LOCUS       NZ_CP028580            12746 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP36 plasmid p2_020036, complete
            sequence.
ACCESSION   NZ_CP028580
VERSION     NZ_CP028580.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07235710
            Assembly: GCF_002811325.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020036
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 12746)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 12746)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:37:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,507
            Genes (coding)                    :: 5,369
            CDSs (with protein)               :: 5,369
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 45 of 138
            Pseudo Genes (incomplete)         :: 90 of 138
            Pseudo Genes (internal stop)      :: 15 of 138
            Pseudo Genes (multiple problems)  :: 12 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028580.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028580.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP36"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_020036"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017"
     gene            47..>1195
                     /locus_tag="CEA75_RS00165"
                     /old_locus_tag="CEA75_00185"
                     /pseudo=""
     CDS             47..>1195
                     /locus_tag="CEA75_RS00165"
                     /old_locus_tag="CEA75_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1252..1949
                     /locus_tag="CEA75_RS00170"
                     /old_locus_tag="CEA75_00190"
     CDS             join(1252..1501,1501..1949)
                     /locus_tag="CEA75_RS00170"
                     /old_locus_tag="CEA75_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            <1966..2508
                     /locus_tag="CEA75_RS00175"
                     /old_locus_tag="CEA75_00195"
                     /pseudo=""
     CDS             <1966..2508
                     /locus_tag="CEA75_RS00175"
                     /old_locus_tag="CEA75_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            2518..2775
                     /locus_tag="CEA75_RS00180"
                     /old_locus_tag="CEA75_00200"
     CDS             2518..2775
                     /locus_tag="CEA75_RS00180"
                     /old_locus_tag="CEA75_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2860..3009
                     /locus_tag="CEA75_RS00185"
                     /old_locus_tag="CEA75_00205"
     CDS             2860..3009
                     /locus_tag="CEA75_RS00185"
                     /old_locus_tag="CEA75_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4493..6457
                     /locus_tag="CEA75_RS00190"
                     /old_locus_tag="CEA75_00215"
     CDS             4493..6457
                     /locus_tag="CEA75_RS00190"
                     /old_locus_tag="CEA75_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6457..7188
                     /gene="mobC"
                     /locus_tag="CEA75_RS00195"
                     /old_locus_tag="CEA75_00220"
     CDS             6457..7188
                     /gene="mobC"
                     /locus_tag="CEA75_RS00195"
                     /old_locus_tag="CEA75_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7195..7725
                     /locus_tag="CEA75_RS00200"
                     /old_locus_tag="CEA75_00225"
     CDS             7195..7725
                     /locus_tag="CEA75_RS00200"
                     /old_locus_tag="CEA75_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7752..7931)
                     /locus_tag="CEA75_RS00205"
                     /old_locus_tag="CEA75_00230"
     CDS             complement(7752..7931)
                     /locus_tag="CEA75_RS00205"
                     /old_locus_tag="CEA75_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7957..8385)
                     /locus_tag="CEA75_RS00210"
                     /old_locus_tag="CEA75_00235"
     CDS             complement(7957..8385)
                     /locus_tag="CEA75_RS00210"
                     /old_locus_tag="CEA75_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8549..8863)
                     /locus_tag="CEA75_RS00215"
                     /old_locus_tag="CEA75_00240"
     CDS             complement(8549..8863)
                     /locus_tag="CEA75_RS00215"
