LOCUS       NZ_CP028857             4096 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 3214 plasmid p7_3214, complete
            sequence.
ACCESSION   NZ_CP028857
VERSION     NZ_CP028857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08915824
            Assembly: GCF_015290985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Liu,B.-T. and Su,W.
  TITLE     Characterization of Klebsiella pneumoniae strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Liu,B.-T. and Su,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) College of Veterinary Medicine, Qingdao
            Agricultural University, Changcheng, Qingdao 266109, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 406.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 06:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,111
            CDSs (total)                      :: 5,988
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 72 of 191
            Pseudo Genes (incomplete)         :: 125 of 191
            Pseudo Genes (internal stop)      :: 38 of 191
            Pseudo Genes (multiple problems)  :: 40 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028857.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3214"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p7_3214"
                     /geo_loc_name="China:Qingdao"
                     /lat_lon="37.09 N 121 E"
                     /collection_date="2017-03"
                     /collected_by="Weiqi Su"
     gene            complement(join(3908..4096,1..81))
                     /locus_tag="DBX64_RS30335"
     CDS             complement(join(3908..4096,1..81))
                     /locus_tag="DBX64_RS30335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            163..396
                     /locus_tag="DBX64_RS30340"
                     /old_locus_tag="DBX64_31505"
     CDS             163..396
                     /locus_tag="DBX64_RS30340"
                     /old_locus_tag="DBX64_31505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            418..645
                     /locus_tag="DBX64_RS30345"
                     /old_locus_tag="DBX64_31510"
     CDS             418..645
                     /locus_tag="DBX64_RS30345"
                     /old_locus_tag="DBX64_31510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1175..1384)
                     /locus_tag="DBX64_RS30350"
     CDS             complement(1175..1384)
                     /locus_tag="DBX64_RS30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1388..1873)
                     /locus_tag="DBX64_RS30355"
     CDS             complement(1388..1873)
                     /locus_tag="DBX64_RS30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1908..2612)
                     /locus_tag="DBX64_RS30880"
                     /pseudo=""
     CDS             complement(<1908..2612)
                     /locus_tag="DBX64_RS30880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2551..2874)
                     /locus_tag="DBX64_RS30365"
                     /old_locus_tag="DBX64_31520"
     CDS             complement(2551..2874)
                     /locus_tag="DBX64_RS30365"
                     /old_locus_tag="DBX64_31520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3513..3671
                     /locus_tag="DBX64_RS30885"
                     /old_locus_tag="DBX64_31530"
     CDS             3513..3671
                     /locus_tag="DBX64_RS30885"
                     /old_locus_tag="DBX64_31530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     Typing          complement(2983..3061)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          3137..3625
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(1066..2561)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagatttc tttccgaaca gtaaaacagc ctctttttct ccatgctctg tcagcaataa
       61 tcgaaattct tgtccattca atcctctttt cgcatgaaaa aacgacctat acttagatgg
      121 ttagtgacca tatccacttt acctttaagc agggaaccta ttatgaataa aaaaacgctg
      181 ttggcggcca tgatggtcgc gttggtgacc tttgccgctg ctggatgttc ttcaaaccaa
      241 gaaataaaaa ccacagatga tcgcactatc gttacagacg gcaaacccaa agttgacagt
      301 gatacgggat tagtatccta caaagatgcc catacaggcc gaaccgaaca gatcaaccgt
      361 gacaaaatta ccaacatgag tgaactagac aactgatacg gataaaagga gctggctttg
      421 aaaaagattt ttgtttcagt gttcgcagcg gcagttgcgc tttcagccct aaccggctgc
      481 acgcgcacaa gctatgccat ccacaccaac gacgggcgta caattgtcag cgacggcaag
