LOCUS       NZ_CP029061            32945 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain FORC_081 plasmid pFORC_081_4, complete
            sequence.
ACCESSION   NZ_CP029061
VERSION     NZ_CP029061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08660891
            Assembly: GCF_004368015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32945)
  AUTHORS   Hwang,B.K., Kim,J., Jeon,B., Choi,H., Ko,D., Chung,H.Y., Im,H.,
            Kim,H., Lee,J.-H., Ryu,S. and Choi,S.H.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Complete Genome Sequence of Escherichia coli FORC_081
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32945)
  AUTHORS   Hwang,B.K., Kim,J., Jeon,B., Choi,H., Ko,D., Chung,H.Y., Im,H.,
            Kim,H., Lee,J.-H., Ryu,S. and Choi,S.H.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2018) Food Science and Biotechnology, Seoul
            National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Gwanak-gu
            151-742, South Korea
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT v. 2.3.0
            Coverage                          :: 176
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004368015.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 06:01:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 4,996
            Genes (coding)                    :: 4,739
            CDSs (with protein)               :: 4,739
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 104 of 257
            Pseudo Genes (incomplete)         :: 160 of 257
            Pseudo Genes (internal stop)      :: 45 of 257
            Pseudo Genes (multiple problems)  :: 44 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..32945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FORC_081"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pFORC_081_4"
                     /geo_loc_name="South Korea"
                     /lat_lon="36.86590966 N 127.55281711 E"
                     /collection_date="2007-02-09"
                     /collected_by="Seoul National University"
     gene            join(30832..32945,1..35)
                     /locus_tag="FORC81_RS26000"
                     /pseudo=""
     CDS             join(30832..32945,1..35)
                     /locus_tag="FORC81_RS26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /name="DNA topoisomerase 3"
     gene            32..238
                     /gene="hha"
                     /locus_tag="FORC81_RS25480"
                     /old_locus_tag="FORC81_p495"
     CDS             32..238
                     /gene="hha"
                     /locus_tag="FORC81_RS25480"
                     /old_locus_tag="FORC81_p495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            254..715
                     /locus_tag="FORC81_RS25485"
                     /old_locus_tag="FORC81_p496"
     CDS             254..715
                     /locus_tag="FORC81_RS25485"
                     /old_locus_tag="FORC81_p496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293131.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293131.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEEAPKSRKKTLPKAPP
                     KYQFEENGETKYWSGRGRAPKPIDEALKAGRSLEDFRINKSLNGVTDEQ"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            766..1029
                     /locus_tag="FORC81_RS25490"
                     /old_locus_tag="FORC81_p497"
     CDS             766..1029
                     /locus_tag="FORC81_RS25490"
                     /old_locus_tag="FORC81_p497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072657.1"
                     /translation="MSNTSYKQIIPATDWYFRHDNVSGVAGKSTVYQLAAWALKENGEV
                     VGLVTVRDDNGRPKLVTPPPVPGDYLHKEQLTDDEKEWAKRR"
                     /name="hypothetical protein"
     gene            complement(1205..2530)
                     /locus_tag="FORC81_RS25495"
                     /old_locus_tag="FORC81_p498"
     CDS             complement(1205..2530)
                     /locus_tag="FORC81_RS25495"
                     /old_locus_tag="FORC81_p498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_024159714.1"
                     /translation="MATKKYELTKEYFFHGEFWHQLDDNKGRFSARIEYSPYHGLILDY
                     CISDSESPRTCEILYGVLNTGERCTLIGKFDFTQGNIHFGKGIIHTGRHGFPIMLFNDF
                     YAPDSKIEYCDLSLHGLQEFIHPHGFFTQLKHLEHPIFIAKGNHWTLQLVNHVSFSVIG
                     DDLLNIINCQNKAALENIIHQLKKTKELYPDAFFSIRKELVFYFRIKSSNDLGIKDHIS
                     KCWDISGLFSILLNKPTLPEEINIKFKGNDSKTPCLLTTGFEQRTIDLALREIKHQLLP
                     INRKHINLGKIFCKWFKIAERYMPLTITYQYETGFRTLHQAHTDIILFATQLEAINKTI
                     GGSKNEKYMKPINEYASLFLIQEIEMFFKKFNNKSIGENIATLRNELAHVDRKKELMNI
                     LTIGDYVKIGNYLKTIVTSYLLSDLGINNIIIEKYQAQTIQE"
                     /name="HEPN domain-containing protein"
     gene            complement(2581..3372)
                     /locus_tag="FORC81_RS25500"
                     /old_locus_tag="FORC81_p499"
     CDS             complement(2581..3372)
                     /locus_tag="FORC81_RS25500"
                     /old_locus_tag="FORC81_p499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_000501395.1"
                     /translation="MGATKDWVMQVEESRREEWIRERLSSPDLEEDSEEWQLLEKDYDE
                     YQDFLSDMAMEEYENEKWLKQHPHTEIYKIAINLLEQIKEEGKQSTSEVFIKMKIAYIV
                     TIMENCLSEMIKSVVLSHNRYVENAIRNINELKAKNISLSELINKESNANKYVQEYLSD
                     ILYHRIQLVVEIYKAVLQPKQYPRFPLKNINELMKLRHDIVHRNGKTKTTDEKIHTFNT
                     ATLNDAFKVVEEFLNNMMNLISDAVEHHENEQIARDLEDEF"
                     /name="HEPN domain-containing protein"
     gene            4099..4182
                     /gene="dqlB"
                     /locus_tag="FORC81_RS26630"
     CDS             4099..4182
                     /gene="dqlB"
                     /locus_tag="FORC81_RS26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_272056275.1"
                     /translation="MMNLLIIVLRAVLAIANALIAIQKLIE"
                     /name="dqlB"
     gene            4743..4838
                     /gene="dqlB"
                     /locus_tag="FORC81_RS26635"
                     /old_locus_tag="FORC81_p504"
     CDS             4743..4838
                     /gene="dqlB"
                     /locus_tag="FORC81_RS26635"
                     /old_locus_tag="FORC81_p504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609146.1"
                     /translation="MINLLIIVLRAVAALANALIAVLELIRQFID"
                     /name="dqlB"
     gene            4905..5084
                     /locus_tag="FORC81_RS25510"
                     /old_locus_tag="FORC81_p505"
     CDS             4905..5084
                     /locus_tag="FORC81_RS25510"
                     /old_locus_tag="FORC81_p505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000347021.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(5200..5844)
                     /locus_tag="FORC81_RS25515"
                     /old_locus_tag="FORC81_p506"
     CDS             complement(5200..5844)
                     /locus_tag="FORC81_RS25515"
                     /old_locus_tag="FORC81_p506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203199.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSLQSSGE"
                     /name="recombinase family protein"
     gene            6130..6750
                     /locus_tag="FORC81_RS25520"
                     /old_locus_tag="FORC81_p507"
     CDS             6130..6750
                     /locus_tag="FORC81_RS25520"
                     /old_locus_tag="FORC81_p507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864791.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000864791.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKSFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVSTFE"
                     /name="ParA family protein"
     gene            6802..7032
                     /locus_tag="FORC81_RS25525"
                     /old_locus_tag="FORC81_p508"
     CDS             6802..7032
                     /locus_tag="FORC81_RS25525"
                     /old_locus_tag="FORC81_p508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_000051064.1"
                     /translation="MSLEKAHTAVKKMTFGENRDLERVVTAPVSSGKIKRVNVNFDEEK
                     HTRFKAACAKKGTSITDVVNQLVDKWLKENE"
                     /name="plasmid partition protein ParG"
     gene            7049..7360
                     /locus_tag="FORC81_RS25530"
                     /old_locus_tag="FORC81_p509"
     CDS             7049..7360
                     /locus_tag="FORC81_RS25530"
                     /old_locus_tag="FORC81_p509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000360321.1"
                     /translation="MDKNVVDALKTLLEALPEEVVTEVTSKLNPSASHIPEENSKQLTA
                     KARLLNFRLTEAYEEILEVEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKIN"
                     /name="hypothetical protein"
     gene            7398..8018
                     /locus_tag="FORC81_RS25535"
                     /old_locus_tag="FORC81_p510"
     CDS             7398..8018
                     /locus_tag="FORC81_RS25535"
                     /old_locus_tag="FORC81_p510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012477163.1"
                     /translation="MDDNSTSVTSKEDVQDISLKNWLKNKKTIVLLIFTALTNVGNVLS