                     /old_locus_tag="CEA75_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8851..9129)
                     /locus_tag="CEA75_RS00220"
                     /old_locus_tag="CEA75_00245"
     CDS             complement(8851..9129)
                     /locus_tag="CEA75_RS00220"
                     /old_locus_tag="CEA75_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9304..9669)
                     /locus_tag="CEA75_RS27995"
                     /old_locus_tag="CEA75_00250"
     CDS             complement(9304..9669)
                     /locus_tag="CEA75_RS27995"
                     /old_locus_tag="CEA75_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(9666..10037)
                     /gene="tolA"
                     /locus_tag="CEA75_RS00230"
                     /old_locus_tag="CEA75_00255"
     CDS             complement(9666..10037)
                     /gene="tolA"
                     /locus_tag="CEA75_RS00230"
                     /old_locus_tag="CEA75_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(10311..10556)
                     /locus_tag="CEA75_RS00235"
                     /old_locus_tag="CEA75_00260"
     CDS             complement(10311..10556)
                     /locus_tag="CEA75_RS00235"
                     /old_locus_tag="CEA75_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11201..12688
                     /gene="ltrA"
                     /locus_tag="CEA75_RS00240"
                     /old_locus_tag="CEA75_29035"
     CDS             11201..12688
                     /gene="ltrA"
                     /locus_tag="CEA75_RS00240"
                     /old_locus_tag="CEA75_29035"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6201
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3113..3691)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6458..7185
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgttggctt taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga
       61 cggtcgaggt ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt
      121 gaacggtacg tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
      181 gccgggctgg ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga
      241 gtttggtaac ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg
      301 tagctctggt ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc
      361 tactccgggg gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt
      421 tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat
      481 ctacggtgtg ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat
      541 gacgtcatta ccggccgatg cggtgacgga gactccggca agtactttac cactggacca
      601 ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat
      661 tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg
      721 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa
      781 aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg
      841 tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
      901 gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
      961 acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga
     1021 ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat
     1081 tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc
     1141 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgggtg
     1201 atgctaccaa cttgctgatt tagtgtataa tggtgttttt gaggtgctcc cgtggcttca
     1261 gtctccgtct gctgtccctc ctgttccgct actgaaggcg tgatgcgtaa tggcaaaagt
     1321 actgccgggc atcaacgtta tctctgctct cactgccgta aaacatggca gctcaccttc
     1381 acttatgccg cttctcagcc cggtacacac cagaaaatca ttgatatggc tatgaacggc
     1441 gtcggttgcc gtgccaccgc acgactaatg ggcgtgggcc tcaacaccat tttgcgccat
     1501 ttaaaaaact caggccgcag tcagtaacct cccggataca gccgggcagt gatgtcattg
     1561 tctgtgcgga gatggacgag caatggggct acgtcggtgc aaaatcacgt cagcgctggc
     1621 tgttttacgc gtatgacagg atacgcaggg cggtggtagc ccacgttttc ggtgaacgca
     1681 ctatggccac actggagcgt cttctgagcc tgctgtcagt ttttgacgtg gtggtatgga
     1741 tgacggacgg ctggccgatg tatgagtcac gtctgaaggg aaaactgcac gtcatcagta
     1801 agcgttacac gcagcgaatt gagcgacaca acctgaatct gagacagcat ctggcaaggc
     1861 tgggcaggaa gtccctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
     1921 actatctgaa cataaaacac tatcagtaaa ttggagtcat tacctgattt tgtgacttat
     1981 aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct tgataaggat
     2041 attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc aatgaatgaa
