LOCUS       NZ_CP028688             6676 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain 110 plasmid pTA110-2, complete sequence.
ACCESSION   NZ_CP028688
VERSION     NZ_CP028688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08773022
            Assembly: GCF_005222425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     WGA of Escherichia coli O157:H7: comparison of multiple isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-MAR-2017
            Assembly Method                   :: HGAP v. RS_HGAP_Assembly.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 193x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005222425.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:50:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,543
            CDSs (total)                      :: 5,412
            Genes (coding)                    :: 5,126
            CDSs (with protein)               :: 5,126
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 108 of 286
            Pseudo Genes (incomplete)         :: 164 of 286
            Pseudo Genes (internal stop)      :: 65 of 286
            Pseudo Genes (multiple problems)  :: 41 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028688.1.
            Bacteria available from Dr. Terry Arthur lab at the U.S Meat Animal
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="110"
                     /serotype="O157:H7"
                     /isolation_source="cattle"
                     /db_xref="taxon:562"
                     /plasmid="pTA110-2"
                     /collection_date="2003"
                     /collected_by="USMARC"
     gene            complement(join(5452..6676,1..869))
                     /gene="cda"
                     /locus_tag="C9E39_RS27665"
                     /old_locus_tag="C9E39_28935"
     CDS             complement(join(5452..6676,1..869))
                     /gene="cda"
                     /locus_tag="C9E39_RS27665"
                     /old_locus_tag="C9E39_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            1133..1378
                     /locus_tag="C9E39_RS27670"
                     /old_locus_tag="C9E39_28940"
     CDS             1133..1378
                     /locus_tag="C9E39_RS27670"
                     /old_locus_tag="C9E39_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(1868..2101)
                     /locus_tag="C9E39_RS27680"
     CDS             complement(1868..2101)
                     /locus_tag="C9E39_RS27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2108..2668)
                     /locus_tag="C9E39_RS27685"
     CDS             complement(2108..2668)
                     /locus_tag="C9E39_RS27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2703..3383)
                     /locus_tag="C9E39_RS29215"
                     /pseudo=""
     CDS             complement(<2703..3383)
                     /locus_tag="C9E39_RS29215"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3346..3669)
                     /locus_tag="C9E39_RS27695"
                     /old_locus_tag="C9E39_28960"
     CDS             complement(3346..3669)
                     /locus_tag="C9E39_RS27695"
                     /old_locus_tag="C9E39_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3737..3928
