LOCUS       NZ_CP029106             1546 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR437 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP029106
VERSION     NZ_CP029106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291530
            Assembly: GCF_003073815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 12.87x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003073815.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:10:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 70 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 33 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR437"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
     gene            436..912
                     /locus_tag="CSC24_RS01115"
     CDS             436..912
                     /locus_tag="CSC24_RS01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(249..357)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(1145..1402)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.615"
                     /coverage="99.0"
                     /evalue="1.83e-109"
ORIGIN
        1 cgcgtgcgcg gtccgacatt cccacatcct gtcccgattt ctgaggcgtt ttaacggtga
       61 acggacggca aaaacatgat cgagctgttg aacgtggaga gcgcgaaatc gggcaaaatc
      121 atggctgacg aggcttgcag aggagtaagc cagtatacac tccgctatcg ctacgtgact
      181 gggtcagggc ttcgccccga caccccaaaa gggcgttgcg ttgcgcgcaa cacccttgcc
      241 tagaatagat cttttacgcc gatttgtaag ttgttgtttt tttagtgtgt tttttgttta
      301 cctgtcgcat ccagggtgac acaacgttgt cgcatcctgg atgcgacaac acgcaacaag
      361 gtgttgcaca gatgtgtcat gatggcttag atttgaacca acgttagtgc atgggatttt
      421 cagagggaaa aaatcatgtt tattgattca gaaaaacgac tgaaacaact ttcagatgag
      481 gcaaagaaaa acaccgagga tctcgaagaa gcaaagaaga attcaaggtt tacacaggta
      541 tccccaaaag gttgggaacg cgttcgagag ctgctgaagg atagccaagg catatcagca
      601 ctgaagctgt actcattttt agcggagcat atcgatccta cgtgtggcgc tgtcgttgcg
      661 gatcagcaat tcctagctga aaaacttgga gttagcagaa gcacaattat tcggtggctc
      721 aattacttag aatcaaaaaa tgcattagtt agaatccccg ttgctggtaa ggtttgtgcg
      781 tatgccctcg atccacatga agtctggaag ggatacaaca ctacgaaaaa ccatgcagcg
      841 tttgtcacta aaacactggt caacaaagac ggtgatattc agcgccgaat catggccatg
      901 ttttcaaatt gagccagcgg caggcggaca atcaggggct acgtgttaac gttttgacca
      961 tgattgtcta tcctgcattg ctcttttgcc gcctcaaaat cccttgcgtg tttttgctcc
     1021 ccgatcccaa gaacgaccgc gtcgccacgg ttccggcagc gccttgagcg caaccggaaa
     1081 ccgtctgggg agaaccccag acccccatcg ctgtaacacc gacggtctac gaccaccaac
     1141 cagccgaata gctgcctgta ccgtgcaaat aggctttgca gggagctgac gcggttccta
     1201 gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgag agcctctgta
     1261 agcgtttttc gcaccttcca tgccctggcg ggcatttaaa cccgtttcgt gcgtctgaga
     1321 gcttctgagc gtgtttgaag ggctatctga cgactcatcc ccgaaagaac aacaacggga
     1381 tcaccgacaa catgcgagag gtgatcccgc tcgccgcaag tcgatgaccg agcgtagcga
     1441 gcgaatcgac gaggaagcgg aagagaaccg gaagttacat tgagcactta cgcacggatg
     1501 cagggtgtcg gggtgaagcc ctgaccaggt ggggaatgtc tgagtg
//