     2101 tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa acaaaaggag
     2161 aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc tcgcaagggg
     2221 actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat aaagggactt
     2281 ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg taagcgggcc
     2341 cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca cggtgagctt
     2401 gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa aacgggtaag
     2461 caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga ggtaaatatg
     2521 gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa aggggaagag
     2581 tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat gcctttaaag
     2641 gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt actacagcca
     2701 ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt tgattatcgg
     2761 gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact tccggtgttc
     2821 agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt aaagactatt
     2881 tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc aaattatatc
     2941 cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg
     3001 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctttt
     3061 gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg
     3121 gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca
     3181 gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct
     3241 ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct ttcccgggtt
     3301 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
     3361 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta
     3421 tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg taaacggcag
     3481 ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt
     3541 cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg
     3601 caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg
     3661 ccaccggcgc ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc
     3721 tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg
     3781 accgagtgag cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct
     3841 gatgtgggat attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat
     3901 aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt
     3961 tctccttgcg gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag
     4021 accttgctta cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct
     4081 gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
     4141 ccacccacaa aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat
     4201 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
     4261 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
     4321 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg
     4381 caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag
     4441 cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
     4501 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
     4561 ggtcagctgg ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct
     4621 gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa
     4681 ggcgcggcgg gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc
     4741 ggtgctggcc tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa
     4801 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
     4861 cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga
     4921 ttattacctg ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt
     4981 cgccggcgtc cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc
     5041 cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
     5101 cggagaaatg acagacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct
     5161 ggccgatttt tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga
     5221 gcagggccag ccggtgctgg tccctcgtga tacctggcgc aagaccaata ttcagatcct
     5281 cggcctgccc gggagcggca agagcgcgat ggccaccaac gcgctcattc gctgcgtacg
     5341 cgatttcggg gacgctgtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt
     5401 cttccgggcg cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     5461 gctgaatctg ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt
     5521 taacctgagt gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     5581 gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     5641 gtacgcgttg ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa
     5701 cgtggccgac ctgagcgtcc tgcagacgga cagcggtatc gatgtggcgg ggataatcaa
