LOCUS       NZ_CP029098            11611 bp    DNA     linear   CON 04-JAN-2024
DEFINITION  Klebsiella pneumoniae strain AR438 plasmid unnamed1.
ACCESSION   NZ_CP029098
VERSION     NZ_CP029098.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291531
            Assembly: GCF_003073675.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0; HGAP v. 3.0
            Genome Coverage                   :: 15.39x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 17:57:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,856
            CDSs (total)                      :: 5,732
            Genes (coding)                    :: 5,602
            CDSs (with protein)               :: 5,602
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 58 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 15 of 130
            Pseudo Genes (multiple problems)  :: 18 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AR438"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
     gene            <1..1178
                     /locus_tag="CSC25_RS00005"
                     /old_locus_tag="CSC25_5937"
                     /pseudo=""
     CDS             <1..1178
                     /locus_tag="CSC25_RS00005"
                     /old_locus_tag="CSC25_5937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /name="conjugal transfer protein"
     gene            1188..1958
                     /locus_tag="CSC25_RS00010"
                     /old_locus_tag="CSC25_5938"
     CDS             1188..1958
                     /locus_tag="CSC25_RS00010"
                     /old_locus_tag="CSC25_5938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000716326.1"
                     /translation="MKKHIVAALIASVTVISGAQAGVPVAIDANPEWAVEAQRWTERLK
                     QWQDTVNHYQKQINAYKQELLTKTGIRDVQGLVQSAQSVSKELENIYDQGNSFIDDYIK
                     NPEATLSEQARSLLADYKVTNTCKGLGYTGDLVRGCEASFLSQLAGIEYGNKLESKLRE
                     DNQEMADLIDQVKNAQDTKATQDATNAVSLASLKFNKLKFQYEMYRDKQRDLAEYKEKM
                     LQAAFQEKQLGAVNKETPVVDYKAAFEQQSYEMN"
                     /name="type IV secretion system protein"
     gene            1968..2225
                     /locus_tag="CSC25_RS00015"
     CDS             1968..2225
                     /locus_tag="CSC25_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000759841.1"
                     /translation="MKKYGLVAALGLTFFLTGCEEVKSVDWWQSHVDDATKKVAECKKS
                     GSDSENCKNAKEGLFRYKQLHPKHVDYKDAFKNVLGKDGS"
                     /name="EexN family lipoprotein"
     gene            2237..3292
                     /locus_tag="CSC25_RS00020"
                     /old_locus_tag="CSC25_5939"
     CDS             2237..3292
                     /locus_tag="CSC25_RS00020"
                     /old_locus_tag="CSC25_5939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000235886.1"
                     /translation="MATSRYFQAAHDVIVKTLDQSLTGQLDNFSSIASQLGKYGISIYI
                     LWYAYTTIVGKQKTAVQDFLWNLCRFWLILVFVKNMGGWLDSATQAIDGLKETFAGGDP
                     WKWVDELWEKVQQVAAYLMSKDTSKYVKTDGAIASLLTYAGGIIALLLTSIVYLAAEVT
                     IKILTITAPLFIICLSFGFLRQMFNSWLQLIFSSCFIFLFCGLAIKAGMTFLNGILTIS
                     IANADELNLISTGAQAGVAGAFMAWIIWQAKTYASQLAGVGVEGAMQGAAAMGIGAGVF
