LOCUS       NZ_CP028788            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP020030 plasmid p1_020030, complete
            sequence.
ACCESSION   NZ_CP028788
VERSION     NZ_CP028788.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08624619
            Assembly: GCF_003038215.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 10060)
  AUTHORS   Liu,L., Lin,J., Tang,G., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:45:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,921
            CDSs (total)                      :: 5,801
            Genes (coding)                    :: 5,636
            CDSs (with protein)               :: 5,636
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 60 of 165
            Pseudo Genes (incomplete)         :: 113 of 165
            Pseudo Genes (internal stop)      :: 21 of 165
            Pseudo Genes (multiple problems)  :: 28 of 165
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028788.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028788.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020030"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_020030"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2016-07-18"
     gene            join(9792..10060,1..262)
                     /locus_tag="C6M02_RS00005"
                     /old_locus_tag="C6M02_00010"
     CDS             join(9792..10060,1..262)
                     /locus_tag="C6M02_RS00005"
                     /old_locus_tag="C6M02_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(289..468)
                     /locus_tag="C6M02_RS00010"
                     /old_locus_tag="C6M02_00015"
     CDS             complement(289..468)
                     /locus_tag="C6M02_RS00010"
                     /old_locus_tag="C6M02_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(494..922)
                     /locus_tag="C6M02_RS00015"
                     /old_locus_tag="C6M02_00020"
     CDS             complement(494..922)
                     /locus_tag="C6M02_RS00015"
                     /old_locus_tag="C6M02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1086..1400)
                     /locus_tag="C6M02_RS00020"
                     /old_locus_tag="C6M02_00025"
     CDS             complement(1086..1400)
                     /locus_tag="C6M02_RS00020"
                     /old_locus_tag="C6M02_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1388..1666)
                     /locus_tag="C6M02_RS00025"
                     /old_locus_tag="C6M02_00030"
     CDS             complement(1388..1666)
                     /locus_tag="C6M02_RS00025"
                     /old_locus_tag="C6M02_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1841..2206)
                     /locus_tag="C6M02_RS29435"
                     /old_locus_tag="C6M02_00035"
     CDS             complement(1841..2206)
                     /locus_tag="C6M02_RS29435"
                     /old_locus_tag="C6M02_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2203..2574)
                     /gene="tolA"
                     /locus_tag="C6M02_RS00035"
                     /old_locus_tag="C6M02_00040"
     CDS             complement(2203..2574)
                     /gene="tolA"
                     /locus_tag="C6M02_RS00035"
                     /old_locus_tag="C6M02_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2848..3093)
                     /locus_tag="C6M02_RS00040"
                     /old_locus_tag="C6M02_00045"
     CDS             complement(2848..3093)
                     /locus_tag="C6M02_RS00040"
