LOCUS       NZ_CP028802             6425 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP7E2 plasmid p2_085072, complete
            sequence.
ACCESSION   NZ_CP028802
VERSION     NZ_CP028802.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06671971
            Assembly: GCF_002258055.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP085072
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 6425)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 6425)
  AUTHORS   Yao,Z., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:04:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,857
            CDSs (total)                      :: 5,735
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 50 of 135
            Pseudo Genes (incomplete)         :: 85 of 135
            Pseudo Genes (internal stop)      :: 18 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028802.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028802.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP7E2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_085072"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-04"
     gene            12..353
                     /locus_tag="B7D49_RS00070"
                     /old_locus_tag="B7D49_00085"
     CDS             12..353
                     /locus_tag="B7D49_RS00070"
                     /old_locus_tag="B7D49_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            562..>990
                     /locus_tag="B7D49_RS29365"
                     /old_locus_tag="B7D49_30230"
                     /pseudo=""
     CDS             562..>990
                     /locus_tag="B7D49_RS29365"
                     /old_locus_tag="B7D49_30230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1031..1528
                     /locus_tag="B7D49_RS00080"
                     /old_locus_tag="B7D49_30235"
     CDS             1031..1528
                     /locus_tag="B7D49_RS00080"
                     /old_locus_tag="B7D49_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1535..1750
                     /locus_tag="B7D49_RS00085"
                     /old_locus_tag="B7D49_30240"
     CDS             1535..1750
                     /locus_tag="B7D49_RS00085"
                     /old_locus_tag="B7D49_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2202..3452
                     /locus_tag="B7D49_RS00090"
                     /old_locus_tag="B7D49_00075"
     CDS             2202..3452
                     /locus_tag="B7D49_RS00090"
                     /old_locus_tag="B7D49_00075"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            3445..4116
                     /locus_tag="B7D49_RS00095"
     CDS             3445..4116
                     /locus_tag="B7D49_RS00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="WP_179236698.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVREGANKFL
                     I"
                     /name="endonuclease"
     gene            4157..5137
                     /locus_tag="B7D49_RS00100"
                     /old_locus_tag="B7D49_00110"
     CDS             4157..5137
                     /locus_tag="B7D49_RS00100"
                     /old_locus_tag="B7D49_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019473.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            5119..5454
                     /locus_tag="B7D49_RS00105"
     CDS             5119..5454
                     /locus_tag="B7D49_RS00105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220541998.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVSL
                     GRNDLLIRTFLRCRAISAQFVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDI
                     SNYRMSV"
                     /name="hypothetical protein"
     gene            complement(5674..5805)
                     /locus_tag="B7D49_RS29090"
                     /old_locus_tag="B7D49_00095"
     CDS             complement(5674..5805)
                     /locus_tag="B7D49_RS29090"
                     /old_locus_tag="B7D49_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     Typing          6178..6353