                     GASRMGRNLLGMGKNAGIGAWKGVRRQDGGFNQSPGVSGKLGNLTGQGVNIGAKRLRQA
                     AIDAAKKKYGG"
                     /name="type IV secretion system protein"
     gene            3487..4215
                     /locus_tag="CSC25_RS00025"
                     /old_locus_tag="CSC25_5940"
     CDS             3487..4215
                     /locus_tag="CSC25_RS00025"
                     /old_locus_tag="CSC25_5940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_004199085.1"
                     /translation="MGFKLSGLKLPGFNKKKEVTDSSTTFEEKNVLMQEKMNRIYKFGG
                     IAGIAVGVLSLLALNGALPLKSTVVEAYLINGVTGVAERLTSVKKENLSENEALAKYFI
                     TQYVKRREGYNYFSLQHDYDYVLLYSAENVATDYNALINGNQSPKVIYNKAEKTASVQD
                     NPSVIISPSSRRDDKDMGAYIRFKLTIRNVATGQDDYEYWNVRLTYRIEPQVEMASGDR
                     NNNPLKFVVTSYVRDKEVKG"
                     /name="type IV secretion system protein"
     gene            4221..5150
                     /locus_tag="CSC25_RS00030"
                     /old_locus_tag="CSC25_5941"
     CDS             4221..5150
                     /locus_tag="CSC25_RS00030"
                     /old_locus_tag="CSC25_5941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="WP_000783386.1"
                     /translation="MKMNKGALIVALLLVSHVCHAAVTPAASRFDPRNQIVSYNPQNTT
                     VINSAVGYTTTLVFDEDETVISARTGFPQGWEVNKEDNLVYLEVKPVKQTVQKNSTDDN
                     GNQSSEVVSVALDPENDLERWRTNLFVRTTKRNYSMELNARTFRQPDKIAFVVNYQYPQ
                     ERRKEQAEIEKKRIEALNKRQEEQAINRSLENAKSPKNWDYWKRVADGSQSISPDYAYD
                     DGRYTWFGFSPLKKIPSVFVMSGEQETLTNPVVKNSGSFTVVGVPVDQRFVLRMGNQVV
                     GVENRGFGKVRLPAGDTVSPNVEKEVIQ"
                     /name="TrbG/VirB9 family P-type conjugative transfer
                     protein"
     gene            5147..6361
                     /gene="virB10"
                     /locus_tag="CSC25_RS00035"
                     /old_locus_tag="CSC25_5942"
     CDS             5147..6361
                     /gene="virB10"
                     /locus_tag="CSC25_RS00035"
                     /old_locus_tag="CSC25_5942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_001295060.1"
                     /translation="MSEQENKIPTAVEIEQALRERHQKEIEQNDKTDKGDEDDGKSVKR
                     LGIEKLKKSRKGLVIIVAAFLLLAAGVSVYYIPSIIRAMSSGDDKPASNAVSTGSVKRA
                     TGLSDDVDPFNTREEPAKTEERKESSGKAETPPEKVQQNFSRALDVAYGGSSSASSNSG
                     GSSSTASNTRNEGDGSDKQAEVQPVNAGQPAQLSKITRVPYDPNLFIPENTAIKCSLDR
                     RFISDLAGKLVCTINEDVYSANRNVKLIEKGTAAYLMYKTGTFNHGQGAVFIAATKLRT
                     RKEPFIDIPLIDTQAAGALGEAGASGWIDTHFADRFMGAMMVGMIPDVAQAASGAAKSN
                     KDNQTDYTANSRQAFADIAREAFANSVNIPPTLYKNQGEIITLIVGQDLDFSSIYKLKM
                     VGTRR"
                     /name="virB10"
     gene            6363..6560
                     /locus_tag="CSC25_RS00040"
     CDS             6363..6560
                     /locus_tag="CSC25_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_000629109.1"
                     /translation="MITLDIKEFSMLLGIRESEIYHHIRKGIPINGVPFPKPLKQIKTH
                     RFNYEEVMRFIEDLKGKGEL"
                     /name="DNA-binding protein"
     gene            6557..7591
