg gcctttgata aaacgaatca atactctggg gtgaagcctt
    12961 ttatttcttt cgccattaag aaatacacgg ttccatcaaa agacaggggg agtccatggt
    13021 ggggatggct tcgtgcaggc ttcactccgg aatattctct tgaattgaaa aaagcccggc
    13081 ccgaaggccg ggaaatactg gctatgggat gtttacaagg cgctccataa agccgtgatg
    13141 ttcggcattt gataccagtt ccaggagcag ggatatcctc gactcaggct ggagagtatt
    13201 ttccattctg aggagatact gatattgtgc ttttgtcagc catatgctgg aagccgcatt
    13261 cagctcttcc ctggctcccg gatttagcac agggtccggg taaatcttca ttaatccatc
    13321 aacaatatct ttattgacgg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
    13381 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
    13441 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
    13501 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
    13561 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
    13621 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
    13681 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
    13741 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
    13801 ttgcgcacgt attcggtgaa cgcactatgg cgacactggg gcgtcttatg agcctgctgt
    13861 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
    13921 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
    13981 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
    14041 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
    14101 tcattacccg tttgaagtat gtatttcttg cagcaaaaat aatcaaaacc gcaagatatg
    14161 taatcatgaa gttgtcggaa aactatccgt acaagggagt gtatgaaaaa tgtctggtat
    14221 aataagaata tcatcaataa aattgagtgt tgctctgtgg ataacttgca gagtttatta
    14281 agtatcattg cagcaaagat gaaatcaatg atttatcaaa aatgattgaa aggtggttgt
    14341 aaataatgtt acaatgtgtg agaagcagtc taaattcttc gtgaaatagt gatttttgaa
    14401 gctaataaaa aacacacgtg gaatttagga aaaacttata tctgctgcta aatttaaccg
    14461 tttgtcaaca cggtgcaaat caaacacact gattgcgtct gacgggcccg gacacctttt
    14521 tgcttttaat tacggaactg atttcatgat gaaaaaatcg ttatgctgcg ctctgctgct
    14581 gacagcctct ttctccacat ttgctgccgc aaaaacagaa caacagattg ccgatatcgt
    14641 taatcgcacc atcaccccgt tgatgcagga gcaggctatt ccgggtatgg ccgttgccgt
    14701 tatctaccag ggaaaaccct attatttcac ctggggtaaa gccgatatcg ccaataacca
    14761 cccagtcacg cagcaaacgc tgtttgagct aggatcggtt agtaagacgt ttaacggcgt
    14821 gttgggcggc gatgctatcg cccgcggcga aattaagctc agcgatccgg tcacgaaata
    14881 ctggccagaa ctgacaggca aacagtggca gggtatccgc ctgctgcact tagccaccta
    14941 tacggcaggc ggcctaccgc tgcagatccc cgatgacgtt agggataaag ccgcattact
    15001 gcatttttat caaaactggc agccgcaatg gactccgggc gctaagcgac tttacgctaa
    15061 ctccagcatt ggtctgtttg gcgcgctggc ggtgaaaccc tcaggaatga gttacgaaga
    15121 ggcaatgacc agacgcgtcc tgcaaccatt aaaactggcg catacctgga ttacggttcc
    15181 gcagaacgaa caaaaagatt atgcctgggg ctatcgcgaa gggaagcccg tacacagttc
    15241 tccgggacaa cttgacgccg aagcctatgg cgtgaaatcc agcgttattg atatggcccg
    15301 ctgggttcag gccaacatgg atgccagcca cgttcaggag aaaacgctcc agcagggcat
    15361 tgcgcttgcg cagtctcgct actggcgtat tggcgatatg taccagggat taggctggga
    15421 gatgctgaac tggccgctga aagctgattc gatcatcaac ggcagcgaca gcaaagtggc