                     TIDIITDKTSSFYTWLGESKKFEGHWTNNTEGFIDGTPDFLLKNAGDVLIKFDLNIKGG
                     EVRGELHTDARLKMCETIEEKLKTLCTLIASHPLMIEGEKSPFSNEFDAYITEYRNGDK
                     KIVAILNMKITDDDKMTITNTMRTPESALFSTKIYAIKIITEE"
                     /name="hypothetical protein"
     gene            8043..8678
                     /locus_tag="FORC81_RS25540"
                     /old_locus_tag="FORC81_p511"
     CDS             8043..8678
                     /locus_tag="FORC81_RS25540"
                     /old_locus_tag="FORC81_p511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012477164.1"
                     /translation="MNTGITIDLTNLSEDELLDLYSMYKSANIAHQLWCRRHENIPEHF
                     SIIFVTLLERIKRVTEKNSEGVKTPDVDLDALIDTIYIGCRSMFCENPGLKNNYTLQNC
                     LRKANYHNEARVIDNILQEKKFTDSIMKDESFFSLVKLVSNKSIAHQESLSGKKREKID
                     YRYKFLNDNSNICEFQYYIFRCHRIYENIVKEYGDTLLNELKIKNNDI"
                     /name="hypothetical protein"
     gene            complement(8709..9062)
                     /gene="ddp1"
                     /locus_tag="FORC81_RS25545"
                     /old_locus_tag="FORC81_p512"
     CDS             complement(8709..9062)
                     /gene="ddp1"
                     /locus_tag="FORC81_RS25545"
                     /old_locus_tag="FORC81_p512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000876434.1"
                     /translation="MKYLVKEFINEKYTKAVNILKDNLKEHYHVFYCVRLSEILFPASE
                     YGTEQFFSDFEKINSISLPVLVFDLKEGVPVIVISLDDVSYCDVLDEMDIDFMKCDSLA
                     ELLTSDKLENLLV"
                     /name="ddp1"
     gene            complement(9168..9614)
                     /locus_tag="FORC81_RS25550"
                     /old_locus_tag="FORC81_p513"
     CDS             complement(9168..9614)
                     /locus_tag="FORC81_RS25550"
                     /old_locus_tag="FORC81_p513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074382.1"
                     /translation="MAKIYQFPQGEERAKFRKEIARERKKRFAVKTGSTFVKWLDWGWF
                     YLRLLAASVLHFIFVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSSEISQK"
                     /name="hypothetical protein"
     gene            complement(9654..10490)
                     /locus_tag="FORC81_RS25555"
                     /old_locus_tag="FORC81_p514"
     CDS             complement(9654..10490)
                     /locus_tag="FORC81_RS25555"
                     /old_locus_tag="FORC81_p514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001802123.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQISGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="replication initiation protein"
     gene            complement(11500..11631)
                     /locus_tag="FORC81_RS26640"
                     /old_locus_tag="FORC81_p517"
     CDS             complement(11500..11631)
                     /locus_tag="FORC81_RS26640"
                     /old_locus_tag="FORC81_p517"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001378418.1"
                     /translation="MALSDIALKRSVTRSERFSRFVVVGYGLTVFNAAQRFLAALLL"
                     /name="hypothetical protein"
     gene            11723..11974
                     /locus_tag="FORC81_RS25565"
                     /old_locus_tag="FORC81_p518"
     CDS             11723..11974
                     /locus_tag="FORC81_RS25565"
                     /old_locus_tag="FORC81_p518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            11964..12245
                     /locus_tag="FORC81_RS25570"
                     /old_locus_tag="FORC81_p519"
     CDS             11964..12245
                     /locus_tag="FORC81_RS25570"
                     /old_locus_tag="FORC81_p519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220562.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVISVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            12391..12714
                     /locus_tag="FORC81_RS25575"
                     /old_locus_tag="FORC81_p520"
     CDS             12391..12714
                     /locus_tag="FORC81_RS25575"
                     /old_locus_tag="FORC81_p520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001181905.1"
                     /translation="MQNYAKSVATEILRQLGGNRFIVMTGAKSFSYFDENGECGLTFRL
                     PSNFAMKGINLVKIKLDFTDTYQVKFSRVRGDEVKDISRFDNIYCDQLACLFTQETGLH
                     TVL"
                     /name="hypothetical protein"
     gene            12762..13007
                     /locus_tag="FORC81_RS25580"
                     /old_locus_tag="FORC81_p521"
     CDS             12762..13007
                     /locus_tag="FORC81_RS25580"
                     /old_locus_tag="FORC81_p521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000356542.1"
                     /translation="MDKDKIIKKNRGNYSYVIRTMDEDGDAVFHVLKYVKTIDKTKSRK
                     TVRKLIMDEKLNLASLMLLDNGILCDCLTKGDENAE"
                     /name="hypothetical protein"
     gene            12997..13212
                     /locus_tag="FORC81_RS25585"
                     /old_locus_tag="FORC81_p522"
     CDS             12997..13212
                     /locus_tag="FORC81_RS25585"
                     /old_locus_tag="FORC81_p522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001180117.1"
                     /translation="MQNKPTPEEVKNARVAAGLTLKEAADIFGYQLNSWQMKESAGKAS
                     RSLSVGEYQYLLLLANMHPSYRLVKK"
                     /name="hypothetical protein"
     gene            13305..13634
                     /locus_tag="FORC81_RS25590"
                     /old_locus_tag="FORC81_p523"
     CDS             13305..13634
                     /locus_tag="FORC81_RS25590"
                     /old_locus_tag="FORC81_p523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000866654.1"
                     /translation="MKVLTFKNDTVSVGDVFVSSWGYEQTNVTFYQVLSVHGKKTVTVR
                     EIRANSEYTDSMVGFKTPVLNDFTGECFKRQIKDFGDELAIKIEDFETAYKTLPEEKHR
                     FSSYY"
                     /name="hypothetical protein"
     gene            13676..13855
                     /locus_tag="FORC81_RS25595"
                     /old_locus_tag="FORC81_p524"
     CDS             13676..13855
                     /locus_tag="FORC81_RS25595"
                     /old_locus_tag="FORC81_p524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000439079.1"
                     /translation="MEREFSAKASLNRNIKFWFEQCGLSKERVIRCIDNWYDLAYPPSE
                     QEKAKKEAIEKLIK"
                     /name="hypothetical protein"
     gene            13925..14074
                     /locus_tag="FORC81_RS25885"
                     /old_locus_tag="FORC81_p525"
     CDS             13925..14074
                     /locus_tag="FORC81_RS25885"
                     /old_locus_tag="FORC81_p525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000003880.1"
                     /translation="MSERILSAINDVEKGGRPVFPLMPFHVFPEYMALLRKALEKKTQK
                     RTDK"
                     /name="hypothetical protein"
     gene            14087..14359
                     /locus_tag="FORC81_RS25600"
                     /old_locus_tag="FORC81_p526"
     CDS             14087..14359
                     /locus_tag="FORC81_RS25600"
                     /old_locus_tag="FORC81_p526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160399.1"
                     /translation="MTKHDFVSFVSGELRQGAVRFSLAFNSKGEIVLHWTNKAGIRVWR
                     ILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
                     /name="hypothetical protein"
     gene            14685..15230
                     /gene="ddp1"
                     /locus_tag="FORC81_RS25605"
                     /old_locus_tag="FORC81_p527"
     CDS             14685..15230
                     /gene="ddp1"
                     /locus_tag="FORC81_RS25605"
                     /old_locus_tag="FORC81_p527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_020801480.1"
                     /translation="MKKVQFRIDENQHNDLLDCLKTLYPDEPALTVAKGMKLLANALLK
                     SKAGSKDINTFFDNNDFIKTTMYLTGKQRADIERAANRHGWTLSRECRYRIQTTLENEL
                     DFFDQELLMMNRCRNSIDKIGRNFHYIIVNDQTRVLDKDGFYQDAERLTTEIFNLKNQF
                     ENYIMLCKGRTVSNKVEM"
                     /name="ddp1"
     gene            15233..16399
                     /gene="mobP1"
                     /locus_tag="FORC81_RS25610"
                     /old_locus_tag="FORC81_p528"
     CDS             15233..16399
                     /gene="mobP1"
                     /locus_tag="FORC81_RS25610"
                     /old_locus_tag="FORC81_p528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP1 family relaxase"
                     /protein_id="WP_012477147.1"
                     /translation="MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINR
                     KGASKEVVVKISGGAVTRQGIRNSIDYMSRESELPVMSESGRVWMGAEILEAKEHMIDR
                     ANDPQHVMNDKGKENKKITQNIVFSPPVLAKVKPEDLLESVRKTMQKKYPNHRFVLGYH
                     CDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLN
                     QSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFG
                     DLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRT
                     PSASKELVLNSPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL"
                     /name="mobP1"