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          complement(5697..6195)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.04"
                     /coverage="92.0"
                     /evalue="5.84e-177"
     Typing          344..1866
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggaggcgat tctggcagat aaacgcaaca agatgctcac gatgtgggtg acggaagacg
       61 aacaccggcg gttgcttgag cggtgcgacg gaaaacaact ggcggcgtgg atgcgccaga
      121 cctgccttga tgagaaaccg acacgcgccg gcaggcttcc ctcgatttcg cccgcgctgc
      181 tgcgtcagct tgccggcatg ggcaataacc tgaaccagat cgcccggaaa gtgaacgccg
      241 gtggtgccgg tcatgaccgg gtgcagatcg tggcagcgct gatggcgatc gatgccggtc
      301 tggaacggtt gcggcatgcc gtactggaaa aagggccgga cgatgattgt taaattccat
      361 ccccggggtc gcggcggggg cgccggtcct gtggattatc ttctgggtaa agaccggcag
      421 cgtgaaggtg ccagcgtcct gcaggggaag ccggaagaag tccgggagct gattgatgcg
      481 tcgccgtatg tgaaaaaata tacctccggg gtgctgtcat tcgcggaggc ggacctgccg
      541 ccgggtcagc gtgaaaaact gatggccagc tttgagcggg ttctgatgcc cggactcgat
      601 aaagaccagt acagcattct gtgggtggaa cacgcagaca agggtcgtct tgagctgaat
      661 tttctgatcc ccaataccga attactgacg gggaaacgcc tccagccgta ttacgatcgt
      721 gccgaccggc cgcgcatcga tgcgtggcag acagtggtca acggcaggct ggggctacat
      781 gatccgaacg acccggagaa ccggcgggcg ctggtcacgc cttcaggact gccagaaacg
      841 aagcaggaag ccgcagagac gattacacgg ggtttgctga tgttagcctc atcaggagag
      901 ctgaaaacgc gtgatgacgt cacaggggcg ctcacagcgg ccggttttga ggtggtcaga
      961 accacaaaaa acagtatcag cattgccgac ccggacgggg ggcgaaatat ccgactgagg
     1021 ggagccattt atgagcagtc ttttaacggc ctcgagggac ttcgaggaga aatcgaaagc
     1081 acagcagcag cttaccgaag agaggctgaa agccgcattc agcaagcacg agaacgttgt
     1141 caaatcggaa ctgaacgcaa gcgcgaagag aatcaacgac gccatcagcg cccacgaaca
     1201 ggggatgaac gcggcgatgc agtcgaaccg gctgagcgtg ctgcgaatgg tcgggcggac
     1261 gtggctgacc atcaccatgg tgtcagggct gctgttcgcc agcctgagcg gggtgctgtg
     1321 gtaccagggg aaccggatag cggcgaacct ggcggaaatc aggcagcaga gagatacgct
     1381 gtcgaaactg cacatgcaga cgtggggcgt gacctatctg gagaaccgca acggacggtt
     1441 tctggtgctg ccggagggga tgaaagccga aacgggccgg acggtggaca acaagacgcg
     1501 gaacgcggtg aagctggtac gggagtagag cagaatgacg gaactggaaa aacaattgtt
     1561 gagcgcgtta gagcagctac agcaggacta ctcgagaagg ctggacgagt gggagagcgc
     1621 cttcgaggaa tggcggaaga tgtctggtct tatgcaacgg gagaacgcgg cgctgagcga
     1681 gcgcgtgtcg ggcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttatc
     1741 gacaggctga atgagattga gcaacagcga tacgggcaaa caacggaaaa ggaaccagag
     1801 aagacagcag atctggtacc ggaacggcaa cgcacattat ctcatggtga cagggagtat
     1861 ggagactgat aaacgcccgt ctcaggtggc ctgagcgtga cggggatcat ggccattttc
     1921 tgcgatttca gcacacagag agtctttttt aaccgaaatc accggctatg cagtgatgtg
     1981 aataagtgat gcatatgcgt aaaataaatc aggtgcgttt ctggtggttt tcggtgtact
     2041 tgccaccacc ttttgcccgt cagccatcag aaacgccctg agtgcctccg gcgcggtgcg
     2101 ggtaagagat gttatgttca atcgaaatca gaaaaacgat cggtaaagcg atcgtttttc
     2161 tgatagcatc atgttgattt tatataacgt gggtgtaaag aatgactaga ccgattgctg
     2221 ttgatttatt tgctggagcg ggtgggctga gtctcggttt cgagcaagct ggctttgata
     2281 taatggcatc agttgaaata gatccaatac attgctctgc tcacaaatat aactttccta
     2341 tgtgcaaaac gatatgtaga agtgttgttg atgttaccgg agaatacgtt agggaagttt
     2401 caggtattgg tgagaaagag attgatgtag tgatcggagg cgctccctgc caaggctttt
     2461 cattaatagg aaaacgcgcg cttgatgatg atagaaacaa actggtctat cactatgtac
     2521 ggctggttct tgagctaaag cctaaatact ttgtttttga aaatgtaaaa gggttgacgg
     2581 ttggtaagca taagcagttt ttagtagaga taatggatgc atttaaagat ggcgggtacg
     2641 acgttgtaag cgattataaa gtgctaaacg cagctgatta tggagttcct caagatcgcc
     2701 gtcgtctttt cttaatgggg ggaaggaaag gaattgctct gccatcatac cctgagccat
     2761 tatccattag aacaacggtt ggggacgcta ttggggatat tcctgatgcg gaaaaatatc
     2821 ctgagctatg gggtagagat tgggtttatg ctaaatttgg tgagcaatca ctatactcat
     2881 cctatttgca tggcgtagga aaagatccta gtgattttag ctacccaagg atttttaata
     2941 aagacaaatt aacgtgttct ttactgacag atcactctga catttctaga gggcggtttg
     3001 ttgaaacttt gcctgacacg gtagaaccaa tcagcaggtt taaaaagctc tcgatgaatg
     3061 ggctatgtaa tactttaagg gcaggaacag caagtgacag aggtgctttt acatctcctc
