LOCUS       NZ_CP028633             6078 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain 135 plasmid pTA135-2, complete sequence.
ACCESSION   NZ_CP028633
VERSION     NZ_CP028633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08773042
            Assembly: GCF_005221665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     WGA of Escherichia coli O157:H7: comparison of multiple isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Arthur,T.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-OCT-2017
            Assembly Method                   :: HGAP v. RS_HGAP_Assembly.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005221665.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:50:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,152
            CDSs (with protein)               :: 5,152
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 109 of 288
            Pseudo Genes (incomplete)         :: 163 of 288
            Pseudo Genes (internal stop)      :: 70 of 288
            Pseudo Genes (multiple problems)  :: 44 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028633.1.
            Bacteria available from Dr. Terry Arthur lab at the U.S Meat Animal
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="135"
                     /serotype="O157:H7"
                     /isolation_source="cattle"
                     /db_xref="taxon:562"
                     /plasmid="pTA135-2"
                     /collection_date="2006"
                     /collected_by="USMARC"
     gene            complement(join(5805..6078,1..245))
                     /locus_tag="C9E59_RS27755"
     CDS             complement(join(5805..6078,1..245))
                     /locus_tag="C9E59_RS27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_043991090.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<283..933)
                     /locus_tag="C9E59_RS29025"
                     /pseudo=""
     CDS             complement(<283..933)
                     /locus_tag="C9E59_RS29025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(923..1246)
                     /locus_tag="C9E59_RS27765"
                     /old_locus_tag="C9E59_29045"
     CDS             complement(923..1246)
                     /locus_tag="C9E59_RS27765"
                     /old_locus_tag="C9E59_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001508824.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1311..1502
                     /locus_tag="C9E59_RS27770"
                     /old_locus_tag="C9E59_29050"
     CDS             1311..1502
                     /locus_tag="C9E59_RS27770"
                     /old_locus_tag="C9E59_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2143..2268
                     /locus_tag="C9E59_RS29335"
                     /old_locus_tag="C9E59_29065"
     CDS             2143..2268
                     /locus_tag="C9E59_RS29335"
                     /old_locus_tag="C9E59_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071535512.1"
                     /translation="MHEPPVQSDCCALSGNYPLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     gene            2577..2768
                     /locus_tag="C9E59_RS27795"
     CDS             2577..2768
                     /locus_tag="C9E59_RS27795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137049521.1"
                     /translation="MLPLSAARNLGDLLPLSAARNLGDLLPLSAARNLGDLLPLSAARN
                     SGDLLPLSAARNLGHLLP"
                     /name="hypothetical protein"
     gene            2822..3337
                     /locus_tag="C9E59_RS27800"
                     /old_locus_tag="C9E59_29075"
     CDS             2822..3337
                     /locus_tag="C9E59_RS27800"
                     /old_locus_tag="C9E59_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_000675353.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(3449..4159)
                     /locus_tag="C9E59_RS27805"
                     /old_locus_tag="C9E59_29080"
     CDS             complement(3449..4159)
                     /locus_tag="C9E59_RS27805"
                     /old_locus_tag="C9E59_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177388.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            4704..4961
                     /locus_tag="C9E59_RS27810"
                     /old_locus_tag="C9E59_29085"
     CDS             4704..4961
                     /locus_tag="C9E59_RS27810"