                     /gene="virB11"
                     /locus_tag="CSC25_RS00045"
                     /old_locus_tag="CSC25_5943"
     CDS             6557..7591
                     /gene="virB11"
                     /locus_tag="CSC25_RS00045"
                     /old_locus_tag="CSC25_5943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549925.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000650512.1"
                     /translation="MKDAENRHIVYEIIDEYFHHWLNEVDGLTEIAVNRPDELFVKVSG
                     KWQQHELKMDFKDCMSFAGAISDYHDGGSVTPEYPLRSVTLPGGERVQIVIPPATERET
                     VSITIRKPSSVFIDHDTFVKQGFYSRLNQGGKFRDKEDELSSMFKDNCFEQFVPECLVK
                     GKTMVFCAGTGAGKTTFANACLQYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPSEGES
                     STVSSASLLRSGFRMNPDRILMTEVRGAEAWDFLKGSSSGHAGNLTTVHESTPEDAVLG
                     LVQRCYMNPECQNLPYNIILRRVLSNVDVIMSIKYIDEEDNRFASGIYYRDIHFQEYFE
                     KLKE"
                     /name="virB11"
     gene            7594..9429
                     /locus_tag="CSC25_RS00050"
                     /old_locus_tag="CSC25_5944"
     CDS             7594..9429
                     /locus_tag="CSC25_RS00050"
                     /old_locus_tag="CSC25_5944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_000053822.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNF
                     DSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFAS
                     DNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLV
                     RKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLFYIDLKEESGAKD
                     LLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKPVFSIG
                     SVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQRSSIDGS
                     FRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNLFFNFVVE
                     VTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNE
                     IYGVEGAKTLMSAHPCRIIYAVSEEDDAKKISEKLGYITTKSTGSSKTSGRSTSKSSSE
                     SEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPYFMDRLMMVSPKLTELT
                     ASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            9426..9818
                     /locus_tag="CSC25_RS00055"
     CDS             9426..9818
                     /locus_tag="CSC25_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_000738524.1"
                     /translation="MKKMLMVSVLFLSACSSPPEPPQVDWEQNPETVNTQLMDWQPTYS
                     VIKSDKVNSSWVKVIHNFRPENRLYDDAVFYSVAHSDSVIVETSNGTDFFTAKNWLRAN
                     GANGVIQYRYKMNCFSCRTTSVYLSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            9906..10220
                     /locus_tag="CSC25_RS00060"
     CDS             9906..10220
                     /locus_tag="CSC25_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000754496.1"
                     /translation="MKKTVTTAVLLCAFAAGTAHAEEKADPNDPCAMVLCLAGKLDGSS
                     PAECDPMYKSFMSIRKKNKHGFLPDHTADARKKKLNECPAADAGTVSKIISSFGRLKNF
                     "
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            10306..10632
                     /locus_tag="CSC25_RS00065"
     CDS             10306..10632
