LOCUS       NZ_CP028696             6676 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain 107 plasmid pTA107-2, complete sequence.
ACCESSION   NZ_CP028696
VERSION     NZ_CP028696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08773019
            Assembly: GCF_005222445.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     WGA of Escherichia coli O157:H7: comparison of multiple isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-FEB-2017
            Assembly Method                   :: HGAP v. RS_HGAP_Assembly.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005222445.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:48:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,544
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,128
            CDSs (with protein)               :: 5,128
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 108 of 285
            Pseudo Genes (incomplete)         :: 163 of 285
            Pseudo Genes (internal stop)      :: 65 of 285
            Pseudo Genes (multiple problems)  :: 41 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028696.1.
            Bacteria available from Dr. Terry Arthur lab at the U.S Meat Animal
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="107"
                     /serotype="O157:H7"
                     /isolation_source="cattle"
                     /db_xref="taxon:562"
                     /plasmid="pTA107-2"
                     /collection_date="2003"
                     /collected_by="USMARC"
     gene            complement(985..1131)
                     /locus_tag="C9E36_RS27680"
                     /old_locus_tag="C9E36_28960"
     CDS             complement(985..1131)
                     /locus_tag="C9E36_RS27680"
                     /old_locus_tag="C9E36_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(1201..1464)
                     /locus_tag="C9E36_RS27685"
                     /old_locus_tag="C9E36_28965"
     CDS             complement(1201..1464)
                     /locus_tag="C9E36_RS27685"
                     /old_locus_tag="C9E36_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(1461..3554)
                     /gene="cda"
                     /locus_tag="C9E36_RS27690"
                     /old_locus_tag="C9E36_28970"
     CDS             complement(1461..3554)
                     /gene="cda"
                     /locus_tag="C9E36_RS27690"
                     /old_locus_tag="C9E36_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            3818..4063
                     /locus_tag="C9E36_RS27695"
                     /old_locus_tag="C9E36_28975"
     CDS             3818..4063
                     /locus_tag="C9E36_RS27695"
                     /old_locus_tag="C9E36_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(4553..4786)
                     /locus_tag="C9E36_RS27705"
     CDS             complement(4553..4786)
                     /locus_tag="C9E36_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4793..5353)
                     /locus_tag="C9E36_RS27710"
     CDS             complement(4793..5353)
                     /locus_tag="C9E36_RS27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5388..6068)