     gene            16752..17270
                     /locus_tag="FORC81_RS25615"
                     /old_locus_tag="FORC81_p529"
     CDS             16752..17270
                     /locus_tag="FORC81_RS25615"
                     /old_locus_tag="FORC81_p529"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /GO_process="GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0140673 -
                     transcription elongation-coupled chromatin remodeling
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_012301141.1"
                     /translation="MKGQKQFYLLQCSPHFHQSVLLQLSRSGIAYYNPVIRTFYKRRDC
                     SAYRGQIMPMFPGYVFVLLDFEVVHPSCFTRMKNVYGLVSFGEYPAEVPLSVINEVKEQ
                     EKIFSMNLSLMNNSCLAKILLLADASKRGRVFADYVCNRKYDRLKNDSYKRKAETATQC
                     TLPSYRSSC"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            17200..17412
                     /locus_tag="FORC81_RS25620"
                     /old_locus_tag="FORC81_p530"
     CDS             17200..17412
                     /locus_tag="FORC81_RS25620"
                     /old_locus_tag="FORC81_p530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000125172.1"
                     /translation="MTAIKGKRKPQRNVLYLPTEVRVEVEKIAIEISFKRGRRISDSGF
                     VQYLIKKYKSQAMKELIHGADIPDE"
                     /name="hypothetical protein"
     gene            17445..18092
                     /locus_tag="FORC81_RS25625"
                     /old_locus_tag="FORC81_p531"
     CDS             17445..18092
                     /locus_tag="FORC81_RS25625"
                     /old_locus_tag="FORC81_p531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_012477148.1"
                     /translation="MLSTSTFLALAMQCAASVHPDTTHEVARVESGFNPYAIAEIIPKV
                     KRKTGDKGVVSYFPESKEAALKIVKNIELRNHRYSVGLMQITSTNFAKFGTTAEKMFDP
                     CENLKVSEKILVDCYKRGGDLVRGLSCYYSGNQETGVKPEPEFNNTSYVQRIGFSPPDN
                     KKSFIVPSVKEMIKKENKTTITPEEIIIYPQYAMRGTVSNEKETKDVEIKSE"
                     /name="lytic transglycosylase domain-containing protein"
     gene            18070..18366
                     /locus_tag="FORC81_RS25630"
                     /old_locus_tag="FORC81_p532"
     CDS             18070..18366
                     /locus_tag="FORC81_RS25630"
                     /old_locus_tag="FORC81_p532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000916182.1"
                     /translation="MLKLNLNKRYLTLSLFMAALMLCVAEPAFADDVSTKTTGFLQKII
                     DFLTDIRKPAITIIALVIGYIAIFSRQHTSWIVPLVIGIIIFIVAPYIPDWLA"
                     /name="TrbC/VirB2 family protein"
     gene            18385..21138
                     /locus_tag="FORC81_RS25635"
                     /old_locus_tag="FORC81_p533"
     CDS             18385..21138
                     /locus_tag="FORC81_RS25635"
                     /old_locus_tag="FORC81_p533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_012477149.1"
                     /translation="MSTLFKGLTRPALIRGLGVPLYPFLGMCIICVLLGVWIHEAMYAL
                     ILPGWYAIRRVTQFDERFFDLLYLRTLVKGHPLSNKRFSAVHYAGSQYNEVDISKVDNF
                     MKLKDQSSVEELIPYSSHITDNIIVTKNRDLLATWQIDGAYFECVDSEDLSILTDQLNT
                     LIRSFEGKFVTLYPHRIRCKKGVRPVFNSKIPFVNRVMNDYYESFPQSEFFENKLFLTI
                     CFKPFTTEDKVTHFFSRSKKQKDIFKEPVNEMNEICDRLNTYLSRFHSRRLGLYEDHGV
                     VYSDQLSLFQYLLSGRWQKVRVSSSPFYTYLGGKDLFFGNDAGQITASDHARYFRCIEI
                     KDYFQETDAGILDALMYLPVEYVVTSSFTAMDKQSAIKALDDQIDKLEMTDDAAKSLLA
                     DLKVGLDMVSSGYISFGKSHQTLVVFADSPERLVKDTNIVTSTLEDLGLIVTYSTLSLG
                     AAYFAQLPGNYTLRPRLSTLSSLNFAEMESFHNFFSGKEKGNTWGEKLITLRGSGNDIY
                     HLNYHMTTEHQNFFGKNPTLGHTEILGTSNVGKTVLLMTKAFAAQQFGTPESFPANRKL
                     KKLTTVFFDKDRAGEVGIRAMGGSYYRVKEGEPTGWNPAALPPTKRNIAFMKDLVRLLC
                     TLNSEPLDDYQNSLISDAVERLMQRSDRSYPVSKLRPLIQEPDDTETKRHGLKARLKPW
                     TQGEEFGWVFDNREDTFDVDNLDVFGIDGTEFLDNKVLASAASFYLIYRVTMLADGRRL
                     LIYMDEFWQWINNEAFRDFVYNKLKTGRKLDMVLVVATQSPDELIKSPIAAAVREQCAT
                     HIYLANPKAKRSEYVDGLQVRELYFDKIKAIDPLSRQFLVVKNPQRKGESDDFAAFARL
                     ELGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLEQANLI"
                     /name="VirB3 family type IV secretion system protein"
     gene            21149..21907
                     /locus_tag="FORC81_RS25640"
                     /old_locus_tag="FORC81_p534"
     CDS             21149..21907
                     /locus_tag="FORC81_RS25640"
                     /old_locus_tag="FORC81_p534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000744200.1"
                     /translation="MKKQIMAAFVASLIVISGAQAGIPVAIDANPEWAIEAGRWTERLK
                     QWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDYIK
                     NPEGALSEQAKSLLSDYKVTDTCQNLGYSGDLVRGCEATFLSQLASVEYGNKLESKLRQ
                     DNQTMKDLIDQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYRDKQRDLAEYKEKM
                     AQAAFRKQQREAVPPSYRKAYMAMKSYEDD"
                     /name="type IV secretion system protein"
     gene            21908..22135
                     /locus_tag="FORC81_RS25645"
                     /old_locus_tag="FORC81_p535"
     CDS             21908..22135
                     /locus_tag="FORC81_RS25645"
                     /old_locus_tag="FORC81_p535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000835348.1"
                     /translation="MKSIATAGLVIISVFSLSGCFEETKSVDWWLAHPKETYKKFEECQ
                     KSGSDSDNCKNVKRAHLSFERRKAVGLPIN"
                     /name="EexN family lipoprotein"
     gene            22145..23278
                     /locus_tag="FORC81_RS25650"
                     /old_locus_tag="FORC81_p536"
     CDS             22145..23278
                     /locus_tag="FORC81_RS25650"
                     /old_locus_tag="FORC81_p536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_012477150.1"
                     /translation="MGIVTGIPDGTDLSGNVTSPANAGGVSGFDSDFFQTTHEVIFNIL
                     NKSISGKLSEYSDVAYTLGKYGVSLYVLWYAFTVLARKQQTPVPDFIWNICRFYIILLF
                     VKNTGGILTSATDAIDGLKNTLAGGDPWVWMDQLWVKVIQVATLIFDKDTSTVPVAGGI
                     GALLTYVGGVLALLLCSIVFASAELTLLLLSVTAPIFIMCLMFGLLRQMFNSWLQLNFS
                     SLLVFLFAALALRAGTWQLNMALSTSIATASENNLLQTGVTSLAAGIFMAWIIWQAKSY
                     ASQIAGVGVEGAMQGAAAMGIGAGVFGASRMARGALGMGRNAGIGAWKGLRRQEDGFGQ
                     SPGITGKTANLAGQGVNIGAKKLRQAAIERAKKMYGG"
                     /name="type IV secretion system protein"
     gene            23524..24237
                     /locus_tag="FORC81_RS25655"
                     /old_locus_tag="FORC81_p538"
     CDS             23524..24237
                     /locus_tag="FORC81_RS25655"
                     /old_locus_tag="FORC81_p538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000394612.1"
                     /translation="MEFKLPGFKNKKDVTDSSVSFEEKNIALQERMNRIYKFGGIGGML
                     IGGLSLLALNAALPLKTTVVDAYLIDKVTGVAERLTSVKKENLSENEAIARYFITQYIK
                     HREGYNFFSLQHDYDYVMAYSAENVAADYNALFNSEQAPKLVYNKAEKTAMVQDNPSVI
                     ISPSSRADDKDIGAYIRFRLTIRDVATGQTRQEFWNVRLTYRIEPQVEMVSGERNNNPL
                     KFVVTSYVRDKEARG"
                     /name="type IV secretion system protein"
     gene            24242..25171
                     /locus_tag="FORC81_RS25660"
                     /old_locus_tag="FORC81_p539"
     CDS             24242..25171
                     /locus_tag="FORC81_RS25660"
                     /old_locus_tag="FORC81_p539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="WP_000783372.1"
                     /translation="MKMNKGALIMALLMAAHVCHAAVLPSGSRFDPRNQIVSYNPNNTT
                     IINSAVGYTTTLVFDEDETVISARTGFPQGWAVNKEDNLVYLEVRPVKQTVQKNNMDEN
                     GNTSSESVSVALDPENELERWRTNLFVRTTKRNYSMELNARTFRQPEKIAFVVNYQYPQ
                     ERRKEQAEIEKKRTEALARRQEEQAINRSLENAKSPRNWQYWKRVAEGSQDISPDYAYD
                     DGRYTWFGFSPLKKIPSVFVMNGMQETLTNPVIKQSGSFTAVGVPVDKRFVLRLGEQVV
                     GIENQGFGKVRLPAGDTVSPDVKKEVIQ"
                     /name="TrbG/VirB9 family P-type conjugative transfer
                     protein"
     gene            25168..26373
                     /gene="virB10"
                     /locus_tag="FORC81_RS25665"
                     /old_locus_tag="FORC81_p540"
     CDS             25168..26373
                     /gene="virB10"
                     /locus_tag="FORC81_RS25665"
                     /old_locus_tag="FORC81_p540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_012477151.1"
                     /translation="MTEQENKIPTATEIEQQLRERRQKELEQAGKTPEEEPGKPALQLG
                     IEKLKKSRKGMIILVVGFLLLAAGVSVYYIPSIIRSVSSGDEKPASQPLATGTAKRQTG
                     LSEDIDPFNTAQKKTEKPEEEKVISSEKTEPPENKQQSFSRALDVSLDGSQTGNSSSSA
                     GTSVSHTAASEPESDKKDEAKATAQTTESAPLAKITKLPYDPNLFIPEGTSIPCSLDRR
                     FVSDLAGKLECTVNSDIYSASGNVKLIERGTAAKLMYKAGSLNHGQGRVFVMAYKLRTR
                     SKPFIDIPLVDSQAAGALGEAGASGWIDTHFSERFLGAMMVGMIPDLSQAASGIAQNNR
                     DSQTDYTANSRQAFADIAREAFSNSVNIPPTLYKNQGEIITLIVGQDLDFSGIYKLKMK
                     GG"
                     /name="virB10"
     gene            26375..27406
                     /gene="virB11"
                     /locus_tag="FORC81_RS25670"