                     /locus_tag="CSC25_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004208538.1"
                     /translation="MKIINVPDARSVRIAGIEIIDSVKSVASVFTEILLFMVWFPAIVY
                     GLYRFVIVLWDASLKPQSLIEFINFMVELYSYVLPSLAVIAVALVVVLSIFKRLSAIWE
                     GRKQ"
                     /name="hypothetical protein"
     gene            10629..11123
                     /locus_tag="CSC25_RS00070"
     CDS             10629..11123
                     /locus_tag="CSC25_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_001215543.1"
                     /translation="MRHFFNVLSFAVALLPATLCAAQIQGKVIRVLDGDTIEVKTLPAK
                     IVVYEVPIRVRLINIDAPEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRVLGRVV
                     TANGTEANRQQVLKGAAWVYDRYNTDNSLPALQREAQTQKRGLWADSNPVPPWEWRHKQ
                     N"
                     /name="thermonuclease family protein"
     gene            11206..>11611
                     /locus_tag="CSC25_RS00075"
                     /pseudo=""
     CDS             11206..>11611
                     /locus_tag="CSC25_RS00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     Typing          2238..3289
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3488..4212
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.18e-145"
     Typing          4222..5147
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.706"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7595..9390
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="95.159"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 agcatcaaaa ttacttcggt aaaaacccca cgctggggca taccgaaatt cttggtacgt
       61 caaacgtggg taaaacggta gtgatgatga ctaaggcttt cgccgcccag cagttcggaa
      121 cgccggagtc ttttccacct gagaggaaat taaagaagtt aactacagta tttttcgaca
      181 aagatcgggc agcggaaccc ggtatacgtt caatgggagg tgcttacttt cgggtgaaag
      241 aaggagagcc gactggctgg aaccctgcgg ctttgccacc aaccaaacga aatatttcct
      301 ttatgaaaga cctggtgagg ctactttgta cgcttaacag tgagccgctt gatgattacc
      361 agaaccgcct gatttctgat gccgttgagc gcctgatgca gaggtcaaac cgctcttatc
      421 cgatcagtaa gttgcgacca ctcattctgg agcctgatga tactgaaacc cggcggcatg
      481 ggcttaaagc acgcctgaat gcctgggtgc agggagggga atttggctgg gtgtttgata
      541 acccggacga tacgtttgat gtggacaacc tggacgtttt cggtatagac ggaacggagt
      601 ttctggataa caaagtactt tccagcgccg cttcattcta tctcatctac cgtgtcacca
      661 tgctggctga tggccgccga ctccttatct acatggatga gttctggcaa tggattaaca
      721 acgaagcgtt cagagatttt gtttataaca aactcaaaac cgcgcgtaag ctggatatgg
      781 tgcttgtcgt agcgacacaa tcgccggatg aactaattaa atcacctatc gcggctgccg
      841 ttcgtgagca gtgcgcaaca catatttatc tggcgaaccc caaagcgaaa cgtagtgagt
      901 acgtagacga gttggaagtg agagaacttt attttgacaa aattaaagcc attgacccgt
      961 tatctcgtca gttcctggtc gtcaaaaacc cacagaggaa gggggaaagt gatgattttg
     1021 cggctttcgc aagactggat ttgggaaaag cggcgtatta cctaccggtt ctcagcgcat
     1081 cgaaacccca gcttgaactg ttcgatgaaa tctggaaaga gggtatgaag ccggaagagt
     1141 ggcttgatac ctatctggaa cgtgcaaacc tgatttaagg aatccctatg aaaaagcata
     1201 tcgttgcggc acttatcgcc tctgttacgg ttatttctgg tgctcaggct ggtgttcccg
     1261 ttgccattga tgcaaaccct gaatgggctg tcgaagcgca gcgctggaca gaacgcctca