                     /locus_tag="C9E39_RS27700"
                     /old_locus_tag="C9E39_28965"
     CDS             3737..3928
                     /locus_tag="C9E39_RS27700"
                     /old_locus_tag="C9E39_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4976..5122)
                     /locus_tag="C9E39_RS27720"
                     /old_locus_tag="C9E39_28985"
     CDS             complement(4976..5122)
                     /locus_tag="C9E39_RS27720"
                     /old_locus_tag="C9E39_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(5192..5455)
                     /locus_tag="C9E39_RS27725"
                     /old_locus_tag="C9E39_28990"
     CDS             complement(5192..5455)
                     /locus_tag="C9E39_RS27725"
                     /old_locus_tag="C9E39_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     BGC             1..5869
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4014..4097)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="1.15e-36"
     Typing          complement(4560..4747)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(1762..3356)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3729..3931)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.85e-12"
ORIGIN
        1 tccagaatct ctacagttga aatatataac ggagaaacac cactgccatc aggaaaacga
       61 acaatgacat catgattatt actggacatc gtataaccag cctcaacata attacctgtc
      121 tcagcaataa ctccttcttc ctgaatttcg ggattagaca gaaccggagt attcatctga
      181 cccacactta ataccaggtc cggtagattt ggtgatacag gggcactgta aacatcttta
      241 atatttgttc ccggacgact ccggtccgga actggaacgg catcaactac cggcactgtc
      301 atcggaatac ctccgataat agccatatat tgcgtaccgt tctgcataag gtccatcaga
      361 cgatatttca ccggtacatt attacccgct gctggtaact ggctggccgg aatatccgtt
      421 acctgttcca gttgctgtgt aaccataaga cgaataattt tttgctcatt tccggaagca
      481 ggctccagac gggcaaaaga aacagcagcc gggcgagacg gttctggcaa accaccacgt
      541 aaaccattct caagaactgt tcgtgcagcg gaaactttct cgtttcccca agggctatgc
      601 acactgacag accatcccga ccagtctccc tcttcaagtt ccttatccag aataatccgg
      661 cgaataattc ccggaaaaag aacactttgt acaagtgcga taccctgtgg gctgtaatcc
      721 ccccatcccg gaggcatgga tggagcgata cctgatgagc ctcctggttt cacatcacca
      781 cccgaaatca gtcctgttga cggccacacg accatcgagt gcccataatc aataccttct
      841 gtcggaccac taccttcgta atcactcata aaaacacctc tttgactttt aaaacaatgc
      901 gttaaaaaca acaaactgta cataaaacca ctggttttat gtacagtaaa aagttacgtc
      961 acctcgtaaa tcctgtcaac ccactggatt tacgtttttg tgaaaacgct caagaaaaac
     1021 aaccaatcat tcttaaactc aatggcaaca ggcatgaaag aaggctgatt agcataagga
     1081 gagagccaac agttaaactg ttatgacacc agagaaacac ccggagacca ccatgaaacg
     1141 ccatagactg accgaagccg aagagcgccg actggcagaa gccattgccg ctgaagctcg
     1201 tgccatgggg ctgacgaaaa aggacaaaat gccgcaggaa acaaagcgac gcctccgggc
     1261 acggatactt aaagccattc ttgatgaacg aacggccagc aaaaatcgag aagccacact
     1321 gactgccgga caggacccgg atgacacctt cacctggcaa ccactgcgcc gccgctaagc
     1381 taagggttat ttaacataac cccttattgt acgcaccgct gaaatgcgtt cagcgcgatc
     1441 acggctgctg acaggtaaaa atggcaacaa accacccgaa aagctgccgc gatcgcacct
     1501 gataaatttt aaccgtatgc atagctattc agccatgtga ataacgctgg ttttgcctgc
