LOCUS       NZ_CP028563            11840 bp    DNA     circular CON 14-OCT-2023
DEFINITION  Aeromonas hydrophila subsp. hydrophila strain WCHAH045096 plasmid
            p2_045096, complete sequence.
ACCESSION   NZ_CP028563
VERSION     NZ_CP028563.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07821593
            Assembly: GCF_002850695.3
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila subsp. hydrophila
  ORGANISM  Aeromonas hydrophila subsp. hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Aeromonas hydrophila subsp. hydrophila strain
            WCHAH045096
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11840)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11840)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 11840)
  AUTHORS   Wu,W., Liu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/14/2023 03:15:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,853
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 161
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 25 of 111
            Pseudo Genes (incomplete)         :: 84 of 111
            Pseudo Genes (internal stop)      :: 9 of 111
            Pseudo Genes (multiple problems)  :: 7 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028563.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Aeromonas hydrophila subsp. hydrophila"
                     /mol_type="genomic DNA"
                     /strain="WCHAH045096"
                     /isolation_source="Sewage"
                     /sub_species="hydrophila"
                     /db_xref="taxon:196023"
                     /plasmid="p2_045096"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015"
     gene            complement(join(9118..11840,1..247))
                     /locus_tag="C1A23_RS00095"
                     /old_locus_tag="C1A23_00100"
     CDS             complement(join(9118..11840,1..247))
                     /locus_tag="C1A23_RS00095"
                     /old_locus_tag="C1A23_00100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_026383935.1"
                     /translation="MPRRSILSAAERESLLALPDTKDDLIRHYTFSDTDLSIIRQRRGP
                     ANRLGFAVQLCYLRFPGIFLGVDQAPSLPLLKLVADQLKVGVESWDDYGQREQTRREHL
                     VELQTVFGFQPFTMSHYRQAVHTLTDLAMQTDKGIVLASTLVEHLRRQSIILPALNAIE
                     RASAEAITRANRRIYDALAEPLSDTHRRRLDDLLRRRDNGKTTWLAWLRQSPVKPNSRH
                     MLEHIERLKAWQALDLPSGIERSVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVAL
                     AIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEA
                     KQAGRDPFAAIEAVMSWDAFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAV
                     LKLRAAPAAKNVLDAIEVLRGMNTDNARKVPADAPTDFIKPRWQKLVMTDAGIDRRYYE
                     LCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQSSALPLAVATDCDQYLH
                     DRLTLLEAQLATVNRMAAANDLPNAIITESGLKITPLDAAVPDTAQALIDQTAMILPHV
                     KITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTY
                     AKLAWLQAWHTRDETYSTALAELVNAQFRHPFAEHWGDGTTSSSDGQNFRTGSKAESTG
                     HINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGDTKLFIPKGDTAYDALKPMISSDRLNIKAIRA
                     HWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAIALRELGRIERTLFILDWLQSVE
                     LRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVY
                     LERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA
                     "
                     /name="Tn3 family transposase"
     gene            complement(250..810)
                     /locus_tag="C1A23_RS00100"
                     /old_locus_tag="C1A23_00105"
     CDS             complement(250..810)
                     /locus_tag="C1A23_RS00100"
                     /old_locus_tag="C1A23_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636773.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_003055107.1"
                     /translation="MQGQRIGYVRVSSFDQNPERQLEHVEVGRVFTDKASGKDTQRPEL
                     DSLLAFVREGDTVVVHSMDRLARNLDDLRRLVQKLTKRGVRIEFVKESLTFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSGEQIAELKRRVTAGEQ
                     KAKLAREFGVSRETLYQYLKLDQ"
                     /name="recombinase family protein"
     gene            complement(940..1929)
                     /locus_tag="C1A23_RS00105"
                     /old_locus_tag="C1A23_00110"
     CDS             complement(940..1929)
                     /locus_tag="C1A23_RS00105"
                     /old_locus_tag="C1A23_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="WP_010792470.1"
                     /translation="MSASQPIEWTVAQLAQAVERGQLELHYQPIVDLRSEQIVGAEALL
                     RWRHPTLGLLPPGQFLPVIESSGLMPEIGAWVLGAACRQMRDWRVLAWQPFRLAVNVAA
                     SQVGPDFDKWVKGVLADAGLPAAYLEIELTESVAFGDPAIFPALEALRQIGVRFAADDF
                     GTGYSCLQHLKCCPISTLKIDQSFVAGLANDHRDQTIVRTVIQLAHGLGMEVVAEGVET
                     SASLDLLRQADCDTGQGFLFAKPMPAAAFAVFVSQWRGATMNANDPTATSCCVCCKEIP
                     LDAAFTPEGAEYVEHFCGLECYQRFQARAKTGSETDADPNACDSSLSD"
                     /name="DUF3330 domain-containing protein"
     gene            complement(1926..2162)
                     /gene="merE"
                     /locus_tag="C1A23_RS00110"
                     /old_locus_tag="C1A23_00115"
     CDS             complement(1926..2162)
                     /gene="merE"
                     /locus_tag="C1A23_RS00110"
                     /old_locus_tag="C1A23_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="WP_003090697.1"
                     /translation="MNSPERLPTETHKPFTGYLWGALAVLTCPCHLPILAIVLAGTTAG
                     AFIGEHWGIAALTLTGLFVLSVTRLLRAFRGRS"
                     /name="merE"
     gene            complement(2159..2524)
                     /gene="merD"
                     /locus_tag="C1A23_RS00115"
                     /old_locus_tag="C1A23_00120"
     ARG             complement(2163..2524)
                     /name="merD"
                     /gene="merD"
                     /gene_name="mercury resistance co-regulator MerD"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="95.04"
                     /coverage="100.0"
     gene            complement(2542..4227)
                     /gene="merA"
                     /locus_tag="C1A23_RS00120"
                     /old_locus_tag="C1A23_00125"
     CDS             complement(2542..4227)
                     /gene="merA"
                     /locus_tag="C1A23_RS00120"
                     /old_locus_tag="C1A23_00125"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /GO_function="GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="WP_003090700.1"
                     /translation="MTHLKITGMTCDSCAAHVKEALEKVPGVQSAIVSYAKGAAQLALD
