LOCUS       NZ_CP028998            10077 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain AR_0079 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP028998
VERSION     NZ_CP028998.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014920
            Assembly: GCF_003073335.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Conlan,S., Thomas,P.J., Mullikin,J., Frank,K.M. and Segre,J.A.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae AR_0079
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2018) NIH Intramural Sequencing Center, 5625
            Fishers Lane, Rockville, MD 20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.6
            Genome Coverage                   :: 98x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:39:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,243
            CDSs (total)                      :: 6,114
            Genes (coding)                    :: 5,981
            CDSs (with protein)               :: 5,981
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 64 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 15 of 133
            Pseudo Genes (multiple problems)  :: 16 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028998.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AR_0079"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
     gene            complement(436..585)
                     /locus_tag="AM458_RS30950"
                     /old_locus_tag="AM458_30940"
     CDS             complement(436..585)
                     /locus_tag="AM458_RS30950"
                     /old_locus_tag="AM458_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(670..927)
                     /locus_tag="AM458_RS30955"
                     /old_locus_tag="AM458_30945"
     CDS             complement(670..927)
                     /locus_tag="AM458_RS30955"
                     /old_locus_tag="AM458_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(937..2622)
                     /locus_tag="AM458_RS30960"
                     /old_locus_tag="AM458_30950"
     CDS             complement(937..2622)
                     /locus_tag="AM458_RS30960"
                     /old_locus_tag="AM458_30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2949..3194
                     /locus_tag="AM458_RS30965"
                     /old_locus_tag="AM458_30955"
     CDS             2949..3194
                     /locus_tag="AM458_RS30965"
                     /old_locus_tag="AM458_30955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3468..3839
                     /gene="tolA"
                     /locus_tag="AM458_RS30970"
                     /old_locus_tag="AM458_30960"
     CDS             3468..3839
                     /gene="tolA"
                     /locus_tag="AM458_RS30970"
                     /old_locus_tag="AM458_30960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3836..4054
                     /locus_tag="AM458_RS30975"
                     /old_locus_tag="AM458_30965"
     CDS             3836..4054
                     /locus_tag="AM458_RS30975"
                     /old_locus_tag="AM458_30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            4396..4674
                     /locus_tag="AM458_RS30980"
                     /old_locus_tag="AM458_30970"
     CDS             4396..4674
                     /locus_tag="AM458_RS30980"
                     /old_locus_tag="AM458_30970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4662..4976
                     /locus_tag="AM458_RS30985"
                     /old_locus_tag="AM458_30975"
     CDS             4662..4976
                     /locus_tag="AM458_RS30985"
                     /old_locus_tag="AM458_30975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5140..5568
                     /locus_tag="AM458_RS30990"
                     /old_locus_tag="AM458_30980"
     CDS             5140..5568
                     /locus_tag="AM458_RS30990"
                     /old_locus_tag="AM458_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5594..5773
                     /locus_tag="AM458_RS30995"
                     /old_locus_tag="AM458_30985"
     CDS             5594..5773
                     /locus_tag="AM458_RS30995"
                     /old_locus_tag="AM458_30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5801..6331)
                     /locus_tag="AM458_RS31000"
                     /old_locus_tag="AM458_30990"
     CDS             complement(5801..6331)
                     /locus_tag="AM458_RS31000"
                     /old_locus_tag="AM458_30990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6338..7069)
