LOCUS       NZ_CP028803             5897 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP7E2 plasmid p3_085072, complete
            sequence.
ACCESSION   NZ_CP028803
VERSION     NZ_CP028803.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06671971
            Assembly: GCF_002258055.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP085072
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 5897)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 5897)
  AUTHORS   Yao,Z., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:04:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,857
            CDSs (total)                      :: 5,735
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 50 of 135
            Pseudo Genes (incomplete)         :: 85 of 135
            Pseudo Genes (internal stop)      :: 18 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028803.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028803.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP7E2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3_085072"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-04"
     gene            103..231
                     /locus_tag="B7D49_RS29370"
     CDS             103..231
                     /locus_tag="B7D49_RS29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256557297.1"
                     /translation="MALKPGPKRIAESTGQPDQRQRDNKKTPGNTDKLKPSKSSGK"
                     /name="hypothetical protein"
     gene            complement(1017..1439)
                     /locus_tag="B7D49_RS00110"
                     /old_locus_tag="B7D49_00125"
     CDS             complement(1017..1439)
                     /locus_tag="B7D49_RS00110"
                     /old_locus_tag="B7D49_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_094620558.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKCIKVID
                     TSELIRVFGPLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1439..1630)
                     /locus_tag="B7D49_RS00115"
                     /old_locus_tag="B7D49_00120"
     CDS             complement(1439..1630)
                     /locus_tag="B7D49_RS00115"
                     /old_locus_tag="B7D49_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3836..5257)
                     /locus_tag="B7D49_RS00120"
                     /old_locus_tag="B7D49_30255"
     CDS             complement(3836..5257)
                     /locus_tag="B7D49_RS00120"
                     /old_locus_tag="B7D49_30255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064169724.1"
                     /translation="MKYFSTGFFLKGNVEVKDIINECLSWVSTSPFTSFVPASLDMKRG
                     LDEFDIEVGDERIELINFSDDSIMMSCFRYSKISGAHKWVTDVAVNYYFSEGKTWVQVE
                     SSVVTQQAVHKAPVTKTPLIVLRLLDKFSGGRDDVFKVGIEPVLLDGSEHSLGFACDII
                     NGRHKNRLPVVYVSSKYFFKEHPHNVIPGRLARKLSGLAHVVVEPEGHLFSNKLKHEVN
                     NKNSYGGSVGIYWPGGQSISIHKRGSLSAAEFEKEIFKEIITAMTSLAPLRKNGWSEIV
                     SSKNRKAIQVLKDNGSQSSELASLYEEENESLQDEVKELNQKIASYEARIRVLMERTSM
                     QGGVFLKIGNEYELFEGEVLEIIMSSIRKVLPTNYEGGRYHDVLSSISSANPCSTNLLE
                     RDKKLKQALVGYTKMTPKVRGVLNDVGFSISEEGKHYKITYHGDPRYTYILPKTGSDYR
                     GALNAYSDISKKVFS"
                     /name="hypothetical protein"
     gene            complement(5364..5630)
                     /locus_tag="B7D49_RS00125"
                     /old_locus_tag="B7D49_00140"
     CDS             complement(5364..5630)
                     /locus_tag="B7D49_RS00125"
                     /old_locus_tag="B7D49_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094620559.1"
                     /translation="MVWMINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRHSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5614..5838)
                     /locus_tag="B7D49_RS00130"
                     /old_locus_tag="B7D49_00135"
     CDS             complement(5614..5838)
                     /locus_tag="B7D49_RS00130"
