LOCUS       NZ_CP028639             6078 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain 133 plasmid pTA133-2, complete sequence.
ACCESSION   NZ_CP028639
VERSION     NZ_CP028639.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08773040
            Assembly: GCF_005221705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     WGA of Escherichia coli O157:H7: comparison of multiple isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-OCT-2017
            Assembly Method                   :: HGAP v. RS_HGAP_Assembly.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 240x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005221705.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,441
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 109 of 288
            Pseudo Genes (incomplete)         :: 163 of 288
            Pseudo Genes (internal stop)      :: 70 of 288
            Pseudo Genes (multiple problems)  :: 44 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028639.1.
            Bacteria available from Dr. Terry Arthur lab at the U.S Meat Animal
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="133"
                     /serotype="O157:H7"
                     /isolation_source="cattle"
                     /db_xref="taxon:562"
                     /plasmid="pTA133-2"
                     /collection_date="2006"
                     /collected_by="USMARC"
     gene            complement(298..531)
                     /locus_tag="C9E57_RS27755"
     CDS             complement(298..531)
                     /locus_tag="C9E57_RS27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029401705.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(538..1056)
                     /locus_tag="C9E57_RS27760"
     CDS             complement(538..1056)
                     /locus_tag="C9E57_RS27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_043991090.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1094..1744)
                     /locus_tag="C9E57_RS29020"
                     /pseudo=""
     CDS             complement(<1094..1744)
                     /locus_tag="C9E57_RS29020"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1734..2057)
                     /locus_tag="C9E57_RS27770"
                     /old_locus_tag="C9E57_29050"
     CDS             complement(1734..2057)
                     /locus_tag="C9E57_RS27770"
                     /old_locus_tag="C9E57_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001508824.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2122..2313
                     /locus_tag="C9E57_RS27775"
                     /old_locus_tag="C9E57_29055"
     CDS             2122..2313
                     /locus_tag="C9E57_RS27775"
                     /old_locus_tag="C9E57_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2954..3079
                     /locus_tag="C9E57_RS29330"
                     /old_locus_tag="C9E57_29070"
     CDS             2954..3079
                     /locus_tag="C9E57_RS29330"
                     /old_locus_tag="C9E57_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071535512.1"
                     /translation="MHEPPVQSDCCALSGNYPLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     gene            3388..3579
                     /locus_tag="C9E57_RS27800"
     CDS             3388..3579
                     /locus_tag="C9E57_RS27800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137049521.1"
                     /translation="MLPLSAARNLGDLLPLSAARNLGDLLPLSAARNLGDLLPLSAARN
                     SGDLLPLSAARNLGHLLP"
                     /name="hypothetical protein"
     gene            3633..4148
                     /locus_tag="C9E57_RS27805"
                     /old_locus_tag="C9E57_29080"
     CDS             3633..4148
                     /locus_tag="C9E57_RS27805"
                     /old_locus_tag="C9E57_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_000675353.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(4260..4970)
                     /locus_tag="C9E57_RS27810"
                     /old_locus_tag="C9E57_29085"
     CDS             complement(4260..4970)
                     /locus_tag="C9E57_RS27810"
                     /old_locus_tag="C9E57_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177388.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            5515..5772