                     PGTAPDALTAAVAGLGYKAMLADAPPTDNRTGLFDKVRGWMGAADKGSGGERPLQVAVI
                     GSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESPFD
                     GGMPPTPPTILRERLLAQQQARVEELRHAKYEGILDGNSAITVLHGEARFKDDQSLIVS
                     LNEGGERVVMFDRCLVATGASPAMPPIPGLKESPYWTSTEALVSDTIPERLAVIGSSVV
                     ALELAQAFARLGSQVTILARSTLFFREDPAIGEAVTAAFRAEGIKVLEHTQASQVAHVD
                     GEFVLTTGQGEVRADKLLVATGRTPNTRSLALEAAGVAVNAQGAIVIDKGMRTSSPNIY
                     AAGDCTDQPQFVYVAAAAGTRAAINMTGGDAAINLTAMPAVVFTDPQVATVGYSEAEAH
                     HDGIETDSRTLTLDNVPRALANFDTRGFIKLVIEEGSGRLIGVQVVAPEAGELIQTAVL
                     AIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCAG"
                     /name="merA"
     gene            complement(4299..4574)
                     /gene="merP"
                     /locus_tag="C1A23_RS00125"
                     /old_locus_tag="C1A23_00130"
     ARG             complement(4303..4574)
                     /name="merP"
                     /gene="merP"
                     /gene_name="mercury resistance system periplasmic binding
                     protein MerP"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4590..4940)
                     /locus_tag="C1A23_RS00130"
                     /old_locus_tag="C1A23_00135"
     CDS             complement(4590..4940)
                     /locus_tag="C1A23_RS00130"
                     /old_locus_tag="C1A23_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="WP_001294666.1"
                     /translation="MSEPQNGRGALFTGGLAAILASACCLGPLVLIALGFSGAWIGNLT
                     VLEPYRPIFIGAALVALFFAWRRIYRPAQACKPGDVCAIPQVRATYKLIFWVVAALVLV
                     ALGFPYVMPFFY"
                     /name="mercuric transport protein MerT"
     gene            5012..5446
                     /locus_tag="C1A23_RS00135"
                     /old_locus_tag="C1A23_00140"
     CDS             5012..5446
                     /locus_tag="C1A23_RS00135"
                     /old_locus_tag="C1A23_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance transcriptional regulator
                     MerR"
                     /protein_id="WP_000414383.1"
                     /translation="MEKNLENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIRR
                     YGEADVTRVRFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLQDVREKMTDLA
                     RMETVLSELVFACHARQGNVSCPLIASLQGEKEPRGADAV"
                     /name="mercury resistance transcriptional regulator MerR"
     gene            5758..5997
                     /locus_tag="C1A23_RS00145"
                     /old_locus_tag="C1A23_00150"
     CDS             5758..5997
                     /locus_tag="C1A23_RS00145"
                     /old_locus_tag="C1A23_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepB"
                     /protein_id="WP_101618227.1"
                     /translation="MQEKEAKTIFDMGGFSKAMIVPTPMGAGFHLHLVRFGKGGTEVVE
                     RQRGGWRVFSSMDAAANTANGIGFRRIEIDLSSR"
                     /name="plasmid replication protein RepB"
     gene            complement(6182..7168)
                     /locus_tag="C1A23_RS00150"
                     /old_locus_tag="C1A23_00160"
     CDS             complement(6182..7168)
                     /locus_tag="C1A23_RS00150"
                     /old_locus_tag="C1A23_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_101618223.1"