                     /locus_tag="C9E36_RS29205"
                     /pseudo=""
     CDS             complement(<5388..6068)
                     /locus_tag="C9E36_RS29205"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6031..6354)
                     /locus_tag="C9E36_RS27720"
                     /old_locus_tag="C9E36_28995"
     CDS             complement(6031..6354)
                     /locus_tag="C9E36_RS27720"
                     /old_locus_tag="C9E36_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6422..6613
                     /locus_tag="C9E36_RS27725"
                     /old_locus_tag="C9E36_29000"
     CDS             6422..6613
                     /locus_tag="C9E36_RS27725"
                     /old_locus_tag="C9E36_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..6676
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(23..106)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="1.15e-36"
     Typing          complement(569..756)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(4447..6041)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6414..6616)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.53e-12"
ORIGIN
        1 cgtcagggcg cgtcagcggg ttttagcggg tgtcggggcg cagccatgac ccagtcacgt
       61 agcgatagcg gagtgtatac tggcttaatc atgtggcatc agtgaggatg gtatgaaaag
      121 tgcaccatgc cgggtgtgaa atgccgcaca gatgcgtaag gagaaaatgc tcgtccaggc
      181 gcttttccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg
      241 actctccttt ctcaaaggcg gtaaccccgt tatccacaga atccggggat aacccgggaa
      301 agaacatgtg cgcaaaaaaa cgaagacccc gggaaaggcc gcggcacagg cgctttttcc
      361 ataggctccg cccccctgac ggacttcaca aaacagacac tttttcggtg catctgtggg
      421 agccgtgagg ctcaaccatg aaatccgcca gcggtggcga aacccgacgt ggcttaaaga
      481 tcatcaccgt ttccggtgaa tcgctccctc ctgcgccccc acgttcccgc catatcgatt
      541 accggatacc ttttccgcct ttctcccttg cgggacacgt ggcgctttct catagctcac
      601 gtcttctggt atctcggggc gacttaggca gttcgctccg ggctgggctg tatgcaggaa
      661 ccccccgttc agcccgactg ctgctccttc tccggtaact gttctcttgg ggccaacccg
      721 gaaaagacac gacttaacgc cactggcagc agccattggt aactgattta gaggagttgc
      781 tcttgaagtt atgaaccagt taaggctaaa ctgaaaagac agttttggtg actgtgctcc
      841 tccaagccag ttaccaccgc cgattagaag aggtcgaaga cttctgatcg gacggaagga
      901 cccgccacaa ggcgggtttt ttcgtttaca ggcagcagat tacgcgcaga aaaaaaggat
      961 ctcaagaaga tcctttaata tttttcactg aaccgccacc ccgattagtt tcgaagatga
     1021 tgatggagca attgttccac cctgaacatc ccttacatag ttagcttggc aagcagtcag
     1081 acacaaaaca gcaagaacga gaatcataga tccccctatc ttttttttca tgacatctcc
     1141 ttgtcatgag tgaatatctg acatttatac ttaaatgcca gatattaaaa tcactcatga
     1201 ttataattta aatttttcca agatagatcg gacctcaacc ttaagtccat tatcatcaat
     1261 ttcataatca gccctatctg catcaggctc aaatcgctca gcagccataa acaactcttc
     1321 cccacaattt aatatattcg gagacaaatc cttaacacca tacaatcttc tccgttcaac
     1381 acaaattcgc tctgaaaact caattgctgt aattttcgaa gctaaaaata atttcgccaa
     1441 atcgatcatt gccatcttgt tcatagttca cctgtctcta aataaattcg accattatcc
     1501 gcatctggat ttattttcca cccagataag aactcaccat ttgacttaat tataaccaca
     1561 ttcatcgtat taggattaaa ataaactttt gaaccttttt ctcttcggta tgttcctttc