     1321 aacagtggca ggacaccgtg aaccactacc agaaacagat taatgcctat aagcaggagc
     1381 tgctgaccaa aacgggcatt cgtgatgttc agggactggt gcagtctgcg caatctgtca
     1441 gtaaggagct ggagaatatt tacgaccagg ggaactcctt cattgatgat tacattaaga
     1501 acccggaagc caccctgtct gagcaagcca gatcactact ggccgattat aaggtgacaa
     1561 acacctgtaa ggggctggga tatacgggcg acctggtgcg gggctgtgaa gcctcgttcc
     1621 tgtcccaact ggcggggatc gagtacggca ataagctgga aagtaaactg cgtgaagata
     1681 atcaggaaat ggccgacctt atcgaccagg tgaagaacgc ccaggacacg aaagccacgc
     1741 aggacgcgac aaacgctgta tctctggcaa gcctgaaatt taacaagctc aaatttcagt
     1801 atgaaatgta tcgcgataag cagcgcgatc ttgcagaata caaggagaaa atgttacagg
     1861 ctgctttcca ggagaaacaa ttaggggctg ttaataaaga aactccagtt gttgattata
     1921 aggcagcatt tgagcagcag agttatgaaa tgaactgagg gggcattttg aaaaaatatg
     1981 gattagttgc ggcgttgggg cttacattct ttcttaccgg gtgtgaagag gttaaatctg
     2041 tagactggtg gcagtctcac gtagacgatg caacgaaaaa agtagctgaa tgcaaaaagt
     2101 ctgggagtga tagcgaaaat tgtaaaaacg ctaaggaagg tttgtttagg tataagcaat
     2161 tacatcctaa gcatgttgac tataaagatg cattcaaaaa tgtattgggt aaagatggaa
     2221 gttaaaagga gccactatgg caacttctag gtatttccag gccgctcatg acgttattgt
     2281 taaaactctt gaccagtctt tgactggtca gttagataac ttttcttcaa tagcgtctca
     2341 gttgggtaag tatggtattt ccatttatat attgtggtat gcttatacca caatagttgg
     2401 gaagcaaaaa accgcagtgc aggatttttt atggaatcta tgtcgattct ggttaattct
     2461 tgtttttgtc aaaaatatgg gggggtggtt agatagtgca actcaggcca ttgatgggct
     2521 gaaggaaaca tttgcaggag gtgatccttg gaaatgggtt gacgagcttt gggagaaagt
     2581 tcagcaggta gctgcatatc ttatgtccaa agatacttcg aagtatgtga aaacagatgg
     2641 ggcaatcgca tctttattaa catatgctgg agggataatt gcattattgc taacttcaat
     2701 agtttatctc gccgccgaag ttactattaa gatacttact attaccgcgc cgttgtttat
     2761 tatttgtctt tcatttggtt ttttaaggca gatgtttaat agctggcttc agttaatatt
     2821 tagctcatgc ttcatattct tgttttgtgg actggcaatt aaagcgggaa tgacgttcct
     2881 taatggcatt ctgacaatct cgatagctaa cgcagatgaa ttaaatctga tatcaacagg
     2941 tgctcaggcc ggggttgcag gtgcatttat ggcctggatt atctggcaag ccaaaaccta
     3001 tgcttcccag ctcgcagggg taggtgttga aggcgcgatg caaggggcag ccgcaatggg
     3061 gattggtgct ggcgtgttcg gcgcttcccg tatggggcgt aatctgcttg gtatgggtaa
     3121 aaatgcaggg attggcgcat ggaaaggcgt tcgccgtcag gatggagggt tcaaccagtc
     3181 tcctggtgta tccggtaagt tggggaatct gaccgggcag ggcgttaata ttggcgctaa
     3241 gaggcttcgc caggctgcga tagatgcggc gaaaaagaaa tatggaggtt aaccgattta
     3301 ttatttaaat ctgtcaactc tgagtattcc gcagtaaata ttcactcctc tatttaggtt
     3361 ctcacattat gaaactactt atcacggcta tcgtgacgct ttgccttgtc ggatgccagg
     3421 cgtcacataa acaacctccg gtatccggaa aaagcgaacc tgttaattca gcagaggtaa
     3481 tgcgcaatgg ggtttaaact ctcaggatta aaacttcccg gttttaacaa gaaaaaagaa
     3541 gtaacagact cttcaactac ttttgaagag aaaaatgtat tgatgcagga gaaaatgaac
     3601 cgcatttata aattcggtgg tattgcagga attgccgtag gtgtgttgtc tctgctggca
     3661 ctgaatggag cgctaccgtt aaagagtacg gttgtcgaag cttatcttat taatggtgtc
     3721 actggcgttg ctgaaagact gacttctgtt aaaaaagaaa acctctctga aaatgaagcg
     3781 ctggcgaaat acttcattac tcagtatgtg aagcgtcgtg aagggtataa ctatttcagt
     3841 ctccagcatg attatgatta tgttctgtta tacagcgctg agaatgtggc gacggattac