                     /old_locus_tag="FORC81_p541"
     CDS             26375..27406
                     /gene="virB11"
                     /locus_tag="FORC81_RS25670"
                     /old_locus_tag="FORC81_p541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058463.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_001058474.1"
                     /translation="MNNENRHLIYDVVNDYFYHWLNEIEGVTEIAVNRPGEIFIKVRGK
                     WQWYEQKMSYSDCLSFASTLADFHDGGSVTPEYPLRSATLPGGERVQVVIPPATEKDTV
                     SITIRKPSGIFISHDKFIKQGFYSRVSGLSGDSVMEDNISALITSGYFDRVIPESLRQG
                     KTIVFCGGTGSGKTTFANACLEYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPAEGESK
                     VITSASLLRSCFRMNPDRILMTEIRGAEAWDFLKASSSGHAGNITTVHESSPEYAVLGI
                     VQRCYMNPECQNLPFNVILRRVLSNIDIIMSIKYLDDEDFRFASGIYYKQLHFDDYFRK
                     LKE"
                     /name="virB11"
     gene            27409..29244
                     /locus_tag="FORC81_RS25675"
                     /old_locus_tag="FORC81_p542"
     CDS             27409..29244
                     /locus_tag="FORC81_RS25675"
                     /old_locus_tag="FORC81_p542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_024159713.1"
                     /translation="MSLKLPDKGQWVFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNF
                     DSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTAVALYGDAKFAS
                     DNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLV
                     RKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKD
                     LLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIG
                     SVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGS
                     FRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVE
                     VTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNE
                     IYGIEGAKTLMSAHPCRIIYAVSEEDDAAKISEKLGYITTTSKSTSKNRGRSTSQGESE
                     SEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLT
                     MKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            29241..29654
                     /locus_tag="FORC81_RS25680"
                     /old_locus_tag="FORC81_p543"
     CDS             29241..29654
                     /locus_tag="FORC81_RS25680"
                     /old_locus_tag="FORC81_p543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_012477153.1"
                     /translation="MKKILMISILVLTACSSPPEPPQVEWEKRPEVMNTQIMNWKPTSG
                     VIKSNNINSSWSKVLPDFKPENHLYDDSVFYAVAHSEKIVVRTSSFDSYWSAKDWLRKN
                     GATGVIEYQPLKRWLNNDYVEIYLSRINIQRLL"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            29651..29953
                     /locus_tag="FORC81_RS25685"
                     /old_locus_tag="FORC81_p544"
     CDS             29651..29953
                     /locus_tag="FORC81_RS25685"
                     /old_locus_tag="FORC81_p544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000820907.1"
                     /translation="MKRICKGLIVIFTVSCFIAPTYAADPCKSVFCLYGKAVGSSGGSE
                     CSSAEKDFFKNVEKKKGKIRWGKTFDLRKNFLNQCSTADPAAISLIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <30066..30248
                     /locus_tag="FORC81_RS26445"
                     /old_locus_tag="FORC81_p545"
                     /pseudo=""
     CDS             <30066..30248
                     /locus_tag="FORC81_RS26445"
                     /old_locus_tag="FORC81_p545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            30252..30827
                     /locus_tag="FORC81_RS25690"
                     /old_locus_tag="FORC81_p546"
     CDS             30252..30827
                     /locus_tag="FORC81_RS25690"
                     /old_locus_tag="FORC81_p546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012477154.1"
                     /translation="MQYALFDGMERKFLLDALEFGVLKDWKENPVKELPDIDESVHPFH
                     VCYGGYLLNPDVSDLDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKDQNIATNILNREGIFAFNGCEHRFRQ
                     PVSEDNWQQAVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     Typing          complement(9169..9614)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.881"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15234..16396
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.969"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18986..21135
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.861"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          22146..23275
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.469"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          23525..24234
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.156"
                     /coverage="100.0"
                     /evalue="2.25e-136"
     Typing          24243..25168
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          27410..29205
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.833"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ggaagctggg ttttccggca agggctaaga agtgaaaaca aagcaggaat ggctttttca
       61 gttaagaaaa tgtacatcaa gagatactct tgaaaaagtt attgagatta accgttacaa
      121 gctgccttta tcagaatcag aggcatttta ttctgccgca gatcaccgcc gtgcagaact
      181 ggtgatgaat aaactttatg ataaggttcc ttccggcgta tggaagtacg tccattaaac
      241 aagaggatta attatgagcg aactgactaa agaagatgaa tacggcatta tcagccggac
      301 tatgatgaat attcgttcat tgcgtgtgtt tgcccgtgag attgattttg agcagttgct
      361 cgaaatgcag gaaaagctca acgttgttat tgaagaacgt cgtgaagatg ctgaacgtga
      421 agcggctgaa cgagcagagc gtgaacggaa acgtcaggaa ctgcttcagt taatcgccgg
      481 agagggtttc tcaccggaag aactgcttgg tctgtccgaa gaagcaccaa aatcacgtaa
      541 aaaaacgtta ccaaaagcgc cacccaagta tcaatttgaa gaaaatggtg aaacgaaata
      601 ctggtctggt cgtggacgtg cgccaaaacc gattgatgaa gcgttgaaag ccgggcgttc
      661 tctggaagat tttcgtatca ataagagttt gaacggagta acagatgagc agtaatatgg
      721 caaggatata gttttatatc atcattttgt taaggaagaa aatccatgag taatacatcc
      781 tacaaacaaa ttatccctgc gacagactgg tatttccgtc acgataatgt ctccggtgtg
      841 gcaggaaagt caacagtata ccaactggct gcatgggcgc ttaaagaaaa tggtgaggta
      901 gttggtctgg tgacggttcg tgatgataat gggcgtccta aactggttac tcctccccct
      961 gtccctggtg attatttgca taaagaacaa ctcaccgatg atgaaaaaga gtgggcgaag
     1021 agacgctaaa ctatattcat ataaagcctc tgttctagag gctttattag catgtttagt
     1081 tgcactggag ttgataatgg cttcaatgct caaattgagg taaattgata ttgattttac
     1141 ttttccgttg ctcgtttctc gtcttattat atttaaatga caattgtaat tgtataatgt
     1201 tttattactc ttgaattgtc tgagcctgat atttttcaat aataatgtta ttgattccta
     1261 aatctgatag cagataagat gttacaattg tttttaggta gtttccaatt ttaacataat
     1321 caccaatcgt caatatgttc attaactcct ttttgcggtc aacatgtgct aattcatttc
     1381 tgagtgtggc aatattttca ccaatagatt tattgttgaa tttcttaaaa aacatctcta
     1441 tctcttgaat aagaaacaaa cttgcatatt cattaatggg tttcatgtat ttttcatttt
     1501 ttgagccacc tattgttttg tttattgctt ctaattgagt cgcaaacaat ataatatcag
     1561 tatgagcttg atgcagtgtt cgaaaaccgg tttcatactg atatgttatt gtcaatggca
     1621 tatatctttc tgctattttg aaccatttgc agaatatctt tccaagatta atatgcttcc
     1681 tgtttattgg aaggagttga tgttttattt ccctcagcgc taggtcaata gttctttgtt
     1741 caaaccccgt tgttaagaga catggtgtct tcgaatcatt tcccttgaac tttatgttta
     1801 tttcttcagg aagagtcggt ttgttcagaa gaattgaaaa taacccggaa atatcccagc
     1861 actttgatat atggtctttt attcctaaat cattagatga ttttatccta aagtagaaaa
     1921 caagttcttt tctaatggag aaaaaagcat cagggtataa ctcttttgtc ttttttagtt
     1981 gatgaattat gttctccagt gcggctttgt tttggcaatt tattatgttt aatagatcat
     2041 caccaattac gctaaaagat acatggttga caagttgaag agtccaatgg tttccttttg
     2101 cgataaatat tggatgttct aaatgtttca attgagtgaa aaaaccgtga gggtggataa
     2161 attcttgcaa tccgtgcaag gataaatcac aatattctat ctttgagtcg ggggcataaa
     2221 aatcattgaa tagcattatg ggaaatccat gccttccagt atggataatc cctttaccaa
     2281 aatgaatatt cccttgtgtg aaatcaaatt tcccaattag agtacatctt tctccagtat
     2341 ttaagactcc atataatatt tcacatgttc tggggctttc actgtcagaa atacaataat
     2401 ctagaatcag accatggtac ggtgaatatt caattcgtgc agaaaatctt cctttgttat
     2461 catcaagttg gtgccaaaat tcaccatgaa aaaagtattc ttttgttagt tcgtattttt
     2521 ttgttgccat gtactgctcc atttgtttct gagaatcctt cagaatagta aaaatactac
     2581 tcaaaactcg tcttcaagat ctctggctat ctgctcattt tcatgatgct caacagcatc
     2641 actgatcaaa ttcatcatgt tattaagaaa ctcctctaca actttaaacg catcattgag
     2701 cgtagctgta ttgaatgtat gtatcttttc atccgttgtt ttcgttttcc cgttacgatg
     2761 aactatgtca tgcctcagtt tcatcaattc attgatattt ttgagtggaa aacgtggata
     2821 ttgcttaggt tgcaggactg ctttatatat ttcaactacc agttggattc tatgataaag
     2881 aatatctgaa aggtactcct gtacatattt gtttgcatta gactctttat ttatcaactc
     2941 agacagagag atattctttg ctttcagttc atttatgttt cttatggcat tttctacata
     3001 tctgttatgt gacaatacaa cgcttttaat catttcgcta agacagtttt ccattattgt
     3061 cacaatgtat gcgattttca tttttatgaa aacttcactt gtagattgtt ttccctcttc
     3121 ttttatttgt tctaaaaggt ttattgctat tttataaatt tctgtatgtg gatgctgttt
     3181 taaccatttt tcgttttcat attcttccat agccatatca gaaagaaagt cctgatactc
     3241 gtcatagtcc ttttcaagta actgccattc ttcagaatcc tcctcaagat ctgggctgga
     3301 aagccgttct cgtatccatt cctcacggcg actttcctcg acttgcataa cccaatcttt