    15481 attggcagcg cttcccgccg ttgaggtaaa cccgcccgcc cccgcagtga aagcctcatg
    15541 ggtgcataaa acgggctcca ctggtggatt tggcagctac gtagccttcg ttccagaaaa
    15601 aaaccttggc atcgtgatgc tggcaaacaa aagctatcct aaccctgtcc gtgtcgaggc
    15661 ggcctggcgc attcttgaaa agctgcaata actgacgatg aggcccagga tattgggcct
    15721 cctttctttc tctttttttc ctgttgtcat ctacacttaa caaaaataca gcaaggaaaa
    15781 tcccatgcgc attttgcccg tcgttgctgc agttacggct gcattcctgg ttgtcgcgtg
    15841 tagctccccg acaccgccga aaggcgttac cgtggtaaat aactttgatg ccaaacgcta
    15901 tctgggaacc tggtatgaaa ttgcgcgctt cgaccatcgt ttcgagcgcg gattggataa
    15961 agtgaccgca acatacagct tgcgcgacga cggcggcatc aacgttatta acaagggcta
    16021 taaccctgac agggagatgt ggcagaaaac ggaagggaaa gcctatttca ccggcgaccc
    16081 aagcagagcc gcgcttaagg tttctttttt cggccccttc tatggcgggt ataacgtaat
    16141 tgcactcgac cgggaatatc gtcacgcgct ggtttgtggt ccggatcgcg actacctgtg
    16201 gatcctttca cggaccccta ctatttcaga tgaaatgaaa cagcaaatgt tagccatcgc
    16261 gacccgggaa gggtttgaag tgaataaact gatttgggtg aaacagcctg gcgcttagtg
    16321 agtgctcagc ttcagaccaa taatgccagc aacgatcagc ccaaggctca gcaaacgtgc
    16381 cgggctggca gactcaccca gcagcaaaat ccctgtaatg gccgccccaa cagcgccaat
    16441 accggtccag accgcataag cggttcctac aggcaacgtg cgcattgccc aagagagcat
    16501 ggcgatactg acgatcatcg ccgcaatagt gataatgctt ggcgtaagac gcgtaaaacc
    16561 gtgggtgtat ttcaggccaa tcgcccagac aacttcgagc aaacctgcaa ttaataaaac
    16621 gatccaggac atatcaggct ccagaacaat ggggccgtcc ccggtgaaag aagcgtttgc
    16681 aggtcgtcct gcaaagctaa tgtgtgaaat ggcatttttg cccggaagaa aatgaatttc
    16741 aaccttttta ttcaccgcct gctaaaagca agaattaagc ataattagcg gcgtagttcc
    16801 cgcatttggc actcatcgga gttctctatg atgaagggct ttctgaaacc agaaagtcga
    16861 gataacattc ctgcacagat cctcaaggaa ctcccttcat ggagtatcta tgtttcagcc
    16921 gaaaatctgc atactatact tcctacgtcc tatccgatac atggattttt cttttatcca
    16981 tttttggaca ataatggaaa tattatccgg cttttcatta ttgatgatct gaaacaaagt
    17041 caaggaacta cagggctaaa agaaaagaat gttgacgtcg tcaataatat catcagaata
    17101 aaggactctc gtgaaggact tcttgactcc agaaagatgg agtgtatcga tggtgaagtc
    17161 gtcgtaacag ttaacgaaaa gctcaaagat ttcagagaca gggagtttaa tcttcttaac
    17221 gcacagatat caatggtgct ttatatctgt tcacagatca atgatataaa agaaaaaaat
    17281 caatttaaac ggtcagaaaa gcacaaaaag catgttcaca ctcaccatga actaccagca
    17341 caaaacattc gggagtggga cgttggtatt cgaatggggc aagccatacg gcaatatcgc
    17401 caagcagaac ccacagggaa agagcggaca acaataggta gcaaacgtcc tcatatacgc
    17461 cggggacact ggcatacata ctggacagga agtaagaaac cagaactagc ccatgagcgt
    17521 aaacctagat taatatggtt acctccggta ccagttaatt tagaagacgt aaacaaacta
    17581 ccggtggtaa taacgcccat tgataaatga cctgagaagt atcttttccg gtaggatgta
    17641 cacatatcta aattatcagg ctactgtaat gttgttcatg cattacagta gagcccttgg
    17701 ttgattgata aaaaaatctc caggggcttt ctgctgtata ccgtccacaa aacgctttcg
    17761 gcatacagcc tcaagcgtcc gcggctattc taacgacttt cgtatctgca acaatccttt
    17821 tatcgcacgg ctaactcctc tggttagctc ttatcacctg aaaataaatt tttaaccttt
    17881 ccgtttgatt taaattccag aaatggagac tttgccatgt cttacaatcg acagcctgtc
