LOCUS       NZ_CP028681             6078 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain 113 plasmid pTA113-2, complete sequence.
ACCESSION   NZ_CP028681
VERSION     NZ_CP028681.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08773025
            Assembly: GCF_005222385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     WGA of Escherichia coli O157:H7: comparison of multiple isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-MAY-2017
            Assembly Method                   :: HGAP v. RS_HGAP_Assembly.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005222385.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:55:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,572
            CDSs (total)                      :: 5,439
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 108 of 286
            Pseudo Genes (incomplete)         :: 162 of 286
            Pseudo Genes (internal stop)      :: 70 of 286
            Pseudo Genes (multiple problems)  :: 44 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028681.1.
            Bacteria available from Dr. Terry Arthur lab at the U.S Meat Animal
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="113"
                     /serotype="O157:H7"
                     /isolation_source="cattle"
                     /db_xref="taxon:562"
                     /plasmid="pTA113-2"
                     /collection_date="2006"
                     /collected_by="USMARC"
     gene            complement(join(5947..6078,1..102))
                     /locus_tag="C9E42_RS29000"
     CDS             complement(join(5947..6078,1..102))
                     /locus_tag="C9E42_RS29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029401705.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(109..627)
                     /locus_tag="C9E42_RS29010"
     CDS             complement(109..627)
                     /locus_tag="C9E42_RS29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_043991090.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<665..1315)
                     /locus_tag="C9E42_RS29015"
                     /pseudo=""
     CDS             complement(<665..1315)
                     /locus_tag="C9E42_RS29015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1305..1628)
                     /locus_tag="C9E42_RS27745"
                     /old_locus_tag="C9E42_29040"
     CDS             complement(1305..1628)
                     /locus_tag="C9E42_RS27745"
                     /old_locus_tag="C9E42_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001508824.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1693..1884
                     /locus_tag="C9E42_RS27750"
                     /old_locus_tag="C9E42_29045"
     CDS             1693..1884
                     /locus_tag="C9E42_RS27750"
                     /old_locus_tag="C9E42_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2525..2650
                     /locus_tag="C9E42_RS29320"
                     /old_locus_tag="C9E42_29060"
     CDS             2525..2650
                     /locus_tag="C9E42_RS29320"
                     /old_locus_tag="C9E42_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071535512.1"
                     /translation="MHEPPVQSDCCALSGNYPLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     gene            2959..3150
                     /locus_tag="C9E42_RS27775"
     CDS             2959..3150
                     /locus_tag="C9E42_RS27775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137049521.1"
                     /translation="MLPLSAARNLGDLLPLSAARNLGDLLPLSAARNLGDLLPLSAARN
                     SGDLLPLSAARNLGHLLP"
                     /name="hypothetical protein"
     gene            3204..3719
                     /locus_tag="C9E42_RS27780"
                     /old_locus_tag="C9E42_29070"
     CDS             3204..3719
                     /locus_tag="C9E42_RS27780"
                     /old_locus_tag="C9E42_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_000675353.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(3831..4541)
                     /locus_tag="C9E42_RS27785"
                     /old_locus_tag="C9E42_29075"
     CDS             complement(3831..4541)
                     /locus_tag="C9E42_RS27785"
                     /old_locus_tag="C9E42_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177388.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            5086..5343