     3361 tgtagctccc acaataccct ccttatgttc attgctaaat atcaatagtc actttatcgg
     3421 agactacgtg aaaaatcttc tcctttcccc ctgctttttt atcagatttc acgcagcgta
     3481 ccgccgctac tgtatccatc aaaaaaactg atagcttttt aatcatcttt tgtgctgcaa
     3541 cttactgaaa aaaggattaa tggaaacgcc gataacggca ttgatctgtt ggccaacgcg
     3601 accggcagca ttggacttaa caaaaaaact gaaagaaaat cccccaaatg aagggaaaaa
     3661 acggaagaac agtcgaacga caggcacaaa aaaacccgac tggtaatcgg gctttctcgt
     3721 gttttccagt cctgagttgg tggctctgac tggaggaaga tactctacat ccaacatcca
     3781 caatattatg gcactagaca ccagttgtct agtttgactt cagattgtct gtcaatggcc
     3841 atttctttaa atgaatttta tgcgtgcatc tgtataatct ttgtgttggc gctgtacagt
     3901 gtgcctccct tgcttctaac ccctgcgcta tctggggatg aacgaattaa agtcctggct
     3961 gaacaaacag gtggtgctgt gttcagtgct ctggcagggg gaaggtactc tacatccaac
     4021 aatgtgagag agccgttgtg acaggcgatg tatgagcaag caacggtgac ttcctccagt
     4081 atcaacctag agaaacggat gatgaatctg ttaatcatcg ttctcagggc tgtgctcgcc
     4141 attgcaaacg cgctgattgc cattcagaaa ctaatcgagt gattgatggc ttaactaagg
     4201 gagagacgtt taccgcttcc tgccctttaa caaaggagtg tttatgagtt ttgaacaatt
     4261 atttacacta acgacccaac gtcgttgggt ttgccgcaga aaatttattc atttaaaata
     4321 gatctaaatg aagcgggaaa ctgcagacgt aaaaaaaccg actggtgagg tcggcttttt
     4381 tacagttccg gtacgagctg gtaactcgcc cggaagagaa actctctaca actgacaaca
     4441 gtatgataat ccgtgaagcc tgattatgca atatgacagg cggtgactac gcgttaacgg
     4501 tttgcttgaa agttagataa acgtataatc agagggttag tgctgtggtg agtctttccc
     4561 tgcattccgc taaatgcggc atgaacagct tcaaagtcct gacaggtaat acaggtggtg
     4621 ctggttatct gtgctctggt aggagtgaga ctctctacaa ctgacaagac gaagagaagc
     4681 cgtggtgaca ggcagcgtaa gagcaagcca cggtgtcttt actccattta tggagaaaac
     4741 ggatgattaa cctgttaatc atcgttctga gggcggtagc tgcgcttgca aacgcgctga
     4801 ttgcagttct ggaactgatc cgtcagttca tcgattgatg actgcggaaa cgtaactaag
     4861 ggcaggaagt gcaccgcttc ctgcccttta acaaaggagg tgttatggat tttatgagca
     4921 ttttggggcg ctgtttactt cttcctttcc ctgttgcgat gtttgtcggt tgcctgtttc
     4981 caggcatcga tgatcgtttt actggaatgt tcatgagttt tattgtcggt tgtctatcgt
     5041 ggtatatcac caagccaaaa ccgcaaaagg ctgaggtcgc gtaagcggcc ttttattttg
     5101 atacgcatta gttacaacat tcaatttgct ttttaaaaat atccacttca gcacctccct
     5161 cataaataac gtcaaccagt ttattgagct caccctaccc tactcaccgg aggattgcag
     5221 ggatttacgt tttattgcgc tactgattgt gccgcggctg caccctagta ccttctgtac
     5281 ctgtgtccag gagctgccac tttctatcaa cctgataatt gcatcatgtc tggcttgatt
     5341 aggtttacgc cctttatatt tgccgtcttt ccttgctttc tctatcccct gtttctggcg
     5401 ttcccgccgt tgttcatagt cccttctagc aacagcggcc agcatatcca gcagcatatc
     5461 gttaatggct gcaaacaacc ggctgtcaaa ctcactcata ccagaattaa tccaggtcgt
     5521 cggcacattc acggccataa cccggatatc tttctgacgg atcattttct tcagcgtatt
     5581 ccagtcttcc cctgacagtc gggaaagtct gtccacatcc tcgataagca aaatgtcatt
     5641 ttgctggcaa tctctcagaa gacggaagag ttccgggcgg tcaagacggg agccggattc
     5701 attctcaata taatagctgc aaatgatcag gcctctttcc ctggcaaaag cctcgatagt
     5761 ttccagggca cgcgaagcat cctgttctgc tgttgaagct ctcagataag cgcggacaaa
     5821 gtttgttggt gcatttgaag tcatattaca gaaccagttc gcataaggtc acaccactat
     5881 acccaaaatg aaccagaaag tggatcgaca ggaaaggcac actctaaaag aaccgaaaaa
     5941 acaaataatg agtaaatgtg acatcgtcac ccatactcat aaactcacat gctcatatac
     6001 acaacaaact caaatgagta tgtgctcttt aactcataaa ctcatattaa cctttactca
     6061 tgcaaagagt atggtaaact caaaaactca caagagtaat tgagtagata cacaattgag
     6121 ggtttgagta tgaaagtaat ctcatttctg aatccgaaag ggggttcagg taaaacaact
     6181 gccgtaatca acatagccac tgcgttgagc agaagcggat acaacattgc tgtggtagat
     6241 acagatccac aaatgagcct gacgaactgg agcaaagcgg gcaaggcagc atttgacgta
     6301 tttacagctg catctgaaaa agacgtctat ggaatccgaa aagatctggc ggactatgac
     6361 tttgctattg tggacggggc aggttcgctc tcagtaatca cctccgcagc cgtcatggta
     6421 agcgatctgg taattatccc tgttacaccc agccccctgg atttctccgc agcaggaagc
     6481 gtcgttactg ttctggaagc acaggcttac agccgcaaag ttgaagcccg ctttctgatc
     6541 acccgtaaga tagaaatggc aaccatgctc aatgtgctga aagaaagtat caaagacact
     6601 ggtgttaaat ctttccgtac ggccattaca caacgtcagg tttacgtgaa atcaattctg
     6661 gatggtgaca gcgtgtttga atccagtgat ggcgcagcaa aaggtgaaat agaaatcctt
     6721 acaaaagaga tagttagcac atttgagtaa ttactcattc actcatataa tcaaataagt
     6781 ataacaaccg gagtaacctt aatgtcactt gaaaaagcgc atacggcagt aaaaaaaatg
     6841 acctttggtg aaaacagaga tctggaacga gtagtaacag caccagtatc atctggaaaa
     6901 atcaaacgtg ttaacgtcaa ttttgacgaa gaaaaacaca cccggtttaa ggctgcatgt
     6961 gccaaaaaag gtacatcgat cacagatgtg gtgaaccagc ttgtagataa atggctcaaa
     7021 gagaacgaat aatatctgag gatatatcat ggataaaaac gtcgttgatg cgctgaaaac
     7081 gctattagaa gcgttaccgg aagaggtggt aacagaagtc acatcaaaac taaatccttc
     7141 ggcaagccat attcctgaag aaaacagtaa gcaattgaca gcaaaagcaa gactactgaa
     7201 tttccggcta accgaagcct atgaagaaat cctggaagtc gaagctatca gaacaggcca
     7261 gagcaaaact accgttctaa aggcagcact ggcgatgtac aacagccagg atgaaaacat
     7321 aaagaatcac tggctacttg agtctgcaaa aattaactaa ttcaggatat agattattag
     7381 taggagttcg ggcaataatg gatgataact ctacgtcagt aaccagcaaa gaagatgtgc
     7441 aggatatttc tttaaaaaac tggctaaaga ataaaaaaac cattgtatta cttatattta
     7501 ctgcattaac aaatgttggc aatgtccttt cgacgattga tattatcaca gataaaacca
     7561 gttcttttta cacatggctt ggggaatcga aaaaattcga aggccattgg accaacaata
     7621 cagaaggatt catagacggc actcctgatt ttttactcaa gaatgcaggc gatgtcctca
     7681 taaaatttga cctgaacatc aaaggtggcg aagtgagggg agagcttcac accgacgcac
     7741 gactaaaaat gtgtgaaact atagaagaaa agttaaaaac tttgtgtaca ttaatagcct
     7801 cacatccact aatgattgag ggggaaaaat caccattttc caatgaattc gatgcctata
     7861 ttactgagta tagaaatgga gataaaaaaa tagtcgcaat attaaatatg aaaattacag
     7921 acgatgacaa aatgacaata acaaatacaa tgagaacacc agaatcagca ttattttcaa
     7981 ctaaaatata tgctataaaa ataataaccg aagaataact taataacaaa aaaggtatta
     8041 aagtgaatac tggaataaca attgatttga ccaacctttc agaagatgaa ttgcttgatt
     8101 tatattcaat gtataaaagt gcaaacatag cacatcaact atggtgcaga cgccatgaaa
     8161 atataccaga gcatttttcg ataatattcg tgacgctttt agaacgaata aaaagggtta
     8221 cagaaaagaa ctcagaaggg gtaaaaacac cagatgtaga cctggatgca ttaattgaca
     8281 ccatttatat tggttgtcgt tcaatgttct gtgaaaaccc tggcttaaaa aataactaca
     8341 ctctgcaaaa ctgcctgagg aaagccaatt atcacaacga agctagagtg atagataata
     8401 ttttacagga aaaaaaattc acagattcca tcatgaaaga tgagtcattt tttagtctgg
     8461 tgaaattagt ttccaataag tccattgcac accaggagag cctttcagga aaaaaacggg
     8521 aaaagataga ctatcgatat aaattcttaa atgacaattc aaatatctgt gagtttcagt
     8581 attacatttt tagatgtcac cgtatttatg agaatattgt gaaagaatat ggggatacat
     8641 tactgaatga gcttaaaata aaaaacaatg atatataaag aatgccccca tatgaataga
     8701 aggaagaatc atactaataa attttccagc ttatcgctgg taagcaattc tgccagcgaa
     8761 tcacatttca taaaatcaat atccatttcg tccagaacat cacagtagct gacatcatca
     8821 agactaatga caatcacagg aacaccctct ttgagatcaa acactaagac aggtaatgaa
     8881 atggagttta tcttttcaaa atcactaaaa aactgttcag ttccatattc actggcagga
     8941 aaaagaatct cacttaatct cacacaataa aaaacatgat agtgttcttt aaggttatcc
     9001 tttaaaatat taacagcctt agtatatttt tcgttaataa actctttgac caaatatttc
     9061 ataaaactct ccaacaagaa ccgactgtag gtcatcgggc aaacgttgcg gaatggcgtc
     9121 agagacgtca ttttgcggcg tttgccctat cctgcatcgc agtggcatca tttctggctt
     9181 atctcgctac tgttctcggt gctttcgtcc gcaggttcgg gaacaagtaa cttgtgccag
     9241 ggcatagttt tgttgagcag ctccatcaga ggctcagcca gcagtcccaa aatccaggtt
     9301 gccgtaatca ccggtatagt gagattttgt ggcgtccata actggtgatc gagatgatac
     9361 caggtgatga cacacaaaat accacccagc cagaacacag cccatttaaa cgcaccaaga
     9421 atggctaaca caaaaacaaa gataaaatgt aaaacactcg cggcaagaag acgaagataa
     9481 aaccatcccc agtcaagcca cttaacaaaa gtgctccctg ttttcactgc aaaacgtttc
     9541 ttacgctccc tggcgatttc tttcctgaat ttagcacgtt cttccccctg agggaattga
     9601 tagattttag ccatgagtta cctcatttcg atctcaaaac gaagtttatg caattatcct
     9661 ctgaaaccgt atggtattct gatatctagg ccttcacttt ctttatctat tttataagaa
     9721 aaccttagct ttgatgctct tcttgcgact ctctcaacga ttttaacttc gagatgatta
     9781 atctctgtat attgatttat tttttcaatg tttttttgca gaaaaacttt attaaagtcc
     9841 ttaaaattct gataaaggta aactttttcg ccattatcta ttctgaataa ttcaagttca
     9901 tcctttaata catctacttc aatgtcaaaa aatgtctttt taccactgct gtattgtttc
     9961 ctgataaact gataaagcat atttgaattt tgatcagcaa gtcttacaga cgatatcaaa
    10021 acctgagtag tgtaagaatc ctttaacatc gtgatatatg gctcaatgac atgtgaaaaa
    10081 gcaatatcaa tatatccttc actttcaaca taatcacaaa acgcagtcag gttcataagc
    10141 ctgactgcat cagaaggtaa tttacctctc ttcttcgtat gtccagagat ttgattgccc
    10201 tgagctgtga gaagttgatt tcgaggaatg cgaataacac tggcctgaag ttcttctgcc
    10261 ccctctttca actgccggta agcacctgtt acatcaatat cagcgataaa tgcatattcc
    10321 cgagccgtaa tacgaaaggt tgccccctca gataactctt cacgtgaatc aatctgcgcc
    10381 atagccataa acaaaattct tctggcagac aaaggcaacg atgagaatgt gctgttaatt
    10441 tcatttctgt ggcgaatttt tgttttcttt gtaacgctca gtaagttcat acattacctg
    10501 tggtttttaa gttctaatgg tgaaatcata accacaaagg cggaaatatg taaagtggtt
    10561 tatccacctt atgtatttcc accaaaacac ggatcacatg aaagaatttt tatcattaat
    10621 attcaatatg atagaaatgc gcaggtatcc accttttaac gtcatacgtt agattagagc
    10681 actataaaga actaaagaaa tgaagataca aagatacgaa tatctgaata aaaacaggaa