                     /old_locus_tag="C6M02_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3420..5105
                     /locus_tag="C6M02_RS00045"
                     /old_locus_tag="C6M02_00050"
     CDS             3420..5105
                     /locus_tag="C6M02_RS00045"
                     /old_locus_tag="C6M02_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5115..5372
                     /locus_tag="C6M02_RS00050"
                     /old_locus_tag="C6M02_00055"
     CDS             5115..5372
                     /locus_tag="C6M02_RS00050"
                     /old_locus_tag="C6M02_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5457..5606
                     /locus_tag="C6M02_RS00055"
                     /old_locus_tag="C6M02_00060"
     CDS             5457..5606
                     /locus_tag="C6M02_RS00055"
                     /old_locus_tag="C6M02_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7090..9054
                     /locus_tag="C6M02_RS00060"
                     /old_locus_tag="C6M02_00070"
     CDS             7090..9054
                     /locus_tag="C6M02_RS00060"
                     /old_locus_tag="C6M02_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9054..9785
                     /gene="mobC"
                     /locus_tag="C6M02_RS00065"
                     /old_locus_tag="C6M02_00005"
     CDS             9054..9785
                     /gene="mobC"
                     /locus_tag="C6M02_RS00065"
                     /old_locus_tag="C6M02_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5710..6288)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9055..9782
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gctgtgtgat tacctctatc gaaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
       61 ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg tggtcagcga
      121 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
      181 cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg
      241 gtggccgttc ggcaaaaaat aacgccagca ccggcatggc cggtgctgtt attcttcccc
      301 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgaagcc gttcgcacat
      361 gtcggccggg gtatccatgt ccagctcgtt cagcttttcc agcaacagca gcgactgatc
      421 cttaatgaat ttagccatat tgagggcagt tttttcctgc ttcgtcatgt tctccattcc
      481 cttcggtaag ttctcagcta aaccagccgg accagaagcg ttttttcttc tgtgtcagtt
      541 ctgttgtcaa tctgtccact tcggcctgga gtttccggcg ctcatcatcg tgctggcggc
      601 gttcttcttc ccgggatgtt ttatcttcca gcatcagggt gacagcctgc cggagatcgg
      661 ccagttcctt ttgcattgcc agaagctgtt cctgtgggaa atttttttgt gtattttcac
      721 ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat acacggatca
      781 gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta acctttccct
      841 gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct gcctgtgaaa
      901 ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga aaggtgtgta
      961 atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg atgtggtttt
     1021 ctgacgcgct gcgcttgtca aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc
     1081 ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct gacggtgacg
     1141 atcacaatct ggtccatttc ccggtcgtgg cggtagagca tcacatagcc actgtcgcca
     1201 aacccgatca ccagttcctt atattccagc ggcagaaacg gcaccgggcg gccaatgtct
     1261 ggcaatgttt tcagttgctg gatagcccgg acgatcacct cacctccttt tctggctgcc
     1321 agccgatttt tggttttgag aaagtcctga aggcgttgta aatcgcgctg tgccagcgcg
     1381 gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc caggtactta
     1441 tccaggcttc cgcttcttcc gcagtaaggt gcagacccgt ttcctgatag tgctgccatg
     1501 cggcctgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg tattcggtga