    17941 gcagaagatc cgatgcagat atggggcgct gttggtgtcc ttcttatcct gctgctattc
    18001 gtcatatggt tattcttgcc ggaagtggtt tacgccagct gcctgattct tcatacctta
    18061 tggggactgg ttgactgggg accatttcat aactatgcag caccacgata caacctgctt
    18121 gccatgaccg gtaacaacgc cgccaacata agctatagcc agtgggttaa cgtcatggag
    18181 cagaccatag gtattttgtg gatgtacctc ttacctgtga cactctggtg tttgtgggaa
    18241 tggtatcaac atccgggaca aagccgtttc acccgcagac cagtagatat tacccgactg
    18301 ccacacatct ttgcttcgtt gtcccctgct atcgcccctg tactggaaga tggtgatccg
    18361 gaaaaactct ttcatggtgg caaacgacct gaacggcggg ttgctctcac cccggaagca
    18421 tttgtcgaac agcatactct catcaccaat atgcagctcg atgttgcagc tgcaagacga
    18481 tgtttcatgg cccagttggg taaaccttta acgtcctgga aagacatggc accacatgaa
    18541 aaagcgctct ttgccatatt tggccttcag tatttcctgg atgatcgtaa ggcagcatta
    18601 aagctcatgg atacactgaa cctctcatgc agaattaaaa gcaaacggga cagtgggaaa
    18661 ttctgtaccc ctgtttattc actggccaaa tctgcattca aaagagtgat taaaagcgac
    18721 ggagcacagc aatggctgaa acaacatcgc tacgtccgca gtgggctggt ctggctgtat
    18781 gcacatgact tgcggcttac gccacccaac tggatttggc tgaaaggcgt ggacagaacg
    18841 ctgttttatg cattacaccg cgccaatacc acgaaaggat ttattgaagg cgctggcgtg
    18901 gtggccgttg cacgtgcaga ggccgaagcc atgcgttttg gccttccctg ccctgaaccc
    18961 tgtgttgatg aagcggtgga aggcttacgg cgggatatgc tcagtctcgg tcttatctgg
    19021 gatgaaccac aaccagatcg cgaccggaaa cgccgcattc tgaccaactg gtccctcacc
    19081 gacgacatcc tgccccgcac acctgctaca gacaacgaat tctgattatc cggagaactg
    19141 aaaatgacaa tcgagtactt tgcaacacgc attgcaaaac atatttccgt gcaggcaatc
    19201 tggccgggtg gcaggacaga aattattgcc gtccttccac aggatgccct gtcagggctg
    19261 gtaacacgaa acaatcaact gattcatata gcctttgacg ccatgggcac tcgtaacaga
    19321 gaaaccgtcc tgattgacaa gcaggagcat aacgctgacc agatacttac agctattgat
    19381 tcctgtctgc gccttgaata cctgatggaa cgccgcttca gcagaacgcc tttgttccgt
    19441 gcaataatcg ccagcgttgt actgttcgta atggcaatga ttgccgtctc tctgttccgc
    19501 tatgttgatc gtgtattctg ggatgataca acgcctgaag cagtacagac ggcaggtgag
    19561 ccacgactgc tcccccctca cctgaatcac actgtgccat taaatgaagg cattcaactc
    19621 ccggttcccc cgaaaaaaga cgtccaggta ccggagaaaa cactcacaag caaaaatccg
    19681 gaagccgcag ctgcccgaca taatctggct gctgttctga agcggaacgc cgatcgggga
    19741 atgtttaccg tcaacctgtc atccggtcat gaacgaaccc tttacgcatt tctcgatcct
    19801 gcctgtccta actgcagact tctggagcca gccttaaaac gcctggccag cgacttcaat
    19861 gtcgttatct acccggtttc agttattggt ggagaggaat ccaccgaccg tgtggcacca
    19921 ctgctgtgtg aaaaagatgc tcagaaacgt gctgccggct ggcaccgact gtattccgca
    19981 gataacggaa tgatgacgcc atcagaagaa acaacgcctg cagatgaaac atgcctgaaa
    20041 gcggccagag ctgcgattga tgttaacaat gtcgccttcc gtaagttcgg atttgccggt
    20101 accccatggg tgctgtccga caccggctgg catctgccta ctggaattct gcaggaaact
    20161 ggcacgctta atttattcct gaaaaccacc gacagcgagt ctggccatga gtgaacatcg
    20221 cgttaacccc gagcttttac accgtacagc ctggggaaat cctgtctgga atgcactcca
    20281 gagtctcaat atctatggct tttgcctggt ggcaagtctt gtggccagtt ttatctggcc
    20341 acttgcatta ccggcatgtc tgttgttcac actgataacg atgctggtat ttagtctgca