    10741 aatgcgtacc catccacctt tcagtgcgta cccatccacc tttcagtgcg cagccatcca
    10801 cctttcatga aaattacagt gcgtacccat ccacctttca gtgcgtaccc atccaccttt
    10861 cattttgagg cgacaaaaaa caacacctat cttaatgaaa ttattgataa aaaaactggc
    10921 acaatgtttg cagaataagt taaagtaagt taaagatctt aaagtagatc taccgatcta
    10981 catttaagat cttaaaaaca agaagtttaa taagcaaaaa aacacaaaaa atctgtggat
    11041 aactttgctc aaaacaaaaa attcacacta cctgtggata actttgcgta aacccggagg
    11101 acagatcact ctgaaccaag acaaccacac ataaaaacaa tatgctcact ttttaaccac
    11161 tgcgcgtaat tgcctgatgt tatccttgtg ctgttccgct tcctcgctca ctaactcgct
    11221 acgctcggtc gttcggctgc ggcgagcggt gtctcctttc tcaaaaccag aagagttcag
    11281 atcatacgat ccgttgattg atcctttttg gatctttcac tgagaagccc tgaatcgctt
    11341 tctgtcgctt cgttcgatac aaccatggtg aaaccatgcg aaatcgaaaa aatcgctcag
    11401 aatgcgatac agcgcgtttt agagggtatt tacaaaacag tcgttgcctt gaggaaattt
    11461 tgatcaagat tgaactccta tccggatcat ggcaacagat cacagcaaca aggccgctaa
    11521 aaagcgttga gcggcgttaa acaccgtcaa accgtagccg actacaacga aacgggaaaa
    11581 acgctcagaa cgcgttacag agcgttttaa ggcgatgtcg gaaagagcca tatctcacct
    11641 gttctgatgt ttttctcaac atgagcactt ttaagagtgc ttttaaagat gctattatgc
    11701 caatgagaca agcggagggc ttatgagcta tcaaattctg acaaccacag cggccagtat
    11761 tactgacctg aaaaaaaatc ctatgggaac cgtagctgaa ggtgaagggg acgctgttgc
    11821 gatcctgaac cgaaacgaac cggcgttcta ttgcgttcca ccaaaacttt acgcctacta
    11881 tcgggaactc gctgaagatg ctgagttaaa cgctgttgct gatgagcgca tgaaaaaccc
    11941 ggaaattgtg aaggttaacc tggatgacct atgaactggc ttttgaccgt agagcactga
    12001 aggaatggca gaaactcggc cacaccatcc gtgaacaatt caaaaagaaa ctggcagaac
    12061 ggctggaaaa tccacgcgta cccgcagccc ggttacatgg tcatgctgat cgctataaaa
    12121 tcaaacttcg tgcatctggc tacagacttg tatatcaagt cattgatgag aaagtcgttt
    12181 tacttgttat ttccgttgga agaagggaaa gcagcgaagt ctatcagatc gcagatttgc
    12241 gctaagatta atcatcaaag gattaactgt ttactgtcag aacaagagca ttaaaaattt
    12301 gccacacctg caaatgcagg tataataaaa cacaagaaag ggaaacaggt tctctttcat
    12361 ccagagccca aagtgggcgg gaggttaaaa atgcaaaact acgctaagtc tgtagccaca
    12421 gagattttac gtcaacttgg tggtaatcgt tttattgtta tgactggtgc taaaagtttt
    12481 tcttactttg atgaaaacgg tgagtgcggg ttaactttcc gtttgccgtc caattttgca
    12541 atgaaaggca tcaacttagt aaaaattaaa ctggatttta ctgatacgta ccaggtgaaa
    12601 ttttctcgtg tacggggtga tgaagttaaa gatatttcaa gattcgataa tatctattgt
    12661 gatcagttag cgtgtttatt tacacaagaa acagggttac ataccgtgtt atagataaaa
    12721 aggggcatta agcccctttt ttagttatta tgaggaataa tatggataaa gataagataa
    12781 ttaagaaaaa cagaggtaat tactcatatg taatcagaac gatggatgaa gatggggatg
    12841 cggtttttca cgtcttaaaa tatgttaaga cgattgataa aactaaaagc aggaaaacag
    12901 taagaaaatt gataatggac gaaaaactca acctggcatc attgatgctt ctggataatg
    12961 ggattttgtg tgattgtctg acaaaagggg atgaaaatgc agaataaacc tacacctgaa
    13021 gaagtaaaga atgcacgggt tgcggcaggt cttactctta aagaagctgc tgatattttt
    13081 ggttatcaac tgaattcctg gcagatgaaa gaaagtgcag gtaaggccag tcgttcttta
    13141 tctgttggtg aatatcagta tttattgcta ttagcaaata tgcatccgtc ttacaggctg
    13201 gtaaaaaaat aacttgctat acctgcaaat gcaggtataa taaaacacaa gaaagggaaa
    13261 caggttctct ttcatccaga gcccaagctg ggcgggagct aaaaatgaaa gtattaactt
    13321 ttaaaaatga tactgtctct gttggtgatg tctttgtatc atcctggggc tatgagcaaa
    13381 ccaatgtaac cttctaccag gttctttctg ttcacggtaa aaaaaccgtc accgttcgcg
    13441 agattcgcgc taattcagaa tataccgatt caatggtcgg ctttaaaact cccgttttaa
    13501 atgattttac tggtgaatgt tttaagcgcc agataaaaga ttttggtgat gagctggcaa
    13561 tcaaaatcga agattttgaa actgcgtata aaactctacc ggaagaaaaa catcgatttt
    13621 cttcttacta ctgattaatc agggggatat tccccctttt agtaaaggtc tgaaaatgga
    13681 aagagagttt agcgcaaaag catcattaaa ccgaaatata aaattttggt ttgagcaatg
    13741 tgggttatct aaagaaagag ttattcgttg tattgataac tggtatgacc ttgcataccc
    13801 accatcagaa caggagaaag caaaaaaaga agcaattgag aagttaataa agtaaagata
    13861 aagatggtat caggttgtgg ggcaacctga taccgtgaca cctgattcta ttgcgaggaa
    13921 taaaatgtcg gaacgcatat tatcagcaat aaatgacgtt gaaaagggtg ggcgtccggt
    13981 tttccctttg atgccattcc atgtctttcc tgagtatatg gcattactca gaaaagcact
    14041 ggaaaaaaag acacaaaaga gaacagataa ataaaccgga ggctttatga caaaacatga
    14101 ttttgtgtcc tttgtcagtg gagaattgcg gcaaggcgct gtacgctttt ctcttgcttt
    14161 taacagcaaa ggagaaattg ttctgcactg gactaataaa gcaggaatac gagtatggcg
    14221 gatattaagt ggtaatcgtg gcaaaaaacc aagcaaagct aaccttgaaa gaatgagtaa
    14281 cttccggcgc tggctttttg atgcccgtca gggcatggaa ggctacactc agcaacctga
    14341 acagagcaac cttagctgaa tagccgaagc cctcccgtat ttcgggaggt gctttctgat
    14401 atattgcgcg ggactgcgct tgcgcttcct gccagaccgt tcgcggagcg aacctgacgg
    14461 cactgtcctt accgcattga tcgggtttcc ggtcgcagac gtacgtatgt tcagccatga
    14521 caacatcagc ctttttgcat ttttgttgat gctcaagcac aatttatgag cggtgccaga
    14581 ttcactgcga tgcaggatag ggcaaacgcc gcaaaatgac gtctctgacg ccattccgca
    14641 acgtttgccc ggtgacctac agtcggttat tgtcggagaa ttttatgaaa aaagttcaat
    14701 tcagaattga tgaaaatcag cataatgatt tgctggattg tcttaaaact ctttatccag
    14761 atgaaccagc tttaacagta gctaaaggca tgaaactttt agcaaatgct ttattaaaaa
    14821 gtaaagctgg cagtaaggac ataaatacgt tttttgataa taatgatttt atcaaaacaa
    14881 cgatgtactt aacaggtaaa caaagggctg atattgaaag agctgctaat cgtcacggat
    14941 ggacgttatc acgagaatgt cgttaccgca tacagacgac acttgaaaat gaactggatt
    15001 tctttgacca ggaactgctg atgatgaatc gttgccgtaa ttcaattgat aagatcggtc
    15061 gtaatttcca ttatatcatt gttaatgatc agaccagggt tcttgataaa gatggtttct
    15121 atcaggatgc ggagcgtctt acaacagaaa tttttaatct taagaatcag tttgagaatt
    15181 acattatgtt atgtaaaggg agaactgttt caaataaagt ggagatgtaa ttatgggcgt
    15241 ttacgttgat aaagaatatc gtgttaaacg aaagtcatca gaaaatggtc gtaagtcagc
    15301 tttcgctcac aaagtcaaaa atggtggaaa gaactatagc cgcaatgttc aggaacgtat
    15361 caaccgcaag ggtgccagta aggaggttgt tgtcaaaata tctggaggtg ctgttactcg
    15421 tcaggggatt cggaacagta ttgattatat gagccgtgag tcagagctac cagtgatgag
    15481 tgaaagcggt cgggtatgga tgggtgccga aattctggag gctaaagagc acatgataga
    15541 tcgtgctaat gatcctcagc atgtgatgaa tgataaaggt aaagaaaata aaaaaatcac
    15601 acagaatatt gtcttctcgc ctccagtttt agcgaaagta aagcctgaag atttgttgga
    15661 gtctgtcagg aaaacgatgc agaaaaaata tcctaatcac cgttttgttc ttggatacca
    15721 ctgtgacaag aaagaacatc ctcacgttca tgttgttttt cgtatccgag ataatgacgg
    15781 taaacgcgct gatatcagga aaaaagattt acgggaaatt cgtacaggtt tttgtgaaga
    15841 gttgaagtta aaaggttatg acgttaaagc gacccataag caacagcatg gacttaatca
    15901 gtctgttaaa gatgcacata atacagcacc aaaaagacag aaaggtgttt atgaggttgt
    15961 tgatattggc tatgaccatt atcagaacga taaaacaaag tctaagcaac attttataaa
    16021 gctaaagact cttaacaagg gggttgagaa aacatactgg ggggctgatt ttggggactt
    16081 atgttcgcgg gaaagtgtta aagcaggtga tcttgtcagg ctgaagaaac ttggtcagaa
    16141 agaagtaaaa atcccggcgc tcgataaaaa cggtgttcag catggctgga aaacggttca
    16201 cagaaatgag tggcagttag aaaatctggg ggttaagggc gtagacagaa caccttcagc
    16261 cagcaaagag ctggtactta acagccctga tatgctgctg aagcaacaac agcgaatggc
    16321 gcagtttacg cagcagaaag catccacgtt acagtcagaa cagaagctga aaacagggat
    16381 taagtttttg ggcttataag atttaatgta ttgattatgc gagaaaaata tttattttag
    16441 ttcgcttgct catcttttca ccttaaaaac agatcgaaat tctgcgtttt agctatcatt
    16501 cgatctatcg atctaaaaaa cagatctgca aaagatgaat attctgtgtt tcattgcata
    16561 catggaataa atgaaagtaa aattgattta tcaagatcga aagatctgtt ttatgtgttg
    16621 aacttagcat aagccacttg tagtataaac ttcgcgcaat agtaaaaagt ctctcttcat
    16681 aggggcgtga tttctcacaa gcaaccacgg ttggcttgtg ttttgtcaca tctgtatatc
    16741 ggggattgag tatgaaagga cagaaacagt tttacctgct tcagtgttct ccgcattttc
    16801 atcagtcggt tctgttgcag ttatcccgga gcggtatcgc ttactacaat ccagttatac
    16861 gcactttcta taagaggcgt gactgtagtg catacagggg acaaatcatg ccaatgtttc
    16921 ccggttatgt attcgttctt ctggattttg aggttgtaca tccatcctgc tttacccgga
    16981 tgaaaaatgt ttatggactg gtcagttttg gtgaatatcc tgctgaagtt cctctgagtg
    17041 tcataaatga agtaaaagag caggagaaga ttttttcgat gaatttaagc ctgatgaata
    17101 actcctgtct ggcaaaaata cttctccttg cagacgcttc aaagcgtgga cgagtctttg
    17161 ccgactatgt gtgtaacaga aaatacgaca ggttaaaaaa tgacagctat aaaaggaaag
    17221 cggaaaccgc aacgcaatgt actctacctt cctacagaag ttcgtgttga agttgaaaaa
    17281 attgcaattg aaataagctt caagaggggg cgacgtatct ctgattctgg ttttgttcag
    17341 tatcttatta aaaaatacaa atctcaggcg atgaaagaac ttattcatgg ggctgatatt
    17401 cctgacgagt aatattgtcc caatgttctg aatgtggaat atttatgtta tctacttcta
    17461 cttttcttgc gcttgccatg caatgcgctg ccagcgttca tcccgacaca acgcacgaag
    17521 tcgccagggt tgaatcaggt tttaacccat atgcgattgc ggaaataata ccaaaggtta
    17581 aacgtaaaac tggtgataaa ggcgtagtat cttactttcc tgaatcaaag gaggcagcac
    17641 ttaagatcgt taaaaacatt gaattacgga atcatcgtta ctctgtagga cttatgcaaa
    17701 taacgagtac caattttgca aagttcggta caacagcaga gaaaatgttt gatccatgcg
    17761 aaaatcttaa ggtatcagaa aaaatactgg ttgactgtta taaacgaggt ggcgacttag
    17821 tgcgtgggct gagttgctat tattctggca atcaagaaac aggagtaaag ccagaacctg
    17881 aatttaataa tacaagttat gtacaacgta taggatttag ccctcctgat aataaaaaaa
    17941 gttttattgt tccctctgta aaggaaatga ttaaaaagga gaataagacg actatcacac
    18001 ctgaagaaat cattatatat cctcaatacg ccatgcgtgg cactgtatca aatgaaaagg
    18061 aaacaaaaga tgttgaaatt aaatctgaat aaacgttatt taacgctttc tctatttatg
    18121 gctgcgttga tgctttgtgt tgcagaacct gcttttgcgg atgatgtgtc tacgaagaca