     1561 ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc gttttcagcc
     1621 ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa gcccctttgt
     1681 agtcatatgt attacacatg actactttaa tccgtggcgg cgctatttgc cagaaatacg
     1741 gcttttgctg cccatttttc agcttcgctt ccactaatca gggctgctac aggctcactc
     1801 atcatccagg actattgcag cctgcgggtt aaaggacctg ttactggcgg caaccatcat
     1861 cattgagctt ttgggtggtg actggtcgcg gatcctgtaa gccgtcattt ttcagactga
     1921 attcaaaatt tataacctgt cgcattgccg ggtgccctgc gggatatagc cagtgcctca
     1981 ttgccgtcag cgtttcccga aaaaaaaggt gacagccggt aagcgacacg ttgtccactc
     2041 gtccatcgtc cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt tttgctgcgg
     2101 cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc gcatgtaccg
     2161 gagtgaggaa catggccgcc agcactgtca gagtaaaagc tttcatggtc tgaaatccag
     2221 aggggcgttt ttgaatattt gccaggtatt ttcatccggt gccggcggaa ttttggcgtg
     2281 ggtaacggct gatattgctg ctttacagag tttcggatct cctccttcag caatggcgct
     2341 gttcagagtg ccatcacgtt gtatggatat ccgcacggtg cacatttttc ccgcataggc
     2401 ctctggttca aacatctgca tttcaacagc ggtcttgatg gccgcagcgt agctactgat
     2461 tttagatgac ttatccagag tataaatttt cggcggctgg gtggtggaat gcttacaggc
     2521 cgtcaggagt aaggggctgc acaatgccag caggataaag ggtattaaac gcatcgggtt
     2581 gatgacctgt cagtaaaaaa tgttacatgt atatttgtca ctctttacgg ccacgttcat
     2641 ttacataaac acggcttatc gtatcgtttt tacaccaaac ggcccgccat gcagttgcgt
     2701 gcataggcat gcagcgggtt acaatttacc gggcgcgttt ccggcggttt tccgggtggt
     2761 ttgttgcctg ttttatcccg tagccgccgg aaacgccctc agaccgtctg agcggtgcgg
     2821 gcaatgcgac gttatggtaa atacccctta ccggcggcgc tgtggctgcc aggtgaaggt
     2881 ggtttccgta gcctcgtatt tctcccgacg catttcctgc gcccgcctgg cttcacgttc
     2941 gtcctgaatg gcacgaagca ccctttcccg cacctgttgt tttaccgcct ttggcagcgt
     3001 tttgttccct ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg ccaggcgctt
     3061 ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc tccgggcgtc tcaggtttcc
     3121 cgaacagttt aaccgcaggt cctctcctca tgccagttag ctttcagatg tgtctatacc
     3181 atctgaaatg taagtgtgat gaatgtctta aataataaaa tttgttgcca tgagaaattt
     3241 tcgggaaaag acgcgatctg cttacaggtg gctccgggcg cttttttgtg cctgaagatg
     3301 agaactaatc tcatatattg acacctgaaa acgggaggag taaggtaata attatactgt
     3361 gtatatgtac agtatgttgg ctttaattta ttgttttaaa tgtcaaagag gaaaacggta
     3421 tgagtggtgg agacggtcga ggtccgggta attcaggtct gggacataat ggtggtcagg
     3481 ccagtgggaa tgtgaacggt acgtctggta aaggtggccc ttcatcaggt ggtggtacgg
     3541 atccaaacag cgggccgggc tggggtacga cgcatacgcc gaatggagat attcataact
     3601 ataatccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggt ggtaacagcg
     3661 gcaatcatag cggtagctct ggtggcggac agtcttcggc cactgcgatg gccttcggtc
     3721 tgcctgccct ggctactccg ggggctgaag gactggcttt atcagtttcc ggcgatgcgt
     3781 tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg gccgtttaag tttggtctgt
     3841 gggggattgc gatctacggt gtgctgcctt ctgagattgc aaaagatgat ccgaaaatga
     3901 tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac ggagactccg gcaagtactt
     3961 taccactgga ccaggcgacg gttcgtgtca gacaacgggt tgtggatgtg gtgaaggatg
     4021 agcggcagca tattgcggtt gtcgcaggtc ggccaatgag tgtccctgtg gtggatgcga
     4081 aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg tctctgcagg
     4141 tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc aaaaggcatt gttgctgaaa
     4201 aaggtgattc acgtccggct ggttttacag ccggtggtaa ctcccgtgag gccgttattc
     4261 gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat gttcttaccc
     4321 cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg ccagcaggca tgggacgccg
