LOCUS       NZ_CP028801            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP7E2 plasmid p1_085072, complete
            sequence.
ACCESSION   NZ_CP028801
VERSION     NZ_CP028801.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06671971
            Assembly: GCF_002258055.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP085072
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Yang,B., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 10060)
  AUTHORS   Yao,Z., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:04:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,857
            CDSs (total)                      :: 5,735
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 50 of 135
            Pseudo Genes (incomplete)         :: 85 of 135
            Pseudo Genes (internal stop)      :: 18 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028801.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028801.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP7E2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_085072"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-04"
     gene            complement(join(8679..10060,1..583))
                     /locus_tag="B7D49_RS00005"
                     /old_locus_tag="B7D49_00055"
     CDS             complement(join(8679..10060,1..583))
                     /locus_tag="B7D49_RS00005"
                     /old_locus_tag="B7D49_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2067..2216)
                     /locus_tag="B7D49_RS00010"
                     /old_locus_tag="B7D49_00065"
     CDS             complement(2067..2216)
                     /locus_tag="B7D49_RS00010"
                     /old_locus_tag="B7D49_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2301..2558)
                     /locus_tag="B7D49_RS00015"
                     /old_locus_tag="B7D49_00070"
     CDS             complement(2301..2558)
                     /locus_tag="B7D49_RS00015"
                     /old_locus_tag="B7D49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(2568..4253)
                     /locus_tag="B7D49_RS00020"
                     /old_locus_tag="B7D49_00005"
     CDS             complement(2568..4253)
                     /locus_tag="B7D49_RS00020"
                     /old_locus_tag="B7D49_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4580..4825
                     /locus_tag="B7D49_RS00025"
                     /old_locus_tag="B7D49_00010"
     CDS             4580..4825
                     /locus_tag="B7D49_RS00025"
                     /old_locus_tag="B7D49_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5099..5470
                     /gene="tolA"
                     /locus_tag="B7D49_RS00030"
                     /old_locus_tag="B7D49_00015"
     CDS             5099..5470
                     /gene="tolA"
                     /locus_tag="B7D49_RS00030"
                     /old_locus_tag="B7D49_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5467..5832
                     /locus_tag="B7D49_RS29085"
     CDS             5467..5832
                     /locus_tag="B7D49_RS29085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6007..6285
                     /locus_tag="B7D49_RS00040"
                     /old_locus_tag="B7D49_00025"
     CDS             6007..6285
                     /locus_tag="B7D49_RS00040"
                     /old_locus_tag="B7D49_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6273..6587
                     /locus_tag="B7D49_RS00045"
                     /old_locus_tag="B7D49_00030"
     CDS             6273..6587