                     /gene="mobC"
                     /locus_tag="AM458_RS31005"
                     /old_locus_tag="AM458_30995"
     CDS             complement(6338..7069)
                     /gene="mobC"
                     /locus_tag="AM458_RS31005"
                     /old_locus_tag="AM458_30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7069..9030)
                     /locus_tag="AM458_RS31010"
                     /old_locus_tag="AM458_31000"
     CDS             complement(7069..9030)
                     /locus_tag="AM458_RS31010"
                     /old_locus_tag="AM458_31000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_065799589.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7622
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4..333
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.494"
                     /coverage="99.0"
                     /evalue="4.0100000000000005e-164"
     Typing          9832..10077
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.581"
                     /coverage="99.0"
                     /evalue="9.26e-116"
     Typing          complement(6342..7069)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 tgcgggaagt gtggcgcttt ctcatagctc acacactggt atctcggccc ggtgtaggtc
       61 gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta
      121 tccggtaact gttcacttga gtccaacccg ggaaagcacg caaaacgcca ctggcagcag
      181 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgaggt tgctcttgaa
      241 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
      301 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
      361 aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag
      421 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc
      481 caccgttccg ccctgaacat cccggatata atttgcctga catgcggcca gaagaaagcc
      541 agcaacaatc aatataaaaa gaaaaatagt ctttaccttt ttcatatact ttccctgtat
      601 ttaaagactc cggcattcat ccctgaacac cggaagtcag tatttcaaat catccttgat
      661 aattttttgt taccagttac cgtcccgata atcaaaagct atataacaat tatccacatc
      721 aggttttatt tgatttttaa aaaatggctg tagtaaagga acccattctt tcttcaaatc
      781 aaactcacca ttattaatat tatcctttaa aggcattccc aagctttcaa tgacagaacc
      841 atcgtcacca aagtctttag aatactcttc ccctttaaac tcttcagttt tcttatcaaa
      901 ccatgtcaaa cgtaatttaa gccccatatt tacctctcaa agatattttt taatgttccg
      961 ttttggatct ggccctttaa cctgcttacc cgttttggga tcaaatgccc cgaggtgttc
     1021 gccatcactg gcacgatacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     1081 acgtttttta tcgccatacc atcgggcccg cttaccacca ccaccttgtt taggtgtttt
     1141 aggatcacct cgctttaact ctccaagtcc ctttatgtct tctgttttag gtactggatg
     1201 ataatcatgg ccgtagtctt tagtcccctt gcgaggcttg ttcttttcct catttaactt
     1261 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggtaag
     1321 tgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct gagccgcttt
     1381 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     1441 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     1501 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggagcg ccttctctgt
     1561 cgatgcaatg cggctgttta aggtcgcaat atttttatct tcggcatcca gctcagcttt
     1621 cgctttatca tagtctcttt tcagcccctc ttccggatga gcggcgtccc atgcctgctg
     1681 gcgacgcttt tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa catctgtcac
     1741 cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga
     1801 gttaccaccg gctgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt
     1861 tggcggggct ttcgctgtcg gaacaccttt cggtacgctc acctgcagag acgggagacc
     1921 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacagggac
     1981 actcattggc cgacctgcga caaccgcaat atgctgccgc tcatccttca ccacatccac
     2041 aacccgttgt ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg ccggagtctc
     2101 cgtcaccgca tcggccggta atgacgtcat aatttttgac atcattttcg gatcatcttt
     2161 tgcaatctca gaaggcagca caccgtagat cgcaatcccc cacagaccaa acttaaacgg
     2221 ccccttcagg gcagccagca catcagcaac ggcggccgac aacgcatcgc cggaaactga
     2281 taaagccagt ccttcagccc ccggagtagc cagggcaggc agaccgaagg ccatcgcagt
     2341 ggccgaagac tgtccgccac cagagctacc gctatgattg ccgctgttac caccattacc
     2401 accgggttta ctcccgccgt taccaaactc ccccggatta tagttatgaa tatctccatt
     2461 cggcgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     2521 agggccacct ttaccagacg taccgttcac attcccactg gcctgaccac cattatgtcc
     2581 cagacctgaa ttacccggac ctcgaccgtc tccaccactc atatcgtttt cctctttgac
     2641 atttaaaaca ataaattaaa gccaatatac tgtacatata cacagtataa ttattacctt
     2701 actcctcccg ttttcaggtg tcaatatatg agattagttc tcatcttcag gcacaaaaaa