     4381 ctcatccgga agaggggctg aaaagagact atgataaagc gaaagctgag ctggatgccg
     4441 aagataaaaa tattgcgacc ttaaacagcc gcattgcatc gacagagaag gcgctccccg
     4501 gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag gcaaataaag
     4561 atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag gatcaggttc
     4621 gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcggct caggcatctt
     4681 taaacgcaat gaatgaatcc ttatccagag ataaggctgc acttaccggg gcgatggaga
     4741 gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taagttaaat gaggaaaaga
     4801 acaagcctcg caaggggact aaagactacg gccatgatta tcatccagta cctaaaacag
     4861 aagacataaa gggacttgga gagttaaagc gaggtgatcc taaaacacct aaacaaggtg
     4921 gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg taaaatttat gaatgggatt
     4981 cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg cgaacaccta ggcgcatttg
     5041 atcccaaaac gggtaagcag gttaaagggc cagatccaaa acggaacatt aaaaaatatc
     5101 tttgagaggt aaatatgggg cttaaattac gtttgacatg gtttgataag aaaactgaag
     5161 agtttaaagg ggaagagtat tctaaagact ttggtgacga tggttctgtc attgaaagct
     5221 tgggaatgcc tttaaaggat aatattaata atggtgagtt tgatttgaag aaagaatggg
     5281 ttcctttact acagccattt tttaaaaatc aaataaaacc tgatgtggat aattgttata
     5341 tagcttttga ttatcgggac ggtaactggt aacaaaaaat tatcaaggat gatttgaaat
     5401 actgacttcc ggtgttcagg gatgaatgcc ggagtcttta aatacaggga aagtatatga
     5461 aaaaggtaaa gactattttt ctttttatat tgattgttgc tggctttctt ctggccgcat
     5521 gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt ggcaccatcg tcctcctctg
     5581 aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt
     5641 tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga
     5701 aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa
     5761 ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt
     5821 ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt
     5881 gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc
     5941 tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga
     6001 taccagtgtg tgagctatga gaaagcgcca cacttcccgc aagggagaac ggcggacagg
     6061 tatccggtaa acggcagggt cggaacagga gagcgcaaga gggagcgacc taccggaaac
     6121 ggtggggatc tttaagtcct gtcgggtttc gcccgtactg tcagattcat ggttgagcct
     6181 cacggctccc acagatgcac cgaaaaagcg tctgtttatg tgaactctgg caggagggcg
     6241 gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat gttagtcccc
     6301 tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc
     6361 acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctgcaaga
     6421 cggtcaccgg acgcgctgat gtgggatatt gtcccctgat taagtctttc tattccctga
     6481 cgatcaatta tcaggatagg tgaaccatgt cggtttttcg ctgaacattt tacgttaacc
     6541 tatctctgtt gcttttttct ccttgcggag aaatgcaagt catccccgct ggggcgtggc
     6601 tgacttcagg ggaaaagacc ttgcttacgc tggctggacg ccaccgacgc acggtctttt
     6661 cccctctttc tggctctgtc cggctggacg ccggccacgc cagaaattca cccggccccc
     6721 gtcaggctca cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tgacgaagca
     6781 gcaaagctgc tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg
     6841 gccggaacac cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg
     6901 tgctcgttgt tcctccgggg ctgcgccttt tccgcatggg gcggaaagag gctggcctcc
     6961 gcggtgaaag ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc cttggggcat
     7021 gtccgcggct tgtgcagcgg gggctgcgca tcacgccggt aaacccgtcg tctgaaggag
     7081 agagattgca tgtttaatac ggattcgctt gcctggcagt ggcatatccc ggagctggcc
     7141 cggcagattt acccgtgggt cagctggctg taccgggagt attacagtct ggccggtttt
     7201 tttctggtta tcggcctgtt tttcggtctg gctgaactct gggccggccg aatgcgtttt