                     /old_locus_tag="C9E59_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001508827.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            4971..5252
                     /locus_tag="C9E59_RS27815"
                     /old_locus_tag="C9E59_29090"
     CDS             4971..5252
                     /locus_tag="C9E59_RS27815"
                     /old_locus_tag="C9E59_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177387.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     gene            complement(5565..5798)
                     /locus_tag="C9E59_RS27820"
     CDS             complement(5565..5798)
                     /locus_tag="C9E59_RS27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029401705.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1581..1665)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          1965..2482
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..933)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.248"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5489..6078)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.204"
                     /coverage="99.0"
                     /evalue="3.72e-82"
ORIGIN
        1 aacacgtccc tcctgtgctg catcatcgcc tctttcaccc cttcccggtg tttttccagc
       61 gattcctccg tcgaggccgt aaaccgggct atagcgcctt tgattttcgt ctcgtttgag
      121 gtcacagcct cgcttacaga ttcgccgagc ctgctgaatt cgtcgttcag cattttctct
      181 gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtccg taaaagattg
      241 ctcataaaac gctcctttca gtctgatatt cctcccgccg ttcgggtctg caatgctgat
      301 actgcttcgc gtcaccctga ccacgtcaag ccccgcctct gtgagcgcct gaatcacatc
      361 ctgacggcct tttatctctc cgtaatggta aagcacctcc agacctcgtg taacgctctc
      421 agcaagcgcc tgtttcgttt ccggcaggtt atcagggagt gtcagcgtcc tgcggttctc
      481 cggggcgttg gggtcatgca gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat
      541 tctcggcctg tctgcccggt cgtagtacgg ctggaggcgt tttccgctct gtagctccat
      601 gttcggaatg acaaaattca gctcaagacg ccccttgtcc tggtgctcca cccacagaat
      661 gctgtactga tttttttcga gaccgggcat cagtacgcgc tcaaagctct ccatcacttt
      721 ttcacgtcct cccggcggca gttccttctc tgcgaacgac agaacaccgg acgtgtattt
      781 cttcgcaaat ggcgtggcat caataagctc ccggacttct tccgggttac cctgaagtac
      841 cgttgcacct tcccggttac gctccctccc cagaaggtaa tcaaccggac cactgccacc
      901 accttttccc cgggcatgaa acttaactat catcccgcgc tccctgttcc ctgacgacct
      961 gtcgtaagct gcgcaactct ctctcgatgg ccatcagtgc gaccaccacc tgaacccggt
     1021 cgccggaaga ccactgcccg ctgttcacct tcctcgccgt ctgattaagg ttattcccga
     1081 tggcagccag ctgacgcagt aacggcggtg ccagtgtcgg cagcttcccg gaacgggcaa
     1141 ccggctcacc caggcagacc cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt
     1201 ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
     1261 tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca gtgactaaac
     1321 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgctgctgg
     1381 agaaactcaa cgaactggac gcggatgaac aggcagacat ctgtgaatct cttcacgacc
     1441 acgccgatga gctttaccgc agctgcctcg cacgcttcgg ggatgacggt gaaaccctct
     1501 gacacaagca gctcccggag acggtcacag cttgtctgtg agcggatgcc gggagcagac
     1561 aagcccgtca gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt cacgtagcga
     1621 tagcggagtg tatactggct taaccatgcg gcatcagtgc agattgtatg aaaagtgcgc
     1681 catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa aatgcagcat cagacgctct
     1741 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggtatca
     1801 gctcactcaa aaacggtaat gcggttatcc acagaatcag gggataacac caaaagaaac
     1861 atgtgagcaa aaaacaagaa cccggaaaag gccacgccgc tggcgttttt ccataggctc
     1921 cgcccccctg acgagcatca caaaaaccga cgctcaagtc agaggtggcg aaacccgaca
     1981 ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg
     2041 ccattcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat tccacgcctg
     2101 acactcggtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     2161 cagtccgact gctgcgcctt atccggtaac tatccacttg agtccaaacc ggaaagacac
     2221 gacaaagcgc cactggcagc agccattggt aacttggttc gcagatgatt tgttagctaa
     2281 acacgaggtt gctcttgaag tgtgcgcctc agtacggcta cactggaagg acagatttgg
     2341 ttgctgtgct ctgcgaaagc cagttaccac ggtccagaaa cttgccggtt tactgaacct
     2401 tcgaaaaaac caccttgccg ggtggttttt ccgttttcag accaagagat tacgcgcaga
     2461 aaaagatgat ctcaagaaga tccttttgat tagtgccgtc atttgactga acatcccatg
     2521 ctaagcacct agttgtaagc ccaagcgaag atcatgtata cttagttcaa ggtgatttgc