     1561 gtaaacctca tgacactgtt ttttttccat cttttcagtt gatgacatac gcagacatcg
     1621 cgggatgagg ctgaggaatg agcgcgatct ggcaaagagg caaaacacag caacaaaaac
     1681 gacacgccag aatcgcgccc ggatgcgttt ttaacgcgtt ccggtaccat ctggcaacct
     1741 cccggaacaa ctcaccgtca catccccatt gacgggccac gccatacccg tacttcccgt
     1801 tcctgctctt catgccagga ccgcgcacgc tcccgttcca ggcgtgccag cctttcctgt
     1861 tcatccctta tctgctgttc gtgataaata accgactcaa gcggtccacc tgcccggcta
     1921 atctctgcac ctgctgactc aagccgtcgc actgttccct cagttgcccg ttctcctgtc
     1981 gtgtcagctc gaacatatgc tgcaaatccg tgaaggcgct ctcccagtct ttcagccgct
     2041 gcgtatagtc ctgctgcaat tgctctaagg cgttcagtaa gtgcatttcc agctctgtca
     2101 ttctcactta ctccctgacc agtcttactg cgttcttctt ctccaccgtc cagttgtttt
     2161 cccccttcac cccggacggc aacaccagaa atttcccgtt cctgccctcg tgatacgtca
     2221 caccccatgt tttttcccgg agtttcgcca gcgtctcttc ctggtccctg atagccagga
     2281 tgttcgccgc aatacggctt tcctgccact gaatcagccc ccatgacgcc agaaaaatcc
     2341 cgcccgtgac gcaaagcgcc gtcagcgacg ggtacagtat ccgccctttg accagcttcc
     2401 agagcagctc ttcctgccgc cgggccagtt cgttctctgt ggcgctgaac tgcgcgttca
     2461 cggcactgtt cagcgtctcc agttgttctt tcaccgctgc tgtgtgtgcg ctgatagcgt
     2521 ctctgatttt ctgcccgttt aagttcagtt ccctgtctac agacgcttcg agcttcctga
     2581 actcgctgtt cagcatgttc tctgtagaga cggcacgctc tttcagtttc ttctcgaagt
     2641 ctgtccccat ttgtaaaaga ttgctcatac agcgcccctt tcagcctgag attacgcccg
     2701 ccctccgggt cggcgatact gatactgctc ctggttgtcc tcaccacctc aaaacctgcc
     2761 gctgtaagcg cctcagtgac atcctgacgc gtttttagcg ctccggcatg gtaaagagcc
     2821 tccagtcccc tcgtaatcgc ttctgcggcc tcctgtttcg ctttcggcag attattcggg
     2881 gtgacaagtg tccgcctgtt ctccggtgcg ttcgggtcgt gcagcccgta atggtgattc
     2941 accagtgtct gccaggcatt gattcgcgga cggtccgccc ggtcgtaata gggctggagc
     3001 cgttttccgc tcgccagctc catattcggg atgacaaaat tcagctcaag acgccccttg
     3061 tcctggtgct ccacccacag gatgctgtac tgattttttt caagaccggg catcagtacc
     3121 cgctcaaagc tctccatcac cctttcacgc tctcccggtg gcagtgtctg ctctgcaaaa
     3181 gacagaaccc ccgaggtgta ttttttcgca aacggcgtgg catcgatgag ttcccgcacc
     3241 tcttcgggag caccccgcag aactctcgcc ccttcccggt tacgctcccg gcccagcagg
     3301 taatcaaccg gaccactgcc accgcctttt cccctggcat gaaacttaac tatcatcgcg
     3361 ttctccctgt ttacggacct catccctcag ctcactcagt tcacgtccga tggccatcag
     3421 tgcagccacc acatgaaccc ggtcatgccc cgaccactgt ccactgttta tcttccgggc
     3481 tatctgattc aggttattgc cgaccgaagc gaactggcgc aacagcggcg gtgccagtgt
     3541 cggaagacct gacgttttcg atggcggtgc ccccaggcag accttacgca tccatgacgc
     3601 aagttgtttt ccctcacaac gtgccagcag ccgcgcatgt tcctcatccg tgacccgtat
     3661 cgtgagcatc ctttcgcgtt tcaccggtat cattaaaaac ctccgacaga ctccccacac
     3721 atggagaaac agaactgtga ctaaacagga aaaaaccgcc cttaacatgg cccgcttcat
     3781 cagaagccag acgctgaccc tgctggaaaa actgaatgaa ctggacgccg acgaccaggc
     3841 tgacatctgc gaagcgcttc acgaccacgc tgacgagctt taccgcagct gcctcgcacg
     3901 cttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3961 cctgtgagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gttttagcgg
     4021 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaat
     4081 catgtggcat cagtgaggat ggtatgaaaa gtgcaccatg ccgggtgtga aatgccgcac