      541 cctacagaat cagactcagg cctgctgggc tataaagacg caaatggtgt caaacagcag
      601 atcaataaag ccgacgttaa agaagtctct gaaattccgc attgaggcat cgctctctcc
      661 gaaaagggcc gctaatgcgg cccttttctt tatctgcccc aacaacaggc aaaaaaagcc
      721 cccgctgggg gccaaatacc tgcagtggct ggatagtact gcattcctga tgaaggagac
      781 gaataagtat gggccggatt tttgcgtctg caggctcggt cgccacaatt tttccgtacc
      841 cgccaggcat catgctttta ccataacatc ccttgcccgc acctctccgg acgggttcag
      901 cgcgaatgcg gcgtcagtcc tgcaaaaccg acagtaaacc accccgcaaa ccgtgcattc
      961 tgcgcctgat aaatctcacc cgactgcata gccatgcagc cgggtgaatg gcaccgggtt
     1021 tcggcgcaga tcctcaatga aagccccgtt ttatccggtt atcacagcga cggaccgtca
     1081 tactcctgct gacgctgcga tcgttccagc tccagcgctt tctggtgctg cgctgcgcgc
     1141 tcctgctccc ggtgtgcctc gatgtcgttc agccttacga caaccggcgc agctgctcgc
     1201 ttaaactcag cacctgcgtg ctcaagtccg tgacgcgctc gctcagcgct gcgttctccc
     1261 gttgcataag accacacatc gtccgccatt ccgcgaaggc gctctcccac tcgtccagcc
     1321 ttttcgagta gtcctgctgt agctgctcta atgcgctcag caactgtttt tccagttccg
     1381 tcatgtgcta tttccccgcc agtatcatcc agctcgaatc ctctccgaac cgtcccgcgt
     1441 ccgggttcac cctcacgcag cggcgtcgtt cctctccgca ggtcgtgagc tgcacgccgc
     1501 tgttcctctc ggacagcatg gcctgcgtgc tcttctgctc ccggatggtc gtgtaattgt
     1561 cgagtatctg ctgcccctgc caccatagaa tgcccgcact tgaggctatc agcagcacgg
     1621 agaccaggac gatggtcagc cacgtctggc tgaccatgcg cagcatccct ttcgtacgct
     1681 ggctcatggc tgaggacagc atccggtcgt ggtcgtggat ggcggcgctg attctcttct
     1741 cgctttcgtt cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca
     1801 tctcgccggt agtctgctgc tgcactttcg atttctgctc taagtccttc gccagcgtta
     1861 aaagactgtt catagatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca
     1921 atgctgatgc tgctttttgt ggtgcgaacc acctcaaaac ctgcgctttc cagcgcctca
     1981 gtgacgtcct gacgcgtttt cagctccccg gacgaggcta aggccagtaa accccgcgta
     2041 atcgcctgag cggcttcctg cttcgtttcc ggcagcgcgg acggcgtgac cagtgcccgc
     2101 cggttttccg gcgcgttcgg gtcgtgcagc cccagactgc cgttcacgat ggtctgccag
     2161 gcatcaatgc gaggacggtc ggcgcggtca tagtatggct ggagccgttt gccggtcagc
     2221 agctccgtgt tcgggatcag gaagttcagc tccagccgcc ccttgtcccg gtgctcaacc
     2281 cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc gaagctcgcc
     2341 atcagcgttt cacgctgccc gggcggtaag tcctgttcgg caaaggacag caccccggag
     2401 gtgtactttt tggcgtaggg cgaggcgtcg ataagctccc ggacttcctc cggcttcccc
     2461 tgaagaacgc tggcaccgtc gcgctggcgg tctttcccca gcagataatc aaccgggccg
     2521 gcaccgcctc cgcgtccccg gggatgaaac ttaacgatca tcgtcagccc ccttttccag
     2581 tacggcatgc cgcagccgct cgagtccggc atcgatggcc atcagcgcgg cgacgacctg
     2641 cacgcggtcg agaccgctac tgccaccggc gttaacctgg cgggcaatct ggttgaggtt
     2701 gttccccatg ccggcaagct gacgaagcag cgccggcgag atcgagggaa gtttgccggc
     2761 ccgggcgggc ttctcgtcca ggcacgtctg ccgcatccag gccgcgagct gtttaccgtc
     2821 gcagcgttcc agcagacggc ggtgctcatc ttctgttacc cacatcgtga gcattttgct
     2881 gcgtttgtct gccaatccgg cctcctgata acgccgggac gggcgggaaa tcttttcccg
     2941 gtcggcgcgg tgttgggcaa gccgcaggcg cgtcagtggc atttagcggg tttcggggcg
     3001 cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttacta tggtggcacg
     3061 acgggaatcg ggatgaaata gtcagtcatg aaaactgtga gatgaggctc aggtgcgtaa
     3121 gaggctgatg cctgtcccgg tggatattcc gcttcctcgc tcactgactc gctacgctcg
     3181 gtcgttcggc tgcggcgagc ggtacggact cacggataaa gacgtggcgg ggaaatacgt
     3241 gaatgcagga ggggaagaaa ggatgcgccg ctgccggttt tccataggct ccgcccccct
     3301 gacaagcatc acagaatctg acgctcaaat cagtggtggc gaaacccgat gcggcttaaa
     3361 gataccaggc gtttccccct gatggctccc tcgtgctccc ccgcattcct gcctttcggt
     3421 ttaccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg acactcagtt
     3481 ccgcgaaggc agttcgctcc aggattgact gtatgcacga accccccgtt cagtccgact
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacac gaaaaatcgc
     3601 tactggcagc agccattagt aactggaggt atacgagaga caaattaaga ggtcttgaag
     3661 tatgggcctg agagaggcta cactgaaaga acagttttgg tttctgcggt ctcgtatacc
     3721 cagttacctg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
     3781 gtggtttttt cgttttcaga gcaagagatt acgactgtaa cgacaggatc acaagaagat
     3841 catttactca gagagactaa ctaccactat gtttgcctat caacatactt aggaaacatt
     3901 acttgagtta acgactccac cttactttta gtatctcgca atgaatcttc cgaccgccgt
     3961 tgtgttgaag ttctattgcg ccaccggggt ataaatcact ttcgtttaac tcaattaaaa
     4021 gctcgttctt gtgagcatcc tctaaataaa ggtaataaca cccaccataa aggttctgcg
     4081 tatctggcac ggcaac
//