                     /locus_tag="C9E42_RS27790"
                     /old_locus_tag="C9E42_29080"
     CDS             5086..5343
                     /locus_tag="C9E42_RS27790"
                     /old_locus_tag="C9E42_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001508827.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            5353..5634
                     /locus_tag="C9E42_RS27795"
                     /old_locus_tag="C9E42_29085"
     CDS             5353..5634
                     /locus_tag="C9E42_RS27795"
                     /old_locus_tag="C9E42_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177387.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     Typing          complement(1963..2047)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          2347..2864
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1315)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.836"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcaattcc acgaaggcgc tctcccattc gctcagccgc tgcatatagt cctgttgcag
       61 ctgctctaag gcgttcagca aatgtgtttc cagctctgtc actctgtatc actccttcag
      121 atgtacccat tccttcccct gaaaggaaat cacctctgct gacttcccgt aaggaaggac
      181 cagaaatttc ctgttcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt
      241 ctgtaatgtc tcttcctgct tcctgatatc ttccaggttc gcctgtatcc tcccgccaag
      301 ataccagagc gtcccgccac tcgcggtaaa caggagaaag actatcccca gtaacatcat
      361 gcccgtattc cctgccagct ttaacacgtc cctcctgtgc tgcatcatcg cctctttcac
      421 cccttcccgg tgtttttcca gcgattcctc cgtcgaggcc gtaaaccggg ctatagcgcc
      481 tttgattttc gtctcgtttg aggtcacagc ctcgcttaca gattcgccga gcctgctgaa
      541 ttcgtcgttc agcattttct ctgtagattc ggctctctct ttcagctttt tctcgaactc
      601 cgcgcccgtc cgtaaaagat tgctcataaa acgctccttt cagtctgata ttcctcccgc
      661 cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct gaccacgtca agccccgcct
      721 ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc tccgtaatgg taaagcacct
      781 ccagacctcg tgtaacgctc tcagcaagcg cctgtttcgt ttccggcagg ttatcaggga
      841 gtgtcagcgt cctgcggttc tccggggcgt tggggtcatg cagcccgtaa tggtgattca
      901 ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac ggctggaggc
      961 gttttccgct ctgtagctcc atgttcggaa tgacaaaatt cagctcaaga cgccccttgt
     1021 cctggtgctc cacccacaga atgctgtact gatttttttc gagaccgggc atcagtacgc
     1081 gctcaaagct ctccatcact ttttcacgtc ctcccggcgg cagttccttc tctgcgaacg
     1141 acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc atcaataagc tcccggactt
     1201 cttccgggtt accctgaagt accgttgcac cttcccggtt acgctccctc cccagaaggt
     1261 aatcaaccgg accactgcca ccaccttttc cccgggcatg aaacttaact atcatcccgc
     1321 gctccctgtt ccctgacgac ctgtcgtaag ctgcgcaact ctctctcgat ggccatcagt
     1381 gcgaccacca cctgaacccg gtcgccggaa gaccactgcc cgctgttcac cttcctcgcc
     1441 gtctgattaa ggttattccc gatggcagcc agctgacgca gtaacggcgg tgccagtgtc
     1501 ggcagcttcc cggaacgggc aaccggctca cccaggcaga cccgccgcat ccataccgcc
     1561 agttgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     1621 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     1681 acggaggcat cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     1741 agccagacat taacgctgct ggagaaactc aacgaactgg acgcggatga acaggcagac
     1801 atctgtgaat ctcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgcttc
     1861 ggggatgacg gtgaaaccct ctgacacaag cagctcccgg agacggtcac agcttgtctg
     1921 tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagggggttt cggggcgaag
     1981 ccctgaacca gtcacgtagc gatagcggag tgtatactgg cttaaccatg cggcatcagt
     2041 gcagattgta tgaaaagtgc gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag
     2101 aaaatgcagc atcagacgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt
     2161 tcggctgcgg cgagcggtat cagctcactc aaaaacggta atgcggttat ccacagaatc
     2221 aggggataac accaaaagaa acatgtgagc aaaaaacaag aacccggaaa aggccacgcc
     2281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaacc gacgctcaag
     2341 tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc tggtggctcc
     2401 ctcgtgcgct ctcctgttcc tgccattcgg tttaccggtg tcactccgct gttatggccg
     2461 cgtttatctc attccacgcc tgacactcgg ttccgggtag gcagttcgct ccaagctgga
     2521 ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta actatccact
     2581 tgagtccaaa ccggaaagac acgacaaagc gccactggca gcagccattg gtaacttggt