    20401 gcgatggcgc tgccctcttc gtatgcccat gacgctggaa tgtgctgacc cgtcacagga
    20461 cagaatgatt aaaagaagcc ttttcagctt ctggccaact ctgtttcagt atgaggtcat
    20521 cctggagtca ccggccagcg gcatatttta tgttggttat caacgcgtca gggatatagg
    20581 tcgggagttg tggctgagta tggacgatct gacccgccat ataatgtttt tcgccaccac
    20641 cggtggcggt aaaacggaaa ccatctttgc ctgggcgatt aacccgcttt gctgggcaag
    20701 aggattcacc cttgtcgatg gcaaggcaca gaatgatacg gcaagaacga tctggtatct
    20761 ggccagacgc tttggccgtg aagacgatgt ggaagtcatc aacttcatga acggaggaaa
    20821 atcccgcagt gaaatcattc tgagtggtga aaaaacgcgt cctcagtcaa atacctggaa
    20881 tcccttctgt tacagcaccg aggcattcac agcggagaca atgcagtcaa tgctgcccca
    20941 gaacgtccag gggggcgagt ggcagtcccg ggccattgcc atgaataagg cactggtatt
    21001 tggtactaaa ttctggtgtg tgcgcgaagg caaaacaatg tcgctgcaga tgctccgtga
    21061 acatatgact ctcgaaggga tggccaaact ttactgtcgt ggcctcgatg atcagtggcc
    21121 ggaagaagct atcgcaccgt tacgtaacta ccttcaggac gttcccggtt tcgacctgtc
    21181 gctggtcagg acaccatcag cctggacgga agaacccaga aaacaacatg cttatctctc
    21241 cgggcagttt tctgaaactt tcagcacatt cacggaagca ttcggggata tttttgctga
    21301 agattccggt gatatcgata tccgtgacag cattcacagc gacaggattc tgatggtaat
    21361 gatccccgct ctggatacat cagcgcatac aacgtctgct cttgggcgaa tgtttatcac
    21421 ccagaaaagt atgattctcg cccgtgattt gggctaccgc ctcgaaggaa ctgattccga
    21481 tgcactggaa gtcaaaaaat ataaggggcg tttcccttat ctgtgtttcc ttgatgaagt
    21541 gggggcatac tatacggacc gtattgctgt ggaagccact caggtgcgtt cactggattt
    21601 tgctctgatt ttgatggcac aggaccagga acgtatagaa ggacagacaa cagccacgaa
    21661 tactgcaaca ctgatgcaga acacggggac aaaatttgcc ggcaggattg tcagcgaggg
    21721 tagtacggcc agaacgctga aaagcgccgc cggtgaagaa gccagggcga gaatgaacaa
    21781 cctgcagcgt caggacggca tatttggtga atcatggatt gacagcccac aaatcagcat
    21841 cctgatggaa agcaagataa atgttcagga gttgattgaa cttcatcctg gcgagttctt
    21901 ttctattttc cggggagaaa ccgtaccatc agcatcattc ttcatccctg acgatgagaa
    21961 aagctgcagc agcgatcccg ttgttataaa caggtatatc agcgtggatg cgccacgcct
    22021 ggaccgtctc cgccgtcttg ttccccgtac gactcaacgt cgaataccgt caccggaaaa
    22081 tgtcagtgcc atcattggcg tcctgactgc aaaaccctcc agaaaaagac gaaaaattcg
    22141 cacagagccg cacacgatcg tggatacatt ccagcaacgc attgcaggac gtcaggcggc
    22201 tatggcaatg ctggaagagt acgacacaga tatcaacgca cgtgaaagtg ccttgtggga
    22261 aacagcggtg aatacgctta aaacgaccac acgggaagag cgacgaattc gctatatcac
    22321 gctgaacaga ccagaactac ctgaaacaaa agaggaaaat cagatttcag tgagagcaga
    22381 aagagcaggt ataaacctgt taacgcttcc acaggacaat aaccacccga cagggcgtcc
    22441 tgtaaacggg tttcatcata aaaaaaacaa ccgtccggac tgggacggaa tgtactgaac
    22501 agtggccgcg ccggatttct ggcgcggcct tttttttaac ctggagaagg cggttcccag
    22561 cgtttatcct gctccggcgg aggcagtgat gcgtcatcgt tgctgttcgc cgcgatataa
    22621 tcgcggtagt agcgttcact tccttcacgg tgatgatcca cgtccggtcg cgtaatcctg
    22681 tagtcttcgt gaccggtacg gcctgtcaca ttatgccagg caaccatttc agcaaagacc
    22741 tggttaaatt gtttgtcgtt tccttctggt tcaaaaccca tgcgtggatc acgcccgaat
    22801 aataccggtg caatcaaatc cagtttatct gccagcgcat tgcggtcatg gtgattacgg