     3121 gtcctataca tccgatctat cctagggtca tcacggtcag agaggcagca agactgcatt
     3181 catatcctga ttggttcaga tttcatgtga ctaagtggca tggatttaga caggtcggta
     3241 atagtgttcc tcctatgcta gctagaagtg ttggcttgca gattatgaag gttcttggtg
     3301 ttacgccaaa aaaaccaacg aagaaaatca aactcggtga tgaaagtttg gttgctattt
     3361 ctatgtccga tgctgcatct atgtttggtg tgtcatcaaa tgtaattgct aagagaataa
     3421 aaaatagtaa caaggaggtt gtttgtgagt aaggataaaa aagaaaggtc caaaaataga
     3481 taccatcagt tggtagaaaa gatatttttc gataaatatg ttgatggtga cttagaggtt
     3541 ttgtttgaaa ggttagatat aaacagtgct gcaaaagatt taggtataga actcccatct
     3601 aatgttggtg atgttatata ttcaatcaga tttagaacgc ctatgccaga aagtattctg
     3661 ttgactcagc cagaaggtaa ggagtggatt attgagctgg ctggtaggtc aaagtatcga
     3721 tttagattag taactattag taggattttg ccaaatccta atatggtggc aataaaaatt
     3781 ccagacaata ccccggagat aattacaggt tattctttag acgacgagca ggcattgctg
     3841 gcgaaggtga gatataaccg gctaatagat atatttctag gtataaccac atattcttta
     3901 cagaatcatt taagaactac ggtgaaggga attggtcaaa tagagatcga tgaactttat
     3961 gtaggtatag ataaatacgg ttgccattat ataataccag ttcaagcaaa aggtgggaca
     4021 gatcaaatat caatggtgca aacatcgcaa gatacagcgt ggtgtgaaca aaaataccca
     4081 aatgttaggg aaggtgcgaa caagttcctg atatgagatc atcatattca tccggagcgc
     4141 atcccagagg gacatcatga gccatcaact caccttcgcc gatagtgaat tcagcactaa
     4201 gcgccgtcag acccgaaaag agattttcct ctcccgcatg gagcagattc tgccatggca
     4261 aaacatggtg gaagtcatcg agccgtttta tcccaaggcg ggcaatggcc gacggcccta
     4321 tccgctggag accatgctgc gtattcactg catgcagcat tggtacaacc tgagcgacgg
     4381 tgccatggaa gatgccctgt acgaaatcgc ctccatgcgc ctgtttgccc gattatccct
     4441 ggatagcgcc ctgccggatc gcaccaccat catgaatttc cgccacctgc tcgagcagca
     4501 tcaactggcc cgtcaattgt tcaagaccat caatcgctgg ctggccgaag caggcgtcat
     4561 gatgacccaa ggcactttgg tggatgccac catcattgag gcacccagct ctaccaagaa
     4621 caaagagcag caacgcgatc cggagatgca tcagaccaag aaaggcaatc agtggcactt
     4681 tggcatgaag gcccacattg gtgtcgatgc caagagtggc ctgacccaca gcctagtcac
     4741 caccgcggcc aacgagcatg acctcaatca gctgggtaat ctgcttcatg gagaggagca
     4801 atttgtctca gccgatgccg gctaccaagg agcgccacag cgcgaggagc tggccgaggt
     4861 ggatgtggac tggctgatcg ccgagcgtcc cggcaaggta aaaaccttga agcagcatcc
     4921 gcgcaagaac aaaacggcca tcaacatcga atacatgaaa gccagcatcc gtgccagggt
     4981 ggagcacccg tttcgcatca tcaagcggca gttcggcttc gtgaaagcca gatacaaagg
     5041 gctgctgaaa aacgataacc aactggcgat gttattcacc ctggccaacc tgtttcgggt
     5101 ggaccaaatg atacgtcagt gggagagatc tcagtaaaaa ccggaaataa cgccagaaat
     5161 ggtggaaaaa atagcctaaa taggctgatt cgatgtgttt gcgggaaaaa aatcggccca
     5221 gatccgcgaa attttaatca gcgagtcagc ttgggaagaa atgacctgct tattcgcacc
     5281 ttccttagat gtagagctat ttccgctcag tttgtttcga gcgatttgat tgcactgttt
     5341 gagctaaaga tagacgaagg acaagtgaaa ttgattgagg aaaggcatta caaactggtt
     5401 ccggtaaaag agttagatag ctctgacatc tcaaactacc gcatgtctgt ataatttctg
     5461 agaggatctt cttgagatcc tttcgttcta gccgtaatct cctaagccgt aaacaaaaaa
     5521 accaccgcta ccaacggtgg ttcgtttgcc ggaatttaga gctaccaact ctttcttccg
     5581 aaggtaactg gctttcacgg agcgcagata ccaaaactgt cctttcagtg tagccgtagt
     5641 caggccacca cttcaagaac tctgtagata actctactcc gctccgtgaa tccgattacc
     5701 agtggctgct gccagtggcg cttttgcatg tcttttcggg ttggactcaa gacgatagtt
     5761 accggacaag gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     5821 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     5881 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagctaccag
     5941 ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     6001 agattttgtg atgcttgtca ggggggcgga gcctatggaa aaacggcaac gccgcatcct
     6061 ttttcctgac tttcctgact ttcctgactt tcctgacttg agcgcgatct cgttatctcg
     6121 tccttcgtga gctggtaacg cccgccgcag tctcacggca ggagcgcagc gaccgagtga
     6181 gcgaggaggc gtcatttcat cgttacctgc attttgcact tacgccccgc agccgtattt
     6241 tcccccttgc cacatgaagc acttccccga atctccatct cgccgtaaca tactaagcca
     6301 gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa ccccactgac
     6361 gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg ctttcccgtc cgtcacggcg
     6421 ttatc
//