     2581 tacctttaag tgcagctaga aatttaggtg atttgctacc tttaagtgca gctagaaatt
     2641 taggtgattt gctaccttta agtgcagcta gaaatttagg tgatttgcta cctttaagtg
     2701 cagctagaaa ttcaggtgat ttgctacctt taagtgcagc tagaaattta ggtcatttac
     2761 taccttaaag taataaagta aaaaaggcga ctatacacag tcgccttttt tacgagaaaa
     2821 aatgaaattt tacacaatat caagcagtta cataaaatac ctgaaggact ttgatgacaa
     2881 agttcccaat agcgaagatc ctacatacaa caatcctaag gctttcattg gcatagtact
     2941 agagattgaa ggacataaat atttagcacc tttaacatcg ccaaaagcat ggcatgctaa
     3001 tgtaaaagag tcatcacccg ccttcttcaa acttcatgaa aacggtgtcc ctgataatca
     3061 gcttggattg attaatctga aatttatgat tccaataatc gaagctgaag tgtctttact
     3121 tgatctggat agcatgcctg atacgcctta caaaagaatg ctatataaac agttacaatt
     3181 tattcgtgta aatgaagata aaatatctga aaaatcaaaa ctattaagaa accttgcctt
     3241 gcaaggcaga atgcaaggaa catgcgactt tgccgtgtta gaagaaaaat atcaacattt
     3301 cggtaaaaaa cctgaagaca tggaaataga cgattaacca ttctttttta tggaattgaa
     3361 ggtccaaaat caagcggttt tggacctatt ttttttgctc tcatccaata taagaatgag
     3421 atattctaat tttgatttcg agaagcaatc aaaaaggcaa gtcctcctct gaaagattaa
     3481 gcacctctga tttccttttg taagtgagag cgctgttact tcccgctcta aagtagaagt
     3541 aacatgaatc atgaaacatt tccaacggct tgttttctat tgatttttta acaatcctat
     3601 caagtaaacc gatatgaata agataggtaa tagctttatt tagttcaggt agtttgcatt
     3661 tcagtttttt acatattgtt tttttcctgg caagagtata ctcatttcta ttatctcgag
     3721 cataaagaag gtacatgtag gtttgtaaag ctagcaactc aaatggatat ctgttgtgca
     3781 tggattgaaa tgcacttatt tttccatctt cacctacaat tcctttaaag ggaagcttac
     3841 accaaccatc atcatgcttc tctaatacat ctatggtata aattttttta cgtactgtcg
     3901 ttgataaatt ttttatcaac tcttttgcat aaagaatttt aagcccttcg ttaactaacg
     3961 atcggcttaa tgatgctatc tcacataggt tactaaaagt tagctcaaca gttctctgtc
     4021 ctgaatctga ctctttgcag aaaaggcaaa atgttaaata aatctttaac gcggcgatgg
     4081 cttttgctgt atcaagatcg ttgaattcag atattttgat acctatccat agtgaaggca
     4141 ttcttttcca gtacagcata aatcacctca ttcccagaat ttgaaaaatg aaatagacca
     4201 accccaatac agacatagat agcaccagat tagttagcac cactgcgacc ttcgcaagcc
     4261 tttcagccaa aaacacacct ttctcaaaca ttttttatcc ctcctgatag ctttgttttt
     4321 ttgattatct caggctgtga aggcgttgta acgccttcac aaaaatctat ctagaaagat
     4381 aagataaggc aaaagaaaga tatctatatg ttatataaga tattttatct tgcttttggt
     4441 attcttagaa atcatattat ggttcatggt gaaccgtaaa tgcagtgctg atgcttttct
     4501 ggttacgtta aatgttttca cattaggact ttagatcaga acaaacaaag cgttgtgcct
     4561 tgcaagccac cctgcataaa gcactaaatt tctttatctc attaaattca ccttgccaaa
     4621 aggagcaata atccctacct tacaacttta acaaaactaa aaatctttag cggacttagc
     4681 cgccgagtat agagaaatat gccatgccta aaaaaacatc aaaaccaaac gatttaagca
     4741 acactatcaa caacataaag aaagaaatta actcaggttt tactgagtta cttaatcgcg
     4801 tagaagcctt agaggctagt gacgcacaac acagcatggc gatacgtgat ttacaaattc
     4861 aggctagagc cgctcgcgga gacaagcgca tggatatagc aagagacttt ggcctcagcg
     4921 agggacgaat tagccagatc gttaatgcgg gtcgtaacta agacagactt atgaaaaatg
     4981 aagacgacta caaaacagga tggacgactc agacaacaaa cccggcaaca gggaaaaaat
     5041 gttctggagg cgcagccaga aacttaagga tatatcaagc cggtggagca aactccgtgc
     5101 gggtaaaagc tgcaatcgag ggtgtccagt caatacagcc aatcatcgat atgcaacagt
     5161 cacaaatcga gcaacaacaa actcaaatag caatgctgac tcaatctttg tctcaggcaa
     5221 tcaatgagct aacaaaaagc aggaatcaat aggctataaa ggatgttatt taccataaca
     5281 tccgttgccc gcaccgcccc ggacagcttc gccgcgaacg gggcggcagt cagccaaaac
     5341 caacaacaaa tcacccgaaa agctaccgcg atcgcgcctg ataaatttta accgtatgaa
     5401 tatctatgca accagatggt gcagactaca ttagcccgtc aaaatccact gaatctgcca
     5461 ttttttatag tttcgccatc acagcgacaa cgaaggacca tccagcgtgc gttcccgttc
     5521 cagggataac gcatgttcgc gctcatgtat caaccgttcc tctcttatct ccagttctcg
     5581 ctgtataact ggctcaagcg ttctgtccgc tcgctcaagt gctgcacctg ctgactcaac
     5641 tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc
     5701 aattccacga aggcgctctc ccattcgctc agccgctgca tatagtcctg ttgcagctgc
     5761 tctaaggcgt tcagcaaatg tgtttccagc tctgtcactc tgtatcactc cttcagatgt
     5821 acccattcct tcccctgaaa ggaaatcacc tctgctgact tcccgtaagg aaggaccaga
     5881 aatttcctgt tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt cagtttctgt
     5941 aatgtctctt cctgcttcct gatatcttcc aggttcgcct gtatcctccc gccaagatac
     6001 cagagcgtcc cgccactcgc ggtaaacagg agaaagacta tccccagtaa catcatgccc
     6061 gtattccctg ccagcttt
//