     3901 aacgctttaa tcaacggcaa ccagtcacca aaggttattt ataataaagc ggaaaaaacc
     3961 gcatctgttc aggataatcc gtccgtgatt atcagtccgt catcccggcg cgatgataag
     4021 gatatgggag catatatccg gtttaaatta accattcgaa atgtcgcgac aggccaggat
     4081 gattatgaat actggaacgt ccggttgacc taccgtattg aaccacaggt cgaaatggca
     4141 tcgggtgacc gaaacaataa cccgcttaaa ttcgttgtaa cgagctatgt acgcgataaa
     4201 gaagtgaaag ggtgaaaact atgaaaatga ataaaggagc gctaattgtg gcgcttctgt
     4261 tggtgtcgca cgtctgccat gcagccgtta cgccagcagc cagccgtttt gatccccgta
     4321 atcagattgt cagttataac ccgcaaaaca cgacagtcat aaacagtgcg gttggctata
     4381 caaccacact ggtatttgac gaggatgaaa cggtcatcag tgccagaacc ggctttcccc
     4441 agggctggga agtgaacaaa gaagataacc tggtgtatct cgaagtgaag cccgtcaaac
     4501 agaccgtaca gaagaacagt accgatgaca atggcaacca gtcttcagag gtggtcagtg
     4561 tagcccttga tcctgaaaac gacctggaac gctggcgaac aaacctgttt gttcgcacaa
     4621 caaaacgtaa ttacagcatg gagctgaacg cccgaacgtt ccggcagccg gataagatcg
     4681 cgtttgttgt taattaccag tacccgcagg aacgccggaa ggagcaagcc gaaatcgaga
     4741 agaaacggat tgaggctctt aacaagcggc aggaggagca ggcaatcaac cgttctcttg
     4801 aaaatgcgaa gtcgcccaag aactgggatt actggaagcg ggtagctgac ggcagccagt
     4861 caatcagccc ggattatgcg tatgacgatg gccgctatac ctggtttggc ttcagccccc
     4921 tgaagaaaat ccccagcgtc tttgttatga gcggcgaaca ggaaacgctg actaacccgg
     4981 tagtcaaaaa cagcggtagt ttcaccgttg ttggtgttcc tgtagaccag cgctttgttt
     5041 tgcgtatggg aaatcaggtt gttggggttg aaaaccgggg ctttggcaaa gtccgtcttc
     5101 cggcaggaga taccgtttcc ccgaacgttg agaaagaggt gatccagtga gcgaacagga
     5161 aaataaaatc ccgaccgctg tagaaattga acaggcacta cgggaacggc atcagaaaga
     5221 aattgagcaa aacgataaaa cggataaggg agatgaagat gacggtaaat cagtaaaacg
     5281 cctgggtatc gagaagctta aaaaatcgcg taaggggctg gtgattattg tggccgcttt
     5341 ccttctgctg gccgccgggg tgtctgtcta ctatatcccg tcgattattc gtgcaatgtc
     5401 cagcggtgat gataagcctg ccagtaatgc ggtatctact ggttcagtaa aacgcgcgac
     5461 aggactcagc gatgatgttg atccattcaa caccagggaa gaaccggcta agacggaaga
     5521 acggaaggaa agcagcggca aagcggaaac gccaccggaa aaagtacaac aaaacttcag
     5581 tcgtgcgctg gatgtggcct atggggggag cagctctgct tcctcaaatt ccggtggtag
     5641 ctcatcaacg gccagcaata ccaggaacga gggggacggc agtgacaaac aggcagaggt
     5701 tcagcctgtt aatgcagggc aacccgcgca gctttctaaa attacgcgcg ttccttatga
     5761 ccctaacctt ttcattcctg aaaataccgc gattaagtgc tcgctggaca gacgatttat
     5821 ttctgatctg gctggcaagc tggtctgtac gattaatgag gacgtataca gtgcaaacag
     5881 gaatgtaaag ctaattgaaa agggaacagc cgcatatctg atgtataaaa cgggcacgtt
     5941 caatcatggt cagggagccg tgtttatcgc tgctacgaag cttagaacac gtaaggaacc
     6001 tttcattgat attccgctga ttgatacaca ggcggcggga gcgctgggtg aggccggggc
     6061 ttcaggctgg attgacacac attttgcaga tcgctttatg ggcgcgatga tggttggcat
     6121 gatccccgat gtggcacagg ccgcaagtgg agcggctaaa agtaataaag acaatcagac
     6181 cgactataca gctaacagtc gccaggcttt tgctgatata gcacgcgaag cgtttgcaaa
     6241 cagcgtaaat atccctccaa cgctttataa aaaccagggg gaaatcatta ctctgatcgt
     6301 tggtcaggat ttggatttct ccagcatcta caaattaaaa atggtgggta cccggaggta
     6361 atttgataac gctggatatc aaagagtttt caatgctgtt aggaataagg gaaagcgaaa
     6421 tataccatca tattcgtaag ggcataccaa taaatggcgt tcctttcccc aaaccactga