                     /locus_tag="B7D49_RS00045"
                     /old_locus_tag="B7D49_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6751..7179
                     /locus_tag="B7D49_RS00050"
                     /old_locus_tag="B7D49_00035"
     CDS             6751..7179
                     /locus_tag="B7D49_RS00050"
                     /old_locus_tag="B7D49_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7205..7384
                     /locus_tag="B7D49_RS00055"
                     /old_locus_tag="B7D49_00040"
     CDS             7205..7384
                     /locus_tag="B7D49_RS00055"
                     /old_locus_tag="B7D49_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7411..7941)
                     /locus_tag="B7D49_RS00060"
                     /old_locus_tag="B7D49_00045"
     CDS             complement(7411..7941)
                     /locus_tag="B7D49_RS00060"
                     /old_locus_tag="B7D49_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7948..8679)
                     /gene="mobC"
                     /locus_tag="B7D49_RS00065"
                     /old_locus_tag="B7D49_00050"
     CDS             complement(7948..8679)
                     /gene="mobC"
                     /locus_tag="B7D49_RS00065"
                     /old_locus_tag="B7D49_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9253
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1386..1964
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7952..8679)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cctgattcag tttcgcgttc accgcctcgt cagcggccgc aaaatgacca tcaagcagca
       61 ggaacgccag cagccgcgcc agaaggacgc cggcgacaag ccctccggca caccaggcta
      121 tcccgaacag gttagccggc tgcagcaggt aataatctgc gttcgcatag aggtccgggc
      181 gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc cccagaaggg
      241 ccagaccggc agcgccggtc agccagtacg cgtagttcca gacatgtcgc cgtgccctga
      301 ccatccgccc gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc cggttattat
      361 cgcgcttata gcgatcggca tactcccgcc gcgccttatg gctcagcccc acaaaacgca
      421 ttcggccggc ccagagttca gccagaccga aaaacaggcc gataaccaga aaaaaaccgg
      481 ccagactgta atactcccgg tacagccagc tgacccacgg gtaaatctgc cgggccagct
      541 ccgggatatg ccactgccag gcaagcgaat ccgtattaaa catgcaatct ctctccttca
      601 gacgacgggt ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg acatgcccca
      661 aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc gcggaggcca
      721 gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag cacgccccag
      781 cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg gcccccgtca
      841 gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt gctgcttcgt
      901 caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg acgggggccg
      961 ggtgaatttc tggcgtggcc ggcgtccagc cggacagagc cagaaagagg ggaaaagacc
     1021 gtgcgtcggt ggcgtccagc cagcgtaagc aaggtctttt cccctgaagt cagccacgcc
     1081 ccagcgggga tgacttgcat ttctccgcaa ggagaaaaaa gcaacagaga taggttaacg
     1141 taaaatgttc agcgaaaaac cgacatggtt cacctatcct gataattgat cgtcagggaa
     1201 tagaaagact taatcagggg acaatatccc acatcagcgc gtccggtgac cgtcttgcag
     1261 gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccgggc gtgagactgc
     1321 ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag caggggacta
     1381 acatgtgagg caaaacggca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc
     1441 tgccagagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg tgaggctcaa
     1501 ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg
     1561 taggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgtccg
     1621 ccgttctccc ttgcgggaag tgtggcgctt tctcatagct cacacactgg tatctcggct
     1681 cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt cagcccgact
     1741 gctgcgcctt atccggtaac tgttcacttg agtccaaccc gggaaagcac gcaaaacgcc
     1801 actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg
     1861 ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg
     1921 ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa
     1981 accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg
     2041 atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag ttcagaggag
     2101 gacgatggtg ccaccgttcc gccctgaaca tcccggatat aatttgcctg acatgcggcc
     2161 agaagaaagc cagcaacaat caatataaaa agaaaaatag tctttacctt tttcatatac
     2221 tttccctgta tttaaagact ccggcattca tccctgaaca ccggaagtca gtatttcaaa
     2281 tcatccttga taattttttg ttaccagtta ccgtcccgat aatcaaaagc tatataacaa
     2341 ttatccacat caggttttat ttgattttta aaaaatggct gtagtaaagg aacccattct
     2401 ttcttcaaat caaactcacc attattaata ttatccttta aaggcattcc caagctttca
     2461 atgacagaac catcgtcacc aaagtcttta gaatactctt cccctttaaa ctcttcagtt
     2521 ttcttatcaa accatgtcaa acgtaattta agccccatat ttacctctca aagatatttt
     2581 ttaatgttcc gttttggatc tggcccttta acctgcttac ccgttttggg atcaaatgcg
     2641 cctaggtgtt cgccatcact ggcacgatac ccttcaagct caccgtgctg ggaatcccat
     2701 tcataaattt tacgtttttt atcgccatac catcgggccc gcttaccacc accaccttgt
     2761 ttaggtgttt taggatcacc tcgctttaac tctccaagtc cctttatgtc ttctgtttta
     2821 ggtactggat gataatcatg gccgtagtct ttagtcccct tgcgaggctt gttcttttcc
     2881 tcatttaact tattttctgc atccttcgct tttttctcct tttgtttccg gctctccatc
     2941 gccccggtaa gtgcagcctt atctctggat aaggattcat tcattgcgtt taaagatgcc
     3001 tgagccgctt ttaatttctc attctgattc tgaatatcct tatcaagata gcgaacctga
     3061 tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc atctttattt
     3121 gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc accggggagc
     3181 gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa tatttttatc ttcggcatcc
     3241 agctcagctt tcgctttatc atagtctctt ttcagcccct cttccggatg agcggcgtcc
     3301 catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt ttacctgtgc cggggtaaga
     3361 acatctgtca ccgacacata aaccggcttc tgtccggtct ctttcgggaa acgaataacg
     3421 gcctcacggg agttaccacc ggctgtaaaa ccagccggac gtgaatcacc tttttcagca
     3481 acaatgcctt ttggcggggc tttcgctgtc ggaacacctt tcggtacgct cacctgcaga
     3541 gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg tttcgcatcc
     3601 accacaggga cactcattgg ccgacctgcg acaaccgcaa tatgctgccg ctcatccttc
     3661 accacatcca caacccgttg tctgacacga accgtcgcct ggtccagtgg taaagtactt
     3721 gccggagtct ccgtcaccgc atcggccggt aatgacgtca taatttttga catcattttc
     3781 ggatcatctt ttgcaatctc agaaggcagc acaccgtaga tcgcaatccc ccacagacca
     3841 aacttaaacg gccccttcag ggcagccagc acatcagcaa cggcggccga caacgcatcg
     3901 ccggaaactg ataaagccag tccttcagcc cccggagtag ccagggcagg cagaccgaag
     3961 gccatcgcag tggccgaaga ctgtccgcca ccagagctac cgctatgatt gccgctgtta
     4021 ccaccattac caccgggttt actcccgccg ttaccaaact cccccggatt atagttatga
     4081 atatctccat tcggcgtatg cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca
     4141 ccacctgatg aagggccacc tttaccagac gtaccgttca cattcccact ggcctgacca
     4201 ccattatgtc ccagacctga attacccgga cctcgaccgt ctccaccact cataccgttt
     4261 tcctctttga catttaaaac aataaattaa agccaacata ctgtacatat acacagtata
     4321 attattacct tactcctccc gttttcaggt gtcaatatat gagattagtt ctcatcttca
     4381 ggcacaaaaa agcgcccgga gccacctgta agcagatcgc gtcttttccc gaaaatttct