                     /old_locus_tag="B7D49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="WP_064169727.1"
                     /translation="MLAIRLSDEIESRLNLLAKQTGRTKTFYAREAILTHLDDLEDYYL
                     SAETVARVRRGDEVVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     Typing          complement(2971..3150)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.946"
                     /coverage="83.0"
                     /evalue="3.5099999999999995e-46"
     Typing          3100..3609
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 atcaaagaac aaaaaacaat ctttccattc atcttgacta cctacaactg tcgatatatg
       61 cagttataga gtttcctctt aacacgatga aggatatcca ttatggctct taaacctgga
      121 ccaaaacgaa ttgcagaatc aacagggcag cctgaccagc gccaacgcga caataaaaaa
      181 actcccggaa atactgacaa gttaaaacca agtaaatctt caggaaagta gttgttagca
      241 aagcccttag gggctttttt ttgtacttgt cggtcagacg gattaaacat aacatccgtt
      301 gcgtgcaccg cctgagaggc tctcagggcg tttctgacgg gaaaccggta aaacaggcaa
      361 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
      421 tgcgcgcaag tgcatagcgc ggaaattcac gccattcagc cggggtaagt cctgtttccg
      481 atgttcttct tacccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgagacg
      541 ttgccggcac agccggaaac cccgtatcag cacgatcagg aatcgggttc gtcgacggcc
      601 ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgac
      661 tcgtgacttg actctagagt caccgcggtc aaaaaaacgc caaagcgacg attcgactct
      721 agagtcaagt cgtcataagt tactgatagt gtggagtgag tcgtcgatga ctctatgacg
      781 aatcgtcggc aagtcgtaca cgaatcaccg acgacttgga ttgaccttac agaaggtaat
      841 actgcgtaat ttaataacaa ccaactgact gatattaatg agttatctgc aaaaatttaa
      901 ttttgcgtgg ggtgtggata tttttagtgt gaggagcgtt cagagatctg gcgatttttg
      961 ggaaggttca cgaacgcccc ggcatggccg gagaggaaaa ctcaagcatg gcgggatcag
     1021 gattttttcc agaatttcca ccacggggaa tggggtggtg aagaaggttc gtgcttgtgc
     1081 tccagaagct gcatagcctg acgaagggaa tcaatatgct catctcgagc cttaacagcc
     1141 cctttaagac catcgttttc agcttcaagt aaagcgattc tatgttgtaa atcatgtaaa
     1201 ccaacgctgt taatacctgt agattctgcg tcacttttta catcatattt tacagcatcg
     1261 attacaccat caagaggacc aaatactcta ataagctctg atgtatcaat aactttaata
     1321 cacttcccgt gctttacaac agaggtgtaa cttaacttac cactgttaat catcttgtaa
     1381 atagtagggc gactaacctt aacaagccgt gctgcttcac taatagacac tctggccatc
     1441 agttaccatc ctgtaatgca tccagtctcg aggacagtgt acggaaaaga tgctccgcat
     1501 catcgtggag tttctcacaa aggtcggcct cagcatcgag atcaagctcg ttaagcttct
     1561 ccagcaggag cagagactga ttctggatga atttagccat gttcagcgct gttttctgtt
     1621 gtttcgtcat aagggacctc agagcatcag tgctcagtgt aaaaccggta agggggtcgg
     1681 atgtacgaat ggttcagaga acggatctga ggtaatcctg tagttatttt gtagtttatc
     1741 tgtagtcatc gcactaaaaa tcaggattaa gtcatcagct tttgtagtct ttctgtagtt
     1801 tacctgtagt ccggaaaaat aatccgttca tgcgagcatg aacgaggtcg ttaatgacgt
     1861 ttttttgtca ccgacgcccc ttaacctccg cagatcaccc ggccagaatg ataatttttt
     1921 ggcccttaaa tggtcataca cctaacgcag ctgcgatttt catcgcaact gcatgaatta
     1981 aaaggtaaaa actgaaatct ccgtttattt atgtaatacg caacgtatta caatcacctg
     2041 taatcgcatg aaaataaaga aataaaatgt aataccggcg tattaccacg tacgcgggca
     2101 agcgaaaagc tataacgctt cccggccgac aggcggcccg gaatccacag gggcatgcag
     2161 gttctcttca gtaacctgcg ccattaaatc aaaaaggtca tattcgccgg gaagctgcgt
     2221 atcaggggtc agtttggata tagagaatta ttgtacggta agcctgtttt ttgaacagac
     2281 ttacctatag caatagggtg tactcccgcg ctatacggca ttttcagagg tttgacctta
     2341 atcggataac ctaaatgcga tactgcaaga gcgacaactt aaaagcgata gccactgacg
     2401 caaaatcaac cccttacgaa ccaagattgg ttaaataatg cgcttaacgt acaaaaattt
     2461 ccgatctcca aactgacccc tactccgaaa gcgcagtaga aaaaaggcaa aacctgctct
     2521 ggagtacagt cagaagaacg gaaaaaaacc gggcggctag cccggaaggt aagtcaagtt
     2581 agattggaac aatcttccgt gacataggac atgtcacaac gaccatcatc catgacggcc
     2641 gcctgacaca cagtatggtt caaaaatcag aaaaaaaact cagacggtga catatttaat
     2701 acgaacagat tcaaggaacg ccaacatgca ctccctgacc cggggatctg tctcagataa