     6481 aacaaatcaa aacgcatcga tttaattacg aagaagtcat gcgttttatt gaagatctta
     6541 aaggaaaagg tgaattatga aggatgcaga gaacagacat attgtttatg aaataataga
     6601 tgaatacttc caccactggc ttaatgaagt cgatggttta actgaaatcg cggtaaatcg
     6661 gcctgatgaa ctgtttgtta aagtgagtgg gaaatggcag cagcatgaat taaaaatgga
     6721 ttttaaagat tgtatgtcat ttgcaggggc aatttctgat tatcatgatg gtgggtcggt
     6781 tacgccagaa tatcccttac gttctgttac gctccccgga ggggagcgtg tacaaatagt
     6841 tattcccccg gcaacggaac gggaaacggt gtctattaca atccgtaagc cgtccagtgt
     6901 gtttatcgac catgatacct ttgttaaaca agggttttac tcaaggctaa atcaaggggg
     6961 gaaattcagg gataaagaag atgaattatc ttcaatgttt aaagataatt gttttgaaca
     7021 atttgtccct gaatgcctgg ttaagggtaa aacgatggta ttttgtgccg gaacgggggc
     7081 gggtaaaacc acctttgcaa atgcctgtct tcagtacata ccccatcatt tacgatgtat
     7141 ttcaattgaa gatacagatg aagcaaagtt caggttccac aaaaatcatg tgaaattgta
     7201 ttacccgtca gaaggggaaa gtagcactgt cagttccgct tctcttttac gttctggttt
     7261 tcgtatgaac ccggacagaa tcctcatgac tgaggttcgc ggcgcggaag cctgggattt
     7321 tctcaaaggc tcaagttccg gccatgcagg gaaccttaca accgtccatg agagcacccc
     7381 tgaagatgct gtgcttggac tggttcaacg gtgctatatg aaccctgaat gtcagaattt
     7441 gccttataac attatattgc ggagggtgtt aagcaacgtc gatgtgatta tgagtattaa
     7501 gtacatcgat gaggaagata accgatttgc atcgggtatt tactatcgtg atattcattt
     7561 tcaggaatac tttgaaaagc tgaaggagtg attatgtctc tgaaactacc cgataaaggc
     7621 cagtgggctt ttatcggtct ggttatgtgt cttgtgacgt attatactgg ctcagttgct
     7681 gtttacttcc tgaacggaaa aacgccgctt tatatatgga agaattttga ttccatgctg
     7741 ctgtggcgga taataacaga gagtaatata cggacagata tcaggttaac cgcaatcccc
     7801 tctcttttat caggtatggt ttcgtctctc attgtgcctg tttttattat ctggcaactg
     7861 aataaaacgg atgttgctct ttatggtgac gcgaagtttg ccagtgataa tgatttaaga
     7921 aaatcgaaac ttctgaaatg ggagaaagaa aacgatactg atattcttgt aggtgcatat
     7981 aaaggtaaat acctgtggta taccgcacca gattttgtat cacttggcgc aggaacccgc
     8041 gcaggtaaag gggctgcaat aggtattcca aacctgctgg tcaggaaaca ttccctgatt
     8101 gcattagatc caaaacagga actgtggaaa atcaccagta aggtacgtga aaaactgctg
     8161 ggtaataagg tctatcttct tgaccctttc aacagtaaaa cgcatcagtt taacccgctt
     8221 ttctatattg atttaaaaga agagagcggg gctaaagacc tgctgaagct gattgagatc
     8281 ctgttcccgt cttacggtct gaccggagcg gaagcacact ttaataacct cgcggggcag
     8341 tactggacgg ggctggcaaa attgcttcat ttctttatta actacgaccc ctcctggctt
     8401 ggtgagttcg ggcttaaacc tgttttctca attggttctg tagtcgattt gtacagcaac
     8461 atcgaccggg agctgatact cagtaagcga gaagacctgg agggaacaaa aggacttgat
     8521 gaaaacgcgc tgtatcatct gcgcgatgcc ctgaccaaaa tcagggagta tcacgaaacc
     8581 gaagacgaac aacgttcgag tattgatggt tcgtttcgta agaaaatgag tctgttttat
     8641 cttccgactg ttcgcaaatg tacagacggc aatgatttcg accttcgcca gttgcgccgg
     8701 gaagatatca ctgtttatgt tggcgttaat gctgaagata tgtccctggc ctatgacttc
     8761 ctcaacctgt tctttaactt cgttgtggaa gtcacgttac gagaaaaccc tgactttgac
     8821 ccgacactga aacatgactg tctgatgttc ctggatgagt ttccgtctat tggttacatg
     8881 ccaatcatta aaaaaggttc gggttatatt gccgggttta aactcaaact tctgacgatt
     8941 taccagaata tcagtcagct taatgaaatc tatggtgttg aaggtgccaa aacgctgatg
     9001 agtgcgcatc cctgccggat tatctacgct gtcagtgaag aagatgacgc taaaaagatt