                     /locus_tag="C9E57_RS27815"
                     /old_locus_tag="C9E57_29090"
     CDS             5515..5772
                     /locus_tag="C9E57_RS27815"
                     /old_locus_tag="C9E57_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001508827.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            5782..6063
                     /locus_tag="C9E57_RS27820"
                     /old_locus_tag="C9E57_29095"
     CDS             5782..6063
                     /locus_tag="C9E57_RS27820"
                     /old_locus_tag="C9E57_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177387.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     Typing          complement(2392..2476)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          2776..3293
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(222..1744)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.762"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atttaccata acatccgttg cccgcaccgc cccggacagc ttcgccgcga acggggcggc
       61 agtcagccaa aaccaacaac aaatcacccg aaaagctacc gcgatcgcgc ctgataaatt
      121 ttaaccgtat gaatatctat gcaaccagat ggtgcagact acattagccc gtcaaaatcc
      181 actgaatctg ccatttttta tagtttcgcc atcacagcga caacgaagga ccatccagcg
      241 tgcgttcccg ttccagggat aacgcatgtt cgcgctcatg tatcaaccgt tcctctctta
      301 tctccagttc tcgctgtata actggctcaa gcgttctgtc cgctcgctca agtgctgcac
      361 ctgctgactc aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga
      421 aaacatcttc tgcaattcca cgaaggcgct ctcccattcg ctcagccgct gcatatagtc
      481 ctgttgcagc tgctctaagg cgttcagcaa atgtgtttcc agctctgtca ctctgtatca
      541 ctccttcaga tgtacccatt ccttcccctg aaaggaaatc acctctgctg acttcccgta
      601 aggaaggacc agaaatttcc tgttcccgtc ctgcacaaac tccacgcccc atgtcttcgc
      661 gttcagtttc tgtaatgtct cttcctgctt cctgatatct tccaggttcg cctgtatcct
      721 cccgccaaga taccagagcg tcccgccact cgcggtaaac aggagaaaga ctatccccag
      781 taacatcatg cccgtattcc ctgccagctt taacacgtcc ctcctgtgct gcatcatcgc
      841 ctctttcacc ccttcccggt gtttttccag cgattcctcc gtcgaggccg taaaccgggc
      901 tatagcgcct ttgattttcg tctcgtttga ggtcacagcc tcgcttacag attcgccgag
      961 cctgctgaat tcgtcgttca gcattttctc tgtagattcg gctctctctt tcagcttttt
     1021 ctcgaactcc gcgcccgtcc gtaaaagatt gctcataaaa cgctcctttc agtctgatat
     1081 tcctcccgcc gttcgggtct gcaatgctga tactgcttcg cgtcaccctg accacgtcaa
     1141 gccccgcctc tgtgagcgcc tgaatcacat cctgacggcc ttttatctct ccgtaatggt
     1201 aaagcacctc cagacctcgt gtaacgctct cagcaagcgc ctgtttcgtt tccggcaggt
     1261 tatcagggag tgtcagcgtc ctgcggttct ccggggcgtt ggggtcatgc agcccgtaat
     1321 ggtgattcac cagcgtctgc caggcatcaa ttctcggcct gtctgcccgg tcgtagtacg
     1381 gctggaggcg ttttccgctc tgtagctcca tgttcggaat gacaaaattc agctcaagac
     1441 gccccttgtc ctggtgctcc acccacagaa tgctgtactg atttttttcg agaccgggca
     1501 tcagtacgcg ctcaaagctc tccatcactt tttcacgtcc tcccggcggc agttccttct
     1561 ctgcgaacga cagaacaccg gacgtgtatt tcttcgcaaa tggcgtggca tcaataagct
     1621 cccggacttc ttccgggtta ccctgaagta ccgttgcacc ttcccggtta cgctccctcc
     1681 ccagaaggta atcaaccgga ccactgccac caccttttcc ccgggcatga aacttaacta
     1741 tcatcccgcg ctccctgttc cctgacgacc tgtcgtaagc tgcgcaactc tctctcgatg
     1801 gccatcagtg cgaccaccac ctgaacccgg tcgccggaag accactgccc gctgttcacc
     1861 ttcctcgccg tctgattaag gttattcccg atggcagcca gctgacgcag taacggcggt
     1921 gccagtgtcg gcagcttccc ggaacgggca accggctcac ccaggcagac ccgccgcatc
     1981 cataccgcca gttgtttacc ctcacagcgt tccagtaacc gggcatgttc atcatcagta
     2041 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     2101 cccccttaca cggaggcatc agtgactaaa caggaaaaaa ccgcccttaa catggcccgc
     2161 tttatcagaa gccagacatt aacgctgctg gagaaactca acgaactgga cgcggatgaa
     2221 caggcagaca tctgtgaatc tcttcacgac cacgccgatg agctttaccg cagctgcctc
     2281 gcacgcttcg gggatgacgg tgaaaccctc tgacacaagc agctcccgga gacggtcaca
     2341 gcttgtctgt gagcggatgc cgggagcaga caagcccgtc agggcgcgtc agggggtttc
     2401 ggggcgaagc cctgaaccag tcacgtagcg atagcggagt gtatactggc ttaaccatgc
     2461 ggcatcagtg cagattgtat gaaaagtgcg ccatgccggg tgtgaaatgc cgcacagatg
     2521 cgtaaggaga aaatgcagca tcagacgctc ttccgcttcc tcgctcactg actcgctacg
     2581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaaacggtaa tgcggttatc
