LOCUS       NZ_CP029105             2101 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR437 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP029105
VERSION     NZ_CP029105.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291530
            Assembly: GCF_003073815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4; SPAdes v. 3.6.0
            Genome Coverage                   :: 12.87x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003073815.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:10:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 70 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 33 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029105.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR437"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
     gene            complement(676..942)
                     /locus_tag="CSC24_RS01105"
                     /old_locus_tag="CSC24_0001"
     CDS             complement(676..942)
                     /locus_tag="CSC24_RS01105"
                     /old_locus_tag="CSC24_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(946..1911)
                     /locus_tag="CSC24_RS01110"
                     /old_locus_tag="CSC24_0002"
     CDS             complement(946..1911)
                     /locus_tag="CSC24_RS01110"
                     /old_locus_tag="CSC24_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1287..1518)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 caaaacccac tcaaagcctc gtcattactg ggctaaacca ctatttcata tccgccattt
       61 atcccttaag tataaataca aggatcacgc gcgagaattg cgcaaaaaat aaccacaatt
      121 ttaacccccc aaaacaagca cccccgaacg gcttacccca accttttcca cacacccgat
      181 caccaaaaca gggaataaaa acaaaaccct gtcttacagg tggatggcct cacttcgtga
      241 aaccatccac cccgcataag ttttttaatc ggtggtgtcg tcaatcacta aatcggaacc
      301 ccaaagggga cccccacgat ttagcatttg acgagattaa ctctcggaga tggcatcatg
      361 cttttaacgg actttaaaaa acggtgaacg gatgcggatt ttaatccgtg tctgtgaggc
      421 gttgagcaac gagcaaagcg aggcggtaga aagagagcca gcggcaggct caggacgata
      481 atccggggaa aatgtaccac tcgcagagtg ttaacatttg acatcggatt gtctatcctg
      541 cattggtgtc gagttggcaa ggctgaggat aatgctgagg ataatgctga ggataatgca
      601 ggatagacag agcactagag attgcatgca atctcgtacc tatcacctca gcctgccgct
      661 ggctcttttc cagaactaag aatgcgttct taaaaatcca aacttgatac cgtttaaatc
      721 tattttcaca aaaagagttt ttaccctctc gtttgttttc tgaatatata aaattaattc
      781 atcctcaaaa ccttcgtgcg gatgaaaacc aaatgtgcca aaacttaatc catcaataag
      841 caaaataagg tttttaaacc tgtcaggaat caccgcttca cttcttaaaa aaaataaacc
      901 aatagtctta tttccattca ggtattcaat attaatattc atacgttaat ccgatttatc
      961 cttctcaggg gcgcgtttgt aacgccgttt acctgaatcc cagacaaacg ccgtccgctt
     1021 cccgtcatca gtgccatccc ctacatcgtc ggcaatgaca agatcctcat tggtttctcg
     1081 atccaactgg aggacgtttt ttagcgcccc accggtcgag ataaaacggc gcttgtgaag
     1141 ctgccgcgtc agctcaagaa accactcagg atcgtttgcc atatcttccg gtttaacgga
     1201 gtatttcagc gtttcagcaa ccgcgctttg cagttgctcg gcaacgttgg ccacaacctc
     1261 acctgtctta gtttttactg cccgaatatc gatattcggc tcatagttca cccgcaagca
     1321 atcgcgccag agttctaccc aacgttcgtg cttaacgtag ttcttccctt taaaccaaga
     1381 aggttgcacc atcagcagac agtgaaaatg cggatgtgcg ctgccatcct tacctcgcgt
     1441 cacctccgta gccctgatcc acccctgaac aggtgatagc tcctttcgct tttccattcg
     1501 cttaaacgcc gcattcattg ctgtaaggac tgttccaagt tcacctatct cgcagttcct
     1561 gacagttaac gtcagaaaca accatcggga agacgggtaa tccacaacga ttttcggtag
     1621 agcctgataa aaacgggctt gccacatgag ggttcttctc cactggcaga cagggcaatg
     1681 acggacacgg caaaattgcg cactacgaag ccgtaggcgc gtttctccgg tttccatgat
     1741 ggtagaccaa ccaaaacgta acaaaccgct acaggaggcc atacggagtg catagcgctc
     1801 gaattcagca gcagtctggt agataccccc gacagaatcg gtaagcgtgc gctgggtatc
     1861 ccaaactgca tcacgggggc tgtagtccga aaggaattta tcttctgaca ttaagcctgc
     1921 tccgatctgg gcaggctgtg atttgatgtg gtatattcat cacgtacctg tgcattgcga
     1981 gccagtacat gtcaccagag tttgccgctg aggtgacaca ttcgaaatgt tggggagtgt
     2041 tgacgcactc cccaacgccg attaaaaacg cctcaattct gctccatttt ctttgacctg
     2101 t
//