                     /translation="MALSFSLAIDKMQAPAIGRAEGHNLRLHKTESQLRPEAWFTPKGR
                     HQVEPWRPEVLAKAKGLAKRKDAVQAIQFVIQLGNQTDWRGEPEPDFPEGRPIDITDPI
                     NLAGKGIRAWVEKEFGDENCVGIDLHTDESSPHFHVVVTPIKDGKLQAKAWLDGASKVA
                     ALRKRAWQSVNAYIACEYKPGAAGGAPHDPRKAAGQVPAPSILDKATGHAKAKRLEREN
                     VALQEENARLKQALFSRQKGRYSADLLEKTKQAELTAENAQRALGEALQEILALRLGAG
                     QHEQTVKRLETEIQKLEGYRNDLSDQLNEAEERLRELEPEQHRGPRP"
                     /name="plasmid recombination protein"
     gene            7618..8256
                     /locus_tag="C1A23_RS00155"
                     /old_locus_tag="C1A23_00165"
     CDS             7618..8256
                     /locus_tag="C1A23_RS00155"
                     /old_locus_tag="C1A23_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014045.3"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_101618221.1"
                     /translation="MRTMVFVDYWNLQLSLQHEDAAAQGLNGAALQQHRFSIDWFSLGL
                     ELTNLAIANASPDPANPLPLTFQETRIYTSTNPADQGYKQWATRTLGSRPGVRVFCLDR
                     KPKRNPNCPACHREMDVCPHCRAVINATQEKGVDTLLVTDLLRLGLDNSYDAAILVSQD
                     ADMAPAAAHLASKGIKVIHAGIRHFGVGVGKECWASFDLFAHRARIQRV"
                     /name="NYN domain-containing protein"
     ARG             5013..5443
                     /name="merR"
                     /gene="merR"
                     /gene_name="mercury resistance transcriptional regulator
                     MerR"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4594..4940)
                     /name="merT"
                     /gene="merT"
                     /gene_name="mercuric transport protein MerT"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5756..5997
                     /name="rep_cluster_2020"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.243"
                     /coverage="100.0"
                     /evalue="2.96e-72"
ORIGIN
        1 gctggtcggc gaccagtttc agtaggggca aagacggcgc ctgatcgaca ccaaggaaga
       61 ttccgggaaa gcgcaggtag cagagctgca cggcaaagcc caagcgattc gcaggcccgc
      121 gccgctggcg gatgatggag agatcggtat cgctgaacgt gtaatgtcga attaggtcgt
      181 ccttggtgtc cggcaacgcc aacaggcttt cccgctcggc ggcggacagt atggaacgac
      241 gaggcatatt tattgatcca atttcaagta ttgatacagg gtctcccggc tgacaccaaa
      301 ttcacgggcc agcttcgctt tttgctcgcc cgccgtgacg cgccgtttca gttcagcaat
      361 ctgttcaccg ctcagagact tctttcggcc tcggtaggct ccgcgttgct tagcgagcgc
      421 gatgccttcg cgctgccgct cacggatcaa ggcccgctcg aattcggcga atgcccccat
      481 gaccgacagc atcaggttcg ccatcggtga atcctcgccg gtgaaggtca ggctttcctt
      541 gacgaactcg atgcgcacgc cgcgcttggt cagcttttgt acgaggcggc gcaagtcatc
      601 gaggttgcgc gccaagcgat ccatgctgtg aaccaccacg gtgtcgcctt cgcgcacgaa
      661 ggccagcagc gaatcaagct cgggccgctg ggtgtccttg cccgacgcct tgtcggtgaa
      721 caccctgccg acttcgacat gctccagttg ccgctccggg ttctggtcga agctgctgac
      781 ccggacgtag ccgatgcgtt gaccctgcaa gatacctcca aatacgaaag tgtcaggaag
      841 aaatctatga cctttcgccg caggtgtcaa gaaacacaaa agttaactct attctgacgt
      901 tgctgggtgg cttctcctga catcaggata gggtatgcct cagtctgaca gtgatgagtc
      961 gcaggcgttc ggatcggcat cggtttcgct cccggtcttg gcgcgcgcct ggaagcgctg
     1021 atagcactcc agcccgcaga agtgctcgac gtattccgcg ccttccgggg tgaaggcggc
     1081 atcgagcggg atttccttgc agcacacgca gcaactggtg gcagtcggat catttgcatt