     2761 gcgcccggag ccacctgtaa gcagatcgcg tcttttcccg aaaatttctc acggcaacaa
     2821 attttattat ttgagatgta catcacactt acatttcagg tggtatagac acatctgaaa
     2881 gctaactggt atgaggggag gacccgcggt taaactgttc aggaaacctg agacgcccgg
     2941 aggccaccat gacacacctg aaactgactc ccgctgaaga aaagcgccta gcggacgcct
     3001 ttgtcgctca ggcactggcc atgggcctga aagggaacag aacgctgcca aaggcggtaa
     3061 aacagcaggt gcgggaaagg gtgcttcgcg ccattcagga cgaacgtgaa gccaggcggg
     3121 cgcaggaaat gcgtcgggag aaatacgagg cggcggaaac caccttcacc tggcagccgc
     3181 tgcgccgccg gtgaggggta tttaccataa aacccgttat cagcaccgcc cgagaagccc
     3241 tcagagcgtt tccggcggct acgggataaa acaggcaaca aatcacccgg aaaaccgccg
     3301 gaaacgcgcc cggtaaattg taacccgctg catgcctatg cacgcaactg catggcgggc
     3361 cgtttggtgt aaaaacgata cgataagccg tgtttatgtc aatgaacgtg gccgtaaaga
     3421 gtggcaaata tacatgtaat attttttact gacaggtcat caacccgatg cgtttaatac
     3481 cctttatcct gctggcattg tgcagcccct tactcctgac ggcctgtaag cattccacca
     3541 cccagccgcc gaaaatttat actctggata agtcatctaa aatcagtagc tacgctgcgg
     3601 ccatcaagac cgctgttgaa atgcagatgt ttgaaccaga ggcctatgcg ggaaaaatgt
     3661 gcaccgtgcg gatatccata caacgtgatg gcactctgaa cagcgccatt gctgaaggag
     3721 gagatccgaa actctgtaaa gcagcaatat cagccgttac ccacgccaaa attccgccgg
     3781 caccggatga aaatacctgg caaatattca aaaacgcccc tctggatttc agaccatgaa
     3841 agcttttact ctgacagtgc tggcggccat gttcctcact ccggtacatg cgataccact
     3901 aaaacccgta accacactgc agccaaccta cccggtacag gccgcagcaa aacgcatcga
     3961 aggtcaggtg accgccacgt ttgatattct ggacgatgga cgagtggaca acgtgtcgct
     4021 taccggctgt cacttttttt tcgggaaacg ctgacggcaa tgaggcactg gctatatccc
     4081 gcagggcacc cggcaatgcg acaggttata aattttgaat tcagtctgaa aaatgacggc
     4141 ttacaggatc cgcgaccagc caccaccaaa aagttcaatg atgatggttg ccgccagtaa
     4201 caggtccttt aacccgcagg ctgcaatagt cctggatgat gagtgagcct gtagcagccc
     4261 tgattagtgg aagcgaagct gaaaaatggg gaagcgaagc tgaaaaatgg gtagcaaaag
     4321 ccgtatttct ggcaaatagc gccgccacgg attaaagtag tcatatgtat tacacatgac
     4381 tacaaagggg cttttatgtc atcagcaaca tccatcaggc tcgatgaaga acttaaggac
     4441 cggctgaaaa cgctggccga tgaccggcac cgctcagcgc acgcgctaat gctggaggca
     4501 atcaccgaat acatcgaccg ggaagagaag cgcagccagt acctgcggga cggacaagcc
     4561 gcatggcagc actatcagga aacgggtctg caccttactg cggaagaagc agaagcctgg
     4621 ataagtacct ggggaactga gaacgagcag gatgctccgc catgccacag gtaacgattt
     4681 ccgcgctggc acagcgagat ttacaacgcc tccaggactt tctcaaaacc aaaaatcggc
     4741 tggcagccag aaaagccggt gaggtgatcg tccgggctat ccagcaactg aaaacattgc
     4801 cagacattgg ccgcccggtg ccatttctgc cgctggaata tcaggaactg gtgatcgggt
     4861 ttggcgacag tggctatgtg atgctctacc gccacgaccg ggaaatggac cggattgtga
     4921 tcgtcaccgt caggcatcag aaagaatccg ggtatccggg ggcagacagc ctctaataac
     4981 cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt ttgacaagca cagcgcgtca
     5041 gaaaaccaca tcctgaacgg aaaaacgggt aaaattcagc gatacattta ccgtgagaaa
     5101 ttacacatct ttcacacccg ataaaatcag gggtatacag tggcgaaaac attgctcaac
     5161 ctttcacagg cagcacaagc cgcgggcatt accagaagaa cgctatacaa tcatgtaaaa
     5221 cagggaaagg ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga tgtgtcagaa
     5281 ctgatccgtg tatacggaaa tgtgaatatc cctgagaaac aaattcccgg catttcacac
     5341 cgtgaaaata cacaaaaaaa tttcccacag gaacagcttc tggcaatgca aaaggaactg
     5401 gccgatctcc ggcaggctgt caccctgatg ctggaagata aaacatcccg agaagaagaa
     5461 cgccgccagc acgatgatga gcgccggaaa ctccaggccg aagtggacag attgacaaca
     5521 gaactgacac agaagaaaaa acgcttctgg tccggctggt ttagctgaga acttaccgaa
     5581 gggaatggag aacatgacga agcaggaaaa aaccgccctc aatatggcta aattcattaa
     5641 ggaccagtcg ctgctgttgc tcgagaaact gaacgagctg gatatggata ccccggccga
     5701 catgtgcgag cggctgcacg aggatgcgga aacactctgg caggtgatgc aggataagct
     5761 cggggaagaa taacagcacc ggcatggccg gtgctggcgt ttattttttg ccgaacggcc
     5821 acctccggcg ggcgggttta tcctccggca gttctggcac cggaacgtta aaatagcggc
     5881 tgttatattc ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtacc
     5941 gctcactcac gacgtcaatc atcgccccga tctgctccgg ggtgtatttc ccggcgtcca
     6001 gtacctgcac gccgttctcg atggcgaact gctcaacctt gcgataaaga taatcacaca
     6061 gcttcattcc ggccggtttc tcacgttccc acgccggatt tttgccggtg aagtaatacg
     6121 tggctttgtc atcgctgttc agccgttcgt gatacatgcc gataaacttc atcattgcca
     6181 gatgctgggc ctgatcttca aacctgaata catatgcacc ggtctgccag gccatgaccg
     6241 ccatgtcttc cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca
     6301 cgtcctcaat aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa
     6361 agcatgcgaa cagcctctcc cggtccagct ccgccgtttt gcgggcctcg ccggtctgcg