    22861 aaaacaaccc tgtcaccata atccgtacag acaaactctc ccgtccgacg gtcacggaaa
    22921 cgaacactcc cgttcttctg cagacgggcc tcgagatcac cggtattacc gtataccggc
    22981 gttccgcccg gttcacaaag cacgacacag cggtctgtcg tcgttgtgcg tgacctgtct
    23041 ttacggcgat cgcgataatc ccagcctctc aactgcgata cagcagccct gtctccctga
    23101 gcagcctgaa tttcgaccca ctgacggtaa gagggtggat accacttccc gtcagcaata
    23161 agtttctgtc gctcatccct gatgtcttct ttcagcctga tcaatgcctg cattcgctga
    23221 acttctgcaa tgtgataatg cagcttacgt aatgccggat catcgtactg cgcacgaatg
    23281 tgtgacttcc gcagacggca tgcctgatga atttcccgac aacgttcccc ataacgcaga
    23341 tctggtttac gccattgttc acgccatgcc agatagcggg cacgcaggtc agcacgggcc
    23401 tcagctctgg cttcacgtcg ctgccggcgc agcattgggt cacgtttact gctgaccccg
    23461 tacctgtcac tgacagccag ctccgggtta tagcgacttt caggttgcac cctgtcaaac
    23521 aaatctgctg gggcactcac aaaaggtccg gcctgtggct ctgcacgccc cagtgtcaga
    23581 tcaggatgaa tgctgctggc cttcaccggc gtttgctcat gattaaaggc atcaacgaca
    23641 acaagcccgt gatgttgttt ctgtagcatc agtccctgtt gtgcaaacag ctcatgacaa
    23701 cgctgccagg taaactcacc ttgtgtgatc atttcttcaa tctgagggct acaatggctg
    23761 atgctgtatc cccacagact ttctttgtca gaaaacagtt cgcgtttgat agccccctgc
    23821 ggccccgtgc ctgtacgcat acggtgccat tctttatcgg tcgcaggaca gctctccata
    23881 cgacgaccac ggaagcgttc tgttggcgta acctggcgga aaatatctgt tggtacaggc
    23941 tgccagccac catcgagttg attcacattg tcgagcgtca gcagcgacca tacgctgtca
    24001 gcacgaacgg gagtctggtt atgatcataa ccgtcacaga taaccaggtg accatgctga
    24061 acacgcatcc ataatcccgc ttcagccagt gtgcggtgaa tagcctggca gttttccagc
    24121 cgtccttccc gtgccatttc cggcagacgt tctccaaggt gacgacaggc atactgctgc
    24181 agactttctg tttcagcgat tttttccggg cgaacatcag cggcaatgaa gtccggatta
    24241 tagcgtcctg gtgcttccac ctggctgaaa atgtcttttg gtactggcgt gtagtcaccc
    24301 atttttttca tgagtttttc tgcacaccag gagggaccaa acgagtcaag ctgtacacct
    24361 tccctgttgc gatcccagcc atccatcacc agaaattttc cgtccatctg agacaggtac
    24421 agaccatctt ccgcaagacg acgatgcagg gataaccagt catttacagg ttcactgcgt
    24481 aaaccagcga ccgctgtctg cgcgacatac tcgcgaaaag tttgtttttt tccgcgtgtc
    24541 cacgcgttct ggcgatcacg ttcaacggca gttttgcgaa cgatacgatt tcccggcgca
    24601 tggacccagc aaccgttatc cggagcaaaa ccatgcttca gttcaagctc acggcaggca
    24661 cggctgagtt tttcctgact ccatgaaagg caattcagat aacctgtaac cggatgtacc
    24721 cggttgaccg caacgtgaac atgcaggtta tcagtatcgg tatgtacggc agaaacatac
    24781 tgatgttcac ccagcccgag ggatttcagg gtatggcgta cactgtcata aatctgttcc
    24841 ggacgcgggc tttcatgggc ctgccaggaa agaatgtagt gaaaaacagg atcagtatta
    24901 tctttcgcat agtgtgcctg ctgtgcaatg tactccatat ctgcagcagc agtttcaagc
    24961 gatgtacagt tgtgaaaaca ggtcacaccg taaaagttaa cccattcgca gccgtccttc
    25021 atgacatcaa ccagcgccac gaatgactca tttcgaaggc gtgtcgcata atcaacaagg
    25081 cgactgaaac ggctccggtg cggttgttca gcctgcgaag aagaagataa attcagttcc
    25141 tcatcggtca tgtcatcccg gaccgagacg taggacacca ggtcttcgaa cgaagacttg
    25201 ccgtcccttc tctttttcgg aatgactgca ttcaccggag aatcctcaac cttccattaa
    25261 ctgtttacgc aatgtatttg taagttcggt tatggcaaca agcacttctg cgtattcctt
    25321 gtcaccggtt ctttttccct gaacaaac
//