     2761 gaccagatag taagcatcca gcatcatttt gccggagtct cccccagact ggcgtaagca
     2821 gtcagaaatc actgacacag ccagctgccg ttcagcatca ctcatattca tcaggatacc
     2881 agaggaaaaa tcaaacaagg aaacctcagt taacggcagc cgaaagaaaa tattgacagt
     2941 ttagcagtaa aaaaacaaaa agacggtcct tcgcgggttt cggggcgcag ccctgaacca
     3001 gtcacgaagc gctagcggag tgtatactgg ctcaatatgt tacggcggag taacacttaa
     3061 taaaagtgct tcatgtgaca ggggaaaatg cagctacagg gcgtaaggaa gaaaatggga
     3121 gttacggtga tatgacgcct cctcgctcac tcggtcgctg ccgctcctgc cgttcggctg
     3181 cggcgggcgt tacctgcaca cggataagag caaaggcata cggcatgaca agctgggcaa
     3241 aaaaaggata cggcgctgcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     3301 atctgacgct caaatcagtg gcggcgaaac ccgacaggac tataaagatc ccaggcgttt
     3361 ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     3421 ccgctgttat ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggaaggcagt
     3481 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     3541 cggtaactat catcttgagt ccaacccgaa aagacacgac ttaacgccac tggcaacggc
     3601 cactggtaaa cagaatgtgc gagagatgta tcgagagttc ttgaagtggt ggccttactg
     3661 aggctacact ggaaggacag atttggtgac atgtctcgca caagactgtt accacggtta
     3721 gagaatctgc caggattaac taaccttcga aaaaccacct gccagggtgg ttttttcgtt
     3781 tcaaaactaa gatacatcca gcaagagatg aactcaagca ccatgtctta ctggtttatg
     3841 aaaacacctt ttttgatatg tcggagtaag catttaacgc ccctcgatag tcactccccg
     3901 ttttaggtaa aatatatgtg taccggggat ccccatggta agttatttta tagtgcttac
     3961 cctcttcgct tattgaaaaa ccgacatcat tcaaaacacc tctcactttt ggcgtcattt
     4021 ttgtatagcc tactagcgct tgcttcaact ttttatctct ttccaagagg tttgtactgc
     4081 atgggttagc ggaagatatt gaagatagaa catcatgata tctacctccc tcataatttg
     4141 tcggaagaac cttcctaatt gaagacatta ttatttctaa tacctcacct tcaaacaact
     4201 catactcatt gccaatcttc aaaaaaaccc caccctgcat agatgttctt tccatcaata
     4261 ctcttatcct tgcttcataa gaagctatct tttgatttag ctcctttact tcatcttgca
     4321 gggattcatt ctcttcttca tatagagaag cgagttcact actttgactt ccattatctt
     4381 ttaaaacttg aatggctttt ctgtttttac tactgactat ctcagaccac ccatttttcc
     4441 ttaagggggc cagtgaagtc atagcagtta taatctcttt aaatatctct ttctcaaatt
     4501 cagcagcact aagactacct cttttatgaa tactaatact ttgccctccc ggccagtaaa
     4561 taccaacgct tccaccataa gaatttttat tattaacttc atgcttgagt ttattagaaa
     4621 acaagtggcc ttcaggttct acaaccacat gggccaaccc tgatagcttc ctagccaacc
     4681 tgccaggtat cacattatgc ggatgttctt tgaaaaaata tttagagctc acataaacca
     4741 caggaagcct gtttttgtgc cttccattaa ttatatcaca agcaaagcca agagaatgtt
     4801 ctgaaccatc cagtaaaaca ggttcaattc ctactttaaa aacatcatca cgcccaccag
     4861 agaatttatc tagcagacgc aatacaatga gtggtgtttt agttacaggt gccttatgaa
     4921 cagcttgctg agtaacaaca ctagattcaa cttgaaccca ggttttccct tctgaaaaat
     4981 aataattaac agcaacatcg gtgacccatt tatgtgcccc tgatatttta gagtaacgaa
     5041 aacaagacat cataatcgaa tcatctgaaa aatttatcag ttcaattctt tcatcaccca
     5101 cctcaatatc aaactcatcc aaccctcttt tcatatctaa gctagcggga acaaatgaag
     5161 taaatggtga tgttgacacc catgacaaac actcattgat tatatctttt acctcaacat
     5221 ttcctttcaa aaagaagccc gtggaaaaat atttcataaa caccccttat gatttagagt
     5281 aaaatatttt ttaaaattaa aaagccattt tgttaacgac atatttttca cgcctcacat
     5341 aaaaaaaacc cgcaaagcgg gcttcagtcg tagacttcac gtcggtggcc aatggtagca
     5401 gccagaataa tcagctcgtc gtccctgatc tcgcatagga tgcgataatc gccaactcga
     5461 tagcgccaga attctccaag ctcaccttta agcggcttac ctgagtggcg tggatctgcg
     5521 gcaacggcaa tacgtaaatc cataaaatcc acaatccgtc gtgcgttctg cttgtccatc
     5581 ttacgtaacg acttgagcgc tcggtcggaa tagttaatca tccagaccaa gcgacttcct
     5641 cacgtcttca gacgaatgta caacttcatc cccacggcga acacgtgcca cagtttctgc
     5701 tgaaagataa taatcttcaa gatcatccag atgcgtcagg atcgcttcac gcgcatagaa
     5761 cgttttagtc ctacctgttt gcttcgcaag caagtttaga cgggactcaa tctcgtcaga
     5821 caatctgata gccaacataa agacctctct gaatccaaat tgatatacat gtataacaaa
     5881 attatacaac attaaaa
//