     1141 catggtggca cccctccatt gactgacgaa gacggcgaat gccgccgccg gcatcggctt
     1201 ggcgaacagg aagccttgtc ctgtgtcgca gtccgcttgt cgcaacaaat caagactcgc
     1261 cgatgtttcc acgccttcgg ccactacttc catgccaagc ccatgcgcaa gctgaatcac
     1321 ggtgcgcacg atggtctggt cgcggtggtc gttggcgagt ccggcgacaa acgattggtc
     1381 gatcttgagc gtgctgatgg ggcagcattt caggtgttgc aggcaggaat agccggtgcc
     1441 gaagtcgtcg gcggcgaagc gcacaccgat ctgtcgcaaa gcttccaggg cggggaagat
     1501 cgccggatca ccgaacgcaa ccgattcggt cagctcaatt tcaagatacg cggcgggcaa
     1561 cccggcatcg gccagcacgc cctttaccca cttgtcgaaa tctggcccca cttggctcgc
     1621 cgcaacattg acggccagcc ggaacggttg ccatgccagc acccgccagt cgcgcatttg
     1681 acggcaggct gcgcccagca cccatgcgcc gatttccggc atcaggccgg acgattcgat
     1741 cacgggcagg aactggcccg gcggcaacag tccgagcgtc ggatgacgcc agcgcaacag
     1801 ggcttccgcg ccgacaatct gctcactgcg caaatcgaca atcggctggt agtgcagctc
     1861 aagctgcccg cgctcgaccg cctgcgccag ttgcgccact gtccattcaa ttggctggga
     1921 agcgctcatg atcgacctct gaaggcccgc agcagccgcg tcacagacag gacaaacaag
     1981 ccggtcagcg tgagggctgc aataccccag tgctcgccga tgaacgcgcc ggccgtcgtg
     2041 ccggccagca caatggcgag aatcggcaaa tgacagggac aggtgagcac cgccagcgca
     2101 ccccacaggt agccggtgaa cggcttgtgt gtctcggtcg gcaagcgctc ggggctgttc
     2161 atggcagact ctccgcttgc tgggccggcg cggtcggcat cgcggccaac tgcacttcca
     2221 gatcggccaa cgcttcgcgt cggcgttcga cgaactgact cagcacggca agctgtgcgg
     2281 ctgtttcatc gcagtccgcc gcatccagcg cccggcacaa ccgcgccaat gcgtccaggc
     2341 cgatgcccgc ctcaaaagcg gcccgcacaa agcacagccg ctgcaaggcg gcgtcatcga
     2401 acaagccgta gccgcccgtg gtgcaggcga ctggccgcag caatccgcgc agcaggtagt
     2461 cgcgcacgat atgcacgctc accccggcct caagggccag tcgggacact gtgtaggcgt
     2521 tcatcgaaca cctccttttc cttatccggc gcagcaggac agttgcttca cgtccttgct
     2581 gaaggtctgc gccgcgagct tcaagccctc gaccatcgtt aggtagggga acaactggtc
     2641 ggctagttcc tgcaccgtca tgcggttgcg aatggcgaga acagccgtct ggatcagttc
     2701 gcccgcttcc ggggccacca cctgtacgcc aatcagccgt ccgctgcctt cctcgatgac
     2761 cagcttgatg aagccgcggg tatcgaagtt ggcgagcgca cgcggcacgt tgtcgagcgt
     2821 cagcgtgcgg ctgtcggttt cgatgccatc gtggtgcgct tccgcctcgc tgtagcccac
     2881 ggtcgccact tgcgggtcgg tgaacaccac ggccggcatc gcggtcagat tgatggcggc
     2941 gtcgccgccg gtcatgttaa tggccgcacg agtgccggcc gctgccgcca catagacgaa
     3001 ctgcggctgg tcggtgcagt cgccggccgc gtagatgttc gggctactgg tgcgcatgcc
     3061 cttgtcgatg acgatggccc cctgcgcatt gacggctacc cccgccgctt ccaatgccag
     3121 gctgcgcgtg ttcggtgtcc ggccggtggc gaccagcagc ttgtcggcgc gcacttcgcc
     3181 ctgtccagtg gtcagcacga attcgccgtc cacatgggcg acttggctgg cttgcgtatg
     3241 ttccagtacc ttgattcctt cggcacggaa ggcggctgtg acggcctcgc cgatggcagg
     3301 gtcttcgcgg aagaacagcg tgctgcgcgc aaggatcgtg acctggctac ccagccgggc
     3361 gaaggcttgc gccagttcca gcgccaccac cgacgagccg atgacggcca ggcgttcggg
     3421 aatggtgtcg ctgaccaagg cctcggtcga agtccagtag ggtgactctt tcaggcccgg
     3481 aatcggcggc atggccggac tggcacccgt ggcgaccagg cagcggtcga acatcacgac
     3541 gcgctcacca ccctcgttca aactaacgat aaggctctgg tcgtccttga aacgcgcttc
     3601 accgtgcaga acggtgatgg ctgaattgcc gtccaggatg ccttcgtact tggcatgacg
     3661 gagttcttcg acacgggcct gctgctgggc cagcagccgc tcgcgcaaga tcgtcggcgg
     3721 tgtgggtggc atgccgccgt cgaatgggct ttcccggcgc agatgggcga tgtgggcggc