     2641 cacagaatca ggggataaca ccaaaagaaa catgtgagca aaaaacaaga acccggaaaa
     2701 ggccacgccg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaaccg
     2761 acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc gtttccccct
     2821 ggtggctccc tcgtgcgctc tcctgttcct gccattcggt ttaccggtgt cactccgctg
     2881 ttatggccgc gtttatctca ttccacgcct gacactcggt tccgggtagg cagttcgctc
     2941 caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa
     3001 ctatccactt gagtccaaac cggaaagaca cgacaaagcg ccactggcag cagccattgg
     3061 taacttggtt cgcagatgat ttgttagcta aacacgaggt tgctcttgaa gtgtgcgcct
     3121 cagtacggct acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca
     3181 cggtccagaa acttgccggt ttactgaacc ttcgaaaaaa ccaccttgcc gggtggtttt
     3241 tccgttttca gaccaagaga ttacgcgcag aaaaagatga tctcaagaag atccttttga
     3301 ttagtgccgt catttgactg aacatcccat gctaagcacc tagttgtaag cccaagcgaa
     3361 gatcatgtat acttagttca aggtgatttg ctacctttaa gtgcagctag aaatttaggt
     3421 gatttgctac ctttaagtgc agctagaaat ttaggtgatt tgctaccttt aagtgcagct
     3481 agaaatttag gtgatttgct acctttaagt gcagctagaa attcaggtga tttgctacct
     3541 ttaagtgcag ctagaaattt aggtcattta ctaccttaaa gtaataaagt aaaaaaggcg
     3601 actatacaca gtcgcctttt ttacgagaaa aaatgaaatt ttacacaata tcaagcagtt
     3661 acataaaata cctgaaggac tttgatgaca aagttcccaa tagcgaagat cctacataca
     3721 acaatcctaa ggctttcatt ggcatagtac tagagattga aggacataaa tatttagcac
     3781 ctttaacatc gccaaaagca tggcatgcta atgtaaaaga gtcatcaccc gccttcttca
     3841 aacttcatga aaacggtgtc cctgataatc agcttggatt gattaatctg aaatttatga
     3901 ttccaataat cgaagctgaa gtgtctttac ttgatctgga tagcatgcct gatacgcctt
     3961 acaaaagaat gctatataaa cagttacaat ttattcgtgt aaatgaagat aaaatatctg
     4021 aaaaatcaaa actattaaga aaccttgcct tgcaaggcag aatgcaagga acatgcgact
     4081 ttgccgtgtt agaagaaaaa tatcaacatt tcggtaaaaa acctgaagac atggaaatag
     4141 acgattaacc attctttttt atggaattga aggtccaaaa tcaagcggtt ttggacctat
     4201 tttttttgct ctcatccaat ataagaatga gatattctaa ttttgatttc gagaagcaat
     4261 caaaaaggca agtcctcctc tgaaagatta agcacctctg atttcctttt gtaagtgaga
     4321 gcgctgttac ttcccgctct aaagtagaag taacatgaat catgaaacat ttccaacggc
     4381 ttgttttcta ttgatttttt aacaatccta tcaagtaaac cgatatgaat aagataggta
     4441 atagctttat ttagttcagg tagtttgcat ttcagttttt tacatattgt ttttttcctg
     4501 gcaagagtat actcatttct attatctcga gcataaagaa ggtacatgta ggtttgtaaa
     4561 gctagcaact caaatggata tctgttgtgc atggattgaa atgcacttat ttttccatct
     4621 tcacctacaa ttcctttaaa gggaagctta caccaaccat catcatgctt ctctaataca
     4681 tctatggtat aaattttttt acgtactgtc gttgataaat tttttatcaa ctcttttgca
     4741 taaagaattt taagcccttc gttaactaac gatcggctta atgatgctat ctcacatagg
     4801 ttactaaaag ttagctcaac agttctctgt cctgaatctg actctttgca gaaaaggcaa
     4861 aatgttaaat aaatctttaa cgcggcgatg gcttttgctg tatcaagatc gttgaattca
     4921 gatattttga tacctatcca tagtgaaggc attcttttcc agtacagcat aaatcacctc
     4981 attcccagaa tttgaaaaat gaaatagacc aaccccaata cagacataga tagcaccaga
     5041 ttagttagca ccactgcgac cttcgcaagc ctttcagcca aaaacacacc tttctcaaac
     5101 attttttatc cctcctgata gctttgtttt tttgattatc tcaggctgtg aaggcgttgt
     5161 aacgccttca caaaaatcta tctagaaaga taagataagg caaaagaaag atatctatat
     5221 gttatataag atattttatc ttgcttttgg tattcttaga aatcatatta tggttcatgg
     5281 tgaaccgtaa atgcagtgct gatgcttttc tggttacgtt aaatgttttc acattaggac
     5341 tttagatcag aacaaacaaa gcgttgtgcc ttgcaagcca ccctgcataa agcactaaat
     5401 ttctttatct cattaaattc accttgccaa aaggagcaat aatccctacc ttacaacttt
     5461 aacaaaacta aaaatcttta gcggacttag ccgccgagta tagagaaata tgccatgcct
     5521 aaaaaaacat caaaaccaaa cgatttaagc aacactatca acaacataaa gaaagaaatt
     5581 aactcaggtt ttactgagtt acttaatcgc gtagaagcct tagaggctag tgacgcacaa
     5641 cacagcatgg cgatacgtga tttacaaatt caggctagag ccgctcgcgg agacaagcgc
     5701 atggatatag caagagactt tggcctcagc gagggacgaa ttagccagat cgttaatgcg
     5761 ggtcgtaact aagacagact tatgaaaaat gaagacgact acaaaacagg atggacgact
     5821 cagacaacaa acccggcaac agggaaaaaa tgttctggag gcgcagccag aaacttaagg
     5881 atatatcaag ccggtggagc aaactccgtg cgggtaaaag ctgcaatcga gggtgtccag
     5941 tcaatacagc caatcatcga tatgcaacag tcacaaatcg agcaacaaca aactcaaata
     6001 gcaatgctga ctcaatcttt gtctcaggca atcaatgagc taacaaaaag caggaatcaa
     6061 taggctataa aggatgtt
//