     5761 cggcggcggc tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca
     5821 gaagatgctg tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg
     5881 gccgcacgcg agcatcatgc tcgatgagat taaatatctg ctgtcgaagt acgtgctgaa
     5941 cgcgctgggg acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg
     6001 cgacttcggt cagtgcgggc aggatctgcc cgctgatttt gtgaaaacca cggtactgga
     6061 taacacgccg atccgctggt tctatcgcgc ggccagccag gagtccgcgc agtgggcggc
     6121 gggccagacc ggggagatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
     6181 gaacgtggag cacatcagcg gcgacagctt catccagaag gaggcgcggc cgctgtttga
     6241 cgtgaacacc ctgcaacacc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc
     6301 gcggctggcg ttcagcagcc cgctgcgggt tgagcgccgg gaaatttcgc tgaaaacctt
     6361 cccggtgctg gcgaaaacgg acccgctggc ggagtacctg cctccggccg acggcggcgg
     6421 accaaaagcg gatgatgatt ttgcggggct ttactgatga cactggagca gtacaacgtc
     6481 agccacgcga agcggcaggc gcggaacgcg gagaaaacac ggctgaccct gcgctggctg
     6541 cgggaagagc tgtgttccac ggcggagctg gttgcgcgac ggctggggat tgcggcggta
     6601 cagccggttt accgcttcct ggacagtctg gtggcgaaag ggctgctggt ccgggcgaaa
     6661 tacccggttg acggccgtca ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc
     6721 tcgttcgacg aggacgagcc gctgacggac gttattccgt ttcagccgtc aagggtgtcg
     6781 gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc gcctctccat ggagggccgg
     6841 ggggccaccg gctggcggta cctgcaccgt atggccctga aagggatgaa agtgccggac
     6901 gcgctggcgg agctggacgg cagaacggtg gcatttgagg tagagcgcac cgtcaagagc
     6961 cgcaggcggt atcaggaagt ggtgtccggg tatctgttca accgccgggc gaacggcatc
     7021 gatgaaatct ggtacatctg ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt
     7081 ctggcggtgg atgaaatcgt gaatccacag accggcgagg cccgcaaaac ggtggagctg
     7141 gaccgggaga ggctgttcgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg
     7201 ctgtacatgg tatttattga ggacgtggaa gacaaagacg gcgagcgctg cattatgcca
     7261 accagcgagc atctggaaga catggtggtg ctcgcctggc agaccggggc gtatgttttc
     7321 tgttttgtcg accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag
     7381 cggctcaaca gcgacgaaaa agccacgtac tttttcaccg gtgaaaatcc gcaatgggaa
     7441 cgggacaaac ccacaggaat gaagctgtgt gattacctct atcgaaaggt ggagcggttc
     7501 gccatcgaga acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac
     7561 gcgatgattg aggtggtcag cgagcggcac ggggaaagcg cggtgaacga gaagctgaaa
     7621 gcggagctga tgcgggaata taacagccgc tattttaacg tgccggtgcc tgaactgccg
     7681 gaggacaaac ccgcccgccg gtggtggccg ttcggcaaaa aataacgcca gcaccggcat
     7741 ggccggtgct gttattcttc cccgagctta tcccgcatca cctgccagag tgtttccgca
     7801 tcctcgtgaa gccgttcgca catgtcggcc ggggtatcca tgtccagctc gttcagcttt
     7861 tccagcaaca gcagcgactg atccttaatg aatttagcca tattgagggc agttttttcc
     7921 tgcttcgtca tgttctccat tcccttcggt aagttctcag ctaaaccagc cggaccagaa
     7981 gcgttttttc ttctgtgtca gttctgttgt caatctgtcc acttcggcct ggagtttccg
     8041 gcgctcatca tcgtgctggc ggcgttcttc ttcccgggat gttttatctt ccagcatcag
     8101 ggtgacagcc tgccggagat cggccagttc cttttgcatt gccagaagct gttcctgtgg
     8161 gaaatttttt tgtgtatttt cacggtgtga aatgccggga atttgtttct cagggatatt
     8221 cacatttccg tatacacgga tcagttctga cacatccacg acagggttgt ttttcccgtc
     8281 acgtgacacg gtaacctttc cctgttttac atgattgtat agcgttcttc tggtaatgcc
     8341 cgcggcttgt gctgcctgtg aaaggttgag caatgttttc gccactgtat acccctgatt
     8401 ttatcgggtg tgaaaggtgt gtaatttctc acggtaaatg tatcgctgaa ttttacccgt
     8461 ttttccgttc aggatgtggt tttctgacgc gctgcgcttg tcaaaccggc gacggccacg
     8521 caatgaattg cgcccccgtc atcggttatt agaggctgtc tgcccccgga tacccggatt
     8581 ctttctgatg cctgacggtg acgatcacaa tctggtccat ttcccggtcg tggcggtaga
     8641 gcatcacata gccactgtcg ccaaacccga tcaccagttc cttatattcc agcggcagaa
     8701 acggcaccgg gcggccaatg tctggcaatg ttttcagttg ctggatagcc cggacgatca
     8761 cctcacctcc ttttctggct gccagccgat ttttggtttt gagaaagtcc tgaaggcgtt
     8821 gtaaatcgcg ctgtgccagc gcggaaatcg ttacctgtgg catggcggag catcctgctc
     8881 gttctcagtt ccccaggtac ttatccaggc ttccgcttct tccgcagtaa ggtgcagacc
     8941 cgtttcctga tagtgctgcc atgcggcctg tccgtcccgc aggtactggc tgcgcttctc
     9001 ttcccggtcg atgtattcgg tgattgcctc cagcattagc gcgtgcgctg agcggtgccg
     9061 gtcatcggcc agcgttttca gccggtcctt aagttcttca tcgagcctga tggatgttgc
     9121 tgatgacata aaagcccctt tgtagtcata tgtattacac atgactactt taatccgtgg
     9181 cggcgctatt tgccagaaat acggcttttg ctgcccattt ttcagcttcg cttccactaa
     9241 tcagggctgc tacaggctca ctcatcatcc aggactattg cagcctgcgg gttaaaggac