     1621 tctactgtat ccttatccga taaatgctcc tcaatagcat ccctgaattt agtaagagtt
     1681 tcacgattct tttttgtatc actaatacca aaatcaccag catgtttata ttttttatca
     1741 agttgtttac gcgaaaatcg ccctgaatcc agaggatcat taagcatcac ataaacagct
     1801 tcaacaccag tcccagcagc atcaggacgc cagtaaataa aatctcgtag tccaccaaca
     1861 tcagaaacag gagtaatcgt cgttactggc atttcgaccg gttttacttc cgttcctgta
     1921 tgcactggtg taaccggaac gggttgatga ttacctgtat ctgttggcac cgaaggttgt
     1981 ggtaccggat taaccagaat cgtccgggaa ggtgcgccta ctactgctgg taacgtgatt
     2041 ttatccagtc ctgttgctgt atcacggaca gcattaagaa caggaaccgc agctgggata
     2101 ctttcattcc ctgttttcag taaatccaga gctaattgcc cgttactgtt tatcaattgt
     2161 ccacgaacag gcagatttac actcgtcgca ccaggttcaa tgaccacgcc ctgtccagcc
     2221 aacatctgag cactcagcga aaacatcgcc tccagatttc gccccggcac gatatcactt
     2281 cctgctccag cttcttctga atgaaaccac agagcaccaa gagaaatccg cataagcagg
     2341 ggactcactt cgggtatggt tactttctcc agagcattac tgaaaaactt gcttatgttg
     2401 cctgcaatat caccagcagc agtccagaca ccagttccgg tcatcgccag ttgtacagaa
     2461 ctggcagtgt tattcagaac aagagctccc tgctcattca ttactgaatt agccctttcc
     2521 atcatttcct gtgtatatac aggaggctgg ttcacacctg ctttactaaa taactcggct
     2581 tttgcttttt cagcttcagc ccgggctacc gcttcttctt tcttgactct gaaatcatcc
     2641 tttgctttat tttcggcttc ctgacgctga ctcagaccat tactgtccag aatctctaca
     2701 gttgaaatat ataacggaga aacaccactg ccatcaggaa aacgaacaat gacatcatga
     2761 ttattactgg acatcgtata accagcctca acataattac ctgtctcagc aataactcct
     2821 tcttcctgaa tttcgggatt agacagaacc ggagtattca tctgacccac acttaatacc
     2881 aggtccggta gatttggtga tacaggggca ctgtaaacat ctttaatatt tgttcccgga
     2941 cgactccggt ccggaactgg aacggcatca actaccggca ctgtcatcgg aatacctccg
     3001 ataatagcca tatattgcgt accgttctgc ataaggtcca tcagacgata tttcaccggt
     3061 acattattac ccgctgctgg taactggctg gccggaatat ccgttacctg ttccagttgc
     3121 tgtgtaacca taagacgaat aattttttgc tcatttccgg aagcaggctc cagacgggca
     3181 aaagaaacag cagccgggcg agacggttct ggcaaaccac cacgtaaacc attctcaaga
     3241 actgttcgtg cagcggaaac tttctcgttt ccccaagggc tatgcacact gacagaccat
     3301 cccgaccagt ctccctcttc aagttcctta tccagaataa tccggcgaat aattcccgga
     3361 aaaagaacac tttgtacaag tgcgataccc tgtgggctgt aatcccccca tcccggaggc
     3421 atggatggag cgatacctga tgagcctcct ggtttcacat caccacccga aatcagtcct
     3481 gttgacggcc acacgaccat cgagtgccca taatcaatac cttctgtcgg accactacct
     3541 tcgtaatcac tcataaaaac acctctttga cttttaaaac aatgcgttaa aaacaacaaa
     3601 ctgtacataa aaccactggt tttatgtaca gtaaaaagtt acgtcacctc gtaaatcctg
     3661 tcaacccact ggatttacgt ttttgtgaaa acgctcaaga aaaacaacca atcattctta
     3721 aactcaatgg caacaggcat gaaagaaggc tgattagcat aaggagagag ccaacagtta
     3781 aactgttatg acaccagaga aacacccgga gaccaccatg aaacgccata gactgaccga
     3841 agccgaagag cgccgactgg cagaagccat tgccgctgaa gctcgtgcca tggggctgac
     3901 gaaaaaggac aaaatgccgc aggaaacaaa gcgacgcctc cgggcacgga tacttaaagc
     3961 cattcttgat gaacgaacgg ccagcaaaaa tcgagaagcc acactgactg ccggacagga
     4021 cccggatgac accttcacct ggcaaccact gcgccgccgc taagctaagg gttatttaac
     4081 ataacccctt attgtacgca ccgctgaaat gcgttcagcg cgatcacggc tgctgacagg