     9061 tcggaaaagc tgggctatat caccaccaaa tcgacaggtt caagcaagac aagcggacgc
     9121 tcaacgtcga aaagcagttc agaaagtgag gcgcagcgcg cccttgtgct cccgcaggag
     9181 ctgggaacgc tggattttaa agaagaattt atcattctta aaggtgaaaa cccggttaaa
     9241 gctgagaaag ccctctactt cctcgacccg tattttatgg acaggctgat gatggtaagc
     9301 cccaaactta ccgagttaac cgcctctctg aataagacta aaaaagtatt gggtgttaaa
     9361 gggcttaaat atccttctaa agaaaaaatg ctctccgtcg gtgagctgga gtcagaggtt
     9421 ttactatgaa aaaaatgtta atggtcagtg tcctttttct ctctgcctgt tcctctccgc
     9481 ctgaaccacc acaggttgac tgggaacaga atcctgaaac ggtgaataca caattaatgg
     9541 actggcaacc gacatactca gttattaaat cagacaaggt taattcatcg tgggttaagg
     9601 ttatccataa tttcaggcct gaaaaccgcc tttacgatga tgctgttttt tattctgttg
     9661 cacattctga ttcagtgatt gttgaaacca gtaacggaac agactttttc acagctaaaa
     9721 actggttgag ggcgaacggt gcaaacggtg tcatacagta tcgttataaa atgaactgtt
     9781 tttcttgccg gactaccagt gtttatttat cacgataacg ccggattaat aaaattcttg
     9841 taattttatt gagccggggt tgaacaaccg cagggcgtta gttgcccgtt atccatgagg
     9901 tgtatatgaa aaagaccgta accacggcag tgctgctttg tgcgttcgct gccggaactg
     9961 ctcatgctga ggaaaaggcc gacccgaacg atccatgtgc aatggtgtta tgccttgccg
    10021 gaaaacttga cggaagtagt ccggcagaat gtgacccgat gtataaaagt tttatgtcaa
    10081 tcaggaaaaa gaataaacat ggttttttac ccgatcacac ggcagatgcc agaaagaaaa
    10141 aactaaatga atgtcctgcg gctgatgcag gtacagtaag taaaattatt tcaagctttg
    10201 gccgattgaa aaacttttaa ttctgtttga tttaattctg atgtgaatac gcaagtttca
    10261 ggtttgagac tggaacttgc gaacattttt tacaggggtg ggaggatgaa aattattaac
    10321 gtacctgatg caagaagtgt gagaattgcc ggaattgaaa ttatcgatag tgtcaaatcg
    10381 gtcgcttctg tttttactga aattttgttg tttatggtct ggtttccggc aattgtctat
    10441 gggctttatc gttttgttat cgttttgtgg gatgcttcct taaagccgca atcgctcatt
    10501 gagtttatta atttcatggt tgagttatac agctatgtac ttccttcgtt ggctgttatc
    10561 gcagtggctt tagtcgtcgt tttatccatt tttaaaagac tctcagcaat atgggaggga
    10621 agaaagcaat gagacatttt tttaatgttc tttcctttgc tgttgccctg ttgccagcta
    10681 cattgtgtgc tgctcagatt caggggaaag ttattcgcgt tctcgatgga gataccatcg
    10741 aagttaaaac actacccgcg aaaattgttg tgtatgaagt tccgattcga gtccgattga
    10801 taaatatcga tgcaccggaa aaaaaacaac cctttggtcg ctggtcaacc aatcaactta
    10861 aagccctgct ggcagggcaa tcagttaccg tctcttatac gcaaacagat cgctacgggc
    10921 gagtcctggg acgagtggtg acagcgaacg gcactgaggc taaccgccag caagtgctaa
    10981 aaggggctgc atgggtttat gacaggtaca acaccgataa ctcattaccg gctctgcaac
    11041 gggaagctca gacacagaag cgcggcctgt gggctgacag taatcccgtt cctccgtggg
    11101 agtggcgtca taaacaaaac tgacgcgctt cgcttgtcca acccctcctc tgcctgaaga
    11161 aaatcttcag gcaacgtcac ggtttttaat atacatggtg cgcatatggg taaattaact
    11221 ctggtagagc aggctttacg gctccataaa gaatattacg gtgaagataa ttttggtact
    11281 gtcgtaaagg attgtgatta ctatcctaaa gtggcagaga tttttataaa cagaaagaat
    11341 cgtaaaattt attcgaatct tgatatcgaa cccgttgctt caaccgtcgt tcatacggga
    11401 agtagccctg ttatggctaa agccgggagg attaaaaagc ttatgtttaa agatggttcg
    11461 ggagcatacc ggattcatct tggtcaaaat gaagtggttc atattattcg cttcattctt
    11521 aactctaaag tcagaatgga gtacgcagtt ggcacaacca aaagtatgtc gatgcttcat
    11581 aatctttgcg aagctggacg cgctaaatta a
//