     9301 ctgttactgg cggcaaccat catcattgag cttttgggtg gtgactggtc gcggatcctg
     9361 taagccgtca tttttcagac tgaattcaaa atttataacc tgtcgcattg ccgggtgccc
     9421 tgcgggatat agccagtgcc tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc
     9481 ggtaagcgac acgttgtcca ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg
     9541 accttcgatg cgttttgctg cggcctgtac cgggtaggtt ggctgcagtg tggttacggg
     9601 ttttagtggt atcgcatgta ccggagtgag gaacatggcc gccagcactg tcagagtaaa
     9661 agctttcatg gtctgaaatc cagaggggcg tttttgaata tttgccaggt attttcatcc
     9721 ggtgccggcg gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga
     9781 tctcctcctt cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg
     9841 gtgcacattt ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg
     9901 atggccgcag cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc
     9961 tgggtggtgg aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata
    10021 aagggtatta aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg
    10081 tcactcttta cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca
    10141 aacggcccgc catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg
    10201 tttccggcgg ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc
    10261 ctcagaccgt ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg
    10321 cgctgtggct gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc
    10381 tgcgcccgcc tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt
    10441 tgttttaccg cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg
    10501 acaaaggcgt ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg
    10561 gcctccgggc gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt
    10621 tagctttcag atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata
    10681 aaatttgttg ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg
    10741 gcgctttttt gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag
    10801 gagtaaggta ataattatac tgtgtatatg tacagtgtgc gccgggagac cggttagaga
    10861 tcaaggggtg aaagtccccg accattgaag gaccagcaat ccacaaggtc cccgagtcat
    10921 gcgttgcata ccgcgaggta tggggcgaag cgttgacagg ggtgttgaca ggccagccat
    10981 tgagccacga aatgtatatt aaattaccgg gtgccgacgt tgtactgtta acggaaggca
    11041 acatcatagg gtgcgatact gcgagtgcca catggacccg gcggggtctg agaccctggc
    11101 atgtcaatac gatctctacg cgggaaccgg gagatctccc ctctgaccat ctgccagtgt
    11161 cggagatggc ccgcaccggg aagacgagga gtcatagccg gtgatgtacg gagaggagaa
    11221 gtcggactcg ctcatagtag cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt
    11281 ggagcgaagg agcggggcca aggggaacgc ggaacagcca cacatgcgcc ggacacagag
    11341 ccgggaaagc atgtcacaga ggctgtcacg cgtgcgggaa gctgcgaagc agcggaagaa
    11401 agaacggttt acagcattgt tccacctgct gacagtcgaa gcactggaag ccgcattcct
    11461 ctccctgagc aggaaagcgg ccgccggagt ggatggcatc aggtggatgg actacgccgg
    11521 aaacatgaag aacaacataa cagatctgca ccggaggcta catcagggca gctacagggc
    11581 gcagcccggc aggcgtcact acatcccaaa agcggatgga aaacaacgcc cgctcggcat
    11641 cgcctcgctg gaggacaaga tcgtccagta tgcgctggtg aaaatcctga acgcagtcta
    11701 tgaaaacgac tttatggggt tctcatacgg gttcagaccc gggcgaagcc agcacgatgc
    11761 actggacgca ctggccacag ggctggtacg cactaacgta aactgggtac tggatgccga
    11821 catcagtcag ttcttcgaca gggtgagcca cgaatggctg atcaggttca cagagcatcg
    11881 gatcggcgac cggagggtaa tcaggctcat acgtaagtgg ctcacagccg ggacgtcgga
    11941 ggagggtcaa tggcgagcaa cggaggaagg caccccacag ggtgcggtca tctcaccgct
    12001 gctggcaaac atatacctcc actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg
    12061 ctatgccaca ggcaatgtgg taatggtcag atacgccgat gacatcgtca tcgggttcga
    12121 caaacgatac gatgcccggc gcttccgtat agccatgcag cgcagactga gggagttcgg
    12181 actcacggtt cacccggaga aaacccgtct gatggagttc ggccgcttcg ctgccgaaaa
    12241 ccgtgccatc aggggaaaag gcaaaccaga aacgttcaac ttcctcgggt tcacgcacat
    12301 cagcgggaaa gatcgcaacg gcaggttcat gctgatacga aagacccgcc gggatcggat
    12361 gacggcaact ctgaaagcca tcaaagacgg tctgcgaagg cgctggcatt actcaatccc
    12421 cgaacaggga aaatggctca ggagagtggt tcagggatac ctgaactatc actcggtacc
    12481 gggcaacttc cccaccatgc agaagttcag gacacacgta acaaacctct ggcgccgggc
    12541 gctcaggcgc aggagccaga aggatgatac gacctggacg aaagcaaaca aactggcagc
    12601 cgcatggcta ccaagggttc gggttcttca tccatggcct gtggagcggt tcaccgccag
    12661 acacccgagg caggagcccg gtgcgtaaat cgcgcacgcc gggatctgtg cggggggtat
    12721 ccggtaacgg gtatccctac cgcgac
//