     6421 ggttcacgat ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt
     6481 cagggcagat gtaccagatt tcatcgatgc cgttcgcctt acggttgaac agatacccgg
     6541 acaccacttc ctgataccgg cgacggctct tgacggtacg ttccacctca aacgccaccg
     6601 ttctgccgtc cagctccgcc agcgcgtccg gcaccttcat ccctttcagg gcaatacggt
     6661 gcaggtaccg ccagccggtg gccccccggg cctccatggc gaggcgcagg gactgcacgg
     6721 ccagccggtg cggcagctgc gccgccgaca cccgcgacgg ctgaaacgga ataatgtccg
     6781 tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc
     6841 tgacctgacg gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccaggc
     6901 tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgttgc gcaaccagct
     6961 ccgccgtgga acacagttct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt
     7021 tccgtgcctg ccgcttcgcg tggctgacgt tgtatcgctc cagtgccatc agtacagccc
     7081 cgcaaaatca tcatcacccg gccgctgccg cccggcctcc gactggtact ccgccagcgg
     7141 gtccgttttc gccagcacag ggaaggtttt cagcggaatt tcccggcgat caacacgcag
     7201 cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg
     7261 cagatgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc
     7321 cccgctgata tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac
     7381 ccggatctcc ccggtctgtc cggccgccca ctgagcggac tcctggctgg ccgcccggta
     7441 gaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc
     7501 ctgcccgcac tggccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg
     7561 gctgcgcagc gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc
     7621 gagcatgatg ctcgcgtgcg gccaccaccg gaactcgtcc cgcgcaatga tgatttgcgc
     7681 gcagcgggca aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat
     7741 gacgtacagg cagccgccgc cgttgattat ccccgccacg tcgatgccgc tgtccgtctg
     7801 aaggacgctc aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt
     7861 cagggcctcc ggcagtgcgt acgccgccgc cagcacctgc tgaatatttg ccccctgcgg
     7921 aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac
     7981 gtcgccggtc tcactcaggt taaatccggc aaccagaaga ttcttcaggg cgtactggtc
     8041 gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa
     8101 gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg tcccctttcg ggtcgaagta
     8161 caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct
     8221 cttgccgctc ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg
     8281 gaccagcacc ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc
     8341 ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg
     8401 gcggacgtcg gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt
     8461 cgcgttcacc gcctcgtcag cggccgcaaa atgcccgtca agcagcagga acgccagcag
     8521 ccgcgccagg aggacgcctg cgacaagacc tccggcacac caggcaatcc cgaacaggtt
     8581 agccggctgc agcaggtaat aatctgcgtt cgcgtagagg tccggacgca ggacggtttc
     8641 tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc
     8701 gccggtcagc cagtatgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg
     8761 cgaatccagc gaggccagca ccgaaaatgg cgcagtccgg ttattatcgc gcttatagcg
     8821 atcggcatac tcccgccgct ccttatgact cagccccacg aatcgcatcc gcccggccca
     8881 gagttctgcc agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata
     8941 ctcccggtac agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca
     9001 ctgccaggca agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta
     9061 ccggcgtgtt gtgcagcccc tgctgcacaa gccgcggaca tgccccaagg catggcggcg
     9121 gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc
     9181 ccatgcggaa aaggcgcagc cccggaggaa caacgagcac gccccagcgt gtgagaggaa
     9241 aaggcggcac cccagtgcgg tcttttccgg tgttccggcc cccgtcagag ttcgccatag
     9301 cgagcggctg accacccgca aaatcaggag cagctttgct gcttcgtcag gtgcgtccag
     9361 caccgtggat tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc
     9421 gtgaccggcg tccagccgga cagagccaga aagaggggga aagacagtgc gtcggtggcg
     9481 tccagccagc gtaagcaggg gatttccccc tgaagtcagt cacgccccag cggggatgac
     9541 gtgcatttct ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa atattcagca
     9601 gaaaaccgac agacttcacc tatctgaata atttatcggc agggaagaga aaggcggtaa
     9661 cacggagcaa tatgtcacat ccacgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt
     9721 gcgcctcctc gctcactcgg tcgctacgcg ccggccgtga gactgcggcg ggcgctgcga
     9781 actctcgcaa agttacccac agattccgtg gataagcagg gaacgagcat gtgaggcaaa
     9841 acggcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     9901 taaacagacg ctttttcggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     9961 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggtagg tcgctccctc
    10021 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgtccgccgt tctccct
//