    18181 actggttttt tacagaaaat aattgatttt ttaacggata ttcgtaaacc tgcaattaca
    18241 attattgctc ttgttattgg gtatattgcg atattttctc gtcaacatac ctcatggata
    18301 gtcccacttg ttatcggaat tatcatcttt attgttgcac catatattcc tgactggctt
    18361 gcgtaattaa aggatgggcg cactatgagt actcttttta aaggtcttac gcgccctgct
    18421 ttaataaggg ggctgggcgt tccgctctac ccctttcttg gaatgtgcat tatttgtgtt
    18481 ctgcttggtg tctggattca tgaggctatg tatgccctta ttcttcctgg ctggtatgcc
    18541 atcaggcgtg taacacagtt tgatgaacgc ttttttgacc ttctgtatct gagaactctt
    18601 gtcaaagggc atcctttatc aaacaagcga ttcagcgcag tccattatgc ggggagccag
    18661 tacaatgaag ttgatatttc aaaagtggat aactttatga agctgaaaga ccagtcttct
    18721 gttgaagagt taattccgta ctcttcacat atcactgata atattatcgt tacaaaaaac
    18781 cgggatttgc tggcaacctg gcagatagac ggtgcttact ttgagtgtgt tgattctgaa
    18841 gatttgtcaa ttctgacaga tcagcttaat acgcttatac gtagctttga agggaaattt
    18901 gttacgcttt atcctcatcg tatcaggtgt aaaaagggcg tcagaccagt atttaacagt
    18961 aaaattcctt ttgtgaacag agtaatgaat gattattacg agtcattccc tcagtctgaa
    19021 tttttcgaga ataaattatt tctgacgatt tgttttaaac cttttactac ggaagataaa
    19081 gtaacacatt tcttttcacg cagtaaaaaa caaaaagata tctttaaaga gcctgttaat
    19141 gaaatgaatg aaatttgcga caggttgaat acctatctgt cccgttttca ttcccgacgt
    19201 cttgggcttt atgaagatca tggggttgtt tattcagatc agttatctct gttccagtat
    19261 ctgctttctg gtcgatggca aaaggtcagg gttagcagta gtccgtttta tacatatctg
    19321 ggaggaaaag acctgttctt tggtaatgat gccggacaaa ttaccgcgtc agaccatgcc
    19381 cggtattttc gttgcataga gattaaggat tattttcagg agacggatgc cggtattctg
    19441 gatgctctga tgtatctccc cgttgagtat gtcgtgacat cgtcctttac tgcgatggat
    19501 aagcagtcag cgattaaggc gctggatgat cagatcgata agctggaaat gacagatgat
    19561 gctgccaaat ctttgctggc agatctgaaa gtcggactgg atatggtttc cagtggatat
    19621 atttctttcg gaaaatcgca tcagaccctg gttgtctttg cggattcacc ggagcggctg
    19681 gtgaaagaca ccaatatcgt gacttccact ctggaagatt tggggctgat tgtcacttat
    19741 tcaacactga gtcttggcgc agcttatttt gctcagctac caggaaatta tacgcttcgc
    19801 cctcgtctga gtaccctcag tagtcttaat tttgccgaaa tggaaagttt tcataatttc
    19861 ttttcaggaa aagaaaaagg aaatacctgg ggggaaaaac tgattactct tcgggggtca
    19921 ggtaatgata tctaccatct gaattaccat atgacgactg aacatcagaa tttcttcggt
    19981 aagaacccga cgctggggca taccgaaatt ctcggtacgt ctaacgtggg taaaaccgta
    20041 ttactgatga caaaagcatt tgccgcccag cagttcggta cgccggaatc attccctgca
    20101 aacagaaaac tgaaaaaact gaccacggtt ttttttgata aagaccgggc aggtgaagtc
    20161 ggtatacggg caatgggggg atcttattac cgggtgaagg agggagagcc gacaggctgg
    20221 aatcccgccg cactgccgcc aacaaagcgt aatatcgctt ttatgaagga cctggtgagg
    20281 ctgctttgta ctctcaacag tgagccgctc gatgattacc agaacagcct gatttcagat
    20341 gcggttgaac gtcttatgca acggtcagat cgctcttatc ctgtcagtaa actacggcct
    20401 cttatccagg agccggatga tactgaaacc aaacgtcatg gacttaaagc ccgtcttaag
    20461 ccgtggacgc agggggaaga gtttggctgg gtgttcgaca atcgggaaga cacgtttgat
    20521 gtcgataacc tggatgtttt cggtattgat ggaacggagt tcctggataa taaggtgctg
    20581 gccagtgctg cttcattcta tctcatctat cgggtcacca tgctggccga tggtcgcagg
    20641 cttcttatct acatggatga gttctggcaa tggatcaata acgaagcgtt cagggacttt
    20701 gtttacaaca agctgaaaac cggacgtaaa ctcgatatgg tgcttgtcgt agccacacag
    20761 tcaccggatg aactgattaa atcacccatt gcggcagcgg ttcgtgagca atgcgccact
    20821 catatctatc tggcaaaccc gaaagccaaa cgtagtgaat atgttgatgg tttgcaggtc
    20881 agggagcttt attttgacaa aattaaagct atcgatccgc tgtcccgcca gttccttgtt
    20941 gttaagaacc cacagaggaa aggtgaaagt gatgattttg ctgcttttgc cagactggag
    21001 ctgggaaaag cagcgtatta cttaccggtt ctcagtgcat caaaacccca gttagaactg
    21061 ttcgatgaaa tctggaaaga aggaatgaag ccggaagagt ggcttgatac ctatctggaa
    21121 caggcgaacc tgatttgagg aatcaccaat gaaaaagcaa attatggcgg cattcgtcgc
    21181 ttcactgatt gttatttccg gcgctcaggc agggatacct gttgccatcg acgccaaccc
    21241 tgaatgggcg attgaagccg gacgatggac agaacgcctt aagcaatggg cggaaacggt
    21301 aaaacattac gaaaatcaga taaatgcgta caaacaggag ctgctgtcaa aaacgggtat
    21361 ccgtgatgtg cagggactgg tgcagtccgc acagtcagtg agtcaggaac tgatgcagat
    21421 ttatgatcag gggaatgctt ttattgacga ttacattaaa aatcctgaag gggcgttatc
    21481 ggaacaggcc aaatcgttat tgtcagatta caaagtaacg gatacctgcc agaacctggg
    21541 atattccggc gacctggtac ggggatgtga agcgacgttc ctttctcaac tggcaagcgt
    21601 ggaatacggt aacaagctgg agagcaagct tcgtcaggac aaccagacga tgaaagacct
    21661 tattgatcaa gtcaaaaatg cgaaggatac gaaggccacg caggatgcaa caaacgctgt
    21721 tgcacttgaa caactgaagt tcgagaagct caaatttcag tatcaaatgt atcgcgataa
    21781 gcagcgagat cttgcagaat ataaagagaa gatggctcag gcagctttca gaaaacagca
    21841 acgtgaagcc gtgccacctt cttacagaaa agcttatatg gcaatgaaat catatgagga
    21901 tgattaaatg aaaagtatcg ctactgctgg ccttgtcatt atatctgttt ttagtctttc
    21961 tggatgtttt gaagaaacaa aatcggttga ttggtggctt gcacatccga aggagacata
    22021 caaaaaattt gaagagtgtc agaagtctgg tagcgattct gataattgta agaacgttaa
    22081 gcgagcacat ttgtcttttg aacggagaaa ggcggttggt ttaccaataa attgagggtg
    22141 atgtatgggg attgtcactg gtataccgga tggtactgat ttatcaggta atgtaacctc
    22201 tcccgcaaat gcgggaggtg tttctggatt tgattctgat ttttttcaga caactcatga
    22261 agttatattt aatattctca acaagagtat atctggaaaa ttaagtgaat attcagatgt
    22321 ggcttatact cttggtaaat atggagtttc tctgtatgtt ttatggtatg cttttactgt
    22381 attagcaagg aaacaacaga cacctgtacc tgattttatc tggaatatct gtaggtttta
    22441 cataatattg ctttttgtta agaatacagg gggaatactt acatcagcaa cagatgctat
    22501 tgatggattg aaaaatacat tggcaggggg agatccgtgg gtatggatgg atcagttatg
    22561 ggtgaaggtt atacaagttg caactcttat ttttgataaa gatacatcta ctgtgcctgt
    22621 tgccggtggg attggtgctt tattaactta tgtcggtggt gttttggcat tattgctttg
    22681 ttctatagta tttgcatctg ctgaattaac attactatta ctttctgtca ctgcgccaat
    22741 atttatcatg tgtctgatgt ttggtttact tcggcaaatg tttaatagct ggctacagct
    22801 taattttagc tcgttactgg tttttctatt tgcagcatta gcactaagag ctgggacatg
    22861 gcaattaaac atggcattaa gtacgtctat tgctacagca tcagaaaaca atcttcttca
    22921 aacgggagta acttcattag ctgctggcat tttcatggcc tggattatct ggcaggcgaa
    22981 aagttatgct tcacagattg caggtgtggg tgttgaaggt gccatgcagg gcgcagccgc
    23041 tatggggatt ggtgctggcg ttttcggtgc atcccgtatg gcgcgtggcg cacttggtat
    23101 gggcagaaat gccggtattg gtgcatggaa aggtctgcgt cgtcaggaag acgggtttgg
    23161 tcagtctccg ggaataacgg gtaagaccgc taacctggcc ggacagggcg ttaatattgg
    23221 tgccaaaaag cttcgtcagg cagctattga gagagcaaag aaaatgtatg gtgggtaatt
    23281 taattatgag cgccttttaa tgttcttttc ataattgtca ttgtcgggcc tggcgcgctc
    23341 tattatcttt tctgttcaca ctgagtcttc tgtaaatatt aatctgttat acaggtgttc
    23401 atattatgaa actacttatt gtggctttcg tgacgctttg tctcgctggt tgtcaggcgt
    23461 cacataaact accgcccgtt tccgggaaaa gcgaacctgt taattctgct gaggtaatgc
    23521 aaaatggaat ttaaacttcc cggatttaaa aataaaaaag acgttactga ctcatcagtt
    23581 tcatttgaag aaaaaaacat tgcactacag gagagaatga atcgtattta taaattcggt
    23641 ggtatcggag gcatgttaat tggtgggctg tctttacttg cattaaatgc agcattacca
    23701 ctgaaaacaa cagttgttga tgcctacctt atcgataagg ttacaggtgt ggctgaacgt
    23761 ctgacttctg ttaaaaaaga aaatctttct gaaaacgaag ccattgcccg atattttatc
    23821 acccagtata taaaacatcg tgaaggttat aattttttca gtctccagca tgattatgat
    23881 tatgtaatgg cttacagcgc ggagaatgtc gcggcagatt ataacgcatt atttaacagt
    23941 gaacaggcac caaaacttgt ttataacaaa gcagaaaaaa cggcaatggt tcaggataat
    24001 ccatctgtca taatttcacc ttcgtcacgg gcagatgata aagatatcgg tgcgtatatt
    24061 cgttttcgtc tgaccatcag ggatgttgct accggacaaa cccgccagga gttctggaat
    24121 gttcgcctga cttatcgtat cgaaccgcag gttgaaatgg tgtcagggga acgtaataac
    24181 aatcctctta aattcgttgt aacaagctac gttcgcgata aagaagccag aggttaataa
    24241 tatgaaaatg aataaaggag cgttaattat ggcgcttctg atggcggcgc acgtctgtca
    24301 tgcagctgtt cttccttcag gcagtcgctt tgacccacgc aatcagatag tcagttataa
    24361 ccccaataat accaccataa ttaacagtgc cgttggatac accaccacac tggtatttga
    24421 tgaagatgaa acagttatca gtgccagaac tggttttccg cagggatggg cggttaataa
    24481 agaagataac ctggtatacc tggaagttcg tcctgttaaa cagactgttc agaaaaataa
    24541 tatggatgaa aacggtaata cctcttctga atccgtcagt gttgctcttg acccggaaaa
    24601 tgagcttgaa cgctggcgaa cgaatttgtt tgttcgcacc acgaagcgta attacagcat
    24661 ggagctgaac gcccggacgt tccggcagcc ggagaaaatt gcgtttgtgg tgaattacca
    24721 gtatccgcag gaacgccgga aggaacaggc cgaaattgag aagaaacgca cagaggctct
    24781 tgccagacgc caggaggagc aggcaatcaa ccgttccctg gaaaatgcga aatcgccccg
    24841 taactggcag tactggaagc gggttgctga aggcagccag gatatcagcc ctgattatgc
    24901 atatgacgat ggccgttata cctggttcgg cttcagtccg ttaaagaaaa ttcccagcgt
    24961 ctttgtgatg aacggtatgc aggagactct taccaatcct gtgattaaac agagcgggag
    25021 ttttacggct gttggcgtac cggttgataa gcgttttgtt ttacgtcttg gtgagcaggt
    25081 ggtggggatt gagaaccagg gcttcggaaa agtacgttta ccagccggag atacggtatc
    25141 cccggatgtt aagaaagagg tgatccagtg actgaacagg aaaataaaat cccgactgca
    25201 accgaaattg aacagcagct acgggaacgc agacagaaag aactggaaca ggccgggaag
    25261 actccggaag aagagcctgg caagccagca ttgcagcttg gtattgaaaa acttaaaaag
    25321 tcacgtaaag ggatgattat cctcgtcgtg ggttttcttc tgcttgctgc cggtgtttct
    25381 gtttattata tcccgtccat tatccgttct gtgtcgtcag gggatgagaa acccgcaagt
    25441 cagccgcttg caaccggaac ggctaaacgt cagaccggac tgagcgaaga tatcgatcct
    25501 tttaataccg cacagaaaaa aacagagaaa ccagaggaag aaaaagtcat ttcttctgaa