     4141 taaaaatggc aacaaaccac ccgaaaagct gccgcgatcg cacctgataa attttaaccg
     4201 tatgcatagc tattcagcca tgtgaataac gctggttttg cctgcgtaaa cctcatgaca
     4261 ctgttttttt tccatctttt cagttgatga catacgcaga catcgcggga tgaggctgag
     4321 gaatgagcgc gatctggcaa agaggcaaaa cacagcaaca aaaacgacac gccagaatcg
     4381 cgcccggatg cgtttttaac gcgttccggt accatctggc aacctcccgg aacaactcac
     4441 cgtcacatcc ccattgacgg gccacgccat acccgtactt cccgttcctg ctcttcatgc
     4501 caggaccgcg cacgctcccg ttccaggcgt gccagccttt cctgttcatc ccttatctgc
     4561 tgttcgtgat aaataaccga ctcaagcggt ccacctgccc ggctaatctc tgcacctgct
     4621 gactcaagcc gtcgcactgt tccctcagtt gcccgttctc ctgtcgtgtc agctcgaaca
     4681 tatgctgcaa atccgtgaag gcgctctccc agtctttcag ccgctgcgta tagtcctgct
     4741 gcaattgctc taaggcgttc agtaagtgca tttccagctc tgtcattctc acttactccc
     4801 tgaccagtct tactgcgttc ttcttctcca ccgtccagtt gttttccccc ttcaccccgg
     4861 acggcaacac cagaaatttc ccgttcctgc cctcgtgata cgtcacaccc catgtttttt
     4921 cccggagttt cgccagcgtc tcttcctggt ccctgatagc caggatgttc gccgcaatac
     4981 ggctttcctg ccactgaatc agcccccatg acgccagaaa aatcccgccc gtgacgcaaa
     5041 gcgccgtcag cgacgggtac agtatccgcc ctttgaccag cttccagagc agctcttcct
     5101 gccgccgggc cagttcgttc tctgtggcgc tgaactgcgc gttcacggca ctgttcagcg
     5161 tctccagttg ttctttcacc gctgctgtgt gtgcgctgat agcgtctctg attttctgcc
     5221 cgtttaagtt cagttccctg tctacagacg cttcgagctt cctgaactcg ctgttcagca
     5281 tgttctctgt agagacggca cgctctttca gtttcttctc gaagtctgtc cccatttgta
     5341 aaagattgct catacagcgc ccctttcagc ctgagattac gcccgccctc cgggtcggcg
     5401 atactgatac tgctcctggt tgtcctcacc acctcaaaac ctgccgctgt aagcgcctca
     5461 gtgacatcct gacgcgtttt tagcgctccg gcatggtaaa gagcctccag tcccctcgta
     5521 atcgcttctg cggcctcctg tttcgctttc ggcagattat tcggggtgac aagtgtccgc
     5581 ctgttctccg gtgcgttcgg gtcgtgcagc ccgtaatggt gattcaccag tgtctgccag
     5641 gcattgattc gcggacggtc cgcccggtcg taatagggct ggagccgttt tccgctcgcc
     5701 agctccatat tcgggatgac aaaattcagc tcaagacgcc ccttgtcctg gtgctccacc
     5761 cacaggatgc tgtactgatt tttttcaaga ccgggcatca gtacccgctc aaagctctcc
     5821 atcacccttt cacgctctcc cggtggcagt gtctgctctg caaaagacag aacccccgag
     5881 gtgtattttt tcgcaaacgg cgtggcatcg atgagttccc gcacctcttc gggagcaccc
     5941 cgcagaactc tcgccccttc ccggttacgc tcccggccca gcaggtaatc aaccggacca
     6001 ctgccaccgc cttttcccct ggcatgaaac ttaactatca tcgcgttctc cctgtttacg
     6061 gacctcatcc ctcagctcac tcagttcacg tccgatggcc atcagtgcag ccaccacatg
     6121 aacccggtca tgccccgacc actgtccact gtttatcttc cgggctatct gattcaggtt
     6181 attgccgacc gaagcgaact ggcgcaacag cggcggtgcc agtgtcggaa gacctgacgt
     6241 tttcgatggc ggtgccccca ggcagacctt acgcatccat gacgcaagtt gttttccctc
     6301 acaacgtgcc agcagccgcg catgttcctc atccgtgacc cgtatcgtga gcatcctttc
     6361 gcgtttcacc ggtatcatta aaaacctccg acagactccc cacacatgga gaaacagaac
     6421 tgtgactaaa caggaaaaaa ccgcccttaa catggcccgc ttcatcagaa gccagacgct
     6481 gaccctgctg gaaaaactga atgaactgga cgccgacgac caggctgaca tctgcgaagc
     6541 gcttcacgac cacgctgacg agctttaccg cagctgcctc gcacgcttcg gggatgacgg
     6601 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgcctgt gagcggatgc
     6661 cgggagcaga caagcc
//
