LOCUS       NZ_CP029109             1565 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR436 plasmid unnamed1, complete sequence.
ACCESSION   NZ_CP029109
VERSION     NZ_CP029109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291529
            Assembly: GCF_003073875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 15.77x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003073875.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,875
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,581
            CDSs (with protein)               :: 4,581
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 63 of 175
            Pseudo Genes (incomplete)         :: 114 of 175
            Pseudo Genes (internal stop)      :: 32 of 175
            Pseudo Genes (multiple problems)  :: 32 of 175
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR436"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
     gene            1064..1543
                     /locus_tag="CSC23_RS00015"
                     /old_locus_tag="CSC23_0001"
     CDS             1064..1543
                     /locus_tag="CSC23_RS00015"
                     /old_locus_tag="CSC23_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000275450.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          604..684
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(229..489)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 aaccccgttt ttggacaatc tgattcaaat gttaacattt gacatcgggt tgtctatcct
       61 gcattgctct tttgccgcct caaaatccct tgcgtgtttt tgctccccga tctccagaac
      121 gatcgagccg ccacggttcc ggcagcgcct tgagcgcaac cggaaaccgt ctggggagaa
      181 ccccagaccc ccatcgctgt aacaccgacg gtctacgacc acctaccaac cgagtggctg
      241 cctgtaccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      301 cctggctagg ggtatcggcg cgcctgacct cctgaaacgc tctgtaagcg tttttcgcgc
      361 cttccatgcc ctgacgggca tttaaacccc gtttcgtgcg tctgagagct tctgagcgcg
      421 tttgaagggc tatctgacga cttatccccg aaagaacaac agcggaatca ccgacaacat
      481 gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa cgttagtgag cgagtgggcg
      541 aggaagcgga agagaaccgg aaaccacatt gagcacgtac gcactgatgc ggggtgtcgg
      601 ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg ggtgacggta ttacatttgc
      661 acatcctgtc ccgatttctg aggcgtttta gcggtgaacg gacggcaaaa ccatgatgga
      721 gctgttgaac gttgagagcg tgaaatctgg caaaaatcac agctggtaag gcttgcagag
      781 gagtaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca
      841 ccccaaaaaa gtgttccgct ttgcgcaaca ctttcagcag aaaagatatt taagggcgat
      901 tttgtaagtt attgattttt tagctacttg cattatgggt tagctcacgc atgtgtggtc
      961 taaggttagc tcacgcatgt gtgagctagg tcgacctatc atggtttacg tttgagctat
     1021 gatggcttat atttgagcca tcattagacc gtggagattt accatgtata tcgggactga
     1081 agagcagcga aaaaagcgtc taaaagaact ggaaaaatta aacaaagaat cggaatcaga
     1141 tccgaaaaat aacggcccat tcacccaggt ttatcagaag ggctgggaaa ggatccgaga
     1201 actgtcgaag gacaagcagg gagttgtagc aataccgctt tactcgtttt tagctgagca
     1261 catagatccc tcatgcggtg ctgttgttgc cgatcagcag tttttagcag ataaattggg
     1321 tgttagccga agcacaatta agcgctggct caactactta gaatcaaaaa acgcgttagt
     1381 cagaattcct gttgctggta aggtttgtgc gtatgcactc gatccacatg aagtctggaa
     1441 gggatacaac acatctaaaa actacgcggc gtttgtcact aaaacactgg tcaacaaaga
     1501 cggtgatatt cagcgccgaa tcatggccat gttttcaaat tgagccagcg gcaggcacag
     1561 gcaaa
//