    25561 aagactgaac cgccggaaaa taaacagcag agcttcagcc gtgcacttga cgtttctctt
    25621 gatggcagcc agacaggaaa cagcagcagt tcagcgggaa cgtcagtttc acatactgcg
    25681 gccagtgagc cagaaagcga taaaaaggat gaagcaaaag caaccgcaca gactacagaa
    25741 tctgcgccac tggcgaaaat aacgaaactt ccatatgacc caaatttgtt tatcccggaa
    25801 gggacatcaa ttccctgttc actggacagg cgttttgttt ctgacctggc ggggaaactg
    25861 gaatgtacgg tcaacagcga tatatacagc gccagcggta atgtaaaact tatcgaaaga
    25921 ggaaccgccg caaaactgat gtataaggcc gggtctttaa atcatggaca agggcgtgtg
    25981 tttgttatgg cttacaagct acgtacccgc agtaagcctt ttattgatat tcccctggtt
    26041 gactcacagg cggctggcgc gttaggcgaa gccggtgctt ctgggtggat tgacactcat
    26101 ttcagtgaac gttttcttgg tgcaatgatg gtcgggatga taccggattt aagtcaggcc
    26161 gccagtggta ttgcacagaa caacagggac agccagaccg actatacggc aaacagtcgc
    26221 caggcttttg ctgatatagc acgcgaagca ttttctaata gtgtgaatat tccgccaacg
    26281 ctttataaaa atcagggcga aattattact ctgattgtcg gtcaggatct ggatttttca
    26341 ggcatttata aactgaaaat gaaagggggt taatgtgaat aacgaaaaca gacatctgat
    26401 ttatgatgtg gtcaacgatt atttttatca ctggctgaat gagattgagg gtgtcacgga
    26461 aattgctgtt aaccgaccag gagaaatatt tataaaggtc aggggaaagt ggcaatggta
    26521 tgaacaaaag atgagttaca gtgattgtct ttcttttgca tccacactgg ccgattttca
    26581 tgacggcggt tctgtgactc ctgaatatcc cctgcgctct gccacgcttc cgggtggaga
    26641 acgtgttcag gttgtgatcc caccggcaac tgaaaaagac actgtttcta taacaatccg
    26701 taagccgtca ggtattttta tcagtcatga caaatttata aaacagggat tttattcacg
    26761 cgtcagtggt ttaagtggtg actcggttat ggaagataat atttctgctt taatcacttc
    26821 cggatatttt gatcgggtta tacctgaatc actgcgtcag gggaaaacga tagttttctg
    26881 tggagggacg ggttcaggta aaactacctt tgcaaatgcc tgtctggaat atataccgca
    26941 tcatctgcgg tgtatttcta ttgaagatac tgatgaggca aaattcagat tccataaaaa
    27001 ccatgtaaaa ctttactatc cggcagaggg tgagagtaag gttattacct cagcgagtct
    27061 tctgcgttcc tgttttcgta tgaatccgga caggattctg atgacagaaa tcaggggggc
    27121 tgaggcatgg gattttctga aagcatcgag ttcaggccat gcaggaaaca ttaccaccgt
    27181 tcacgaaagt agtcctgaat atgctgtgct tgggattgtt cagcgatgtt atatgaatcc
    27241 tgaatgtcag aatctaccat tcaatgtcat tttaagacgt gtactgagta atattgatat
    27301 tatcatgagt attaaatacc ttgatgatga agattttcgt ttcgcttccg gtatttatta
    27361 caaacaactt cattttgatg actatttcag aaaactgaag gagtgattat gtctttaaaa
    27421 ctccccgata aaggccagtg ggtttttatc ggtctggtta tgtgtctcgt gacatattat
    27481 actggttctg ttgctgttta cttcctgaac ggaaaaacgc cgctttatat atggaaaaat
    27541 tttgattcca tgctcctgtg gcgaataata acagagagta atatacggac agatatcagg
    27601 ttaaccgcta tcccctctct tttatcaggt atggtttcgt ctctcatcgt gcctgttttt
    27661 attatctggc aactgaataa aacggctgtt gctctttatg gtgacgcgaa gtttgccagt
    27721 gataatgatt taaggaaatc gaaacttctg aaatgggaga aagaaaacga tactgatatt
    27781 ctcgtcggag catataaagg taaatacctg tggtataccg caccagattt tgtatcactt
    27841 ggcgcaggaa cccgcgcagg taaaggtgcc gccattggta tccctaatct tctggtcaga
    27901 aaacactctc tgattgcgtt agatccaaaa caggaattgt ggaaaatcac cagtaaggtg
    27961 cgtgaaatac tgctgggtaa taaagtttat ctgctcgacc ctttcaacag taaaacacac
    28021 cagtttaatc cccttttcta tattgattta aaagcggaga gtggggctaa ggatctgctt
    28081 aaactgattg aaattctgtt tccgtcttat ggcatgacag gggcagaagc gcactttaat
    28141 aatcttgcgg gtcaatactg gacaggactg gctaagttgc ttcatttctt tattaactat
    28201 gagccgtcct ggcttaatga gttcgggctt aaacccgttt tctcaatcgg ttctgtcgtc
    28261 gacttgtaca gcaatattga ccgggaactg atactcagta agcgggaaga actggaggga
    28321 acaaacgggc ttgatgaaaa cgcgttgtat catttgcgcg atgccctgac caaaatcagg
    28381 gaatatcacg aaacggaaga tgaacagcgt tcaagcattg atggttcttt ccgtaagaaa
    28441 atgagcctgt tttatctccc aaccgttcgt aaatgtactg atggtaatga tttcgatctc
    28501 cgtcagttgc gacgggaaga tatcactgtt tatgtcggtg ttaatgcgga agatatatca
    28561 ctggcttacg attttctgaa cctgtttttc aacttcgttg ttgaagtgac attgcgtgaa
    28621 aatcctgatt ttgatcccac cctgaaacat gactgcctga tgtttcttga tgagttccct
    28681 tcgattggtt atatgccaat tattaaaaag ggatcagggt atattgcagg tttcaaactt
    28741 aaactgctga caatttatca gaatatcagt cagctaaatg aaatctatgg tattgaggga
    28801 gccaaaacgc tgatgagtgc tcatccctgc cgtattatct atgctgtcag cgaagaggat
    28861 gatgccgcga agatatcaga aaaacttggg tatattacca ctacatcaaa gagcacaagc
    28921 aagaaccggg gacgatcaac ttcacagggc gaatcagaaa gtgaagcccg aagagcactg
    28981 gtgcttccac aggaactggg aacgctggac tttaaagaag agtttatcat cctgaagggg
    29041 gagaaccctg ttaaagcaga aaaggcactt tattaccttg atccgtattt tatggacagg
    29101 ttaatgaagg tcagtcctaa actggcatca ttgacgatga aactaaataa gacgaaaaaa
    29161 atatttggtg tgaaagggct taaatatccg tcaaaagaaa aaatgctctc cgtaggagag
    29221 ctggagtctg aggttttgct atgaaaaaaa tacttatgat cagtattctt gtcctcacag
    29281 cctgttcctc tccacctgaa ccgccacagg ttgaatggga aaaaaggcct gaagttatga
    29341 atacacaaat aatgaactgg aaaccaacat ccggtgttat taaatcaaat aatataaatt
    29401 cctcatggag taaggtgttg cctgatttta aaccagaaaa ccatctttac gatgattctg
    29461 ttttttatgc cgttgcccat tctgaaaaaa tagttgtaag gacatcttct tttgatagtt
    29521 actggtcagc gaaagactgg ctaagaaaaa atggtgcaac aggtgttatt gaatatcagc
    29581 cactaaaaag atggttgaat aatgactatg ttgaaattta tctgtcaaga ataaatattc
    29641 agaggttact atgaaaagga tatgcaaagg tctaatcgta atttttactg tgtcctgttt
    29701 tatagcgcca acgtatgccg ctgatccctg taaatccgtt ttttgtcttt atggtaaagc
    29761 tgtcggtagt agtggaggga gtgagtgcag cagtgcagaa aaggattttt ttaaaaatgt
    29821 tgaaaagaaa aaggggaaaa tccgctgggg taaaacattt gatctccgta agaatttttt
    29881 aaatcagtgt tcaacggcag acccggctgc aatctcactg atcatgagta agtttggtcg
    29941 cgtcagaggt tgattaaaac ctgatacccg cgccaacggg tatcagggga ctgcaagtat
    30001 cacataccgc gccaacgaaa tgtgatgatt agttacaatc actgaggtta gaaatgaaag
    30061 taaccaaatc tattatatct attttattgt gcttatctgc tggtagtgca taagtggatg
    30121 taataaaaaa tgctcgtacc tttttgaaag gaacccttga tctgagcgtg caggaagcag
    30181 atgaaaggga agaactttat aagaagaacg gggcgcaacc tgactattta agttacctgg
    30241 aggattaaca catgcaatat gcattatttg atgggatgga acgaaagttt ttgctggatg
    30301 ctcttgaatt tggtgttctg aaggactgga aagaaaatcc ggtaaaagaa cttcctgata
    30361 ttgatgaatc tgttcacccc ttccatgtct gttatggtgg atatttatta aaccctgatg
    30421 tttcagattt agatattagc agaaaaataa aagaccagac aggattctgg ctggcagcta
    30481 ttgatgatac ccgtatggat tgtcattcaa tagcttatta tgatattcac accctccctt
    30541 taatttcgtg tggtcatcag aagatagttc cttttgcagc gttaataaaa gctgatgaat
    30601 gcatcatttc aaaaattgct tcgtattctg gttttgccgt aacagccttt ttgagaatta
    30661 aagaccagaa tatcgcaacg aatatactta accgtgaggg aatttttgcc tttaatggct
    30721 gtgagcacag attcagacaa cctgtaagtg aagataactg gcaacaggca gtgtcagaag
    30781 aacgcgctat ccgttgtgcc aaaagattaa ttcaatgtaa aggataacaa aatgagactt
    30841 tttatcgctg aaaaacccgc agtagcaaat gatattgtta aggcacttgg tggcaatttt
    30901 acccgccatg atggctggtt tgaaagtgat aacgccattg tgactaactg ttttggtcat
    30961 attatcgaat cacaaccgcc ggaaaactat aatcctgaat acaaagcctg gaaggttgaa
    31021 acgcttcctt tacgtcttta tcccgtgaag tatcagcctg ttgaaagtgc agcaaaacag
    31081 gttaaaacga ttctcgaact tatcagacgt ggagacgtga ctgaaattgt tcacgctggc
    31141 gatcctgatg atgagggaca gctacttgtt gatgaagtcc tggaatatgc aggaaacaca
    31201 aaacccgtaa agcgcgttct gataaacgac aacacgcttc cggcagtgaa aaaggcactg
    31261 gcaaatctta aagataatcg tgatttcaaa gggctttacc ttaaggcgct ggcgcgttca
    31321 gttgccgatg ccgtctatgg attctccatg acgcgtgctt acaccattcc tgcaaaagcc
    31381 agaggatatc agggcgttct gtctgtcggg cgcgtccaga cacccgttct tggcctgatt
    31441 gtgaatcgta cccgtgctaa ccagaaccat aaatccagtt tttactacac catgaccgga
    31501 gtttttcagc gtggtgctga tgttctcagg gcgaactgga aaccaggtga atttgctccg
    31561 ctgaccgacc gtaaattact tgataaggcg tgggcagacg gaacggcagc atcccttgca
    31621 ggaaaaccgg ctacagttga agcagcagca actgatgata aaaaaacggc tgcgccgttg
    31681 ccgtttaacc tggtcagact ccagcaatac atgaacaaga agtttaaaat gacggcacaa
    31741 aaaacgctgg atattacgca acaactacgt gaaaaatata aagcaattac ttataaccgc
    31801 tcagattgct catatctttc tgatgaacaa ttcagtgaag cgccgcaggt tatcgatgcc
    31861 ctgaaatcag tctttcctca gtcgctggat attgattctt cacgtaaaag caaagcgttt
    31921 aacagtgcaa agtgactgcg catactgcga taatcccgac ctccagtgtg cctgatgtta
    31981 acgcactcag caccgacgag cgcaatgtta cctggcgatc gcacaacact atcttgttca
    32041 gttcatgcct gaaaaagcat accaggaagt atcggttgcc attcagtgtg gtgatgagtc
    32101 gttctatgcc cgtgccagaa aaacaactga cagcggattt gaggcgtttc ttggcgcgga
    32161 aatcacagac gaaggtgaat cagaagataa tgatgattcc gcttttgaac tgctctgtaa
    32221 aattcgcaca ggagaaacac tgacgacaaa agaagttgtt gttaatgaga agaaaacaac
    32281 accgctgccg ttattcaccg aagcctcctt gcttgctgcg cttgttcgtg tcgcggattt
    32341 tgtcactgat ccaacgatta aaaaattact gaaagataag gataaagaca aaaaatgaac
    32401 atggcggtat tggtacgcca gctacccgtg cagccattct ggaaacgctg aagaagagaa
    32461 actatatcac gctggaaaaa gggaaactta ttccgactga taccggatat gcgcttattg
    32521 atgccctgcc aggtatagcg gttaatcctg atatgacagc attatgtctg aaaagcagac
    32581 tgccattgaa aatggcgacc tgacggttga acagtttatt aatgagctgt acggtgaatt
    32641 gacaggcatg atttctgatg ttgacctggg caagatgaag attgaacccg ctgcgccagc
    32701 agggcagttt caacgcctgg actctccctg cccttccttg tggtaaacat attgttatca
    32761 ggccgaaagg ttatttctgt accggatgtg aatttaaaat ctggagtgag ttttctggta
    32821 agaaaatcac ccaggcacag gccgaaaaac tggttaaatc agggaaaccc gatttgatta
    32881 agggatttaa aagaaaagtg gtggaacgta tgatacagtt cttgtccttg aggataagaa
    32941 aacag
//