     2641 tcgcagatga tttgttagct aaacacgagg ttgctcttga agtgtgcgcc tcagtacggc
     2701 tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggtccaga
     2761 aacttgccgg tttactgaac cttcgaaaaa accaccttgc cgggtggttt ttccgttttc
     2821 agaccaagag attacgcgca gaaaaagatg atctcaagaa gatccttttg attagtgccg
     2881 tcatttgact gaacatccca tgctaagcac ctagttgtaa gcccaagcga agatcatgta
     2941 tacttagttc aaggtgattt gctaccttta agtgcagcta gaaatttagg tgatttgcta
     3001 cctttaagtg cagctagaaa tttaggtgat ttgctacctt taagtgcagc tagaaattta
     3061 ggtgatttgc tacctttaag tgcagctaga aattcaggtg atttgctacc tttaagtgca
     3121 gctagaaatt taggtcattt actaccttaa agtaataaag taaaaaaggc gactatacac
     3181 agtcgccttt tttacgagaa aaaatgaaat tttacacaat atcaagcagt tacataaaat
     3241 acctgaagga ctttgatgac aaagttccca atagcgaaga tcctacatac aacaatccta
     3301 aggctttcat tggcatagta ctagagattg aaggacataa atatttagca cctttaacat
     3361 cgccaaaagc atggcatgct aatgtaaaag agtcatcacc cgccttcttc aaacttcatg
     3421 aaaacggtgt ccctgataat cagcttggat tgattaatct gaaatttatg attccaataa
     3481 tcgaagctga agtgtcttta cttgatctgg atagcatgcc tgatacgcct tacaaaagaa
     3541 tgctatataa acagttacaa tttattcgtg taaatgaaga taaaatatct gaaaaatcaa
     3601 aactattaag aaaccttgcc ttgcaaggca gaatgcaagg aacatgcgac tttgccgtgt
     3661 tagaagaaaa atatcaacat ttcggtaaaa aacctgaaga catggaaata gacgattaac
     3721 cattcttttt tatggaattg aaggtccaaa atcaagcggt tttggaccta ttttttttgc
     3781 tctcatccaa tataagaatg agatattcta attttgattt cgagaagcaa tcaaaaaggc
     3841 aagtcctcct ctgaaagatt aagcacctct gatttccttt tgtaagtgag agcgctgtta
     3901 cttcccgctc taaagtagaa gtaacatgaa tcatgaaaca tttccaacgg cttgttttct
     3961 attgattttt taacaatcct atcaagtaaa ccgatatgaa taagataggt aatagcttta
     4021 tttagttcag gtagtttgca tttcagtttt ttacatattg tttttttcct ggcaagagta
     4081 tactcatttc tattatctcg agcataaaga aggtacatgt aggtttgtaa agctagcaac
     4141 tcaaatggat atctgttgtg catggattga aatgcactta tttttccatc ttcacctaca
     4201 attcctttaa agggaagctt acaccaacca tcatcatgct tctctaatac atctatggta
     4261 taaatttttt tacgtactgt cgttgataaa ttttttatca actcttttgc ataaagaatt
     4321 ttaagccctt cgttaactaa cgatcggctt aatgatgcta tctcacatag gttactaaaa
     4381 gttagctcaa cagttctctg tcctgaatct gactctttgc agaaaaggca aaatgttaaa
     4441 taaatcttta acgcggcgat ggcttttgct gtatcaagat cgttgaattc agatattttg
     4501 atacctatcc atagtgaagg cattcttttc cagtacagca taaatcacct cattcccaga
     4561 atttgaaaaa tgaaatagac caaccccaat acagacatag atagcaccag attagttagc
     4621 accactgcga ccttcgcaag cctttcagcc aaaaacacac ctttctcaaa cattttttat
     4681 ccctcctgat agctttgttt ttttgattat ctcaggctgt gaaggcgttg taacgccttc
     4741 acaaaaatct atctagaaag ataagataag gcaaaagaaa gatatctata tgttatataa
     4801 gatattttat cttgcttttg gtattcttag aaatcatatt atggttcatg gtgaaccgta
     4861 aatgcagtgc tgatgctttt ctggttacgt taaatgtttt cacattagga ctttagatca
     4921 gaacaaacaa agcgttgtgc cttgcaagcc accctgcata aagcactaaa tttctttatc
     4981 tcattaaatt caccttgcca aaaggagcaa taatccctac cttacaactt taacaaaact
     5041 aaaaatcttt agcggactta gccgccgagt atagagaaat atgccatgcc taaaaaaaca
     5101 tcaaaaccaa acgatttaag caacactatc aacaacataa agaaagaaat taactcaggt
     5161 tttactgagt tacttaatcg cgtagaagcc ttagaggcta gtgacgcaca acacagcatg
     5221 gcgatacgtg atttacaaat tcaggctaga gccgctcgcg gagacaagcg catggatata
     5281 gcaagagact ttggcctcag cgagggacga attagccaga tcgttaatgc gggtcgtaac
     5341 taagacagac ttatgaaaaa tgaagacgac tacaaaacag gatggacgac tcagacaaca
     5401 aacccggcaa cagggaaaaa atgttctgga ggcgcagcca gaaacttaag gatatatcaa
     5461 gccggtggag caaactccgt gcgggtaaaa gctgcaatcg agggtgtcca gtcaatacag
     5521 ccaatcatcg atatgcaaca gtcacaaatc gagcaacaac aaactcaaat agcaatgctg
     5581 actcaatctt tgtctcaggc aatcaatgag ctaacaaaaa gcaggaatca ataggctata
     5641 aaggatgtta tttaccataa catccgttgc ccgcaccgcc ccggacagct tcgccgcgaa
     5701 cggggcggca gtcagccaaa accaacaaca aatcacccga aaagctaccg cgatcgcgcc
     5761 tgataaattt taaccgtatg aatatctatg caaccagatg gtgcagacta cattagcccg
     5821 tcaaaatcca ctgaatctgc cattttttat agtttcgcca tcacagcgac aacgaaggac
     5881 catccagcgt gcgttcccgt tccagggata acgcatgttc gcgctcatgt atcaaccgtt
     5941 cctctcttat ctccagttct cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa
     6001 gtgctgcacc tgctgactca actgcatgac ccgctcgttc agcatcgcgt tgtcccgttg
     6061 cgtaagcgaa aacatctt
//