     3781 gcggatcatg atcttggacg gcacacaacc gacgttgacg caggtgccgc cgatggtgcc
     3841 gcgctcaatc agcgtgacct gcgcgccttg ctcgacggcc ttcagtgctg ccgccatcgc
     3901 ggctccaccg ctgccgatca cggcgacttg caacgggcgc tcgccgccgc tgcccttgtc
     3961 ggcggcaccc atccagccgc gcaccttgtc gaacagccca gtgcggttgt cggtcggcgg
     4021 ggcatcggcg agcatcgctt tgtagcccag gccagccacg gcggcggtca gtgcgtccgg
     4081 cgctgtgcct ggatcaaggg cgagctgggc cgcgcccttg gcataggaca ctatggcaga
     4141 ttggacgccg ggtacttttt ccagcgcttc cttgacgtgc gccgcgcacg agtcgcaggt
     4201 catgccggtg atttttagat gggtcatgcg acagatcctt tttcgtttgt ggtagcagac
     4261 aagggcgtca gatgtgctgc gctgccgttt tcagtgcctc acttcttgac gctggacgga
     4321 tagcctgcgt cttcggtggc cttggtcagc ttctgcacgc tggtcttggc atcatcgaag
     4381 gtgacaaccg cttcgcgtgt ctcgaaggtc acgttaactt tgctgacgcc ttcgaccttg
     4441 gaaatcgcct tcttgacggt gatcggacaa gcggagcagg tcatgcccgg tacggacagc
     4501 gtgacggtct gggtggcggc ccacacgggg gcaacaacgg cagcgatggc gagagaggca
     4561 aacagttttt tcatgatgaa ctcctgtgat taatagaaaa atggcatgac gtagggaaat
     4621 ccgagcgcga ccagaaccag cgcggccacg acccagaaaa tgagcttgta agtagcgcgc
     4681 acttggggaa tcgcacacac atcccctggt ttgcaggctt gcgccggtcg gtagatgcgc
     4741 cgccaggcga aaaacagcgc caccaacgcc gcgccgatga agatcgggcg ataaggttcc
     4801 aacaccgtca agttgccgat ccaagcgccg ctgaacccca gggcgatcag aaccagcggc
     4861 cccaggcagc aagccgaggc gaggatggcg gctagcccgc cagtgaagag cgccccgcgc
     4921 ccgttttgag gttcagacat acgcttgtcc tttcaaatct ggattggata gcttaagctt
     4981 acttccgtac caatgtacgg agtcaagcga tatggaaaaa aatttggaga atctgactat
     5041 tggcgttttc gccaaggcgg ccggggtcaa cgtggaaaca atccggttct atcagcgcaa
     5101 gggcttgttg ccggagccgg acaagcccta tggcagcatc cgccgctatg gcgaggcgga
     5161 tgtgacgcgg gtgcgcttcg tgaaatcagc ccagcggctc ggattcagcc tcgacgagat
     5221 cgcagagctg ctgaggctgg atgacggcac ccactgcgag gaagccagca gcctggccga
     5281 gcacaagctt caggacgtgc gcgaaaaaat gaccgacctg gcgcgcatgg aaaccgtgct
     5341 atccgaactt gtgttcgcct gccatgcgcg gcaagggaac gtttcttgcc cgctgattgc
     5401 ttcgctgcaa ggggagaaag agccgcgtgg tgccgacgcg gtgtagccga gggtagttac
     5461 gccttagcgt gctttatttt ccgttttctg agacgacccc catgcggcca gcgcctgtgc
     5521 gatctgacgg agatgcagtt gctcgatttt ggggggcgga tcagcgttga aggggggctg
     5581 cttaacttcg atttggacgc cgcagagtgg cgcgaacgcc tgattacctt gggccttgag
     5641 cctcattgaa aatcattttg tggatttgaa taatcatatt gccaaagtga ttgactgcac
     5701 cccccaagag ggtaacatcg aattgtagat ttgatttttc aaatggggga tttgatgatg
     5761 caggaaaaag aggccaagac cattttcgat atgggcggat tctccaaggc gatgatcgtc
     5821 cccaccccaa tgggggcggg gttccacctc catctggtcc gcttcggcaa gggcggcacc
     5881 gaggtcgtgg agcgccagcg cggaggctgg cgggtcttct cctcgatgga tgctgccgcc
     5941 aacactgcca acggcattgg ctttcgccgc atcgagatag acctcagcag ccgctagggc
     6001 ctagcccagc gcagcagggc ggcaaacgcc gccatccatg cggccccttc gaagtatgag
     6061 ggggcctccc acccgaagaa gtcatgcagc cccagcaggc ccataaacca gccgccagag
     6121 gcagttacag cacctttcat gaagaacact gcggcgaaca gcgccgctac gtgccaagcc
     6181 ttcatgggcg cggccctcgg tgctgctctg gctccagctc gcgcagccgt tcttcggcct
     6241 cgttaagctg atcggacaga tcattgcggt agccctccag cttctggatc tcggtttcca
     6301 accgcttcac tgtctgctcg tgctgcccag cacccaacct cagcgccaaa atctcctgca
     6361 gggcctcgcc taacgctctc tgagcgtttt ccgcagttag ctctgcctgt tttgtcttct
     6421 caagcaggtc tgcgctatag cggcctttct ggcggctaaa caacgcctgt ttgagtcggg