     4441 catggcaaca aattttatta tttaagacat tcatcacact tacatttcag atggtataga
     4501 cacatctgaa agctaactgg catgaggaga ggacctgcgg ttaaactgtt cgggaaacct
     4561 gagacgcccg gaggccacta tgacgcacct gaaactgact cccgctgaag aaaagcgcct
     4621 ggcggacgcc tttgtcgctc aggcactggc catgggcctg aaagggaaca aaacgctgcc
     4681 aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt gccattcagg acgaacgtga
     4741 agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag gctacggaaa ccaccttcac
     4801 ctggcagcca cagcgccgcc ggtaaggggt atttaccata acgtcgcatt gcccgcaccg
     4861 ctcagacggt ctgagggcgt ttccggcggc tacgggataa aacaggcaac aaaccacccg
     4921 gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct gcatgcctat gcacgcaact
     4981 gcatggcggg ccgtttggtg taaaaacgat acgataagcc gtgtttatgt aaatgaacgt
     5041 ggccgtaaag agtgacaaat atacatgtaa cattttttac tgacaggtca tcaacccgat
     5101 gcgtttaata ccctttatcc tgctggcatt gtgcagcccc ttactcctga cggcctgtaa
     5161 gcattccacc acccagccgc cgaaaattta tactctggat aagtcatcta aaatcagtag
     5221 ctacgctgcg gccatcaaga ccgctgttga aatgcagatg tttgaaccag aggcctatgc
     5281 gggaaaaatg tgcaccgtgc ggatatccat acaacgtgat ggcactctga acagcgccat
     5341 tgctgaagga ggagatccga aactctgtaa agcagcaata tcagccgtta cccacgccaa
     5401 aattccgccg gcaccggatg aaaatacctg gcaaatattc aaaaacgccc ctctggattt
     5461 cagaccatga aagcttttac tctgacagtg ctggcggcca tgttcctcac tccggtacat
     5521 gcgataccac taaaacccgt aaccacactg cagccaacct acccggtaca ggccgcagca
     5581 aaacgcatcg aaggtcaggt gaccgccacg tttgatattc tggacgatgg acgagtggac
     5641 aacgtgtcgc ttaccggctg tcaccttttt tttcgggaaa cgctgacggc aatgaggcac
     5701 tggctatatc ccgcagggca cccggcaatg cgacaggtta taaattttga attcagtctg
     5761 aaaaatgacg gcttacagga tccgcgacca gtcaccaccc aaaagctcaa tgatgatggt
     5821 tgccgccagt aacaggtcct ttaacccgca ggctgcaata gtcctggatg atgagtgagc
     5881 ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg ggcagcaaaa gccgtatttc
     5941 tggcaaatag cgccgccacg gattaaagta gtcatgtgta atacatatga ctacaaaggg
     6001 gcttttatgt catcagcaac atccatcagg ctcgatgaag aacttaagga ccggctgaaa
     6061 acgctggccg atgaccggca ccgctcagcg cacgcgctaa tgctggaggc aatcaccgaa
     6121 tacatcgacc gggaagagaa gcgcagccag tacctgcggg acggacaggc cgcatggcag
     6181 cactatcagg aaacgggtct gcaccttact gcggaagaag cggaagcctg gataagtacc
     6241 tggggaactg agaacgagca ggatgctccg ccatgccaca ggtaacgatt tccgcgctgg
     6301 cacagcgcga tttacaacgc cttcaggact ttctcaaaac caaaaatcgg ctggcagcca
     6361 gaaaaggagg tgaggtgatc gtccgggcta tccagcaact gaaaacattg ccagacattg
     6421 gccgcccggt gccgtttctg ccgctggaat ataaggaact ggtgatcggg tttggcgaca
     6481 gtggctatgt gatgctctac cgccacgacc gggaaatgga ccagattgtg atcgtcaccg
     6541 tcaggcatca gaaagaatcc gggtatccgg gggcagacag cctctaataa ccgatgacgg
     6601 gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc agaaaaccac
     6661 atcctgaacg gaaaaacggg taaaattcag cgatacattt accgtgagaa attacacacc
     6721 tttcacaccc gataaaatca ggggtataca gtggcgaaaa cattgctcaa cctttcacag
     6781 gcagcacaag ccgcgggcat taccagaaga acgctataca atcatgtaaa acagggaaag
     6841 gttaccgtgt cacgtgacgg gaaaaacaac cctgtcgtgg atgtgtcaga actgatccgt
     6901 gtatacggaa atgtgaatat ccctgagaaa caaattcccg gcatttcaca ccgtgaaaat
     6961 acacaaaaaa atttcccaca ggaacagctt ctggcaatgc aaaaggaact ggccgatctc
     7021 cggcaggctg tcaccctgat gctggaagat aaaacatccc gggaagaaga acgccgccag
     7081 cacgatgatg agcgccggaa actccaggcc gaagtggaca gattgacaac agaactgaca
     7141 cagaagaaaa aacgcttctg gtccggctgg tttagctgag aacttaccga agggaatgga
     7201 gaacatgacg aagcaggaaa aaactgccct caatatggct aaattcatta aggatcagtc