     4141 agatgcgtaa ggagaaaatg ctcgtccagg cgcttttccg cttcctcgct cactgactcg
     4201 ctacgctcgg tcgttcggct gcggcgagcg gactctcctt tctcaaaggc ggtaaccccg
     4261 ttatccacag aatccgggga taacccggga aagaacatgt gcgcaaaaaa acgaagaccc
     4321 cgggaaaggc cgcggcacag gcgctttttc cataggctcc gcccccctga cggacttcac
     4381 aaaacagaca ctttttcggt gcatctgtgg gagccgtgag gctcaaccat gaaatccgcc
     4441 agcggtggcg aaacccgacg tggcttaaag atcatcaccg tttccggtga atcgctccct
     4501 cctgcgcccc cacgttcccg ccatatcgat taccggatac cttttccgcc tttctccctt
     4561 gcgggacacg tggcgctttc tcatagctca cgtcttctgg tatctcgggg cgacttaggc
     4621 agttcgctcc gggctgggct gtatgcagga accccccgtt cagcccgact gctgctcctt
     4681 ctccggtaac tgttctcttg gggccaaccc ggaaaagaca cgacttaacg ccactggcag
     4741 cagccattgg taactgattt agaggagttg ctcttgaagt tatgaaccag ttaaggctaa
     4801 actgaaaaga cagttttggt gactgtgctc ctccaagcca gttaccaccg ccgattagaa
     4861 gaggtcgaag acttctgatc ggacggaagg acccgccaca aggcgggttt tttcgtttac
     4921 aggcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttaat atttttcact
     4981 gaaccgccac cccgattagt ttcgaagatg atgatggagc aattgttcca ccctgaacat
     5041 cccttacata gttagcttgg caagcagtca gacacaaaac agcaagaacg agaatcatag
     5101 atccccctat cttttttttc atgacatctc cttgtcatga gtgaatatct gacatttata
     5161 cttaaatgcc agatattaaa atcactcatg attataattt aaatttttcc aagatagatc
     5221 ggacctcaac cttaagtcca ttatcatcaa tttcataatc agccctatct gcatcaggct
     5281 caaatcgctc agcagccata aacaactctt ccccacaatt taatatattc ggagacaaat
     5341 ccttaacacc atacaatctt ctccgttcaa cacaaattcg ctctgaaaac tcaattgctg
     5401 taattttcga agctaaaaat aatttcgcca aatcgatcat tgccatcttg ttcatagttc
     5461 acctgtctct aaataaattc gaccattatc cgcatctgga tttattttcc acccagataa
     5521 gaactcacca tttgacttaa ttataaccac attcatcgta ttaggattaa aataaacttt
     5581 tgaacctttt tctcttcggt atgttccttt ctctactgta tccttatccg ataaatgctc
     5641 ctcaatagca tccctgaatt tagtaagagt ttcacgattc ttttttgtat cactaatacc
     5701 aaaatcacca gcatgtttat attttttatc aagttgttta cgcgaaaatc gccctgaatc
     5761 cagaggatca ttaagcatca cataaacagc ttcaacacca gtcccagcag catcaggacg
     5821 ccagtaaata aaatctcgta gtccaccaac atcagaaaca ggagtaatcg tcgttactgg
     5881 catttcgacc ggttttactt ccgttcctgt atgcactggt gtaaccggaa cgggttgatg
     5941 attacctgta tctgttggca ccgaaggttg tggtaccgga ttaaccagaa tcgtccggga
     6001 aggtgcgcct actactgctg gtaacgtgat tttatccagt cctgttgctg tatcacggac
     6061 agcattaaga acaggaaccg cagctgggat actttcattc cctgttttca gtaaatccag
     6121 agctaattgc ccgttactgt ttatcaattg tccacgaaca ggcagattta cactcgtcgc
     6181 accaggttca atgaccacgc cctgtccagc caacatctga gcactcagcg aaaacatcgc
     6241 ctccagattt cgccccggca cgatatcact tcctgctcca gcttcttctg aatgaaacca
     6301 cagagcacca agagaaatcc gcataagcag gggactcact tcgggtatgg ttactttctc
     6361 cagagcatta ctgaaaaact tgcttatgtt gcctgcaata tcaccagcag cagtccagac
     6421 accagttccg gtcatcgcca gttgtacaga actggcagtg ttattcagaa caagagctcc
     6481 ctgctcattc attactgaat tagccctttc catcatttcc tgtgtatata caggaggctg
     6541 gttcacacct gctttactaa ataactcggc ttttgctttt tcagcttcag cccgggctac
     6601 cgcttcttct ttcttgactc tgaaatcatc ctttgcttta ttttcggctt cctgacgctg
     6661 actcagacca ttactg
//