     6481 cattctcctc ctgcagggct acattttcgc gctccagccg cttggccttg gcgtgtccgg
     6541 ttgccttatc gagaattgat ggcgccggca cctgccccgc cgccttgcgg ggatcgtgag
     6601 gtgcgccgcc agcggcccca ggtttgtact cgcaggcgat gtacgcattg acggactgcc
     6661 atgcccgttt ccgcaaggcg gccaccttgc ttgccccgtc taaccaagcc tttgcctgta
     6721 gcttgccgtc cttgatcggc gtcacgacga catgaaaatg agggctgctc tcgtcggtgt
     6781 ggagatcgat ccctacgcag ttctcgtccc caaactcctt ctccacccat gccctgatcc
     6841 ctttgcctgc gaggttaatt ggatcggtaa tgtcgatggg cctcccctca gggaaatccg
     6901 gttcaggttc acctcgccag tcggtttggt tgccgagctg gatcacgaac tggatcgcct
     6961 gcacagcatc cttgcgcttt gccaacccct tggcctttgc cagcacctcc ggtcgccatg
     7021 gttcgacctg atggcggcct tttggggtaa accatgcttc cggtcggagc tgactttccg
     7081 ttttgtggag gcgcaggtta tgcccctctg ctctgccgat cgcaggggcc tgcatcttgt
     7141 cgattgctag cgagaatgag agtgccatag tgtctttgcc tcctgctatt ttgtctctgg
     7201 ttatagtacc agagttggga agcaaagtgt tctcctaaca ctttgtgcca aagttggagc
     7261 ccctgcgggg ggctaaaacc aaacatgttt tgcgctgtta tagggggggg cgattatgcg
     7321 taagcagggg gcgaaaaaac tgccgcgcta cgcttgccgc ctcccaaaca gaagtaggca
     7381 gactgcgcta gcctccccct gtttgggagg ttttagcgaa cagaaacacc gtgcatttca
     7441 ttgctgaaat ctcaacgaga agagttgggg tttgttctcg gagggggggc aaaatatggt
     7501 tcgtcatcat ttagatgagc ataaaaataa aggggccgaa cggcccctag ggtatcatag
     7561 aaccggagac ttattctccg gtaggcaata atggtgcaat tataggtatc gaatcttatg
     7621 agaacaatgg tttttgttga ttattggaat ctgcagttat ctcttcagca cgaagatgcc
     7681 gcggcacaag gtctcaacgg cgcagcgttg cagcaacatc gatttagtat cgattggttt
     7741 tctttgggtc ttgaactaac gaatttagct attgcaaatg catctcccga tccagctaat
     7801 ccactaccgt taacctttca ggaaacaaga atttacacat caactaaccc tgctgatcag
     7861 gggtataagc agtgggcaac gagaactctt ggcagtcgcc ctggagtccg agttttttgt
     7921 ttggatcgca agccaaaaag aaacccaaat tgtcctgcct gccaccgaga aatggatgtc
     7981 tgtccacact gcagagcagt gattaatgct acgcaagaaa agggagtgga taccctgctt
     8041 gttacggacc ttttgcgtct cggattggat aattcttatg atgcagccat tcttgtttca
     8101 caagatgcag acatggcccc tgccgctgcg cacttggcat ctaaaggcat caaagtaatt
     8161 catgctggga tccggcactt tggggttggt gttggtaaag agtgttgggc tagctttgat
     8221 ctgttcgctc atagagctcg gatccagcgg gtataacttg atcatatcgt ctgatccagc
     8281 gtaacctgtt tcgcagaaac gaaaaaaccg ccctgcaagg cggtttgttc gaaggtggaa
     8341 tcagttgggg aactgacttc acctggtaac ggctccgtgc gggaagcagc taccgtaaga
     8401 ccctttcagt ttagcggcca ctcggccatt caatcaagct tttcccgcag caagacccgt
     8461 taccgaaggc cgccaccttt ggcgttttgt cgtacccaca cggattggaa gcatgagttt
     8521 gttgccgtgt gtgggtgtgg tcggtcgggc agaacgggga aacctcacat actgcccagc
     8581 gtgcgacgcg caccatagcc tcccaaggct cgcaaccttg gggtaccagc accgagcgta
     8641 acggggaacc aaccacggcc agctcgataa aaacggcgcg gcagtccgac ccacaggtgg
     8701 ggactgaagc aggtacagag tggcaaggtg tgtctcagga gcgcgtgagg gggagctgcc
     8761 gcaagcagcc tcgatagtcc tgagcaaggc gagctatcca gcgtaactac gtcattttta
     8821 acgtagccaa gcaggagggc gagcctatct tgaagcgcag cgaaagatag gagagccggt
     8881 gtcttacgcc ctgtctttca aacaaaaaaa gagagatgac cttggcgagg tgggtaactg
     8941 cgcccgtccg tccacatgcc atcggttcgg cttgtggacg gcagcagtta tccacggaag
     9001 cgggtttggc tggaggtacg tatgagcttc gacaagcacg ggattgaggt cgatggggat
     9061 tgcatctggc tactggacgc atgcggggtc gcctcagaaa acggaaaata aagcacgcta
     9121 agccggttgc aacggtcgta gcggcctgaa cttgcccgcg ccgatcttgg cgctgctgcg