     7261 gtggggctga gccataaggc gcggcgggag tatgccgatc gctataagcg cgataataac
     7321 cggactgtgc cattttcggt gctggcctcg ctggattcac ccgggcggat ggtcagggca
     7381 cggcgacatg tctggaacta cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg
     7441 ggggggctgg ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgcccggac
     7501 ctctatgcga acgcagatta ttacctgctg cagccggcta acctgttcgg gatagcctgg
     7561 tgtgccggag ggcttgtcgc cggcgtcctt ctggcgcggc tgctggcgtt cctgctgctt
     7621 gatggtcatt ttgcggccgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg
     7681 cgcagcagcc agcggaccgg agaaatgaca gacgtccgcc acctgcattt cggggagccg
     7741 gtaccggtta acgcgctggc cgatttttcc acggagcagg cccggaaaca gcaggcggtg
     7801 tttctgggta aggatgagca gggccagccg gtgctggtcc ctcgtgatac ctggcgcaag
     7861 accaatattc agatcctcgg cctgcccggg agcggcaaga gcgcgatggc caccaacgcg
     7921 ctcattcgct gcgtacgcga tttcggggac gctgtggtgt acttcgaccc caaaggggac
     7981 gcgtgggcgc cgcacgtctt ccgggcgcac tgcccggact tcaccctgct ggatctgcgg
     8041 ccggggaaac cggcgcagct gaatctgttc cgcgatctcg accagtacgc cctgaagaat
     8101 ctgctggttg ccgggtttaa cctgagtgag accggcgacg tggcggacca ctaccgcatc
     8161 agcgagcaga aagcggcgaa gctgattgcg gagcagttcc cgcagggggc aaacattcag
     8221 caggtgctgg cggcggcgta cgcgttgccg gaggccctga aaaaggacgt gaaggggctg
     8281 atcaccaagc tggagaacgt ggccgacctg agcgtcctgc agacggacag cggtatcgat
     8341 gtggcgggga taatcaacgg cggcggctgc ctgtacgtca tcggctcgat ggacgatgag
     8401 gcggtgatcc gggtgcagaa gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg
     8461 gatgagttcc ggaggtggcc gcacgcgagc atcatgctcg atgagattaa atatctgctg
     8521 tcgaagtacg tgctgaacgc gctggggacg ctgcgcagcc gggactgcaa cctgctgctc
     8581 gcgcaccagt cgctgggcga cttcggtcag tgcgggcagg atctgcccgc tgattttgtg
     8641 aaaaccacgg tactggataa cacgccgatc cgctggttct atcgcgcggc cagccaggag
     8701 tccgcgcagt gggcggcggg ccagaccggg gagatccggg tggacgtgga gcgccgccgg
     8761 gccagccggg aggcggggaa cgtggagcac atcagcggcg acagcttcat ccagaaggag
     8821 gcgcggccgc tgtttgacgt gaacaccctg caacacctgc ctgacgggtt cgcggtgatg
     8881 accgggctgg gcgtggcgcg gctggcgttc agcagcccgc tgcgggttga gcgccgggaa
     8941 atttcgctga aaaccttccc ggtgctggcg aaaacggacc cgctggcgga gtacctgcct
     9001 ccggccgacg gcggcggacc aaaagcggat gatgattttg cggggcttta ctgatgacac
     9061 tggagcagta caacgtcagc cacgcgaagc ggcaggcgcg gaacgcggag aaaacacggc
     9121 tgaccctgcg ctggctgcgg gaagagctgt gttccacggc ggagctggtt gcgcgacggc
     9181 tggggattgc ggcggtacag ccggtttacc gcttcctgga cagtctggtg gcgaaagggc
     9241 tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt cagcgtgtgg gggctgacgc
     9301 cgcacggggt ggcgttctcg ttcgacgagg acgagccgct gacggacgtt attccgtttc
     9361 agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct ggccgtgcag tccctgcgcc
     9421 tctccatgga gggccggggg gccaccggct ggcggtacct gcaccgtatg gccctgaaag
     9481 ggatgaaagt gccggacgcg ctggcggagc tggacggcag aacggtggca tttgaggtag
     9541 agcgcaccgt caagagccgc aggcggtatc aggaagtggt gtccgggtat ctgttcaacc
     9601 gccgggcgaa cggcatcgat gaaatctggt acatctgccc tgaccggccg acgcttgtcc
     9661 gggtacagcg ggcgattctg gcggtggatg aaatcgtgaa tccacagacc ggcgaggccc
     9721 gcaaaacggt ggagctggac cgggagaggc tgttcgcgtg ctttaaattt atgacaacgg
     9781 agtgagggat tatggcgctg tacatggtat ttattgagga cgtggaagac aaagacggcg
     9841 agcgctgcat tatgccaacc agcgagcatc tggaagacat ggtggtgctc gcctggcaga
     9901 ccggggcgta tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca
     9961 tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcaccggtg
    10021 aaaatccgca atgggaacgg gacaaaccca caggaatgaa
//