     7261 gctgctgttg ctggaaaagc tgaacgagct ggacatggat accccggccg acatgtgcga
     7321 acggcttcac gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcggggaaga
     7381 ataacagcac cggccatgcc ggtgctggcg ttattttttg ccgaacggcc accaccggcg
     7441 ggcgggtttg tcctccggca gttcaggcac cggcacgtta aaatagcggc tgttatattc
     7501 ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtgcc gctcgctgac
     7561 cacctcaatc atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa ccacctgcac
     7621 accgttctcg atggcgaacc gctccacctt tcgatagagg taatcacaca gcttcattcc
     7681 tgtgggtttg tcccgttccc attgcggatt ttcaccggtg aaaaagtacg tggctttttc
     7741 gtcgctgttg agccgctcgt catacatccc gatggatttc atcatcgcca gatgctgggc
     7801 ccggtcgaca aaacagaaaa catacgcccc ggtctgccag gcgagcacca ccatgtcttc
     7861 cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca cgtcctcaat
     7921 aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa agcacgcgaa
     7981 cagcctctcc cggtccagct ccaccgtttt gcgggcctcg ccggtctgtg gattcacgat
     8041 ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt cagggcagat
     8101 gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac agatacccgg acaccacttc
     8161 ctgataccgc ctgcggctct tgacggtgcg ctctacctca aatgccaccg ttctgccgtc
     8221 cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt gcaggtaccg
     8281 ccagccggtg gccccccggc cctccatgga gaggcgcagg gactgcacgg ccagccggtg
     8341 cggcagctgc gccgccgaca cccttgacgg ctgaaacgga ataacgtccg tcagcggctc
     8401 gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc tgacctgacg
     8461 gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccagac tgtccaggaa
     8521 gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct ccgccgtgga
     8581 acacagctct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt tccgcgcctg
     8641 ccgcttcgcg tggctgacgt tgtactgctc cagtgtcatc agtaaagccc cgcaaaatca
     8701 tcatccgctt ttggtccgcc gccgtcggcc ggaggcaggt actccgccag cgggtccgtt
     8761 ttcgccagca ccgggaaggt tttcagcgaa atttcccggc gctcaacccg cagcgggctg
     8821 ctgaacgcca gccgcgccac gcccagcccg gtcatcaccg cgaacccgtc aggcaggtgt
     8881 tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct ggatgaagct gtcgccgctg
     8941 atgtgctcca cgttccccgc ctcccggctg gcccggcggc gctccacgtc cacccggatc
     9001 tccccggtct ggcccgccgc ccactgcgcg gactcctggc tggccgcgcg atagaaccag
     9061 cggatcggcg tgttatccag taccgtggtt ttcacaaaat cagcgggcag atcctgcccg
     9121 cactgaccga agtcgcccag cgactggtgc gcgagcagca ggttgcagtc ccggctgcgc
     9181 agcgtcccca gcgcgttcag cacgtacttc gacagcagat atttaatctc atcgagcatg
     9241 atgctcgcgt gcggccacct ccggaactca tcccgcgcaa tgatgatttg cgcgcagcgg
     9301 gcaaacagca tcttctgcac ccggatcacc gcctcatcgt ccatcgagcc gatgacgtac
     9361 aggcagccgc cgccgttgat tatccccgcc acatcgatac cgctgtccgt ctgcaggacg
     9421 ctcaggtcgg ccacgttctc cagcttggtg atcagcccct tcacgtcctt tttcagggcc
     9481 tccggcaacg cgtacgccgc cgccagcacc tgctgaatgt ttgccccctg cgggaactgc
     9541 tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt agtggtccgc cacgtcgccg
     9601 gtctcactca ggttaaaccc ggcaaccagc agattcttca gggcgtactg gtcgagatcg
     9661 cggaacagat tcagctgcgc cggtttcccc ggccgcagat ccagcagggt gaagtccggg
     9721 cagtgcgccc ggaagacgtg cggcgcccac gcgtcccctt tggggtcgaa gtacaccaca
     9781 gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg tggccatcgc gctcttgccg
     9841 ctcccgggca ggccgaggat ctgaatattg gtcttgcgcc aggtatcacg agggaccagc
     9901 accggctggc cctgctcatc cttacccaga aacaccgcct gctgtttccg ggcctgctcc
     9961 gtggaaaaat cggccagcgc gttaaccggt accggctccc cgaaatgcag gtggcggacg
    10021 tctgtcattt ctccggtccg ctggctgctg cgccggctct
//