     9181 ccagaggtaa tcgccggtca ggttgatgtg ttcccagccg agcggcgaca ggtattgcaa
     9241 tagcgcgtca tcgacagcat gcccgttgcc acgcaacgca tgtgctgcgc gctccagata
     9301 gaccgtgttc cacaacacga cggccgccgt caccaggttg aggccgctgg cccggtagcg
     9361 ctgctgctcg aagctgcggt cgcggatttc gcccagacgg ttgaagaata cggcgcgggc
     9421 cagcgcgttg cgcgcttcgc ccttgttcag tccagcgtgg acgcggcggc gcagctccac
     9481 gctttgcaac cagtccagga tgaacagcgt gcgttcgatg cgccccagct cgcgcagggc
     9541 gatggctaag ccgttctggc gcggatagct gccgagtttc cgaagcatca gcgaggctgt
     9601 caccgtgccc tgcttgatcg aggtcgccag ccgcagaatt tcatcccagt gggcacgaat
     9661 agccttgatg ttcagcctgt cgctgctaat catcggtttg agcgcgtcat aggcggtatc
     9721 ccccttgggg atgaacagct tggtgtcgcc caggtcacgg atgcgcggcg caaagcggaa
     9781 gcccaggaga tgcatcaggg caaagacgtg atcggtgaag cctgccgtgt cggtgtagtg
     9841 ttcctcgatg cgcaggtcgg actcgtggta cagcaggccg tcaagtacat aggtcgagtc
     9901 gcgcacgccg acattgacca ccttggtgtg gaacggcgcg tactggtcgg atatatgggt
     9961 gtagaaggtt cgcccagggc tgctgccata tttcgggttg atgtgaccgg tactctcggc
    10021 cttgctcccg gttcggaagt tctggccgtc cgacgatgac gtggtgccgt cgccccagtg
    10081 ttcggcgaag ggatgccgga attgggcgtt caccaactcg gccagcgctg tcgaataggt
    10141 ttcgtcgcgg gtatgccagg cttgtagcca ggcgagcttg gcgtaggtcg tgccggggca
    10201 ggactcggcc atcttggtca ggcccaggtt gatcgcgtcg gccaggatgg tggtcaacag
    10261 cagatttttg tccttggcca gatcgcccga tttcaggtgc gtgaagtggc gggtgaagcc
    10321 cgtccactca tcgacttcga gcagcagttc ggtgatcttg acgtgtggca ggatcatggc
    10381 tgtctggtcg atcagcgcct gcgcggtgtc gggtaccgcc gcatccagcg gcgtgatctt
    10441 caggcccgac tcggtgatga tcgcattcgg caggtcgttg gccgctgcca tgcggttgac
    10501 cgtggcaagc tgcgcttcaa gcagcgtcag gcggtcgtgc aggtactggt cgcagtcggt
    10561 ggccacggcc agcggcaatg cgctggactg cttgaggctg gcgaacttcg cgggcggcac
    10621 caggtagtcc tcgaagtcct tgaactggcg cgatccctgc acccagatgt cgcccgagcg
    10681 cagcgagttc ttcagctcgg acagcgcgca cagctcgtag tagcgccggt cgatacccgc
    10741 gtcagtcatc accagcttct gccagcgcgg cttgatgaag tcggtcgggg catcggcagg
    10801 cactttgcgg gcgttgtcgg tgttcatgcc acgcagtacc tcgatggcat cgagcacgtt
    10861 cttggcggcg ggtgcggccc gcagcttgag cacggcaagg aattccggcg cgtagcggcg
    10921 caaggtggcg taactttcgc cgatgcggtg caggaaatcg aagtcatcgg gctgagcgag
    10981 cttctgcgcc tcggtgacgc tttcggcgaa ggcatcccag gacatgacgg cctcgatggc
    11041 ggcgaacgga tcgcggccag cttgcttggc ctcgatcagc gcctggccga tgcgcccgaa
    11101 cagccgcacc ttggcgttga tcgccttgcc ggatgcctga aactgctgct gatgcttgtt
    11161 tttggcggcg ttgaacagct tgcccaggat gcggtcgtgc aagtcgatga tttcgtcggt
    11221 gaccgtggcc atgccctcga tggccagcgc cacgagggtg gcgtagcgcc gttgcggctc
    11281 gaacttggct aagtcggcgg gcgtcatctg gccaccctca cgggcgatct tgagcaggcg
    11341 gttctggtgc accgaccgct cgataccgga aggcagatcg agcgcctgcc acgccttgag
    11401 gcgttcgatg tgttccagca tgtgccgcga gttcggttta accggcgact ggcgcagcca
    11461 ggccagccag gtcgtcttgc cgttgtcccg gcgcctgagc agatcgtcga ggcggcggcg
    11521 atgcgtgtcc gacagtggtt cggccaaggc gtcatagatg cgccggttgg cgcgagtgat
    11581 ggcctcggcg ctagcgcgct cgatggcgtt gagcgcgggc aggatgatcg actgtcgccg
    11641 cagatgctca accaaggtgc tagccagcac gatgcctttg tcggtttgca tggccaggtc
    11701 ggtcagcgtg tgcacggcct gccggtagtg gctcatggtg aacggctgga agccaaacac
    11761 cgtttgcagc tcgaccaggt gctcgcgccg ggtctgctcc cgctgcccgt agtcgtccca
    11821 gctttcgacg ccgaccttga
//
